水稻细胞质雄性不育系育性稳定性和柱头外露率遗传基础研究
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摘要
本研究以中国香稻和高柱头外露率的川香29B的重组自交系为材料,构建一张遗传连锁图谱。以水稻重组自交系的每个家系为父本分别与母本川香29A杂交,得到细胞质雄性不育为遗传背景的测交F1群体,定位控制自然结实率、套袋结实率、柱头外露率、柱头单露率、柱头双露率、对细胞质雄性不育性育性影响的数量性状位点(QTL)和开花习性,并选育以川香29为主要遗传背景的优良三系不育系。这些结果如下：
1.基于重组自交系,构建了一张包含144个SSR分子标记的遗传连锁图谱,其共覆盖水稻全基因组1884.7 cM,标记间的平均图距为12.9 cM。
2.考察测交F1群体的自然结实率、套袋结实率、花粉可育率,共定位到7个QTL,控制柱头自然结实率的2个QTL分别位于第6,11染色体；控制套袋结实率的2个QTL分别位于第6,11染色体；控制花粉可育率的3个QTL分别位于第3,4,6染色体。基于以上三种表型,同时定位到2个影响不育系育性稳定性的QTL,位于第6染色体的RM585-RM510区间和第11染色体的RM2459-RM26115区间。这2个影响育性稳定性的QTL,其增效基因均来源于中国香稻。
3.考察测交F1群体的柱头外露率、柱头单露率、柱头双露率,共定位到10个QTL。控制柱头外露率的3个QTL分别位于第1,2,11染色体；控制柱头单露率的4个QTL分别位于1,2,11,12号染色体；控制柱头双露率的3个QTL分别位于1,2,11号染色体上。这些QTL解释了6.25到34.85%的全部表型变异。在第1,第2,第11染色体检测到三个区段,能够控制柱头外露率、柱头单露率和柱头双露率,说明这些区段是控制柱头外露性状的主效区域,并且这些区段的增效基因均来源于川香29B。
In this study, based on recombinant inbred lines derived from the cross of Zhongguo Xiangdao and Chuanxiang 29B,a genetic linkage map were constructed. Crossing Chuanxiang 29A with each line of recombinant inbred lines(RILs) in order to make a testcross F1 population withg genetic background of cytoplasmic male sterility to map quantitative trait loci (QTLs) for natural seed-setting rate, bagged seed-setting rate, single stigma exsertion rate, double stigma exsertion rate, stigma exsertion rate and pollen fertility rate and breed sterile lines with genetic background of Chuanxiang 29. These results are as follows:
1.Based on recombinant inbred lines, a genetic linkage map were developed containing 144 SSR markers, which covers a total of 1884.7cM with an average interval of 12.9cM.
2.Investigated natural seed-setting rate, bagged seed-setting rate and pollen fertility rate in the testcross F1 population,7 QTLs were detected. Two QTLs for natural seed-setting rate were located on the chromosome 6,11,respectively; There were 2 QTLs for bagged seed-setting rate, locating on the chromosome 6,11,respectively; three QTLs for pollen fertility rate were identified on chromosome 3,4,6.Based on natural seed-setting rate, baged-setting rate and pollen fertility rate, we identified 2 QTLs related to fertility stability of rice sterile line.Zhongguo Xiangdao alleles of all QTLs for fertility stability of rice sterile line contributed to an increase in each trait and most QTLs showed a small effect.
3.There were 3 QTLs for rice stigma exsertion rate, located on chromosome 1,2, 11;4 QTLs for rice single stigma exsertion rate, located on chromosome 1,2,11,12;3 QTLs of double stigma exsertion rate were located on chromosome 1,2,11.These QTLs expained 6.25 to 34.85% of total phenotypic variation. Three intervals for the three traits were detected on the chromosomel,2,11, indicating these regions were major regions for stigma exsertion. All the favorable QTLs related to rice stigma exsertion were orignated from alleles of Chuanxiang 29B.
1.基于重组自交系,构建了一张包含144个SSR分子标记的遗传连锁图谱,其共覆盖水稻全基因组1884.7 cM,标记间的平均图距为12.9 cM。
2.考察测交F1群体的自然结实率、套袋结实率、花粉可育率,共定位到7个QTL,控制柱头自然结实率的2个QTL分别位于第6,11染色体；控制套袋结实率的2个QTL分别位于第6,11染色体；控制花粉可育率的3个QTL分别位于第3,4,6染色体。基于以上三种表型,同时定位到2个影响不育系育性稳定性的QTL,位于第6染色体的RM585-RM510区间和第11染色体的RM2459-RM26115区间。这2个影响育性稳定性的QTL,其增效基因均来源于中国香稻。
3.考察测交F1群体的柱头外露率、柱头单露率、柱头双露率,共定位到10个QTL。控制柱头外露率的3个QTL分别位于第1,2,11染色体；控制柱头单露率的4个QTL分别位于1,2,11,12号染色体；控制柱头双露率的3个QTL分别位于1,2,11号染色体上。这些QTL解释了6.25到34.85%的全部表型变异。在第1,第2,第11染色体检测到三个区段,能够控制柱头外露率、柱头单露率和柱头双露率,说明这些区段是控制柱头外露性状的主效区域,并且这些区段的增效基因均来源于川香29B。
In this study, based on recombinant inbred lines derived from the cross of Zhongguo Xiangdao and Chuanxiang 29B,a genetic linkage map were constructed. Crossing Chuanxiang 29A with each line of recombinant inbred lines(RILs) in order to make a testcross F1 population withg genetic background of cytoplasmic male sterility to map quantitative trait loci (QTLs) for natural seed-setting rate, bagged seed-setting rate, single stigma exsertion rate, double stigma exsertion rate, stigma exsertion rate and pollen fertility rate and breed sterile lines with genetic background of Chuanxiang 29. These results are as follows:
1.Based on recombinant inbred lines, a genetic linkage map were developed containing 144 SSR markers, which covers a total of 1884.7cM with an average interval of 12.9cM.
2.Investigated natural seed-setting rate, bagged seed-setting rate and pollen fertility rate in the testcross F1 population,7 QTLs were detected. Two QTLs for natural seed-setting rate were located on the chromosome 6,11,respectively; There were 2 QTLs for bagged seed-setting rate, locating on the chromosome 6,11,respectively; three QTLs for pollen fertility rate were identified on chromosome 3,4,6.Based on natural seed-setting rate, baged-setting rate and pollen fertility rate, we identified 2 QTLs related to fertility stability of rice sterile line.Zhongguo Xiangdao alleles of all QTLs for fertility stability of rice sterile line contributed to an increase in each trait and most QTLs showed a small effect.
3.There were 3 QTLs for rice stigma exsertion rate, located on chromosome 1,2, 11;4 QTLs for rice single stigma exsertion rate, located on chromosome 1,2,11,12;3 QTLs of double stigma exsertion rate were located on chromosome 1,2,11.These QTLs expained 6.25 to 34.85% of total phenotypic variation. Three intervals for the three traits were detected on the chromosomel,2,11, indicating these regions were major regions for stigma exsertion. All the favorable QTLs related to rice stigma exsertion were orignated from alleles of Chuanxiang 29B.
引文
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4.李晨,孙传清,穆平,陈亮,王象坤.栽培稻与普通野生稻两个重要分类性状花药长度和柱头外露率的QTL分析.遗传学报,2001,28(8)：746-751.
5.李维明,蔡俊迈.稻雄性不育系天然异交率与花器形状遗传相关研究Ⅰ,福建农学院学报,1988,17(8)：85-92.
6.李陶,陈一吾.水稻柱头外露的遗传研究.作物学报,1987,13(4)：314-321.
7.罗琼,周开达.水稻无内稃突变体的遗传分析和基因定位.遗传学报.2002,29(3)：230-234.
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