忍冬种质资源调查及遗传多样性的初步研究
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摘要
目的:在忍冬种质资源调查的基础上对忍冬种质资源的遗传多样性和亲缘关系进行初步研究。方法:采用形态分类法对不同忍冬种质的形态特征进行观察描述;用AFLP分子标记对13个忍冬种质进行分析,利用DPS V3.01软件计算各种质的遗传距离,按类平均法(UPGMA)进行聚类分析。结果:忍冬种质间存在明显的形态差异。首次建立了适合忍冬的AFLP银染技术体系。从64对引物组合中筛选得到6对引物的扩增片段具有丰富的多态性,共获得435条扩增带,其中191条具有多态性,平均多态检出率为43.9%,各种质间遗传距离的变化范围在0.0170～0.7143之间。系统树图显示,13个种质可按地域性分为山东与河南新密两大类,同一道地产区栽培种质的亲缘关系较近,山东种质又分为四大组,结果与形态分类结果基本一致。结论:形态差异是划分忍冬种质的重要宏观依据。AFLP分子标记揭示了忍冬种内存在丰富的遗传多样性和种质间的亲缘关系,为忍冬的种质鉴定和品种选育提供了分子水平的理论依据。
Objective: To study the genetic diversity and relationship of the germplasms of Lonicera japonica Thunb. based on the resource investigation. Methods: The morphological characters of germplasms of Lonicera japonica Thunb. were surveyed and described by the means of morphological taxonomy.Amplified fragment length polymorphism (AFLP) was carried out to analyze 13 germplasms of Lonicera japonica Thunb..DPS V3.01 software was used to estimate the genetic distance. Unweighed pair group method with arithmetic mean (UPGMA) cluster analysis was used to construct a dendrogram. Results: There are obvious morphological differences among germplasms of Lonicera japonica Thunb.. The AFLP silver-staining technique system fitting for genetic DNA analysis of Lonicera japonica Thunb. was established for the first time. Six selected primer combinations showing high level of polymorphism were selected out form 64 primer combinations. A total of 435 bands were obtained including 191 polymorphic loci. The average polymorphic frequency was 43.9%. The genetic distances ranged from 0.0170～0.7143 among the germplasms. Dendrogram revealed that 13 germplasms could be classified regionally as“Shandong”and“Henan xinmi”two main groups. The relationship of the cultivated variety from the same genuine area was close. The germplasms from Shandong could be classified 4 groups and the classification result based on AFLP marker was basically consistent with those on their morphological characters. Conclusion: The morphological differences are the important macroscopical evidence for distinguishing germplasms of Lonicera japonica Thunb..AFLP marker can indicate the abundant genetic diversity in the species and relationship among the germplasms of Lonicera japonica Thunb..The results provide theoretical evidence for identification the germplasms and for breeding new cultivar of Lonicera japonica Thunb. on molecular level.
Objective: To study the genetic diversity and relationship of the germplasms of Lonicera japonica Thunb. based on the resource investigation. Methods: The morphological characters of germplasms of Lonicera japonica Thunb. were surveyed and described by the means of morphological taxonomy.Amplified fragment length polymorphism (AFLP) was carried out to analyze 13 germplasms of Lonicera japonica Thunb..DPS V3.01 software was used to estimate the genetic distance. Unweighed pair group method with arithmetic mean (UPGMA) cluster analysis was used to construct a dendrogram. Results: There are obvious morphological differences among germplasms of Lonicera japonica Thunb.. The AFLP silver-staining technique system fitting for genetic DNA analysis of Lonicera japonica Thunb. was established for the first time. Six selected primer combinations showing high level of polymorphism were selected out form 64 primer combinations. A total of 435 bands were obtained including 191 polymorphic loci. The average polymorphic frequency was 43.9%. The genetic distances ranged from 0.0170～0.7143 among the germplasms. Dendrogram revealed that 13 germplasms could be classified regionally as“Shandong”and“Henan xinmi”two main groups. The relationship of the cultivated variety from the same genuine area was close. The germplasms from Shandong could be classified 4 groups and the classification result based on AFLP marker was basically consistent with those on their morphological characters. Conclusion: The morphological differences are the important macroscopical evidence for distinguishing germplasms of Lonicera japonica Thunb..AFLP marker can indicate the abundant genetic diversity in the species and relationship among the germplasms of Lonicera japonica Thunb..The results provide theoretical evidence for identification the germplasms and for breeding new cultivar of Lonicera japonica Thunb. on molecular level.
引文
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