不同孔隙度多孔钛种植体对骨整合影响的研究
摘要
目的:研究两种不同孔隙度多孔钛种植体经酸碱热处理仿生沉积羟基磷灰石对骨整合的影响。
材料和方法:采用粉末注射成形技术制备孔隙度分别为40%(A组)和60%(B组)的多孔钛种植体,采用酸碱热处理、仿生沉积羟基磷灰石(hydorxyapatite,RA)的方法进行表面改性,将种植体植入狗背部肌肉及股骨内,分别于4、8、12周后处死动物,取出标本,进行大体标本X线片、软组织切片HE染色、双色荧光标记荧光显微镜、不脱钙硬组织切片Goldner's三色法染色检测及定量组织学骨长入深度测定。
结果:软组织切片HE染色检测显示:4、8、12周背部肌肉内种植体无明显炎症反应和组织坏死。双色荧光标记检测显示:在植入4周时,近种植体的宿主骨表面有荧光素沉积,而种植体表面荧光素沉积不明显。植入8周时,两组种植体的表面和多孔结构表层的空隙内见荧光物质沉积,植入12周时,A组仅在种植体表层孔隙内可见荧光,而B组在种植体深层孔隙内亦有荧光素沉积。不脱钙硬组织切片检测显示:4周时,A、B两组种植体与宿主骨间均存在间隙,间隙内可见纤维结缔组织;8周时,A、B两组种植体与宿主骨间间隙减小,在种植体表面及近表面的孔隙内,可见未矿化的类骨质形成,而深层孔隙内多为纤维结缔组织;12周时,A、B两组种植体均与骨组织实现骨整合,A组种植体的新生骨组织仅长入表层的孔隙,深层孔隙内仅见少量类骨质及纤维组织,而B组种植体深层孔隙内可见钙化良好的新生骨组织,其间可见血管穿行。定量组织学测定结果表明,4周时,两组种植体骨长入深度均为0;8周时,A组与B组骨长入深度比较差异无显著性(P＞0.05);12周时,两组骨长入深度比较差异有显著性(P＜0.05),B组宿主骨与种植体间骨长入深度大于A组。
结论:经表面改性的两种不同孔隙度的多孔钛种植体植入骨质内12周时均能实现骨整合,新生骨组织能长入多孔结构,形成生物固定;60%孔隙度的多孔钛种植体更有利于新生骨组织长入多孔结构。
Objective:This study investigated the effect of different porosity on the osseointegration of porous titanium implant after surface modification.
Material and method:This study utilized the Metal Injection Molding technology to prepare porous tiantium implants with the porosity of 40%and 60%;carried out the surface modification by means of acid-base heat treatment,biomimetic deposition hydroxyapatite;implanted them in the back muscle and femur of dogs.The animals were sacrificed at 4,8,12 weeks after implantation.Bone formation in the implants was investigated by x-ray test, histology and histomorphometry of non-decalcified sections using traditional light- and fluorescence microscopy.
Results:Soft tissue sections using HE staining showed:all of the back muscle implants had no inflammation and necrosis at 4,8 and 12 weeks.That two-color fluorescent markers detection showed:at 4 weeks after the implantation,there was fluorescence deposition on the surface of the host bone near the implants,but the fluorescence deposition on the implants was not obvious.At 8 weeks after the implantation,it showed that there was fluorescence deposition both on the surface and external pores of porous implant.At 12 weeks,for group A,there was only fluorescence deposition in the external pores of implant;for group B,the fluorescence can also be found in the deep-seated pores.Non-decalcified sections detected:at 4 week,there was a gap between implants and the host bone in two groups,the fibrous connective tissue could be seen in the gap.For 8 weeks after the implantation, the gap between the implant and the host bone got narrow.It can be seen that the osteoid formed on the surface and external pores of the implant.But the pores of deeper layers were filled with fibrous connective tissue.At 12 weeks after implantation,both of group A and B had completed the osseointegration. The new-grown bone tissue of group A only grew into the external pores and only a little osteoid existed in the deep-seated pores.For group B,the visible well-calcified bone tissue existed in the pores both surface and deep layers,the micrangium could be seen inside of it.The Quantitative histological determination showed:after 4 weeks,for two groups,the depth of the bone ingrowth in the implants was 0.At 8 weeks,the depth of bone ingrowth didn't have been distincted diversity between group A and B(p＞0.05).At 12 weeks, the bone ingrowth depth showed a remarkable difference between group A and B(p＜0.05).The depth of the bone ingrowth of group B was deeper than group A.
Conclusion:The surface-modified porous titanium implant with the different porosity can complete osseointegration within 12 weeks.The new-born bone tissue can grow into the porous structure,and achieve biological fixation.The porous titanium implant with 60%porosity connective pore structure is benefit to new bone tissue grow into the porous structure.
材料和方法:采用粉末注射成形技术制备孔隙度分别为40%(A组)和60%(B组)的多孔钛种植体,采用酸碱热处理、仿生沉积羟基磷灰石(hydorxyapatite,RA)的方法进行表面改性,将种植体植入狗背部肌肉及股骨内,分别于4、8、12周后处死动物,取出标本,进行大体标本X线片、软组织切片HE染色、双色荧光标记荧光显微镜、不脱钙硬组织切片Goldner's三色法染色检测及定量组织学骨长入深度测定。
结果:软组织切片HE染色检测显示:4、8、12周背部肌肉内种植体无明显炎症反应和组织坏死。双色荧光标记检测显示:在植入4周时,近种植体的宿主骨表面有荧光素沉积,而种植体表面荧光素沉积不明显。植入8周时,两组种植体的表面和多孔结构表层的空隙内见荧光物质沉积,植入12周时,A组仅在种植体表层孔隙内可见荧光,而B组在种植体深层孔隙内亦有荧光素沉积。不脱钙硬组织切片检测显示:4周时,A、B两组种植体与宿主骨间均存在间隙,间隙内可见纤维结缔组织;8周时,A、B两组种植体与宿主骨间间隙减小,在种植体表面及近表面的孔隙内,可见未矿化的类骨质形成,而深层孔隙内多为纤维结缔组织;12周时,A、B两组种植体均与骨组织实现骨整合,A组种植体的新生骨组织仅长入表层的孔隙,深层孔隙内仅见少量类骨质及纤维组织,而B组种植体深层孔隙内可见钙化良好的新生骨组织,其间可见血管穿行。定量组织学测定结果表明,4周时,两组种植体骨长入深度均为0;8周时,A组与B组骨长入深度比较差异无显著性(P＞0.05);12周时,两组骨长入深度比较差异有显著性(P＜0.05),B组宿主骨与种植体间骨长入深度大于A组。
结论:经表面改性的两种不同孔隙度的多孔钛种植体植入骨质内12周时均能实现骨整合,新生骨组织能长入多孔结构,形成生物固定;60%孔隙度的多孔钛种植体更有利于新生骨组织长入多孔结构。
Objective:This study investigated the effect of different porosity on the osseointegration of porous titanium implant after surface modification.
Material and method:This study utilized the Metal Injection Molding technology to prepare porous tiantium implants with the porosity of 40%and 60%;carried out the surface modification by means of acid-base heat treatment,biomimetic deposition hydroxyapatite;implanted them in the back muscle and femur of dogs.The animals were sacrificed at 4,8,12 weeks after implantation.Bone formation in the implants was investigated by x-ray test, histology and histomorphometry of non-decalcified sections using traditional light- and fluorescence microscopy.
Results:Soft tissue sections using HE staining showed:all of the back muscle implants had no inflammation and necrosis at 4,8 and 12 weeks.That two-color fluorescent markers detection showed:at 4 weeks after the implantation,there was fluorescence deposition on the surface of the host bone near the implants,but the fluorescence deposition on the implants was not obvious.At 8 weeks after the implantation,it showed that there was fluorescence deposition both on the surface and external pores of porous implant.At 12 weeks,for group A,there was only fluorescence deposition in the external pores of implant;for group B,the fluorescence can also be found in the deep-seated pores.Non-decalcified sections detected:at 4 week,there was a gap between implants and the host bone in two groups,the fibrous connective tissue could be seen in the gap.For 8 weeks after the implantation, the gap between the implant and the host bone got narrow.It can be seen that the osteoid formed on the surface and external pores of the implant.But the pores of deeper layers were filled with fibrous connective tissue.At 12 weeks after implantation,both of group A and B had completed the osseointegration. The new-grown bone tissue of group A only grew into the external pores and only a little osteoid existed in the deep-seated pores.For group B,the visible well-calcified bone tissue existed in the pores both surface and deep layers,the micrangium could be seen inside of it.The Quantitative histological determination showed:after 4 weeks,for two groups,the depth of the bone ingrowth in the implants was 0.At 8 weeks,the depth of bone ingrowth didn't have been distincted diversity between group A and B(p＞0.05).At 12 weeks, the bone ingrowth depth showed a remarkable difference between group A and B(p＜0.05).The depth of the bone ingrowth of group B was deeper than group A.
Conclusion:The surface-modified porous titanium implant with the different porosity can complete osseointegration within 12 weeks.The new-born bone tissue can grow into the porous structure,and achieve biological fixation.The porous titanium implant with 60%porosity connective pore structure is benefit to new bone tissue grow into the porous structure.
引文
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