病程相关蛋白质在水稻发育及与白叶枯病菌互作过程中的表达研究
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摘要
背景:水稻是世界上最重要的粮食作物之一,为世界一半以上人口提供主粮。随着水稻测序工作的完成,了解水稻蛋白质的功能将成为生物学研究的重要内容。由Xanthomonas oryzae pv.oryzae (Xoo)引起的水稻白叶枯病,是一种严重的细菌性病害。水稻遭受白叶枯病菌侵染会严重减产,甚至绝收,对抗病基因介导的抗病分子机理研究对水稻生产具有重要的意义。
研究目的:制备了10个针对水稻PR蛋白质的抗体,了解它们的表达模式,筛选参与Xa21介导的水稻抗病途径的PR蛋白质,同时为新的水稻PR蛋白质分类提供实验证据。
材料与方法:通过表达融合蛋白质或合成抗原决定簇,制备了10个PR蛋白质特异抗体,首先调查了相应蛋白质在水稻叶片发育过程中的表达谱,进而调查了它们在水稻Xa21介导的白叶枯病抗性反应不同时间点的表达。
结果:发现大部分PR蛋白质都在叶片生长的不同时期有表达,并且,PR1、PR2、PR3、PR4b、PR8和PR-pha等6个蛋白质在抗性反应后期呈上调表达或有修饰条带的上调表达,比较它们在抗、感和Mock反应中的表达,发现其在抗、感反应中的变化均明显大于Mock反应,且感病反应的变化大多大于抗病反应,没有检测到PR5、PR6、PR15和PR16等4个蛋白质在抗性反应中的表达变化,但在感病反应中,PR5和PR16蛋白质的表达与抗病、Mock不同,在抗、感和Mock反应中没有检测到PR6和PR16蛋白质的表达变化。
结论:病程相关蛋白质(PR)往往在植物遭受病原体侵染后诱导产生。PR1、PR2、PR3、PR4b、PR8和PR-pha等6个蛋白质在抗、感反应中均发挥作用,其表达程度与抗、感反应相关。在感病反应中,PR5和PR16蛋白质的表达与抗病、Mock不同,推测二者在感病反应中发挥作用;在抗、感和Mock反应中没有检测到PR6和PR16蛋白质的表达变化。本实验结果揭示了PR蛋白质的表达与水稻白叶枯病菌互作过程中抗感反应的相关性。
Background: Rice(Oryza sativa)is a very important crop, rice disease can reduce the yield of rice, it can even result in the lost of harvest. With the completion of the Rice Genome Project, the next step forward is to define the biological function of target proteins. Rice bacterial blight is one of the most important rice diseases.The study to the mechanism of rice disease resistance gene has great significances.
Aim: Ten antibodies which are PR proteins-specific were prepared in our endeavour to understand the expression of PR proteins in rice. Then we screened PR protein which associated with the Xa21-mediated resistance against bacterial leaf blight. And we provided experimental evidence for classification of recently found PR protein in rice.
Material and method: we prepared 10 antibodies of PR protein-specific that used fusion protein expression or polypeptide synthesis. We monitored the developmental expression profiles of the corresponding proteins in rice leaves, further we examined their expression at different time points in the Xa21-mediated resistance against bacterial leaf blight.
Results: We found that most of them expressed at different developmental stages of the leaves. Moreover the expression of PR1、PR2、PR3、PR4b、PR8 and PR-pha or their modified bands were up-regulated in the later period of the resistance reaction. Comparing their expression in the resistance, susceptible and Mock responses, we found that changes in resistance and susceptible responses were more obvious than those in Mock response. Furthermore, most of the changes in the susceptible response were more obvious than those in the disease resistance response. Changes of expression in resistance response were not detected in PR5、PR6、PR15 and PR16, during the susceptible response, expression of PR5 and PR16 was different from that of disease resistance and Mock. We didn’t detect the expression changes of PR6 and PR16 proteins in the resistance, susceptible and Mock responses.
Conclusion: Pathogenesis-related proteins (PR_s) are often induced when plants are subjected to pathogen infection. PR1, PR2, PR3, R4b, PR8 and PR-pha proteins played a role in both of the resistance and susceptible responses, which are related with the degrees of their expression. During the susceptible response, expression of PR5 and PR16 was different from that of disease resistance and Mock. So we inferred that both of them played a role in the susceptible response; we didn’t detect the expression changes of PR6 and PR16 proteins in the resistance, susceptible and Mock responses. Our study revealed that the expression of PR proteins is relevant to the resistance and susceptible responses in the interaction between rice and Xanthomonas oryzae pv. Oryzae (Xoo).
研究目的:制备了10个针对水稻PR蛋白质的抗体,了解它们的表达模式,筛选参与Xa21介导的水稻抗病途径的PR蛋白质,同时为新的水稻PR蛋白质分类提供实验证据。
材料与方法:通过表达融合蛋白质或合成抗原决定簇,制备了10个PR蛋白质特异抗体,首先调查了相应蛋白质在水稻叶片发育过程中的表达谱,进而调查了它们在水稻Xa21介导的白叶枯病抗性反应不同时间点的表达。
结果:发现大部分PR蛋白质都在叶片生长的不同时期有表达,并且,PR1、PR2、PR3、PR4b、PR8和PR-pha等6个蛋白质在抗性反应后期呈上调表达或有修饰条带的上调表达,比较它们在抗、感和Mock反应中的表达,发现其在抗、感反应中的变化均明显大于Mock反应,且感病反应的变化大多大于抗病反应,没有检测到PR5、PR6、PR15和PR16等4个蛋白质在抗性反应中的表达变化,但在感病反应中,PR5和PR16蛋白质的表达与抗病、Mock不同,在抗、感和Mock反应中没有检测到PR6和PR16蛋白质的表达变化。
结论:病程相关蛋白质(PR)往往在植物遭受病原体侵染后诱导产生。PR1、PR2、PR3、PR4b、PR8和PR-pha等6个蛋白质在抗、感反应中均发挥作用,其表达程度与抗、感反应相关。在感病反应中,PR5和PR16蛋白质的表达与抗病、Mock不同,推测二者在感病反应中发挥作用;在抗、感和Mock反应中没有检测到PR6和PR16蛋白质的表达变化。本实验结果揭示了PR蛋白质的表达与水稻白叶枯病菌互作过程中抗感反应的相关性。
Background: Rice(Oryza sativa)is a very important crop, rice disease can reduce the yield of rice, it can even result in the lost of harvest. With the completion of the Rice Genome Project, the next step forward is to define the biological function of target proteins. Rice bacterial blight is one of the most important rice diseases.The study to the mechanism of rice disease resistance gene has great significances.
Aim: Ten antibodies which are PR proteins-specific were prepared in our endeavour to understand the expression of PR proteins in rice. Then we screened PR protein which associated with the Xa21-mediated resistance against bacterial leaf blight. And we provided experimental evidence for classification of recently found PR protein in rice.
Material and method: we prepared 10 antibodies of PR protein-specific that used fusion protein expression or polypeptide synthesis. We monitored the developmental expression profiles of the corresponding proteins in rice leaves, further we examined their expression at different time points in the Xa21-mediated resistance against bacterial leaf blight.
Results: We found that most of them expressed at different developmental stages of the leaves. Moreover the expression of PR1、PR2、PR3、PR4b、PR8 and PR-pha or their modified bands were up-regulated in the later period of the resistance reaction. Comparing their expression in the resistance, susceptible and Mock responses, we found that changes in resistance and susceptible responses were more obvious than those in Mock response. Furthermore, most of the changes in the susceptible response were more obvious than those in the disease resistance response. Changes of expression in resistance response were not detected in PR5、PR6、PR15 and PR16, during the susceptible response, expression of PR5 and PR16 was different from that of disease resistance and Mock. We didn’t detect the expression changes of PR6 and PR16 proteins in the resistance, susceptible and Mock responses.
Conclusion: Pathogenesis-related proteins (PR_s) are often induced when plants are subjected to pathogen infection. PR1, PR2, PR3, R4b, PR8 and PR-pha proteins played a role in both of the resistance and susceptible responses, which are related with the degrees of their expression. During the susceptible response, expression of PR5 and PR16 was different from that of disease resistance and Mock. So we inferred that both of them played a role in the susceptible response; we didn’t detect the expression changes of PR6 and PR16 proteins in the resistance, susceptible and Mock responses. Our study revealed that the expression of PR proteins is relevant to the resistance and susceptible responses in the interaction between rice and Xanthomonas oryzae pv. Oryzae (Xoo).
引文
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[2] A E. Pathogenesis-Related Proteins:Research Progress in the Last 15 Years[J]. Gen Appl Plant Physiology, 2005,31:105-134.
[3] C V L L, A V S E. The Families of Pathogenesis-Related Proteins,Their Activities, and Comparative Analysis of Pr-1 Type Proteins[J]. Physiological and Molecular Plant Pathology, 1999,55(2): 13.
[4] Grenier J, Potvin C, Trudel J, et al. Some Thaumatin-Like Proteins Hydrolyse Polymeric Beta-1,3-Glucans[J]. Plant J, 1999,19(4): 473-480.
[5] Bucher G L, Tarina C, Heinlein M, et al. Local Expression of Enzymatically Active Class I Beta-1, 3-Glucanase Enhances Symptoms of Tmv Infection in Tobacco[J]. Plant J, 2001,28(3): 361-369.
[6] Kauffmann S, Legrand M, Geoffroy P, et al. Biological Function of ;Pathogenesis-Related' Proteins: Four Pr Proteins of Tobacco Have 1,3-Beta-Glucanase Activity[J]. EMBO J, 1987,6(11): 3209-3212.
[7] Broekaert W F, Terras F R G,A C B P. Induced and Preformed Antimicrobial Proteins[J]. Mechanisms of resistance to plant diseases, 2000,133:371-478.
[8] Grover A,R G. Strategies for Development of Fungus-Resistant Transgenic Plants[J]. Current Science, 2003,84:330-340.
[9] Trudel J, Grenier J, Potvin C, et al. Several Thaumatin-Like Proteins Bind to Beta-1,3-Glucans[J]. Plant Physiol, 1998,118(4): 1431-1438.
[10] Menu-Bouaouiche L, Vriet C, Peumans W J, et al. A Molecular Basis for the Endo-Beta 1,3-Glucanase Activity of the Thaumatin-Like Proteins from Edible Fruits[J]. Biochimie, 2003,85(1-2): 123-131.
[11] Jorda L, Conejero V,Vera P. Characterization of P69e and P69f, Two Differentially Regulated Genes Encoding New Members of the Subtilisin-Like Proteinase Family from Tomato Plants[J]. Plant Physiol, 2000,122(1): 67-74.
[12] Pasonen H L, Seppanen S K, Degefu Y, et al. Field Performance of Chitinase Transgenic Silver Birches (Betula Pendula): Resistance to Fungal Diseases[J]. Theor Appl Genet, 2004,109(3): 562-570.
[13] Melchers L S,Stuiver M H. Novel Genes for Disease-Resistance Breeding[J]. Curr Opin Plant Biol, 2000,3(2): 147-152.
[14] Passardi F, Penel C,Dunand C. Performing the Paradoxical: How Plant Peroxidases Modify the Cell Wall[J]. Trends Plant Sci, 2004,9(11): 534-540.
[15] Moiseyev G P, Fedoreyeva L I, Zhuravlev Y N, et al. Primary Structures of Two Ribonucleases from Ginseng Calluses. New Members of the Pr-10 Family of Intracellular Pathogenesis-Related Plant Proteins[J]. FEBS Lett, 1997,407(2): 207-210.
[16] Lay F T,Anderson M A. Defensins--Components of the Innate Immune System in Plants[J]. Curr Protein Pept Sci, 2005,6(1): 85-101.
[17] Thomma B P, Cammue B P,Thevissen K. Plant Defensins[J]. Planta, 2002,216(2): 193-202.
[18] Epple P, Apel K,Bohlmann H. Overexpression of an Endogenous Thionin Enhances Resistance of Arabidopsis against Fusarium Oxysporum[J]. Plant Cell, 1997,9(4): 509-520.
[19] Bohlmann H. The Role of Thionins in Plant Protection[J]. Critical reviews in plant sciences, 1994,13:17.
[20] Hoffmann-Sommergruber K. Pathogenesis-Related (Pr) Proteins Identified as Allergens[J]. Biochemical Society Transactions, 2002,30:930-935.
[21] Garcia-Olmedo F, Molina A, Segura A, et al. The Defensive Role of Nonspecific Lipid-Transfer Proteins in Plants[J]. Trends Microbiol, 1995,3(2): 72-74.
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