黑果枸杞色素微胶囊的制备
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摘要
本论文以新鲜黑果枸杞果实为原料,对黑果枸杞色素的提取方法、抗氧化活性及微胶囊制备工艺进行了研究,重点优化了微胶囊制备条件,可为黑果枸杞色素的开发利用提供科学依据。结果如下：
1、微波辅助黑果枸杞色素提取方法的优化。结果表明,以色素含量为考察指标,通过提取溶剂、辐射功率、提取时间、料液比和浸泡时间五个因素对黑果枸杞色素提取效果的影响进行了单因素试验和正交试验,黑果枸杞色素的优化提取条件为：提取溶剂为75%乙醇,辐射功率为70 w,提取时间为20 min,料液比为1：50,浸泡时间为20 h,在此条件下色素提取率为15.32%,总花色苷含量936.27 mg/100 g。该方法具有操作简单、提取时间短、提取率高等优点。
2、黑果枸杞色素的抗氧化活性。采用体外自氧化模拟方法测定黑果枸杞色素抗氧化活性,黑果枸杞色素对·OH,O2-和DPPH·都有较好的清除作用,通过D-半乳糖胺、四氯化碳、扑热息痛等药物制造肝损伤模型研究黑果枸杞色素的保肝活性,用化学方法测定血清中谷丙氨酸转移酶、超氧歧化酶,肝脏中肝糖含量及小鼠肝细胞的形态变化。结果表明,在试验条件下,黑果枸杞色素对三种药物导致的肝损伤有一定保护作用,黑果枸杞色素具有较强的体内外抗氧化活性。
3、枸杞色素微胶囊的制备。采用复凝聚凝胶法制备黑果枸杞色素微胶囊,其最佳制备工艺为：2%海藻酸钠,2%氯化钙,包埋时间为0.5h,壳聚糖浓度为1%,芯壁材之比为1：3。在最佳工艺条件下微胶囊的平均包埋率为70%,经微胶囊化的黑果枸杞色素稳定性明显提高。
This dissertation mainly studies on the extraction and antioxidant and microcapsules of pigment from Lycium ruthenicum Murr.Purposing on providing stable foundation for the further research and application of Lycium ruthenicum Murr pigment. The results showed as follows:
1. To study the microwave-assisted extraction methods of anthocyanin pigment from Lycium rethenicum Murr.The results showed that the optional conditions for the content of anthocyanin in Lycium ruthenicum Murr including content solvent, microwave power, microwave extraction time, ratio of solid to solution and immersed time duration were studied by single factor test and orthogonal test.The optimal microwave-assisted extraction conditions were as follows:content solvent was (75% HCl ethanol), microwave power was 70 W,extraction time was 20min,the ratio of solid to solution was 1:50, immersed time was 20 h.The optimized extraction technique yielded 15.32% of anthocyanin pigment,and the yield of total anthocyanins was up to 936.27 mg/100 g.The microwave-assisted extraction for anthocyanin pigment in Lycium rethenicum Murr is a time-and cost-saving and highly efficient method.
2. The antioxidant activity of pigment from Lycium rethenicum Murr. It has shown strong scavenging ability on O2-, OH, DPPH·.Studied on the effect of Pigment from Lycium ruthenicum Murr prevention and protection to the liver disease caused by the D-galactosamine, CCl4 and AAP others multi-drug. Methods:determined the antioxidant activity of Pigment from Lycium ruthenicum Murr through the self-oxidation simulation method in vitro, used injuried liver by D-galactosamine, CCl4 and AAP and others so as to research the hepatoprotective activity of it.Chemical methods were used to determinate the content of alanine acid transferase and superoxide dismutase in serum and hepatic glycogen of liver, observed the shape of liver cells. The results showed that Pigment from Lycium ruthenicum Murr have certain protective effect on liver injuryed by D-galactosamine, CCl4 and AAP others multi-drug, Pigment from Lycium ruthenicum Murr has high anti-oxidative activity in vivo and in vitro.
3. The optimal conditions for the preparation of pigment microcapsules:the alginate concentration was 2.0%, calcium chloride concentration was 2.0%, Embedding of time was 0.5 h, Chitosan concentration was 1.0%,the ratio of wall-material and core-material was 1:3 Under the optimum preparation technology, the embedded rate of Microcapsule was 70 percent.The stability of pigment can be improved significantly after microcapsuled.
1、微波辅助黑果枸杞色素提取方法的优化。结果表明,以色素含量为考察指标,通过提取溶剂、辐射功率、提取时间、料液比和浸泡时间五个因素对黑果枸杞色素提取效果的影响进行了单因素试验和正交试验,黑果枸杞色素的优化提取条件为：提取溶剂为75%乙醇,辐射功率为70 w,提取时间为20 min,料液比为1：50,浸泡时间为20 h,在此条件下色素提取率为15.32%,总花色苷含量936.27 mg/100 g。该方法具有操作简单、提取时间短、提取率高等优点。
2、黑果枸杞色素的抗氧化活性。采用体外自氧化模拟方法测定黑果枸杞色素抗氧化活性,黑果枸杞色素对·OH,O2-和DPPH·都有较好的清除作用,通过D-半乳糖胺、四氯化碳、扑热息痛等药物制造肝损伤模型研究黑果枸杞色素的保肝活性,用化学方法测定血清中谷丙氨酸转移酶、超氧歧化酶,肝脏中肝糖含量及小鼠肝细胞的形态变化。结果表明,在试验条件下,黑果枸杞色素对三种药物导致的肝损伤有一定保护作用,黑果枸杞色素具有较强的体内外抗氧化活性。
3、枸杞色素微胶囊的制备。采用复凝聚凝胶法制备黑果枸杞色素微胶囊,其最佳制备工艺为：2%海藻酸钠,2%氯化钙,包埋时间为0.5h,壳聚糖浓度为1%,芯壁材之比为1：3。在最佳工艺条件下微胶囊的平均包埋率为70%,经微胶囊化的黑果枸杞色素稳定性明显提高。
This dissertation mainly studies on the extraction and antioxidant and microcapsules of pigment from Lycium ruthenicum Murr.Purposing on providing stable foundation for the further research and application of Lycium ruthenicum Murr pigment. The results showed as follows:
1. To study the microwave-assisted extraction methods of anthocyanin pigment from Lycium rethenicum Murr.The results showed that the optional conditions for the content of anthocyanin in Lycium ruthenicum Murr including content solvent, microwave power, microwave extraction time, ratio of solid to solution and immersed time duration were studied by single factor test and orthogonal test.The optimal microwave-assisted extraction conditions were as follows:content solvent was (75% HCl ethanol), microwave power was 70 W,extraction time was 20min,the ratio of solid to solution was 1:50, immersed time was 20 h.The optimized extraction technique yielded 15.32% of anthocyanin pigment,and the yield of total anthocyanins was up to 936.27 mg/100 g.The microwave-assisted extraction for anthocyanin pigment in Lycium rethenicum Murr is a time-and cost-saving and highly efficient method.
2. The antioxidant activity of pigment from Lycium rethenicum Murr. It has shown strong scavenging ability on O2-, OH, DPPH·.Studied on the effect of Pigment from Lycium ruthenicum Murr prevention and protection to the liver disease caused by the D-galactosamine, CCl4 and AAP others multi-drug. Methods:determined the antioxidant activity of Pigment from Lycium ruthenicum Murr through the self-oxidation simulation method in vitro, used injuried liver by D-galactosamine, CCl4 and AAP and others so as to research the hepatoprotective activity of it.Chemical methods were used to determinate the content of alanine acid transferase and superoxide dismutase in serum and hepatic glycogen of liver, observed the shape of liver cells. The results showed that Pigment from Lycium ruthenicum Murr have certain protective effect on liver injuryed by D-galactosamine, CCl4 and AAP others multi-drug, Pigment from Lycium ruthenicum Murr has high anti-oxidative activity in vivo and in vitro.
3. The optimal conditions for the preparation of pigment microcapsules:the alginate concentration was 2.0%, calcium chloride concentration was 2.0%, Embedding of time was 0.5 h, Chitosan concentration was 1.0%,the ratio of wall-material and core-material was 1:3 Under the optimum preparation technology, the embedded rate of Microcapsule was 70 percent.The stability of pigment can be improved significantly after microcapsuled.
引文
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