天麻复方降压胶囊的制备及其药效研究
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摘要
天麻复方降压胶囊处方来源于导师临床经验方,全方由天麻、菊花、枸杞三味药组成,具有滋阴潜阳、清肝明目、降低血压的功效。我们按照保健品的要求,根据处方中各味药的有效成分及其药效、药理等基础研究,应用现代提取、分离技术,提取有效部位群,结合先进成型工艺,将其制成胶囊剂型保健品。具体研究内容包括以下几个方面。
1.理论研究:以中医药理论为指导,阐述了本方的来源及立题依据和意义,同时对处方中各药材所含化学成分的理化性质等进行了归纳与总结。
2.制备工艺研究:以浸膏收率、天麻素含量和天麻多糖含量为指标,采用单因素实验和正交实验,优选了天麻药材的提取工艺;以浸膏收率和菊花总黄酮含量为指标,采用单因素实验和正交实验,优选了菊花药材的提取工艺;以浸膏收率和枸杞多糖含量为指标,采用单因素实验和正交实验,优选了枸杞药材的提取工艺。以药材提取物为原料,通过平行实验,优选了成型工艺中所需辅料的品种和配比,最后确定了制剂的成型工艺。
3.质量标准的初步研究:实现了对该产品原料药材和成品的质量标准的控制。采用薄层色谱法鉴别了制剂中的天麻、菊花,采用化学方法鉴别了制剂中的多糖成分;采用HPLC法测定了制剂中天麻素的含量。
4.药效学研究:选择100例原发性高血压患者,随机分为二组,其中40例予以西医常规治疗(对照组),60例在对照组治法的基础上,加用中药天麻复方降压胶囊(治疗组),分别进行临床药效观察。建立了小鼠的应激性高血压模型和肝阳上亢高血压模型,观察了天麻复方降压胶囊对高血压鼠的作用。
The prescription of Tianmafufangjiangya Capsule,which is composed of three Chinese herb medicine--Gastrodia elate Bl,Chrysanthemum morifolium Ramat and Lycium barbarum L is clinically curative for hypertension. According to the standards of healthy products,it is made into a capsule dosage form with the morden technology of extraction,purification and preparation after a basic study of pharmacochemistry,pharmacodynamics and pharmacology in the prescription.The study details are as follows.
1.Study on theories:With the guidance of traditional Chinese medicine theories,the source of the prescription,the survey of the studies and the significance of developing the issue are demonstrated and summarized widely.
2.Study on preparation process:With the index of the contents of extracta, gastrodin and polysaccharide,the extaction process of Gastrodia elate Bl was optimized by parallel-contrast and orthogonal experiments.With the index of the contents of extracta and total flavone,the extaction process of Chrysanthemum morifolium Ramat was optimized by parallel-contrast and orthogonal experiments.With the index of the contents of extracta and polysaccharide,the extraction process of Lycium barbarum L was optimized by parallel-contrast and orthogonal experiments.Taken the extraction as intermediate,purified process was optimized and the preparation process was established.
3.Study on quality control method:The quality standard of crude drugs and finished products was established.TLC method was taken for identification of Gastrodia elate Bl and Chrysanthemum morifolium Ramat.Chemical method was taken for identification of polysaccharides component.HPLC method was taken for determination of Gastrodine in Tianmafufangjiangya Capsules.
4.Study on pharmacodynamics:To divide 100 primary hypertension patients into two groups at random:control group,which is given conventional theraphies,including 40 patients and cure group,which is given both conventional therapies and the new product's therapies,including 60 patients and to do clinical research on the two groups.Irritability hypertension model and excessive rising of liver-YANG hypentension model of mouse were established and the product's function was evaluated and studied through the two models.
1.理论研究:以中医药理论为指导,阐述了本方的来源及立题依据和意义,同时对处方中各药材所含化学成分的理化性质等进行了归纳与总结。
2.制备工艺研究:以浸膏收率、天麻素含量和天麻多糖含量为指标,采用单因素实验和正交实验,优选了天麻药材的提取工艺;以浸膏收率和菊花总黄酮含量为指标,采用单因素实验和正交实验,优选了菊花药材的提取工艺;以浸膏收率和枸杞多糖含量为指标,采用单因素实验和正交实验,优选了枸杞药材的提取工艺。以药材提取物为原料,通过平行实验,优选了成型工艺中所需辅料的品种和配比,最后确定了制剂的成型工艺。
3.质量标准的初步研究:实现了对该产品原料药材和成品的质量标准的控制。采用薄层色谱法鉴别了制剂中的天麻、菊花,采用化学方法鉴别了制剂中的多糖成分;采用HPLC法测定了制剂中天麻素的含量。
4.药效学研究:选择100例原发性高血压患者,随机分为二组,其中40例予以西医常规治疗(对照组),60例在对照组治法的基础上,加用中药天麻复方降压胶囊(治疗组),分别进行临床药效观察。建立了小鼠的应激性高血压模型和肝阳上亢高血压模型,观察了天麻复方降压胶囊对高血压鼠的作用。
The prescription of Tianmafufangjiangya Capsule,which is composed of three Chinese herb medicine--Gastrodia elate Bl,Chrysanthemum morifolium Ramat and Lycium barbarum L is clinically curative for hypertension. According to the standards of healthy products,it is made into a capsule dosage form with the morden technology of extraction,purification and preparation after a basic study of pharmacochemistry,pharmacodynamics and pharmacology in the prescription.The study details are as follows.
1.Study on theories:With the guidance of traditional Chinese medicine theories,the source of the prescription,the survey of the studies and the significance of developing the issue are demonstrated and summarized widely.
2.Study on preparation process:With the index of the contents of extracta, gastrodin and polysaccharide,the extaction process of Gastrodia elate Bl was optimized by parallel-contrast and orthogonal experiments.With the index of the contents of extracta and total flavone,the extaction process of Chrysanthemum morifolium Ramat was optimized by parallel-contrast and orthogonal experiments.With the index of the contents of extracta and polysaccharide,the extraction process of Lycium barbarum L was optimized by parallel-contrast and orthogonal experiments.Taken the extraction as intermediate,purified process was optimized and the preparation process was established.
3.Study on quality control method:The quality standard of crude drugs and finished products was established.TLC method was taken for identification of Gastrodia elate Bl and Chrysanthemum morifolium Ramat.Chemical method was taken for identification of polysaccharides component.HPLC method was taken for determination of Gastrodine in Tianmafufangjiangya Capsules.
4.Study on pharmacodynamics:To divide 100 primary hypertension patients into two groups at random:control group,which is given conventional theraphies,including 40 patients and cure group,which is given both conventional therapies and the new product's therapies,including 60 patients and to do clinical research on the two groups.Irritability hypertension model and excessive rising of liver-YANG hypentension model of mouse were established and the product's function was evaluated and studied through the two models.
引文
[1]何晶.天麻素的药理作用及临床应用[J].天津药学,2006,18(5):62-63
[2]胡一冰,崔佳,韩笑等.中药天麻研究进展[J].贵阳中医学院学报,2001,23(4):48-51
[3]尚伟芬.天麻药理作用研究进展[J].中草药,1997,28(10):629-632
[4]Hajjar I,Kotchen TA.Trends in prevalence,awarness,treatment and control of hypertension in the United States[J].JAMA,2003,290:199-206
[5]Lund-Johansen P.Current issues in the control of hypertension[J].J Hum Hypertens,1995,9(suppl 5):S25-S28
[6]黄哲生.人口老化与老年血压病[J].中国民族学院学报(自然科学版),1996,01:76-78
[7]孙凤,郭淑霞.我国高血压流行情况及其社区防治进展[J].农垦医学,2005,27(6):460-463
[8]Rao M S.Pathogenesis and consequences of essential hypertension[J],J Indian Med Assoc,2003,100(4):251
[9]张江蓉.交感神经与高血压关系的研究现状[J].中国心血管杂志,1999,4(3):181-183
[10]黄震华.抗高血压药物与血管内皮功能[J].中国新药与临床杂志,2005,24(5):398-401
[11]王先梅,杨丽霞.原发性高血压发病机制的研究进展[J].西南国防医药,2005,15(1):98-100
[12]Ruppert V,Maisch B.Genetics of hunman hypertension[J].Herz,2003,28(8):625-626
[13]Weinberger M H.Salt sensitivity:does it play an important role in pathogenesis and treatment of hypertension[J].Curr Opin in Nephro and Hypertens,1996,5(1):206-208
[14]魏志泉.人参皂苷单体Rb1对抗高血压发展过程研究[D].东北师范大学硕士学位论文,2004
[15]程轶群,李晓辉.若干实验性高血压大鼠模型的介绍[J].中国比较医学杂志,2006,16(5):305-308
[16]范平,司军强,刘政江等.自发性高血压大鼠模型的应用研究进展[J].农垦医学,2004,26(6):444-448
[17]Mullins JJ,Peters J,,Ganten D.Fulminant hypertension in transgenic rats harbouring the mouse Ren-2 gene[J].Nature,1990,344:541-544
[18]徐叔云,卞如濂,陈修.药理实验方法学[M].北京:人民卫生出版社,1991:818-819
[19]王秀卿,高贞,郭红等.慢性应激性高血压动物模型的建立[J].白求恩军医学院学报,2003,1(4):202-203
[20]王钦茂,李莉,赵勇等.清化胶囊对三种因素复合制造大鼠高血压肝阳上亢证模型的治疗作用[J].中国实验方剂学杂志,2002,8(3):14-17
[21]Takahashi Nobuyuki,Smithies Oliver.Human genetics,animal models and computer simulations for studying hypertension[J].Trends in Genetics,2004,20(3):136-145
[22]Juan G Abraldes,Marcos Pasarin,Juan Carlos Garcia-Pagan.Animal models of portal hypertension[J].World Journal of Gastroenterology,2006,12(41):6577-6584
[23]张丽娜.灵菇菌乳Ⅰ号、Ⅱ号对高血压模型大鼠的降压作用的实验研究[D].东北林业大学硕士学位论文,2004
[24]任朝晖,赵富强.现代血压测量[J].医疗设备信息,2001,1:32-34
[25]何铁春.四肢血压同步测量仪研制原理与医学实践[J].中国生物医学工程学报,2002,21(2):182-186
[26]K.Yanakoshi et al.Indirect Measurement of instantaneous Arterial Blood Pressure in the Human Finger by the Vasular Unloading Technique[J].IEEE Transa on Biom Eng Comput,1979,17(1):11-47
[27]柳兆荣,李惜惜.血液动力学原理与方法[M].上海:复旦大学出版社,1997:168-196
[28]焦学军,房兴业.利用脉搏波特征参数连续测量血压的方法研究[J].生物医学工程学杂志,2002,19(2):217-220
[29]王涤松.三种实验性高血压模型大鼠血压波动性研究[D].第二军医大学博士学位论文,2002
[30]Majahalms,Turjanmaa V,Weder AB,Vusitalo A.Evaluation of antihypertensive therapy after 5-year follow-up[J].Hypertension,1996,28(5):725
[31]Goa KL,Wagstaff AJ.A review of antihypertensive drug[J].Grugs,1996,51:820
[32]段小军.高血压肝阳上亢证大鼠模型的复制及天麻钩藤饮的干预作用研究[D].广州中医药大学硕士学位论文,2006
[33]张崇泉,刘柏炎.中医药治疗高血压病临床研究进展[J].湖南中医杂志,1996,12(4):51-52
[34]蔡传家,解连庆.天麻钩藤饮治疗原发性高血压病人60例临床研究[J].中国医药情报,1999,5(1):8-10
[35]邓祥雄,黄荣璋.天麻钩藤饮加减治疗高血压病63例[J].吉林中医药,2002,22(3):10
[36]陈康远.六味地黄汤加味治疗原发性高血压377例疗效观察[J].新中医,2003,35(5):41-42
[37]魏霞,穆广海.高血压病辩证治体会[J].中国医药学报,2004,19(1):59
[38]蔡永敏.中国药名辞典(第1版)[M].北京:中国中医药出版社,1996:47
[39]张宏杰,周建军,李新生.天麻研究进展[J].氨基酸和生物资源,2003,15(1):17-20
[40]沈卫华,顾雪中.天麻素紫外分光光度法测定量探讨[J].时珍国医国药,1999,9:666
[41]杨更生,王德先,杨云霞,等.天麻及其伪品的毛细管电泳鉴别及天麻素的测定[J].中草药,2001,32(4):351-352
[42]Linda Moretti Ojemann,Wendel L.Nelson,Donella S.Shin,Ann Ojemann Rowe,Robert A.Buchanan.Tian ma,an ancient Chinese herb,offers new options for the treatment of epilepsy and other conditions[J].Epilepsy & Behavior,2006,8:376-383
[43]杨世海,尹春梅,王秀全.天麻的研究进展[J].人参研究,1995,4:11-14
[44]Eun-Kyoung Ahn,HYe-Jin Jeon,Eun-Ju Lim,et al.Anti-inflammatory and anti-angiogenic activities of Gastrodia elata Blume[J].Journal of Ethnopharmacology,2007,110:476-482.
[45]Hyeon-Ju Kim,Kwang-Deog Moon,Dong-Seok Lee,Sang-Han Lee.Ethyl ether fraction of Gastrodia Elata Blume protects amyloid β peptide-induced cell death[J].Journal of Ethnopharmacology,2003,84:95-98.
[46]朱彩平.枸杞多糖的结构分析及生物活性评价[D].华中农业大学博士学位论文,2006.
[47]刘茵.枸杞子的化学成分及药理研究新进展[A].第六次全国中西医结合实验医学学术研讨会会议论文集[C],2002
[48]整意端,赵邦爱.枸杞肽A和B用于治疗高血压[J].国外医药·植物药分册,1992,7(5):235-236
[49]Yi-Chun Yeh,Tae-Shik Hahm,CrisCina M.Sabliov,Y.Martin Lo.Effects of Chinese wolfberry(Lycium Chinese P.Mill.) leaf hydrolysates on the growth of Pediococcus acidilactici[J].Bioresource Technology,2008(99):1383-1393
[50]耿长山,王葛英,林永栋等.枸杞子多糖对老年小鼠免疫功能低下的调节作用[J].中华老年医学杂志,1989,8(4):236-239
[51]胡国俊,白惠卿,杜守英等.枸杞对TB淋巴细胞增值和T细胞亚群变化的调节作用[J].中国免疫学杂志,1995,11(3):163-165
[52]赵长琦,章灵华,钱玉昆.抗肿瘤植物药及其有效成分[M].中国中医药出版社,1997,5:140-141
[53]张熙,谢先春.枸杞多糖抗氧化作用的实验研究[J].中国中药杂志,1993,18(2):110-112
[54]贾月霞,时安云.枸杞多糖对肾性高血压大鼠血管活性物质的影响[J].北京医科大学学报,1997,29(5);429-432
[55]罗琼,李谨伟,张声华.枸杞多糖-X组分对糖尿病家兔降血糖的效果[J].营养学报,1997,19(2):173-177
[56]罗琼,阎俊,李谨伟,张声华.枸杞多糖粗品与纯品抗疲劳作用的比较[J].营养学报,1999,21(3):310-317
[57]国家中医药管理局《中华本草》编委会.中华本草(第7卷)[M].上海:上海科学技术出版社,1999:806-809
[58]王硕.菊花化学品质的比较及杭白菊提取物制备工艺的研究[D].沈阳药科大学硕士学位论文,2005
[59]矢原正治,森田宁,野原念红.菊花的成分研究[J].生物学杂志,1990,44(4):335
[60]刘金旗,沈其权,刘劲松等.贡菊化学成分的研究[J].中国中药杂志,2001,26(8):547-548
[61]胡立宏,陈仲良.杭白菊的化学成分研究:正戊基果糖苷的结构测定[J].植物学报,1997,39(2):181-184
[62]李英霞,彭广芳,王小梅等.菊花的药理研究进展[J].时珍国医国药,1998,9(6):580
[63]方家选,臧文华.怀菊花浸膏对自发性高血压大鼠血压及其靶器官脂质过氧化作用的影响[J].河南中医学院学报,2006,21(126):18-19
[64]方志峰,黄林,李庆华等.三七菊花茶冲剂辅助治疗高血压效果观察[J].广西预防医学,2004,10(3):174-175
[2]胡一冰,崔佳,韩笑等.中药天麻研究进展[J].贵阳中医学院学报,2001,23(4):48-51
[3]尚伟芬.天麻药理作用研究进展[J].中草药,1997,28(10):629-632
[4]Hajjar I,Kotchen TA.Trends in prevalence,awarness,treatment and control of hypertension in the United States[J].JAMA,2003,290:199-206
[5]Lund-Johansen P.Current issues in the control of hypertension[J].J Hum Hypertens,1995,9(suppl 5):S25-S28
[6]黄哲生.人口老化与老年血压病[J].中国民族学院学报(自然科学版),1996,01:76-78
[7]孙凤,郭淑霞.我国高血压流行情况及其社区防治进展[J].农垦医学,2005,27(6):460-463
[8]Rao M S.Pathogenesis and consequences of essential hypertension[J],J Indian Med Assoc,2003,100(4):251
[9]张江蓉.交感神经与高血压关系的研究现状[J].中国心血管杂志,1999,4(3):181-183
[10]黄震华.抗高血压药物与血管内皮功能[J].中国新药与临床杂志,2005,24(5):398-401
[11]王先梅,杨丽霞.原发性高血压发病机制的研究进展[J].西南国防医药,2005,15(1):98-100
[12]Ruppert V,Maisch B.Genetics of hunman hypertension[J].Herz,2003,28(8):625-626
[13]Weinberger M H.Salt sensitivity:does it play an important role in pathogenesis and treatment of hypertension[J].Curr Opin in Nephro and Hypertens,1996,5(1):206-208
[14]魏志泉.人参皂苷单体Rb1对抗高血压发展过程研究[D].东北师范大学硕士学位论文,2004
[15]程轶群,李晓辉.若干实验性高血压大鼠模型的介绍[J].中国比较医学杂志,2006,16(5):305-308
[16]范平,司军强,刘政江等.自发性高血压大鼠模型的应用研究进展[J].农垦医学,2004,26(6):444-448
[17]Mullins JJ,Peters J,,Ganten D.Fulminant hypertension in transgenic rats harbouring the mouse Ren-2 gene[J].Nature,1990,344:541-544
[18]徐叔云,卞如濂,陈修.药理实验方法学[M].北京:人民卫生出版社,1991:818-819
[19]王秀卿,高贞,郭红等.慢性应激性高血压动物模型的建立[J].白求恩军医学院学报,2003,1(4):202-203
[20]王钦茂,李莉,赵勇等.清化胶囊对三种因素复合制造大鼠高血压肝阳上亢证模型的治疗作用[J].中国实验方剂学杂志,2002,8(3):14-17
[21]Takahashi Nobuyuki,Smithies Oliver.Human genetics,animal models and computer simulations for studying hypertension[J].Trends in Genetics,2004,20(3):136-145
[22]Juan G Abraldes,Marcos Pasarin,Juan Carlos Garcia-Pagan.Animal models of portal hypertension[J].World Journal of Gastroenterology,2006,12(41):6577-6584
[23]张丽娜.灵菇菌乳Ⅰ号、Ⅱ号对高血压模型大鼠的降压作用的实验研究[D].东北林业大学硕士学位论文,2004
[24]任朝晖,赵富强.现代血压测量[J].医疗设备信息,2001,1:32-34
[25]何铁春.四肢血压同步测量仪研制原理与医学实践[J].中国生物医学工程学报,2002,21(2):182-186
[26]K.Yanakoshi et al.Indirect Measurement of instantaneous Arterial Blood Pressure in the Human Finger by the Vasular Unloading Technique[J].IEEE Transa on Biom Eng Comput,1979,17(1):11-47
[27]柳兆荣,李惜惜.血液动力学原理与方法[M].上海:复旦大学出版社,1997:168-196
[28]焦学军,房兴业.利用脉搏波特征参数连续测量血压的方法研究[J].生物医学工程学杂志,2002,19(2):217-220
[29]王涤松.三种实验性高血压模型大鼠血压波动性研究[D].第二军医大学博士学位论文,2002
[30]Majahalms,Turjanmaa V,Weder AB,Vusitalo A.Evaluation of antihypertensive therapy after 5-year follow-up[J].Hypertension,1996,28(5):725
[31]Goa KL,Wagstaff AJ.A review of antihypertensive drug[J].Grugs,1996,51:820
[32]段小军.高血压肝阳上亢证大鼠模型的复制及天麻钩藤饮的干预作用研究[D].广州中医药大学硕士学位论文,2006
[33]张崇泉,刘柏炎.中医药治疗高血压病临床研究进展[J].湖南中医杂志,1996,12(4):51-52
[34]蔡传家,解连庆.天麻钩藤饮治疗原发性高血压病人60例临床研究[J].中国医药情报,1999,5(1):8-10
[35]邓祥雄,黄荣璋.天麻钩藤饮加减治疗高血压病63例[J].吉林中医药,2002,22(3):10
[36]陈康远.六味地黄汤加味治疗原发性高血压377例疗效观察[J].新中医,2003,35(5):41-42
[37]魏霞,穆广海.高血压病辩证治体会[J].中国医药学报,2004,19(1):59
[38]蔡永敏.中国药名辞典(第1版)[M].北京:中国中医药出版社,1996:47
[39]张宏杰,周建军,李新生.天麻研究进展[J].氨基酸和生物资源,2003,15(1):17-20
[40]沈卫华,顾雪中.天麻素紫外分光光度法测定量探讨[J].时珍国医国药,1999,9:666
[41]杨更生,王德先,杨云霞,等.天麻及其伪品的毛细管电泳鉴别及天麻素的测定[J].中草药,2001,32(4):351-352
[42]Linda Moretti Ojemann,Wendel L.Nelson,Donella S.Shin,Ann Ojemann Rowe,Robert A.Buchanan.Tian ma,an ancient Chinese herb,offers new options for the treatment of epilepsy and other conditions[J].Epilepsy & Behavior,2006,8:376-383
[43]杨世海,尹春梅,王秀全.天麻的研究进展[J].人参研究,1995,4:11-14
[44]Eun-Kyoung Ahn,HYe-Jin Jeon,Eun-Ju Lim,et al.Anti-inflammatory and anti-angiogenic activities of Gastrodia elata Blume[J].Journal of Ethnopharmacology,2007,110:476-482.
[45]Hyeon-Ju Kim,Kwang-Deog Moon,Dong-Seok Lee,Sang-Han Lee.Ethyl ether fraction of Gastrodia Elata Blume protects amyloid β peptide-induced cell death[J].Journal of Ethnopharmacology,2003,84:95-98.
[46]朱彩平.枸杞多糖的结构分析及生物活性评价[D].华中农业大学博士学位论文,2006.
[47]刘茵.枸杞子的化学成分及药理研究新进展[A].第六次全国中西医结合实验医学学术研讨会会议论文集[C],2002
[48]整意端,赵邦爱.枸杞肽A和B用于治疗高血压[J].国外医药·植物药分册,1992,7(5):235-236
[49]Yi-Chun Yeh,Tae-Shik Hahm,CrisCina M.Sabliov,Y.Martin Lo.Effects of Chinese wolfberry(Lycium Chinese P.Mill.) leaf hydrolysates on the growth of Pediococcus acidilactici[J].Bioresource Technology,2008(99):1383-1393
[50]耿长山,王葛英,林永栋等.枸杞子多糖对老年小鼠免疫功能低下的调节作用[J].中华老年医学杂志,1989,8(4):236-239
[51]胡国俊,白惠卿,杜守英等.枸杞对TB淋巴细胞增值和T细胞亚群变化的调节作用[J].中国免疫学杂志,1995,11(3):163-165
[52]赵长琦,章灵华,钱玉昆.抗肿瘤植物药及其有效成分[M].中国中医药出版社,1997,5:140-141
[53]张熙,谢先春.枸杞多糖抗氧化作用的实验研究[J].中国中药杂志,1993,18(2):110-112
[54]贾月霞,时安云.枸杞多糖对肾性高血压大鼠血管活性物质的影响[J].北京医科大学学报,1997,29(5);429-432
[55]罗琼,李谨伟,张声华.枸杞多糖-X组分对糖尿病家兔降血糖的效果[J].营养学报,1997,19(2):173-177
[56]罗琼,阎俊,李谨伟,张声华.枸杞多糖粗品与纯品抗疲劳作用的比较[J].营养学报,1999,21(3):310-317
[57]国家中医药管理局《中华本草》编委会.中华本草(第7卷)[M].上海:上海科学技术出版社,1999:806-809
[58]王硕.菊花化学品质的比较及杭白菊提取物制备工艺的研究[D].沈阳药科大学硕士学位论文,2005
[59]矢原正治,森田宁,野原念红.菊花的成分研究[J].生物学杂志,1990,44(4):335
[60]刘金旗,沈其权,刘劲松等.贡菊化学成分的研究[J].中国中药杂志,2001,26(8):547-548
[61]胡立宏,陈仲良.杭白菊的化学成分研究:正戊基果糖苷的结构测定[J].植物学报,1997,39(2):181-184
[62]李英霞,彭广芳,王小梅等.菊花的药理研究进展[J].时珍国医国药,1998,9(6):580
[63]方家选,臧文华.怀菊花浸膏对自发性高血压大鼠血压及其靶器官脂质过氧化作用的影响[J].河南中医学院学报,2006,21(126):18-19
[64]方志峰,黄林,李庆华等.三七菊花茶冲剂辅助治疗高血压效果观察[J].广西预防医学,2004,10(3):174-175