藏山羊与藏绵羊肺组织结构的对比研究
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摘要
为了探讨藏山羊(Capra hircus)与藏绵羊(Ovis aries)在高原低氧环境中肺组织结构的差异,采用Gomori醛品红染色及H.E染色对藏山羊和藏绵羊肺组织进行对比研究。结果表明,藏山羊与藏绵羊肺被膜厚度无显著差异(P> 0.05),但肺被膜中弹力纤维藏山羊显著多于藏绵羊(P <0.05)。肺泡面积藏山羊与藏绵羊无显著差异(P> 0.05),但肺泡隔宽度和肺泡隔中毛细血管的数量藏山羊显著高于藏绵羊(P <0.05)。肺细支气管黏膜皱襞厚度藏山羊与藏绵羊无显著差异(P> 0.05),但细支气管平滑肌厚度藏山羊显著高于藏绵羊,细支气管黏膜上皮1mm~2中杯状细胞数量藏山羊显著高于藏绵羊(P<0.05)。外径小于100μm的肺微动脉中,藏山羊血管平滑肌占外径百分比显著高于藏绵羊(P <0.05),而当外径大于100μm时,两者间差异不显著(P> 0.05)。
The Gomori and H.E staining were used to explore the difference in lung microstructure between Tibetan goat(Capra hircus) and Tibetan sheep(Ovis aries). The results showed that there was no significant difference in lung capsule thickness between these two species(P > 0.05), but the number of elastic fibers in Tibetan goat lung capsule was more than that in Tibetan sheep(P < 0.05)(Fig.1 and Table 1). There was no significant difference in alveolar area(P > 0.05), but the alveolar septum of Tibetan goat was significantly wider with much more capillaries than that of Tibetan sheep(P < 0.05)(Table 2). There was no significant difference in the thickness of bronchioles plica, but the bronchiole smooth muscle of Tibetan goat was significantly thicker than that of Tibetan sheep(P < 0.05)(Fig.2 and Table 3). The average number of goblet cells in the bronchioles mucosa epithelium of Tibetan goat was significantly more than that in Tibetan sheep(P < 0.05)(Table 3). In addition, the percentage of vascular smooth muscle in the pulmonary arteriole with diameter less than 100 μm was significantly higher in Tibetan goat than that in Tibetan sheep(P < 0.05), while the difference was not significant when the pulmonary arteriole diameter was greater than 100 μm(P > 0.05)( Table 4).
The Gomori and H.E staining were used to explore the difference in lung microstructure between Tibetan goat(Capra hircus) and Tibetan sheep(Ovis aries). The results showed that there was no significant difference in lung capsule thickness between these two species(P > 0.05), but the number of elastic fibers in Tibetan goat lung capsule was more than that in Tibetan sheep(P < 0.05)(Fig.1 and Table 1). There was no significant difference in alveolar area(P > 0.05), but the alveolar septum of Tibetan goat was significantly wider with much more capillaries than that of Tibetan sheep(P < 0.05)(Table 2). There was no significant difference in the thickness of bronchioles plica, but the bronchiole smooth muscle of Tibetan goat was significantly thicker than that of Tibetan sheep(P < 0.05)(Fig.2 and Table 3). The average number of goblet cells in the bronchioles mucosa epithelium of Tibetan goat was significantly more than that in Tibetan sheep(P < 0.05)(Table 3). In addition, the percentage of vascular smooth muscle in the pulmonary arteriole with diameter less than 100 μm was significantly higher in Tibetan goat than that in Tibetan sheep(P < 0.05), while the difference was not significant when the pulmonary arteriole diameter was greater than 100 μm(P > 0.05)( Table 4).
引文
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王晓亮.2013.藏羊和滩羊支气管树支气管动脉铸型方法的建立及其整体结构研究.兰州:甘肃农业大学博士学位论文.
魏青.2007.不同发育阶段高原牦牛和平原黄牛肺泡显微结构的研究.西宁:青海大学博士学位论文.
杨琨,余四九,何俊峰,等.2012.牦牛胎儿肺脏发育的形态学研究.兽类学报,32(4):346-355.
俞红贤.1999.藏羊肺组织形态测量指标及其与高原低氧的关系.中国兽医科技,29(7):15-16.
周大鹏,刘建国,王芳,等.2009.藏獒肺组织对高原低氧环境的适应特性.甘肃农业大学学报,44(4):25-28.
Deng J,Feng J,Li L,et al.2018.Polymorphisms,differentiation,and phylogeny of 10 Tibetan goat populations inferred from mitochondrial.D-loop sequences.Mitochondrial DNA Part A,29(3):439-445
Duffin J.2014.The fast exercise drive to breathe.The Journal of Physiology,592(3):445-451.
Marfatia S,Donahoe P K,Hendren W H.1975.Effect of dry and humidified gases on the respiratory epithelium in rabbits.Journal of Pediatric Surgery,10(5):583-592.
Mesit J,Guha R K,Furlong W P,et al.2011.Simulation of Lung Respiration Function Using Soft Body Model.2010 Fourth UKSim European Symposium on Computer Modeling and Simulation in Pisa,Italy.IEEE.
Mishra N C,Rirsimaah J,Langley R J,et al.2008.Nicotine primarily suppresses lung Th2 but not goblet cell and muscle cell responses to allergens.Journal of Immunology,180(11):7655-7663.
Moore C,Horton E,Benseler N,et al.2018.The effects of 100%oxygen on breathing pattern are not limited to young children.European Respiratory Journal,51(1):170-2055.
Singh K,Mariappa D.1982.Histological studies of the conducting and the respiratory divisions of the buffalo lung.Indian Veterinary Journal,58(2):99-183.
白玛央宗,商鹏,刘金凤.2012.等藏猪肺组织与高原低氧适应性的初步研究.湖北农业科学,51(13):2776-2779.
戴芳,王丽,王涛,等.2013.实验动物呼吸系统主要器官比较组织学研究.中国比较医学杂志,23(4):10-14.
何俊峰,余四九,崔燕.2009.不同年龄高原牦牛肺脏的组织结构特征.畜牧兽医学报,40(5):748-755.
李梦真.2017.青藏高原动物对环境的适应机制研究进展.青海科技,24(3):56-59.
马纯,何灵杰,杨孔.2007.藏鸡对高原环境的适应.四川动物,26(4):961-963.
马兴斌,崔燕,何俊峰,等.2011.成年牦牛支气管动脉的组织结构观察.中国兽医科学,41(3):303-307.
谭秀娟,何庆.2018.高原肺动脉高压发病机制研究进展.心血管病学进展,39(4):674-677.
王晓亮.2013.藏羊和滩羊支气管树支气管动脉铸型方法的建立及其整体结构研究.兰州:甘肃农业大学博士学位论文.
魏青.2007.不同发育阶段高原牦牛和平原黄牛肺泡显微结构的研究.西宁:青海大学博士学位论文.
杨琨,余四九,何俊峰,等.2012.牦牛胎儿肺脏发育的形态学研究.兽类学报,32(4):346-355.
俞红贤.1999.藏羊肺组织形态测量指标及其与高原低氧的关系.中国兽医科技,29(7):15-16.
周大鹏,刘建国,王芳,等.2009.藏獒肺组织对高原低氧环境的适应特性.甘肃农业大学学报,44(4):25-28.