运动改善视黄醇结合蛋白4（RBP4）诱导的胰岛素抵抗的机制研究
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摘要
目的：探讨耐力运动处方以及耐力运动+抗阻运动处方对超重/肥胖青年血清视黄醇结合蛋白4的影响,以及运动处方干预前后视黄醇结合蛋白4与体重、体脂比、BMI、胰岛素抵抗指数、血脂指标、腰围、腰臀比、最大摄氧量等指标之间的关系,明确视黄醇结合蛋白4与胰岛素抵抗之间的关系,以及不同运动处方干预后的变化规律,为运动处方通过影响视黄醇结合蛋白4改善胰岛素抵抗提供理论依据。探讨耐力运动处方对视黄醇结合蛋白4诱导的胰岛素抵抗大鼠骨骼肌、脂肪和肝脏葡萄糖摄取以及胰岛素受体、胰岛素受体底物1蛋白表达和磷酸化的影响；探讨耐力运动处方对视黄醇结合蛋白4诱导的胰岛素抵抗大鼠骨骼肌磷酸肌醇-3-激酶、蛋白酪氨酸磷酸酶表达的影响；探讨耐力运动处方对视黄醇结合蛋白4诱导的胰岛素抵抗大鼠肝脏葡萄糖激酶和磷酸烯醇式丙酮酸羧激酶表达和肝糖原的影响,揭示运动改善视黄醇结合蛋白4诱导的胰岛素抵抗机制。方法：采用BMI为诊断标准,筛选符合标准的超重/肥胖大学生93名；正常体重大学生31名。有氧运动训练组进行12周健身操训练,有氧运动+抗阻肌肉力量训练组进行12周健身操+抗阻肌肉力量训练,运动前后检测实验对象各项指标。重组视黄醇结合蛋白4活体注射的方法获得胰岛素抵抗大鼠模型,随机分为运动组和安静对照组,另设正常安静对照组大鼠。运动组进行3周游泳运动干预,检测大鼠血清视黄醇结合蛋白4、胰岛素抵抗指数等生化指标；免疫组化法分析大鼠骨骼肌、脂肪和肝脏胰岛素受体、胰岛素受体底物1蛋白表达和磷酸化情况,骨骼肌磷酸肌醇-3-激酶、蛋白酪氨酸磷酸酶蛋白表达情况,检测骨骼肌、脂肪和肝脏葡萄糖摄取情况；免疫组化法分析大鼠肝脏葡萄糖激酶和磷酸烯醇式丙酮酸羧激酶表达情况,检测肝糖原含量。结果：(1)运动干预前超重/肥胖者血清视黄醇结合蛋白4水平与体重、BMI、体脂比、胰岛素抵抗指数、腰围、空腹胰岛素、空腹血糖、胆固醇、低密度脂蛋白胆固醇呈正相关,与最大摄氧量、高密度脂蛋白胆固醇呈负相关。(2)12周有氧运动训练显著减少超重/肥胖者血清视黄醇结合蛋白4、体重、BMI、体脂比、空腹胰岛素、胆固醇、甘油三酯、低密度脂蛋白胆固醇、胰岛素抵抗指数、腰围、腰臀比；显著增加最大摄氧量、高密度脂蛋白胆固醇。12周有氧运动+抗阻肌肉力量训练显著减少超重/肥胖者血清视黄醇结合蛋白4、体重、BMI、体脂比、空腹胰岛素、空腹血糖、胆固醇、甘油三酯、低密度脂蛋白胆固醇、胰岛素抵抗指数、腰围、腰臀比；显著增加最大摄氧量、高密度脂蛋白胆固醇。(3)2种运动处方干预后,腰围、腰臀比、空腹血糖、胆固醇、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇均值差异具有显著性。其它指标均值差异无显著性。(4)有氧运动干预后,超重/肥胖者血清视黄醇结合蛋白4与体重、BMI、腰围、空腹胰岛素、胰岛素抵抗指数呈正相关,与最大摄氧量呈负相关。有氧运动+抗阻肌肉力量训练干预后,超重/肥胖者血清视黄醇结合蛋白4与体重、BMI、腰围、空腹胰岛素、胰岛素抵抗指数呈正相关,与最大摄氧量呈负相关。下降的血清视黄醇结合蛋白4与下降的体重、BMI、体脂比、胰岛素、胰岛素抵抗指数呈正相关。(5)运动组和安静组大鼠血清视黄醇结合蛋白4、胰岛素抵抗指数显著高于正常对照组；运动组大鼠血清视黄醇结合蛋白4、胰岛素抵抗指数显著低于安静组；运动组和安静组大鼠骨骼肌细胞和肝细胞基础状态和胰岛素刺激状态的葡萄糖摄取率均显著低于正常对照组组；与安静组相比,运动组骨骼肌细胞和肝细胞基础状态和胰岛素刺激状态的葡萄糖摄取率显著增加。(6)运动组和安静组大鼠骨骼肌和肝脏胰岛素受体、受体底物1蛋白表达和磷酸化比正常对照组显著减少,运动组大鼠骨骼肌和肝脏胰岛素受体蛋白表达和磷酸化与安静组相比无显著差异。运动组大鼠骨骼肌胰岛素受体底物1磷酸化蛋白表达与安静组相比显著增加。(7)运动组和安静组大鼠骨骼肌磷酸肌醇-3-激酶蛋白表达、葡萄糖摄取率比正常对照组显著减少,运动组大鼠骨骼肌磷酸肌醇-3-激酶蛋白表达、葡萄糖摄取率与安静组相比显著增加。(8)运动组和安静组大鼠骨骼肌蛋白酪氨酸磷酸酶1B蛋白表达比正常对照组显著增加,葡萄糖摄取率显著减少；运动组大鼠骨骼肌蛋白酪氨酸磷酸酶1B蛋白表达与安静组相比显著减少,葡萄糖摄取率显著增加。(9)运动组和安静组大鼠肝脏葡萄糖激酶蛋白表达和活性比正常对照组显著减少,运动组大鼠肝脏葡萄糖激酶蛋白表达和活性与安静组相比显著增加。运动组和安静组大鼠肝脏磷酸烯醇式丙酮酸羧激酶蛋白表达和活性比正常对照组显著增加,运动组大鼠肝脏葡萄糖激酶蛋白表达和活性与安静组相比显著减少。运动组和安静组大鼠肝糖原含量比正常对照组显著减少,运动组大鼠肝糖原含量显著高于安静组。结论：(1)有氧运动和有氧运动+抗阻肌肉力量训练2种运动处方均能够显著降低超重／肥胖青年血清视黄醇结合蛋白4、体重、BMI、体脂比、空腹胰岛素、空腹血糖、胆固醇、甘油三酯、胰岛素抵抗指数、腰围、腰臀比；增加最大摄氧量和高密度脂蛋白胆固醇,改善胰岛素抵抗；2种运动处方干预后,2组超重／肥胖青年间血清视黄醇结合蛋白4水平未出现显著差异。(2)有氧运动和有氧运动+抗阻肌肉力量训练2种运动处方干预后,下降的血清视黄醇结合蛋白4与下降的体重、BMI、体脂比、胰岛素、胰岛素抵抗指数呈正相关。(3)视黄醇结合蛋白4显著抑制大鼠骨骼肌细胞和肝细胞胰岛素受体、胰岛素受体底物1和磷酸肌醇-3-激酶的蛋白表达以及胰岛素受体底物1的磷酸化,降低骨骼肌细胞和肝细胞的葡萄糖摄取率,导致胰岛素抵抗。3周游泳运动干预能够通过解除视黄醇结合蛋白4对大鼠骨骼肌细胞胰岛素受体底物1磷酸化和磷酸肌醇-3-激酶的蛋白表达的抑制,增加骨骼肌细胞葡萄糖摄取率,改善胰岛素抵抗。(4)视黄醇结合蛋白4显著增加大鼠骨骼肌细胞蛋白酪氨酸磷酸酶1B的蛋白表达,降低骨骼肌细胞葡萄糖摄取率,导致胰岛素抵抗。3周游泳运动干预能够降低被视黄醇结合蛋白4增加的大鼠骨骼肌细胞蛋白酪氨酸磷酸酶1B的蛋白表达,增加骨骼肌细胞葡萄糖摄取率,改善胰岛素抵抗。(5)视黄醇结合蛋白4显著抑制大鼠肝脏葡萄糖激酶的表达和活性,增加肝脏磷酸烯醇式丙酮酸羧激酶的表达和活性,减少肝糖合成,增加肝糖异生。3周游泳运动干预能够增加被视黄醇结合蛋白4抑制的大鼠肝脏葡萄糖激酶的表达和活性,降低被视黄醇结合蛋白4增加的大鼠肝脏磷酸烯醇式丙酮酸羧激酶的表达和活性,增加肝糖合成,减少肝糖异生。
Objective To study the effects of aerobic exercise and aerobic exercise+resistance exercise on serum RBP4 in overweight/obesity young man.To discuss the relationship between RBP4 and body mass, body fat percent,blood lipid,body mass index,insulin resistance index,waist circumferenc,waist-hip ratio and V02max before and after exercise prescription.To research the change law and than to provide theory foundation that exercise presciption could improve insulin resistance through the influence in RBP4.To study the effects of aerobic exercise on glucose intake,expression and phosphorylation of insulin receptor and insulin receptor substrate1 in skeletal muscle,adipose tissue and liver of RBP4-induced insulin resistance rats.To study the effects of aerobic exercise on expression of phosphoric acid inositol-3-kinase and protein tyrosine phosphatase 1B in RBP4-induced insulin resistance rats skeletal muscle.To study the effects of aerobic exercise on hepatic glycogen, expression of glucokinase and phosphoenolpyruvate carboxykinase in RBP4-induced insulin resistance rats liver.To proclaim the mechanism that exercise improve RBP4-induced insulin resistance.Method According to BMI,ninety-three overweight/obesity university students were enroled as standard objects.Objects consist of forty-five fale and forty-eight female.Fifteen fale and sixteen female normal weight university students were enroled.Twelve weeks aerobics proceeded in Aerobic exercise group.Twelve weeks aerobics+resisitance exercise proceeded in aerobic exercise+resistance exercise group.Serum RBP4,body mass,body fat percent,body mass index,insulin resistance index,blood lipide,waist circumference,waist-hip ratio and VO2max detected before and after exercise. Recombination RBP4 injected rats to gain insulin resistance rats model. Insulin resistance rats divided into exercise group and quiet group,normal rats enroled as control group.Serum RBP4,insulin resistance index were detected after three weeks swimming.Expression and phosphorylation of insulin receptor and insulin receptor substance1 in skeletal muscle,adipose tissue and liver.Expression of phosphoric acid inositol-3-kinase, protein tyrosine phosphatase 1B and glucose intake were detected in skeletal muscle.Expression of glucokinase, phosphoenolpyruvate carboxykinase and hepatic glycogen were detected in liver.Results (1) There was positive relationshop between serum RBP4 and body mass,body mass index,body fat percent,insulin resistance index,waistcircumference,insulin,blood glucose,cholesterol,low density lipoprotein cholesterol after exercise intervene.There was negative relationship between serum RBP4 and high density lipoprotein cholesterol. (2)Twelve weeks aerobic exercise could notably decrease serum RBP4,body mass,body mass index,body fat percent,insulin, cholesterol,triglyceride,low density lipoprotein cholesterol,insulin resistance index,waist circumference,waist-hip ratio,and could notably increase V02max and high density lipoprotein cholesterol in overweight/obesity yonug university students.Twelve weeks aerobic exercise+resistance exercise could notably decrease serum RBP4,body mass,body massindex,body fat percent,insulin,blood glucose,cholesterol,triglyceride,low density lipoprotein cholesterol, insulin resistance index,waist circumference,waist-hip ratio,and could notably increase V02max and high density lipoprotein cholesterol in overweight/obesity yonug university students. (3) There was not significant difference in RPB4,body mass,body mass index,body fat percent,insulin, insulin resistance index,cholestrol,triglyceride,VO2max between aerobic exercise group and aerobic+resistance exercise group.There was significant difference in waist circumference,waist-hip ratio,blood gucose,high density lipoprotein cholestrol and low density lipoprotein choletrol between aerobic exercise group and aerobic+resistance exercise group. (4) There was positive relationship between RBP4 and body mass,body mass index,waist circumference,insulin, insulin resistance index after aerobic exercise in overweight/obesity. There was negtive relationship between RBP4 and V02max after aerobic exercise in overweight/obesity.There was positive relationship between RBP4 and body mass,body mass index,waist circumference,insulin, insulin resistance index after aerobic+resistance exercise in overweight/obesity. There was negtive relationship between RBP4 and V02max after aerobic+resistance exercise in overweight /obesity.There was positive relationship between decreasing RBP4 and decreasing body mass after aerobic+resistance exercise in overweight/obesity. There was positive relationship betweenΔRBP4 andΔbody mass,ΔBM,Δbody fat percentd,Δinsulin andΔinnsulin resistance index after aerobic+resistance exercise intervene. (5) Serum RBP4 and insulin resistance index increased more in exercise group rats and quiet group rats than control group rats.Serum RBP4 and insulin resistance index decreased more in exercise group rats than quiet group rats.Serum RBP4 and HOMA-IR increasd more in RBP4 intraperitoneal injection exercise groups than nomal control group, respetively.Glucose intake ratio in muscle cells and hepatic cells stimulating with or without insulin decresed more in exercise group rats and quiet group rats than control group.Glucose intake ratio in muscle cells and hepatic cells stimulating with or without insulin increase more in exercise group rats than quiet group rats. (6) Expression and phosphorylation of skeletal cells insulin receptor and insulin receptor substrate 1 decreased more in exercise group rats and quiet group rats than control group rats.There was not difference in expression and phosphorylation of skeletal cells insulin receptor between exercise group rats and quiet group rats.Phosphorylation of skeletal muscle insulin receptor 1 increased more in exercise group rats than quiet rats. (7) Expression of Phosphoric acid inositol-3-kinase and glucose intake ratio decreased more in exercise group rats and quiet group rats' skeletal cells than control group rats.Expression of Phosphoric acid inositol-3-kinase and glucose intake ratio increased more in exercise group rats' skeletal cells than quiet group rats. (8) Expression of protein tyrosine phosphatase IB increased more in exercise group rats and quiet group rats' skeletal cells than control group rats.Glucose intake ratio decreased more in exercise group rats and quiet group rats' skeletal cells than control group rats.Expression of protein tyrosine phosphatase 1B decreased more in exercise group rats' skeletal cells than quiet group rats.Glucose intake ratio increased more in exercise group rats' skeletal cells than quiet group rats. (9) Expression and activity of glucokinase decreased more in exercise group rats and quiet group rats' liver than control group rats.Expression and activity of glucokinase increased more in exercise group rats' liver than quiet group rats.Expression and activity of phosphoenolpyruvate carboxykinase increased more in exercise group rats and quiet group rats' liver than control group rats.Expression and activity of phosphoenolpyruvate carboxykinase decreased more in exercise group rats' liver than quiet group rats.Hepatic glycogen decreased more in exercise group rats and quiet group rats' liver than control group rats.Hepatic glycogen increased more in exercise group rats' liver than quiet group rats.Conclusion (1) Aerobic exercise and aerobic+resistance exercise could notably improve insulin resistance by decreasing serum RBP4,body mass, body mass index,bodyfat percent,insulin,bloodglucose,cholesterol,triglyceride, innsulin resistance index,waist circumference,waist-hip ratio of overweight/obesity youth.Aerobic exercise and aerobic+resistance exercise could notably improve insulin resistance by increasing V02max and high density lipoprotein cholesterol of overweight/obesity youth.There was not difference in serum RBP4 between aerobic exercise group and aerobic+resistance exercise group after exercise intervene. (2) There was positive relationship betweenΔRBP4 andΔbody mass,ΔBM,Δbody fat percentd,Δinsulin andΔinnsulin resistance index after aerobic+resistance exercise intervene. (3) RBP4 could result in insulin resistance by notable suppression the expression and phosphorylation of insulin receptor,insulin receptor substrate 1,Phosphoric acid inositol-3-kinase and glucose intake ratio in skeletal cells and hepatic cells.Three weeks swimming could improve insulin resistance through removing the suppression in insulin receptor substrate 1 phosphorylation and Phosphoric acid inositol-3-kinase expression of skeletal cell by RBP4 and through increasing skeletal cell glucose intake ratio. (4) RBP4 could result in insulin resistance by notable increase the expression of protein tyrosine phosphatase 1B and decrease glucose intake ratio in skeletal cell.Three weeks swimming could improve insulin resistance through removing the increase in protein tyrosine phosphatase 1B expression of skeletal cell by RBP4 and through increasing skeletal cell glucose intake. (5) RBP4 could notably restrain expression and activity of glucokinase in liver,increase expression and activity of phosphoenolpyruvate carboxykinase,decrease glycogen synthetize and increase glycogen heteroplasia.Three weeks swimming could increase expression and activity suppressed of glucokinase by RBP4,decrease expression and activity of phosphoenolpyruvate carboxykinase increased by RBP4,increase glycogen synthetize and decrease glycogen heteroplasia.
Objective To study the effects of aerobic exercise and aerobic exercise+resistance exercise on serum RBP4 in overweight/obesity young man.To discuss the relationship between RBP4 and body mass, body fat percent,blood lipid,body mass index,insulin resistance index,waist circumferenc,waist-hip ratio and V02max before and after exercise prescription.To research the change law and than to provide theory foundation that exercise presciption could improve insulin resistance through the influence in RBP4.To study the effects of aerobic exercise on glucose intake,expression and phosphorylation of insulin receptor and insulin receptor substrate1 in skeletal muscle,adipose tissue and liver of RBP4-induced insulin resistance rats.To study the effects of aerobic exercise on expression of phosphoric acid inositol-3-kinase and protein tyrosine phosphatase 1B in RBP4-induced insulin resistance rats skeletal muscle.To study the effects of aerobic exercise on hepatic glycogen, expression of glucokinase and phosphoenolpyruvate carboxykinase in RBP4-induced insulin resistance rats liver.To proclaim the mechanism that exercise improve RBP4-induced insulin resistance.Method According to BMI,ninety-three overweight/obesity university students were enroled as standard objects.Objects consist of forty-five fale and forty-eight female.Fifteen fale and sixteen female normal weight university students were enroled.Twelve weeks aerobics proceeded in Aerobic exercise group.Twelve weeks aerobics+resisitance exercise proceeded in aerobic exercise+resistance exercise group.Serum RBP4,body mass,body fat percent,body mass index,insulin resistance index,blood lipide,waist circumference,waist-hip ratio and VO2max detected before and after exercise. Recombination RBP4 injected rats to gain insulin resistance rats model. Insulin resistance rats divided into exercise group and quiet group,normal rats enroled as control group.Serum RBP4,insulin resistance index were detected after three weeks swimming.Expression and phosphorylation of insulin receptor and insulin receptor substance1 in skeletal muscle,adipose tissue and liver.Expression of phosphoric acid inositol-3-kinase, protein tyrosine phosphatase 1B and glucose intake were detected in skeletal muscle.Expression of glucokinase, phosphoenolpyruvate carboxykinase and hepatic glycogen were detected in liver.Results (1) There was positive relationshop between serum RBP4 and body mass,body mass index,body fat percent,insulin resistance index,waistcircumference,insulin,blood glucose,cholesterol,low density lipoprotein cholesterol after exercise intervene.There was negative relationship between serum RBP4 and high density lipoprotein cholesterol. (2)Twelve weeks aerobic exercise could notably decrease serum RBP4,body mass,body mass index,body fat percent,insulin, cholesterol,triglyceride,low density lipoprotein cholesterol,insulin resistance index,waist circumference,waist-hip ratio,and could notably increase V02max and high density lipoprotein cholesterol in overweight/obesity yonug university students.Twelve weeks aerobic exercise+resistance exercise could notably decrease serum RBP4,body mass,body massindex,body fat percent,insulin,blood glucose,cholesterol,triglyceride,low density lipoprotein cholesterol, insulin resistance index,waist circumference,waist-hip ratio,and could notably increase V02max and high density lipoprotein cholesterol in overweight/obesity yonug university students. (3) There was not significant difference in RPB4,body mass,body mass index,body fat percent,insulin, insulin resistance index,cholestrol,triglyceride,VO2max between aerobic exercise group and aerobic+resistance exercise group.There was significant difference in waist circumference,waist-hip ratio,blood gucose,high density lipoprotein cholestrol and low density lipoprotein choletrol between aerobic exercise group and aerobic+resistance exercise group. (4) There was positive relationship between RBP4 and body mass,body mass index,waist circumference,insulin, insulin resistance index after aerobic exercise in overweight/obesity. There was negtive relationship between RBP4 and V02max after aerobic exercise in overweight/obesity.There was positive relationship between RBP4 and body mass,body mass index,waist circumference,insulin, insulin resistance index after aerobic+resistance exercise in overweight/obesity. There was negtive relationship between RBP4 and V02max after aerobic+resistance exercise in overweight /obesity.There was positive relationship between decreasing RBP4 and decreasing body mass after aerobic+resistance exercise in overweight/obesity. There was positive relationship betweenΔRBP4 andΔbody mass,ΔBM,Δbody fat percentd,Δinsulin andΔinnsulin resistance index after aerobic+resistance exercise intervene. (5) Serum RBP4 and insulin resistance index increased more in exercise group rats and quiet group rats than control group rats.Serum RBP4 and insulin resistance index decreased more in exercise group rats than quiet group rats.Serum RBP4 and HOMA-IR increasd more in RBP4 intraperitoneal injection exercise groups than nomal control group, respetively.Glucose intake ratio in muscle cells and hepatic cells stimulating with or without insulin decresed more in exercise group rats and quiet group rats than control group.Glucose intake ratio in muscle cells and hepatic cells stimulating with or without insulin increase more in exercise group rats than quiet group rats. (6) Expression and phosphorylation of skeletal cells insulin receptor and insulin receptor substrate 1 decreased more in exercise group rats and quiet group rats than control group rats.There was not difference in expression and phosphorylation of skeletal cells insulin receptor between exercise group rats and quiet group rats.Phosphorylation of skeletal muscle insulin receptor 1 increased more in exercise group rats than quiet rats. (7) Expression of Phosphoric acid inositol-3-kinase and glucose intake ratio decreased more in exercise group rats and quiet group rats' skeletal cells than control group rats.Expression of Phosphoric acid inositol-3-kinase and glucose intake ratio increased more in exercise group rats' skeletal cells than quiet group rats. (8) Expression of protein tyrosine phosphatase IB increased more in exercise group rats and quiet group rats' skeletal cells than control group rats.Glucose intake ratio decreased more in exercise group rats and quiet group rats' skeletal cells than control group rats.Expression of protein tyrosine phosphatase 1B decreased more in exercise group rats' skeletal cells than quiet group rats.Glucose intake ratio increased more in exercise group rats' skeletal cells than quiet group rats. (9) Expression and activity of glucokinase decreased more in exercise group rats and quiet group rats' liver than control group rats.Expression and activity of glucokinase increased more in exercise group rats' liver than quiet group rats.Expression and activity of phosphoenolpyruvate carboxykinase increased more in exercise group rats and quiet group rats' liver than control group rats.Expression and activity of phosphoenolpyruvate carboxykinase decreased more in exercise group rats' liver than quiet group rats.Hepatic glycogen decreased more in exercise group rats and quiet group rats' liver than control group rats.Hepatic glycogen increased more in exercise group rats' liver than quiet group rats.Conclusion (1) Aerobic exercise and aerobic+resistance exercise could notably improve insulin resistance by decreasing serum RBP4,body mass, body mass index,bodyfat percent,insulin,bloodglucose,cholesterol,triglyceride, innsulin resistance index,waist circumference,waist-hip ratio of overweight/obesity youth.Aerobic exercise and aerobic+resistance exercise could notably improve insulin resistance by increasing V02max and high density lipoprotein cholesterol of overweight/obesity youth.There was not difference in serum RBP4 between aerobic exercise group and aerobic+resistance exercise group after exercise intervene. (2) There was positive relationship betweenΔRBP4 andΔbody mass,ΔBM,Δbody fat percentd,Δinsulin andΔinnsulin resistance index after aerobic+resistance exercise intervene. (3) RBP4 could result in insulin resistance by notable suppression the expression and phosphorylation of insulin receptor,insulin receptor substrate 1,Phosphoric acid inositol-3-kinase and glucose intake ratio in skeletal cells and hepatic cells.Three weeks swimming could improve insulin resistance through removing the suppression in insulin receptor substrate 1 phosphorylation and Phosphoric acid inositol-3-kinase expression of skeletal cell by RBP4 and through increasing skeletal cell glucose intake ratio. (4) RBP4 could result in insulin resistance by notable increase the expression of protein tyrosine phosphatase 1B and decrease glucose intake ratio in skeletal cell.Three weeks swimming could improve insulin resistance through removing the increase in protein tyrosine phosphatase 1B expression of skeletal cell by RBP4 and through increasing skeletal cell glucose intake. (5) RBP4 could notably restrain expression and activity of glucokinase in liver,increase expression and activity of phosphoenolpyruvate carboxykinase,decrease glycogen synthetize and increase glycogen heteroplasia.Three weeks swimming could increase expression and activity suppressed of glucokinase by RBP4,decrease expression and activity of phosphoenolpyruvate carboxykinase increased by RBP4,increase glycogen synthetize and decrease glycogen heteroplasia.
引文
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