红豆杉中与异戊二烯代谢途径相关的AP2类转录调控因子的克隆与功能研究
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摘要
异戊二烯代谢途径(Isoprenoid Biosynthetic Pathway)是植物萜类次生代谢物合成的重要途径,该途径中生成的异戊二烯类代谢产物包括青蒿素、紫杉醇、银杏内酯和其他萜类吲哚生物碱等都具有重要的经济价值。其中,红豆杉(Taxuscuspidata)异戊二烯代谢途径产物之一的紫杉醇(Taxol~(?))因其独特的抗癌机理和广谱性,在临床上被广泛应用,但受红豆杉生长缓慢、紫杉醇含量低和合成成本高等因素的制约,其产量一直难于提高,使得运用基因工程手段来提高紫杉醇生物合成的研究成为近年来次生代谢工程中的研究热点之一。
转录因子(Transcription Factor,TF)作为后基因组中改造植物代谢途径的一种新型工具,具有从宏观调控次生代谢通量的优势。目前与次生代谢途径相关的转录因子研究多集中在苯丙氨酸代谢途径和生物碱代谢途径上,已报道的与萜类合成转录水平调控相关的转录因子只有TIA合成途径中的ORCA1-3家族和ZCT1-3家族,而对于红豆杉中异戊二烯代谢途径相关转录因子的研究还处于空白。本文首次利用酵母单杂交(Yeast One-Hybrid)方法和cDNA末端快速扩增技术(Rapid-Amplification of cDNA Ends,RACE)从东北红豆杉cDNA文库中克隆到两个AP2类转录因子基因,TcAP2和TcDREB,并对它们与红豆杉异戊二烯代谢途径紫杉醇合成途径中的多个关键酶基因的启动子的结合情况进行了研究,为深入研究AP2类转录因子能否参与对紫杉醇合成的转录调控奠定了基础。
以JERE作诱饵,利用酵母单杂交的建库和筛选方法首次从东北红豆杉中克隆到与MeJA诱导相关的AP2类转录因子基因TcAP2,其开放阅读框(OpenReading Frame,ORF)编码长度为268个氨基酸的蛋白,分子量29.6kD,等电点5.05,拓扑结构显示该转录因子具有典型的AP2结构域特征:3个反向平行的β-折叠和1个α-螺旋。其DNA结合域内有DREB亚族特有的Val~(14)和Ala~(37)氨基酸残基,分别是DREB亚族转录因子与DRE元件和GCC Box元件的结合位点。进化树分析及同源比对分析都表明,该蛋白与拟南芥(Arabidopsis thaliana)中的At1g21910基因所编码的AtAP2和小立碗藓(Physcomitrella patens)中的PpAP2转录因子进化关系最近,属于同一分支,其DNA结合域的一致性达到85%以上。通过Southern blot分析表明该基因在东北红豆杉中以单拷贝形式存在。
通过RACE方法从东北红豆杉中克隆到第二个AP2类转录因子基因TcDREB,其ORF编码410个氨基酸的蛋白,也具有典型的AP2结构域,空间构象及结合的顺式元件类型与TcAP2类似。进化树分析和多重比对表明,就DNA结合域而言,该转录因子与长春花(Catharanthus roseus)的ORCA1和大豆(Glycinemax)的GmDREBa的一致性最高。Southern blot表明该基因在东北红豆杉中也以单拷贝形式存在。
亚细胞定位结果表明两个转录因子都定位在细胞核中。半定量RT-PCR分析显示,它们在幼茎中的表达量最高。RT-QPCR(Real-time Quantitative PCR)表明TcAP2转录水平的表达主要受MeJA和SA、高盐和低温的诱导;而TcDREB的表达受高盐、MeJA和SA以及ABA的诱导。凝胶电泳迁移率实验(Electrophoretic Mobility Shift Assays,EMSA)显示,两个转录因子都能与DRE顺式元件结合。其中TcDREB还能与紫杉醇合成途径中的TS、5α、10β和13α四个关键酶基因的启动子以及TS基因启动子上的GCC Box结合,而突变后的GCCBox不结合TcDREB。进一步用原子力显微镜(Amotic Force Microscope,AFM)证实其结合的特异性,发现加入游离的TcDREB蛋白能使其与DRE元件的结合概率从(19.3±5.2)%下降到(3.9±1.3),而与MDRE的结合概率仅为(4.5±1.6)%,结合成键分布概率验证了TcDREB与DRE元件及四个启动子结合的特异性。综合分析认为,TcDREB与红豆杉异戊二烯代谢产物紫杉醇的合成途径相关,并且这种相关性可能是受ABA信号传导途径调控的。对于TcDREB结合启动子上的顺式元件的具体类型还有待进一步启动子缺失的结合实验证明。
为了探明这两个转录因子在红豆杉中与异戊二烯代谢途径的产物紫杉醇的合成途径的相关性,进一步构建了TcAP2基因和TcDREB基因的植物表达载体并转化拟南芥;同时构建了TS、5α、10β和13α四个关键酶基因的启动子融合gus基因的植物表达载体与转录因子基因植物表达载体共转化拟南芥,已获得转TcDREB基因的阳性植株,为进一步研究其在紫杉醇代谢中的作用打下了基础。
Isoprenoid biosynthetic pathway plays an important role in plant terpenoid natural metabolism pathways and terpenoids' synthesis.Most of the products (Artemisinin,Taxol,Ginkgolide) in isoprenoid biosynthetic pathway have been broadly applied in medical field and clinical therapy for human beings.Therefore, studying the isoprenoid biosynthetic pathway in Taxus cuspidata is important for further understanding the regulation and biosynthesis of taxol,the important anti-cancer chemical produced in Taxus trees.
Taxus is an ancient and precious species.Taxol(paclitaxel) is one of natural diterpenoid alkaloids firstly isolated from the bark of the yew(Taxus brevifolia). Taxol has been well established and approved by FDA as a very important effective chemotherapeutic agent against a wide range of tumors since 1992.However,the supply of Taxol has been limited since the discovery of this natural product,and with increasing applications in chemotherapy,the availability and cost of the drug will remain an important issue.
Transcription factors(TFs),found to be regulators of metabolic flux,have become a powerful tool for the manipulation of complex metabolic engineering in plants.In this thesis,two novel AP2 TF genes,TcAP2 and TcDREB,which may be involved in taxol biosynthetic pathway,has been isolated from Taxus cuspidata for the first time.
Probing into the induction effects of methyl jasmonate(MeJA) and salicylic acid (SA) to taxol content,suspension cells of T.cuspidate were treated with exogenous MeJA and SA before construction of yeast one-hybrid library.Based on spatial distribution frequency of C-13 phenylpropanoid side chain-CoA acyltransferase (BAPT),we explored the optimal induction time of MeJA and SA to the suspension cells for the construction of cDNA library.
The bait plasmid was constructed with cis-element JERE and 72 positive plasmids were selected from T.cuspidata cDNA library by using yeast one-hybrid system.A cDNA-coded TF encoding 268 amino acids was obtained.It had a conserved AP2/EREBP domain in which the N-terminal had a 4-amino acid nuclear localization signal(NLS) and C-terminal had a transcriptional activation domain, named as TcAP2(GenBank accession number EU549860).Multiple alignments and phylogenetic tree analysis indicated that TcAP2 was a member of AP2/EREBP transcription factor gene family and the deduced TcAP2 protein had the highest homology with At1g21910 from Arabidopsis thaliana and PpAP2 from Physcomitrella patens,which possibly reflected the similar functions among them. The binding site V14 inβ-sheet of YRG element and A37 in or-helix of RAYD element was conserved in AP2/EREBP domain which reflected its binding ability to DRE cis-element and GCC Box.
Via SMART Rapid Amplification of 5' and 3'-cDNA Ends(RACE),another novel AP2 transcription factor gene,TcDREB(GenBank accession number EU549861),was cloned from T.cuspidata.The full-length cDNA of TcDREB contained a 1233-bp ORF,encoding 410 amino acids with no N-terminal propeptide. Predicted tertiary structure showed it had the conserved AP2 domain like TcAP2. Multiple alignments showed TcDREB was also a member of the DREB subfamily. TcDREB also possessed threeβ-sheets and oneα-helix as well as V14 and A37 DNA binding sites.
Subcellular location analysis of TcAP2 and TcDREB demonstrated that they were nuclear-localized proteins.Semi-quantitative RT-PCR revealed that TcAP2 and TcDREB constitutively expressed in all test tissues,including stems,roots,and leaves, with high expression in new stems.
EMSA of TcAP2 and TcDREB analysis indicated that both of them could bind to DRE element.Besides,TcDREB could bind to the promoters of 10β,13α,5αand TS as well as GCC Box of TS promoter in taxol biosynthesis pathway but not to MGCC Box.Determination of binding specificity was testified by AFM force measurement. The mean value of single molecular interaction force of TcDREB-DRE was determined as(76.3±2.3) pN.The binding probability was(19.3±5.2).The probabilities of TcDREB and other four promoters were ranged from 15%to 20%, suggesting the regulation of TcDREB in taxol biosynthesis pathway of T.cuspidata.
The expression of TcAP2 and TcDREB could be induced by MeJA,low temperature and salt,ABA and salt,suggesting their involvement in isoprenoid biosynthetic pathway and ABA-dependent signal transduction.
In order to analyze the regulation of the two transcription factors in isoprenoid biosynthetic pathway,the over-expression vector containing TcAP2 or TcDREB cDNAs driven by the CaMV 35S promoters and vectors carrying TS/5α/10β/13α-promoter-GUSA were constructed and single or co-transformed into A. thaliana.Transgenic plants transformed with TcDREB over-expression vector were generated,which will be used to test the effects of stress on electrolyte leakage rate in the transgenic plants in further study.
In summary,the cloning and analysis of TcDREB and promoters of 10β,13α,5αand TS provide the basis for further studying the detailed role of TcDREB in regulation of taxol biosynthetic pathway of T.cuspidata.
转录因子(Transcription Factor,TF)作为后基因组中改造植物代谢途径的一种新型工具,具有从宏观调控次生代谢通量的优势。目前与次生代谢途径相关的转录因子研究多集中在苯丙氨酸代谢途径和生物碱代谢途径上,已报道的与萜类合成转录水平调控相关的转录因子只有TIA合成途径中的ORCA1-3家族和ZCT1-3家族,而对于红豆杉中异戊二烯代谢途径相关转录因子的研究还处于空白。本文首次利用酵母单杂交(Yeast One-Hybrid)方法和cDNA末端快速扩增技术(Rapid-Amplification of cDNA Ends,RACE)从东北红豆杉cDNA文库中克隆到两个AP2类转录因子基因,TcAP2和TcDREB,并对它们与红豆杉异戊二烯代谢途径紫杉醇合成途径中的多个关键酶基因的启动子的结合情况进行了研究,为深入研究AP2类转录因子能否参与对紫杉醇合成的转录调控奠定了基础。
以JERE作诱饵,利用酵母单杂交的建库和筛选方法首次从东北红豆杉中克隆到与MeJA诱导相关的AP2类转录因子基因TcAP2,其开放阅读框(OpenReading Frame,ORF)编码长度为268个氨基酸的蛋白,分子量29.6kD,等电点5.05,拓扑结构显示该转录因子具有典型的AP2结构域特征:3个反向平行的β-折叠和1个α-螺旋。其DNA结合域内有DREB亚族特有的Val~(14)和Ala~(37)氨基酸残基,分别是DREB亚族转录因子与DRE元件和GCC Box元件的结合位点。进化树分析及同源比对分析都表明,该蛋白与拟南芥(Arabidopsis thaliana)中的At1g21910基因所编码的AtAP2和小立碗藓(Physcomitrella patens)中的PpAP2转录因子进化关系最近,属于同一分支,其DNA结合域的一致性达到85%以上。通过Southern blot分析表明该基因在东北红豆杉中以单拷贝形式存在。
通过RACE方法从东北红豆杉中克隆到第二个AP2类转录因子基因TcDREB,其ORF编码410个氨基酸的蛋白,也具有典型的AP2结构域,空间构象及结合的顺式元件类型与TcAP2类似。进化树分析和多重比对表明,就DNA结合域而言,该转录因子与长春花(Catharanthus roseus)的ORCA1和大豆(Glycinemax)的GmDREBa的一致性最高。Southern blot表明该基因在东北红豆杉中也以单拷贝形式存在。
亚细胞定位结果表明两个转录因子都定位在细胞核中。半定量RT-PCR分析显示,它们在幼茎中的表达量最高。RT-QPCR(Real-time Quantitative PCR)表明TcAP2转录水平的表达主要受MeJA和SA、高盐和低温的诱导;而TcDREB的表达受高盐、MeJA和SA以及ABA的诱导。凝胶电泳迁移率实验(Electrophoretic Mobility Shift Assays,EMSA)显示,两个转录因子都能与DRE顺式元件结合。其中TcDREB还能与紫杉醇合成途径中的TS、5α、10β和13α四个关键酶基因的启动子以及TS基因启动子上的GCC Box结合,而突变后的GCCBox不结合TcDREB。进一步用原子力显微镜(Amotic Force Microscope,AFM)证实其结合的特异性,发现加入游离的TcDREB蛋白能使其与DRE元件的结合概率从(19.3±5.2)%下降到(3.9±1.3),而与MDRE的结合概率仅为(4.5±1.6)%,结合成键分布概率验证了TcDREB与DRE元件及四个启动子结合的特异性。综合分析认为,TcDREB与红豆杉异戊二烯代谢产物紫杉醇的合成途径相关,并且这种相关性可能是受ABA信号传导途径调控的。对于TcDREB结合启动子上的顺式元件的具体类型还有待进一步启动子缺失的结合实验证明。
为了探明这两个转录因子在红豆杉中与异戊二烯代谢途径的产物紫杉醇的合成途径的相关性,进一步构建了TcAP2基因和TcDREB基因的植物表达载体并转化拟南芥;同时构建了TS、5α、10β和13α四个关键酶基因的启动子融合gus基因的植物表达载体与转录因子基因植物表达载体共转化拟南芥,已获得转TcDREB基因的阳性植株,为进一步研究其在紫杉醇代谢中的作用打下了基础。
Isoprenoid biosynthetic pathway plays an important role in plant terpenoid natural metabolism pathways and terpenoids' synthesis.Most of the products (Artemisinin,Taxol,Ginkgolide) in isoprenoid biosynthetic pathway have been broadly applied in medical field and clinical therapy for human beings.Therefore, studying the isoprenoid biosynthetic pathway in Taxus cuspidata is important for further understanding the regulation and biosynthesis of taxol,the important anti-cancer chemical produced in Taxus trees.
Taxus is an ancient and precious species.Taxol(paclitaxel) is one of natural diterpenoid alkaloids firstly isolated from the bark of the yew(Taxus brevifolia). Taxol has been well established and approved by FDA as a very important effective chemotherapeutic agent against a wide range of tumors since 1992.However,the supply of Taxol has been limited since the discovery of this natural product,and with increasing applications in chemotherapy,the availability and cost of the drug will remain an important issue.
Transcription factors(TFs),found to be regulators of metabolic flux,have become a powerful tool for the manipulation of complex metabolic engineering in plants.In this thesis,two novel AP2 TF genes,TcAP2 and TcDREB,which may be involved in taxol biosynthetic pathway,has been isolated from Taxus cuspidata for the first time.
Probing into the induction effects of methyl jasmonate(MeJA) and salicylic acid (SA) to taxol content,suspension cells of T.cuspidate were treated with exogenous MeJA and SA before construction of yeast one-hybrid library.Based on spatial distribution frequency of C-13 phenylpropanoid side chain-CoA acyltransferase (BAPT),we explored the optimal induction time of MeJA and SA to the suspension cells for the construction of cDNA library.
The bait plasmid was constructed with cis-element JERE and 72 positive plasmids were selected from T.cuspidata cDNA library by using yeast one-hybrid system.A cDNA-coded TF encoding 268 amino acids was obtained.It had a conserved AP2/EREBP domain in which the N-terminal had a 4-amino acid nuclear localization signal(NLS) and C-terminal had a transcriptional activation domain, named as TcAP2(GenBank accession number EU549860).Multiple alignments and phylogenetic tree analysis indicated that TcAP2 was a member of AP2/EREBP transcription factor gene family and the deduced TcAP2 protein had the highest homology with At1g21910 from Arabidopsis thaliana and PpAP2 from Physcomitrella patens,which possibly reflected the similar functions among them. The binding site V14 inβ-sheet of YRG element and A37 in or-helix of RAYD element was conserved in AP2/EREBP domain which reflected its binding ability to DRE cis-element and GCC Box.
Via SMART Rapid Amplification of 5' and 3'-cDNA Ends(RACE),another novel AP2 transcription factor gene,TcDREB(GenBank accession number EU549861),was cloned from T.cuspidata.The full-length cDNA of TcDREB contained a 1233-bp ORF,encoding 410 amino acids with no N-terminal propeptide. Predicted tertiary structure showed it had the conserved AP2 domain like TcAP2. Multiple alignments showed TcDREB was also a member of the DREB subfamily. TcDREB also possessed threeβ-sheets and oneα-helix as well as V14 and A37 DNA binding sites.
Subcellular location analysis of TcAP2 and TcDREB demonstrated that they were nuclear-localized proteins.Semi-quantitative RT-PCR revealed that TcAP2 and TcDREB constitutively expressed in all test tissues,including stems,roots,and leaves, with high expression in new stems.
EMSA of TcAP2 and TcDREB analysis indicated that both of them could bind to DRE element.Besides,TcDREB could bind to the promoters of 10β,13α,5αand TS as well as GCC Box of TS promoter in taxol biosynthesis pathway but not to MGCC Box.Determination of binding specificity was testified by AFM force measurement. The mean value of single molecular interaction force of TcDREB-DRE was determined as(76.3±2.3) pN.The binding probability was(19.3±5.2).The probabilities of TcDREB and other four promoters were ranged from 15%to 20%, suggesting the regulation of TcDREB in taxol biosynthesis pathway of T.cuspidata.
The expression of TcAP2 and TcDREB could be induced by MeJA,low temperature and salt,ABA and salt,suggesting their involvement in isoprenoid biosynthetic pathway and ABA-dependent signal transduction.
In order to analyze the regulation of the two transcription factors in isoprenoid biosynthetic pathway,the over-expression vector containing TcAP2 or TcDREB cDNAs driven by the CaMV 35S promoters and vectors carrying TS/5α/10β/13α-promoter-GUSA were constructed and single or co-transformed into A. thaliana.Transgenic plants transformed with TcDREB over-expression vector were generated,which will be used to test the effects of stress on electrolyte leakage rate in the transgenic plants in further study.
In summary,the cloning and analysis of TcDREB and promoters of 10β,13α,5αand TS provide the basis for further studying the detailed role of TcDREB in regulation of taxol biosynthetic pathway of T.cuspidata.
引文
Aerts R J,Gisi D,De Carolis E.Methyl jasmonate vapor incresses the developmentally controlled synthesis of alkaloids.Plant J.,1994,5:635-643.
Alex D.,Bach T.J.,Chye M.L.Expression of Brassica juncea 3-hydroxy-3-methylglutaryl CoA synthase is developmentally regulated and stress-responsive.Plant J.,2000,22(5):415-426.
Anne S.,Rithner C.D.,Williams R.M.,Croteau R.B.Molecular cloning of a cytochrome P450 taxane10β-hydroxylase eDNA from Taxus and functional expression in yeast.The Proceedings of the National Academy of Sciences of the United States of America.2001,98(4):1501-1506.
Banni H,Tamada Y,Maruyama O,Nakai K,Miyano S.Extensive feature detection of N-terminal protein sorting signals.2002,18(2):298-305.
Bausells J.Micro-and nano-electromechanical systems for[bio]molecular analysis.Contributions to science,2005,3(1):67-78.
Bea Pauw,Fr(?)d(?)rique A.O.Hilliou,Virginia Sandonis Martin,Guillaume Chatel,Cocky J.F.de Wolf,Antony Champion,Martial Pre,Bert van Duijn,Jan W.Kijne,Leslie van der Fits,and Johan Memelink.Zinc Finger Proteins Act as ranscriptional Repressors of Alkaloid Biosynthesis Genes in Catharanthus roseus.The Journal of Biological Chemistry,2004,279(51):52940-52948.
Bender J,Fink G R.A Myb homologue,ATR1,activates tryptophan gene expression in Arabidopsis.Genetics,1998,95(10):5655-5660.
Bevan M,Bancroft I,Bent E.Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana.Nature,1998,391:485-488.
B.H.Guo1,G.Y.Kai1,H.B.Jin1 and K.X.Tang.Taxol synthesis.African Journal of Biotechnology,2006,5(1):15-20.
Bilaud T,Koering C E,Binet-Brasselet E,Ancelin K,Pollice A,Gasser S M,Gilson E.The telobox,a myb-related telomeric DNA binding motif found in proteins from yeast,plants and human.Nucleic Acids Res.,1996,24:1294-1303.
Boulikas T.Putative nuclear localization signals(NLS) in protein transcription factors.J.Cell Biochem.,1994,55:32-58.
Borevitz J O,Xia Y,Blount J.Activation tagging identifies a conserved MYB regulator of phenypropanoid biosynthesis.Plant Cell,2000,12:2383-2394.
Brown R L,Kazan K,McGrath K C,Maclean D J.A role for the GCC-Box in jasmonate-mediated activation of the PDF1.2 gene of Arabidopsis.Plant Physiology,2003,132(2):1020-1032.
Bruce W,Folkerts O,Gamaat C,Crasta O,Roth B.& Bowen B.Expression profiling of the maize flavonoid pathway genes controlled by estradiol-inducible transcription factors CRC and P.Plant Cell,2000,12:65-79.
Buck M J,Atchley W R.Phylogenetic analysis of plant basic helix-loop-helix proteins.J.Mol.Evol.,2003,56:742-750.
Burbulis IE,Winkel-Shireley B.Interactions among enzymes of the Arabidopsis flavonoid biosynthestic pathway.Proc.Natl.Acad.Sci.USA,1999,96:12929-12934.
Burr F.A.,Burt B.,S.John,et al.Zein storage protein gene family of maize.J Mol Biol,1982,154(32):33-49.
Carey C C,Strahle J.T,Selinger D A,Chandler V L.Mutations in the pale aleurone colorl regulatory gene of the Zea mays anthocyanin pathway have distinct phenotypes relative to the functionally similar TRANSPARENT TESTA GLABRA1gene in Arabidopsis thaliana.Plant Cell,2004,16:450-464.
Capell T,Christou P.Progress in plant metabolic engineering.Current Opinion in Biotechnology,2004,15:148-154.
Chandler V R,Radicella P J,Robbins T P,et al.Two regulatory genes of the maize anthocyanin pathway are homologous:isolation of B utilizing R genomic sequences[J].Plant Cell,1989,1:1175-1183.
Chang C,Shockey J A.The ethylene-reponse pathway:signal perciption to gene regulation.Curr.Opin.Plant.Biol,1999,2(5):352-358.
Chau MD,Croteau R.Molecular cloning and characterization of a cytochrome P450taxoid 2a-hydroxylase involved in Taxol biosynthesis.Arch.Biochem.Biophys.2004a,427:48-57.
Chappell J.Biochemistry and molecular biology of the isoprenoid biosynthetic pathway in plants.A rmu Rev Plant Physiol Plant Mol Biol,1995,46:521-547.
Chen C,Meyermans H,Burggraeve B.Cell-specific and conditional expression of caffeoyl-coenzyme A-3-O-methyltransferase in poplar.Plant Physiol.,2000,123:853-867.
Chen J,Zhu Y,Wang Z Y.Cloning and expression analysis of MYB eDNA in rice.J.Plant Physiol.Mol.Biol.,2002,28(4):267-274.
Chert X.Y.,Xu Z.H.Recent progress in biotechnology and genetic engineering of medicinal plants in China.Medcinal Chemistry Research,1996,6:215-224.
Choi DW,Rodriguez EM,Close TJ.Barley Cbf3 gene identification,expression pattern,and map location.Plant Physiol,2002,129:1781-1787.
Claros MG,Vineens E Computational method to predict mitoehondrially imported proteins and their targeting sequences.Eur.J.Bioehem,1996,241(3):779-786.
Cone K C,Coeeiolone S M,Burr F A.Maize anthocyanin regulatory gene Pl is a duplicate of cl that functions in the plant.Plant Cell,1993,5(12):1795-1805.
Cieza A.,Maier P,Poppel E.The effect of Ginkgo biloba on healthy elderly subjeets.Fortsehritte Der Medzin,2003,1:10-15.
Collu G,Garcia A.A.,van der Heijden R.,Verpoorte R.Activity of the cytoehrome P450 enzyme geraniol 10-hydroxylase and alkaloid production in plant cell cultures.Plant Science.2002,162:165-172
Collua G,Unvera N.,Peltenburg-Loomana A.M.G,van der Heijdena R.,Verpoorte R.,Memelink J.Geraniol 10-hydroxylasel,a cytoehrome P450 enzyme involved in terpenoid indole alkaloid biosynthesis.FEBS Letters,2001,508,215-220.
Cone K C,Burr F A,Burr B.Molecular analysis of the maize anthocyanin regulatory locus C1.Proe.Natl.Acad.Sci.,1983,83:9631-963.
Cone K C,Cocciolone S M,Burr F A,et al.Maize anthocyanin regulatory gene pl is a duplicate of cl that functions in the plant[J].Plant Cell,1993,5:1795-1807.
Croom W.J.,Hagler W.M.,Froetschel M.A.,Johnson A.D.The involvement of slaframine and swainsonine in slobbers syndrome:a review.Journal of Animal Science,1995,73(5):1499-508.
Croteau R.,Kutchan T.M.,Lewis N.G.Biochemistry and molecular biology of plants.American Society of Plant Physiologists,Rockville,MD,2000,1250-1318.
D(?)bora Vom Endt,Jan W.Kijne,Johan Memelink.Transcription factors controlling plant secondary metabolism:what regulates the regulators? Phytochemistry,2002,61:107-114.
de Vetten N,Quattroeehio F,Mol J,et al.The anll locus controlling flower pigmentation in petunia encodes a novel WD-repeat protein conserved in yeast,plants and animals[J]:Genes Dev,1997,11:1422-1434.
Douglas C.Phenylpropaniod metabolism and lignin biosynthesis:from weeds to trees.Trends in plant Sci.,1996,1:171-178.
Dixon R A,Paiva N L.Stress-induced phenylpropanoid metabolism.Plant Cell,1995,7:1085-1097.
Dubouzet JG,Sakuma Y,Ito Y,Kasuga M,Dubouzet EG,Miura S,Seki M,Shinozaki K,Yamaguchi-Shinozaki K.OsDREB genes in rice,Oryza sativa L.,encode transcription activators that function in drought-,high-salt-and cold-responsive gene expression.Plant J.,2003,33:751-763.
Eisenreich W.,Menhard B.,Hylands P.J.,Zenk M.H.,Bather A.Studies on the biosynthesis of taxol:The taxane carbon skeleton is not of mevalonoid origin.The Proceedings of the National Academy of Sciences of the United States of American,1996,93,6431-6436.
Ellis J G,Tokuhisa J G,Llewellyn D J.Does the oca-element occur as a functional component of the promoters of plant genes? Plant J.,1993,4:433-443.
Emanuelsson O.,Nielsen H.,Brunak S.,yon Heijne G,Predicting subcellular localization of proteins based on their N-terminal amino acid sequence.Journal of Molecular Biology.2000,300,1005-1016.
Facchini PJ..Alkaloid biosynthesis in Plants:Biochemistry,cell biology,molecular regulation,and metabolieengineering applications.Annu Rev Plant Physiol,Plant Mol Biol,2001,52:29-66.
Facchini PJ,Huber Allanach KL,Tari LW.Plant aromatic L2amino acid decarboxylases:evolution,biochemistry,regulation,and metabolic engineering applications.Phytochemistry,2000,54(2):121-38.
Facchini PJ,Huber Allanach KL,Tari LW.Plant aromatic L-amino acid decarboxylases:evolution,biochemistry,regulation,and metabolic engineering applications.Phytochemistry,2000,54(2):121-138.
Fan C Z,Purugganan M D,Thomasa D T,Wiegmann B M.Heterogeneous evolution of the Myc-like anthocyanin regulatory gene and its phylogenetic utility in Cornus L.(Cornaceae).Mol.Phyl.Evol.,2004,33:580-594.
Fang Xu,Hong Zhu,Quan-Hong Yao,Isolated and characterization of a cDNA encoding ethylene-responsive element binding protein(EREBP)/AP2-type protein,RCBF2,in Oryza sativa L.Biotechnology Letters,2007,29:165-173.
Finkelstein R R,Lynch T J.The Arabidopsis abscisic acid response gene ABI5encodes a basic leucine zipper transcription factor.Plant Cell,2000,12:599-609.
Florin E L,Moy V T,Gaub H E.Adhesion force between individual Ligand-receptor pairs Science,1994,264:415-417.
ForkmannG,MartensS.Metabolic engineering and applications of flavonoids.Curr Opin Bioteehnol,2001,12:155-160.
Fray R C,Wallace A,Fraser PD.Constitutive expresion of a fruit phytoene 10-synthasegene in transgenic potatoes causes dwarfism by redirecting metabolites from the gibberellin pathway. Plant J., 1995, 8(5): 982-986.
Fried M, Crothers D M. Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis. Nucleic Acids Res., 1981, 9: 6505-6525.
Fritz J, Bailer M K, Lang H P, Rothuizen H, Vettiger P, Meyer E, Giintherodt H J, Gerber Ch, Gimzewski J K. Translating biomolecular recognition into nanomechanics. Science, 2000,288: 316-318.
Garner M M, Revzin A. A gelelectrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system. Nucleic Acids Res., 1981,9: 3047-3060.
Goodrich J, Carpenter R, Coen E S. A common gene regulates pigmentation pattern in diverse plant species [J]. Cell, 1992,68: 955-964.
Gunter Meister, Thomas Tuschl. Mechanisms of gene silencing by double straded RNA. 2004,431:343-349.
Gueritte-Voegelein F, Guenard D, Potier P. Taxol and derivatives, a biogenetic hypothesis, J . Nat. rod., 1987, 50(1): 9-18.
Guillaume Chatel, GreAgory Montiel, Martial PreA, Johan Memelink, Martine Thiersault, Benoit Saint-Pierre, Pierre Doireau and Pascal Gantet. CrMYCl, a Catharanthus roseus elicitor- and jasmonate-responsive bHLH transcription factor that binds the G-box element of the strictosidine synthase gene promoter. Journal of Experimental Botany. 2003, 54(392):2587-2588.
Gu YQ , Yang C , Thara V K, et al. Pit4 is induced by ethylene and salicylic acid , and its product is phosphorylated by the Pto kinase[J ]. Plant Cell, 2000 , 12(5): 771-786.
Gilmour SJ, Zarka DG, Stockinger EJ, Salazar MP, Houghton JM, Thomashow MR Low temperature regulation of ihe Arabidopsis CBF family of AP2 transcriptional activators as an early step in cold-induced COR gene expression. Plant J., 1998, 16:433-442.
Grundlach H , et al . Jasmonic acid is a signal transducer in elicitor - induced plant cell cultures Proc. Nath. Acad. Sci. USA, 1992 , 89 : 2389.
Guerineau F, Benjdia M, Zhou D X. A jasmonate-responsive element within the A. thaliana vspl promoter. Exp. Bot, 2003, 54: 1153-1162.
Gu(?)ritte-Vo(?)gelein F, Guenard D, Potier P. Taxol and derivatives.A biogenetic hypothesis. J Nat Prod, 1987, 50: 9218.
Hahlbrock K, Scheel D. Physiology and molecular biology of phenylpropanoid metabolism. Ann. Rev. Plant Physiol., 1989,40: 347-369.
Hansen K M, Ji H, Wu G, Datar R H, Cote R J. Majumdar A, Thundat T. Cantilever-based optical defection assay for discrimination of DNA single-nucleotide mismatches. Analytical Chemistry, 2001,73:1567-1571.
Hefner J., Ketchum R.E.B., Croteau R. Cloning and Functional Expression of a cDNA Encoding Geranylgeranyl Diphosphate Synthase from Taxus canadensis and Assessment of the Role of this Prenyltransferase in Cells Induced for Taxol Production. Archieves of Biochemistry and Biophysics, 1998,360,62-74.
Heinekamp T, Kuhlmann M, Lenk A. The tobacco bZIP transcription factor BZI-1 binds to G-box elements in the promoters of phenylpropanoid pathway genes in vitro, but it is not involved in their regulation in vivo. Mol. Genet. Genom., 2002, 267(1): 16-26.
He XZ , Dixon RA. Genetic manipulation of isoflvone 7-O-methyl-transferase enhances biosynthesis of 4'-O-methylated isoflavonoid phytoalexins and disease resistance in alfalfa. Plant Cell, 2001,12 :1689-1702.
Ito M. Factors controlling cyclin B expression. Plant Mol. Biol., 2000,43: 677-690.
Jaakola L., Pirttila A. M., Halonen M., Hohtola A. Isolation of high quality RNA from bilberry (Vaccinium myrtillus L.) fruit. Molecular Biotechnology, 2001, 19(2): 201-203.
Jennewein S., Rithner C. D., Williams R. M., Croteau 13-hydroxylase is a cytochrome P450-dependent Proceedings of the National Academy of American. 2001,98(24), 13595-13600.
Jennewein, S., Croteau, R. Taxol, biosynthesis, molecular genetics, and biotechnological applications. Applied Microbiology and Biotechnology. 2001, 57, 13-19.
Jennewein S., Rithner C. D., Williams R. M., Croteau R. Taxol biosynthesis: Taxane 13-hydroxylase is a cytochrome P450-dependent monooxygenase. The Proceedings of the National Academy of Sciences of the United States of American. 2001,98(24), 13595-13600.
Jiang Y X, Zhu C F, Ling L S,.Specific aptamer-protein interaction studied by atomic force microscopy. Anal Chem. 2003, 75: 2112-2116.
John F. Bertram, Robert P. Bolender, Paul D. Sampson. Influence of tissue composition on the final volume of rat liver blocks prepared for electron microscopy.Journal of Electron Microscopy Technique.1986,4(3):303-314.
Johan Memelink,Jan W.Kijne,Robert van der Heijden,Rob Verpoorte.Genetic Modification of Plant Secondary Metabolite Pathways Using Transcriptional Regulators.Advances in Biochemical Engineering Biotechnology.2001,72:103-125.
Johnson C S,Kolevski B,Smyth D R.TRANSPARENT TESTA LABRA2,a trichome and seed coat development gene of Arabidopsis,encodes a WRKY factor.Plant Cell,2002,14(6):1359-1375.
Junping Yu,Shah Sun,Yaxin Jiang,Xinyong Maa,Feng Chen,Guiyou Zhang,Xiaohong Fang.Single molecule study of binding force between transcription factor TINY and its DNA responsive element.Polymer,2006,47:2533-2538.
Kawaoka A,Ebinuma H.Transcriptional control of lignin biosynthesis by tobacco LIM protein.Phytochemistry,2001,57(7):1149-1457.
Kazuko Yamaguchi-Shinozakiaib and Kazuo Shinozaki.A Novel cis-Acting Element in an Arabidopsis Gene is Involved in Responsiveness to Drought,Lowqemperature,or High-Salt Stress.The Plant Cell,1994,6:251-264.
Klayman DL.Qinghaosu(artemisinin):an antimalarial drug from China.Science,1995,228:1049-1055.
Kobayashi S,Ishimaru M,Hiraoka K,et al.Myb-related genes of the Kyoho grape (Vitis labruscana) regulate anthocyanin biosynthesis[J].Planta,2002,215(6):924-933.
Kohler D.R.,Goldspiel B.R.Paclitaxel(taxol).Pharmacotherapy,1994,14(1):3-34.
Kohler J,Goldspiel B R.Evaluation of new drug paclitaxel(Taxol).Pharmacothera y,1994,14:3.
Kims Y,Chung H J,Thomas T L.Isolation of a novel class of bZIP transcription factors that interact with ABAresponsive and em-bryo specification elements in the Dc3 promoter using a modified yeast one-hybrid system.Plant J.,1997,11:1237-1251.
Klimczak L J,Collinge M A,Farini D.Reconstitution of Arabidopsis casein kinase Ⅱfrom recombinant subunits and phophorylation of transcription factor GBF1.Plant Cell,1995,7:105-115.
Kubo H,Peeters A J M,Aarts M G M,et al.ANTHOCYANINLESS2,a homeobox gene affecting anthocyanin distribution and root development in Arabidopsis[J].Plant Cell,1999,11:1217-1226.
Kutchan T.12-O xo-phyvtodienco ic acid accumulation of berberine bridge enzyme t ranscrip t analogous to methyl jasmonate.Journal of Plant Physiology,1991,142:5502-5505.
Kutchan T M.Alkaloid biosynthesis-the basis for metabolic engineering of medicinal plants.Plant ceil,1995,7:1059-1070.
Lansing A.,Haertel M.,Gordon M.,Floss,H.G.Biosynthetic studies on taxol.Planta Medica,1991,57:83.
Lange M.B.,Rujan T.,Martin W.,Croteau R.Isoprenoid biosynthesis,The evolution of two ancient and distinct pathways across genomes.The Proceedings of the National Academy of Sciences of the United States of America.2000,97,13172-13177.
Laskaris G,Bounkhay M,Theodoridis G.Induction of geranylgeranyl diphosphate synthase activity and taxane accumulation in Taxus baccata cell cultures after elicitation by methyl jasmonate.Plant Science,1999,147:1-8.
Lade O.,Furholz A.,Chang H.S.,Zhu T.,Wang X.,Heifetz P.B.,Gruissem W.,Markus L.B.Crosstalk between cytosolic and plastidial pathways of isoprenoid biosynthesis in Arabidopsis thaliana.The Proceedings of the National Academy of Sciences of the United States of America.2003,100:6866-6871.
Lopez-Molina L,Chua N H.A null mutation in a bZIP factor confers ABA-insensitivity in Arabidopsis thaliana.Plant Cell Physiol,2000,41:541-547.
Loyd A M,WalbotV,Davis RW.Arabidopsis and Nicotiana anthocyanin production acivated by maize regulators Rand CI.Science,1992,258:1773-1775.
Lo Y S,Huefner N D,Chan W S,et al.Specific interactions between biotin and avidin studied by atomic force microscopy using Poisson statistical analysis method.Langmuir,1999,15.:743-746.
Lo Y S,Zhu Y J,Beebe Jr T P.Loading-rate dependence of individual ligand-receptor bond-rupture force studied by atomic force microscopy.Langmuir,2001,17:3741-3748.
Lovy W.A.,Robert M.L.,Raymond E.B.K,Christopher D.R.,Williams R.M.,Croteau R.Taxol Biosynthesis-Differential Transformations of Taxadien-5a-ol and Its Acetate Ester by Cytochrome P450 Hydroxylases from Taxus Suspension Cells.Archives of Biochemistry and Biophysics.2001,39:265-278.
Liu Q,Kasuga M,Sakuma Y,Abe H,Miura S,Yamaguchi-Shinozaki K,Shinozaki K.Two transcription factors,DREB1 and DREB2,with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis. Plant Cell .1998,10:1391-1406.
Ludwig SR, Habera LF, Dellaporta SL. Lc, a Member of the Maize R Gene Family Responsible for Tissue-Specific Anthocyanin Production, Encodes a Protein Similar to Transcriptional Activators and Contains the myc-Homology Region. Proceedings of the National Academy of Sciences of the United States of America. 1989, 86(18): 7092-7096.
Magnani E, Sjolander K, Hake S. From endonucleases to transcrip tion factors: evolution of the AP2 DNA binding domain in plants. Plant Cell, 2004, 16: 2265-2277.
Mahmoud SS ,Croteau R. Metabolic engineering of essential oil yield and composition in mint by altering expression of deoxyxylulose phosphate reductoisomerase and menthofuran synthase. Proc Natl Acad Sci USA, 2001 ,98: 8915-8920.
Matton DP, Prescott G, Bertrand C, Camirand A, Brisson N. Identification of cis-acting elements involved in the regulation of the pathogenesis-related gene Sth-2 in potato. Plant Mol.Biol., 1993; 22: 279-291.
Markus L. B., Raymond E. B., Croteau R. B. Metabolite profiling of peppermint oil gland secretory cells and application to herbicide target analysis. Plant Physiology. 2001,127:305-314.
Markus L. B., Rujan T., Martin W., Croteau R. Isoprenoid biosynthesis: The evolution of two ancient and distinct pathways across genomes. The Proceedings of the National Academy of Sciences of the United States of American. 2000, 97: 13172-13177.
Mahmoud SS, Croteau RB. Strategies for transgenic manipulation of monoterpene biosynthesis in plants. Trends Plant Sci., 2002 ,7: 366- 373.
Markus L.B. Crosstalk between cytosolic and plastidial pathways of isoprenoid biosynthesis in Arabidopsis thaliana. The Proceedings of the National Academy of Sciences of the United States of America, 2003,100: 6866-6871.
Mathews H, Clendennen S K, Caldwell C G, et al. Activation tagging in tomato identifies a transcriptional regulator of anthocyanin biosynthesis, modification, and transport [J]. Plant Cell,2003,15:1689-1703.
Meissner R C, Jin H L, Cominelli E. Function search in a large transcription factor gene family in Arabidopsis:assessing the potential of reverse genetics to identify insertional mutations in R2R3 MYB genes.Plant Cell,1999,11:1827-1840.
Memelink J,Verpoorte R,Kijne J W.ORCAnization of jasmonate-responsive gene expression in alkaloid metabolism.Trends Plant Sci.,2001,65:212-219.
Menke F L H,Champion A,Kijne J W,Memelink J.A novel jasmonate-and elicitor-responsive element in the periwinkle secondary metabolite biosynthetic gene STR interacts with a jasmonate-and elicitor-inducible AP2-domain transcription factor.ORCA2.EMBO,1999,18:4455-4463.
Miroslawa F,Kazimierz G,Katarzyna S B,Grazyna Z.Effect of picloram and methyl jasmonate on growth and taxane accumulation in callus of Taxus media var.hatfieldii.Plant Cell Tiss.Org.Cult.,1997,49:75-79.
Moon WJ,Yoo BS,Kim D et al.Elicitation kinetics of taxane production in suspension cultures of Taxus baccata Pendula.Biotechnology Techniques,1998,12(1):79-81.
Nakai K,Kanehisa M.A Knowledge base for predicting protein localization sites in Eukaryotic Cells.1992,14(4):897-911.
Narita JO,Gruissem W.Tomato hydroxymethylglutaryl-CoA reducetase is required early in fruit development but not during repening.Plant Cell,1989,1:181-190.
Nesi N,Debeaujon I,Jond C.The TT8 gene encodes a basic Helix-Loop-Helix domain protein required for expression of DFR and BAN genes in Arabidopsis siliques.Plant Cell,2000,(12):1863-1878.
Nesi N,Debeaujon I,Jond C,et al.The TRANSPARENT TESTA16 locus encodes the ARABIDOPSIS BSISTER MADS domain protein and is required for proper development and pigmentation of the seed coat[J].Plant Cell,2002,14(10):2463-2479.
Nishiyama C,Takahashi K,Ohtake Y.Analysis of transcription region of Eif-1 by using a yeast one-hybrid system.Biosci.Biotechnol.Biochem.,2002,66:1105-1107.
Paz-Ares,J,Ghosal,D,Wienand,U,Peterson,P A,and Saedler,H.The regulatory c7locus of Zea mays encodes a protein with homology to myb proto-oncogene products and with structural similarities to transcriptional activators.EMBO,1987,6:3553-3558.
Palaz(?)n J.,Cusid(?) R.M.,Bonfill M.,Morales C.,Pi(?)ol M.T.Inhibition of paclitaxel and baccatin Ⅲ accumulation by mevinolin and fosmidomycin in suspension cultures of Taxus baccata.Journal of Biotechnology,2003,101:157-163.
Pastuglia M,RuffioChable V,Delorme V,Gaude T,Dumas C,Cock JM.A functional S locus anther gene is not required for the self-incompatibility response in Brassica oleracea.Plant Cell,1997;9(11):2065-2076.
Petroni K,Cominelli E,Consonni G,et al.The tissue specific expression of the maize regulatory gene Hopi determines germination-dependent anthocyanin accumulation[J].Genetics,2000,155:323-336.
Qin F,Li J,Zhang G Y.Isolation and Structural Analysis of DRE-Binding Transcription Factor from Maize(Zea mays L.).Acta Botanica Sinica,2003,45(3):82.
Quattrocchio F,Wing J F,van der Woude K,et al.Analysis of bHLH and MYB-domain proteins:Species-specific regulatory differences are caused by divergent evolution of target anthocyanin genes[J].Plant J,1998,13:475-488.
Quattrocchio F,Wing J,van der Woude K,et al.Molecular analysis of the anthocyanin2 gene of petunia and its role in the evolution of flower color[J].Plant Cell,1999,11:1433-1444.
Rasmussen S,Dixon RA.Transgene2mediated and elicitor-induced perturbation of metabolic channeling at the entry point into the phenylpropanoid pathway.Plant Cell,1999,11:1537-1551.
Reymond P,Kunz B,Paul-Pletzer K,Grimm R,Eckerskom C,Farmer E E.Cloning of a cDNA encoding a plasma membrane-associated,uronide binding phosphoprotein with physical properties similar too viral movement proteins.Plant Cell,1996,8(12):2265-2276.
Riechmann J.L.,Heard J.,Martin G.,et al.Arabidopsis transcription factors:genome-wide comparative analysis among eukaryotes.Science,2000,290(24):2105-2110.
Robin L,Edward E.Farmer.The jasmonate pathway.Science,2002,296:1649-1650.
Roder K,Schweizer M.Running-buffer composition influences DNA-protein complexes detected by electrophoretic mobility-shift assay(EMSA).Biotechnol Appl Biochem,2001,33:209-214.
Sabah Hedhili,Nathalie Giglioli.Regulation of the terpene moiety biosynthesis of Catharanthus roseus terpene indole alkaloids,Phytochem Rev,2007,6:341-351.
Sagasser M,Lu G H,Hahlbrock K.A.thaliana TRANSPARENT TEATA1 is involved in seed coat development and defines the WIP subfamily of plant zine finger proteins.Genes Dev.,2002,16:138-149.
Sakamoto T,Ohmori T,Kageyama K,et al.The purple leaf(P1)locus of rice:the P1(w) allele has a complex organization and includes two genes encoding basic helix-loop-helix proteins involved in anthocyanin biosynthesis[J].Plant Cell Physiol,2001,42(9):982-991.
Sakuma Y,Liu Q,Dubouzet JG.DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs,transcription factors involved in dehydration-and cold-inducible gene expression.Biochemical and Biophysical Research Communications,2002,290(3):998-1009.
Sainz M.B.,Grotewold E.,Chandle V.L.Evidence for direct activation of an anthocyanin promoter by the maize C1 protein and comparison of DNA bind by related Myb domain proteins.Plant Cell,1997,9(3):611-625.
Saitou N,Nei M.The Neighbor-Joining Method-a new method for reconstructing phylogenetic trees.Molecular Biology and Evolution.1987,4(4):406-425.
Yoh Sakuma,Liu Q,Joseph G,et al.DNA-Binding Specificity of the ERF/AP2Domain of Arabidopsis DREBs,Transcription Factors Involved in Dehydration-and Cold-Inducible Gene Expression.Biochemical and Biophysical Research Communications,2002,290:998-1009
Sano H,Seo S,Koizumi N.Regulation by cytokinins of endogenous levels of jasmonic and salicylic acids in mechanically wounded tobacco plants.Plant cell physiol.,1996,37:762-769.
Schoendorf A,Rithner CD,Williams RM,Croteau R.Molecular cloning of a cytochrome P450 taxane 10_-hydroxylase cDNA from Taxus and functional expression in yeast.Proc.Natl.Acad.Sci.USA.2001,98:1501-1506.
Schwechheimer C,Bevan M.The regulation of transcription factor activity in plants.Trend in Plant Science,1998,3(10):278-283.
Schwesinger F,Ros R,Strunz T,et al.Unbinding forces of single antibody-antigen complexes correlate with their thermal.dissociation rates.Proc Natl Acad Sci USA,2000,97:9972-9977.
Sembdner G,Parthier B.The biochemistry and the physiological and molecular action of jasmonates.Ann.Rev.Plant Physiol.Plant Mol.Biol.,1993,44:569-589.
Sepaniak M,Datskos P,Lavrik N,Tipple C.Microcantilever transducers:a new approach in sensor technology.Analytical Chemistry,2002,November:568A-575A.
Shewmaker C K,Sheehy J A,Daley M,Colbum S,Ke D Y.Seed-specific overexpression of phytoene synthase:increase in carotenoids and other metabolic effects.Plant J.,1999,20:401-412.
S.H.Shin,M.C.Shih,W.H.Li.Transcription Factor Families Have Much Higher Expansion Rates in Plants than in Animals.Plant Physiology,2005,139(115):18-26.
Shah,J,Klessig D F.Identification of a salicylic acid-responsive element in the promoter of the tobacco pathogenesis-related 1,3-β-glucanase gene,PR-2d.Plant J.,1996,10(6):1089-1101.
ShinB.,ChoiQ,Yi H,et al.AtMyb21,a gene encodinga flower-specific tanscription factor,is regulated by COP1.The Plant Journal,2002,30(5):23-32.
Soave C.,Tardani L.,Fonzo N.,et al.Zein level in maize endosperm depends on a protein under control of the opaque-2 and opaque-6 loci.Ce11,1981,27(12):403-410.
Spelt C,Quattrocchio F,Mol J,et al.Anthocyaninl of petunia encodes a basic-helix loop helix protein that directly activates structural anthocyanin genes[J].Plant Cell,2000,12:1619-1631.
Stark D M,Timmerman K P,Barry G F,Preiss J & Kishore G M.Regulation of the amount of starch in plant tissue by ADP glucose pyrophosphorylase,Science,1992,258:287-292.
Tang Z,Zhao Y,Mei E Molecular cloning and characterization of a human gene involved in transcriptional regulation of hTERT.Biochem-Biophys-Res-Commun,2004,324(4):1324.
Thompson JD,Higgins DG,Gibson TJ.Clustal-W-Improving the sensitivity of progressinve multiple sequence alignment through sequence weighting,position-specific gap penalities and weight matrix choice.Nucleic Acids Res.,1994,22(22):4673-4680.
Tonelli C,Consonni G,Faccio D S,et al.Genetic and molecular analysis of Sn,a light-inducible,tissue specific regulatory gene in maize[J].Mol Gen Genet,1991,225:401-410.
van der Fits L.,Memelink J.ORCA3,a Jasmonate-Responsive Transcriptional Regulator of Plant Primary and Secondary Metabolism.Science,2000,289:.295-297.
Vincentz-Carbajosa J,Moose S P,Parsons R L.A maize zinc-finger protein binds the prolamin box in zein gene promoters and interacts with the basic leucine zipper transcriptional activator Opaque2.Proc.Natl.Acad.Sci.USA,1997,94:7685-7690.
Ramsay N A,Glover B J.MYB-bHLH-WD40 protein complex and the evolution of cdlular diversity.Trends Plant Sci.,2005,10(2):63-70.
Richter S,Lamppa GK.Determinants for removal and degradation of transit peptids of chloroplast precursor protein.J.Biol.Chem.,2002,277(46):43888-43894.
Riechmann J.L.,Heard J.,Martin G,et al.Arabidopsis transcription factors:genome-wide comparative analysis among eukaryotes.Seience,2000,290(24):2105-2110.
Rongmin Chen,Zhongfu Ni,Xiuling Nie,Yuxiang Qin,Guoqing Dong,Qixin Sun.Isolation and characterization of genes encoding Myb transcription factor in wheat(Triticum aestivem L.).Plant Science 169(2005) 1146-1154.
Ros R,Schwesinger F,Anselmetti D,Kubon M,Sehafer R,Pliickthun A,Tiefenauer L.Antigen binding,forces of individually addressed single-chain Fv antibody molecules.Proc.Natl.Acad.Sci.,1998,95:7402-7405.
Walker A R.Davison P A,Bolognesi-Winfield A C,et al.The TRANSPARENT TESTA(LA BRA I locus,which regulates trichome differentiation and anthocyanin biosynthesis in Araidopsis,encodes a WD40 repeat protein[J].Plant Cell,1999,11:1337-1350.
Walker K.,Croteau R.Taxol biosynthesis:Molecular cloning of a benzoyl-CoA:taxane 2a-O-benzoyltransferase cDNA from Taxus and functional expression in Escherichia coli.The Proceedings of the National Academy of Sciences of the United States of American.2000,97:99164-6340.
Walker K.,Fujisaki S.,Long R.,Croteau R.The final acylation step in Taxol biosynthesis:Cloning of the taxoid C13-side-chain N-benzoyltransferase from Taxus.The Proceedings of the National Academy of Sciences of the United States of American,2002,99:9166-9171.
Walker K.,Croteau R.Molecular cloning of a 10-deacetylbaccatin Ⅲ-10-O-acetyl transferase cDNA from Taxus and functional expression in Escherichia coli.The Proceedings of the National Academy of Sciences of the United States of America,2000,97(2),583-587.
WalkerK.,Croteau R.Taxol biosynthesis:Molecular cloning of a benzoyl-CoA:taxane 2α-O-benzoyltransferase cDNA from Taxus and functional expression in Escherichia coli.The Proceedings of the National Academy of Sciences of the United States of American,2000,97:99164-6340.
Walker K.,Fujisaki S.,Long R.,and Croteau R.Molecular cloning and heterologous expression of the C-13 phenylpropanoid side chain-CoA acyltransferase that functions in Taxol biosynthesis.The Proceedings of the National Academy of Sciences of the United States of American,2002,99,12715-12720.
Wang Y.D.,Yuan Y.J.,Wu J.C.Translocation of isopentenyl pyrophosphate for Taxol biosynthesis in suspension cultures of Taxus chinensis var.mairei.Plant Cell,Tissue and Organ Culture,2003,74,283-288.
Wani M.C.,Taylor H.L.,Wall M.E.,Coggon P.,McPhail A.T.Plant antitumor agents.The isolation and structure of taxol,a novel antileukemic and antitumor agent from Taxus brevifolia.Journal of American Chemical Society.1971,93,2325-2327.
Weisshaar B,Armstrong G A,Block A,et al.Light-inducible and constitutively expressed DNA-binding proteins recognizing a plant promoter element with functional relevance in light responsiveness[J].EMBO J,1991,10(7):1777-1786.
Wheeler A.L.,Long R.M.,Ketchum R.E.B.,Rithner C.D.,Williams R.M.,Croteau R.Taxol Biosynthesis:Differential Transformations of Taxadien-5α-ol and Its Acetate Ester by Cytochrome P450 Hydroxylases from Taxus Suspension Cells.Biochimica et Biophysica Acta.2001,390(2):265-278.
Wildung M.R.,Croteau R.A.cDNA cloing for taxadiene synthase that catalyzes the committed step of Taxol biosynthesis.J.Biol.Chem.1996,271:9201-9204.
Willner I.Nanoparticle-and nanorod-biomaterial hybrid system for sensor,circuitry and motor applications.Contributions to Science,2005,3(1):79-91.
Wu G,Ji H F,Hansen K,Thundat T,Datar R,Cote R,Hagan M F,Chakraborty A K,Majumdar A.Origin of nanomechanical cantilever motion generated from biomolecular interactions.Proc.Natl.Acad.Sci.,2001,98:1560-1564.
XueB.,CharestPJ.,Devantier Y,et al.Characterization of a MYBR2R3 gene from black spruce(Picea mariana)that shares functional conservation with maize C1.Mot.Genet.Genomics.,2003,270(35):78-86.
Xue G P.Characterization of the DNA-binding profile of barley HvCBF1 using an enzymatic method for rapid,quantitative and high-throughput analysis of the DNA-binding activity.Nucleic Acids Res.,2002,30:77.
Xue-Ping Li,Ai-Guo Tian,uang-Zuo Luo,Zhi-Zhong Gong,Jin-Song Zhang,Shou-Yi Chen.Soybean RE-binding transcription factors that are responsive to abiotic stresses.Theor Appl Genet,2005,110:1355-1362.
Ye X,Al-Babili S,Kloti A,Zhang J,Lucca P,Potrykus I.Engineering the provitamin A(beta-carotene) biosynthetic pathway into(carotenoid-free) rice endosperm.Science,2000,287:303-305.
Yazaki K,Takeda K,Tabata M.Effects of methyl jasmonate on shikonin and dihydroechinofuran production in Lithospermum cell cultures.Plant Cell Physiol.,1997,38:776-782.
Zamir L.O.,Nedea M.E.,Belair S.,Sauriol F.,Mamer O.,jacqmain E.,Jean F.I.,Garneau F.X.Biosynthetic building blocks of Taxus canadiensis taxanes.Tetrahedron Letters,1992,33,5235-5236.
Zhang L,Ding R X,Chai Y R.Engineering tropane biosynthetic pathway in Hyoscyamus niger hairy root cultures.Proc.Natl.Acad.Sci.USA,2004,101:6786-6791.
Zlatanova J,Lindsay S M,Leuba S H.Single molecule force spectroscopy in biology using the atomic force microscope.Prog Bioph Mole Biol,2000,74:37-61.
Zhou J M,Tang X Y,Martin GB.The Pto kinase coffering resistance to tomato bacterial speck disease interacts with proteins that bind a cis-element of pathogenesis2related genes[J].EMBO J,1997,16:3207-3218.
Zhulidov P A,Bogdanova E A,Shcheglov A S,Vagner L L.Simple cDNA normalization using kamchatka crab duplex-specific nuclease.Nucleic Acid.Res.,2004,32:37.
曹泽或,黄本开,王庆亚,宣伟,凌腾芳,张博,陈曦,聂理,沈文飚.血红素加氧酶催化产生的NO参与ABA诱导的蚕豆气孔关闭及其信号转导.科学通报,2007,52(15):1771-1778.
蔡昆争,董桃杏,徐涛.茉莉酸类物质(JAs)的生理特性及其在逆境胁迫中的抗性作用.生态环境,2006,15(2):397-404.
陈峰,余军平,方晓红,张贵友.鉴定水稻中一个新的专一结合GCC元件的AP2/EREBP族转录因子.生物化学与生物物理进展,2006,33(7):627-634.
陈晓亚,刘培.植物次生代谢的分子生物学及基因工程.生命科学,1996,8(2):8-11.
戴大临著.生物医学电镜样品制备方法.天津大学出版社.1993.
耿海波,安丽平,陈力,刘洁.抗癌药物喜树碱及其衍生物的研究进展.河北化工,2007,30(3):13-15.
杜丽娜,张存莉,朱玮,张高宏.植物次生代谢合成途径及生物学意义.西北林 学院学报,2005,20(3):150-155.
韩梅,赵博,安志刚,ThomasRausch,祖元刚.长春花萜类吲哚生物碱生物合成途径中重要酶(DXR、SLS、G10H、STR)基因的克隆与表达.植物研究,2001,27(5):564-568.
韩志萍,安利佳,侯和胜.AP2/EREBP转录因子的结构与功能.中国农学通报.2006,22(2):33-38.
何水林.参与植物次生代谢调控的转录因子及其在植物次生代谢遗传改良中的应用.热带亚热带植物学报,2004,12(4):374-380.
何水林,郑金贵,王晓峰,王燕华,许明,李斌莲,林明.植物次生代谢:功能、调控及其基因工程.应用与环境生物学报,2002,8(5):558-563.
李丽娟,赵奂,李艳军,刘祥林,何奕昆.植物中的DREB类转录因子.首都师范大学学报(自然科学版),2006,27(5):61-67.
李鹏,宁熙平,吴鸿.紫锥菊药用部位的结构及其与多酚类化合物积累的关系.中草药,2007,38(4):606-610
廖名湘,方福德.酵母单杂交体系-一种研究DNA-蛋白相互作用的有效方法.中国医学科学院学报,2000,22:388-391.
廖志华.紫杉醇前体生物合成的分子生物学和抗癌萜类吲哚生物碱的代谢工程,2005.博士学位论文.
刘强,张贵友,陈受益.植物转录因子的结构与调控作用.科学通报.2000,45(2):1465-147.
梅兴国,张舟宁,苏湘鄂,龚伟.水杨酸对红豆杉细胞的诱导作用.生物技术,2000,10(6):18-20.
秦峰,江雅新,马新勇,陈峰,方晓红,白春礼,李一勤.原子力显微镜(AFM)研究转录因子与DNA顺式作用元件间的特异性相互作用力.科学通报,2004,49(12):1157-1161.
秦秋琳 银杏中与木质素合成和苯丙氨酸代谢相关的转录调控因子的克隆与研究.2007.博士学位论文.
施中东,未作君,元英进.南方红豆杉细胞培养合成紫杉醇诱导子浓度的优化.天然产物研究与开发,2000,12(4):36-40.
苏湘鄂,梅兴国,龚伟,张舟宁.茉莉酸甲酯与真菌诱导物、水杨酸组合对红豆杉细胞中紫杉醇含量的影响.生物技术,2001,11(1):10-13.
谢永丽.一类植物中特有的转录因子—AP2/EREBP转录因子家族.青海师范大学学报(自然科学版),2006,(3):80-83.
杨致荣,毛雪,李润植.植物次生代谢基因工程研究进展.植物生理与分子生物 学学报.植物生理与分子生物学学报,2005,31(1):11-18.
王鹏.通过RNA介导的基因抑制对烟碱代谢调控的研究.2007.博士学位论文
吴大利等:干扰RNA(RNAi)作用机理及其在植物细胞工程中的应用进展,2006,26(9):84-90.
郑清平,余龙江,刘智,李默怡,向福,杨秦.红豆杉细胞非甲羟戊酸途径关键酶基因dxr的克隆与分析.生物工程学报,2004,20(4):548-553.
周忠强,梅兴国.水杨酸对细胞培养生产紫杉烷的影响.天然产物研究与开发,2002,14(2):11-13.
张剑峰,郭志刚.Effect of Methyl Jasmortic Acid on Baccatin Ⅲ Biosynthesis.清华大学学报(自然科学版)(英文版),2006,11(3):363-367.
张淼,赵畅,李法莲,邵群.具有AP2结构域的转录因子家族的研究进展.科技信息(学术研究),2007,(21):332-335.
张珏,吕加国;朱驹.抗肿瘤药物紫杉醇的化学研究进展.中国新药杂志,2006,15(3):178-181.
赵锋,胡鸢雷;祝建波;林忠平.厚叶悬蒴苣苔BcWRKY1基因启动子克隆及功能元件分析.生物技术通报,2007,(2):128-131.
朱佳.玉米籽粒中的转录因子研究.2007.硕士学位论文.
宗成文,章镇,房经贵,陶建敏,赵密珍.葡萄LEAFY基因启动子的克隆与序列分析.南京农业大学学报,2007,30(4):20-25.
金冬雁、黎孟枫等译,侯云德等校.分子克隆实验指南(第二版).科学出版社,1996年5月出版.
Alex D.,Bach T.J.,Chye M.L.Expression of Brassica juncea 3-hydroxy-3-methylglutaryl CoA synthase is developmentally regulated and stress-responsive.Plant J.,2000,22(5):415-426.
Anne S.,Rithner C.D.,Williams R.M.,Croteau R.B.Molecular cloning of a cytochrome P450 taxane10β-hydroxylase eDNA from Taxus and functional expression in yeast.The Proceedings of the National Academy of Sciences of the United States of America.2001,98(4):1501-1506.
Banni H,Tamada Y,Maruyama O,Nakai K,Miyano S.Extensive feature detection of N-terminal protein sorting signals.2002,18(2):298-305.
Bausells J.Micro-and nano-electromechanical systems for[bio]molecular analysis.Contributions to science,2005,3(1):67-78.
Bea Pauw,Fr(?)d(?)rique A.O.Hilliou,Virginia Sandonis Martin,Guillaume Chatel,Cocky J.F.de Wolf,Antony Champion,Martial Pre,Bert van Duijn,Jan W.Kijne,Leslie van der Fits,and Johan Memelink.Zinc Finger Proteins Act as ranscriptional Repressors of Alkaloid Biosynthesis Genes in Catharanthus roseus.The Journal of Biological Chemistry,2004,279(51):52940-52948.
Bender J,Fink G R.A Myb homologue,ATR1,activates tryptophan gene expression in Arabidopsis.Genetics,1998,95(10):5655-5660.
Bevan M,Bancroft I,Bent E.Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana.Nature,1998,391:485-488.
B.H.Guo1,G.Y.Kai1,H.B.Jin1 and K.X.Tang.Taxol synthesis.African Journal of Biotechnology,2006,5(1):15-20.
Bilaud T,Koering C E,Binet-Brasselet E,Ancelin K,Pollice A,Gasser S M,Gilson E.The telobox,a myb-related telomeric DNA binding motif found in proteins from yeast,plants and human.Nucleic Acids Res.,1996,24:1294-1303.
Boulikas T.Putative nuclear localization signals(NLS) in protein transcription factors.J.Cell Biochem.,1994,55:32-58.
Borevitz J O,Xia Y,Blount J.Activation tagging identifies a conserved MYB regulator of phenypropanoid biosynthesis.Plant Cell,2000,12:2383-2394.
Brown R L,Kazan K,McGrath K C,Maclean D J.A role for the GCC-Box in jasmonate-mediated activation of the PDF1.2 gene of Arabidopsis.Plant Physiology,2003,132(2):1020-1032.
Bruce W,Folkerts O,Gamaat C,Crasta O,Roth B.& Bowen B.Expression profiling of the maize flavonoid pathway genes controlled by estradiol-inducible transcription factors CRC and P.Plant Cell,2000,12:65-79.
Buck M J,Atchley W R.Phylogenetic analysis of plant basic helix-loop-helix proteins.J.Mol.Evol.,2003,56:742-750.
Burbulis IE,Winkel-Shireley B.Interactions among enzymes of the Arabidopsis flavonoid biosynthestic pathway.Proc.Natl.Acad.Sci.USA,1999,96:12929-12934.
Burr F.A.,Burt B.,S.John,et al.Zein storage protein gene family of maize.J Mol Biol,1982,154(32):33-49.
Carey C C,Strahle J.T,Selinger D A,Chandler V L.Mutations in the pale aleurone colorl regulatory gene of the Zea mays anthocyanin pathway have distinct phenotypes relative to the functionally similar TRANSPARENT TESTA GLABRA1gene in Arabidopsis thaliana.Plant Cell,2004,16:450-464.
Capell T,Christou P.Progress in plant metabolic engineering.Current Opinion in Biotechnology,2004,15:148-154.
Chandler V R,Radicella P J,Robbins T P,et al.Two regulatory genes of the maize anthocyanin pathway are homologous:isolation of B utilizing R genomic sequences[J].Plant Cell,1989,1:1175-1183.
Chang C,Shockey J A.The ethylene-reponse pathway:signal perciption to gene regulation.Curr.Opin.Plant.Biol,1999,2(5):352-358.
Chau MD,Croteau R.Molecular cloning and characterization of a cytochrome P450taxoid 2a-hydroxylase involved in Taxol biosynthesis.Arch.Biochem.Biophys.2004a,427:48-57.
Chappell J.Biochemistry and molecular biology of the isoprenoid biosynthetic pathway in plants.A rmu Rev Plant Physiol Plant Mol Biol,1995,46:521-547.
Chen C,Meyermans H,Burggraeve B.Cell-specific and conditional expression of caffeoyl-coenzyme A-3-O-methyltransferase in poplar.Plant Physiol.,2000,123:853-867.
Chen J,Zhu Y,Wang Z Y.Cloning and expression analysis of MYB eDNA in rice.J.Plant Physiol.Mol.Biol.,2002,28(4):267-274.
Chert X.Y.,Xu Z.H.Recent progress in biotechnology and genetic engineering of medicinal plants in China.Medcinal Chemistry Research,1996,6:215-224.
Choi DW,Rodriguez EM,Close TJ.Barley Cbf3 gene identification,expression pattern,and map location.Plant Physiol,2002,129:1781-1787.
Claros MG,Vineens E Computational method to predict mitoehondrially imported proteins and their targeting sequences.Eur.J.Bioehem,1996,241(3):779-786.
Cone K C,Coeeiolone S M,Burr F A.Maize anthocyanin regulatory gene Pl is a duplicate of cl that functions in the plant.Plant Cell,1993,5(12):1795-1805.
Cieza A.,Maier P,Poppel E.The effect of Ginkgo biloba on healthy elderly subjeets.Fortsehritte Der Medzin,2003,1:10-15.
Collu G,Garcia A.A.,van der Heijden R.,Verpoorte R.Activity of the cytoehrome P450 enzyme geraniol 10-hydroxylase and alkaloid production in plant cell cultures.Plant Science.2002,162:165-172
Collua G,Unvera N.,Peltenburg-Loomana A.M.G,van der Heijdena R.,Verpoorte R.,Memelink J.Geraniol 10-hydroxylasel,a cytoehrome P450 enzyme involved in terpenoid indole alkaloid biosynthesis.FEBS Letters,2001,508,215-220.
Cone K C,Burr F A,Burr B.Molecular analysis of the maize anthocyanin regulatory locus C1.Proe.Natl.Acad.Sci.,1983,83:9631-963.
Cone K C,Cocciolone S M,Burr F A,et al.Maize anthocyanin regulatory gene pl is a duplicate of cl that functions in the plant[J].Plant Cell,1993,5:1795-1807.
Croom W.J.,Hagler W.M.,Froetschel M.A.,Johnson A.D.The involvement of slaframine and swainsonine in slobbers syndrome:a review.Journal of Animal Science,1995,73(5):1499-508.
Croteau R.,Kutchan T.M.,Lewis N.G.Biochemistry and molecular biology of plants.American Society of Plant Physiologists,Rockville,MD,2000,1250-1318.
D(?)bora Vom Endt,Jan W.Kijne,Johan Memelink.Transcription factors controlling plant secondary metabolism:what regulates the regulators? Phytochemistry,2002,61:107-114.
de Vetten N,Quattroeehio F,Mol J,et al.The anll locus controlling flower pigmentation in petunia encodes a novel WD-repeat protein conserved in yeast,plants and animals[J]:Genes Dev,1997,11:1422-1434.
Douglas C.Phenylpropaniod metabolism and lignin biosynthesis:from weeds to trees.Trends in plant Sci.,1996,1:171-178.
Dixon R A,Paiva N L.Stress-induced phenylpropanoid metabolism.Plant Cell,1995,7:1085-1097.
Dubouzet JG,Sakuma Y,Ito Y,Kasuga M,Dubouzet EG,Miura S,Seki M,Shinozaki K,Yamaguchi-Shinozaki K.OsDREB genes in rice,Oryza sativa L.,encode transcription activators that function in drought-,high-salt-and cold-responsive gene expression.Plant J.,2003,33:751-763.
Eisenreich W.,Menhard B.,Hylands P.J.,Zenk M.H.,Bather A.Studies on the biosynthesis of taxol:The taxane carbon skeleton is not of mevalonoid origin.The Proceedings of the National Academy of Sciences of the United States of American,1996,93,6431-6436.
Ellis J G,Tokuhisa J G,Llewellyn D J.Does the oca-element occur as a functional component of the promoters of plant genes? Plant J.,1993,4:433-443.
Emanuelsson O.,Nielsen H.,Brunak S.,yon Heijne G,Predicting subcellular localization of proteins based on their N-terminal amino acid sequence.Journal of Molecular Biology.2000,300,1005-1016.
Facchini PJ..Alkaloid biosynthesis in Plants:Biochemistry,cell biology,molecular regulation,and metabolieengineering applications.Annu Rev Plant Physiol,Plant Mol Biol,2001,52:29-66.
Facchini PJ,Huber Allanach KL,Tari LW.Plant aromatic L2amino acid decarboxylases:evolution,biochemistry,regulation,and metabolic engineering applications.Phytochemistry,2000,54(2):121-38.
Facchini PJ,Huber Allanach KL,Tari LW.Plant aromatic L-amino acid decarboxylases:evolution,biochemistry,regulation,and metabolic engineering applications.Phytochemistry,2000,54(2):121-138.
Fan C Z,Purugganan M D,Thomasa D T,Wiegmann B M.Heterogeneous evolution of the Myc-like anthocyanin regulatory gene and its phylogenetic utility in Cornus L.(Cornaceae).Mol.Phyl.Evol.,2004,33:580-594.
Fang Xu,Hong Zhu,Quan-Hong Yao,Isolated and characterization of a cDNA encoding ethylene-responsive element binding protein(EREBP)/AP2-type protein,RCBF2,in Oryza sativa L.Biotechnology Letters,2007,29:165-173.
Finkelstein R R,Lynch T J.The Arabidopsis abscisic acid response gene ABI5encodes a basic leucine zipper transcription factor.Plant Cell,2000,12:599-609.
Florin E L,Moy V T,Gaub H E.Adhesion force between individual Ligand-receptor pairs Science,1994,264:415-417.
ForkmannG,MartensS.Metabolic engineering and applications of flavonoids.Curr Opin Bioteehnol,2001,12:155-160.
Fray R C,Wallace A,Fraser PD.Constitutive expresion of a fruit phytoene 10-synthasegene in transgenic potatoes causes dwarfism by redirecting metabolites from the gibberellin pathway. Plant J., 1995, 8(5): 982-986.
Fried M, Crothers D M. Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis. Nucleic Acids Res., 1981, 9: 6505-6525.
Fritz J, Bailer M K, Lang H P, Rothuizen H, Vettiger P, Meyer E, Giintherodt H J, Gerber Ch, Gimzewski J K. Translating biomolecular recognition into nanomechanics. Science, 2000,288: 316-318.
Garner M M, Revzin A. A gelelectrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system. Nucleic Acids Res., 1981,9: 3047-3060.
Goodrich J, Carpenter R, Coen E S. A common gene regulates pigmentation pattern in diverse plant species [J]. Cell, 1992,68: 955-964.
Gunter Meister, Thomas Tuschl. Mechanisms of gene silencing by double straded RNA. 2004,431:343-349.
Gueritte-Voegelein F, Guenard D, Potier P. Taxol and derivatives, a biogenetic hypothesis, J . Nat. rod., 1987, 50(1): 9-18.
Guillaume Chatel, GreAgory Montiel, Martial PreA, Johan Memelink, Martine Thiersault, Benoit Saint-Pierre, Pierre Doireau and Pascal Gantet. CrMYCl, a Catharanthus roseus elicitor- and jasmonate-responsive bHLH transcription factor that binds the G-box element of the strictosidine synthase gene promoter. Journal of Experimental Botany. 2003, 54(392):2587-2588.
Gu YQ , Yang C , Thara V K, et al. Pit4 is induced by ethylene and salicylic acid , and its product is phosphorylated by the Pto kinase[J ]. Plant Cell, 2000 , 12(5): 771-786.
Gilmour SJ, Zarka DG, Stockinger EJ, Salazar MP, Houghton JM, Thomashow MR Low temperature regulation of ihe Arabidopsis CBF family of AP2 transcriptional activators as an early step in cold-induced COR gene expression. Plant J., 1998, 16:433-442.
Grundlach H , et al . Jasmonic acid is a signal transducer in elicitor - induced plant cell cultures Proc. Nath. Acad. Sci. USA, 1992 , 89 : 2389.
Guerineau F, Benjdia M, Zhou D X. A jasmonate-responsive element within the A. thaliana vspl promoter. Exp. Bot, 2003, 54: 1153-1162.
Gu(?)ritte-Vo(?)gelein F, Guenard D, Potier P. Taxol and derivatives.A biogenetic hypothesis. J Nat Prod, 1987, 50: 9218.
Hahlbrock K, Scheel D. Physiology and molecular biology of phenylpropanoid metabolism. Ann. Rev. Plant Physiol., 1989,40: 347-369.
Hansen K M, Ji H, Wu G, Datar R H, Cote R J. Majumdar A, Thundat T. Cantilever-based optical defection assay for discrimination of DNA single-nucleotide mismatches. Analytical Chemistry, 2001,73:1567-1571.
Hefner J., Ketchum R.E.B., Croteau R. Cloning and Functional Expression of a cDNA Encoding Geranylgeranyl Diphosphate Synthase from Taxus canadensis and Assessment of the Role of this Prenyltransferase in Cells Induced for Taxol Production. Archieves of Biochemistry and Biophysics, 1998,360,62-74.
Heinekamp T, Kuhlmann M, Lenk A. The tobacco bZIP transcription factor BZI-1 binds to G-box elements in the promoters of phenylpropanoid pathway genes in vitro, but it is not involved in their regulation in vivo. Mol. Genet. Genom., 2002, 267(1): 16-26.
He XZ , Dixon RA. Genetic manipulation of isoflvone 7-O-methyl-transferase enhances biosynthesis of 4'-O-methylated isoflavonoid phytoalexins and disease resistance in alfalfa. Plant Cell, 2001,12 :1689-1702.
Ito M. Factors controlling cyclin B expression. Plant Mol. Biol., 2000,43: 677-690.
Jaakola L., Pirttila A. M., Halonen M., Hohtola A. Isolation of high quality RNA from bilberry (Vaccinium myrtillus L.) fruit. Molecular Biotechnology, 2001, 19(2): 201-203.
Jennewein S., Rithner C. D., Williams R. M., Croteau 13-hydroxylase is a cytochrome P450-dependent Proceedings of the National Academy of American. 2001,98(24), 13595-13600.
Jennewein, S., Croteau, R. Taxol, biosynthesis, molecular genetics, and biotechnological applications. Applied Microbiology and Biotechnology. 2001, 57, 13-19.
Jennewein S., Rithner C. D., Williams R. M., Croteau R. Taxol biosynthesis: Taxane 13-hydroxylase is a cytochrome P450-dependent monooxygenase. The Proceedings of the National Academy of Sciences of the United States of American. 2001,98(24), 13595-13600.
Jiang Y X, Zhu C F, Ling L S,.Specific aptamer-protein interaction studied by atomic force microscopy. Anal Chem. 2003, 75: 2112-2116.
John F. Bertram, Robert P. Bolender, Paul D. Sampson. Influence of tissue composition on the final volume of rat liver blocks prepared for electron microscopy.Journal of Electron Microscopy Technique.1986,4(3):303-314.
Johan Memelink,Jan W.Kijne,Robert van der Heijden,Rob Verpoorte.Genetic Modification of Plant Secondary Metabolite Pathways Using Transcriptional Regulators.Advances in Biochemical Engineering Biotechnology.2001,72:103-125.
Johnson C S,Kolevski B,Smyth D R.TRANSPARENT TESTA LABRA2,a trichome and seed coat development gene of Arabidopsis,encodes a WRKY factor.Plant Cell,2002,14(6):1359-1375.
Junping Yu,Shah Sun,Yaxin Jiang,Xinyong Maa,Feng Chen,Guiyou Zhang,Xiaohong Fang.Single molecule study of binding force between transcription factor TINY and its DNA responsive element.Polymer,2006,47:2533-2538.
Kawaoka A,Ebinuma H.Transcriptional control of lignin biosynthesis by tobacco LIM protein.Phytochemistry,2001,57(7):1149-1457.
Kazuko Yamaguchi-Shinozakiaib and Kazuo Shinozaki.A Novel cis-Acting Element in an Arabidopsis Gene is Involved in Responsiveness to Drought,Lowqemperature,or High-Salt Stress.The Plant Cell,1994,6:251-264.
Klayman DL.Qinghaosu(artemisinin):an antimalarial drug from China.Science,1995,228:1049-1055.
Kobayashi S,Ishimaru M,Hiraoka K,et al.Myb-related genes of the Kyoho grape (Vitis labruscana) regulate anthocyanin biosynthesis[J].Planta,2002,215(6):924-933.
Kohler D.R.,Goldspiel B.R.Paclitaxel(taxol).Pharmacotherapy,1994,14(1):3-34.
Kohler J,Goldspiel B R.Evaluation of new drug paclitaxel(Taxol).Pharmacothera y,1994,14:3.
Kims Y,Chung H J,Thomas T L.Isolation of a novel class of bZIP transcription factors that interact with ABAresponsive and em-bryo specification elements in the Dc3 promoter using a modified yeast one-hybrid system.Plant J.,1997,11:1237-1251.
Klimczak L J,Collinge M A,Farini D.Reconstitution of Arabidopsis casein kinase Ⅱfrom recombinant subunits and phophorylation of transcription factor GBF1.Plant Cell,1995,7:105-115.
Kubo H,Peeters A J M,Aarts M G M,et al.ANTHOCYANINLESS2,a homeobox gene affecting anthocyanin distribution and root development in Arabidopsis[J].Plant Cell,1999,11:1217-1226.
Kutchan T.12-O xo-phyvtodienco ic acid accumulation of berberine bridge enzyme t ranscrip t analogous to methyl jasmonate.Journal of Plant Physiology,1991,142:5502-5505.
Kutchan T M.Alkaloid biosynthesis-the basis for metabolic engineering of medicinal plants.Plant ceil,1995,7:1059-1070.
Lansing A.,Haertel M.,Gordon M.,Floss,H.G.Biosynthetic studies on taxol.Planta Medica,1991,57:83.
Lange M.B.,Rujan T.,Martin W.,Croteau R.Isoprenoid biosynthesis,The evolution of two ancient and distinct pathways across genomes.The Proceedings of the National Academy of Sciences of the United States of America.2000,97,13172-13177.
Laskaris G,Bounkhay M,Theodoridis G.Induction of geranylgeranyl diphosphate synthase activity and taxane accumulation in Taxus baccata cell cultures after elicitation by methyl jasmonate.Plant Science,1999,147:1-8.
Lade O.,Furholz A.,Chang H.S.,Zhu T.,Wang X.,Heifetz P.B.,Gruissem W.,Markus L.B.Crosstalk between cytosolic and plastidial pathways of isoprenoid biosynthesis in Arabidopsis thaliana.The Proceedings of the National Academy of Sciences of the United States of America.2003,100:6866-6871.
Lopez-Molina L,Chua N H.A null mutation in a bZIP factor confers ABA-insensitivity in Arabidopsis thaliana.Plant Cell Physiol,2000,41:541-547.
Loyd A M,WalbotV,Davis RW.Arabidopsis and Nicotiana anthocyanin production acivated by maize regulators Rand CI.Science,1992,258:1773-1775.
Lo Y S,Huefner N D,Chan W S,et al.Specific interactions between biotin and avidin studied by atomic force microscopy using Poisson statistical analysis method.Langmuir,1999,15.:743-746.
Lo Y S,Zhu Y J,Beebe Jr T P.Loading-rate dependence of individual ligand-receptor bond-rupture force studied by atomic force microscopy.Langmuir,2001,17:3741-3748.
Lovy W.A.,Robert M.L.,Raymond E.B.K,Christopher D.R.,Williams R.M.,Croteau R.Taxol Biosynthesis-Differential Transformations of Taxadien-5a-ol and Its Acetate Ester by Cytochrome P450 Hydroxylases from Taxus Suspension Cells.Archives of Biochemistry and Biophysics.2001,39:265-278.
Liu Q,Kasuga M,Sakuma Y,Abe H,Miura S,Yamaguchi-Shinozaki K,Shinozaki K.Two transcription factors,DREB1 and DREB2,with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis. Plant Cell .1998,10:1391-1406.
Ludwig SR, Habera LF, Dellaporta SL. Lc, a Member of the Maize R Gene Family Responsible for Tissue-Specific Anthocyanin Production, Encodes a Protein Similar to Transcriptional Activators and Contains the myc-Homology Region. Proceedings of the National Academy of Sciences of the United States of America. 1989, 86(18): 7092-7096.
Magnani E, Sjolander K, Hake S. From endonucleases to transcrip tion factors: evolution of the AP2 DNA binding domain in plants. Plant Cell, 2004, 16: 2265-2277.
Mahmoud SS ,Croteau R. Metabolic engineering of essential oil yield and composition in mint by altering expression of deoxyxylulose phosphate reductoisomerase and menthofuran synthase. Proc Natl Acad Sci USA, 2001 ,98: 8915-8920.
Matton DP, Prescott G, Bertrand C, Camirand A, Brisson N. Identification of cis-acting elements involved in the regulation of the pathogenesis-related gene Sth-2 in potato. Plant Mol.Biol., 1993; 22: 279-291.
Markus L. B., Raymond E. B., Croteau R. B. Metabolite profiling of peppermint oil gland secretory cells and application to herbicide target analysis. Plant Physiology. 2001,127:305-314.
Markus L. B., Rujan T., Martin W., Croteau R. Isoprenoid biosynthesis: The evolution of two ancient and distinct pathways across genomes. The Proceedings of the National Academy of Sciences of the United States of American. 2000, 97: 13172-13177.
Mahmoud SS, Croteau RB. Strategies for transgenic manipulation of monoterpene biosynthesis in plants. Trends Plant Sci., 2002 ,7: 366- 373.
Markus L.B. Crosstalk between cytosolic and plastidial pathways of isoprenoid biosynthesis in Arabidopsis thaliana. The Proceedings of the National Academy of Sciences of the United States of America, 2003,100: 6866-6871.
Mathews H, Clendennen S K, Caldwell C G, et al. Activation tagging in tomato identifies a transcriptional regulator of anthocyanin biosynthesis, modification, and transport [J]. Plant Cell,2003,15:1689-1703.
Meissner R C, Jin H L, Cominelli E. Function search in a large transcription factor gene family in Arabidopsis:assessing the potential of reverse genetics to identify insertional mutations in R2R3 MYB genes.Plant Cell,1999,11:1827-1840.
Memelink J,Verpoorte R,Kijne J W.ORCAnization of jasmonate-responsive gene expression in alkaloid metabolism.Trends Plant Sci.,2001,65:212-219.
Menke F L H,Champion A,Kijne J W,Memelink J.A novel jasmonate-and elicitor-responsive element in the periwinkle secondary metabolite biosynthetic gene STR interacts with a jasmonate-and elicitor-inducible AP2-domain transcription factor.ORCA2.EMBO,1999,18:4455-4463.
Miroslawa F,Kazimierz G,Katarzyna S B,Grazyna Z.Effect of picloram and methyl jasmonate on growth and taxane accumulation in callus of Taxus media var.hatfieldii.Plant Cell Tiss.Org.Cult.,1997,49:75-79.
Moon WJ,Yoo BS,Kim D et al.Elicitation kinetics of taxane production in suspension cultures of Taxus baccata Pendula.Biotechnology Techniques,1998,12(1):79-81.
Nakai K,Kanehisa M.A Knowledge base for predicting protein localization sites in Eukaryotic Cells.1992,14(4):897-911.
Narita JO,Gruissem W.Tomato hydroxymethylglutaryl-CoA reducetase is required early in fruit development but not during repening.Plant Cell,1989,1:181-190.
Nesi N,Debeaujon I,Jond C.The TT8 gene encodes a basic Helix-Loop-Helix domain protein required for expression of DFR and BAN genes in Arabidopsis siliques.Plant Cell,2000,(12):1863-1878.
Nesi N,Debeaujon I,Jond C,et al.The TRANSPARENT TESTA16 locus encodes the ARABIDOPSIS BSISTER MADS domain protein and is required for proper development and pigmentation of the seed coat[J].Plant Cell,2002,14(10):2463-2479.
Nishiyama C,Takahashi K,Ohtake Y.Analysis of transcription region of Eif-1 by using a yeast one-hybrid system.Biosci.Biotechnol.Biochem.,2002,66:1105-1107.
Paz-Ares,J,Ghosal,D,Wienand,U,Peterson,P A,and Saedler,H.The regulatory c7locus of Zea mays encodes a protein with homology to myb proto-oncogene products and with structural similarities to transcriptional activators.EMBO,1987,6:3553-3558.
Palaz(?)n J.,Cusid(?) R.M.,Bonfill M.,Morales C.,Pi(?)ol M.T.Inhibition of paclitaxel and baccatin Ⅲ accumulation by mevinolin and fosmidomycin in suspension cultures of Taxus baccata.Journal of Biotechnology,2003,101:157-163.
Pastuglia M,RuffioChable V,Delorme V,Gaude T,Dumas C,Cock JM.A functional S locus anther gene is not required for the self-incompatibility response in Brassica oleracea.Plant Cell,1997;9(11):2065-2076.
Petroni K,Cominelli E,Consonni G,et al.The tissue specific expression of the maize regulatory gene Hopi determines germination-dependent anthocyanin accumulation[J].Genetics,2000,155:323-336.
Qin F,Li J,Zhang G Y.Isolation and Structural Analysis of DRE-Binding Transcription Factor from Maize(Zea mays L.).Acta Botanica Sinica,2003,45(3):82.
Quattrocchio F,Wing J F,van der Woude K,et al.Analysis of bHLH and MYB-domain proteins:Species-specific regulatory differences are caused by divergent evolution of target anthocyanin genes[J].Plant J,1998,13:475-488.
Quattrocchio F,Wing J,van der Woude K,et al.Molecular analysis of the anthocyanin2 gene of petunia and its role in the evolution of flower color[J].Plant Cell,1999,11:1433-1444.
Rasmussen S,Dixon RA.Transgene2mediated and elicitor-induced perturbation of metabolic channeling at the entry point into the phenylpropanoid pathway.Plant Cell,1999,11:1537-1551.
Reymond P,Kunz B,Paul-Pletzer K,Grimm R,Eckerskom C,Farmer E E.Cloning of a cDNA encoding a plasma membrane-associated,uronide binding phosphoprotein with physical properties similar too viral movement proteins.Plant Cell,1996,8(12):2265-2276.
Riechmann J.L.,Heard J.,Martin G.,et al.Arabidopsis transcription factors:genome-wide comparative analysis among eukaryotes.Science,2000,290(24):2105-2110.
Robin L,Edward E.Farmer.The jasmonate pathway.Science,2002,296:1649-1650.
Roder K,Schweizer M.Running-buffer composition influences DNA-protein complexes detected by electrophoretic mobility-shift assay(EMSA).Biotechnol Appl Biochem,2001,33:209-214.
Sabah Hedhili,Nathalie Giglioli.Regulation of the terpene moiety biosynthesis of Catharanthus roseus terpene indole alkaloids,Phytochem Rev,2007,6:341-351.
Sagasser M,Lu G H,Hahlbrock K.A.thaliana TRANSPARENT TEATA1 is involved in seed coat development and defines the WIP subfamily of plant zine finger proteins.Genes Dev.,2002,16:138-149.
Sakamoto T,Ohmori T,Kageyama K,et al.The purple leaf(P1)locus of rice:the P1(w) allele has a complex organization and includes two genes encoding basic helix-loop-helix proteins involved in anthocyanin biosynthesis[J].Plant Cell Physiol,2001,42(9):982-991.
Sakuma Y,Liu Q,Dubouzet JG.DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs,transcription factors involved in dehydration-and cold-inducible gene expression.Biochemical and Biophysical Research Communications,2002,290(3):998-1009.
Sainz M.B.,Grotewold E.,Chandle V.L.Evidence for direct activation of an anthocyanin promoter by the maize C1 protein and comparison of DNA bind by related Myb domain proteins.Plant Cell,1997,9(3):611-625.
Saitou N,Nei M.The Neighbor-Joining Method-a new method for reconstructing phylogenetic trees.Molecular Biology and Evolution.1987,4(4):406-425.
Yoh Sakuma,Liu Q,Joseph G,et al.DNA-Binding Specificity of the ERF/AP2Domain of Arabidopsis DREBs,Transcription Factors Involved in Dehydration-and Cold-Inducible Gene Expression.Biochemical and Biophysical Research Communications,2002,290:998-1009
Sano H,Seo S,Koizumi N.Regulation by cytokinins of endogenous levels of jasmonic and salicylic acids in mechanically wounded tobacco plants.Plant cell physiol.,1996,37:762-769.
Schoendorf A,Rithner CD,Williams RM,Croteau R.Molecular cloning of a cytochrome P450 taxane 10_-hydroxylase cDNA from Taxus and functional expression in yeast.Proc.Natl.Acad.Sci.USA.2001,98:1501-1506.
Schwechheimer C,Bevan M.The regulation of transcription factor activity in plants.Trend in Plant Science,1998,3(10):278-283.
Schwesinger F,Ros R,Strunz T,et al.Unbinding forces of single antibody-antigen complexes correlate with their thermal.dissociation rates.Proc Natl Acad Sci USA,2000,97:9972-9977.
Sembdner G,Parthier B.The biochemistry and the physiological and molecular action of jasmonates.Ann.Rev.Plant Physiol.Plant Mol.Biol.,1993,44:569-589.
Sepaniak M,Datskos P,Lavrik N,Tipple C.Microcantilever transducers:a new approach in sensor technology.Analytical Chemistry,2002,November:568A-575A.
Shewmaker C K,Sheehy J A,Daley M,Colbum S,Ke D Y.Seed-specific overexpression of phytoene synthase:increase in carotenoids and other metabolic effects.Plant J.,1999,20:401-412.
S.H.Shin,M.C.Shih,W.H.Li.Transcription Factor Families Have Much Higher Expansion Rates in Plants than in Animals.Plant Physiology,2005,139(115):18-26.
Shah,J,Klessig D F.Identification of a salicylic acid-responsive element in the promoter of the tobacco pathogenesis-related 1,3-β-glucanase gene,PR-2d.Plant J.,1996,10(6):1089-1101.
ShinB.,ChoiQ,Yi H,et al.AtMyb21,a gene encodinga flower-specific tanscription factor,is regulated by COP1.The Plant Journal,2002,30(5):23-32.
Soave C.,Tardani L.,Fonzo N.,et al.Zein level in maize endosperm depends on a protein under control of the opaque-2 and opaque-6 loci.Ce11,1981,27(12):403-410.
Spelt C,Quattrocchio F,Mol J,et al.Anthocyaninl of petunia encodes a basic-helix loop helix protein that directly activates structural anthocyanin genes[J].Plant Cell,2000,12:1619-1631.
Stark D M,Timmerman K P,Barry G F,Preiss J & Kishore G M.Regulation of the amount of starch in plant tissue by ADP glucose pyrophosphorylase,Science,1992,258:287-292.
Tang Z,Zhao Y,Mei E Molecular cloning and characterization of a human gene involved in transcriptional regulation of hTERT.Biochem-Biophys-Res-Commun,2004,324(4):1324.
Thompson JD,Higgins DG,Gibson TJ.Clustal-W-Improving the sensitivity of progressinve multiple sequence alignment through sequence weighting,position-specific gap penalities and weight matrix choice.Nucleic Acids Res.,1994,22(22):4673-4680.
Tonelli C,Consonni G,Faccio D S,et al.Genetic and molecular analysis of Sn,a light-inducible,tissue specific regulatory gene in maize[J].Mol Gen Genet,1991,225:401-410.
van der Fits L.,Memelink J.ORCA3,a Jasmonate-Responsive Transcriptional Regulator of Plant Primary and Secondary Metabolism.Science,2000,289:.295-297.
Vincentz-Carbajosa J,Moose S P,Parsons R L.A maize zinc-finger protein binds the prolamin box in zein gene promoters and interacts with the basic leucine zipper transcriptional activator Opaque2.Proc.Natl.Acad.Sci.USA,1997,94:7685-7690.
Ramsay N A,Glover B J.MYB-bHLH-WD40 protein complex and the evolution of cdlular diversity.Trends Plant Sci.,2005,10(2):63-70.
Richter S,Lamppa GK.Determinants for removal and degradation of transit peptids of chloroplast precursor protein.J.Biol.Chem.,2002,277(46):43888-43894.
Riechmann J.L.,Heard J.,Martin G,et al.Arabidopsis transcription factors:genome-wide comparative analysis among eukaryotes.Seience,2000,290(24):2105-2110.
Rongmin Chen,Zhongfu Ni,Xiuling Nie,Yuxiang Qin,Guoqing Dong,Qixin Sun.Isolation and characterization of genes encoding Myb transcription factor in wheat(Triticum aestivem L.).Plant Science 169(2005) 1146-1154.
Ros R,Schwesinger F,Anselmetti D,Kubon M,Sehafer R,Pliickthun A,Tiefenauer L.Antigen binding,forces of individually addressed single-chain Fv antibody molecules.Proc.Natl.Acad.Sci.,1998,95:7402-7405.
Walker A R.Davison P A,Bolognesi-Winfield A C,et al.The TRANSPARENT TESTA(LA BRA I locus,which regulates trichome differentiation and anthocyanin biosynthesis in Araidopsis,encodes a WD40 repeat protein[J].Plant Cell,1999,11:1337-1350.
Walker K.,Croteau R.Taxol biosynthesis:Molecular cloning of a benzoyl-CoA:taxane 2a-O-benzoyltransferase cDNA from Taxus and functional expression in Escherichia coli.The Proceedings of the National Academy of Sciences of the United States of American.2000,97:99164-6340.
Walker K.,Fujisaki S.,Long R.,Croteau R.The final acylation step in Taxol biosynthesis:Cloning of the taxoid C13-side-chain N-benzoyltransferase from Taxus.The Proceedings of the National Academy of Sciences of the United States of American,2002,99:9166-9171.
Walker K.,Croteau R.Molecular cloning of a 10-deacetylbaccatin Ⅲ-10-O-acetyl transferase cDNA from Taxus and functional expression in Escherichia coli.The Proceedings of the National Academy of Sciences of the United States of America,2000,97(2),583-587.
WalkerK.,Croteau R.Taxol biosynthesis:Molecular cloning of a benzoyl-CoA:taxane 2α-O-benzoyltransferase cDNA from Taxus and functional expression in Escherichia coli.The Proceedings of the National Academy of Sciences of the United States of American,2000,97:99164-6340.
Walker K.,Fujisaki S.,Long R.,and Croteau R.Molecular cloning and heterologous expression of the C-13 phenylpropanoid side chain-CoA acyltransferase that functions in Taxol biosynthesis.The Proceedings of the National Academy of Sciences of the United States of American,2002,99,12715-12720.
Wang Y.D.,Yuan Y.J.,Wu J.C.Translocation of isopentenyl pyrophosphate for Taxol biosynthesis in suspension cultures of Taxus chinensis var.mairei.Plant Cell,Tissue and Organ Culture,2003,74,283-288.
Wani M.C.,Taylor H.L.,Wall M.E.,Coggon P.,McPhail A.T.Plant antitumor agents.The isolation and structure of taxol,a novel antileukemic and antitumor agent from Taxus brevifolia.Journal of American Chemical Society.1971,93,2325-2327.
Weisshaar B,Armstrong G A,Block A,et al.Light-inducible and constitutively expressed DNA-binding proteins recognizing a plant promoter element with functional relevance in light responsiveness[J].EMBO J,1991,10(7):1777-1786.
Wheeler A.L.,Long R.M.,Ketchum R.E.B.,Rithner C.D.,Williams R.M.,Croteau R.Taxol Biosynthesis:Differential Transformations of Taxadien-5α-ol and Its Acetate Ester by Cytochrome P450 Hydroxylases from Taxus Suspension Cells.Biochimica et Biophysica Acta.2001,390(2):265-278.
Wildung M.R.,Croteau R.A.cDNA cloing for taxadiene synthase that catalyzes the committed step of Taxol biosynthesis.J.Biol.Chem.1996,271:9201-9204.
Willner I.Nanoparticle-and nanorod-biomaterial hybrid system for sensor,circuitry and motor applications.Contributions to Science,2005,3(1):79-91.
Wu G,Ji H F,Hansen K,Thundat T,Datar R,Cote R,Hagan M F,Chakraborty A K,Majumdar A.Origin of nanomechanical cantilever motion generated from biomolecular interactions.Proc.Natl.Acad.Sci.,2001,98:1560-1564.
XueB.,CharestPJ.,Devantier Y,et al.Characterization of a MYBR2R3 gene from black spruce(Picea mariana)that shares functional conservation with maize C1.Mot.Genet.Genomics.,2003,270(35):78-86.
Xue G P.Characterization of the DNA-binding profile of barley HvCBF1 using an enzymatic method for rapid,quantitative and high-throughput analysis of the DNA-binding activity.Nucleic Acids Res.,2002,30:77.
Xue-Ping Li,Ai-Guo Tian,uang-Zuo Luo,Zhi-Zhong Gong,Jin-Song Zhang,Shou-Yi Chen.Soybean RE-binding transcription factors that are responsive to abiotic stresses.Theor Appl Genet,2005,110:1355-1362.
Ye X,Al-Babili S,Kloti A,Zhang J,Lucca P,Potrykus I.Engineering the provitamin A(beta-carotene) biosynthetic pathway into(carotenoid-free) rice endosperm.Science,2000,287:303-305.
Yazaki K,Takeda K,Tabata M.Effects of methyl jasmonate on shikonin and dihydroechinofuran production in Lithospermum cell cultures.Plant Cell Physiol.,1997,38:776-782.
Zamir L.O.,Nedea M.E.,Belair S.,Sauriol F.,Mamer O.,jacqmain E.,Jean F.I.,Garneau F.X.Biosynthetic building blocks of Taxus canadiensis taxanes.Tetrahedron Letters,1992,33,5235-5236.
Zhang L,Ding R X,Chai Y R.Engineering tropane biosynthetic pathway in Hyoscyamus niger hairy root cultures.Proc.Natl.Acad.Sci.USA,2004,101:6786-6791.
Zlatanova J,Lindsay S M,Leuba S H.Single molecule force spectroscopy in biology using the atomic force microscope.Prog Bioph Mole Biol,2000,74:37-61.
Zhou J M,Tang X Y,Martin GB.The Pto kinase coffering resistance to tomato bacterial speck disease interacts with proteins that bind a cis-element of pathogenesis2related genes[J].EMBO J,1997,16:3207-3218.
Zhulidov P A,Bogdanova E A,Shcheglov A S,Vagner L L.Simple cDNA normalization using kamchatka crab duplex-specific nuclease.Nucleic Acid.Res.,2004,32:37.
曹泽或,黄本开,王庆亚,宣伟,凌腾芳,张博,陈曦,聂理,沈文飚.血红素加氧酶催化产生的NO参与ABA诱导的蚕豆气孔关闭及其信号转导.科学通报,2007,52(15):1771-1778.
蔡昆争,董桃杏,徐涛.茉莉酸类物质(JAs)的生理特性及其在逆境胁迫中的抗性作用.生态环境,2006,15(2):397-404.
陈峰,余军平,方晓红,张贵友.鉴定水稻中一个新的专一结合GCC元件的AP2/EREBP族转录因子.生物化学与生物物理进展,2006,33(7):627-634.
陈晓亚,刘培.植物次生代谢的分子生物学及基因工程.生命科学,1996,8(2):8-11.
戴大临著.生物医学电镜样品制备方法.天津大学出版社.1993.
耿海波,安丽平,陈力,刘洁.抗癌药物喜树碱及其衍生物的研究进展.河北化工,2007,30(3):13-15.
杜丽娜,张存莉,朱玮,张高宏.植物次生代谢合成途径及生物学意义.西北林 学院学报,2005,20(3):150-155.
韩梅,赵博,安志刚,ThomasRausch,祖元刚.长春花萜类吲哚生物碱生物合成途径中重要酶(DXR、SLS、G10H、STR)基因的克隆与表达.植物研究,2001,27(5):564-568.
韩志萍,安利佳,侯和胜.AP2/EREBP转录因子的结构与功能.中国农学通报.2006,22(2):33-38.
何水林.参与植物次生代谢调控的转录因子及其在植物次生代谢遗传改良中的应用.热带亚热带植物学报,2004,12(4):374-380.
何水林,郑金贵,王晓峰,王燕华,许明,李斌莲,林明.植物次生代谢:功能、调控及其基因工程.应用与环境生物学报,2002,8(5):558-563.
李丽娟,赵奂,李艳军,刘祥林,何奕昆.植物中的DREB类转录因子.首都师范大学学报(自然科学版),2006,27(5):61-67.
李鹏,宁熙平,吴鸿.紫锥菊药用部位的结构及其与多酚类化合物积累的关系.中草药,2007,38(4):606-610
廖名湘,方福德.酵母单杂交体系-一种研究DNA-蛋白相互作用的有效方法.中国医学科学院学报,2000,22:388-391.
廖志华.紫杉醇前体生物合成的分子生物学和抗癌萜类吲哚生物碱的代谢工程,2005.博士学位论文.
刘强,张贵友,陈受益.植物转录因子的结构与调控作用.科学通报.2000,45(2):1465-147.
梅兴国,张舟宁,苏湘鄂,龚伟.水杨酸对红豆杉细胞的诱导作用.生物技术,2000,10(6):18-20.
秦峰,江雅新,马新勇,陈峰,方晓红,白春礼,李一勤.原子力显微镜(AFM)研究转录因子与DNA顺式作用元件间的特异性相互作用力.科学通报,2004,49(12):1157-1161.
秦秋琳 银杏中与木质素合成和苯丙氨酸代谢相关的转录调控因子的克隆与研究.2007.博士学位论文.
施中东,未作君,元英进.南方红豆杉细胞培养合成紫杉醇诱导子浓度的优化.天然产物研究与开发,2000,12(4):36-40.
苏湘鄂,梅兴国,龚伟,张舟宁.茉莉酸甲酯与真菌诱导物、水杨酸组合对红豆杉细胞中紫杉醇含量的影响.生物技术,2001,11(1):10-13.
谢永丽.一类植物中特有的转录因子—AP2/EREBP转录因子家族.青海师范大学学报(自然科学版),2006,(3):80-83.
杨致荣,毛雪,李润植.植物次生代谢基因工程研究进展.植物生理与分子生物 学学报.植物生理与分子生物学学报,2005,31(1):11-18.
王鹏.通过RNA介导的基因抑制对烟碱代谢调控的研究.2007.博士学位论文
吴大利等:干扰RNA(RNAi)作用机理及其在植物细胞工程中的应用进展,2006,26(9):84-90.
郑清平,余龙江,刘智,李默怡,向福,杨秦.红豆杉细胞非甲羟戊酸途径关键酶基因dxr的克隆与分析.生物工程学报,2004,20(4):548-553.
周忠强,梅兴国.水杨酸对细胞培养生产紫杉烷的影响.天然产物研究与开发,2002,14(2):11-13.
张剑峰,郭志刚.Effect of Methyl Jasmortic Acid on Baccatin Ⅲ Biosynthesis.清华大学学报(自然科学版)(英文版),2006,11(3):363-367.
张淼,赵畅,李法莲,邵群.具有AP2结构域的转录因子家族的研究进展.科技信息(学术研究),2007,(21):332-335.
张珏,吕加国;朱驹.抗肿瘤药物紫杉醇的化学研究进展.中国新药杂志,2006,15(3):178-181.
赵锋,胡鸢雷;祝建波;林忠平.厚叶悬蒴苣苔BcWRKY1基因启动子克隆及功能元件分析.生物技术通报,2007,(2):128-131.
朱佳.玉米籽粒中的转录因子研究.2007.硕士学位论文.
宗成文,章镇,房经贵,陶建敏,赵密珍.葡萄LEAFY基因启动子的克隆与序列分析.南京农业大学学报,2007,30(4):20-25.
金冬雁、黎孟枫等译,侯云德等校.分子克隆实验指南(第二版).科学出版社,1996年5月出版.
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