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水貂胰蛋白酶消化模型的建立及外源酶对水貂消化和生产性能的影响
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摘要
本文通过四个试验对水貂消化道参数与生产性能的研究,来建立水貂胰蛋白酶消化模型及确定外源酶对水貂消化和生产性能的影响,分为以下几个主要部分:
     试验一:水貂胰蛋白酶作用模型的建立。试验采用66只水貂,采食后每隔30分钟测定300分钟内消化道内蛋白酶活性与pH值,反映水貂采食后消化道内消化规律的变化。由试验结果可知,水貂采食后消化道前段pH值为酸性,随采食时间的延长,pH值呈现先升高后降低的变化,但一直保持酸性pH值;消化道后段pH值为碱性,采食后其pH值随时间变化规律与消化道前段则正相反,呈现先变低,后升高的趋势,但一直保持碱性pH值;pH值转变时间大约在采食后90~120分钟;水貂采食后消化道各部位蛋白酶活性逐渐升高,大约在采食后60~120分钟时间段内达到最高,以后逐渐降低,在降低的过程中存在活性升高的波峰出现;水貂消化道各部位蛋白酶活性以回肠部位最高。
     试验二:外源酶对水貂生产性能及肠道主要消化参数的影响。试验采用3×3复因子试验设计,在水貂传统日粮中添加外源酶,通过生产性能指标、消化道各部位的胰蛋白酶活性与pH值来验证添加外源酶对水貂生产性能与对水貂消化的影响。试验结果表明,添加外源酶对水貂的总体生产性能未产生明显影响,但对公貂活体长产生了极显著的影响,中水平蛋白酶添加组比对照组公貂活体长提高了9.15%;对水貂母貂胃蛋白酶活性并无显著影响;对公貂间胃蛋白活性的影响达到极显著水平,添加脂肪酶组公貂胃蛋白活性最高;对水貂十二指肠胰蛋白活性、回肠胰蛋白活性、公貂空肠胰蛋白活性并无显著影响;对母貂间空肠胰蛋白活性影响达显著水平,添加低水平淀粉酶与蛋白酶组显著高于其他组;对水貂结肠胰蛋白活性影响达极显著水平,添加蛋白酶与脂肪酶的最高水平组显著高于添加淀粉酶的中低水平组;对水貂公貂直肠胰蛋白活性并无显著影响;对水貂母貂直肠胰蛋白活性影响达极显著水平;对水貂胃内pH值、母貂肠内各部位pH值并无显著影响;对水貂公貂肠内各部位pH值的影响均达极显著水平,添加脂肪酶组与对照组在肠道内以回肠为分界线,回肠以前较高,以后较低;添加淀粉酶组正相反。添加蛋白酶组则在小肠各部位pH值一直保持较低水平。
     试验三:外源酶对水貂糖代谢的影响。本试验采用56只16周龄健康短毛黑水貂,采用2×3复因子试验设计,研究添加外源淀粉酶与蛋白酶对水貂糖代谢水平的影响。结果表明,在育成期与冬毛生长期水貂传统日粮类型条件下,添加试验用淀粉酶与蛋白酶,对各试验组水貂肝糖元含量、公貂血糖水平影响不显著;对各试验组水貂母貂血糖水平影响显著,(P<0.05)。添加高水平淀粉酶组效果最明显,其血糖水平高于对照组46.83%。
     试验四:外源酶的添加与水貂日粮类型之间的关系。试验采用3×3复因子试验设计,在水貂特定日粮中添加外源酶,通过生产性能指标、营养物质消化率来验证适宜的试验日粮类型及在该日粮类型条件下适宜添加的外源酶类型。结果表明,在水貂冬毛生长期,在本试验日粮类型条件下,添加试验用酶对水貂活体长无显著影响;对各组水貂体重影响达极显著水平。植物性蛋白替代水平越高,水貂越冬体重损失越大;对饲料蛋白质消化率的影响达极显著水平,表现为植物性蛋白质替代水平越高,水貂对饲料中蛋白质的消化率越低,中性蛋白酶可极显著的提高水貂对饲料中蛋白质的消化率。其中添加中性蛋白酶的30%植物性蛋白替代试验组的饲料蛋白质消化率比不添加酶60%植物性蛋白替代试验组提高44.03%;对水貂饲料脂肪消化率的影响达极显著水平,其中添加中性蛋白酶的30%植物性蛋白替代试验组的饲料脂肪消化率比不添加酶60%植物性蛋白替代试验组提高129.45%。
Four experiments were conducted to investigate functional model of trypsinase and effect of extraneous enzyme to digestibility and production performance in mink by determining production performance and primary digestive parameter in alimentary canal of mink.
     Exp.1 Foundation of functional model of trypsinase in mink. 66 minks were allocated rankomly into 11 groups. Activity of trypsinase and pH in enteron was determinated every 30 minutes during 300 minutes after feeding. The data could reflect digestive rule in enteron after feeding. The result of the trail indicts that pH in forepart of enteron is acidic after feeding. pH of forepart of enteron presents increasing first then falling when time is changing after feeding . pH of forepart of enteron keep acidic though PH is changing after feeding. PH of rearward of enteron is alkalescent. pH of rearward of enteron presents falling first then increasing when time is changing after feeding . pH of rearward of enteron keep alkalescent though pH is changing after feeding. pH of enteron changes at 90~120 minutes after feeding . Activity of trypsinase in enteron increases gradually after feeding. It abtains the highest at about 60~120 minutes after feeding. Then it falls gradually. Activity of trypsinase in enteron increase again in the course of falling. Activity of trypsinase in ileum is highest than other parts of enteron.
     Exp.2 Effect of extraneous enzyme to production performance and primary digestive parameter in enteron of mink. The trail adopted 3×3 mulriple factor experimental design. Additive enzyme was added to traditional diet of mink. Production performance and activity of trypsinase and pH in enteron was determined in the trail. Effect of additive enzyme to production performance and digestion of mink was investigated. The result indicts that effect of additive enzyme to total production performance and pepsin activity of female mink is not very significant (P>0.05) . But effect of additive enzyme to length of live male mink is very significant (P<0.01) . Length of live male mink in moderate level papain group is higher 9.15% than antitheses group. Effect of additive enzyme to pepsin activity of male mink is very significant (P<0.01) . Pepsin activity of male mink in additive lipase is highest. Effect of additive enzyme to activity of trypsinase of duodenum and ileum of mink is not very significant (P>0.05) . Effect of additive enzyme to activity of trypsinase of jejunum of male mink is not very significant (P>0.05) . Effect of additive enzyme to activity of trypsinase of jejunum of female mink is significant (P<0.05) . Effect of additive enzyme to activity of trypsinase of colon of mink is very significant (P<0.01) . Activity of trypsinase of colon in low level papain and amylase groups is higher significant than other groups. Effect of additive enzyme to activity of trypsinase of rectum of male mink is not very significant (P>0.05). Effect of additive enzyme to activity of trypsinase of rectum of female mink is very significant (P<0.01) .Effect of additive enzyme to pH in stomach is not very significant (P>0.05) . Effect of additive enzyme to enteric pH of female mink is not very significant (P>0.05) . Effect of additive enzyme to enteric pH of male mink is very significant (P<0.01) .Enteron is divided into two parts regarding ileum as borderline. pH in forepart of enteron in additive lipase group and antitheses group is higher than other groups. pH in rearward enteron in additive lipase group and antitheses group is lower than other groups. pH of enteron in additive amylase group is antipodal with pH of enteron in additive lipase group and antitheses group. pH of enteron in additive papain group always keeps low level.
     Exp.3 Effect of extraneous enzyme to sugar metabolism in mink. 56 16-week-old minks were allocated rankomly into 7 groups. The trail adopted 2×3 mulriple factor experimental design. Effect of additive amylase and papain to sugar metabolism in mink was investigated. The result indicts that effect of additive amylase and papain to glycogenic content of mink is not significant (P>0.05) when feed is traditional diet in young mink period. Effect of additive amylase and papain to content of blood sugar of male mink is not significant (P>0.05). Effect of additive amylase and papain to content of blood sugar of female mink is significant (P<0.05). Effect of high level additive amylase to content of blood sugar of mink is more significant. Content of blood sugar of mink in high level additive amylase group is higher 46. 83% than antitheses group.
     Exp.4 Research of relationship between extraneous enzyme and diet type of mink. The trail adopted 3×3 mulriple factor experimental design. Additive enzyme was added to given diet of mink. Production performance and rate of digestive nutritional component was determined in the trail. It was investigated that appropriate diet type and appropriate enzyme type and level when diet type is given in young mink period. The result indicts that effect of additive enzyme to length of live mink is not very significant ( P>0.05 ) when diet type is given in young mink period. Effect of additive enzyme to weight gain of live mink is very significant (P<0.01) . Level of vegetal protein substituteing creatural protein is higher, weight loss is bigger when mink live through the winter. Effect of additive enzyme to rate of digestive protein is very significant(P<0.01). Level of vegetal protein substituteing creatural protein is higher, rate of digestive protein in feed is lower. Rate of digestive protein in neutral papain group is higher significant than other groups. Rate of digestive protein in additive neutral papain and 30% of vegetal protein substituteing creatural protein group is higher 44.03% than no additive enzyme and 60% of vegetal protein substituteing creatural protein group. Effect of additive enzyme to rate of digestive fat is very significant (P<0.01). Rate of digestive fat in additive neutral papain and 30% of vegetal protein substituteing creatural protein group is higher 129.45% than no additive enzyme and 60% of vegetal protein substituteing creatural protein group.
引文
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