FATP1和FATP4基因在浙东白鹅和朗德鹅中的发育性表达研究
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摘要
目前,很多的学者研究发现了在长链脂肪酸摄取中一些候选蛋白如脂肪酸转运蛋白(FATP),脂肪酸合成蛋白(FABP),长链脂肪酰基辅酶A合成酶(ACSL)和脂肪酸易位酶(FAT/CD36)对长链脂肪酸的摄取和代谢具有重要作用,其中FATP对脂质代谢和脂肪相关疾病有着重要的影响,为此,FATPs基因家族的研究成为了当前的热点。
本研究以浙东白鹅和朗德鹅为试验材料,采用SYBR green I定量PCR技术,以β-actin为内参基因,分析了浙东白鹅和朗德鹅在1d、14d、21d、28d、35d、42d、49d、56d、63d等这9个时间点FATP1和FATP4基因表达的组织差异,以及在胸肌、腿肌、心脏、肝脏、肠、腹脂、皮脂等组织中FATP1和FATP4基因表达量的发育性变化。结果表明：浙东白鹅和朗德鹅胸肌中FATP1基因的表达在35d均达到表达最高峰,在49d进入表达最低谷：腿肌中浙东白鹅FATP1基因的表达量在63d达到了最大,而朗德鹅是在35d达到了最大;皮脂中浙东白鹅在21d出现表达最高峰,而朗德鹅在14d出现最高峰,之后就急剧下降;腹脂中浙东白鹅在42d出现表达最高峰,而朗德鹅35d出现第一个峰也是最高峰；两个品种均在56d进入表达量最低谷；肝脏中浙东白鹅和朗德鹅分别在1d和28d时达到了最大：心脏中浙东白鹅FATP1基因的表达量在56d达到了最大,而朗德鹅是在63d达到了最大。
结果还表明,浙东白鹅和朗德鹅胸肌中FATP4基因的表达分别在63d和35d达到表达最高峰；腿肌中浙东白鹅和朗德鹅两个品种均在56d达到表达最高峰：皮脂中浙东白鹅在21d出现表达最高峰,而朗德鹅在14d出现最高峰,之后就急剧下降;腹脂中浙东白鹅在35d出现表达最高峰,而朗德鹅除了14d不同以外,其他时间点的表达量较为平稳;肝脏中浙东白鹅和朗德鹅分别在56d和14d时达到了最大；心脏中浙东白鹅FATP4基因的表达量在56d达到了最大,而朗德鹅是在63d达到了最大。FATP1基因和FATP4基因在两个鹅品种和不同组织不同时间点均有表达,组织表达丰度不一样,其中腿肌是两个基因的优势表达组织,表达量显著高于其他各组织(P<0.05)。
FATP1基因在两个品种间的表达差异是在腿肌和胸肌差异显著(P<0.05),而其他组织差异不显著(P>0.05)；FATP4基因在两个品种间的表达差异不显著(P>0.05)。通过对FATP1基因与FATP4基因表达量的倍比关系分析得出,FATP1基因的表达量高于FATP4基因的表达量。
本研究首次发现,FATP1基因在浙东白鹅和朗德鹅小肠中均有微量表达。由此,在FATPs家族中FATP4基因不再是唯一在小肠表达的成员。
At present, several candidate proteins have been proposed to be involved in the uptake process of LCFAs including fatty acid transport proteins (FATPs),fatty acid binding protein (FABPs), long-chain fatty acyl-CoA synthetases (ACSL) and fatty acid translocase (FAT/CD36).Although many of the above-mentioned proteins have important role in the proceeding of LCFAs uptake and metabolism, recent in vivo studies have particularly highlighted the contribution of FATPs to lipid metabolism and fatty acid-associated disorders. So,the studies about FATP are being focused in today because of FATP special character and biological function.
In the studies, we adopted East White Geese and Landes Geese as materials. We used the real-time PCR (SYBR green I) method to test the expression quantity of FATP1 and FATP4 gene in different tissues and the expression quantity at different growth points.The results showed that:the expression quantity of FATPl gene in breast muscle reached the highest expression in 35 day,and reached the lowest expression in 49 day in the two breeds;the expression quantity of FATP1 gene in leg muscle reached the highest expression in Zhedong White Geese in 63 day,and reached the highest expression in Landes Geese in 35 day; the expression quantity of FATP1 gene in Sebum reached the highest expression in 21 day in Zhedong White Geese,and reached the highest expression in Landes Geese in 14 day,and after sharply;the expression quantity of FATP1 gene in abdominal fat reached the highest expression in Zhedong White Geese in 42 day,and reached the highest expression in Landes Geese in 35 day; the expression quantity of FATP1 gene in liver reached the highest expression respectively in Zhedong White Geese and Landes Geese in 1 and 28 day; the expression quantity of FATP1 gene in heart reached the highest expression in Zhedong White Geese in 56 day,and reached the highest expression in Landes Geese in 63 day.
The results also show that the expression quantity of FATP4 gene in breast muscle reached the highest expression respectively in Zhedong White Geese and Landes Geese in 63 and 35 day; the expression quantity of FATP4 gene in leg muscle reached the highest expression in 56 day in the two breeds; the expression quantity of FATP4 gene in Sebum reached the highest expression in 21 day in Zhedong White Geese,and reached the highest expression in Landes Geese in 14 day,and after sharply; the expression quantity of FATP4 gene in abdominal fat reached the highest expression in Zhedong White Geese in 35 day,and the expression of Landes Geese except 14 day the other times points is relatively stable quantity of expression; the expression quantity of FATP4 gene in liver reached the highest expression respectively in Zhedong White Geese and Landes Geese in 56 and 14day; the expression quantity of FATP4 gene in heart reached the highest expression in Zhedong White Geese in 56 day,and reached the highest expression in Landes Geese in 63 day. the expression quantity of FATP1 and FATP4 had many expressions between the two breeds and at different growth points and tissues.Moreover,the expression of abundance have difference. The expression of advantage tissue is leg muscle between the two breeds and the expression is significantly higher than other organizations (P<0.05).
The results showed that the expression quantity of FATP1 had significant difference between the two breeds in leg muscle and breast muscle, the difference of expression is significantly(P<0.05),and the other tissues had no significant differences(P>0.05);the expression quantity of FATPA had no significant difference between the two breeds(P>0.05). The expression of FATP1 gene is higher than FATP4 gene.
In the studies,we found that the expression quantity of FATP1 gene had small trace expression in intestine.This is belong to first discovered in Landes geese and Zhedong White geese. Thus, the expression of FATP4 gene in the small intestine is not the only members in FATPs family.
本研究以浙东白鹅和朗德鹅为试验材料,采用SYBR green I定量PCR技术,以β-actin为内参基因,分析了浙东白鹅和朗德鹅在1d、14d、21d、28d、35d、42d、49d、56d、63d等这9个时间点FATP1和FATP4基因表达的组织差异,以及在胸肌、腿肌、心脏、肝脏、肠、腹脂、皮脂等组织中FATP1和FATP4基因表达量的发育性变化。结果表明：浙东白鹅和朗德鹅胸肌中FATP1基因的表达在35d均达到表达最高峰,在49d进入表达最低谷：腿肌中浙东白鹅FATP1基因的表达量在63d达到了最大,而朗德鹅是在35d达到了最大;皮脂中浙东白鹅在21d出现表达最高峰,而朗德鹅在14d出现最高峰,之后就急剧下降;腹脂中浙东白鹅在42d出现表达最高峰,而朗德鹅35d出现第一个峰也是最高峰；两个品种均在56d进入表达量最低谷；肝脏中浙东白鹅和朗德鹅分别在1d和28d时达到了最大：心脏中浙东白鹅FATP1基因的表达量在56d达到了最大,而朗德鹅是在63d达到了最大。
结果还表明,浙东白鹅和朗德鹅胸肌中FATP4基因的表达分别在63d和35d达到表达最高峰；腿肌中浙东白鹅和朗德鹅两个品种均在56d达到表达最高峰：皮脂中浙东白鹅在21d出现表达最高峰,而朗德鹅在14d出现最高峰,之后就急剧下降;腹脂中浙东白鹅在35d出现表达最高峰,而朗德鹅除了14d不同以外,其他时间点的表达量较为平稳;肝脏中浙东白鹅和朗德鹅分别在56d和14d时达到了最大；心脏中浙东白鹅FATP4基因的表达量在56d达到了最大,而朗德鹅是在63d达到了最大。FATP1基因和FATP4基因在两个鹅品种和不同组织不同时间点均有表达,组织表达丰度不一样,其中腿肌是两个基因的优势表达组织,表达量显著高于其他各组织(P<0.05)。
FATP1基因在两个品种间的表达差异是在腿肌和胸肌差异显著(P<0.05),而其他组织差异不显著(P>0.05)；FATP4基因在两个品种间的表达差异不显著(P>0.05)。通过对FATP1基因与FATP4基因表达量的倍比关系分析得出,FATP1基因的表达量高于FATP4基因的表达量。
本研究首次发现,FATP1基因在浙东白鹅和朗德鹅小肠中均有微量表达。由此,在FATPs家族中FATP4基因不再是唯一在小肠表达的成员。
At present, several candidate proteins have been proposed to be involved in the uptake process of LCFAs including fatty acid transport proteins (FATPs),fatty acid binding protein (FABPs), long-chain fatty acyl-CoA synthetases (ACSL) and fatty acid translocase (FAT/CD36).Although many of the above-mentioned proteins have important role in the proceeding of LCFAs uptake and metabolism, recent in vivo studies have particularly highlighted the contribution of FATPs to lipid metabolism and fatty acid-associated disorders. So,the studies about FATP are being focused in today because of FATP special character and biological function.
In the studies, we adopted East White Geese and Landes Geese as materials. We used the real-time PCR (SYBR green I) method to test the expression quantity of FATP1 and FATP4 gene in different tissues and the expression quantity at different growth points.The results showed that:the expression quantity of FATPl gene in breast muscle reached the highest expression in 35 day,and reached the lowest expression in 49 day in the two breeds;the expression quantity of FATP1 gene in leg muscle reached the highest expression in Zhedong White Geese in 63 day,and reached the highest expression in Landes Geese in 35 day; the expression quantity of FATP1 gene in Sebum reached the highest expression in 21 day in Zhedong White Geese,and reached the highest expression in Landes Geese in 14 day,and after sharply;the expression quantity of FATP1 gene in abdominal fat reached the highest expression in Zhedong White Geese in 42 day,and reached the highest expression in Landes Geese in 35 day; the expression quantity of FATP1 gene in liver reached the highest expression respectively in Zhedong White Geese and Landes Geese in 1 and 28 day; the expression quantity of FATP1 gene in heart reached the highest expression in Zhedong White Geese in 56 day,and reached the highest expression in Landes Geese in 63 day.
The results also show that the expression quantity of FATP4 gene in breast muscle reached the highest expression respectively in Zhedong White Geese and Landes Geese in 63 and 35 day; the expression quantity of FATP4 gene in leg muscle reached the highest expression in 56 day in the two breeds; the expression quantity of FATP4 gene in Sebum reached the highest expression in 21 day in Zhedong White Geese,and reached the highest expression in Landes Geese in 14 day,and after sharply; the expression quantity of FATP4 gene in abdominal fat reached the highest expression in Zhedong White Geese in 35 day,and the expression of Landes Geese except 14 day the other times points is relatively stable quantity of expression; the expression quantity of FATP4 gene in liver reached the highest expression respectively in Zhedong White Geese and Landes Geese in 56 and 14day; the expression quantity of FATP4 gene in heart reached the highest expression in Zhedong White Geese in 56 day,and reached the highest expression in Landes Geese in 63 day. the expression quantity of FATP1 and FATP4 had many expressions between the two breeds and at different growth points and tissues.Moreover,the expression of abundance have difference. The expression of advantage tissue is leg muscle between the two breeds and the expression is significantly higher than other organizations (P<0.05).
The results showed that the expression quantity of FATP1 had significant difference between the two breeds in leg muscle and breast muscle, the difference of expression is significantly(P<0.05),and the other tissues had no significant differences(P>0.05);the expression quantity of FATPA had no significant difference between the two breeds(P>0.05). The expression of FATP1 gene is higher than FATP4 gene.
In the studies,we found that the expression quantity of FATP1 gene had small trace expression in intestine.This is belong to first discovered in Landes geese and Zhedong White geese. Thus, the expression of FATP4 gene in the small intestine is not the only members in FATPs family.
引文
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