WU多瘤病毒所致小儿呼吸道感染的临床特征研究
|
摘要
目的探讨WU多瘤病毒(WU polyomavirus,WUPyV)所致小儿呼吸道感染的临床特征。
材料与方法
材料:收集2008年1月至2010年9月汕头大学医学院第二附属医院儿科1701例因呼吸道感染住院患儿咽拭子,并分别收集WUPyV感染和RSV感染患儿年龄、性别、症状、体征等临床资料。
方法:1、应用多重PCR技术,检测WU多瘤病毒(WUPyV)、呼吸道合胞病毒(RSV)、人博卡病毒(HBoV)、腺病毒(ADV)、鼻病毒(RhV)、流感病毒A、B型(FluA、FluB)、副流感病毒1、3型(PIV1、PIV3)、人类偏肺病毒(hMPV)10种(型)病毒。
2、对WUPyV阳性病例的临床资料进行分析,并与RSV阳性病例的临床资料进行分析比较。
结果
1、1701例患儿标本中检测阳性1215例(71.4%),其中WUPyV阳性46例(2.7%),RSV阳性365例(21.5%);两者单纯感染分别为22例(47.8%)和88例(24.1%)。
2、WUPyV阳性检测率在春季呈现高峰,RSV则在冬季出现高峰;WUPyV感染的患儿的平均年龄为26.23月龄,较RSV感染的患儿大。
3、WUPyV感染患儿的临床特征与RSV感染相似,但咳嗽和喘息在RSV感染患儿中更为多见;两者在肺部体征、WBC计数、CRP值、住院天数无明显区别。WUPyV感染后所致的疾病主要是支气管肺炎,而RSV感染后所致的疾病主要是毛细支气管炎。
结论
1、小儿呼吸道感染部分与WUPyV感染有关。
2、WUPyV感染的临床特点是:以1—3岁年龄组儿童常见,在春季多见,其临床表现与普通的支气管肺炎和支气管炎类似,少见重症表现。
3、WUPyV合并其它病毒感染率高,常与RSV合并感染。
Objective To study the clinical features of respiratory infection caused by WU polyomavirus ( WUPyV) in children..
Material and methods
Material: From January 2008 to September 2010, throat swab samples were collected from1701 children who were hospitalized with respiratory tract infection in Second Affiliated Hospital of Shantou University Medical College, and the clinical datas about WUPyV-positive cases or WUPyV-positive cases were collected including age, gender, symptoms and signs.
Methods: 1、Multiplex PCR was applied to detect 10 kinds of viruses including WU Polyomavirus (WUPyV), respiratory syncytial virus (RSV), Boca virus (HBoV), adenovirus (ADV), rhinovirus (RhV), influenza virus A, B type (FluA, FluB), parainfluenza virus type 1,3 (PIV1, PIV3 ) and Human metapneumovirus (hMPV).
2、To compare the clinical datas of WUPyV-positive cases with that of WUPyV-positive cases.
Results
1、There were 1215 positive samples (71.4%) in 1701 specimens, with 46 WUPyV-positive cases (2.7%) and 365 RSV-positive cases (21.5%). There were 22 cases (47.8%) and 88 cases (24.1%) of simple infection for WUPyV and RSV.
2、The positive rate of WUPyV peaked in spring, while the positive rate of RSV reached its peak in winter; the average age of children with WUPyV infection was 26.23 months old, greater than that of children with RSV infection.
3、Clinical characteristics of children infected with WUPyV and RSV are similar, but Coughing, wheezing were more common in children with RSV infection; There was no significant difference in the aspects of signs in the lungs, WBC count, CRP value, length of stay and severity of disease for both virus infection. The disease caused by WUPyV infection is mainly bronchial pneumonia, while the disease caused by RSV infection is mainly capillary bronchitis.
Conclusions
1、Respiratory tract infections in children were partly caused by WUPyV infection;
2、WUPyV infection were more common in the 1-3 age group children; the positive rate of WUPyV peaked in spring,and its clinical manifestations are similar to that of normal bronchial pneumonia and bronchitis, but rare in serious cases.
3、WUPyV infection has a higher rate of co-infection with other viruses, especially with RSV.
材料与方法
材料:收集2008年1月至2010年9月汕头大学医学院第二附属医院儿科1701例因呼吸道感染住院患儿咽拭子,并分别收集WUPyV感染和RSV感染患儿年龄、性别、症状、体征等临床资料。
方法:1、应用多重PCR技术,检测WU多瘤病毒(WUPyV)、呼吸道合胞病毒(RSV)、人博卡病毒(HBoV)、腺病毒(ADV)、鼻病毒(RhV)、流感病毒A、B型(FluA、FluB)、副流感病毒1、3型(PIV1、PIV3)、人类偏肺病毒(hMPV)10种(型)病毒。
2、对WUPyV阳性病例的临床资料进行分析,并与RSV阳性病例的临床资料进行分析比较。
结果
1、1701例患儿标本中检测阳性1215例(71.4%),其中WUPyV阳性46例(2.7%),RSV阳性365例(21.5%);两者单纯感染分别为22例(47.8%)和88例(24.1%)。
2、WUPyV阳性检测率在春季呈现高峰,RSV则在冬季出现高峰;WUPyV感染的患儿的平均年龄为26.23月龄,较RSV感染的患儿大。
3、WUPyV感染患儿的临床特征与RSV感染相似,但咳嗽和喘息在RSV感染患儿中更为多见;两者在肺部体征、WBC计数、CRP值、住院天数无明显区别。WUPyV感染后所致的疾病主要是支气管肺炎,而RSV感染后所致的疾病主要是毛细支气管炎。
结论
1、小儿呼吸道感染部分与WUPyV感染有关。
2、WUPyV感染的临床特点是:以1—3岁年龄组儿童常见,在春季多见,其临床表现与普通的支气管肺炎和支气管炎类似,少见重症表现。
3、WUPyV合并其它病毒感染率高,常与RSV合并感染。
Objective To study the clinical features of respiratory infection caused by WU polyomavirus ( WUPyV) in children..
Material and methods
Material: From January 2008 to September 2010, throat swab samples were collected from1701 children who were hospitalized with respiratory tract infection in Second Affiliated Hospital of Shantou University Medical College, and the clinical datas about WUPyV-positive cases or WUPyV-positive cases were collected including age, gender, symptoms and signs.
Methods: 1、Multiplex PCR was applied to detect 10 kinds of viruses including WU Polyomavirus (WUPyV), respiratory syncytial virus (RSV), Boca virus (HBoV), adenovirus (ADV), rhinovirus (RhV), influenza virus A, B type (FluA, FluB), parainfluenza virus type 1,3 (PIV1, PIV3 ) and Human metapneumovirus (hMPV).
2、To compare the clinical datas of WUPyV-positive cases with that of WUPyV-positive cases.
Results
1、There were 1215 positive samples (71.4%) in 1701 specimens, with 46 WUPyV-positive cases (2.7%) and 365 RSV-positive cases (21.5%). There were 22 cases (47.8%) and 88 cases (24.1%) of simple infection for WUPyV and RSV.
2、The positive rate of WUPyV peaked in spring, while the positive rate of RSV reached its peak in winter; the average age of children with WUPyV infection was 26.23 months old, greater than that of children with RSV infection.
3、Clinical characteristics of children infected with WUPyV and RSV are similar, but Coughing, wheezing were more common in children with RSV infection; There was no significant difference in the aspects of signs in the lungs, WBC count, CRP value, length of stay and severity of disease for both virus infection. The disease caused by WUPyV infection is mainly bronchial pneumonia, while the disease caused by RSV infection is mainly capillary bronchitis.
Conclusions
1、Respiratory tract infections in children were partly caused by WUPyV infection;
2、WUPyV infection were more common in the 1-3 age group children; the positive rate of WUPyV peaked in spring,and its clinical manifestations are similar to that of normal bronchial pneumonia and bronchitis, but rare in serious cases.
3、WUPyV infection has a higher rate of co-infection with other viruses, especially with RSV.
引文
[1]. Shay D K, Holman R C, Newman R D, et al. Bronchiolitis-associated hospitalizations among US children,1980-1996. JAMA,1999, 282: 1440-1446.
[2]. Lowther SA,Shay DK, Holman RC, et al. Bronchiolitis-associated hospitalizations among American Indian and Alaska Native children. Pediatr Infect Dis J, 2000, 19: 11-17.
[3]. Van den Hoogen BG, de Jong JC, Groen J, et al. A newly discovered human pneumovirus isolated from young children with respiratory tract disease. Nat Med, 2001, 7 : 719-724.
[4]. Ksiazek TG, Erdman D, Goldsmith CS, et al. A novel coronavirus associated with severe acute respiratory syndrome. N Engl J Med, 2003, 348: 1953-1966.
[5]. Van der Hoek L, Pyrc K, Jebbink MF, et al. Identification of a new human coronanavirus. Nat Med, 2004,10: 368-373.
[6]. Woo PC, Lau SK, Chu CM, et al. Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia. J Virol, 2005, 79:884-895.
[7]. Allander T, Tammi MT, Eriksson M, et al. Cloning of a human parvovirus by molecular screening of respiratory tract samples . Proc Natl Acad Sci USA, 2005, 102 : 12891-12896.
[8]. Renwick N, Schweiger B, Kapoor V, et al. A recently identified rhinovirus genotype is associated with severe respiratory-tract infection in children in Germany. J Infect Dis, 2007,196: 1754-1760.
[9]. Allander T, Andreasson K, Gupta S, et al. Identification of a third human polyomavirus. J Virol, 2007, 81: 4130-4136.
[10]. Gaynor AM, Nissen MD, Whiley DM, et al. Identification of a novel polyomavirus from patients with acute respiratory tract infections . PLoS Pathol, 2007, 3: e64.
[11]. Le BM, Demertzis LM, Wu G, et al. Clinical and epidemiologic characterization of WU polymavirus Infection, St. Louis, Missouri. Emerg Infect Dis, 2007, 13: 1936-1938.
[12]Bialasiewicz S, Whiley DM, Lambert SB, et al. Presence of the newly discovered human polyomavirus KI and WU in Australian patients with acute respiratory tract infection. J Clin Virol, 2008, 41 :63-68..
[13]Neske F, Blessing K, Ullrich F, et al. WU Polyomavirus infection in children, Germany.Emerg Infect Dis, 2008, 14 :680-681.
[14]Foulongne V, Brieu N, Jeziorski E, et al. KI and WU polyomavirus in children, France. Emerg Infect Dis, 2008, 14:523-525.
[15]5van der Zalm MM, Rossen JW, van Ewijk BE, et al. Prevalence and pathogenicity of WU and KI polyomavirus in children, the Netherlands. Emerg Infect Dis, 2008, 14:1787-1789.
[16]Yuan XH, Jin Y, Xie ZP, et al .Prevalence of human KI and WU polymavirus in children with acute respiratory tract infection in China. J Clin Microbiol, 2008, 46: 3522-3525.
[17]Abed Y, Wang D, Boivin G. WU Polyomavirus in children, Canada. Emerg Infect Dis, 2007, 13:1939-1941.
[18]Han TH, Chung JY, Koo JW, et al. WU Polyomavirus in children with acute lower respiratory tract infections, South Korea. Emerg Infect Dis, 2007, 13: 1766-1768.
[19]Gardner SD, Field AM, Coleman DV, et al. New human papovavirus(B.K.) isolated from urine after renal transplantation. Lancet, 1971, 1:1253-1257.
[20]Padgett BL, Walker DL, ZuRhein GM, et al.Cultivation of papova-like virus from human brain with progressivemultifocal leucoencephalopathy. Lancet, 1971, 1: 1257-1260.
[1] Le BM, Demertzis LM, Wu G, et al. Clinical and epidemiologic characterization of WU polymavirus Infection, St. Louis, Missouri. Emerg Infect Dis, 2007, 13: 1936-1938.
[2]Gaynor AM, Nissen MD, Whiley DM, et al. Identification of a novel polyomavirus from patients with acute respiratory tract infections . PLoS Pathol, 2007, 3: e64.
[3]Bialasiewicz S, Whiley DM, Lambert SB, et al. Presence of the newly discovered human polyomavirus KI and WU in Australian patients with acute respiratory tract infection. J Clin Virol, 2008, 41 :63-68.
[4]Abed Y, Wang D, Boivin G. WU Polyomavirus in children, Canada. Emerg Infect Dis, 2007, 13:1939-1941.
[5] Han TH, Chung JY, Koo JW, et al. WU Polyomavirus in children with acute lower respiratory tract infections, South Korea. Emerg Infect Dis, 2007, 13: 1766-1768.
[6] Neske F, Blessing K, Ullrich F, et al. WU Polyomavirus infection in children, Germany. Emerg Infect Dis, 2008, 14 :680-681.
[7] van der Zalm MM, Rossen JW, van Ewijk BE, et al. Prevalence and pathogenicity of WU and KI polyomavirus in children, the Netherlands. Emerg Infect Dis, 2008, 14:1787-1789.
[8] Foulongne V, Brieu N, Jeziorski E, et al. KI and WU polyomavirus in children, France. Emerg Infect Dis, 2008, 14:523-525.
[9]林峰,郑美云,李桦,等.WU多瘤病毒在中国浙江地区的发现与鉴定.病毒学报, 2008, 24:69-71.
[10]Zhao L, Qian Y, Zhu R et al. Indentification of WU polymavirus from pediatric patients with acute respiratory infections in Beijing, China. Arch Virol, 2010, 155:181-186. Epub 2009 Nov 28.
[11]贾雪,陆学东,王琼,等.广东地区首例WU多瘤病毒的检出与鉴定.中华检验医学杂志, 2009, 32: 1169-1170.
[12]庄婉莉,陆学东,林广裕,等. WU多瘤病毒在儿童急性呼吸道感染的检出及初步临床研究.中华儿科杂志, 2010, 48: 90-94.
[13]Yuan XH, Jin Y, Xie ZP, et al .Prevalence of human KI and WU polymavirus in children with acute respiratory tract infection in China. J Clin Microbiol, 2008, 46: 3522-3525.
[14]Boivin G,De Serres G,Cote S,et al Human metapneumovirus infections in hospitalized children[J].Emerg Infect Dis,2003,9:634-640.
[15]Perirs JS,Tang WH,Chan KH,et al Children with respiratory disease associated with metapneumovirus in Hong Kong[J].Emerg Infect Dis,2003,9:628-633.
[16]Zambon MC,Stockton JD,Clewley JP,et al.Contribution of influenza and respiratory syncytial virus to community cares of influenza like illness:all observational study[J].Lancet,2001;358(7254):1410-1416.
[17]Sonoda S,Get oh Y,Bann F,et al.Acute lower respiratory infections in hospitalized children over a 6 year period in Tokyo[J].Pediatr Int,1999;41(5):519-524.
[1] Shay D K, Holman R C , Newman R D , et al. Bronchiolitis-associated hospitalizations among US children ,1980-1996. JAMA ,1999 , 282: 1440-1446.
[2] Shay D K, Holman R C, Newman R C, et al. Bronchiolitis-associated hospitalizations among American Indian and Alaska Native children. Pediatr Infect Dis J, 2000, 19: 11-17.
[3] Van den Hoogen B G, de Jong J C , Groen J , et al. A newly discovered human pneumovirus isolated from young children with respiratory tract disease. Nat Med , 2001 , 7 : 719-724.
[4] Ksiazek T G,Erdman D,Goldsmith C S,et al.A novel coronavirus associated with severe acute respiratory syndrome.N Engl J Med,2003,348:1953-1966.
[5] Van der Hoek L,Pyrc K,Jebbink M F, et al.Identification of a new human coronanavirus.Nat Med,2004,10:368-373.
[6] Woo P C,Lau S K,Chu C M,et al.Characterization and complete genome sequence of a novel coronavirus,coronavirus HKU1,from patients with pneumonia.J Virol,2005,79:884-895.
[7] Allander T , Tammi M T , Eriksson M , et al. Cloning of a human parvovirus by molecular screening of respiratory tract samples . Proc Natl Acad Sci USA , 2005 , 102 :12891-12896.
[8] Renwick N,Schweiger B,Kapoor V,et al.A recently identified rhinovirus genotype is associated with severe respiratory-tract infection in children in Germany. J Infect Dis,2007 ,196;1754-1760.
[9] Allander T,Andreasson K,Gupta S,et al.Identification of a third human polyomavirus.J Virol,2007,81:4130-4136.
[10] Gaynor A M , Nissen M D , Whiley D M , et al. Identification of a novel polyomavirus from patients with acute respiratory tract infections . PLoS Pathol ,2007 , 3: e64.
[11] Bialasiewicz S, Whiley DM, Lambert SB, et al. Presence of the newly discovered human polyomavirus KI and WU in Australian patients with acute respiratory tractinfection. J Clin Virol, 2008, 41 :63-68.
[12] Han TH, Chung JY, Koo JW, et al. WU Polyomavirus in Children with Acute Lower Respiratory Tract Infections, South Korea. Emerg Infect Dis,2007,13: 1766-1768.
[13] Abed Y, Wang W, Boivin G. WU Polyomavirus in Children, Canada. Emerg Infect Dis, 2007, 13:1939-1941.
[14] Neske F, Blessing K, Ullrich F, et al. WU Polyomavirus Infection in Children, Germany. Emerg Infect Dis,2008,14 :680-681.
[15] Foulongne V, Brieu N, Jeziorski E, et al. KI and WU Polyomaviruses in Children, France. Emerg Infect Dis,2008, 14:523-525.
[16]林峰,郑美云,李桦,等.WU多瘤病毒在中国浙江地区的发现与鉴定.病毒学报,2008 ,24:69-71.
[17] Zhao LQ, Qian Y, Zhu RN,et al. Identification of WU polymavirus from pediatric patients with acute respiratory infections in Beijing,China.Original Article,2010,155:181-186.
[18]贾雪,陆学东,王琼,等.广东地区首例WU多瘤病毒的检出与鉴定.中华检验医学杂志,2009,32:1169-1170.
[19] Gardner SD, Field AM, Coleman DV, Hulme B. New human papovavirus(B.K.) isolated from urine after renal transplantation. Lancet,1971,1:1253–7.
[20] Padgett BL, Walker DL, Zurhein GM, Eckorade RJ, Dessel BH.Cultivation of papova-like virus from human brain with progressivemultifocal leucoencephalopathy. Lancet, 1971,1: 1257–60.
[21]庄婉莉,陆学东,林广裕,等.WU多瘤病毒在儿童急性呼吸道感染的检出及初步临床研究.中华儿科杂志,2010 ,48:90-94.
[22] Le BM, Demertzis LM, Wu G, et al. Clinical and Epidemiologic Characterization of WU Polyomavirus Infection, St. Louis, Missouri. Emerg Infect Dis , 2007 , 13: 1936–1938.
[23] Marieke M.van der Zalm,John W.A.Rossen,BartE.van Ewijk ,et al. Prevalence and Pathogenicity of WU and KI Polyomaviruses in Children,the Netherlands. Emerg Infect Dis,2008,14:1787-1789.
[24] Yuan X H, Jin Y, Xie Z P, et al .Prevalence of Human KI and WU Polyomaviruses inChildren with Acute Respiratory Tract Infection in China. J Clin Microbiol ,2008,46:3522-3525.
[25] Muhanmed Babakir-Mina,Massimo Ciccozzi,Elisabetta Trento, et al .KI and WU Polyomaviruses in Patients Infected with HIV-1,Italy. Emerg Infect Dis,2009 ,15:1323-1325.
[26] Ren LL, Gonzalez R, Xu XW, et al. WU Polyomavirus in Fecal Specimens of Children with Acute Gastroenteritis,China. Emerg Infect Dis, 2009,15:134-135.
[27] Payungporn S, Chieochansin T, Thongmee C, et al. Detection and discrimination of Wu/Ki polyomaviruses by real-time PCR with melting curve analysis.Virological Methods J,2008 ,153:70-73.
[28] Nguyen NL, Le BM, Wang D. Serologic Evidence of Frequent Hu,man Infection with WU and KI Polyomaviruses. Emerg Infect Dis,2009 ,15:1199-1205.
[2]. Lowther SA,Shay DK, Holman RC, et al. Bronchiolitis-associated hospitalizations among American Indian and Alaska Native children. Pediatr Infect Dis J, 2000, 19: 11-17.
[3]. Van den Hoogen BG, de Jong JC, Groen J, et al. A newly discovered human pneumovirus isolated from young children with respiratory tract disease. Nat Med, 2001, 7 : 719-724.
[4]. Ksiazek TG, Erdman D, Goldsmith CS, et al. A novel coronavirus associated with severe acute respiratory syndrome. N Engl J Med, 2003, 348: 1953-1966.
[5]. Van der Hoek L, Pyrc K, Jebbink MF, et al. Identification of a new human coronanavirus. Nat Med, 2004,10: 368-373.
[6]. Woo PC, Lau SK, Chu CM, et al. Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia. J Virol, 2005, 79:884-895.
[7]. Allander T, Tammi MT, Eriksson M, et al. Cloning of a human parvovirus by molecular screening of respiratory tract samples . Proc Natl Acad Sci USA, 2005, 102 : 12891-12896.
[8]. Renwick N, Schweiger B, Kapoor V, et al. A recently identified rhinovirus genotype is associated with severe respiratory-tract infection in children in Germany. J Infect Dis, 2007,196: 1754-1760.
[9]. Allander T, Andreasson K, Gupta S, et al. Identification of a third human polyomavirus. J Virol, 2007, 81: 4130-4136.
[10]. Gaynor AM, Nissen MD, Whiley DM, et al. Identification of a novel polyomavirus from patients with acute respiratory tract infections . PLoS Pathol, 2007, 3: e64.
[11]. Le BM, Demertzis LM, Wu G, et al. Clinical and epidemiologic characterization of WU polymavirus Infection, St. Louis, Missouri. Emerg Infect Dis, 2007, 13: 1936-1938.
[12]Bialasiewicz S, Whiley DM, Lambert SB, et al. Presence of the newly discovered human polyomavirus KI and WU in Australian patients with acute respiratory tract infection. J Clin Virol, 2008, 41 :63-68..
[13]Neske F, Blessing K, Ullrich F, et al. WU Polyomavirus infection in children, Germany.Emerg Infect Dis, 2008, 14 :680-681.
[14]Foulongne V, Brieu N, Jeziorski E, et al. KI and WU polyomavirus in children, France. Emerg Infect Dis, 2008, 14:523-525.
[15]5van der Zalm MM, Rossen JW, van Ewijk BE, et al. Prevalence and pathogenicity of WU and KI polyomavirus in children, the Netherlands. Emerg Infect Dis, 2008, 14:1787-1789.
[16]Yuan XH, Jin Y, Xie ZP, et al .Prevalence of human KI and WU polymavirus in children with acute respiratory tract infection in China. J Clin Microbiol, 2008, 46: 3522-3525.
[17]Abed Y, Wang D, Boivin G. WU Polyomavirus in children, Canada. Emerg Infect Dis, 2007, 13:1939-1941.
[18]Han TH, Chung JY, Koo JW, et al. WU Polyomavirus in children with acute lower respiratory tract infections, South Korea. Emerg Infect Dis, 2007, 13: 1766-1768.
[19]Gardner SD, Field AM, Coleman DV, et al. New human papovavirus(B.K.) isolated from urine after renal transplantation. Lancet, 1971, 1:1253-1257.
[20]Padgett BL, Walker DL, ZuRhein GM, et al.Cultivation of papova-like virus from human brain with progressivemultifocal leucoencephalopathy. Lancet, 1971, 1: 1257-1260.
[1] Le BM, Demertzis LM, Wu G, et al. Clinical and epidemiologic characterization of WU polymavirus Infection, St. Louis, Missouri. Emerg Infect Dis, 2007, 13: 1936-1938.
[2]Gaynor AM, Nissen MD, Whiley DM, et al. Identification of a novel polyomavirus from patients with acute respiratory tract infections . PLoS Pathol, 2007, 3: e64.
[3]Bialasiewicz S, Whiley DM, Lambert SB, et al. Presence of the newly discovered human polyomavirus KI and WU in Australian patients with acute respiratory tract infection. J Clin Virol, 2008, 41 :63-68.
[4]Abed Y, Wang D, Boivin G. WU Polyomavirus in children, Canada. Emerg Infect Dis, 2007, 13:1939-1941.
[5] Han TH, Chung JY, Koo JW, et al. WU Polyomavirus in children with acute lower respiratory tract infections, South Korea. Emerg Infect Dis, 2007, 13: 1766-1768.
[6] Neske F, Blessing K, Ullrich F, et al. WU Polyomavirus infection in children, Germany. Emerg Infect Dis, 2008, 14 :680-681.
[7] van der Zalm MM, Rossen JW, van Ewijk BE, et al. Prevalence and pathogenicity of WU and KI polyomavirus in children, the Netherlands. Emerg Infect Dis, 2008, 14:1787-1789.
[8] Foulongne V, Brieu N, Jeziorski E, et al. KI and WU polyomavirus in children, France. Emerg Infect Dis, 2008, 14:523-525.
[9]林峰,郑美云,李桦,等.WU多瘤病毒在中国浙江地区的发现与鉴定.病毒学报, 2008, 24:69-71.
[10]Zhao L, Qian Y, Zhu R et al. Indentification of WU polymavirus from pediatric patients with acute respiratory infections in Beijing, China. Arch Virol, 2010, 155:181-186. Epub 2009 Nov 28.
[11]贾雪,陆学东,王琼,等.广东地区首例WU多瘤病毒的检出与鉴定.中华检验医学杂志, 2009, 32: 1169-1170.
[12]庄婉莉,陆学东,林广裕,等. WU多瘤病毒在儿童急性呼吸道感染的检出及初步临床研究.中华儿科杂志, 2010, 48: 90-94.
[13]Yuan XH, Jin Y, Xie ZP, et al .Prevalence of human KI and WU polymavirus in children with acute respiratory tract infection in China. J Clin Microbiol, 2008, 46: 3522-3525.
[14]Boivin G,De Serres G,Cote S,et al Human metapneumovirus infections in hospitalized children[J].Emerg Infect Dis,2003,9:634-640.
[15]Perirs JS,Tang WH,Chan KH,et al Children with respiratory disease associated with metapneumovirus in Hong Kong[J].Emerg Infect Dis,2003,9:628-633.
[16]Zambon MC,Stockton JD,Clewley JP,et al.Contribution of influenza and respiratory syncytial virus to community cares of influenza like illness:all observational study[J].Lancet,2001;358(7254):1410-1416.
[17]Sonoda S,Get oh Y,Bann F,et al.Acute lower respiratory infections in hospitalized children over a 6 year period in Tokyo[J].Pediatr Int,1999;41(5):519-524.
[1] Shay D K, Holman R C , Newman R D , et al. Bronchiolitis-associated hospitalizations among US children ,1980-1996. JAMA ,1999 , 282: 1440-1446.
[2] Shay D K, Holman R C, Newman R C, et al. Bronchiolitis-associated hospitalizations among American Indian and Alaska Native children. Pediatr Infect Dis J, 2000, 19: 11-17.
[3] Van den Hoogen B G, de Jong J C , Groen J , et al. A newly discovered human pneumovirus isolated from young children with respiratory tract disease. Nat Med , 2001 , 7 : 719-724.
[4] Ksiazek T G,Erdman D,Goldsmith C S,et al.A novel coronavirus associated with severe acute respiratory syndrome.N Engl J Med,2003,348:1953-1966.
[5] Van der Hoek L,Pyrc K,Jebbink M F, et al.Identification of a new human coronanavirus.Nat Med,2004,10:368-373.
[6] Woo P C,Lau S K,Chu C M,et al.Characterization and complete genome sequence of a novel coronavirus,coronavirus HKU1,from patients with pneumonia.J Virol,2005,79:884-895.
[7] Allander T , Tammi M T , Eriksson M , et al. Cloning of a human parvovirus by molecular screening of respiratory tract samples . Proc Natl Acad Sci USA , 2005 , 102 :12891-12896.
[8] Renwick N,Schweiger B,Kapoor V,et al.A recently identified rhinovirus genotype is associated with severe respiratory-tract infection in children in Germany. J Infect Dis,2007 ,196;1754-1760.
[9] Allander T,Andreasson K,Gupta S,et al.Identification of a third human polyomavirus.J Virol,2007,81:4130-4136.
[10] Gaynor A M , Nissen M D , Whiley D M , et al. Identification of a novel polyomavirus from patients with acute respiratory tract infections . PLoS Pathol ,2007 , 3: e64.
[11] Bialasiewicz S, Whiley DM, Lambert SB, et al. Presence of the newly discovered human polyomavirus KI and WU in Australian patients with acute respiratory tractinfection. J Clin Virol, 2008, 41 :63-68.
[12] Han TH, Chung JY, Koo JW, et al. WU Polyomavirus in Children with Acute Lower Respiratory Tract Infections, South Korea. Emerg Infect Dis,2007,13: 1766-1768.
[13] Abed Y, Wang W, Boivin G. WU Polyomavirus in Children, Canada. Emerg Infect Dis, 2007, 13:1939-1941.
[14] Neske F, Blessing K, Ullrich F, et al. WU Polyomavirus Infection in Children, Germany. Emerg Infect Dis,2008,14 :680-681.
[15] Foulongne V, Brieu N, Jeziorski E, et al. KI and WU Polyomaviruses in Children, France. Emerg Infect Dis,2008, 14:523-525.
[16]林峰,郑美云,李桦,等.WU多瘤病毒在中国浙江地区的发现与鉴定.病毒学报,2008 ,24:69-71.
[17] Zhao LQ, Qian Y, Zhu RN,et al. Identification of WU polymavirus from pediatric patients with acute respiratory infections in Beijing,China.Original Article,2010,155:181-186.
[18]贾雪,陆学东,王琼,等.广东地区首例WU多瘤病毒的检出与鉴定.中华检验医学杂志,2009,32:1169-1170.
[19] Gardner SD, Field AM, Coleman DV, Hulme B. New human papovavirus(B.K.) isolated from urine after renal transplantation. Lancet,1971,1:1253–7.
[20] Padgett BL, Walker DL, Zurhein GM, Eckorade RJ, Dessel BH.Cultivation of papova-like virus from human brain with progressivemultifocal leucoencephalopathy. Lancet, 1971,1: 1257–60.
[21]庄婉莉,陆学东,林广裕,等.WU多瘤病毒在儿童急性呼吸道感染的检出及初步临床研究.中华儿科杂志,2010 ,48:90-94.
[22] Le BM, Demertzis LM, Wu G, et al. Clinical and Epidemiologic Characterization of WU Polyomavirus Infection, St. Louis, Missouri. Emerg Infect Dis , 2007 , 13: 1936–1938.
[23] Marieke M.van der Zalm,John W.A.Rossen,BartE.van Ewijk ,et al. Prevalence and Pathogenicity of WU and KI Polyomaviruses in Children,the Netherlands. Emerg Infect Dis,2008,14:1787-1789.
[24] Yuan X H, Jin Y, Xie Z P, et al .Prevalence of Human KI and WU Polyomaviruses inChildren with Acute Respiratory Tract Infection in China. J Clin Microbiol ,2008,46:3522-3525.
[25] Muhanmed Babakir-Mina,Massimo Ciccozzi,Elisabetta Trento, et al .KI and WU Polyomaviruses in Patients Infected with HIV-1,Italy. Emerg Infect Dis,2009 ,15:1323-1325.
[26] Ren LL, Gonzalez R, Xu XW, et al. WU Polyomavirus in Fecal Specimens of Children with Acute Gastroenteritis,China. Emerg Infect Dis, 2009,15:134-135.
[27] Payungporn S, Chieochansin T, Thongmee C, et al. Detection and discrimination of Wu/Ki polyomaviruses by real-time PCR with melting curve analysis.Virological Methods J,2008 ,153:70-73.
[28] Nguyen NL, Le BM, Wang D. Serologic Evidence of Frequent Hu,man Infection with WU and KI Polyomaviruses. Emerg Infect Dis,2009 ,15:1199-1205.