金花茶组培苗产业化生产技术研究
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摘要
金花茶无菌芽接种于优化后的培养基中增殖、生根、移栽、培育.结果表明:丛芽在增殖培养基中添加L-半胱氨酸10.0 mg/L、叶酸0.1 mg/L、生物素60.0 mg/L时,增殖系数高达11.58;在含ABT13.0 mg/L、ABT61.0 mg/L、KH2PO4100.0 mg/L的生根培养基中,在800～6 000 lx光照强度下生根率为96.67%;在乔木郁闭度0.4～0.6的条件下,黄心土:发酵后鸡粪=7:1中苗木移栽成活率可高达99.60%,一年生苗高达56 cm,两年生苗高达105 cm,苗相整齐,已达到产业化要求.
The clustered buds of Camellia nitidissima were inoculated in the optimized medium for multiplication, rooting transplanting and cultivation. The results show that: single bud in the proliferation medium containing L-cysteine 10.0 mg/L,folic acid 0.1 mg/L and biotin 60 mg/L, multiplication coefficient of reached 11.58; in rooting medium containing ABT13.0 mg/L,ABT61.0 mg/L and KH2 PO4100.0 mg/L, the rooting rate was 96.67% under 800-6 000 lx light intensity; Under the canopy closure of the trees 0.4～0.6, yellow soil: fermented chicken manure=7:1, the survival rate of transplanting seedlings can reach99.60%. Annual seedlings are 56 cm high, and the two-year-old seedlings up to 105 cm, neat, has reached the requirements of industrialization.
The clustered buds of Camellia nitidissima were inoculated in the optimized medium for multiplication, rooting transplanting and cultivation. The results show that: single bud in the proliferation medium containing L-cysteine 10.0 mg/L,folic acid 0.1 mg/L and biotin 60 mg/L, multiplication coefficient of reached 11.58; in rooting medium containing ABT13.0 mg/L,ABT61.0 mg/L and KH2 PO4100.0 mg/L, the rooting rate was 96.67% under 800-6 000 lx light intensity; Under the canopy closure of the trees 0.4～0.6, yellow soil: fermented chicken manure=7:1, the survival rate of transplanting seedlings can reach99.60%. Annual seedlings are 56 cm high, and the two-year-old seedlings up to 105 cm, neat, has reached the requirements of industrialization.
引文
[1]傅立国.中国植物红皮书———稀有濒危植物（第一册）[M].北京：科学出版社，1992.
[2]梁盛业．金花茶[M]．北京：中国林业出版社，1993.
[3]廖汉刃，周传明，董学军，等．金花茶组织培养及其试管苗嫁接繁殖试验初报[J]．广西农学学报，1987(2):66-70.
[4]颜慕勤，陈平，王以红，等．广西七种金花茶的组织培养快速繁殖[J]．实验生物学报，1988,21(1):1-4.
[5]黄晓娜，叶品明，黄连冬，等．金花茶组培苗试管外生根研究[J]．安徽农业科学，2014,42(18):5751-5752.
[6]李桂娥，李志辉，罗燕英，等．金花茶组培快繁体系的建立[J]．农业科学研究，2017,38(3):17-20,24.
[7]吴丽君，高楠，陈达，等.金花茶杂交种子无菌苗的组培快繁研究[J].南京林业大学学报（自然科学版），2018,42(5):32-38.
[8]刘芳，俞建妹，刘玉军，等.金花茶组培芽不定根诱导技术研究[J].安徽农业科学，2018,46(25):84-86.
[9]龙敏，沈遐，刘芳，等.影响金花茶组织培养芽增殖生长的几种因素[J].绿色科技，2018(21):96-98.
[10]杨成华，张廷忠，方小平.贵州2种金花茶的扦插繁殖试验[J].林业科技通讯，1996(10):23-24.
[11]龚弘娟，胡兴华，李洁维，等.3种不同基质对金花茶扦插的影响[J].福建林业科技，2009,36(3):145-147,157
[2]梁盛业．金花茶[M]．北京：中国林业出版社，1993.
[3]廖汉刃，周传明，董学军，等．金花茶组织培养及其试管苗嫁接繁殖试验初报[J]．广西农学学报，1987(2):66-70.
[4]颜慕勤，陈平，王以红，等．广西七种金花茶的组织培养快速繁殖[J]．实验生物学报，1988,21(1):1-4.
[5]黄晓娜，叶品明，黄连冬，等．金花茶组培苗试管外生根研究[J]．安徽农业科学，2014,42(18):5751-5752.
[6]李桂娥，李志辉，罗燕英，等．金花茶组培快繁体系的建立[J]．农业科学研究，2017,38(3):17-20,24.
[7]吴丽君，高楠，陈达，等.金花茶杂交种子无菌苗的组培快繁研究[J].南京林业大学学报（自然科学版），2018,42(5):32-38.
[8]刘芳，俞建妹，刘玉军，等.金花茶组培芽不定根诱导技术研究[J].安徽农业科学，2018,46(25):84-86.
[9]龙敏，沈遐，刘芳，等.影响金花茶组织培养芽增殖生长的几种因素[J].绿色科技，2018(21):96-98.
[10]杨成华，张廷忠，方小平.贵州2种金花茶的扦插繁殖试验[J].林业科技通讯，1996(10):23-24.
[11]龚弘娟，胡兴华，李洁维，等.3种不同基质对金花茶扦插的影响[J].福建林业科技，2009,36(3):145-147,157