产甘油假丝酵母HOG途径应答研究进展
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摘要
产甘油假丝酵母(Candida glycerinogenes)是工业甘油生产菌株,具有多重高抗逆、生长迅速、糖代谢高效等优点,是优良的工业宿主菌株.高渗甘油(high osmolarity glycerol, HOG)应答途径是真核细胞应答高渗透压胁迫的关键响应机制.本文从产甘油酵母HOG途径的生物信息学分析、MAP激酶Hog1对细胞表型、甘油转运和合成、氨基酸的合成与转运调控进行阐述,为进一步理解该酵母的HOG应答途径和抗逆机制奠定了基础.
Candida glycerinogenes has been applied in the bioproduction of glycerol at industrial scale. It is an excellent industrial host with high tolerance to multistress, rapid cell growth, and glucose consumption. High osmolarity glycerol pathway(HOG) is a key regulatory mechanism in the stress response of eukaryotic cell. In this review, we summarize the components of HOG pathway and the effects of protein HOG1 on cell phenotype and the transportation and synthesis of glycerol and amino acids in C. glycerinogenes to further understand its HOG pathway and stress tolerance mechanism.
Candida glycerinogenes has been applied in the bioproduction of glycerol at industrial scale. It is an excellent industrial host with high tolerance to multistress, rapid cell growth, and glucose consumption. High osmolarity glycerol pathway(HOG) is a key regulatory mechanism in the stress response of eukaryotic cell. In this review, we summarize the components of HOG pathway and the effects of protein HOG1 on cell phenotype and the transportation and synthesis of glycerol and amino acids in C. glycerinogenes to further understand its HOG pathway and stress tolerance mechanism.
引文
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3 Zhuge B,Guo X N,Mawadza C,et al.A novel Candida glycerinogenes mutant with high glycerol productivity in high phosphate concentration medium.World J Microbiol Biotechnol,2005,21:453-456
4 Song B P.The Physiological and Biochemical Characterisics and Ploidy of C.glycerinogenes and Its Metabolism of Different Carbon Sources(in Chinese).Dissertation for Master’s Degree.Wuxi:Southern Yangtes University,2012.1-47[宋保平.产甘油假丝酵母生理生化特性,倍性及不同碳源发酵代谢的研究.硕士学位论文.无锡:江南大学,2012.1-47]
5 Ji H,Lu X,Wang C,et al.Identification of a novel HOG1 homologue from an industrial glycerol producer Candida glycerinogenes.Curr Microbiol,2014,69:909-914
6 Yang F,Lu X,Zong H,et al.Gene expression profiles of Candida glycerinogenes under combined heat and high-glucose stresses.J Biosci Bioeng,2018,126:464-469
7 Xie T.Effects of Phosphate-limitation on glycerol biosynthesis and intracellular phosphorus accumulation of Candida glycerinogenes(in Chinese).Microbiol China,2009,36:1289-1293[谢涛.限磷对产甘油假丝酵母甘油合成与胞内磷积累的影响.微生物学通报,2009,36:1289-1293]
8 Xie T,Fang H Y,Zhuge J.Effects of phosphorus sources on the production of Candida glycerinogenes fermentation and kinetic analysis(in Chinese).J Chem Ind Eng,2005,56:2404-2409[谢涛,方慧英,诸葛健.磷源对产甘油假丝酵母发酵生产甘油的影响及动力学分析.化工学报,2005,56:2404-2409]
9 Xie T.Stimulatory effects of some amino acids on glycerol production by Candida glycerinogenes(in Chinese).Chin J Biotech,2006,22:138-143[谢涛.一些氨基酸对产甘油假丝酵母甘油生产的促进作用.生物工程学报,2006,22:138-143]
10 Jiao C,Zhuge B,Fang H Y,et al.Effects of dissolved oxygen on metabolic flow distribution in glycerol-producing fermentation(in Chinese).JChem Ind Eng,2012,63:1156-1167[焦策,诸葛斌,方慧英,等.溶氧对产甘油发酵代谢流分布的影响.化工学报,2012,63:1156-1167]
11 Xie T,Fang H Y,Zhuge J.Mechanism of corn steep liquor during glycerol fermentation by Candida glycerinogenes(in Chinese).Microbiol China,2006,33:80-84[谢涛,方慧英,诸葛健.玉米浆在产甘油假丝酵母甘油发酵中的作用机理.微生物学通报,2006,33:80-84]
12 Xie T,Fang H Y,Zhuge J,et al.Effects of osmotic pressure on glycerol synthesis and intracellular phosphorus accumulation in Candida glyceropoiesis(in Chinese).J Chin Biotechnol,2009:61-66[谢涛,方慧英,诸葛斌,等.渗透压对产甘油假丝酵母甘油合成与胞内磷积累的影响.中国生物工程杂志,2009:61-66]
13 Xie T,Fang H Y,Zhuge J,et al.Effects of temperature on the fermentation process of Candida glyceropoiesis(in Chinese).Chem Eng,2009,37:55-58[谢涛,方慧英,诸葛斌,等.温度对产甘油假丝酵母产甘油发酵过程的影响.化学工程,2009,37:55-58]
14 Krantz M,Becit E,Hohmann S.Comparative genomics of the HOG-signalling system in fungi.Curr Genet,2006,49:137-151
15 de Nadal E,Ammerer G,Posas F.Controlling gene expression in response to stress.Nat Rev Genet,2011,12:833-845
16 Krantz M,Becit E,Hohmann S.Comparative analysis of HOG pathway proteins to generate hypotheses for functional analysis.Curr Genet,2006,49:152-165
17 de Dios C H,Roman E,Alonso Monge R,et al.The role of MAPK signal transduction pathways in the response to oxidative stress in the fungal pathogen Candida albicans:Implications in virulence.Curr Protein Peptide Sci,2010,11:693-703
18 Ji H.Candida glycerinogenes HOG Reply Way and Osmotic Tolerance Mechanism Research(in Chinese).Dissertation for Doctoral Degree.Wuxi:Jiangnan University,2018[嵇豪.Candida glycerinogenes HOG应答途径及渗透压耐受机制研究.博士学位论文.无锡:江南大学,2018]
19 Rodríguez-Pe?a J M,García R,Nombela C,et al.The high-osmolarity glycerol(HOG)and cell wall integrity(CWI)signalling pathways interplay:A yeast dialogue between MAPK routes.Yeast,2010,27:495-502
20 Cheetham J,Smith D A,da Silva Dantas A,et al.A single MAPKKK regulates the Hog1 MAPK pathway in the pathogenic fungus Candida albicans.Mol Biol Cell,2007,18:4603-4614
21 Furukawa K,Hoshi Y,Maeda T,et al.Aspergillus nidulans HOG pathway is activated only by two-component signalling pathway in response to osmotic stress.Mol Microbiol,2010,56:1246-1261
22 El-Mowafy M,Bahgat M M,Bilitewski U.Deletion of the HAMP domains from the histidine kinase CaNik1p of Candida albicans or treatment with fungicides activates the MAP kinase Hog1p in S.cerevisiae transformants.BMC Microbiol,2013,13:209
23 Hagiwara D,Suzuki S,Kamei K,et al.The role of AtfA and HOG MAPK pathway in stress tolerance in conidia of Aspergillus fumigatus.Fungal Genets Biol,2014,73:138-149
24 Posas F,Wurgler-Murphy S M,Maeda T,et al.Yeast HOG1 MAP kinase cascade is regulated by a multistep phosphorelay mechanism in the SLN1-YPD1-SSK1“two-component”osmosensor.Cell,1996,86:865-875
25 Eisman B,Alonso-Monge R,Román E,et al.The Cek1 and Hog1 mitogen-activated protein kinases play complementary roles in cell wall biogenesis and chlamydospore formation in the fungal pathogen Candida albicans.Eukaryotic Cell,2006,5:347-358
26 Azad G K,Singh V,Thakare M J,et al.Mitogen-activated protein kinase Hog1 is activated in response to curcumin exposure in the budding yeast Saccharomyces cerevisiae.BMC Microbiol,2014,14:317
27 Lawrence C L,Botting C H,Antrobus R,et al.Evidence of a new role for the high-osmolarity glycerol mitogen-activated protein kinase pathway in yeast:Regulating adaptation to citric acid stress.Mol Cell Biol,2004,24:3307-3323
28 Wang H,He Z,Luo L,et al.An aldo-keto reductase,Bbakr1,is involved in stress response and detoxification of heavy metal chromium but not required for virulence in the insect fungal pathogen,Beauveria bassiana.Fungal Genets Biol,2018,111:7-15
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