桂皮紫萁（Osmunda cinnamomea L. var. asiatica）成熟与萌发孢子差异表达蛋白质组学研究
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摘要
桂皮紫萁(也称分株紫萁)为紫萁蕨科紫萁蕨属的多年生草本植物,具食用、药用价值。其孢子萌发过程是其完成有性生殖发育与生活史周期的重要生物学事件。目前,对孢子萌发分子机制的认识并不清楚。
本研究比较了桂皮紫萁成熟孢子与萌发孢子的细胞学特征,及其差异表达蛋白质组特征。我们在蛋白质双向电泳凝胶上分别获得了桂皮紫萁成熟孢子与萌发孢子中表达的1255个和1375个蛋白质斑点。其中大多数蛋白质斑点都分布在pH5-6.5范围内,且多数蛋白质的分子量都分布在30-80kD之间。我们利用图像分析软件获得了成熟孢子与萌发孢子差异表达的101个蛋白质斑点(代表85种蛋白质)。在101个差异蛋白质斑点中,有37个表达丰度上调,64个表达丰度下调。这些差异表达蛋白质分别归属于以下9个功能类群:(1)光合作用、(2)糖类和能量代谢、(3)转录与翻译调控相关、(4)膜定位和转运、(5)蛋白质代谢、(6)氨基酸代谢、(7)细胞骨架动态变化、(8)信号转导和(9)未知功能。其中RuBisCo大亚基、磷酸核酮糖激酶、细胞色素f、景天庚酮糖-1,7-二磷酸酶、S-腺苷-L-高半胱氨酸水解酶、HSP90、谷氨酰胺合成酶等蛋白质分别参与光合作用、转录调控、蛋白质折叠、防御胁迫等重要生理过程。
这些成熟孢子与萌发孢子差异表达蛋白质组学特征为深入分析孢子萌发的分子机制提供了蛋白质组学证据,为桂皮紫萁的快速繁殖提供了理论依据。
Osmunda cinnamomea L.var.asiatica is a kind of edible and medicinal herbaceous perennid in the genus Osmunda in Osmundaceae,whose spore germination is an important biology event for the sexual reproduction growth and life history cycle.At present,the molecule mechanism of spore germination is not well-known.
We described the cytological features of mature and germinated O.cinnamomea spores, and compared the changes of proteome between them in this research.We obtained 1255 and 1375 protein spots on the 2DE gel from mature and germinated spores,respectively.Most of the protein spots in the two samples were distributed in pH5-6.5,and the majority of them were distributed in 30-80kD.By using the image analyse software,we obtained 101 differentially-expressed protein spots(represented 85 unique proteins) between mature and germinated spores.Of the 101 differentially-expressed protein spots,37 were up-regulated and 64 were down-regulated.These proteins can be assigned into 9 function categories respectively:(1) photosynthesis,(2)carbohydrate and energy metabolism,(3)transcriptional regulation-related, (4)membrane and transport,(5)protein metabolism,(6)amino acid metabolism,(7)cell structure, (8)signal transduction,(9)unknown proteins.Of them,the RuBisCo big subunit, phosphoribulokinase,cytochrome f,sedoheptulose 1,7-bisphosphate,S-adenosyl-L-homocysteine hydrolase,HSP90 and lutamine synthetase take part in photosynthesis, transcription regulation,protein folding and stress response respectively.
These differently expressed protemics characteristics between mature and germinated spores provided protemics evidence for the further research of spore germination molecule mechanism,and also provided theoretical basis for the rapid reproduction of O.cinnamomea.
本研究比较了桂皮紫萁成熟孢子与萌发孢子的细胞学特征,及其差异表达蛋白质组特征。我们在蛋白质双向电泳凝胶上分别获得了桂皮紫萁成熟孢子与萌发孢子中表达的1255个和1375个蛋白质斑点。其中大多数蛋白质斑点都分布在pH5-6.5范围内,且多数蛋白质的分子量都分布在30-80kD之间。我们利用图像分析软件获得了成熟孢子与萌发孢子差异表达的101个蛋白质斑点(代表85种蛋白质)。在101个差异蛋白质斑点中,有37个表达丰度上调,64个表达丰度下调。这些差异表达蛋白质分别归属于以下9个功能类群:(1)光合作用、(2)糖类和能量代谢、(3)转录与翻译调控相关、(4)膜定位和转运、(5)蛋白质代谢、(6)氨基酸代谢、(7)细胞骨架动态变化、(8)信号转导和(9)未知功能。其中RuBisCo大亚基、磷酸核酮糖激酶、细胞色素f、景天庚酮糖-1,7-二磷酸酶、S-腺苷-L-高半胱氨酸水解酶、HSP90、谷氨酰胺合成酶等蛋白质分别参与光合作用、转录调控、蛋白质折叠、防御胁迫等重要生理过程。
这些成熟孢子与萌发孢子差异表达蛋白质组学特征为深入分析孢子萌发的分子机制提供了蛋白质组学证据,为桂皮紫萁的快速繁殖提供了理论依据。
Osmunda cinnamomea L.var.asiatica is a kind of edible and medicinal herbaceous perennid in the genus Osmunda in Osmundaceae,whose spore germination is an important biology event for the sexual reproduction growth and life history cycle.At present,the molecule mechanism of spore germination is not well-known.
We described the cytological features of mature and germinated O.cinnamomea spores, and compared the changes of proteome between them in this research.We obtained 1255 and 1375 protein spots on the 2DE gel from mature and germinated spores,respectively.Most of the protein spots in the two samples were distributed in pH5-6.5,and the majority of them were distributed in 30-80kD.By using the image analyse software,we obtained 101 differentially-expressed protein spots(represented 85 unique proteins) between mature and germinated spores.Of the 101 differentially-expressed protein spots,37 were up-regulated and 64 were down-regulated.These proteins can be assigned into 9 function categories respectively:(1) photosynthesis,(2)carbohydrate and energy metabolism,(3)transcriptional regulation-related, (4)membrane and transport,(5)protein metabolism,(6)amino acid metabolism,(7)cell structure, (8)signal transduction,(9)unknown proteins.Of them,the RuBisCo big subunit, phosphoribulokinase,cytochrome f,sedoheptulose 1,7-bisphosphate,S-adenosyl-L-homocysteine hydrolase,HSP90 and lutamine synthetase take part in photosynthesis, transcription regulation,protein folding and stress response respectively.
These differently expressed protemics characteristics between mature and germinated spores provided protemics evidence for the further research of spore germination molecule mechanism,and also provided theoretical basis for the rapid reproduction of O.cinnamomea.
引文
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