基于非线性方法的食品着色剂光谱的研究
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摘要
食品着色剂大多含有对人体有害的物质,需要严格控制其用量。利用吸收光谱法和荧光光谱法测量出了五种常见的食品着色剂亮蓝、胭脂红、苋菜红、柠檬黄、日落黄的吸收光谱和荧光光谱。当溶液浓度较小时,对五种着色剂进行了光谱强度和溶液浓度的线性拟合,拟合得到的线性区间非常小,利用线性关系对溶液浓度进行定标存在较大的局限性,在高浓度时必须进行稀释。利用非线性拟合的方法在较大的浓度范围内探究了五种着色剂的光谱强度与溶液浓度的关系,在此基础上,分别用线性定标法(先稀释)和非线性定标法对一种饮料中的着色剂含量进行了测量,并对两种方法的测量结果进行了比较,为扩大溶液的定标范围提供了一种参考方法和实验依据。
Food colorants mostly contain substances harmful to human body, so their dosage should be strictly controlled. Absorption and fluorescence spectra of five common food colorants bright blue, carmine, amaranth, lemon yellow and sunset yellow were measured by absorption spectroscopy and fluorescence spectroscopy. When the concentration of solution is small, the linear fitting of spectral intensity and solution concentration of five colorants is carried out. The linear interval obtained by fitting is very small. There are great limitations in calibrating solution concentration by using linear relationship, and dilution must be carried out at high concentration. The relationship between the spectral intensity of five colorants and the concentration of solution was studied in a large concentration range by using the method of non-linear fitting, on this basis, using method of linear scaling(dilution) and nonlinear calibration method for a drink of colorants content were measured, and the measuring results of two methods are compared, to expand the selection range of solution provides a reference method and experimental basis.
Food colorants mostly contain substances harmful to human body, so their dosage should be strictly controlled. Absorption and fluorescence spectra of five common food colorants bright blue, carmine, amaranth, lemon yellow and sunset yellow were measured by absorption spectroscopy and fluorescence spectroscopy. When the concentration of solution is small, the linear fitting of spectral intensity and solution concentration of five colorants is carried out. The linear interval obtained by fitting is very small. There are great limitations in calibrating solution concentration by using linear relationship, and dilution must be carried out at high concentration. The relationship between the spectral intensity of five colorants and the concentration of solution was studied in a large concentration range by using the method of non-linear fitting, on this basis, using method of linear scaling(dilution) and nonlinear calibration method for a drink of colorants content were measured, and the measuring results of two methods are compared, to expand the selection range of solution provides a reference method and experimental basis.
引文
[1] 张卉.高效液相色谱法同时测定饮料中6种合成着色剂[J].现代食品,2018,2(025):81—84.Zhang Hui.Simultaneous determination of six synthetic colorants in beverages by high performance liquid chromatography[J].Modern Food,2018,2(025):81—84.
[2] 丁明珍,于海燕,王正堂.荧光光谱法测定饮料中的苋菜红与亮蓝[J].理化检验-化学分册工作简报,2015,51(10):1393—1395.Ding Mingzhen,Yu Haiyan,Wang Zhengtang.Determination of amaranth red and bright blue in beverages by fluorescence spectrometry[J].Briefing on the Work of Physical and Chemical Test-chemical Classification,2015,51(10):1393—1395.
[3] 王俊,陈国庆,魏柏林,等.基于BP神经网络的日落黄溶液荧光光谱的研究[J].光学技术,2009,35(6):868—871.Wang Jun,Chen Guoqing,Wei Bolin,et al.Study on fluorescence spectra of sunset yellow solution based on BP neural network[J].Optical Technique,2009,35(6):868—871.
[4] 郑新华,王乐,韩焕美,等.液相色谱串联质谱法检测食品中4种人工合成着色剂[J].食品安全质量检测学报,2016,7(4):1413—1450.Zheng Xinhua,Wang Le,Han Huanmei,et al.Determination of four synthetic colorants in food by liquid chromatography-tandem mass spectrometry[J].Journal of Food Safety and quality Inspection,2016,7(4):1413—1450.
[5] 肖海波,徐亚新.食品添加人工色素的危害性分析[J].中外医学研究,2009,7(6):124.Xiao Haibo,Xu Yaxin.Hazard analysis of artificial pigments added to food [J].Chinese and foreign medical research,2009,7 (6):124.
[6] 李瑾瑾.食品中合成着色剂分析方法研究[D].南昌大学,2011.Li Jinjin.Study on analytical methods of synthetic colorants in food [D].Nanchang University,2011.
[7] Yoshioka N,Ichihashi K.Determination of 40 synthetic food colors in drinks and candies by high-performance liquid chromatography using a short column with photodiode array detection[J].Talanta,2008,74:1408—1413.
[8] 张彦青.荧光光谱法测定食品中的苋菜红含量[J].山东化工,2017,46(7):124—127.Zhang Yanqing.Determination of amaranth in food by fluorescence spectrometry [J].Shandong Chemical Industry,2017,46(7):124—127.
[9] 史爱敏.常用食品合成色素胭脂红和苋菜红的荧光光谱特性研究[D].江南大学,2008.Shi Aimin.Study on the fluorescence spectroscopic characteristics of synthetic colors carmine and amaranth in common foods[D].Jiangnan University,2008.
[10] Frédéric Martin,Jean-Marie Oberson ,Marco Meschiari,et al.Determination of 18 water-soluble artificial dyes by LC-MS in selected matrices[J].Food Chemistry,2016,197:1249—1255.
[11] Badal Kumar Mandal,Siva Mathiyalagan,Ramesh Dalavai,et al.Simultaneous identification of synthetic and natural dyes in different food samples by UPLC-MS[J].IOP Conference Series:Materials Science and Engineering,2017,263(2):1—8.
[12] 张秋菊,曹林波,王硕,等.绿色高效液相色谱法测定饮料4种合成着色剂[J].中国卫生检验杂志,2018,28(7):785—789.Zhang Qiuju,Cao Linbo,Wang Shuo,et al.Determination of four synthetic colorants in beverages by green high performance liquid chromatography [J].Chinese Journal of Health Inspection,2018,28 (7):785—789.
[13] 吴红静,王维亚,叶维佳,等.软饮料中人工合成色素快速测定试纸条的研制[J].食品科学,2018,39(4):290—295.Wu Hongjing,Wang Wei,Ye Weijia,et al.Development of test strip for rapid determination of synthetic pigments in soft drinks [J].Food Science,2018,39(4):290—295.
[14] 李必斌,张海霞,潘连富.紫外一可见吸收光谱法定性定量测定食用合成色素[J].中国卫生检验杂志,2001,11(1):58—60.Li Bibin,Zhang Haixia,Pan Lianfu.Qualitative and quantitative determination of food synthetic pigments by UV-Vis absorption spectrometry [J].Chinese Journal of Health Inspection,2001,11(1):58—60.
[15] 王书涛,张彩霞,张强,等.基于改进的最小二乘支持向量机与荧光光谱法检测山梨酸钾[J].光学技术,2018,44(2):188—193.Wang Shutao,Zhang Caixia,Zhang Qiang,et al.Improved least squares support vector machine.determination of potassium sorbate by fluorescence spectrometry [J].Optical Technology,2018,44(2):188—193.
[16] 赵建林.光学[M] .北京:高等教育出版社,2006.Zhao Jianlin.Optical[M].Beijing:Higher Education Press,2006.
[17] 王颖,齐海燕,韩爽.谱学原理及应用[M].哈尔滨:哈尔滨工业大学出版社,2015.Wang Ying,Qi Haiyan,Han Shuang.Principles and applications of spectroscopy [M].Harbin:Harbin University of Technology Press,2015.
[18] 逯美红,雷海英,王志军,等.几种人工合成色素的荧光光谱与拉曼光谱研究[J].量子电子学报,2014,31(1):12—17.Lu Meihong,Lei Haiying,Wang Zhijun,et al.Studies on fluorescence and Raman spectra of several synthetic pigments [J].Journal of Quantum Electronics,2014,31(1):12—17.
[2] 丁明珍,于海燕,王正堂.荧光光谱法测定饮料中的苋菜红与亮蓝[J].理化检验-化学分册工作简报,2015,51(10):1393—1395.Ding Mingzhen,Yu Haiyan,Wang Zhengtang.Determination of amaranth red and bright blue in beverages by fluorescence spectrometry[J].Briefing on the Work of Physical and Chemical Test-chemical Classification,2015,51(10):1393—1395.
[3] 王俊,陈国庆,魏柏林,等.基于BP神经网络的日落黄溶液荧光光谱的研究[J].光学技术,2009,35(6):868—871.Wang Jun,Chen Guoqing,Wei Bolin,et al.Study on fluorescence spectra of sunset yellow solution based on BP neural network[J].Optical Technique,2009,35(6):868—871.
[4] 郑新华,王乐,韩焕美,等.液相色谱串联质谱法检测食品中4种人工合成着色剂[J].食品安全质量检测学报,2016,7(4):1413—1450.Zheng Xinhua,Wang Le,Han Huanmei,et al.Determination of four synthetic colorants in food by liquid chromatography-tandem mass spectrometry[J].Journal of Food Safety and quality Inspection,2016,7(4):1413—1450.
[5] 肖海波,徐亚新.食品添加人工色素的危害性分析[J].中外医学研究,2009,7(6):124.Xiao Haibo,Xu Yaxin.Hazard analysis of artificial pigments added to food [J].Chinese and foreign medical research,2009,7 (6):124.
[6] 李瑾瑾.食品中合成着色剂分析方法研究[D].南昌大学,2011.Li Jinjin.Study on analytical methods of synthetic colorants in food [D].Nanchang University,2011.
[7] Yoshioka N,Ichihashi K.Determination of 40 synthetic food colors in drinks and candies by high-performance liquid chromatography using a short column with photodiode array detection[J].Talanta,2008,74:1408—1413.
[8] 张彦青.荧光光谱法测定食品中的苋菜红含量[J].山东化工,2017,46(7):124—127.Zhang Yanqing.Determination of amaranth in food by fluorescence spectrometry [J].Shandong Chemical Industry,2017,46(7):124—127.
[9] 史爱敏.常用食品合成色素胭脂红和苋菜红的荧光光谱特性研究[D].江南大学,2008.Shi Aimin.Study on the fluorescence spectroscopic characteristics of synthetic colors carmine and amaranth in common foods[D].Jiangnan University,2008.
[10] Frédéric Martin,Jean-Marie Oberson ,Marco Meschiari,et al.Determination of 18 water-soluble artificial dyes by LC-MS in selected matrices[J].Food Chemistry,2016,197:1249—1255.
[11] Badal Kumar Mandal,Siva Mathiyalagan,Ramesh Dalavai,et al.Simultaneous identification of synthetic and natural dyes in different food samples by UPLC-MS[J].IOP Conference Series:Materials Science and Engineering,2017,263(2):1—8.
[12] 张秋菊,曹林波,王硕,等.绿色高效液相色谱法测定饮料4种合成着色剂[J].中国卫生检验杂志,2018,28(7):785—789.Zhang Qiuju,Cao Linbo,Wang Shuo,et al.Determination of four synthetic colorants in beverages by green high performance liquid chromatography [J].Chinese Journal of Health Inspection,2018,28 (7):785—789.
[13] 吴红静,王维亚,叶维佳,等.软饮料中人工合成色素快速测定试纸条的研制[J].食品科学,2018,39(4):290—295.Wu Hongjing,Wang Wei,Ye Weijia,et al.Development of test strip for rapid determination of synthetic pigments in soft drinks [J].Food Science,2018,39(4):290—295.
[14] 李必斌,张海霞,潘连富.紫外一可见吸收光谱法定性定量测定食用合成色素[J].中国卫生检验杂志,2001,11(1):58—60.Li Bibin,Zhang Haixia,Pan Lianfu.Qualitative and quantitative determination of food synthetic pigments by UV-Vis absorption spectrometry [J].Chinese Journal of Health Inspection,2001,11(1):58—60.
[15] 王书涛,张彩霞,张强,等.基于改进的最小二乘支持向量机与荧光光谱法检测山梨酸钾[J].光学技术,2018,44(2):188—193.Wang Shutao,Zhang Caixia,Zhang Qiang,et al.Improved least squares support vector machine.determination of potassium sorbate by fluorescence spectrometry [J].Optical Technology,2018,44(2):188—193.
[16] 赵建林.光学[M] .北京:高等教育出版社,2006.Zhao Jianlin.Optical[M].Beijing:Higher Education Press,2006.
[17] 王颖,齐海燕,韩爽.谱学原理及应用[M].哈尔滨:哈尔滨工业大学出版社,2015.Wang Ying,Qi Haiyan,Han Shuang.Principles and applications of spectroscopy [M].Harbin:Harbin University of Technology Press,2015.
[18] 逯美红,雷海英,王志军,等.几种人工合成色素的荧光光谱与拉曼光谱研究[J].量子电子学报,2014,31(1):12—17.Lu Meihong,Lei Haiying,Wang Zhijun,et al.Studies on fluorescence and Raman spectra of several synthetic pigments [J].Journal of Quantum Electronics,2014,31(1):12—17.
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