两种药用植物的化学成分及其生物活性研究
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摘要
本论文对两种药用植物:蒲公英属植物蒙古蒲公英(Taraxacummongolicum Hand.-Mazz.)和姜属植物生姜(Zingiber officinale Rosc.)进行了研究。
蒲公英(Herba Taraxaci)为我国常用中药材,种类繁多,资源丰富。在国内以蒙古蒲公英(简称蒲公英)为主流品。国外对药用蒲公英(Taraxacumofficinale)的化学成分研究比较全面,但药理活性报道较少,而未见蒙古蒲公英的化学成分研究报道;国内对蒲公英属植物的化学成分研究报道甚少,多引用Taraxacum officinale的化学成分。为了进一步阐明蒲公英的药效物质基础,本研究对蒙古蒲公英进行了系统的植物化学研究和体外细胞毒活性研究。从中分离得到28个单体化合物,鉴定了其中的26个化合物的结构,其中化合物(Ⅰ-12～Ⅰ-13)为首次从本属植物中分得,化合物(Ⅰ-17～Ⅰ-18)为首次从该种植物中分得。蒲公英的次生代谢产物主要是黄酮类和酚酸类化合物。体外细胞毒活性实验结果表明:化合物Ⅰ-2和化合物Ⅰ-10对肿瘤细胞株有较强的抑制作用。
生姜是姜科姜属多年生草本植物,在我国资源十分丰富,全国各地都有栽培,而在民间仅广泛用作调味品,因此极具开发利用价值,我们研究了云南省罗平县该植物的化学成分及其抗氧化活性,从中分离并鉴定了9个单体化合物。抗氧化活性研究结果表明:化合物Ⅱ-3有较强的抗氧化活性。
This thesis studied two medicinal plants,Taraxacum mongolicum Hand.-Mazz.and Zingiber officinale Rosc.
Herba Taraxaci,which has a long history as a Traditional Chinese Medicine, has different species as well as rich resources in China.Among which,the most popular species is Taraxacum mongolicum.Previous phytochemical examinations on this genus were concentrated on Taraxacum officinale.In order to find out the active components of T.mongolicum,its phytochemical purification was performed and 26 compounds were isolated and structurally elucidated. CompoundsⅠ-12 andⅠ-13 were isolated from Taraxacum species for the first time. CompoundsⅠ-17 andⅠ-18 were isolated from T.mongolicum for the first time.It can be seen from the above results,the flavonoids and phenolic acids are the main metabolisms of Taraxacum mongolicum.The in vitro cytotoxicity investigation on these constituents from mongolicum was also carried out.CompoundsⅠ-2 and I-10 showed strong inhibitory action to tumor cells.
Zingiber officinale is a plant belonging to Zingiber genus of Zingiberaceae. In Traditional Chinese Medicine(TCM),it is considered as a pungent,dry, warming,yang herb to be used for ailments triggered by cold,damp weather.To make a better use of Zingiber officinale,we studied on the chemical constituents and antioxidant activities of the material bought in luoping county,Yunnan province.9 compounds were isolated and structurally elucidated.CompoundⅡ-3 showed strong antioxidant activity.
蒲公英(Herba Taraxaci)为我国常用中药材,种类繁多,资源丰富。在国内以蒙古蒲公英(简称蒲公英)为主流品。国外对药用蒲公英(Taraxacumofficinale)的化学成分研究比较全面,但药理活性报道较少,而未见蒙古蒲公英的化学成分研究报道;国内对蒲公英属植物的化学成分研究报道甚少,多引用Taraxacum officinale的化学成分。为了进一步阐明蒲公英的药效物质基础,本研究对蒙古蒲公英进行了系统的植物化学研究和体外细胞毒活性研究。从中分离得到28个单体化合物,鉴定了其中的26个化合物的结构,其中化合物(Ⅰ-12～Ⅰ-13)为首次从本属植物中分得,化合物(Ⅰ-17～Ⅰ-18)为首次从该种植物中分得。蒲公英的次生代谢产物主要是黄酮类和酚酸类化合物。体外细胞毒活性实验结果表明:化合物Ⅰ-2和化合物Ⅰ-10对肿瘤细胞株有较强的抑制作用。
生姜是姜科姜属多年生草本植物,在我国资源十分丰富,全国各地都有栽培,而在民间仅广泛用作调味品,因此极具开发利用价值,我们研究了云南省罗平县该植物的化学成分及其抗氧化活性,从中分离并鉴定了9个单体化合物。抗氧化活性研究结果表明:化合物Ⅱ-3有较强的抗氧化活性。
This thesis studied two medicinal plants,Taraxacum mongolicum Hand.-Mazz.and Zingiber officinale Rosc.
Herba Taraxaci,which has a long history as a Traditional Chinese Medicine, has different species as well as rich resources in China.Among which,the most popular species is Taraxacum mongolicum.Previous phytochemical examinations on this genus were concentrated on Taraxacum officinale.In order to find out the active components of T.mongolicum,its phytochemical purification was performed and 26 compounds were isolated and structurally elucidated. CompoundsⅠ-12 andⅠ-13 were isolated from Taraxacum species for the first time. CompoundsⅠ-17 andⅠ-18 were isolated from T.mongolicum for the first time.It can be seen from the above results,the flavonoids and phenolic acids are the main metabolisms of Taraxacum mongolicum.The in vitro cytotoxicity investigation on these constituents from mongolicum was also carried out.CompoundsⅠ-2 and I-10 showed strong inhibitory action to tumor cells.
Zingiber officinale is a plant belonging to Zingiber genus of Zingiberaceae. In Traditional Chinese Medicine(TCM),it is considered as a pungent,dry, warming,yang herb to be used for ailments triggered by cold,damp weather.To make a better use of Zingiber officinale,we studied on the chemical constituents and antioxidant activities of the material bought in luoping county,Yunnan province.9 compounds were isolated and structurally elucidated.CompoundⅡ-3 showed strong antioxidant activity.
引文
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