离体蛙心灌流实验中4种蛙心插管方法的比较
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摘要
目的比较并评价离体蛙心灌流实验中4种插管方法的效果。方法分别采用斯氏法、八木氏法、右主动脉插管法、后腔静脉+左主动脉插管法制备离体蛙心标本,分析4种插管方法在蛙心插管所需时间、心脏离体时间、插管成功率、存活率、平均存活时间及凝血率方面的差异。结果与传统插管方法即斯氏法相比,两种双插管法在插管成功率、标本存活率及标本存活时间上显著提高,凝血率显著降低,差异有统计学意义(P<0.05);但其插管及心脏离体耗时显著延长,差异有统计学意义(P<0.05)。与斯氏法比较,右主动脉插管法在插管成功率、标本存活率及标本存活时间上显著提高,凝血率显著降低,插管耗时也显著降低,差异有统计学意义(P<0.05);但其心脏离体时间与斯氏法相比差异无统计学意义(P>0.05)。与两种双插管法相比,右主动脉插管法插管成功率、插管时间及蛙心离体时间显著降低,凝血率升高,差异有统计学意义(P<0.05);但标本存活时间及标本存活率差异无统计学意义(P>0.05)。结论综合评价,在离体蛙心灌流实验中右主动脉插管法有较大优势。
Objective To compare and evaluate the effectiveness of four methods of intubation in isolated frog heart perfusion. Methods Isolated frog heart specime were prepared by Straub method, BAMU-HARTUNG'S method, right aortic intubation and posterior vena cava+left aorta intubation method, to analyze the differences of four methods in the time of cannulation, time of heart ex vivo, successful rate, survival rate, heart survival time and rate of coagulation. Results Compared with the traditional intubation-Straub method, the two kinds of double-cannula method were increased in the successful rate, survival rate and heart survival time, decreased in the rate of coagulation, and there were significant difference(P<0.05),but increased in the time of cannulation and isolated heart, and there were significant difference(P<0.05). Compared with Straub method, right aortic intubation was increased in the successful rate, survival rate and heart survival time, decreased in coagulation rate and the time of cannulation, and there were significant difference(P<0.05).But the time of isolated heart was no significant difference( P > 0. 05). Compared with the two kinds of double-cannula method,right aortic intubation was decreased in successful rate,the time of cannulation and isolated heart,increased in coagulation rate,and there were significant difference( P < 0. 05),but heart survival time and survival rate had no significant difference( P > 0. 05). Conclusion The comprehensive evaluation showed that the right aortic intubation method had a great advantage in the isolated frog heart perfusion experiment.
Objective To compare and evaluate the effectiveness of four methods of intubation in isolated frog heart perfusion. Methods Isolated frog heart specime were prepared by Straub method, BAMU-HARTUNG'S method, right aortic intubation and posterior vena cava+left aorta intubation method, to analyze the differences of four methods in the time of cannulation, time of heart ex vivo, successful rate, survival rate, heart survival time and rate of coagulation. Results Compared with the traditional intubation-Straub method, the two kinds of double-cannula method were increased in the successful rate, survival rate and heart survival time, decreased in the rate of coagulation, and there were significant difference(P<0.05),but increased in the time of cannulation and isolated heart, and there were significant difference(P<0.05). Compared with Straub method, right aortic intubation was increased in the successful rate, survival rate and heart survival time, decreased in coagulation rate and the time of cannulation, and there were significant difference(P<0.05).But the time of isolated heart was no significant difference( P > 0. 05). Compared with the two kinds of double-cannula method,right aortic intubation was decreased in successful rate,the time of cannulation and isolated heart,increased in coagulation rate,and there were significant difference( P < 0. 05),but heart survival time and survival rate had no significant difference( P > 0. 05). Conclusion The comprehensive evaluation showed that the right aortic intubation method had a great advantage in the isolated frog heart perfusion experiment.
引文
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[2] 陈珍,赵有亮.生附子和制附子对离体蛙心的强心作用[J].科技视界,2013(36):390.DOI:10.19694/j.cnki.issn2095-2457.2013.36.321.
[3] 李旭伟,姜黛雪,王欢,等.破格救心汤对离体蟾蜍心脏功能的影响[J].中国民族民间医药,2013,22(6):29-30.DOI:10.3969/j.issn.1007-8517.2013.06.018.
[4] 蒿慧文,肖雪,张扬媚,等.提高离体蛙心灌流实验成功率的方法研究[J].实验技术与管理,2012,29(9):67-68,78.DOI:10.16791/j.cnki.sjg.2012.09.019.
[5] 林平,潘慧,苏付荣,等.离体蛙心灌流实验方法的比较研究[J].中国病理生理杂志,2005,21(7):1455-1456.DOI:10.3321/j.issn:1000-4718.2005.07.047.
[6] 孙素珍,王玉中.川芎水煎液对离体蟾蜍心功能的影响[J].四川生理科学杂志,2008(1):12-13.DOI:10.3969/j.issn.1671-3885.2008.01.006.
[7] 王小君,孙娜,王银环,等.蛙心灌流实验中插管方法的改进[J].华北煤炭医学院学报,2010,12(4):457-458.DOI:10.19539/j.cnki.2095-2694.2010.04.003.
[8] 胡文嫣,南旭阳,陈玥,等.小型蛙离体心脏灌流实验方法的研究[J].河南科学,2012,30(2):196-199.DOI:10.13537/j.issn.1004-3918.2012.02.030
[9] 王玉中,孙素珍,蔡智慧,等.斯氏法、八木氏法制备离体蛙心灌流标本比较[J].卫生职业教育,2008,26(1):116-117.DOI:10.3969/j.issn.1671-1246.2008.01.077.
[10] 邓文娟,李锦平.离体蛙心灌流实验方法的比较[J].继续医学教育,2018,32(9):55-56.DOI:10.3969/j.issn.1004-6763.2018.09.032.