短柄五加的化学成分及生物活性研究
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摘要
短柄五加(Acanthopanax brachypus)为五加科(Araliaceae)五加属(Acanthopanax)多年生落叶小灌木,在世界范围内的分布范围极其狭窄,主要分布于我国西北黄土高原上的陕西、甘肃、宁夏等地,是我国特有的民间药用植物。其药用部位主要为茎皮和根,具有益气健脾、养心安神、解郁和血等功效。据研究表明：其根茎的提取物除具有治疗神经衰弱、男子性功能障碍、继发性高血压、低血压、白细胞减少等疾病外,还有防癌抗癌功效；其皮的醇提物具有抗炎作用,还可抑制佐剂关节炎及Ⅰ、Ⅱ、Ⅲ型变态反应性炎症。
本文首先综述了目前国内外对五加属植物化学成分及生物活性的研究进展,在此基础上分析了五加属植物资源的开发现状及应用前景,并通过试管反应和薄层色谱法对短柄五加茎皮中的化学成分进行了系统预试验,为各类有效成分提取分离方案的设计提供依据。
采用水蒸汽蒸馏法分别提取了短柄五加花、叶、茎皮、根和果实中的挥发油,利用气相色谱-质谱联用技术对其化学成分进行了分离和结构鉴定,用面积归一化法测定了各成分的相对百分含量,通过体外抑菌试验研究了各挥发油的抑菌活性。结果表明,从短柄五加不同部位提取的挥发油中,化学成分的类型和相对含量不同,其中花、叶、茎皮和根部的挥发油以单萜烯、倍半萜烯及其氧化物、长链烃及衍生物等成分为主,为β-蒎烯／芳樟醇／吉玛烯-D型,而果实挥发油以脂肪酸(酯)为主。短柄五加挥发油中含有多种抑菌活性成分,对11种供试菌种均有不同程度的抑制作用,尤其是花和叶部位的挥发油对革兰氏菌的抑菌杀菌作用最为显著。
以乙醇为提取溶剂,采用超声波法提取了短柄五加茎皮中的非挥发性成分,并通过各种色谱(硅胶制备薄层色谱、硅胶柱色谱、大孔树脂和葡聚糖凝胶柱色谱)和重结晶等分离手段,从短柄五加茎皮中分离得到了52种单体化合物,通过对各种理化性质和波谱数据(包括紫外光谱、红外吸收光谱、核磁共振谱和质谱等)的分析,鉴定了46种化合物的结构,分别属于苯丙素、黄酮、酚酸、三萜及皂苷类等成分,它们分别为：(-)-海松-9(11),15-二烯-19-酸(1)、左旋贝壳杉-16-烯-19-酸(2)、β-谷甾醇(3)、6,7,10-三羟基-8-十八碳烯酸(4)、丁香树脂酚(5)、汉黄芩素(6)、藿香黄酮醇(7)、表儿茶素(8)、异嗪皮啶(9)、香草醛(10)、丹皮酚(11)、没食子酸甲酯(12)、丁二酸(13)、对-香豆酸(14)、咖啡酸(15)、水杨酸(16)、香草酸(17)、丁香酸(18)、胡萝卜苷(19)、扁枝衣二酸(20)、邻甲氧基苯酚-O-β-D-吡喃葡萄糖苷(21)、(E)-3-O-β-D-吡喃葡萄糖基茴香脑(22)、水杨苷(23)、天麻苷(24)、紫丁香苷(25)、地衣酚-O-β-D-吡喃葡萄糖苷(26)、白桦脂酸(27)、绿原酸(28)、山奈酚-7-O-β-D-吡喃葡萄糖苷(29)、奥卡宁-4-甲醚-3′-O-β-D-葡萄糖苷(30)、黄芩苷(31)、金丝桃苷(32)、丁子香酚-O-β-D-吡喃木糖基-(1→6)-β-D-吡喃葡萄糖苷(33)、(E)-白藜芦醇3-O-β-D-吡喃葡萄糖苷(34)、3α-(E)-芥子酰氧基泽兰醇18-O-β-D-吡喃葡萄糖苷(35)、丁香树脂酚-4,4′-二-O-β-D-双葡萄糖苷(36)、牡荆苷(37)、荭草苷(38)、3-O-甲基-(E)-白藜芦醇5-O-β-D-呋喃芹糖基-(1→6)-β-D-[2″-香草酰基]-吡喃葡萄糖苷(39)、3-O-甲基-(E)-白藜芦醇5-O-α-L-吡喃鼠李糖基-(1→6)-β-D-[2″-(E)-阿魏酰基]-吡喃葡萄糖苷(40)、3-O-甲基-(E)-白藜芦醇5-O-β-D-呋喃芹糖基-(1→6)-β-D-[2″-(E)-肉桂酰基]-吡喃葡萄糖苷(41)、3α,11α-二羟基羽扇豆-20(29)-烯-28-酸28-O-α-L-鼠李糖基-(1→4)-β-D-葡萄糖基-(1→6)-β-D-葡萄糖基酯(42)、3β,23,29-三羟基齐墩果-12-烯-28-酸28-O-α-L-鼠李糖基-(1→4)-β-D-葡萄糖基-(1→6)-β-D-葡萄糖基酯(43)、3-O-β-D-吡喃半乳糖基-(1→2)-[β-D-吡喃木糖基-(1→3)]-β-D-葡萄糖醛酸基-2β,3β,16α,23-四羟基齐墩果-12-烯-28-酸28-O-β-D-呋喃芹糖基-(1→3)-β-D-吡喃木糖基-(1→4)-a-L-吡喃鼠李糖基-(1→2)-a-L-阿拉伯糖基酯(44)、3β-O-β-D-葡萄糖基-(1→3)-α-L-鼠李糖基-(1→2)-α-L-阿拉伯糖基金合欢酸28-O-α-L-鼠李糖基-(1→4)-β-D-葡萄糖基-(1→6)-β-D-葡萄糖基酯(45)和3β-O-β-D-葡萄糖基-(1→2)-α-L-阿拉伯糖基齐墩果酸28-O-α-L-鼠李糖基-(1→4)-β-D-葡萄糖基-(1→6)-β-D-葡萄糖基酯(46)。其中35、39、40、41和44为未见文献报道的新化合物,化合物4、6-8、10-14、16、20-22、24、26、29-31、33、34、37和38系首次从该属植物中分离得到,而化合物1、2、5、9、15、17-19、23、27、28、32、42、43、45和46为首次从短柄五加中分离得到。
采用超声-微波辅助流动提取技术(UMAFE),分别对短柄五加叶中的总黄酮、总皂苷及茎皮中的多糖类成分进行提取分离。以各类成分的提取率为指标,通过正交试验,考察了各因素对提取率的影响,优选出了最佳提取工艺。研究结果显示,短柄五加中含有丰富的黄酮、皂苷和多糖类成分,其中叶中总黄酮和总皂苷的含量分别为22.95和9.67mg／g,茎皮中多糖的含量为56.37 mg／g。与其它提取方法相比较,用超声-微波辅助流动法来提取短柄五加中的总黄酮、皂苷和多糖类成分,具有提取时间短、提取率高、能耗低、有效成分不易破坏等特点。
Acanthopanax brachypus Harms is a small perennial deciduous shrub, which belongs to the genus Acanthopanax (Araliaceae family), and distributed in a narrow geographical area in the world, most in Shanxi, Gansu and Ningxia province on the loess plateau of the Northwest of China. It is a peculiar folk medicinal plant. As medicinal parts, the root and stem bark of this plant have the effect that replenishing qi to invigorate the spleen, raising a heart to calm the nerves, dispelling melancholy, as well as activating the blood and so on. Studies have shown that the rhizome extract have been used for the treatment of neurasthenic, male sexual dysfunction, secondary hypertension, hypotension and leucopenia disease, also have the effect of prevention cancer and anti-cancer. The alcohol extract of bark have anti-inflammatory action, and also inhibit adjuvant arthritis, as well as I, II and III type allergic inflammation.
This paper firstly reviewed domestic and international research progress on the chemical constituent and bioactivity of Acanthopanax genus. Based on this, the present situation and exploiting prospect of the resources of Acanthopanax were analyzed. With the methods of tube reaction and thin-layer chromatography, systemic preparative test was used to research the chemical constituent of the bark of Acanthopanax brachypus, to provide the foundation for the designs of extracting and separating scheme on the different active components.
The essential oils from the flower, leaves, bark, root and fruit of A. brachypus were individually extracted by hydrodistillation, and their chemical constituents were isolated and characterized by means of gas chromatography-mass spectrum. The relative percentages of the constituent were obtained by peak-area normalization. The in vitro antimicrobial activities of the essential oil were evaluated. The results indicated that the oils from the flower, leaves, bark and root consisted mainly of monoterpene and sesquiterpene hydrocarbons, oxygenated monoterpenes and sesquiterpenes, as well as long chain hydrocarbons and its derivatives and so on, belonged toβ-pinene/linalool/Germacrene-D chemotype, while the fruit oil consisted mainly of fatty acids (esters). The essential oils from the flower and leaves contain various antimicrobial activity constituents, and have the inhibitory effect on 11 tested microorganisms at different degree, especially for gram-bacteria.
By the ultrasonic extraction method, non-volatile component was extracted from the ethanol extract of the bark of A. brachypus,52 compounds were isolated by various chroma-tographic (Preparation Thin-Layer chromatography, Silica gel, Macroporous resin and Sephadex column chromatography) and recrystallized methods. On the basis of the analysis of physico-chemical properties and spectral data (UV, IR, NMR and MS), the structure of 46 compounds, which belonging to phenylpropanoid, flavonoid, phenolic acid, triterpene and triterpenoid saponin and so on, were identified as:(-)-pimara-9(11),15-dien-19-oic acid (1), (-)-kaur-16-en-19-oic acid (2),β-sitosterol (3),6,7,10-trihydroxy-8-octadecen oic acid (4), syringaresinol (5), wogonin (6), pachypodol (7), epicatechin (8), isofraxidin (9), vanillin (10), paeonol (11), methyl gallate (12), succinic acid (13), p-coumaric acid (14), caffeic acid (15), salicylic acid (16), vanillic acid (17), syringic acid (18), daucosterol (19), evernic acid (20), 2-methoxyphenyl-O-β-D-glucopyranoside (21), (E)-3-O-β-D-glucopyranosylanethole (22), salicin (23), gastrodin (24), syringin (25), orcinol-O-β-D-glucopyranoside (26), betulinic acid (27), chlorogenic acid (28), kaempferol-7-O-β-D-glucopyranoside (29), okanin 4-methyl ether-3'-O-β-D-glucopyranoside (30), baicalin (31), hyperoside (32), eugenol-O-β-D-xylopyrano syl-(1→6)-β-D-glucopyranoside (33), (E)-resveratrol 3-O-β-D-glucopyranoside (34),3a-(E)-sinapoyloxy-jhanol 18-O-β-D-glucopyranoside (35), (-)-syringaresinol-4,4'-di-O-β-D-glucop yranoside (36), vitexin (37), orientin (38),3-O-methyl-(E)-resveratrol 5-O-β-D-apiofuranosyl-(1→6)-β-D-[2"-vanilloyl]-glucopyranoside (39),3-O-methyl-(E)-resveratrol 5-O-a-L-rhamn opyranosyl-(1→6)-β-D-[2"-(E)-feruloyl]-glucopyranoside (40),3-O-methyl-(E)-resveratrol 5-O-β-D-apiofuranosyl-(1→6)-β-D-[2"-(E)-cinnamoyl]-glucopyranoside (41),3α,11α-dihydro xylup-20(29)-en-28-oic acid 28-O-α-L-rhamnopyranosyl-(1→4)-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl ester (42),3β,23,29-trihydroxy-olean-12-en-28-oic acid 28-O-a-L-rham nopyranosyl-(1→4)-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl ester (43),3-O-β-D-galactopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucuronopyranosyl-2β,3β,16α,23-tetrahydroxy olean-12-en-28-oic acid 28-O-β-D-apiofuranosyl-(1→3)-β-D-xylopyranosyl-(1→4)-a-L-rhamnopyranosyl-(1→2)-a-L-arabinopyranosyl ester (44),3β-O-β-D-glucopyrano syl-(1→3)-a-L-rhamnopyranosyl-(1→2)-a-L-arabinopyranosyl acacic acid 28-O-a-L-rhamno pyranosyl-(1→4)-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl ester (45),3β-O-β-D-gluco pyranosyl-(1→2)-a-L-arabinopyranosyl oleanolic acid 28-O-a-L-rhamnopyranosyl-(1→4)-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl ester (46). Among them,35,39,40,41 and 44 are firstly reported as new compounds, compounds 4,6-8,10-14,16,20-22,24,26,29-31,33, 34,37 and 38 were isolated from this genus for the first time, and compounds 1,2,5,9,15, 17-19,23,27,28,32,42,43,45 and 46 were obtained from this plant for the first time.
The ultrasonic-microwave assisted-flow extraction (UMAFE) method was used to extract total flavonoid, saponin and polysaccharide from the leaves and bark of A. brachypus, respectively. The various factors were investigated with the extraction efficiency by orthogonal test design. The optimum extracting processes were optimized. The result indicat- ed that A. brachypus contains rich flavonoid, saponin and polysaccharide. The contents of total flavonoid, saponin and polysaccharide were 22.95,9.67 and 56.37 mg/g in the leaves and bark of A. brachypus, respectively. Comparing with the other extraction methods, UMAFE is characteristic of short time, high efficiency, low energy consumption and stable structure.
本文首先综述了目前国内外对五加属植物化学成分及生物活性的研究进展,在此基础上分析了五加属植物资源的开发现状及应用前景,并通过试管反应和薄层色谱法对短柄五加茎皮中的化学成分进行了系统预试验,为各类有效成分提取分离方案的设计提供依据。
采用水蒸汽蒸馏法分别提取了短柄五加花、叶、茎皮、根和果实中的挥发油,利用气相色谱-质谱联用技术对其化学成分进行了分离和结构鉴定,用面积归一化法测定了各成分的相对百分含量,通过体外抑菌试验研究了各挥发油的抑菌活性。结果表明,从短柄五加不同部位提取的挥发油中,化学成分的类型和相对含量不同,其中花、叶、茎皮和根部的挥发油以单萜烯、倍半萜烯及其氧化物、长链烃及衍生物等成分为主,为β-蒎烯／芳樟醇／吉玛烯-D型,而果实挥发油以脂肪酸(酯)为主。短柄五加挥发油中含有多种抑菌活性成分,对11种供试菌种均有不同程度的抑制作用,尤其是花和叶部位的挥发油对革兰氏菌的抑菌杀菌作用最为显著。
以乙醇为提取溶剂,采用超声波法提取了短柄五加茎皮中的非挥发性成分,并通过各种色谱(硅胶制备薄层色谱、硅胶柱色谱、大孔树脂和葡聚糖凝胶柱色谱)和重结晶等分离手段,从短柄五加茎皮中分离得到了52种单体化合物,通过对各种理化性质和波谱数据(包括紫外光谱、红外吸收光谱、核磁共振谱和质谱等)的分析,鉴定了46种化合物的结构,分别属于苯丙素、黄酮、酚酸、三萜及皂苷类等成分,它们分别为：(-)-海松-9(11),15-二烯-19-酸(1)、左旋贝壳杉-16-烯-19-酸(2)、β-谷甾醇(3)、6,7,10-三羟基-8-十八碳烯酸(4)、丁香树脂酚(5)、汉黄芩素(6)、藿香黄酮醇(7)、表儿茶素(8)、异嗪皮啶(9)、香草醛(10)、丹皮酚(11)、没食子酸甲酯(12)、丁二酸(13)、对-香豆酸(14)、咖啡酸(15)、水杨酸(16)、香草酸(17)、丁香酸(18)、胡萝卜苷(19)、扁枝衣二酸(20)、邻甲氧基苯酚-O-β-D-吡喃葡萄糖苷(21)、(E)-3-O-β-D-吡喃葡萄糖基茴香脑(22)、水杨苷(23)、天麻苷(24)、紫丁香苷(25)、地衣酚-O-β-D-吡喃葡萄糖苷(26)、白桦脂酸(27)、绿原酸(28)、山奈酚-7-O-β-D-吡喃葡萄糖苷(29)、奥卡宁-4-甲醚-3′-O-β-D-葡萄糖苷(30)、黄芩苷(31)、金丝桃苷(32)、丁子香酚-O-β-D-吡喃木糖基-(1→6)-β-D-吡喃葡萄糖苷(33)、(E)-白藜芦醇3-O-β-D-吡喃葡萄糖苷(34)、3α-(E)-芥子酰氧基泽兰醇18-O-β-D-吡喃葡萄糖苷(35)、丁香树脂酚-4,4′-二-O-β-D-双葡萄糖苷(36)、牡荆苷(37)、荭草苷(38)、3-O-甲基-(E)-白藜芦醇5-O-β-D-呋喃芹糖基-(1→6)-β-D-[2″-香草酰基]-吡喃葡萄糖苷(39)、3-O-甲基-(E)-白藜芦醇5-O-α-L-吡喃鼠李糖基-(1→6)-β-D-[2″-(E)-阿魏酰基]-吡喃葡萄糖苷(40)、3-O-甲基-(E)-白藜芦醇5-O-β-D-呋喃芹糖基-(1→6)-β-D-[2″-(E)-肉桂酰基]-吡喃葡萄糖苷(41)、3α,11α-二羟基羽扇豆-20(29)-烯-28-酸28-O-α-L-鼠李糖基-(1→4)-β-D-葡萄糖基-(1→6)-β-D-葡萄糖基酯(42)、3β,23,29-三羟基齐墩果-12-烯-28-酸28-O-α-L-鼠李糖基-(1→4)-β-D-葡萄糖基-(1→6)-β-D-葡萄糖基酯(43)、3-O-β-D-吡喃半乳糖基-(1→2)-[β-D-吡喃木糖基-(1→3)]-β-D-葡萄糖醛酸基-2β,3β,16α,23-四羟基齐墩果-12-烯-28-酸28-O-β-D-呋喃芹糖基-(1→3)-β-D-吡喃木糖基-(1→4)-a-L-吡喃鼠李糖基-(1→2)-a-L-阿拉伯糖基酯(44)、3β-O-β-D-葡萄糖基-(1→3)-α-L-鼠李糖基-(1→2)-α-L-阿拉伯糖基金合欢酸28-O-α-L-鼠李糖基-(1→4)-β-D-葡萄糖基-(1→6)-β-D-葡萄糖基酯(45)和3β-O-β-D-葡萄糖基-(1→2)-α-L-阿拉伯糖基齐墩果酸28-O-α-L-鼠李糖基-(1→4)-β-D-葡萄糖基-(1→6)-β-D-葡萄糖基酯(46)。其中35、39、40、41和44为未见文献报道的新化合物,化合物4、6-8、10-14、16、20-22、24、26、29-31、33、34、37和38系首次从该属植物中分离得到,而化合物1、2、5、9、15、17-19、23、27、28、32、42、43、45和46为首次从短柄五加中分离得到。
采用超声-微波辅助流动提取技术(UMAFE),分别对短柄五加叶中的总黄酮、总皂苷及茎皮中的多糖类成分进行提取分离。以各类成分的提取率为指标,通过正交试验,考察了各因素对提取率的影响,优选出了最佳提取工艺。研究结果显示,短柄五加中含有丰富的黄酮、皂苷和多糖类成分,其中叶中总黄酮和总皂苷的含量分别为22.95和9.67mg／g,茎皮中多糖的含量为56.37 mg／g。与其它提取方法相比较,用超声-微波辅助流动法来提取短柄五加中的总黄酮、皂苷和多糖类成分,具有提取时间短、提取率高、能耗低、有效成分不易破坏等特点。
Acanthopanax brachypus Harms is a small perennial deciduous shrub, which belongs to the genus Acanthopanax (Araliaceae family), and distributed in a narrow geographical area in the world, most in Shanxi, Gansu and Ningxia province on the loess plateau of the Northwest of China. It is a peculiar folk medicinal plant. As medicinal parts, the root and stem bark of this plant have the effect that replenishing qi to invigorate the spleen, raising a heart to calm the nerves, dispelling melancholy, as well as activating the blood and so on. Studies have shown that the rhizome extract have been used for the treatment of neurasthenic, male sexual dysfunction, secondary hypertension, hypotension and leucopenia disease, also have the effect of prevention cancer and anti-cancer. The alcohol extract of bark have anti-inflammatory action, and also inhibit adjuvant arthritis, as well as I, II and III type allergic inflammation.
This paper firstly reviewed domestic and international research progress on the chemical constituent and bioactivity of Acanthopanax genus. Based on this, the present situation and exploiting prospect of the resources of Acanthopanax were analyzed. With the methods of tube reaction and thin-layer chromatography, systemic preparative test was used to research the chemical constituent of the bark of Acanthopanax brachypus, to provide the foundation for the designs of extracting and separating scheme on the different active components.
The essential oils from the flower, leaves, bark, root and fruit of A. brachypus were individually extracted by hydrodistillation, and their chemical constituents were isolated and characterized by means of gas chromatography-mass spectrum. The relative percentages of the constituent were obtained by peak-area normalization. The in vitro antimicrobial activities of the essential oil were evaluated. The results indicated that the oils from the flower, leaves, bark and root consisted mainly of monoterpene and sesquiterpene hydrocarbons, oxygenated monoterpenes and sesquiterpenes, as well as long chain hydrocarbons and its derivatives and so on, belonged toβ-pinene/linalool/Germacrene-D chemotype, while the fruit oil consisted mainly of fatty acids (esters). The essential oils from the flower and leaves contain various antimicrobial activity constituents, and have the inhibitory effect on 11 tested microorganisms at different degree, especially for gram-bacteria.
By the ultrasonic extraction method, non-volatile component was extracted from the ethanol extract of the bark of A. brachypus,52 compounds were isolated by various chroma-tographic (Preparation Thin-Layer chromatography, Silica gel, Macroporous resin and Sephadex column chromatography) and recrystallized methods. On the basis of the analysis of physico-chemical properties and spectral data (UV, IR, NMR and MS), the structure of 46 compounds, which belonging to phenylpropanoid, flavonoid, phenolic acid, triterpene and triterpenoid saponin and so on, were identified as:(-)-pimara-9(11),15-dien-19-oic acid (1), (-)-kaur-16-en-19-oic acid (2),β-sitosterol (3),6,7,10-trihydroxy-8-octadecen oic acid (4), syringaresinol (5), wogonin (6), pachypodol (7), epicatechin (8), isofraxidin (9), vanillin (10), paeonol (11), methyl gallate (12), succinic acid (13), p-coumaric acid (14), caffeic acid (15), salicylic acid (16), vanillic acid (17), syringic acid (18), daucosterol (19), evernic acid (20), 2-methoxyphenyl-O-β-D-glucopyranoside (21), (E)-3-O-β-D-glucopyranosylanethole (22), salicin (23), gastrodin (24), syringin (25), orcinol-O-β-D-glucopyranoside (26), betulinic acid (27), chlorogenic acid (28), kaempferol-7-O-β-D-glucopyranoside (29), okanin 4-methyl ether-3'-O-β-D-glucopyranoside (30), baicalin (31), hyperoside (32), eugenol-O-β-D-xylopyrano syl-(1→6)-β-D-glucopyranoside (33), (E)-resveratrol 3-O-β-D-glucopyranoside (34),3a-(E)-sinapoyloxy-jhanol 18-O-β-D-glucopyranoside (35), (-)-syringaresinol-4,4'-di-O-β-D-glucop yranoside (36), vitexin (37), orientin (38),3-O-methyl-(E)-resveratrol 5-O-β-D-apiofuranosyl-(1→6)-β-D-[2"-vanilloyl]-glucopyranoside (39),3-O-methyl-(E)-resveratrol 5-O-a-L-rhamn opyranosyl-(1→6)-β-D-[2"-(E)-feruloyl]-glucopyranoside (40),3-O-methyl-(E)-resveratrol 5-O-β-D-apiofuranosyl-(1→6)-β-D-[2"-(E)-cinnamoyl]-glucopyranoside (41),3α,11α-dihydro xylup-20(29)-en-28-oic acid 28-O-α-L-rhamnopyranosyl-(1→4)-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl ester (42),3β,23,29-trihydroxy-olean-12-en-28-oic acid 28-O-a-L-rham nopyranosyl-(1→4)-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl ester (43),3-O-β-D-galactopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucuronopyranosyl-2β,3β,16α,23-tetrahydroxy olean-12-en-28-oic acid 28-O-β-D-apiofuranosyl-(1→3)-β-D-xylopyranosyl-(1→4)-a-L-rhamnopyranosyl-(1→2)-a-L-arabinopyranosyl ester (44),3β-O-β-D-glucopyrano syl-(1→3)-a-L-rhamnopyranosyl-(1→2)-a-L-arabinopyranosyl acacic acid 28-O-a-L-rhamno pyranosyl-(1→4)-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl ester (45),3β-O-β-D-gluco pyranosyl-(1→2)-a-L-arabinopyranosyl oleanolic acid 28-O-a-L-rhamnopyranosyl-(1→4)-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl ester (46). Among them,35,39,40,41 and 44 are firstly reported as new compounds, compounds 4,6-8,10-14,16,20-22,24,26,29-31,33, 34,37 and 38 were isolated from this genus for the first time, and compounds 1,2,5,9,15, 17-19,23,27,28,32,42,43,45 and 46 were obtained from this plant for the first time.
The ultrasonic-microwave assisted-flow extraction (UMAFE) method was used to extract total flavonoid, saponin and polysaccharide from the leaves and bark of A. brachypus, respectively. The various factors were investigated with the extraction efficiency by orthogonal test design. The optimum extracting processes were optimized. The result indicat- ed that A. brachypus contains rich flavonoid, saponin and polysaccharide. The contents of total flavonoid, saponin and polysaccharide were 22.95,9.67 and 56.37 mg/g in the leaves and bark of A. brachypus, respectively. Comparing with the other extraction methods, UMAFE is characteristic of short time, high efficiency, low energy consumption and stable structure.
引文
[1]中国科学院中国植物志编辑委员会.中国植物志[M].北京：科学出版社,1978,54：86-116
[2]郑万均.中国树木志[M].北京：中国林业出版社,1985,2：1777-1780
[3]刘立品.子午岭木本植物志[M].兰州：兰州大学出版社,1998：290-291
[4]Yook C. S. Medicinal Herbs of Acanthopanax in Asia [M]. Seoul:Kyung-Won Media,2001:132-145
[5]Kim C. H., Sun B. Y., New Taxa and Combinations in Eleutherococcus (Araliacaea) from Eastern Asia [J]. Novan,2000,10 (3):209-214
[6]倪娜,刘向前.五加科五加属植物的研究进展[J].中草药,2006,37(12)：1895-1900
[7]赵余庆,袁昌鲁,杨志强.红毛五加挥发性成分的研究[J].中药材,1992,15(4)：28-31
[8]倪娜,赵君.GC-MS法分析红毛五加皮中挥发油的化学成分[J].化学,2007,2(11)：852-855
[9]张莅峡,刘泓,董建平.毛梗红毛五加皮挥发油化学成分的研究[J].中国药学杂志,1994,29(2)：83-86
[10]张莅峡,刘泓.红毛五加茎皮、叶、果实挥发油的GC-MS比较分析[J].中国中医基础医学杂志,2001,7(5)：24-27
[11]于万滢,张华,黄威东.多种气相色谱联用技术分析陕西刺五加茎挥发油的化学成分[J].色谱,2005,23(2)：196-201
[12]Yu W. Y., Zhang H., Huang W. D., et al. Analysis of the Volatile Oil from the Stem of Acanthopanax Senticosus (Rupr. et Maxim.) Harms with Several Hyphenated Methods of Chromatography [J]. Frontiers of Chemistry in China,2006,1 (2):193-198
[13]邢有权,孙志忠,韩晓玲,等.黑龙江刺五加根和茎挥发油成分的气相色谱-质谱分析[J].黑龙江大学学报(自然科学版),1992,9(4)：92-96
[14]薛兴亚,于万滢,陈吉平,等.气相色谱保留参数用于刺五加果挥发油中萜烯及萜烯氧化物的分类[J].色谱,2005,23(4)：422-425
[15]何方奕,李铁纯,李发美.气相色谱-质谱法分析无梗五加茎的挥发油成分[J].质谱学报,2004,25(2)：103-106
[16]刘向前,张承烨,印文教,等.细柱五加的挥发油成分分析[J].中草药,2001,32(12)：1074-1075
[17]Muselli A., Hoi T. M., Cu L. D., et al. Composition of the Essential Oil of Acanthopanax trifoliatus (L.) Merr. (Araliacaea) from Vietnam [J]. Flavour and Fragrance Journal,1999,14 (1):41-44
[18]Kiem P. V., Cai X. F., Minh C. V., et al. Lupane-Triterpene Carboxylic Acids from the Leaves of Acanthopanax trifoliatus [J]. Chemical& Pharmaceutical Bulletin,2003,51 (12):1432-1435
[19]刘向前,张承烨,陆昌洙.细柱五加叶中羽扇豆烷型三萜成分[J].兰州大学学报(自然科学版),2006,42(4)：86-91
[20]Yook C. S., Liu X. Q., Chang S. Y., et al. Lupane-Triterpene Glycosides from the Leaves of Acantho-panax gracilistylus [J]. Chemical& Pharmaceutical Bulletin,2002,50 (10):1383-1385
[21]Liu X. Q., Chang S. Y., Park S. Y., et al. Studies on the Constituents of the Stem Barks of Acanthopan ax gracilistylus W. W. Smith [J]. Natural product Science,2002a,50 (8):23-25
[22]Yook C. S., Kim I. H., Hahn D. R., et al. A Lupane-Triterpene Glycoside from Leaves of Two Acantho panax [J]. Phytochemistry,1998,49 (3):839-843
[23]Yook C. S., Chang S. Y., Lai J. H., et al. Lupane-Glycoside of Acanthopanax trifoliatus forma tristig matis Leaves [J]. Archives of Pharmacal Research,1999,22 (6):629-632
[24]Liu X. Q., Chang S. Y., Park S. Y. et al. A New Lupane-Triterpene Glycoside from the Leaves of Acanthopanax gracilistylus [J]. Archives of Pharmacal Research,2002,25 (6):831-836
[25]Chang S. Y., Yook C. S., Nohara T. Two New Lupane-Triterpene Glycosides from Leaves of Acantho panax koreanum [J]. Chemical& Pharmaceutical Bulletin,1998,46 (1):163-165
[26]Cai X. F., Lee I. S., Shen G. H., et al. Triterpenoids from Acanthopanax koreanum Root and Their Inhi bitory Activities on NFAT Transcription [J]. Archives of Pharmacal Research,2004,27 (8):825-828
[27]Chang S. Y., Yook C. S., Nohara T. Lupane-Triterpene Glycosides from Leaves of Acanthopanax koreanum [J]. Phytochemistry,1999,50 (8):1369-1374
[28]Choi H. S., Kim H. J., Nam S. G., et al. Lupane Glycosides from the Leaves of Acanthopanax korean um [J]. Chemical& Pharmaceutical Bulletin,2008,56 (11):1613-1616
[29]Ty Ph. D., Lischewski M., Phiet H. V., et al. Two Triterpenoid Carboxylic Acids from Acanthopanax trifoliatus [J]. Phytochemistry,1984,23 (12):2889-2891
[30]Ty P. D., Lischewski M., Phiet H. V., et al.3a,1 la-Dihydroxy-23-oxo-Lup-20(29)-en-28-oic Acid from Acanthopanax trifoliatus [J]. Phytochemistry,1985,24 (4):867-869
[31]Oh O. J., Chang S. Y., Yook C. S., et al. Two 3,4-seco-Lupane Triterpenes from Leaves of Acanthopan ax divaricatus var. albeofructus [J]. Chemical& Pharmaceutical Bulletin,2000,48 (6):879-881
[32]Won J. H., Park S. Y., Nam S. G., et al. Inhibition of Lipopolysaccharide-Induced Expression of Inducible Nitric Oxide and Cyclooxygenase-2 by Chiisanoside via Suppression of Nuclear Factor-KB Activation in RAW 264.7 Macrophage Cells [J]. Biological& Pharmaceutical Bulletin,2005,28 (10): 1919-1924
[33]Park S. Y., Chang S. Y., Yook C. S., et al. New 3,4-seco-Lupane-Type Triterpene Glycosides from Acanthopanax senticosus forma inermis [J]. Journal of Natural Products,2000,63 (12):1630-1633
[34]Shirasuna K., Miyakoshi M., Mimoto S., et al. Lupane Triterpenoid Glycosyl Esters from Leaves of Acanthopanax divaricatus [J]. Phytochemistry,1997,45 (3):579-584
[35]Hahn D. R., Kasai R., Kim J. H., et al. A New Glycosyl Ester of a 3,4-seco-Triterpene from Korean Medicinal Plant, Acanthopanax chiisanensis (Araliaceae) [J]. Chemical& Pharmaceutical Bulletin, 1984,32 (3):1244-1247
[36]Kasai R., Matsumoto K., Taniyasu S., et al.3,4-seco-Lupane-Type Triterpene Glycosyl Esters from a Korean Medicinal Plant, Acanthopanax chiisanensis (Araliaceae) [J]. Chemical& Pharmaceutical Bulletin,1986,34 (8):3284-3289
[37]安琪,杨春娟,宋洋,等.无梗五加果化学成分的研究[J].天然产物研究与开发,2008,20(5)：765-769
[38]王广树,陈燕萍,徐景达,等.无梗五加叶化学成分的研究[J].中国药学杂志,1997,32(1)：11-13
[39]Yoshizumi K., Hirano K., Ando H., et al. Lupane-Type Saponins from Leaves of Acanthopanax sessiliflorus and Their Inhibitory Activity on Pancreatic Lipase [J]. Jouranal of Agricultural and Food Chemistry,2006,54 (2):335-341
[40]Lischewski M., Ty P. D., Kutschabsky L., et al. Two 24-nor-Triterpenoid Carboxylic Acids from Acanthopanax trifoliatus [J]. Phytochemistry,1985,24 (10):2355-2357
[41]Park S. Y., Yook C. S., Nohara T. A Novel 3,4-seco-Migrated-Lupane Glycoside with a Seven-Memb ered B-ring from Acanthopanax divaricatus var. sachunensis [J]. Tetrahedron Letters,2001,42 (15): 2825-2828
[42]赵余庆,吴立军,李铣.刺五加活性成分Liriodendrin的结构研究[J].中草药,1990,21(3)：44-45
[43]Wenhong J., Wei L., Likun H., et al. Biologically Active Triterpenoid Saponins from Acanthopanax senticosus [J]. Journal of Natural Products,2006,69 (11):1577-1581
[44]Park S. Y, Choi H. S., Yook C. S., et al. A New Lupane Glycoside from the Leaves of Acanthopanax koreanum [J]. Chemical& Pharmaceutical Bulletin,2005,53 (1):97-99
[45]Kiem P. V., Minh C. V., Cai X. F., et al. A New 24-nor-Lupane-Glycoside of Acanthopanax trifoliatus [J]. Archives of Pharmacal Research,2003,26 (9):706-708
[46]Yang C. J., An Q., Xiong Z. L., et al. Triterpenes from Acanthopanax sessiliflorus Fruits and Their Antiplatelet Aggregation Activities [J]. Planta Medica,2009,75 (6):656-659
[47]Park S. Y., Chang S. Y., Oh O. J., et al. nor-Oleanene Type Triterpene Glycosides from the Leaves of Acanthopanax japonicus [J]. Phytochemistry,2002,59 (4):379-384
[48]Shao C. J., Kasai R., Xu J. D., et al. Saponins from Leaves of Acanthopanax senticosus Harms., Ciwujia. II.:Structures of Ciwujianosides A1, A2, A3, A4 and D3 [J]. Chemical& Pharmaceutical Bulletin,1989,37 (1):42-45
[49]Shao C. J., Kasai R., Xu J. D., et al. Saponins from Leaves of Acanthopanax senticosus Harms, Ciwujia:Structures of Ciwujianosides B, C1, C2, C3, C4, D1, D2 and E [J]. Chemical& Pharmaceutical Bulletin,1988,36 (2):601-608
[50]Miyakoshi M., Shirasuna K., Hirai Y, et al. Triterpenoid Saponins of Acanthopanax nipponicus Leaves [J]. Journal of Natural Products,1999,62 (3):445-448
[51]史良久.刺五加化学成分的研究[J].药学通报,1981,16(3)：53-56
[52]Miyakoshi M., Ida Y, Isoda S., et al.3-epi-Oleanene-Type Triterpene Glycosyl Esters from Leaves of Acanthopanax spinosus [J]. Phytochemistry,1993,33 (4):891-895
[53]Miyakoshi M., Ida Y, Isoda S., et al.3a-Hydroxy-Oleanane-Type Triterpene Glycosyl Esters from Leaves of Acanthopanax spinosus [J]. Phytochemistry,1993,34 (6):1599-1602
[54]Miyakoshi M., Isoda S., Sato H., et al.3a-Hydroxy-Oleanene Type Triterpene Glycosyl Esters from Leaves of Acanthopanax spinosus [J]. Phytochemistry,1997,46 (7):1255-1259
[55]张杰,李文,高慧媛,等.刺五加种子的化学成分[J].沈阳药科大学学报,2005,22(3)：183-186
[56]Kohda H., Tanaka S., Yamaoka Y. Saponins from Leaves of Acanthopanax hypoleucus Makino [J]. Chemical& Pharmaceutical Bulletin,1990,38 (12):3380-3383
[57]Li F., Li W., Fu H. W., et al. Pancreatic Lipase-Inhibiting Ttriterpenoid Ssaponins from Fruits of Acanthopanax senticosus [J]. Chemical& Pharmaceutical Bulletin,2007,55 (7):1087-1089
[58]程东亮,邵宇,杨立.红毛五加叶的三萜皂甙[J].植物学报,1994,36(1)：75-79
[59]孔德云,罗思弃.红毛五加化学成分研究Ⅰ[J].中国医药工业杂志,1990,21(5)：202-205
[60]Sawada H., Miyakoshi M., Isoda S., et al. Saponins from Leaves of Acanthopanax sieboldianus [J]. Phytochemistry,1993,34(4):1117-1121
[61]孔德云,金惠芳,罗思弃.红毛五加化学成分研究Ⅱ[J].中国医药工业杂志,1992,23(5)：215-217
[62]Park S. Y., Yook C. S., Nohara T. New Oleanene Glycosides from the Leaves of Acanthopanax japonicus [J]. Chemical& Pharmaceutical Bulletin,2005,53 (9):1147-1151
[63]刘向前,金寅钐,陆昌洙,等.异柱五加叶中齐墩果烷型皂苷的研究[J].天然产物研究与开发,2008,20(1)：66-69
[64]Nishibe S., Kinoshita H., Takeda H., et al. Phenolic Compounds from Stem Bark of Acanthopanax senticosus and Their Pharmacological Effect in Chronic Swimming Stressed Rats [J]. Chemical& Pharmaceutical Bulletin,1990,38 (6):1763-1765
[65]卫平,于高麦.短柄五加化学成分研究[J].中草药,1989,20(4)：8-10
[66]孙晓云.蜀五加化学成分的研究[J].天然产物研究与开发,2001,13(2)：34-68
[67]Elyakova L. A., Elyakov G. B. Glycosides from Roots of Acanthopanax sessiliflorum [J]. Russian Chemical Bulletin,1965,14 (3):537-539
[68]Kim J. H., Hahn D. R. Studies on the Chemical Constituents of Acanthopanax chiisanensis Nakai Roots [J]. Archives of Pharmacal Research,1981,4 (1):59-62
[69]Cai X. F., Lee I. S., Dat N. T., et al. Inhibitory Lignans against NEAT Transcription Factor from Acanthopanax koreanum [J]. Archives of Pharmacal Research,2004,27 (7):738-741
[70]赵余庆,杨松松,柳江华,等.刺五加化学成分的研究[J].中国中药杂志,1993,18(7)：428-429
[71]郭丽娜,吴立军,郭祥菊,等.无梗五加茎叶化学成分的研究[J].中草药,2006,37(6)：833-834
[72]吴立军,郑健,姜宝虹,等.刺五加茎叶化学成分[J].药学学报,1999,34(4)：294-296
[73]向仁德,徐任生.南五加皮化学成分的研究[J].植物学报,1983,25(4)：356-362
[74]刘向前,陆昌洙,张承烨.细柱五加化学成分的研究[J].中草药,2004,35(3)：250-252
[75]Sanghyun L., Jun J., Kuk H. S., et al. Sessiline, A New Nitrogenous Compound from the Fruits of Acanthopanax sessiliflorus [J]. Planta Medica,2002,68 (10):939-941
[76]Ban H. S., Lee S. H., Kim Y. P., et al. Inhibition of Prostaglandin E2 Production by Taiwanin C Isolatedfrom the Root of Acanthopanax chiisanensis and the Mechanism of Action [J]. Biochemical Pharmacology,2002,64 (9):1345-1354
[77]Yoo H. H., Lee S. H., Jin C. B., et al. Mechanism-Based Inactivation of Cytochrome P4503A4 by Methylenedioxyphenyl Lignans from Acanthopanax chiisanensis [J]. Planta Medica,2008,74 (8):
808-813
[78]Kim Y. H., Chung B. S., Ko Y. S., et al. Studies on the Chemical Constituents of Acanthopanax koreanum (Ⅱ) [J]. Archives of Pharmacal Research,1988,11 (2):159-162
[79]Elyakova L. A., Dzizenko A. K., Sova V. V., et al. (-)-Sesamin and (-)-Sainine from Acanthopanax sessiliflorus and Their NMR Spectra [J]. Chemistry of Natural Compounds,1966,2 (3):117-119
[80]吴立军,阮丽军,郑健.刺五加茎叶化学成分研究[J].药学学报,1999,34(11)：839-841
[81]苑艳光,王录全,吴立军,等.刺五加茎的化学成分[J].沈阳药科大学学报,2002,19(5)：325-327
[82]Zhou X. G., Zheng C. Y., Huang J. S., et al. Identification of Herb Acanthopanax senticosus (Rupr. Et Maxim.) Harms by Capillary Electrophoresis with Electrochemical Detection [J]. Analytical Sciences, 2007,23 (6):705-711
[83]韩莹,李遇伯,崔兰冲.HPLC法同时测定无梗五加果中东莨菪内酯和异嗪皮啶的含量[J].沈阳药科大学学报,2006,23(9)：73-576
[84]Won C. K. Study on the Constituents of Seeds of Acanthopanax sessiliflorum Seemann Ⅰ[J]. Saengyak Hakhoechi,1987,18 (3):184-187
[85]赵余庆,柳江华,赵光燃.刺五加中脂肪酸类和酯类成分的分离与鉴定[J].中医药学报,1989,15(3)：55-56
[86]Yook C. S., Oho L. P., et al. Study on the Constituents in the Root Barks of Acanthopanax sessiliflor us Seemann Ⅱ [J]. Saegyak Hakhoechi,1997,28 (1):31-34
[87]Asilbekova D. T. Gusakova S. D., Glushenkova A. I. Lipids of the Fruits of Acanthopanax sessilifl orus [J]. Chemistry of Natural Compounds,1985,21 (6):719-724
[88]Asilbekova D. T., Gusakova S. D., Glushenkova A. I. New Hydroxy Fatty Acids of Acanthopanax sessiliflorus [J], Chemistry of Natural Compounds,1991,27 (5):542-544
[89]Kiem P. V., Cai X. F., Minh C. V., et al. Kaurane-Type Diterpene Glycoside from the Stem Bark of Acanthopanax trifoliatus [J]. Planta Medica,2004,70 (3):282-284
[90]Kim Y. H., Kim H. S., Lee S. W., et al. Kaurane Derivatives from Acanthopanax koreanum [J]. Phytochemistry,1995,39 (2):449-451
[91]Cai X. F., Shen G. H., Dat N. T., et al. Inhibitory Effect of Kaurane-Type Diterpenoids from Acantho panax koreanum on TNF-a Secretion from Trypsin-Stimulated HMC-1 Cells [J]. Archives of Pharma-cal Research,2003,26 (9):731-734
[92]Na M. K., Oh W. K., Kim Y. H., et al. Inhibition of Protein Tyrosine Phosphatase 1B by Diterpenoids Isolated from Acanthopanax koreanum [J]. Bioorganic& Medicinal Chemistry Letters,2006,16 (11): 3061-3064
[93]Cai X. F., Shen G. H., Dat N. T., et al. Inhibitory Effect of TNF-α and IL-8 Secretion by Pimarane-Type Diterpenoids from Acanthopanax koreanum [J]. Chemical& Pharmaceutical Bulletin,2003,51 (5):605-607
[94]Kim Y. H., Chung B. S., Sankawa U. Pimaradiene Diterpenes from Acanthopanax koreanum [J]. Journal of Natural Products,1988,51 (6):1080-1083
[95]Phuong N. T., Lee K. A., Jeong S. J., et al. Capillary Electrophoretic Method for the Determination of Diterpenoid Isomers in Acanthopanax Species [J]. Journal of Pharmaceutical and Biomedical Analysis, 2006,40 (1):56-61
[96]Miyakoshi M., Tajima Y., Isoda S., et al. Constituents from the Leaves of Acanthopanax tricodon [J]. Nature Medicine,1997,51 (5):494-497
[97]陈貌连,宋凤瑞,郭明全,等.刺五加叶中黄酮类化合物的结构鉴定[J].高等学校化学学报,2002,23(5)：805-808
[98]Zhao W. M., Qin G. W., Xu R. S., et al. Constituents from the Roots of Acanthopanax setchuenensis [J]. Fitoterapia,1999,70 (5):529-531
[99]Feng S. L., Hu F. D., Zhao J. X., et al. Determination of Eleutheroside E and Eleutheroside B in Rat Plasma and Tissue by High-Performance Liquid Chromatography using Solid-Phase Extraction and Photodiode Array Detection [J]. European Journal of Pharmaceutics and Biopharmaceutics,2006,62 (3):315-320
[100]Jung H. J., Park H. J., Kim R. G., et al. In vivo Anti-Inflammatory and Antinociceptive Effects of Liriodendrin Isolated from the Stem Bark of Acanthopanax senticosus [J]. Planta Medica,2003,69 (7):610-616
[101]王子灿,乔善义,马安德,等.高效液相色谱-质谱联用技术分析刺五加抗疲劳化学成分[J].第一军医大学学报,2003,23(4)：355-357
[102]Fang J. N., Proksch A., Wagner H. Immunologically Active Polysaccharides of Acanthopanax senticosus [J]. Phytochemistry,1985,24 (11):2619-2622
[103]欧惠英,李廷昊,东美麟,等.吴茱萸五加化学成分的研究[J].中草药,1994,25(12)：625-625
[104]孔令义,邵春杰,徐景达.无梗五加根化学成分的研究[J].中草药,1988,19(11)：482-486
[105]斯建勇,陈迪华,常琪,等.倒卵叶五加根茎的化学成分[J].植物学报,1993,35(6)：483-485
[106]Fang J. N. Immunologically Active Polysaccharides of Acanthopanax senticosus [J]. Phytochemistry, 1995,24(11):2619-2622
[107]刘起华,朱札,李文兰.刺五加主要活性成分化学与药理研究[J].时珍国医国药,1999,10(5)：305-308
[108]李筱玲,邓寒霜.刺五加茎叶化学成分分析[J].商洛师范专科学校学报,2006,20(1)：103-105
[109]张丽萍,陈丽颖,庄会科,等.刺五加果化学成分的分析[J].东北师范大学学报(自然科学版),1992,24(1)：120-128
[110]金同顺,欧惠英.3种五加中微量元素和氨基酸含量分析[J].南京师范大学学报(自然科学版),1995,18(2)：45-49
[111]杨坡,孙宝俊,刘娥,等.短梗五加食药用价值及开发利用[J].中国野生植物资源,2006,25(2)：43-44
[112]郭丽娜.无梗五加化学成分与药理活性的研究进展[J].中国药业,2002,11(12)：73-74
[113]杨勤福,窦忠健.无梗五加的研究[J].中医研究,1999,12(6)：14-15
[114]李兴玉,魏虎来,张吉旺,等.红毛五加多糖防辐射损伤的观察[J].甘肃中医学院学报,1989, 9(3)：62-63
[115]施亚琴,杨培全,周俊国,等.红毛五加多糖的提取及含量测定[J].华西药学杂志,1994,9(2)：73-74
[116]陈永,卢戌,谭晓晶.红毛五加多糖的分离纯化和表观分子质量测定[J].四川大学学报(自然科学版),2005,42(2)：373-376
[117]赵庆余,袁昌鲁,李铣.红毛五加化学成分的研究[J].中国中药杂志,1991,16(7)：421-424
[118]Kim T. W. Systematics of Eleutherococcus and Related Genera (Araliacea) [M]. Chun-Buk: University of Chun Buk,1997:102-106
[119]Chang S. Y., Yook C. S., Norhara T. Lupane-Triterpene Glycoside from Leaves of Acanthopanax koreanum [J]. Phytochemistry,1999,50 (8):1369-1374
[120]王光荣,王霞文.五味子和刺五加抗衰老作用探讨[J].中药药理与临床,1991,7(6)：31-32
[121]Malyugina L. Influence of Drugs upon the Metastasis of Malignaant tumors [J]. Acta Unio Intern Contra Cancrum,1964,20 (1-2):199-200
[122]谢蜀生,吕秀凤,秦凤华,等.刺五加多糖免疫调节机理初探[J].中华微生物学和免疫学杂志,1989,9(3)：153-155
[123]Zyryanova T. M. Effect of Extracts of Panax, Eleutherococcus, and Leuzea on Blood Supply to the Brain [J]. Stimulyatory Tsent Nerv Sist,1966,6 (1):37-43
[124]徐建国.302种传统治癌中草药水提液对HL-60细胞的诱导分化及细胞毒作用[J].山西医药杂志,1991,20(2)：82-83
[125]单保恩,李巧霞,梁文杰,等.中药五加皮的抗肿瘤作用体内外实验研究[J].中国中西医结合杂志,2004,24(1)：55-58
[126]单保恩,段建萍,张丽华,等.五加皮抗肿瘤活性物质Age对单核细胞产生TNF-a和IL-12的影响[J].中国免疫学杂志,2003,19(7)：490-493
[127]单保恩,付小梅,华正祥,等.中药五加皮提取成分抗肿瘤活性作用机理研究[J].中国中西医结合杂志,2005,25(9)：825-828
[128]陈永,李强,谭晓晶,等.红毛五加多糖的基本性质研究[J].天然产物研究与开发,2004,16(6)：507-510
[129]孔德云,罗思齐.红毛五加化学成分研究[J].中国医药工业杂志,1990,21(5)：202-204
[130]孔德云,金惠芳.红毛五加化学成分研究[J].中国医药工业杂志,1992,23(6)：235-237
[131]颜鸣,刘玉兰,赵余庆.红毛五加不同提取物的药理研究[J].沈阳药学院学报,1991,8(2)：138-139
[132]李成林.红毛五加抗应激作用的实验研究[J].中药药理与临床,1991,7(2)：29-31
[133]张莅峡,董建平,刘泓.红毛五加挥发油成分的GC-MS分析[J].中华中医药杂志,1992,7(1)：41-42
[134]王满霞,张莅峡,刘红,等.红毛五加茎皮挥发油成分对体外培养人白血病粒细胞生物学效应的研究[J].中国中药杂志,1994,19(9)：558-560
[135]张莅峡.红毛五加的研究(内部资料)[M].北京：中国中医研究院基础所,1993：6-8
[136]刘玉兰,颜鸣,王庭,等.红毛五加皮对豚鼠离体心脏冠脉流量及某些心律失常的影响[J].中国中药杂志,1990,15(8)：46-48
[137]沈映君,冷怀瑛,黄国钧,等.红毛五加皮的药理研究[J].成都中医学院院报,1983,6(4)：43-48
[138]曹先兰,李珠莲.刺五加国外实验研究[J].中草药,1980,11(6)：277-283
[139]Brekhman II, Dardymov IV. New Substances of Plant Origin which Increase Nonspecific Resistance [J]. Annual Review of Pharmacology and Toxicology,1969,9 (2):419-430
[140]洪博源.功能性食品刺五加[J].中国林副特产,2006,83(4)：104-104
[141]中国科学院中国植物志编辑委员会.中国植物志[M].北京：科学出版社,1978,54：104-106
[142]马德兹,刘惠兰.宁夏植物志[M].银川：宁夏人民出版社,1986：233-234
[143]王仲礼,刘林德,田国伟,等.短柄五加开花及传粉生物学的研究[J].生物多样性,1997,5(4)：251-256
[144]张晋宁,章英才,任昱坤.宁夏六盘山自然保护区短柄五加分布及生态环境初探[J].宁夏农学院学报,2000,21(1)：81-83
[145]刘庆玲,刘建华,刘金杰.中草药资源开发利用的研究现状与进展[J].生物学通报,2001,36(5)：45-46
[146]Yan H. J., Si L. D., Nai H. W. RAPD Analysis of Natural Populations of Acanthopanax brachypus [J]. Cell Research,1997,7 (1):99-106
[147]岳春雷,江洪,魏伟.辽东栎林下和开阔地上短柄五加(Acanthopanax brachpus)无性系种群生长的比较研[J].生态学报,2001,21(7)：1204-1207
[148]田国伟,王仲礼,刘林德,等.短柄五加种子结构、后熟作用及其细胞化学研究[J].植物分类学报,1998,36(2)：128-133
[149]王仲礼,刘林德,田国伟,等.短柄五加胚和胚乳发育的研究[J].植物研究,1999,19(1)：40-47
[150]王仲礼,刘林德,田国伟,等.短柄五加开花后雌蕊的发育状态与受精作用的研究[J].植物学报,1998,40(4)：309-315
[151]王仲礼,刘林德,田国伟,等.短柄五加大、小孢子发生和雌、雄配子体发育的研究[J].植物研究,1998,18(2)：177-183
[152]卫平.短柄五加茎中氨基酸和金属元素分析[J].中药材,1988,11(6)：37-37
[153]郭小强.甘肃陇东地区野生药用植物资源及开发建议[J].甘肃农业科技,2004,56(2)：49-51
[154]罗金裕,姚树汉.广西五加属药用植物资源调查及其化学成分预试[J].中药材,1993,17(11)：9-11
[155]刘雄,高建德,赵磊,等.太白杜鹃化学成分的初步研究[J].甘肃中医学院学报,2008,23(2)：40-42
[156]国家药典委员会.中华人民共和国药典(第二部)[S].北京：化学工业出版社,2005版,附录：86-102
[157]史志诚.中国草地重要有毒植物[M].北京：中国农业出版社,1997：124-126,267-275
[158]中国医学科学院药物研究所.中草药有效成分的研究(第一分册)[M].北京：人民卫生出版社,1972：9-24
[159]中国科学院上海药物研究所.中草药有效成分提取与分离[M].上海：上海科学技术出版社,1983：9-19
[160]谢心,狄留庆.刺五加叶化学成分和药理作用研究进展[J].中国现代中药,2008,10(11)：6-9
[161]张祎,刘宝珠,裴月湖.刺五加药理作用研究进展[J].沈阳药科大学学报,2002,19(2)：143-146
[162]于家龙,郭丽娜,张长顺.无梗五加化学成分与药理活性的研究进展[J].中国药业,2002,11(2)：73-74
[163]郑应馨,徐恒卫.具有抗肿瘤活性的多糖及其作用机理研究概况[J].中国药师,2003,6(6)：368-673
[164]Lee S. H., Lee Y. S., Jung S. H., et al. Antitumor and Immunostimulating Activities of Acanthopanax sessiliflorus Fruits [J]. Natural Product Sciences,2003,9 (2):112-116
[165]Tapiero H., Tew K.. D., Polyphenols:Do They Play a Role in the Prevention of Human Pathologies [J]. Biomedicine& Pharmacotherapy,2002,56 (4):200-207
[166]中华人民共和国国家标准[M].香料-旋光度的测定(GB／T 14454.5-2008),1-3
[167]中华人民共和国国家标准[M].植物油-碘值的测定(GB／T 5532-1995),255-258
[168]中华人民共和国国家标准[M].精油-酸值的测定(GB／T 14455.5-1993),278-279
[169]中华人民共和国国家标准[M].精油-酯值的测定(GB／T 14455.6-1993),280-281
[170]中华人民共和国国家标准[M].精油-乙醇中溶混度的评估(GB／T 14455.3-1993),272-275
[171]ACIS (Aroma Chemical Information Sevice), ESO-97 Database of Essential oils,1997
[172]Adams R. P., Identification of Essential Oil Components by Gas Chromatography/Mass Spectroscopy [M]. Allured Publishing Corporation, Carol Stream, IL, USA.2001:23-67
[173]Adams R. P., Identification of Essential Oil Components by Gas Chromatography/Mass Spectroscopy (4th ed) [M]. Allured Publishing Corporation, Carol Stream, IL, USA.2007:10-87
[174]Bisio A., Ciarallo G., Romussi G., et al. Chemical Composition of Essential Oils from Some Salvia species [J]. Phytotherapy Research,1998,12 (1):117-120
[175]丛浦珠.质谱学在天然有机化学中的应用[M].北京：科学出版社,1987：158-171
[176]丛浦珠,苏克曼.分析化学手册(第九分册,第二版)[M].北京：化学工业出版社,2000：247-276
[177]Kovats E., Gas Chromatographic Characterization of Organic Substances in the Retention Index System [J]. Advances in Chromatography,1965,1 (2):229-247
[178]Yu J. Q., Lei J. H., Yu H. D., et al. Chemical Composition and Antimicrobial Activity of the Essential Oil of Scutellaria barbata [J]. Phytochemistry,2004,65 (7):881-884
[179]Zhu S. Y., Yang Y, Yu H. D., et al. Chemical Composition and Antimicrobial Activity of the Essential Oils of Chrysanthemum indicum [J]. Journal of Ethnopharmacology,2005,96 (1-2):151-158
[180]马慕英.大蒜抗真菌作用研究[J].食品科学,1993,14(1)：7-11
[181]Tabanca N., Kirimer N., Demirci B., et al. Composition and Antimicrobial Activity of the Essential Oils of Micromeria cristata subsp. phyrgia and the Enantiomeric Distribution of Borneol [J]. Journal of Agricultural and Food Chemistry,2001,49 (9):4300-4303
[182]Tzakou O., Pitarokili D., Chinou I. B., et al. Composition and Antimicrobial Activity of the Essential Oil of Salvia ringens [J]. Planta Medica,2001,67 (1):81-83
[183]谭龙飞,钟彩莲,陈颖姬,等.咖哩粉精油的抗菌作用研究及GC-MS分析[J].中国调味品,2003,28(11)：6-10
[184]Juteau F., Masotti V., Bessiere J. M., et al. Antibacterial and Antioxidant Activities of Artemisia annua Essential Oil [J]. Fitoterapia,2002,73 (6):532-535
[185]夏忠弟,余俊龙.α-蒎烯对白色念珠菌生物合成的影响[J].中国现代医学杂志,2000,10(1)：44-46
[186]廖凤霞,辛龙涛,陈华,等.中药枳实与枳壳挥发油成分对比分析[J].重庆大学学报,2004,27(5)：38-40
[187]Sibanda S., Chigwada G, Poole M., et al. Composition and Bioactivity of the Leaf Essential Oil of Heteropyxis dehniae from Zimbabwe [J]. Journal of Ethnopharmacology,2004,92 (1):107-111
[188]Carson C. F., Riley T. V. Antimicrobial Activity of the Major Components of the Essential Oil of Melaleuca alternifolia [J]. Journal of Applied Bacteriology,1995,78 (3):264-269
[189]Cosentino S., Tuberoso C. I. G., Pisano B., et al. In-vitro Antimicrobial Activity and Chemical Composition of Sardinian thymus Essential Oils [J]. Letters in Applied Microbiology,1999,29 (2): 130-135
[190]王伟江.天然活性单萜—柠檬烯的研究进展[J].中国食品添加剂,2005,16(1)：33-37
[191]陈建伟,李祥,武露凌,等.中国珍稀植物明党参嫩茎叶挥发油化学成分研究[J].天然产物研究与开发,2000,12(3)：48-51
[192]危英,张旭,危莉,等.杏叶防风挥发油化学成分分析[J].贵阳中医学院学报,2005,27(4)：56-57
[193]Marino M., Bersani C., Comi G Impedance Measurements to Study the Antimicrobial Activity of Essential Oils from Lamiaceace and Compositae [J]. International Journal of Food Microbiology, 2001,67(3):187-195
[194]Kashiwada Y., Nonaka G., Nishioka I. Studies on Rhubarb (Rhei Rhizoma). VI. Isolation and Chara-cterization of Stilbenes [J]. Chemical& Pharmaceutical Bulletin,1984,32 (9):3501-3517
[195]Karl C., Muller G., Pedersen P. A. Flavonoideaus Salix alba. Die Strukturdes Terniflorins Undeines Weiteren Acylflavonoides [J]. Phytochemistry,1976,15 (6):1084-1085
[196]Aritomi M. Homoplantaginin, a New Flavonoid Glycoside in Leaves of Plantago asiatica Linnaeus [J]. Chemical& Pharmaceutical Bulletin,1967,15 (4):432-434
[197]Gonzalez A. G., Arteaga J. M., Breton J. L. et al. Five New Labdane Diterpene Oxides from Eupato rium jhanii [J]. Phytochemistry,1977,16 (1):107-110
[198]Ybarra M. I., Popich S., Borkosky S. A., et al. Manoyl Oxide Diterpenoids from Grindelia scorzoner ifolia [J]. Journal of Natural Products,2005,68 (4):554-558
[199]Ahmed A. A., Mahmoud A. A., Ahmed U. M., et al. Manoyl Oxide a-Arabinopyranoside and Grinde lic Acid Diterpenoids from Grindelia integrifolia [J]. Journal of Natural Products,2001,64 (10): 1365-1367
[200]Hamburger M., Hostettmann K. Hydroxycinnamic Acid Esters from Polygala chamaebuxus [J]. Phytochemistry,1985,24 (8):1793-1797
[201]Sakushima A., Coskun M., Tanker M., et al. A Sinapic Acid Ester from Boreava orientalis [J]. Phytochemistry,1994,35 (6):1481-1484
[202]Shan Y. P., Xu L. L., Lu Y., et al. Diterpenes from Coleus forskohlii (Willd.) Briq. (Labiatae) [J]. Chemical& Pharmaceutical Bulletin,2008,56 (1):52-56
[203]Yang X. D., Mei S. X., Zhao J. F., et al. A New Iridoid Glucoside from Lagotis yunnanensis [J]. Chinese Chemical Letters,2003,14 (9):936-938
[204]Xiao K., Xuan L. J, Xu Y. M, et al. Stilbene Glycoside Sulfates from Polygonum cuspidatum [J]. Journal of Natural Products,2000,63 (10):1373-1376
[205]Anjaneyulu A. S. R., Reddy A. V. R., Reddy D. S. K., et al. Pacharin:a New Dibenzo (2,3-6,7) Oxepin Derivative from Bauhinia racemosa Lamk [J]. Tetrahedron,1984,40 (21):4245-4252
[206]Shen Z. L., Zhuo G. L., Jiang X. Z., A Practical Synthesis of trans-Resveratrol [J]. Indian Journal of Chemistry (Section-B),2002,41 (11):2395-2398
[207]Wanjala C. C. W., Majinda R. R. T., A New Stilbene Glycoside from Elephantorrhiza goetzei [J]. Fitoterapia,2001,72 (6):649-655
[208]Gromova A. S., Tyukavkina N. A., Lutskii V. I., et al. Hydroxystilbenes of the Inner Bark of Pinus sibirica [J]. Chemistry of Natural Compounds,1975,11 (6):715-719
[209]Guo W. F., Sakata K., Watanabe N., et al. Geranyl 6-O-β-D-Xylopyranosyl-β-D-Glucopyranoside Isolated as an Aroma Precursor from Tea Leaves for Oolong Tea [J]. Phytochemistry,1993,33 (6): 1373-1375
[210]Kitagawa I., Sakagami M., Hashiuchi F., et al. Apioglycyrrhizin and Araboglycyrrhizin, Two New Sweet Oleanene-Type Triterpene Oligoglycosides from the Root of Glycyrrhiza Inflata [J]. Chemical & Pharmaceutical Bulletin,1989,37 (2):551-553
[211]Hara S., Okabe H., Mihashi K., Gas-Liquid Chromatographic Separation of Aldose Enantiomers as Trimethylsilyl Ethers of Methyl 2-(Polyhydroxyalkyl)-Thiazolidine-4(R)-Carboxylates [J]. Chemical & Pharmaceutical Bulletin,1987,35 (2):501-506
[212]Yang H., Sung S. H., Kim Y. C. Two New Hepatoprotective Stilbene Glycosides from Acer mono Leaves [J]. Journal of Natural Products,2005,68 (1):101-103
[213]Dorman D. E., Roberts J. D. Nuclear Magnetic Resonance Spectroscopy. Carbon-13 Spectra of Some Pentose and Hexose Aldopyranoses [J]. Journal of the American Chemical Society,1970,92 (5): 1355-1361
[214]Kamerling J. P., Bie M. J. A. D., Vliegenthart J. F. G. A PMR Study of the Anomeric Protons in Pertrimethylsilyl Oligosaccharides, a Determination of the Configuration of the Glycosidic Bond [J]. Tetrahedron,1972,28 (11):3037-3047
[215]Yamasaki K., Kasai R., Masaki Y. et al. Application of C-13 NMR to the Structural Elucidation of Acylated Plant Glycosides [J]. Tetrahedron Letters,1977,18 (14):1231-1234
[216]Agrawal P. K., Bansal M. C. Carbon-13 NMR of Flavonoids [M]. Amsterdam:Elsevier,1989:313-315
[217]Laphookhieo S., Karalai C., Ponglimanont C., et al. Pentacyclic Triterpenoid Esters from the Fruits of Bruguiera cylindrica [J]. Journal of Natural Products,2004,67 (5):886-888
[218]Nkengfack A. E., Vouffo T. W., Vardamides J. C., et al. Phenolic Metabolites from Eiythrina Species [J]. Phytochemistry,1997,46 (3):573-578
[219]杨学东,刘江云,许莉,等.蝉冀藤茎中蔗糖酯成分研究[J].高等学校化学学报,2003,24(1)：61-63
[220]Ioset J. R., Marston A., Gupta M. P., et al. Five New Prenylated Stilbenes from the Root Bark of Lonchocarpus chiricanus [J]. Journal of Natural Products,2001,64 (6):710-715
[221]Sridhar C., Subbaraju G. V., Venkateswarlu Y., et al. New Acylated Iridoid Glucosides from Vitex altissima [J]. Journal of Natural Products,2004,67 (12):2012-2016
[222]Chien N. Q., Hung N. V., Santarsiero B. D., et al. New 3-O-Acyl Betulinic Acids from Stiychnosvan prukii Craib [J]. Journal of Natural Products,2004,67 (6):994-998
[223]Zulfiqar A., Tetsuro I., Toshiyuki T., et al. Acetophenone C-Glucosides and Stilbene O-Glucosides in Upuna borneensis [J]. Phytochemistry,2004,65 (14):2141-2146
[224]Takahashi H., Hirata S., Minami H., et al. Triterpene and Flavahone Glycoside from Rhododendron simsii [J]. Phytochemistry,2001,56 (8):875-879
[225]Zou J. H., Yang J. S., Dong Y. S., et al. Flavone C-glycosides from Flowers of Trollius ledebouri [J]. Phytochemistry,2005,66 (10):1121-1125
[226]Zou J. H., Yang J. S., Zhou L. Acylated Flavone C-glycosides from Trollius ledebouri [J]. Journal of Natural Products,2004,67 (4):664-667
[227]尹建元,杨晓红,孟勤,等.刺楸中新七糖皂苷的结构解析[J].分析化学,2004,32(10)：1381-1384
[228]Mimakia Y., Fukushimaa M., Yokosukaa A., et al. Triterpene Glycosides from the Roots of Sanguisorba officinalis [J]. Phytochemistry,2001,57 (5):773-779
[229]姚新生.天然产物化学[M].北京：人民卫生出版社,1994：359-360
[230]Asada Y., Ueoka T., Furuyua T., Novel Acylated Saponins from Montbretia crocosmia(crocosmii flora). Isolation of Saponins and the Structures of Crocosmiosides A, B and H [J]. Chemical& Pharmaceutical Bulletin,1989,37 (8):2139-2146
[231]Zhao J., Nakamura N., Hattori M., et al. New Triterpenoid Saponins from the Roots of Sinocrassula asclepiadea [J]. Chemical& Pharmaceutical Bulletin,2004,52 (2):230-237
[232]路金才,徐菲菲,张新艳.两头尖的化学成分研究[J].药学学报,2002,37(9)：709-712
[233]Sang S. M., Lao A. N., Wang H. C., Two New Spirostanol Saponins from Allium tuberosum [J]. Journal of Natural Products,1999,62 (7):1028-1029
[234]Ishii H., Kitagawa I., Matsushita K., et al. The Configuration and Conformation of the Arabinose Moiety in Platycodins, Saponins Isolated from Platycodon grandiflorum, and Mi-saponins from Madhuca longifolia Based on Carbon-13 and Hydrogen-1 NMR Spectroscopic Evidence:The Total Structures of the Saponins [J]. Tetrahedron Letters,1981,22 (16):1529-1532
[235]Su Y. F., Koike K., Guo D., et al. New Apiose-Containing Triterpenoid Saponins from Conyza blinii [J]. Tetrahedron,2001,57 (31):6721-6726
[236]袁涛,华会明,裴月湖.三棱的化学成分研究(Ⅰ)[J].中草药,2005,36(11)：1607-1609
[237]Harrison L. J., Sia G L., Sim K. Y.5,7-Dihydroxy-8-Methoxyflavone from Tetracera indica [J]. Planta Medica,1994,60 (5):493-494
[238]王红燕,肖丽和,刘丽,等.粘毛黄芩根的化学成分研究[J].沈阳药科大学学报,2003,20(5)：339-341
[239]关玲,权丽辉,徐丽珍,等.广藿香化学成分的研究[J].中国中药杂志,1994,19(6)：356-358
[240]崔艳君,刘屏,陈若芸.鸡血藤有效成分研究[J].中国中药杂志,2005,30(2)：121-123
[241]Genichiro N. A New Class of Dimeric Flavan-3-ol Gallates, the Asinensins A and B, and Proantho-cyanidin Gallates from Green Tea Leaf [J]. Chemical& Pharmaceutical Bulletin,1983,31 (11): 3906-3914
[242]Hanke F. J., Kubo I., Naylor S. Assigning 13C-NMR Resonances of Natural Products [J]. Journal of Natural Products,1989,52(6):1237-1245
[243]Zheng Y. L., Bao J. C., Liu G., et al. Separation and Identification for 4-Hydroxy-3-Methoxy Benzaldehyde from Root of Panax quinquefolius L. [J].中国药学杂志,2006,41(7)：497-500
[244]Patra A., Ghosh G, Sengupta P. K., et al. Carbon-13 NMR Spectral Studies on Chalcones and Acetophenones [J]. Magnetic Resonance in Chemistry,1987,25 (4):734-736
[245]左国营,张志军,陈丽蓉,等.藏药黑蕊虎耳草的化学成分[J],云南植物研究,2005,27(6)：691-694
[246]丁岗,刘延泽,宋毛平,等.诃子中的多元酚类成分[J],中国药科大学学报,2001,32(3)：193-196
[247]Yan K., Lv J. L., Jia A., et al. Studies on Chemical Constituents of Minosa pudica [J]. Chinese Pharmaceutical Journal,2006,41 (16):1293-1295
[248]植飞,孔令义,彭司勋.大蓟化学成分的研究[J].药学学报,2003,38(6)：442-447
[249]Narui T., Sawada K., Takatsuki S., et al. NMR Assignments of Depsides and Tridepsides of the Lichen Family Umbilicariaceae [J]. Phytochemistry,1998,48 (5):815-822
[250]Fujimatu E., Ishikawa T., Kitajima J. Aromatic Compound Glucosides, Alkyl Glucoside and Glucide from the Fruit of Anise [J]. Phytochemistry,2003,63 (5):609-616
[251]黄胜阳,石建功,杨永春,等.藏药旺拉化学成分的研究[J].中国中药杂志,2002,27(2)：118 -120
[252]冯孝章,陈玉武,杨峻山.天麻化学成分的研究[J].化学学报,1979,37(3)：175-182
[253]徐俊平,董亲颐.仙茅化学成分的研究Ⅰ[J].中草药,1986,17(6)：8-10
[254]陈昌祥,倪伟,梅文莉.仙茅根茎中的配糖体[J].云南植物研究,1999,21(4)：521-524
[255]李宁,李铣,杨世林,等.过山蕨总黄酮的化学成分研究(Ⅰ)[J].沈阳药科大学学报,2004,21(2)：105-108
[256]Bernhard H., Josef H. New Chalcones from Bidens pilosa [J]. Planta Medica,1988,54 (1):52-54
[257]王建平,王名洲,高玉敏,等.鬼针草化学成分的研究(Ⅱ)[J].中草药,1995,26(1)：68-70
[258]Ishimaru K., Nishikawa K., Omoto T., et al. Two Flavone 2'-Glucosides from Scutellaria baicalensis [J]. Phytochemistry,1995,40 (1):279-281
[259]Yakushijin K., Tohshima T., Suzuki R., et al. Studies on the Constituents of the Plants of Illicium Species. II. Structures of Phenolic Components [J]. Chemical& Pharmaceutical Bulletin,1983,31 (8):2879-2823
[260]Mattivi F., Reniero F., Korhammer S. Isolation, Characterization, and Evolution in Red Wine Vinification of Resveratrol Monomers [J]. Journal of Agricultural and Food Chemistry,1995,43 (7): 1820-1823
[261]Shim H., Hong W., Ahn Y. Enzymatic Preparation of Phenolic Glucosides by Streptococcus Mutans [J]. Bulletin of the Korean Chemical Society,2003,24 (11):1680-1682
[262]Zhou X., Peng J. Y., Fan G. R., et al. Isolation and Purification of Flavonoid Glycosides from Trollius ledebouri using High-Speed Counter-Current Chromatography by Stepwise Increasing the Flow-Rate of the Mobile Phase [J]. Journal of Chromatography A,2005,1092 (2):216-221
[263]Soltis D. E., Bohm B. A. Flavonoids of Ascarina lucida [J]. Journal of Natural Products,1982,45 (4): 415-417
[264]Wagner H., Iyengar M. A., Beal J. L. Flavone C and O-glycoside in Thalictrum-arten [J]. Phytoche mistry,1971,10 (10):2553-2554
[265]康少文,于永芳,王沛.金莲花化学成分研究[J].中草药,1984,15(6)：7-9
[266]Kin Y. H., Chung B. S., Sankawa U. Pimaradiene Diterpenes from Acanthopanax koreanum [J]. Journal of Natural Products,1988,51 (6):1080-1083
[267]Chung B. S., Kim Y. Phytochemical Investigation of Acanthopanax koreanum, Proc. Inst. Sym. on New Drug Development from Natural Products [C]. Seoul:National University Press,1989:5-7
[268]刘向前,陆昌洙,张承烨.细柱五加皮化学成分的研究[J].中草药,2004,35(3)：250-252
[269]Klass J., Tinto W. F., Mclean S., et al. Friedel and Triterpenoids from Peritassa compta:Complete 1H and 13C Assignments by 2D NMR Spectroscopy [J]. Journal of Natural Products,1992,55 (11): 1626-1630
[270]Nawwar M. A. M., Buddrus J., Brauer H. Dimmer Phenolic Constituents from the Roots of Tamarix nilotica [J]. Phytochemistry,1982,21 (7):1755-1758
[271]Hiroki T., Sueo H., Sansei N. Coumarins from Bark of Fraxinus japonica and F. mandshurica var. japonica [J]. Chemical& Pharmaceutical Bulletin,1985,33 (9):4069-4073
[272]李占林,李铣,李宁,等.沙沂文冠果壳的化学成分[J].沈阳药科大学学报,2005,22(4)：271-273
[273]Takahira M., Kusano A., Shibano M., et al. Piscidic Acid and Fukiic Acid Esters from Cimicifuga simplex [J]. Phytochemistry,1998,49 (7):2115-2119
[274]Sang S., Lapsley K., Jeong W. S., et al. Antioxidative Phenolic Compounds Isolated from Almond Skins (Prunus Amygdalus Batsch) [J]. Journal of Agricultural and Food Chemistry,2002,50 (8): 2459-2463
[275]张雪,高昊,王乃利,等.金钗石斛中的酚性成分[J].中草药,2006,37(5)：652-655
[276]Paulo A., Jimeno M. L., Gomes E. T., et al. Steroidal Alkaloids from Cryptolepis obtusa [J]. Phytoch emistry,2000,53 (3):417-422
[277]李文武,丁立生,李伯刚.苎麻根化学成分的初步研究[J].中国中药杂志,1996,21(7)：427-428
[278]马天波,李孟广.毛白杨叶化学成分的研究[J].中草药,1987,18(3)：105-107
[279]Yano K., Sanada S., Ida Y., et al. Studies on the Constituents of the Bark of Kalopanax pictus Nakai [J]. Chemical& Pharmaceutical Bulletin,1991,39 (4):865-870
[280]Siddiqui S., Hafeez F., Begum S., et al. Oleanderol, A New Pentacyclic Triterpene from the Leaves of Nerium oleander [J]. Journal of Natural Products,1988,51 (2):229-233
[281]Cheminat A., Zawatzky R., Becker H., et al. Caffeoyl Conjugates from Echinacea Species:Structures and Biological Activity [J]. Phytochemistry,1988,27 (9):2787-2794
[282]Kusano A., Shibano M., Kusano G., et al. Studies on the Constituents of Cimicifuga Species XIX. Eight New Glycosides from Cimicifuga simplex Wormsk [J]. Journal of the Chemical& Pharmaceu-tical Bulletin,1996,44 (11):2078-2085
[283]中国科学院上海药物研究所,黄酮体化合物鉴定手册[M].北京：科学出版社,1981：394-620
[284]Markham K. R., Ternai B., Stanley R., et al. Carbon-13 NMR Studies of Flavonoide-III:Naturally Occuring Flavonoid Glycosides and Their Acylated Derivatives [J]. Tetrahedron,1978,34 (9): 1389-1397
[285]许宝安,舒晔,张明哲.金叶子黄酮类成分的研究[J].北京大学学报(自然科学版),1996,32(6)：699-702
[286]刘莹,惠玉虎.刺五加提取物中紫丁香甙、紫丁香树脂甙的HPLC测定法[J].中草药,2000,31(8)：587-588
[287]Gamal A. A. E, Takeya K., Irokawa H., et al. Lignan bis-Glucosides from Galium sinaicum [J]. Phyt-ochemistry,1997,45 (3):597-600
[288]Tung J. H., Lee C. O., Kim Y. C., et al. New Bioactive Cerebrosides from Arisaema amurense [J]. Journal of Natural Products,1996,59 (3):319-322
[289]Yook C. S., Chang S. Y., Lai J. H., Lupne-Glycoside of A. trifoliatus forma tristigmatis Leaves [J], Archives of Pharmacal Research,1999,22 (6):629-632
[290]Miyakoshi M., Shirasuna K., Hirai Y., et al. Triterpenoid Saponins of Acanthopanax nipponicus Leaves [J]. Journal of Natural Products,1999,62 (3):445-448
[291]Zou K., Zhao Y. Y., Tu G. Z., et al. Two Diastereomeric Saponins with Cytotoxic Activity from Albizia julibrissin [J]. Carbohydrate Research,2000,324 (3):182-188
[292]Tommasi N. D., Autore G, Bellino A., et al. Antiproliferative Triterpene Saponins from Trevesia palmata [J]. Journal of Natural Products,2000,63 (3):308-314
[293]Kasai R., Okihara M., Asakawa J., et al. 13C-NMR Study of a and β-Anomeric Pairs of D-mannopyr anosides and L-rhamnopyranosides [J]. Tetrahedron,1979,35 (11):1427-1432
[294]于德泉,杨俊山,周同惠,等.分析化学手册(第七分册,第二版)[M].北京：化学工业出版社,2002：796-798
[295]Kizu H., Shimana H., Tomimori T. Studies on the Constituents of Clematis Species. VI. The Consti-tuents of Clematis stans SIEB. et ZUCC. [J]. Chemical& Pharmaceutical Bulletin,1995,43 (12): 2187-2194
[296]Kang S. S., Kim J. S., Kim Y. H., et al. A Triterpenoid Saponin from Patrinia scabiosaefolia [J]. Journal of Natural Products,1997,60 (10):1060-1062
[297]Chen M., Wu W. W., Dkniel N., et al. Leonticins D-H, Five Triterpene Saponins from Leontice kiangnanensis [J]. Phytochemistry,1997,44 (3):497-504
[298]Shao C. J., Kasai R., Xu J. D., et al. Saponins from Leaves of Acanthopanax senticosus Harms, Ciwujia. II. Structures of Ciwujianosides A1, A2, A3, A4 and D3 [J]. Chemical& Pharmaceutical Bulletin,1989,37 (1):42-45
[299]黄儒强,郭子杰,邓伟玲,等.田七中总黄酮含量的测定及其黄酮种类的初步鉴别[J].现代食品科技,2007,23(3)：66-68
[300]陈孝泉.植物化学分类学[M].北京：高等教育出版社,1990：53-79
[301]谢鹏,张敏红.黄酮类化合物抑菌作用的研究进展[J].中国动物保健,2004,6(12)：35-37
[302]Wei H. C., Cai Q. Y, Rahn R. O., et al. Inhibition of UV Light and Fenton Reaction Induced Oxda-tive DNA Damage by the Soybean Isoflavone Genistein [J]. Carcinogenesis,1996,17 (1):73-78
[303]Masahiro K., Naoki I., Hiroichi N. Effects of Luteolin and Other Flavonoids on IgE-Mediated Allergic Reactions [J]. Planta Medica,2000,66 (1):25-29
[304]睢大赞,吕忠智,于晓凤.刺五加叶皂甙的抗实验性心律失常作用[J].中草药,1997,28(2)：99-101
[305]Francis G., Zohar K., Harinder P. S., et al. The Biological Action of Saponins in Animal Systems:A Review [J]. British Journal of Nutrition,2002,88 (6):587-605
[306]冯雪梅,叶红军,韩登春,等.刺五加叶皂甙对动物实验性肝癌抑制作用的研究[J].中国老年学杂志,1999,9(5)：298-299
[307]胡宗礼,黄晓萍.倒卵叶五加总皂苷对心肌缺血再灌注大鼠左心功能和血清一氧化氮及血浆内槲皮素的影响[J].中国现代应用药学,2003,20(6)：468-470
[308]陈小川,夏兰,胡松,等.短毛五加总皂苷在体外抑制人血小板聚集和血小板因子4释放[J].中国药理学报(英文),1996,17(6)：523-526
[309]Strickland F. M., Pelley R. P., Kripke M. L. Prevention of Ultraviolet Radiation-Induced Suppression of Contact and Delayed Hypersensitivity by Aloe barbadensis Gel Extract [J]. Journal of Investiga-tive Denmatology,1994,102(2):197-204
[310]Yagi A., Egusa T., Arase M. et al.. Isolation and Characterization of the Glycoprotein Fraction with a Proliferation-Promoting Activity on Human and Hamster Cells in vitro from Aloe vera Gel [J]. Planta Medica,1997,63 (1):18-21
[311]Hart L. A. T., Vanden Berg A. J. J., Kuis L. An Anti-Complementary Polysaccharide with Immunological Adjuvant Activity from the Leaf Parenchyma Gel of Aloe vera [J]. Planta Medica, 1989,55 (6):509-512
[312]Chen M. L., Song F. R., Guo M. Q., et al. Analysis of Flavonoid Constituents from Leaves of Acanthopanax senticosus Harms by Electrospray Tandem Mass Spectrometry [J]. Rapid Communi-cations in Mass Spectrometry,2002,16 (4):264-271
[313]Shi B.J., Nie X. H., Chen L. Z., et al. Anticancer Activities of a Chemically Sulfated Polysaccharide Obtained from Grifola frondosa and Its Combination with 5-Fluorouracil against Human Gastric Carcinoma Cells [J]. Carbohydrate Polymers,2007,68 (4):687-692
[314]刘芳,杨翠军,孙黎,等.五加皮多糖对人宫颈癌Hela细胞凋亡作用的研究[J],时珍国医国药,2009,20(5)：1178-1179
[315]刘国龙,毛海波,伍勇,等.红毛五加多糖对荷人喉癌裸鼠抗癌作用的研究[J],时珍国医国药,2008,19(8)：1964-1965
[316]Lee S. H., Lee Y. S., Jung S. H., et al. Antitumor and Immunostimulating Activities of Acanthopanax sessiliflorus Fruits [J]. Natural Product Sciences,2003,9 (2):112-116.
[317]唐兴文,董钰明,李春兰,等.红毛五加总苷和多糖药理作用研究进展[J].兰州医学院学报,2004,30(4)：93-96
[318]张惟杰.糖复合物生化研究技术[M].杭州：浙江大学出版社,1999：78-79
[319]韩公羽,沈企华.植物药有效成分的研究与开发[M].杭州：杭州大学出版社,1991：102-103
[2]郑万均.中国树木志[M].北京：中国林业出版社,1985,2：1777-1780
[3]刘立品.子午岭木本植物志[M].兰州：兰州大学出版社,1998：290-291
[4]Yook C. S. Medicinal Herbs of Acanthopanax in Asia [M]. Seoul:Kyung-Won Media,2001:132-145
[5]Kim C. H., Sun B. Y., New Taxa and Combinations in Eleutherococcus (Araliacaea) from Eastern Asia [J]. Novan,2000,10 (3):209-214
[6]倪娜,刘向前.五加科五加属植物的研究进展[J].中草药,2006,37(12)：1895-1900
[7]赵余庆,袁昌鲁,杨志强.红毛五加挥发性成分的研究[J].中药材,1992,15(4)：28-31
[8]倪娜,赵君.GC-MS法分析红毛五加皮中挥发油的化学成分[J].化学,2007,2(11)：852-855
[9]张莅峡,刘泓,董建平.毛梗红毛五加皮挥发油化学成分的研究[J].中国药学杂志,1994,29(2)：83-86
[10]张莅峡,刘泓.红毛五加茎皮、叶、果实挥发油的GC-MS比较分析[J].中国中医基础医学杂志,2001,7(5)：24-27
[11]于万滢,张华,黄威东.多种气相色谱联用技术分析陕西刺五加茎挥发油的化学成分[J].色谱,2005,23(2)：196-201
[12]Yu W. Y., Zhang H., Huang W. D., et al. Analysis of the Volatile Oil from the Stem of Acanthopanax Senticosus (Rupr. et Maxim.) Harms with Several Hyphenated Methods of Chromatography [J]. Frontiers of Chemistry in China,2006,1 (2):193-198
[13]邢有权,孙志忠,韩晓玲,等.黑龙江刺五加根和茎挥发油成分的气相色谱-质谱分析[J].黑龙江大学学报(自然科学版),1992,9(4)：92-96
[14]薛兴亚,于万滢,陈吉平,等.气相色谱保留参数用于刺五加果挥发油中萜烯及萜烯氧化物的分类[J].色谱,2005,23(4)：422-425
[15]何方奕,李铁纯,李发美.气相色谱-质谱法分析无梗五加茎的挥发油成分[J].质谱学报,2004,25(2)：103-106
[16]刘向前,张承烨,印文教,等.细柱五加的挥发油成分分析[J].中草药,2001,32(12)：1074-1075
[17]Muselli A., Hoi T. M., Cu L. D., et al. Composition of the Essential Oil of Acanthopanax trifoliatus (L.) Merr. (Araliacaea) from Vietnam [J]. Flavour and Fragrance Journal,1999,14 (1):41-44
[18]Kiem P. V., Cai X. F., Minh C. V., et al. Lupane-Triterpene Carboxylic Acids from the Leaves of Acanthopanax trifoliatus [J]. Chemical& Pharmaceutical Bulletin,2003,51 (12):1432-1435
[19]刘向前,张承烨,陆昌洙.细柱五加叶中羽扇豆烷型三萜成分[J].兰州大学学报(自然科学版),2006,42(4)：86-91
[20]Yook C. S., Liu X. Q., Chang S. Y., et al. Lupane-Triterpene Glycosides from the Leaves of Acantho-panax gracilistylus [J]. Chemical& Pharmaceutical Bulletin,2002,50 (10):1383-1385
[21]Liu X. Q., Chang S. Y., Park S. Y., et al. Studies on the Constituents of the Stem Barks of Acanthopan ax gracilistylus W. W. Smith [J]. Natural product Science,2002a,50 (8):23-25
[22]Yook C. S., Kim I. H., Hahn D. R., et al. A Lupane-Triterpene Glycoside from Leaves of Two Acantho panax [J]. Phytochemistry,1998,49 (3):839-843
[23]Yook C. S., Chang S. Y., Lai J. H., et al. Lupane-Glycoside of Acanthopanax trifoliatus forma tristig matis Leaves [J]. Archives of Pharmacal Research,1999,22 (6):629-632
[24]Liu X. Q., Chang S. Y., Park S. Y. et al. A New Lupane-Triterpene Glycoside from the Leaves of Acanthopanax gracilistylus [J]. Archives of Pharmacal Research,2002,25 (6):831-836
[25]Chang S. Y., Yook C. S., Nohara T. Two New Lupane-Triterpene Glycosides from Leaves of Acantho panax koreanum [J]. Chemical& Pharmaceutical Bulletin,1998,46 (1):163-165
[26]Cai X. F., Lee I. S., Shen G. H., et al. Triterpenoids from Acanthopanax koreanum Root and Their Inhi bitory Activities on NFAT Transcription [J]. Archives of Pharmacal Research,2004,27 (8):825-828
[27]Chang S. Y., Yook C. S., Nohara T. Lupane-Triterpene Glycosides from Leaves of Acanthopanax koreanum [J]. Phytochemistry,1999,50 (8):1369-1374
[28]Choi H. S., Kim H. J., Nam S. G., et al. Lupane Glycosides from the Leaves of Acanthopanax korean um [J]. Chemical& Pharmaceutical Bulletin,2008,56 (11):1613-1616
[29]Ty Ph. D., Lischewski M., Phiet H. V., et al. Two Triterpenoid Carboxylic Acids from Acanthopanax trifoliatus [J]. Phytochemistry,1984,23 (12):2889-2891
[30]Ty P. D., Lischewski M., Phiet H. V., et al.3a,1 la-Dihydroxy-23-oxo-Lup-20(29)-en-28-oic Acid from Acanthopanax trifoliatus [J]. Phytochemistry,1985,24 (4):867-869
[31]Oh O. J., Chang S. Y., Yook C. S., et al. Two 3,4-seco-Lupane Triterpenes from Leaves of Acanthopan ax divaricatus var. albeofructus [J]. Chemical& Pharmaceutical Bulletin,2000,48 (6):879-881
[32]Won J. H., Park S. Y., Nam S. G., et al. Inhibition of Lipopolysaccharide-Induced Expression of Inducible Nitric Oxide and Cyclooxygenase-2 by Chiisanoside via Suppression of Nuclear Factor-KB Activation in RAW 264.7 Macrophage Cells [J]. Biological& Pharmaceutical Bulletin,2005,28 (10): 1919-1924
[33]Park S. Y., Chang S. Y., Yook C. S., et al. New 3,4-seco-Lupane-Type Triterpene Glycosides from Acanthopanax senticosus forma inermis [J]. Journal of Natural Products,2000,63 (12):1630-1633
[34]Shirasuna K., Miyakoshi M., Mimoto S., et al. Lupane Triterpenoid Glycosyl Esters from Leaves of Acanthopanax divaricatus [J]. Phytochemistry,1997,45 (3):579-584
[35]Hahn D. R., Kasai R., Kim J. H., et al. A New Glycosyl Ester of a 3,4-seco-Triterpene from Korean Medicinal Plant, Acanthopanax chiisanensis (Araliaceae) [J]. Chemical& Pharmaceutical Bulletin, 1984,32 (3):1244-1247
[36]Kasai R., Matsumoto K., Taniyasu S., et al.3,4-seco-Lupane-Type Triterpene Glycosyl Esters from a Korean Medicinal Plant, Acanthopanax chiisanensis (Araliaceae) [J]. Chemical& Pharmaceutical Bulletin,1986,34 (8):3284-3289
[37]安琪,杨春娟,宋洋,等.无梗五加果化学成分的研究[J].天然产物研究与开发,2008,20(5)：765-769
[38]王广树,陈燕萍,徐景达,等.无梗五加叶化学成分的研究[J].中国药学杂志,1997,32(1)：11-13
[39]Yoshizumi K., Hirano K., Ando H., et al. Lupane-Type Saponins from Leaves of Acanthopanax sessiliflorus and Their Inhibitory Activity on Pancreatic Lipase [J]. Jouranal of Agricultural and Food Chemistry,2006,54 (2):335-341
[40]Lischewski M., Ty P. D., Kutschabsky L., et al. Two 24-nor-Triterpenoid Carboxylic Acids from Acanthopanax trifoliatus [J]. Phytochemistry,1985,24 (10):2355-2357
[41]Park S. Y., Yook C. S., Nohara T. A Novel 3,4-seco-Migrated-Lupane Glycoside with a Seven-Memb ered B-ring from Acanthopanax divaricatus var. sachunensis [J]. Tetrahedron Letters,2001,42 (15): 2825-2828
[42]赵余庆,吴立军,李铣.刺五加活性成分Liriodendrin的结构研究[J].中草药,1990,21(3)：44-45
[43]Wenhong J., Wei L., Likun H., et al. Biologically Active Triterpenoid Saponins from Acanthopanax senticosus [J]. Journal of Natural Products,2006,69 (11):1577-1581
[44]Park S. Y, Choi H. S., Yook C. S., et al. A New Lupane Glycoside from the Leaves of Acanthopanax koreanum [J]. Chemical& Pharmaceutical Bulletin,2005,53 (1):97-99
[45]Kiem P. V., Minh C. V., Cai X. F., et al. A New 24-nor-Lupane-Glycoside of Acanthopanax trifoliatus [J]. Archives of Pharmacal Research,2003,26 (9):706-708
[46]Yang C. J., An Q., Xiong Z. L., et al. Triterpenes from Acanthopanax sessiliflorus Fruits and Their Antiplatelet Aggregation Activities [J]. Planta Medica,2009,75 (6):656-659
[47]Park S. Y., Chang S. Y., Oh O. J., et al. nor-Oleanene Type Triterpene Glycosides from the Leaves of Acanthopanax japonicus [J]. Phytochemistry,2002,59 (4):379-384
[48]Shao C. J., Kasai R., Xu J. D., et al. Saponins from Leaves of Acanthopanax senticosus Harms., Ciwujia. II.:Structures of Ciwujianosides A1, A2, A3, A4 and D3 [J]. Chemical& Pharmaceutical Bulletin,1989,37 (1):42-45
[49]Shao C. J., Kasai R., Xu J. D., et al. Saponins from Leaves of Acanthopanax senticosus Harms, Ciwujia:Structures of Ciwujianosides B, C1, C2, C3, C4, D1, D2 and E [J]. Chemical& Pharmaceutical Bulletin,1988,36 (2):601-608
[50]Miyakoshi M., Shirasuna K., Hirai Y, et al. Triterpenoid Saponins of Acanthopanax nipponicus Leaves [J]. Journal of Natural Products,1999,62 (3):445-448
[51]史良久.刺五加化学成分的研究[J].药学通报,1981,16(3)：53-56
[52]Miyakoshi M., Ida Y, Isoda S., et al.3-epi-Oleanene-Type Triterpene Glycosyl Esters from Leaves of Acanthopanax spinosus [J]. Phytochemistry,1993,33 (4):891-895
[53]Miyakoshi M., Ida Y, Isoda S., et al.3a-Hydroxy-Oleanane-Type Triterpene Glycosyl Esters from Leaves of Acanthopanax spinosus [J]. Phytochemistry,1993,34 (6):1599-1602
[54]Miyakoshi M., Isoda S., Sato H., et al.3a-Hydroxy-Oleanene Type Triterpene Glycosyl Esters from Leaves of Acanthopanax spinosus [J]. Phytochemistry,1997,46 (7):1255-1259
[55]张杰,李文,高慧媛,等.刺五加种子的化学成分[J].沈阳药科大学学报,2005,22(3)：183-186
[56]Kohda H., Tanaka S., Yamaoka Y. Saponins from Leaves of Acanthopanax hypoleucus Makino [J]. Chemical& Pharmaceutical Bulletin,1990,38 (12):3380-3383
[57]Li F., Li W., Fu H. W., et al. Pancreatic Lipase-Inhibiting Ttriterpenoid Ssaponins from Fruits of Acanthopanax senticosus [J]. Chemical& Pharmaceutical Bulletin,2007,55 (7):1087-1089
[58]程东亮,邵宇,杨立.红毛五加叶的三萜皂甙[J].植物学报,1994,36(1)：75-79
[59]孔德云,罗思弃.红毛五加化学成分研究Ⅰ[J].中国医药工业杂志,1990,21(5)：202-205
[60]Sawada H., Miyakoshi M., Isoda S., et al. Saponins from Leaves of Acanthopanax sieboldianus [J]. Phytochemistry,1993,34(4):1117-1121
[61]孔德云,金惠芳,罗思弃.红毛五加化学成分研究Ⅱ[J].中国医药工业杂志,1992,23(5)：215-217
[62]Park S. Y., Yook C. S., Nohara T. New Oleanene Glycosides from the Leaves of Acanthopanax japonicus [J]. Chemical& Pharmaceutical Bulletin,2005,53 (9):1147-1151
[63]刘向前,金寅钐,陆昌洙,等.异柱五加叶中齐墩果烷型皂苷的研究[J].天然产物研究与开发,2008,20(1)：66-69
[64]Nishibe S., Kinoshita H., Takeda H., et al. Phenolic Compounds from Stem Bark of Acanthopanax senticosus and Their Pharmacological Effect in Chronic Swimming Stressed Rats [J]. Chemical& Pharmaceutical Bulletin,1990,38 (6):1763-1765
[65]卫平,于高麦.短柄五加化学成分研究[J].中草药,1989,20(4)：8-10
[66]孙晓云.蜀五加化学成分的研究[J].天然产物研究与开发,2001,13(2)：34-68
[67]Elyakova L. A., Elyakov G. B. Glycosides from Roots of Acanthopanax sessiliflorum [J]. Russian Chemical Bulletin,1965,14 (3):537-539
[68]Kim J. H., Hahn D. R. Studies on the Chemical Constituents of Acanthopanax chiisanensis Nakai Roots [J]. Archives of Pharmacal Research,1981,4 (1):59-62
[69]Cai X. F., Lee I. S., Dat N. T., et al. Inhibitory Lignans against NEAT Transcription Factor from Acanthopanax koreanum [J]. Archives of Pharmacal Research,2004,27 (7):738-741
[70]赵余庆,杨松松,柳江华,等.刺五加化学成分的研究[J].中国中药杂志,1993,18(7)：428-429
[71]郭丽娜,吴立军,郭祥菊,等.无梗五加茎叶化学成分的研究[J].中草药,2006,37(6)：833-834
[72]吴立军,郑健,姜宝虹,等.刺五加茎叶化学成分[J].药学学报,1999,34(4)：294-296
[73]向仁德,徐任生.南五加皮化学成分的研究[J].植物学报,1983,25(4)：356-362
[74]刘向前,陆昌洙,张承烨.细柱五加化学成分的研究[J].中草药,2004,35(3)：250-252
[75]Sanghyun L., Jun J., Kuk H. S., et al. Sessiline, A New Nitrogenous Compound from the Fruits of Acanthopanax sessiliflorus [J]. Planta Medica,2002,68 (10):939-941
[76]Ban H. S., Lee S. H., Kim Y. P., et al. Inhibition of Prostaglandin E2 Production by Taiwanin C Isolatedfrom the Root of Acanthopanax chiisanensis and the Mechanism of Action [J]. Biochemical Pharmacology,2002,64 (9):1345-1354
[77]Yoo H. H., Lee S. H., Jin C. B., et al. Mechanism-Based Inactivation of Cytochrome P4503A4 by Methylenedioxyphenyl Lignans from Acanthopanax chiisanensis [J]. Planta Medica,2008,74 (8):
808-813
[78]Kim Y. H., Chung B. S., Ko Y. S., et al. Studies on the Chemical Constituents of Acanthopanax koreanum (Ⅱ) [J]. Archives of Pharmacal Research,1988,11 (2):159-162
[79]Elyakova L. A., Dzizenko A. K., Sova V. V., et al. (-)-Sesamin and (-)-Sainine from Acanthopanax sessiliflorus and Their NMR Spectra [J]. Chemistry of Natural Compounds,1966,2 (3):117-119
[80]吴立军,阮丽军,郑健.刺五加茎叶化学成分研究[J].药学学报,1999,34(11)：839-841
[81]苑艳光,王录全,吴立军,等.刺五加茎的化学成分[J].沈阳药科大学学报,2002,19(5)：325-327
[82]Zhou X. G., Zheng C. Y., Huang J. S., et al. Identification of Herb Acanthopanax senticosus (Rupr. Et Maxim.) Harms by Capillary Electrophoresis with Electrochemical Detection [J]. Analytical Sciences, 2007,23 (6):705-711
[83]韩莹,李遇伯,崔兰冲.HPLC法同时测定无梗五加果中东莨菪内酯和异嗪皮啶的含量[J].沈阳药科大学学报,2006,23(9)：73-576
[84]Won C. K. Study on the Constituents of Seeds of Acanthopanax sessiliflorum Seemann Ⅰ[J]. Saengyak Hakhoechi,1987,18 (3):184-187
[85]赵余庆,柳江华,赵光燃.刺五加中脂肪酸类和酯类成分的分离与鉴定[J].中医药学报,1989,15(3)：55-56
[86]Yook C. S., Oho L. P., et al. Study on the Constituents in the Root Barks of Acanthopanax sessiliflor us Seemann Ⅱ [J]. Saegyak Hakhoechi,1997,28 (1):31-34
[87]Asilbekova D. T. Gusakova S. D., Glushenkova A. I. Lipids of the Fruits of Acanthopanax sessilifl orus [J]. Chemistry of Natural Compounds,1985,21 (6):719-724
[88]Asilbekova D. T., Gusakova S. D., Glushenkova A. I. New Hydroxy Fatty Acids of Acanthopanax sessiliflorus [J], Chemistry of Natural Compounds,1991,27 (5):542-544
[89]Kiem P. V., Cai X. F., Minh C. V., et al. Kaurane-Type Diterpene Glycoside from the Stem Bark of Acanthopanax trifoliatus [J]. Planta Medica,2004,70 (3):282-284
[90]Kim Y. H., Kim H. S., Lee S. W., et al. Kaurane Derivatives from Acanthopanax koreanum [J]. Phytochemistry,1995,39 (2):449-451
[91]Cai X. F., Shen G. H., Dat N. T., et al. Inhibitory Effect of Kaurane-Type Diterpenoids from Acantho panax koreanum on TNF-a Secretion from Trypsin-Stimulated HMC-1 Cells [J]. Archives of Pharma-cal Research,2003,26 (9):731-734
[92]Na M. K., Oh W. K., Kim Y. H., et al. Inhibition of Protein Tyrosine Phosphatase 1B by Diterpenoids Isolated from Acanthopanax koreanum [J]. Bioorganic& Medicinal Chemistry Letters,2006,16 (11): 3061-3064
[93]Cai X. F., Shen G. H., Dat N. T., et al. Inhibitory Effect of TNF-α and IL-8 Secretion by Pimarane-Type Diterpenoids from Acanthopanax koreanum [J]. Chemical& Pharmaceutical Bulletin,2003,51 (5):605-607
[94]Kim Y. H., Chung B. S., Sankawa U. Pimaradiene Diterpenes from Acanthopanax koreanum [J]. Journal of Natural Products,1988,51 (6):1080-1083
[95]Phuong N. T., Lee K. A., Jeong S. J., et al. Capillary Electrophoretic Method for the Determination of Diterpenoid Isomers in Acanthopanax Species [J]. Journal of Pharmaceutical and Biomedical Analysis, 2006,40 (1):56-61
[96]Miyakoshi M., Tajima Y., Isoda S., et al. Constituents from the Leaves of Acanthopanax tricodon [J]. Nature Medicine,1997,51 (5):494-497
[97]陈貌连,宋凤瑞,郭明全,等.刺五加叶中黄酮类化合物的结构鉴定[J].高等学校化学学报,2002,23(5)：805-808
[98]Zhao W. M., Qin G. W., Xu R. S., et al. Constituents from the Roots of Acanthopanax setchuenensis [J]. Fitoterapia,1999,70 (5):529-531
[99]Feng S. L., Hu F. D., Zhao J. X., et al. Determination of Eleutheroside E and Eleutheroside B in Rat Plasma and Tissue by High-Performance Liquid Chromatography using Solid-Phase Extraction and Photodiode Array Detection [J]. European Journal of Pharmaceutics and Biopharmaceutics,2006,62 (3):315-320
[100]Jung H. J., Park H. J., Kim R. G., et al. In vivo Anti-Inflammatory and Antinociceptive Effects of Liriodendrin Isolated from the Stem Bark of Acanthopanax senticosus [J]. Planta Medica,2003,69 (7):610-616
[101]王子灿,乔善义,马安德,等.高效液相色谱-质谱联用技术分析刺五加抗疲劳化学成分[J].第一军医大学学报,2003,23(4)：355-357
[102]Fang J. N., Proksch A., Wagner H. Immunologically Active Polysaccharides of Acanthopanax senticosus [J]. Phytochemistry,1985,24 (11):2619-2622
[103]欧惠英,李廷昊,东美麟,等.吴茱萸五加化学成分的研究[J].中草药,1994,25(12)：625-625
[104]孔令义,邵春杰,徐景达.无梗五加根化学成分的研究[J].中草药,1988,19(11)：482-486
[105]斯建勇,陈迪华,常琪,等.倒卵叶五加根茎的化学成分[J].植物学报,1993,35(6)：483-485
[106]Fang J. N. Immunologically Active Polysaccharides of Acanthopanax senticosus [J]. Phytochemistry, 1995,24(11):2619-2622
[107]刘起华,朱札,李文兰.刺五加主要活性成分化学与药理研究[J].时珍国医国药,1999,10(5)：305-308
[108]李筱玲,邓寒霜.刺五加茎叶化学成分分析[J].商洛师范专科学校学报,2006,20(1)：103-105
[109]张丽萍,陈丽颖,庄会科,等.刺五加果化学成分的分析[J].东北师范大学学报(自然科学版),1992,24(1)：120-128
[110]金同顺,欧惠英.3种五加中微量元素和氨基酸含量分析[J].南京师范大学学报(自然科学版),1995,18(2)：45-49
[111]杨坡,孙宝俊,刘娥,等.短梗五加食药用价值及开发利用[J].中国野生植物资源,2006,25(2)：43-44
[112]郭丽娜.无梗五加化学成分与药理活性的研究进展[J].中国药业,2002,11(12)：73-74
[113]杨勤福,窦忠健.无梗五加的研究[J].中医研究,1999,12(6)：14-15
[114]李兴玉,魏虎来,张吉旺,等.红毛五加多糖防辐射损伤的观察[J].甘肃中医学院学报,1989, 9(3)：62-63
[115]施亚琴,杨培全,周俊国,等.红毛五加多糖的提取及含量测定[J].华西药学杂志,1994,9(2)：73-74
[116]陈永,卢戌,谭晓晶.红毛五加多糖的分离纯化和表观分子质量测定[J].四川大学学报(自然科学版),2005,42(2)：373-376
[117]赵庆余,袁昌鲁,李铣.红毛五加化学成分的研究[J].中国中药杂志,1991,16(7)：421-424
[118]Kim T. W. Systematics of Eleutherococcus and Related Genera (Araliacea) [M]. Chun-Buk: University of Chun Buk,1997:102-106
[119]Chang S. Y., Yook C. S., Norhara T. Lupane-Triterpene Glycoside from Leaves of Acanthopanax koreanum [J]. Phytochemistry,1999,50 (8):1369-1374
[120]王光荣,王霞文.五味子和刺五加抗衰老作用探讨[J].中药药理与临床,1991,7(6)：31-32
[121]Malyugina L. Influence of Drugs upon the Metastasis of Malignaant tumors [J]. Acta Unio Intern Contra Cancrum,1964,20 (1-2):199-200
[122]谢蜀生,吕秀凤,秦凤华,等.刺五加多糖免疫调节机理初探[J].中华微生物学和免疫学杂志,1989,9(3)：153-155
[123]Zyryanova T. M. Effect of Extracts of Panax, Eleutherococcus, and Leuzea on Blood Supply to the Brain [J]. Stimulyatory Tsent Nerv Sist,1966,6 (1):37-43
[124]徐建国.302种传统治癌中草药水提液对HL-60细胞的诱导分化及细胞毒作用[J].山西医药杂志,1991,20(2)：82-83
[125]单保恩,李巧霞,梁文杰,等.中药五加皮的抗肿瘤作用体内外实验研究[J].中国中西医结合杂志,2004,24(1)：55-58
[126]单保恩,段建萍,张丽华,等.五加皮抗肿瘤活性物质Age对单核细胞产生TNF-a和IL-12的影响[J].中国免疫学杂志,2003,19(7)：490-493
[127]单保恩,付小梅,华正祥,等.中药五加皮提取成分抗肿瘤活性作用机理研究[J].中国中西医结合杂志,2005,25(9)：825-828
[128]陈永,李强,谭晓晶,等.红毛五加多糖的基本性质研究[J].天然产物研究与开发,2004,16(6)：507-510
[129]孔德云,罗思齐.红毛五加化学成分研究[J].中国医药工业杂志,1990,21(5)：202-204
[130]孔德云,金惠芳.红毛五加化学成分研究[J].中国医药工业杂志,1992,23(6)：235-237
[131]颜鸣,刘玉兰,赵余庆.红毛五加不同提取物的药理研究[J].沈阳药学院学报,1991,8(2)：138-139
[132]李成林.红毛五加抗应激作用的实验研究[J].中药药理与临床,1991,7(2)：29-31
[133]张莅峡,董建平,刘泓.红毛五加挥发油成分的GC-MS分析[J].中华中医药杂志,1992,7(1)：41-42
[134]王满霞,张莅峡,刘红,等.红毛五加茎皮挥发油成分对体外培养人白血病粒细胞生物学效应的研究[J].中国中药杂志,1994,19(9)：558-560
[135]张莅峡.红毛五加的研究(内部资料)[M].北京：中国中医研究院基础所,1993：6-8
[136]刘玉兰,颜鸣,王庭,等.红毛五加皮对豚鼠离体心脏冠脉流量及某些心律失常的影响[J].中国中药杂志,1990,15(8)：46-48
[137]沈映君,冷怀瑛,黄国钧,等.红毛五加皮的药理研究[J].成都中医学院院报,1983,6(4)：43-48
[138]曹先兰,李珠莲.刺五加国外实验研究[J].中草药,1980,11(6)：277-283
[139]Brekhman II, Dardymov IV. New Substances of Plant Origin which Increase Nonspecific Resistance [J]. Annual Review of Pharmacology and Toxicology,1969,9 (2):419-430
[140]洪博源.功能性食品刺五加[J].中国林副特产,2006,83(4)：104-104
[141]中国科学院中国植物志编辑委员会.中国植物志[M].北京：科学出版社,1978,54：104-106
[142]马德兹,刘惠兰.宁夏植物志[M].银川：宁夏人民出版社,1986：233-234
[143]王仲礼,刘林德,田国伟,等.短柄五加开花及传粉生物学的研究[J].生物多样性,1997,5(4)：251-256
[144]张晋宁,章英才,任昱坤.宁夏六盘山自然保护区短柄五加分布及生态环境初探[J].宁夏农学院学报,2000,21(1)：81-83
[145]刘庆玲,刘建华,刘金杰.中草药资源开发利用的研究现状与进展[J].生物学通报,2001,36(5)：45-46
[146]Yan H. J., Si L. D., Nai H. W. RAPD Analysis of Natural Populations of Acanthopanax brachypus [J]. Cell Research,1997,7 (1):99-106
[147]岳春雷,江洪,魏伟.辽东栎林下和开阔地上短柄五加(Acanthopanax brachpus)无性系种群生长的比较研[J].生态学报,2001,21(7)：1204-1207
[148]田国伟,王仲礼,刘林德,等.短柄五加种子结构、后熟作用及其细胞化学研究[J].植物分类学报,1998,36(2)：128-133
[149]王仲礼,刘林德,田国伟,等.短柄五加胚和胚乳发育的研究[J].植物研究,1999,19(1)：40-47
[150]王仲礼,刘林德,田国伟,等.短柄五加开花后雌蕊的发育状态与受精作用的研究[J].植物学报,1998,40(4)：309-315
[151]王仲礼,刘林德,田国伟,等.短柄五加大、小孢子发生和雌、雄配子体发育的研究[J].植物研究,1998,18(2)：177-183
[152]卫平.短柄五加茎中氨基酸和金属元素分析[J].中药材,1988,11(6)：37-37
[153]郭小强.甘肃陇东地区野生药用植物资源及开发建议[J].甘肃农业科技,2004,56(2)：49-51
[154]罗金裕,姚树汉.广西五加属药用植物资源调查及其化学成分预试[J].中药材,1993,17(11)：9-11
[155]刘雄,高建德,赵磊,等.太白杜鹃化学成分的初步研究[J].甘肃中医学院学报,2008,23(2)：40-42
[156]国家药典委员会.中华人民共和国药典(第二部)[S].北京：化学工业出版社,2005版,附录：86-102
[157]史志诚.中国草地重要有毒植物[M].北京：中国农业出版社,1997：124-126,267-275
[158]中国医学科学院药物研究所.中草药有效成分的研究(第一分册)[M].北京：人民卫生出版社,1972：9-24
[159]中国科学院上海药物研究所.中草药有效成分提取与分离[M].上海：上海科学技术出版社,1983：9-19
[160]谢心,狄留庆.刺五加叶化学成分和药理作用研究进展[J].中国现代中药,2008,10(11)：6-9
[161]张祎,刘宝珠,裴月湖.刺五加药理作用研究进展[J].沈阳药科大学学报,2002,19(2)：143-146
[162]于家龙,郭丽娜,张长顺.无梗五加化学成分与药理活性的研究进展[J].中国药业,2002,11(2)：73-74
[163]郑应馨,徐恒卫.具有抗肿瘤活性的多糖及其作用机理研究概况[J].中国药师,2003,6(6)：368-673
[164]Lee S. H., Lee Y. S., Jung S. H., et al. Antitumor and Immunostimulating Activities of Acanthopanax sessiliflorus Fruits [J]. Natural Product Sciences,2003,9 (2):112-116
[165]Tapiero H., Tew K.. D., Polyphenols:Do They Play a Role in the Prevention of Human Pathologies [J]. Biomedicine& Pharmacotherapy,2002,56 (4):200-207
[166]中华人民共和国国家标准[M].香料-旋光度的测定(GB／T 14454.5-2008),1-3
[167]中华人民共和国国家标准[M].植物油-碘值的测定(GB／T 5532-1995),255-258
[168]中华人民共和国国家标准[M].精油-酸值的测定(GB／T 14455.5-1993),278-279
[169]中华人民共和国国家标准[M].精油-酯值的测定(GB／T 14455.6-1993),280-281
[170]中华人民共和国国家标准[M].精油-乙醇中溶混度的评估(GB／T 14455.3-1993),272-275
[171]ACIS (Aroma Chemical Information Sevice), ESO-97 Database of Essential oils,1997
[172]Adams R. P., Identification of Essential Oil Components by Gas Chromatography/Mass Spectroscopy [M]. Allured Publishing Corporation, Carol Stream, IL, USA.2001:23-67
[173]Adams R. P., Identification of Essential Oil Components by Gas Chromatography/Mass Spectroscopy (4th ed) [M]. Allured Publishing Corporation, Carol Stream, IL, USA.2007:10-87
[174]Bisio A., Ciarallo G., Romussi G., et al. Chemical Composition of Essential Oils from Some Salvia species [J]. Phytotherapy Research,1998,12 (1):117-120
[175]丛浦珠.质谱学在天然有机化学中的应用[M].北京：科学出版社,1987：158-171
[176]丛浦珠,苏克曼.分析化学手册(第九分册,第二版)[M].北京：化学工业出版社,2000：247-276
[177]Kovats E., Gas Chromatographic Characterization of Organic Substances in the Retention Index System [J]. Advances in Chromatography,1965,1 (2):229-247
[178]Yu J. Q., Lei J. H., Yu H. D., et al. Chemical Composition and Antimicrobial Activity of the Essential Oil of Scutellaria barbata [J]. Phytochemistry,2004,65 (7):881-884
[179]Zhu S. Y., Yang Y, Yu H. D., et al. Chemical Composition and Antimicrobial Activity of the Essential Oils of Chrysanthemum indicum [J]. Journal of Ethnopharmacology,2005,96 (1-2):151-158
[180]马慕英.大蒜抗真菌作用研究[J].食品科学,1993,14(1)：7-11
[181]Tabanca N., Kirimer N., Demirci B., et al. Composition and Antimicrobial Activity of the Essential Oils of Micromeria cristata subsp. phyrgia and the Enantiomeric Distribution of Borneol [J]. Journal of Agricultural and Food Chemistry,2001,49 (9):4300-4303
[182]Tzakou O., Pitarokili D., Chinou I. B., et al. Composition and Antimicrobial Activity of the Essential Oil of Salvia ringens [J]. Planta Medica,2001,67 (1):81-83
[183]谭龙飞,钟彩莲,陈颖姬,等.咖哩粉精油的抗菌作用研究及GC-MS分析[J].中国调味品,2003,28(11)：6-10
[184]Juteau F., Masotti V., Bessiere J. M., et al. Antibacterial and Antioxidant Activities of Artemisia annua Essential Oil [J]. Fitoterapia,2002,73 (6):532-535
[185]夏忠弟,余俊龙.α-蒎烯对白色念珠菌生物合成的影响[J].中国现代医学杂志,2000,10(1)：44-46
[186]廖凤霞,辛龙涛,陈华,等.中药枳实与枳壳挥发油成分对比分析[J].重庆大学学报,2004,27(5)：38-40
[187]Sibanda S., Chigwada G, Poole M., et al. Composition and Bioactivity of the Leaf Essential Oil of Heteropyxis dehniae from Zimbabwe [J]. Journal of Ethnopharmacology,2004,92 (1):107-111
[188]Carson C. F., Riley T. V. Antimicrobial Activity of the Major Components of the Essential Oil of Melaleuca alternifolia [J]. Journal of Applied Bacteriology,1995,78 (3):264-269
[189]Cosentino S., Tuberoso C. I. G., Pisano B., et al. In-vitro Antimicrobial Activity and Chemical Composition of Sardinian thymus Essential Oils [J]. Letters in Applied Microbiology,1999,29 (2): 130-135
[190]王伟江.天然活性单萜—柠檬烯的研究进展[J].中国食品添加剂,2005,16(1)：33-37
[191]陈建伟,李祥,武露凌,等.中国珍稀植物明党参嫩茎叶挥发油化学成分研究[J].天然产物研究与开发,2000,12(3)：48-51
[192]危英,张旭,危莉,等.杏叶防风挥发油化学成分分析[J].贵阳中医学院学报,2005,27(4)：56-57
[193]Marino M., Bersani C., Comi G Impedance Measurements to Study the Antimicrobial Activity of Essential Oils from Lamiaceace and Compositae [J]. International Journal of Food Microbiology, 2001,67(3):187-195
[194]Kashiwada Y., Nonaka G., Nishioka I. Studies on Rhubarb (Rhei Rhizoma). VI. Isolation and Chara-cterization of Stilbenes [J]. Chemical& Pharmaceutical Bulletin,1984,32 (9):3501-3517
[195]Karl C., Muller G., Pedersen P. A. Flavonoideaus Salix alba. Die Strukturdes Terniflorins Undeines Weiteren Acylflavonoides [J]. Phytochemistry,1976,15 (6):1084-1085
[196]Aritomi M. Homoplantaginin, a New Flavonoid Glycoside in Leaves of Plantago asiatica Linnaeus [J]. Chemical& Pharmaceutical Bulletin,1967,15 (4):432-434
[197]Gonzalez A. G., Arteaga J. M., Breton J. L. et al. Five New Labdane Diterpene Oxides from Eupato rium jhanii [J]. Phytochemistry,1977,16 (1):107-110
[198]Ybarra M. I., Popich S., Borkosky S. A., et al. Manoyl Oxide Diterpenoids from Grindelia scorzoner ifolia [J]. Journal of Natural Products,2005,68 (4):554-558
[199]Ahmed A. A., Mahmoud A. A., Ahmed U. M., et al. Manoyl Oxide a-Arabinopyranoside and Grinde lic Acid Diterpenoids from Grindelia integrifolia [J]. Journal of Natural Products,2001,64 (10): 1365-1367
[200]Hamburger M., Hostettmann K. Hydroxycinnamic Acid Esters from Polygala chamaebuxus [J]. Phytochemistry,1985,24 (8):1793-1797
[201]Sakushima A., Coskun M., Tanker M., et al. A Sinapic Acid Ester from Boreava orientalis [J]. Phytochemistry,1994,35 (6):1481-1484
[202]Shan Y. P., Xu L. L., Lu Y., et al. Diterpenes from Coleus forskohlii (Willd.) Briq. (Labiatae) [J]. Chemical& Pharmaceutical Bulletin,2008,56 (1):52-56
[203]Yang X. D., Mei S. X., Zhao J. F., et al. A New Iridoid Glucoside from Lagotis yunnanensis [J]. Chinese Chemical Letters,2003,14 (9):936-938
[204]Xiao K., Xuan L. J, Xu Y. M, et al. Stilbene Glycoside Sulfates from Polygonum cuspidatum [J]. Journal of Natural Products,2000,63 (10):1373-1376
[205]Anjaneyulu A. S. R., Reddy A. V. R., Reddy D. S. K., et al. Pacharin:a New Dibenzo (2,3-6,7) Oxepin Derivative from Bauhinia racemosa Lamk [J]. Tetrahedron,1984,40 (21):4245-4252
[206]Shen Z. L., Zhuo G. L., Jiang X. Z., A Practical Synthesis of trans-Resveratrol [J]. Indian Journal of Chemistry (Section-B),2002,41 (11):2395-2398
[207]Wanjala C. C. W., Majinda R. R. T., A New Stilbene Glycoside from Elephantorrhiza goetzei [J]. Fitoterapia,2001,72 (6):649-655
[208]Gromova A. S., Tyukavkina N. A., Lutskii V. I., et al. Hydroxystilbenes of the Inner Bark of Pinus sibirica [J]. Chemistry of Natural Compounds,1975,11 (6):715-719
[209]Guo W. F., Sakata K., Watanabe N., et al. Geranyl 6-O-β-D-Xylopyranosyl-β-D-Glucopyranoside Isolated as an Aroma Precursor from Tea Leaves for Oolong Tea [J]. Phytochemistry,1993,33 (6): 1373-1375
[210]Kitagawa I., Sakagami M., Hashiuchi F., et al. Apioglycyrrhizin and Araboglycyrrhizin, Two New Sweet Oleanene-Type Triterpene Oligoglycosides from the Root of Glycyrrhiza Inflata [J]. Chemical & Pharmaceutical Bulletin,1989,37 (2):551-553
[211]Hara S., Okabe H., Mihashi K., Gas-Liquid Chromatographic Separation of Aldose Enantiomers as Trimethylsilyl Ethers of Methyl 2-(Polyhydroxyalkyl)-Thiazolidine-4(R)-Carboxylates [J]. Chemical & Pharmaceutical Bulletin,1987,35 (2):501-506
[212]Yang H., Sung S. H., Kim Y. C. Two New Hepatoprotective Stilbene Glycosides from Acer mono Leaves [J]. Journal of Natural Products,2005,68 (1):101-103
[213]Dorman D. E., Roberts J. D. Nuclear Magnetic Resonance Spectroscopy. Carbon-13 Spectra of Some Pentose and Hexose Aldopyranoses [J]. Journal of the American Chemical Society,1970,92 (5): 1355-1361
[214]Kamerling J. P., Bie M. J. A. D., Vliegenthart J. F. G. A PMR Study of the Anomeric Protons in Pertrimethylsilyl Oligosaccharides, a Determination of the Configuration of the Glycosidic Bond [J]. Tetrahedron,1972,28 (11):3037-3047
[215]Yamasaki K., Kasai R., Masaki Y. et al. Application of C-13 NMR to the Structural Elucidation of Acylated Plant Glycosides [J]. Tetrahedron Letters,1977,18 (14):1231-1234
[216]Agrawal P. K., Bansal M. C. Carbon-13 NMR of Flavonoids [M]. Amsterdam:Elsevier,1989:313-315
[217]Laphookhieo S., Karalai C., Ponglimanont C., et al. Pentacyclic Triterpenoid Esters from the Fruits of Bruguiera cylindrica [J]. Journal of Natural Products,2004,67 (5):886-888
[218]Nkengfack A. E., Vouffo T. W., Vardamides J. C., et al. Phenolic Metabolites from Eiythrina Species [J]. Phytochemistry,1997,46 (3):573-578
[219]杨学东,刘江云,许莉,等.蝉冀藤茎中蔗糖酯成分研究[J].高等学校化学学报,2003,24(1)：61-63
[220]Ioset J. R., Marston A., Gupta M. P., et al. Five New Prenylated Stilbenes from the Root Bark of Lonchocarpus chiricanus [J]. Journal of Natural Products,2001,64 (6):710-715
[221]Sridhar C., Subbaraju G. V., Venkateswarlu Y., et al. New Acylated Iridoid Glucosides from Vitex altissima [J]. Journal of Natural Products,2004,67 (12):2012-2016
[222]Chien N. Q., Hung N. V., Santarsiero B. D., et al. New 3-O-Acyl Betulinic Acids from Stiychnosvan prukii Craib [J]. Journal of Natural Products,2004,67 (6):994-998
[223]Zulfiqar A., Tetsuro I., Toshiyuki T., et al. Acetophenone C-Glucosides and Stilbene O-Glucosides in Upuna borneensis [J]. Phytochemistry,2004,65 (14):2141-2146
[224]Takahashi H., Hirata S., Minami H., et al. Triterpene and Flavahone Glycoside from Rhododendron simsii [J]. Phytochemistry,2001,56 (8):875-879
[225]Zou J. H., Yang J. S., Dong Y. S., et al. Flavone C-glycosides from Flowers of Trollius ledebouri [J]. Phytochemistry,2005,66 (10):1121-1125
[226]Zou J. H., Yang J. S., Zhou L. Acylated Flavone C-glycosides from Trollius ledebouri [J]. Journal of Natural Products,2004,67 (4):664-667
[227]尹建元,杨晓红,孟勤,等.刺楸中新七糖皂苷的结构解析[J].分析化学,2004,32(10)：1381-1384
[228]Mimakia Y., Fukushimaa M., Yokosukaa A., et al. Triterpene Glycosides from the Roots of Sanguisorba officinalis [J]. Phytochemistry,2001,57 (5):773-779
[229]姚新生.天然产物化学[M].北京：人民卫生出版社,1994：359-360
[230]Asada Y., Ueoka T., Furuyua T., Novel Acylated Saponins from Montbretia crocosmia(crocosmii flora). Isolation of Saponins and the Structures of Crocosmiosides A, B and H [J]. Chemical& Pharmaceutical Bulletin,1989,37 (8):2139-2146
[231]Zhao J., Nakamura N., Hattori M., et al. New Triterpenoid Saponins from the Roots of Sinocrassula asclepiadea [J]. Chemical& Pharmaceutical Bulletin,2004,52 (2):230-237
[232]路金才,徐菲菲,张新艳.两头尖的化学成分研究[J].药学学报,2002,37(9)：709-712
[233]Sang S. M., Lao A. N., Wang H. C., Two New Spirostanol Saponins from Allium tuberosum [J]. Journal of Natural Products,1999,62 (7):1028-1029
[234]Ishii H., Kitagawa I., Matsushita K., et al. The Configuration and Conformation of the Arabinose Moiety in Platycodins, Saponins Isolated from Platycodon grandiflorum, and Mi-saponins from Madhuca longifolia Based on Carbon-13 and Hydrogen-1 NMR Spectroscopic Evidence:The Total Structures of the Saponins [J]. Tetrahedron Letters,1981,22 (16):1529-1532
[235]Su Y. F., Koike K., Guo D., et al. New Apiose-Containing Triterpenoid Saponins from Conyza blinii [J]. Tetrahedron,2001,57 (31):6721-6726
[236]袁涛,华会明,裴月湖.三棱的化学成分研究(Ⅰ)[J].中草药,2005,36(11)：1607-1609
[237]Harrison L. J., Sia G L., Sim K. Y.5,7-Dihydroxy-8-Methoxyflavone from Tetracera indica [J]. Planta Medica,1994,60 (5):493-494
[238]王红燕,肖丽和,刘丽,等.粘毛黄芩根的化学成分研究[J].沈阳药科大学学报,2003,20(5)：339-341
[239]关玲,权丽辉,徐丽珍,等.广藿香化学成分的研究[J].中国中药杂志,1994,19(6)：356-358
[240]崔艳君,刘屏,陈若芸.鸡血藤有效成分研究[J].中国中药杂志,2005,30(2)：121-123
[241]Genichiro N. A New Class of Dimeric Flavan-3-ol Gallates, the Asinensins A and B, and Proantho-cyanidin Gallates from Green Tea Leaf [J]. Chemical& Pharmaceutical Bulletin,1983,31 (11): 3906-3914
[242]Hanke F. J., Kubo I., Naylor S. Assigning 13C-NMR Resonances of Natural Products [J]. Journal of Natural Products,1989,52(6):1237-1245
[243]Zheng Y. L., Bao J. C., Liu G., et al. Separation and Identification for 4-Hydroxy-3-Methoxy Benzaldehyde from Root of Panax quinquefolius L. [J].中国药学杂志,2006,41(7)：497-500
[244]Patra A., Ghosh G, Sengupta P. K., et al. Carbon-13 NMR Spectral Studies on Chalcones and Acetophenones [J]. Magnetic Resonance in Chemistry,1987,25 (4):734-736
[245]左国营,张志军,陈丽蓉,等.藏药黑蕊虎耳草的化学成分[J],云南植物研究,2005,27(6)：691-694
[246]丁岗,刘延泽,宋毛平,等.诃子中的多元酚类成分[J],中国药科大学学报,2001,32(3)：193-196
[247]Yan K., Lv J. L., Jia A., et al. Studies on Chemical Constituents of Minosa pudica [J]. Chinese Pharmaceutical Journal,2006,41 (16):1293-1295
[248]植飞,孔令义,彭司勋.大蓟化学成分的研究[J].药学学报,2003,38(6)：442-447
[249]Narui T., Sawada K., Takatsuki S., et al. NMR Assignments of Depsides and Tridepsides of the Lichen Family Umbilicariaceae [J]. Phytochemistry,1998,48 (5):815-822
[250]Fujimatu E., Ishikawa T., Kitajima J. Aromatic Compound Glucosides, Alkyl Glucoside and Glucide from the Fruit of Anise [J]. Phytochemistry,2003,63 (5):609-616
[251]黄胜阳,石建功,杨永春,等.藏药旺拉化学成分的研究[J].中国中药杂志,2002,27(2)：118 -120
[252]冯孝章,陈玉武,杨峻山.天麻化学成分的研究[J].化学学报,1979,37(3)：175-182
[253]徐俊平,董亲颐.仙茅化学成分的研究Ⅰ[J].中草药,1986,17(6)：8-10
[254]陈昌祥,倪伟,梅文莉.仙茅根茎中的配糖体[J].云南植物研究,1999,21(4)：521-524
[255]李宁,李铣,杨世林,等.过山蕨总黄酮的化学成分研究(Ⅰ)[J].沈阳药科大学学报,2004,21(2)：105-108
[256]Bernhard H., Josef H. New Chalcones from Bidens pilosa [J]. Planta Medica,1988,54 (1):52-54
[257]王建平,王名洲,高玉敏,等.鬼针草化学成分的研究(Ⅱ)[J].中草药,1995,26(1)：68-70
[258]Ishimaru K., Nishikawa K., Omoto T., et al. Two Flavone 2'-Glucosides from Scutellaria baicalensis [J]. Phytochemistry,1995,40 (1):279-281
[259]Yakushijin K., Tohshima T., Suzuki R., et al. Studies on the Constituents of the Plants of Illicium Species. II. Structures of Phenolic Components [J]. Chemical& Pharmaceutical Bulletin,1983,31 (8):2879-2823
[260]Mattivi F., Reniero F., Korhammer S. Isolation, Characterization, and Evolution in Red Wine Vinification of Resveratrol Monomers [J]. Journal of Agricultural and Food Chemistry,1995,43 (7): 1820-1823
[261]Shim H., Hong W., Ahn Y. Enzymatic Preparation of Phenolic Glucosides by Streptococcus Mutans [J]. Bulletin of the Korean Chemical Society,2003,24 (11):1680-1682
[262]Zhou X., Peng J. Y., Fan G. R., et al. Isolation and Purification of Flavonoid Glycosides from Trollius ledebouri using High-Speed Counter-Current Chromatography by Stepwise Increasing the Flow-Rate of the Mobile Phase [J]. Journal of Chromatography A,2005,1092 (2):216-221
[263]Soltis D. E., Bohm B. A. Flavonoids of Ascarina lucida [J]. Journal of Natural Products,1982,45 (4): 415-417
[264]Wagner H., Iyengar M. A., Beal J. L. Flavone C and O-glycoside in Thalictrum-arten [J]. Phytoche mistry,1971,10 (10):2553-2554
[265]康少文,于永芳,王沛.金莲花化学成分研究[J].中草药,1984,15(6)：7-9
[266]Kin Y. H., Chung B. S., Sankawa U. Pimaradiene Diterpenes from Acanthopanax koreanum [J]. Journal of Natural Products,1988,51 (6):1080-1083
[267]Chung B. S., Kim Y. Phytochemical Investigation of Acanthopanax koreanum, Proc. Inst. Sym. on New Drug Development from Natural Products [C]. Seoul:National University Press,1989:5-7
[268]刘向前,陆昌洙,张承烨.细柱五加皮化学成分的研究[J].中草药,2004,35(3)：250-252
[269]Klass J., Tinto W. F., Mclean S., et al. Friedel and Triterpenoids from Peritassa compta:Complete 1H and 13C Assignments by 2D NMR Spectroscopy [J]. Journal of Natural Products,1992,55 (11): 1626-1630
[270]Nawwar M. A. M., Buddrus J., Brauer H. Dimmer Phenolic Constituents from the Roots of Tamarix nilotica [J]. Phytochemistry,1982,21 (7):1755-1758
[271]Hiroki T., Sueo H., Sansei N. Coumarins from Bark of Fraxinus japonica and F. mandshurica var. japonica [J]. Chemical& Pharmaceutical Bulletin,1985,33 (9):4069-4073
[272]李占林,李铣,李宁,等.沙沂文冠果壳的化学成分[J].沈阳药科大学学报,2005,22(4)：271-273
[273]Takahira M., Kusano A., Shibano M., et al. Piscidic Acid and Fukiic Acid Esters from Cimicifuga simplex [J]. Phytochemistry,1998,49 (7):2115-2119
[274]Sang S., Lapsley K., Jeong W. S., et al. Antioxidative Phenolic Compounds Isolated from Almond Skins (Prunus Amygdalus Batsch) [J]. Journal of Agricultural and Food Chemistry,2002,50 (8): 2459-2463
[275]张雪,高昊,王乃利,等.金钗石斛中的酚性成分[J].中草药,2006,37(5)：652-655
[276]Paulo A., Jimeno M. L., Gomes E. T., et al. Steroidal Alkaloids from Cryptolepis obtusa [J]. Phytoch emistry,2000,53 (3):417-422
[277]李文武,丁立生,李伯刚.苎麻根化学成分的初步研究[J].中国中药杂志,1996,21(7)：427-428
[278]马天波,李孟广.毛白杨叶化学成分的研究[J].中草药,1987,18(3)：105-107
[279]Yano K., Sanada S., Ida Y., et al. Studies on the Constituents of the Bark of Kalopanax pictus Nakai [J]. Chemical& Pharmaceutical Bulletin,1991,39 (4):865-870
[280]Siddiqui S., Hafeez F., Begum S., et al. Oleanderol, A New Pentacyclic Triterpene from the Leaves of Nerium oleander [J]. Journal of Natural Products,1988,51 (2):229-233
[281]Cheminat A., Zawatzky R., Becker H., et al. Caffeoyl Conjugates from Echinacea Species:Structures and Biological Activity [J]. Phytochemistry,1988,27 (9):2787-2794
[282]Kusano A., Shibano M., Kusano G., et al. Studies on the Constituents of Cimicifuga Species XIX. Eight New Glycosides from Cimicifuga simplex Wormsk [J]. Journal of the Chemical& Pharmaceu-tical Bulletin,1996,44 (11):2078-2085
[283]中国科学院上海药物研究所,黄酮体化合物鉴定手册[M].北京：科学出版社,1981：394-620
[284]Markham K. R., Ternai B., Stanley R., et al. Carbon-13 NMR Studies of Flavonoide-III:Naturally Occuring Flavonoid Glycosides and Their Acylated Derivatives [J]. Tetrahedron,1978,34 (9): 1389-1397
[285]许宝安,舒晔,张明哲.金叶子黄酮类成分的研究[J].北京大学学报(自然科学版),1996,32(6)：699-702
[286]刘莹,惠玉虎.刺五加提取物中紫丁香甙、紫丁香树脂甙的HPLC测定法[J].中草药,2000,31(8)：587-588
[287]Gamal A. A. E, Takeya K., Irokawa H., et al. Lignan bis-Glucosides from Galium sinaicum [J]. Phyt-ochemistry,1997,45 (3):597-600
[288]Tung J. H., Lee C. O., Kim Y. C., et al. New Bioactive Cerebrosides from Arisaema amurense [J]. Journal of Natural Products,1996,59 (3):319-322
[289]Yook C. S., Chang S. Y., Lai J. H., Lupne-Glycoside of A. trifoliatus forma tristigmatis Leaves [J], Archives of Pharmacal Research,1999,22 (6):629-632
[290]Miyakoshi M., Shirasuna K., Hirai Y., et al. Triterpenoid Saponins of Acanthopanax nipponicus Leaves [J]. Journal of Natural Products,1999,62 (3):445-448
[291]Zou K., Zhao Y. Y., Tu G. Z., et al. Two Diastereomeric Saponins with Cytotoxic Activity from Albizia julibrissin [J]. Carbohydrate Research,2000,324 (3):182-188
[292]Tommasi N. D., Autore G, Bellino A., et al. Antiproliferative Triterpene Saponins from Trevesia palmata [J]. Journal of Natural Products,2000,63 (3):308-314
[293]Kasai R., Okihara M., Asakawa J., et al. 13C-NMR Study of a and β-Anomeric Pairs of D-mannopyr anosides and L-rhamnopyranosides [J]. Tetrahedron,1979,35 (11):1427-1432
[294]于德泉,杨俊山,周同惠,等.分析化学手册(第七分册,第二版)[M].北京：化学工业出版社,2002：796-798
[295]Kizu H., Shimana H., Tomimori T. Studies on the Constituents of Clematis Species. VI. The Consti-tuents of Clematis stans SIEB. et ZUCC. [J]. Chemical& Pharmaceutical Bulletin,1995,43 (12): 2187-2194
[296]Kang S. S., Kim J. S., Kim Y. H., et al. A Triterpenoid Saponin from Patrinia scabiosaefolia [J]. Journal of Natural Products,1997,60 (10):1060-1062
[297]Chen M., Wu W. W., Dkniel N., et al. Leonticins D-H, Five Triterpene Saponins from Leontice kiangnanensis [J]. Phytochemistry,1997,44 (3):497-504
[298]Shao C. J., Kasai R., Xu J. D., et al. Saponins from Leaves of Acanthopanax senticosus Harms, Ciwujia. II. Structures of Ciwujianosides A1, A2, A3, A4 and D3 [J]. Chemical& Pharmaceutical Bulletin,1989,37 (1):42-45
[299]黄儒强,郭子杰,邓伟玲,等.田七中总黄酮含量的测定及其黄酮种类的初步鉴别[J].现代食品科技,2007,23(3)：66-68
[300]陈孝泉.植物化学分类学[M].北京：高等教育出版社,1990：53-79
[301]谢鹏,张敏红.黄酮类化合物抑菌作用的研究进展[J].中国动物保健,2004,6(12)：35-37
[302]Wei H. C., Cai Q. Y, Rahn R. O., et al. Inhibition of UV Light and Fenton Reaction Induced Oxda-tive DNA Damage by the Soybean Isoflavone Genistein [J]. Carcinogenesis,1996,17 (1):73-78
[303]Masahiro K., Naoki I., Hiroichi N. Effects of Luteolin and Other Flavonoids on IgE-Mediated Allergic Reactions [J]. Planta Medica,2000,66 (1):25-29
[304]睢大赞,吕忠智,于晓凤.刺五加叶皂甙的抗实验性心律失常作用[J].中草药,1997,28(2)：99-101
[305]Francis G., Zohar K., Harinder P. S., et al. The Biological Action of Saponins in Animal Systems:A Review [J]. British Journal of Nutrition,2002,88 (6):587-605
[306]冯雪梅,叶红军,韩登春,等.刺五加叶皂甙对动物实验性肝癌抑制作用的研究[J].中国老年学杂志,1999,9(5)：298-299
[307]胡宗礼,黄晓萍.倒卵叶五加总皂苷对心肌缺血再灌注大鼠左心功能和血清一氧化氮及血浆内槲皮素的影响[J].中国现代应用药学,2003,20(6)：468-470
[308]陈小川,夏兰,胡松,等.短毛五加总皂苷在体外抑制人血小板聚集和血小板因子4释放[J].中国药理学报(英文),1996,17(6)：523-526
[309]Strickland F. M., Pelley R. P., Kripke M. L. Prevention of Ultraviolet Radiation-Induced Suppression of Contact and Delayed Hypersensitivity by Aloe barbadensis Gel Extract [J]. Journal of Investiga-tive Denmatology,1994,102(2):197-204
[310]Yagi A., Egusa T., Arase M. et al.. Isolation and Characterization of the Glycoprotein Fraction with a Proliferation-Promoting Activity on Human and Hamster Cells in vitro from Aloe vera Gel [J]. Planta Medica,1997,63 (1):18-21
[311]Hart L. A. T., Vanden Berg A. J. J., Kuis L. An Anti-Complementary Polysaccharide with Immunological Adjuvant Activity from the Leaf Parenchyma Gel of Aloe vera [J]. Planta Medica, 1989,55 (6):509-512
[312]Chen M. L., Song F. R., Guo M. Q., et al. Analysis of Flavonoid Constituents from Leaves of Acanthopanax senticosus Harms by Electrospray Tandem Mass Spectrometry [J]. Rapid Communi-cations in Mass Spectrometry,2002,16 (4):264-271
[313]Shi B.J., Nie X. H., Chen L. Z., et al. Anticancer Activities of a Chemically Sulfated Polysaccharide Obtained from Grifola frondosa and Its Combination with 5-Fluorouracil against Human Gastric Carcinoma Cells [J]. Carbohydrate Polymers,2007,68 (4):687-692
[314]刘芳,杨翠军,孙黎,等.五加皮多糖对人宫颈癌Hela细胞凋亡作用的研究[J],时珍国医国药,2009,20(5)：1178-1179
[315]刘国龙,毛海波,伍勇,等.红毛五加多糖对荷人喉癌裸鼠抗癌作用的研究[J],时珍国医国药,2008,19(8)：1964-1965
[316]Lee S. H., Lee Y. S., Jung S. H., et al. Antitumor and Immunostimulating Activities of Acanthopanax sessiliflorus Fruits [J]. Natural Product Sciences,2003,9 (2):112-116.
[317]唐兴文,董钰明,李春兰,等.红毛五加总苷和多糖药理作用研究进展[J].兰州医学院学报,2004,30(4)：93-96
[318]张惟杰.糖复合物生化研究技术[M].杭州：浙江大学出版社,1999：78-79
[319]韩公羽,沈企华.植物药有效成分的研究与开发[M].杭州：杭州大学出版社,1991：102-103