富集金属锂微生物菌株的筛选及其富集特性的研究
摘要
对采集自新疆阿尔泰山脉可可托海锂矿区周边的土壤样品,进行分离、筛选抗金属锂及富集锂的微生物菌株(包括细菌、放线菌、真菌、酵母)。并对筛选所得细菌菌株进行形态学鉴定、金属锂耐受性、金属锂富集能力和富集培养条件进行了研究。
经分离筛选得到一株可在3000mg·L-1锂浓度下存活的芽孢杆菌,编号为NA1。初步研究表明,该菌对金属锂具有很强的抗性和富集能力。对NA1菌株金属锂耐受性进行研究,结果表明,NA1在液体培养时,培养基初始锂浓度在3.05mg·L-1以下时对其生长无抑制作用,当锂浓度达到106.8mg·L-1时有较大影响,菌体生长基本停止;对冷冻干燥和烘干干燥两种预处理方式对菌体富集锂的影响进行了研究,研究结果显示,此两种预处理方式对菌体富集金属锂的影响非常有限;对NA1摇瓶发酵条件进行了优化,结果表明,在30℃,pH6.0,摇床转速120r/min条件下培养72h,每克干菌体金属锂富集量可达329.6μmol。
本研究为细菌富集金属锂提供了新的材料来源和相关信息,为利用微生物进行盐湖卤水提取(回收)锂奠定了一定基础。
Microoganism (including bacterium, actinomycete, fungi, and yeast) were isolated and screened from soil samples which collected from surrounding area of Keketuohai Lithium-diggings in Altai, Xinjiang. At the same time, the morphology, lithium metal tolerance, lithium-microbial accumulation and enrichment cultivate conditions of the isolated bacterium were investigated.
One strain of bacillus which could survival with 3000mg·L-1 concentration of lithium was designated NA1. Investigation shows that it has high ability of lithium resistance and accumulation. The results of Lithium resistance experiment of NA1 show that it can growth without inhibition in the initial lithium concentration of 3.05mg·L-1 or below, but cell growth stopped when the lithium concentration achieved 106.8 mg·L-1. We also studied the influence of pre-treatment methods of freeze-drying and drying on lithium accumulation of the cell. Results show that the effect was very limited. At the same time, the fermentation conditions of NA1 were also optimized, the results show that under the optimal culture conditions of 30℃, pH6, shaking speed 120r/min, cultivated 72h, the content of lithium-rich reach amount 329.6μmol every gram dry mass.
This study provides new material and related information for lithium concentration of bacteria and lays the foundation for extract (recovery) of lithium by the use of microorganisms from Salt Lake brine.
经分离筛选得到一株可在3000mg·L-1锂浓度下存活的芽孢杆菌,编号为NA1。初步研究表明,该菌对金属锂具有很强的抗性和富集能力。对NA1菌株金属锂耐受性进行研究,结果表明,NA1在液体培养时,培养基初始锂浓度在3.05mg·L-1以下时对其生长无抑制作用,当锂浓度达到106.8mg·L-1时有较大影响,菌体生长基本停止;对冷冻干燥和烘干干燥两种预处理方式对菌体富集锂的影响进行了研究,研究结果显示,此两种预处理方式对菌体富集金属锂的影响非常有限;对NA1摇瓶发酵条件进行了优化,结果表明,在30℃,pH6.0,摇床转速120r/min条件下培养72h,每克干菌体金属锂富集量可达329.6μmol。
本研究为细菌富集金属锂提供了新的材料来源和相关信息,为利用微生物进行盐湖卤水提取(回收)锂奠定了一定基础。
Microoganism (including bacterium, actinomycete, fungi, and yeast) were isolated and screened from soil samples which collected from surrounding area of Keketuohai Lithium-diggings in Altai, Xinjiang. At the same time, the morphology, lithium metal tolerance, lithium-microbial accumulation and enrichment cultivate conditions of the isolated bacterium were investigated.
One strain of bacillus which could survival with 3000mg·L-1 concentration of lithium was designated NA1. Investigation shows that it has high ability of lithium resistance and accumulation. The results of Lithium resistance experiment of NA1 show that it can growth without inhibition in the initial lithium concentration of 3.05mg·L-1 or below, but cell growth stopped when the lithium concentration achieved 106.8 mg·L-1. We also studied the influence of pre-treatment methods of freeze-drying and drying on lithium accumulation of the cell. Results show that the effect was very limited. At the same time, the fermentation conditions of NA1 were also optimized, the results show that under the optimal culture conditions of 30℃, pH6, shaking speed 120r/min, cultivated 72h, the content of lithium-rich reach amount 329.6μmol every gram dry mass.
This study provides new material and related information for lithium concentration of bacteria and lays the foundation for extract (recovery) of lithium by the use of microorganisms from Salt Lake brine.
引文
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