家蚕（Bombyx mori）耐氟性研究和细胞色素P450基因cyp6u1及cyp6α8的分子克隆与表达分析
|
摘要
氟中毒是严重危害人体健康的主要地方病之一,该病危害严重,不仅能够造成氟斑牙、氟骨症等硬组织的广泛损伤,还可引起全身软组织不同程度的广泛损害。阐明氟中毒致病机制,是当今研究的热点问题之一。
细胞色素P450酶系也就是多功能氧化酶(MFO),是所有需氧生物体都存在的代谢酶系。细胞色素P450基因由35亿年前的一个共同祖先进化而成,是最古老和最庞大的超基因家族之一。它对代谢与转化内源物质和活化与降解外源化合物进行催化和调控。昆虫中的细胞色素P450酶系作用涉及生长、发育、取食等过程,其对异生物质的代谢特性导致了昆虫对杀虫剂的抗药性和对有毒物质的耐受性。
家蚕(Bombyx mori是鳞翅目昆虫的模式生物,鳞翅目是昆虫中的第二大目。本研究基于家蚕基因组精细图和EST、全基因组基因芯片数据,对家蚕细胞色素P450基因Bmcyp6ul和Bmcyp6a8基因进行了克隆和生物信息学研究。本论文的主要研究结果如下：
1.家蚕耐氟性研究分析
研究了家蚕添氟方法及桑叶氟浓度检测方法。桑叶用200ug/mLNaF浸渍15分钟,自然晾干。将桑叶研磨,加入1mol/L的HCl浸泡1小时(轻微震荡),pH5-6的TISAB缓冲液定容至50ml,氟离子选择电极检测氟浓度,能够精细地检测出桑叶的氟浓度值。
筛选了家蚕耐氟性品种T6和敏感性品种734,检测出耐氟阈值200ug/mL。用200 ug/mL的NaF浸渍桑叶15min,4龄起蚕开始饲喂家蚕,发现耐氟性品种T6虽然体重增长速率低于清水对照组,但是仍然能够一直稳步成长至上蔟：而同时同等条件喂养的734却体重增长异常缓慢,至4龄后期,渐渐死亡,最终不能进入眠期,全部死亡。
检测了NaF对家蚕食桑量的影响。相对于对照组,T6和734的添氟组食桑量都有所减少,但是734食桑量减少量异常,严重影响了正常生长；检测了NaF对家蚕蚕沙量的影响。统计显示,添氟组蚕沙量明显低于对照组。除了食桑量减少造成的蚕沙量减少,NaF造成家蚕排泄困难。
检测了氟中毒家蚕体内氟含量和各组织的氟含量。统计显示耐氟品种T6和敏感品种734的蚕沙氟含量一直上升；敏感品种734排氟量在到达36ug后就直线下降,一直在36ug徘徊；T6排氟量到达233ug后开始下降。分析显示氟排泄是家蚕耐氟性的一种重要途径。检测了氟中毒家蚕T6各组织的氟含量,包括头、血液、表皮,中肠、马氏管、丝腺、精巢(卵巢),发现头部组织和马氏管中氟离子含量最高。头部组织对氟化钠的不敏感性与氟代谢相配合是家蚕耐氟性另一重要途径。检测了氟中毒家蚕蛾期和蚕卵的氟含量,发现蛾的氟含量约等于5龄7天的氟含量。
检测了家蚕添氟组与对照组的乙酰胆碱酯酶含量和活性变化。结果显示,与对照组相比,添氟组头部酶原蛋白含量明显升高,乙酰胆碱酯酶活性呈规律性的明显降低。分析显示,家蚕乙酰胆碱酯酶是家蚕耐氟性的重要靶标之一。乙酰胆碱酯酶酶原蛋白量的增加弥补了活性的缺失,可能是耐氟品种头部组织对氟不敏感性的原因。
2.家蚕细胞色素P450第六家族基因的克隆及功能研究
采用生物信息学方法获得与果蝇cyp6ul基因同源的家蚕cyp6ul基因序列,实验结果表明,该基因ORF为1476 bp,编码491个氨基酸,推定的蛋白质分子质量为56.15 kD,等电点为9.23。(GenBank登录号：HM130560)。同源性分析表明：Bmcyp6ul基因与蜜蜂同源基因cyp6AS13的相似性为56%：与拟南芥cyp72A82的相似性为48%；与人的cyp3A7基因的相似性为50%。芯片数据分析表明,Bmcyp6ul基因在家蚕5龄第3天幼虫组织表达量很低,只在精巢组织稍有表达,推测该基因具有功能特异性。依据家蚕基因组数据,通过BLASTP比对,RT-PCR克隆了家蚕Bmcyp6a8基因。结果表明,该基因ORF为1572bp,编码523个氨基酸,推定的蛋白质分子质量为61.52kD,等电点为8.17,只有1个内含子。生物信息学分析显示,该基因位于第16号染色体,与野蚕cyp6AE8的相似性较高,同源性为93%,与棉铃虫的同源性为57%,与人的cyp3A43的同源性为33%。GenBank登录号为：GQ241737。从NCBI上下载人、果蝇、蜜蜂、斑马鱼、线虫的基因组与Bmcyp6a8进行比对,发现同源性基因全部是P450基因,构建进化树,Bmcyp6a8与蜜蜂第六家族基因聚集成簇。芯片表达图谱显示,Bmcyp6a8基因具有功能特异性。
Fluorosis is a serious harm to human health. It is one of the main endemic disease and it has serious threat. It can not only cause dental fluorosis, skeletal fluorosis hard tissue disorders such extensive damage, but also the body can cause varying degrees of extensive soft tissue damage. Clarify the pathogenesis of fluorosis is one of the hot issues of today's research.
Cytochrome P450 enzymes are also said to be mixed function oxidases, they are complex family of heme-containing enzymes,and they are found in all aerobic organisms. Cytochrome P450 is one of the oldest and largest gene superfamilies. It is evolved from a common ancestor who has existed for more than 3.5 billion years. Cytochrome P450s have Many functions including the metabolism of both endogenous substrates and xenobiotics. P450 enzymes metabolize insecticides, poisonous substance in bioactivation or in detoxification, and the latter process being enhanced in many strains with resistence to insecticides. Furthermore, P450 metabolism of certain chemicals is often the key to the adaptation of insect herbivores.
Silkworm(Bombyx mori) is the model organism for Lepidoptera, the second biggest order in insects. On basis of the completing genome sequence database of silkworm, coupled with its ESTs and the whole genome microarray. We analyzed the sequence structure and studied on the cloning and function of orthologous Bmcyp6ul and Bmcyp6a8. The main results present as follows.
1. Silkworm study of fluoride-resistant
We studied how mulberry silkworm add fluoride concentration and methods of fluoride detection. Mulberry soak with 200 ug/mL NaF for 15 minutes, air dry. The leaf grinding, adding 1mol/L of Hcl soak 1 hour (mild concussion), pH5-6 of TISAB buffer volume to 50ml, fluoride concentration of fluoride ion selective electrode detection, can detect the actual leaf fluoride concentrations.
Screen of silkworm of fluoride-resistant strains T6 and sensitive strains 734. It is detected that the threshold of resistant fluoride T6 is 200 ug/mL. Mulberry leaves treated with 200ug/mL NaF of for 25min was feeding to the larvae at the first day of forth instar of silkworms. Fluoride-resistant strains were found although the weight rising rate is less than clean water control group, but still be able to collect a steady growth in first; while simultaneously feeding the 734 the same conditions but it is unusually slow weight rising, and to the late 4th instar, gradually to die, eventually can not molt, and all died.
Examined the amount of mulberry leaves treated with NaF of the larvaes. Compared with the control group, T6, and 734 of the amount of mulberry leaves treated with fluoride had decrease the amount of mulberry leaves. But the larvae of 734 reduced too fast to grow normallyl; Tested NaF on the amount of litterof Silkworm. Statistics show that 734F excrement was significantly lower than other groups. NaF caused difficulties on silkworm excretion.
Examined the fluoride concentration of fluorosis silkworm body and its organizations.734F discarded the highest amount of F from 48h to 72h, valued 36ug. F content did not increase in litter, though 734F was fed with the same F treated leaves everyday. F concentration of silkworm litter in T6F kept increasing till the 7th in fifth star. Analysis showed that Fluoride excretion of the silkworm is an important fluoride-resistant pathway. The F concentration in the tissues of the fluorosis larvae was measured respectively in order to see how strain T6 resists F. It was found that the F concentration was the highest in the malpighian tubule and its value was 103.5ug/mL. The next was in head which F concentration value was 43.3ug/mL. We detected the head, the malpighian tubule, the hemocyte, the fat body, the integument, the midgut, the ovary and the silk gland, but F were highly found in the head and the malpighian tubule. Head organization is not sensitive sodium fluoride is another important pathway of resistant fluoride as well as fluoride metabolism in silkworm. Detection fluoride concentration of fluorosis silkworm moth eggs, the fluoride concentration is found that the fluoride concentration of silkworm moth eggs is equal to moth and the 7th day of fifth instar of larvae approximatively.
Detection of the protain content and acetylcholinesterase activity of the larvae treated with fluoride and the control groups. The results showed that compared with the control group, the protein content in head treated with fluoride is significantly higher. and while acetylcholinesterase activity was significantly lower regularity. Analysis showed that silkworm AChE content and activity is one of the important target.of silkworm resistance to fluoride. AChE protein increases make up for the lack of activity maybe causes the head organization of fluoride-resistant strains insensitivity to fluoride.
2. Bmcyp6ul cloning of silkworm and function of gene
The sequence homology with Drosophila cyp6ul gene of silkworm was obtained by bioinformatics.Its ORF is 1476bp, encoding 491 amino acids. It is deduced that its molecular weight is 56.15 kD and isoelectric point is 9.23. The cDNA of the testis of silkworm larvae third day in fifth instar as a template, using PCR primers designed to amplify, a treaty of 1500 bp bands was obtained, the size of the ORF sequence is close to silkworm cyp6ul predictive value, and named Bmcyp6ul. (GenBank Accession "Number:HM130560). Homologous analysis showed that it is more closely related to cyp6AS13 of Apis mellifera (56%), cyp72A82 of Arabidopsis thaliana (48%) and cyp3A7 of Homo sapiens (50%). Microarray data analysis shows that Bmcyp6ul gene in larvae of third day in 5th instar has only low levels of expression in the testis. It suggests the gene functional specificity.
3. Bmcyp6a8 cloning of Silkworm and function of gene
Based on the bioinformation of the genomic sequences database of Bombyx mori, a new gene was successf ully cloned from the head mRNA of B. mori by RT-PCR. Its ORF (open reading frame) includes a segment 1572 bp in length and encodes 523 amino acid residues, with the deduced molecular weight of 61.52 kD and isoelectric point of 8.17. Alignment of the cDNA with the silkworm genomic sequences revealed that there is only one 1706bp intron. It is designated Bmcyp6a8 (GenBank accession number:GQ241737). Compare with the other Paralogous gene of cytochrome P450 subfamily 6 in silkworm, only the Bmcyp6a8 has gene expression of the posterior silk gland. It suggests the gene functional specificity.
细胞色素P450酶系也就是多功能氧化酶(MFO),是所有需氧生物体都存在的代谢酶系。细胞色素P450基因由35亿年前的一个共同祖先进化而成,是最古老和最庞大的超基因家族之一。它对代谢与转化内源物质和活化与降解外源化合物进行催化和调控。昆虫中的细胞色素P450酶系作用涉及生长、发育、取食等过程,其对异生物质的代谢特性导致了昆虫对杀虫剂的抗药性和对有毒物质的耐受性。
家蚕(Bombyx mori是鳞翅目昆虫的模式生物,鳞翅目是昆虫中的第二大目。本研究基于家蚕基因组精细图和EST、全基因组基因芯片数据,对家蚕细胞色素P450基因Bmcyp6ul和Bmcyp6a8基因进行了克隆和生物信息学研究。本论文的主要研究结果如下：
1.家蚕耐氟性研究分析
研究了家蚕添氟方法及桑叶氟浓度检测方法。桑叶用200ug/mLNaF浸渍15分钟,自然晾干。将桑叶研磨,加入1mol/L的HCl浸泡1小时(轻微震荡),pH5-6的TISAB缓冲液定容至50ml,氟离子选择电极检测氟浓度,能够精细地检测出桑叶的氟浓度值。
筛选了家蚕耐氟性品种T6和敏感性品种734,检测出耐氟阈值200ug/mL。用200 ug/mL的NaF浸渍桑叶15min,4龄起蚕开始饲喂家蚕,发现耐氟性品种T6虽然体重增长速率低于清水对照组,但是仍然能够一直稳步成长至上蔟：而同时同等条件喂养的734却体重增长异常缓慢,至4龄后期,渐渐死亡,最终不能进入眠期,全部死亡。
检测了NaF对家蚕食桑量的影响。相对于对照组,T6和734的添氟组食桑量都有所减少,但是734食桑量减少量异常,严重影响了正常生长；检测了NaF对家蚕蚕沙量的影响。统计显示,添氟组蚕沙量明显低于对照组。除了食桑量减少造成的蚕沙量减少,NaF造成家蚕排泄困难。
检测了氟中毒家蚕体内氟含量和各组织的氟含量。统计显示耐氟品种T6和敏感品种734的蚕沙氟含量一直上升；敏感品种734排氟量在到达36ug后就直线下降,一直在36ug徘徊；T6排氟量到达233ug后开始下降。分析显示氟排泄是家蚕耐氟性的一种重要途径。检测了氟中毒家蚕T6各组织的氟含量,包括头、血液、表皮,中肠、马氏管、丝腺、精巢(卵巢),发现头部组织和马氏管中氟离子含量最高。头部组织对氟化钠的不敏感性与氟代谢相配合是家蚕耐氟性另一重要途径。检测了氟中毒家蚕蛾期和蚕卵的氟含量,发现蛾的氟含量约等于5龄7天的氟含量。
检测了家蚕添氟组与对照组的乙酰胆碱酯酶含量和活性变化。结果显示,与对照组相比,添氟组头部酶原蛋白含量明显升高,乙酰胆碱酯酶活性呈规律性的明显降低。分析显示,家蚕乙酰胆碱酯酶是家蚕耐氟性的重要靶标之一。乙酰胆碱酯酶酶原蛋白量的增加弥补了活性的缺失,可能是耐氟品种头部组织对氟不敏感性的原因。
2.家蚕细胞色素P450第六家族基因的克隆及功能研究
采用生物信息学方法获得与果蝇cyp6ul基因同源的家蚕cyp6ul基因序列,实验结果表明,该基因ORF为1476 bp,编码491个氨基酸,推定的蛋白质分子质量为56.15 kD,等电点为9.23。(GenBank登录号：HM130560)。同源性分析表明：Bmcyp6ul基因与蜜蜂同源基因cyp6AS13的相似性为56%：与拟南芥cyp72A82的相似性为48%；与人的cyp3A7基因的相似性为50%。芯片数据分析表明,Bmcyp6ul基因在家蚕5龄第3天幼虫组织表达量很低,只在精巢组织稍有表达,推测该基因具有功能特异性。依据家蚕基因组数据,通过BLASTP比对,RT-PCR克隆了家蚕Bmcyp6a8基因。结果表明,该基因ORF为1572bp,编码523个氨基酸,推定的蛋白质分子质量为61.52kD,等电点为8.17,只有1个内含子。生物信息学分析显示,该基因位于第16号染色体,与野蚕cyp6AE8的相似性较高,同源性为93%,与棉铃虫的同源性为57%,与人的cyp3A43的同源性为33%。GenBank登录号为：GQ241737。从NCBI上下载人、果蝇、蜜蜂、斑马鱼、线虫的基因组与Bmcyp6a8进行比对,发现同源性基因全部是P450基因,构建进化树,Bmcyp6a8与蜜蜂第六家族基因聚集成簇。芯片表达图谱显示,Bmcyp6a8基因具有功能特异性。
Fluorosis is a serious harm to human health. It is one of the main endemic disease and it has serious threat. It can not only cause dental fluorosis, skeletal fluorosis hard tissue disorders such extensive damage, but also the body can cause varying degrees of extensive soft tissue damage. Clarify the pathogenesis of fluorosis is one of the hot issues of today's research.
Cytochrome P450 enzymes are also said to be mixed function oxidases, they are complex family of heme-containing enzymes,and they are found in all aerobic organisms. Cytochrome P450 is one of the oldest and largest gene superfamilies. It is evolved from a common ancestor who has existed for more than 3.5 billion years. Cytochrome P450s have Many functions including the metabolism of both endogenous substrates and xenobiotics. P450 enzymes metabolize insecticides, poisonous substance in bioactivation or in detoxification, and the latter process being enhanced in many strains with resistence to insecticides. Furthermore, P450 metabolism of certain chemicals is often the key to the adaptation of insect herbivores.
Silkworm(Bombyx mori) is the model organism for Lepidoptera, the second biggest order in insects. On basis of the completing genome sequence database of silkworm, coupled with its ESTs and the whole genome microarray. We analyzed the sequence structure and studied on the cloning and function of orthologous Bmcyp6ul and Bmcyp6a8. The main results present as follows.
1. Silkworm study of fluoride-resistant
We studied how mulberry silkworm add fluoride concentration and methods of fluoride detection. Mulberry soak with 200 ug/mL NaF for 15 minutes, air dry. The leaf grinding, adding 1mol/L of Hcl soak 1 hour (mild concussion), pH5-6 of TISAB buffer volume to 50ml, fluoride concentration of fluoride ion selective electrode detection, can detect the actual leaf fluoride concentrations.
Screen of silkworm of fluoride-resistant strains T6 and sensitive strains 734. It is detected that the threshold of resistant fluoride T6 is 200 ug/mL. Mulberry leaves treated with 200ug/mL NaF of for 25min was feeding to the larvae at the first day of forth instar of silkworms. Fluoride-resistant strains were found although the weight rising rate is less than clean water control group, but still be able to collect a steady growth in first; while simultaneously feeding the 734 the same conditions but it is unusually slow weight rising, and to the late 4th instar, gradually to die, eventually can not molt, and all died.
Examined the amount of mulberry leaves treated with NaF of the larvaes. Compared with the control group, T6, and 734 of the amount of mulberry leaves treated with fluoride had decrease the amount of mulberry leaves. But the larvae of 734 reduced too fast to grow normallyl; Tested NaF on the amount of litterof Silkworm. Statistics show that 734F excrement was significantly lower than other groups. NaF caused difficulties on silkworm excretion.
Examined the fluoride concentration of fluorosis silkworm body and its organizations.734F discarded the highest amount of F from 48h to 72h, valued 36ug. F content did not increase in litter, though 734F was fed with the same F treated leaves everyday. F concentration of silkworm litter in T6F kept increasing till the 7th in fifth star. Analysis showed that Fluoride excretion of the silkworm is an important fluoride-resistant pathway. The F concentration in the tissues of the fluorosis larvae was measured respectively in order to see how strain T6 resists F. It was found that the F concentration was the highest in the malpighian tubule and its value was 103.5ug/mL. The next was in head which F concentration value was 43.3ug/mL. We detected the head, the malpighian tubule, the hemocyte, the fat body, the integument, the midgut, the ovary and the silk gland, but F were highly found in the head and the malpighian tubule. Head organization is not sensitive sodium fluoride is another important pathway of resistant fluoride as well as fluoride metabolism in silkworm. Detection fluoride concentration of fluorosis silkworm moth eggs, the fluoride concentration is found that the fluoride concentration of silkworm moth eggs is equal to moth and the 7th day of fifth instar of larvae approximatively.
Detection of the protain content and acetylcholinesterase activity of the larvae treated with fluoride and the control groups. The results showed that compared with the control group, the protein content in head treated with fluoride is significantly higher. and while acetylcholinesterase activity was significantly lower regularity. Analysis showed that silkworm AChE content and activity is one of the important target.of silkworm resistance to fluoride. AChE protein increases make up for the lack of activity maybe causes the head organization of fluoride-resistant strains insensitivity to fluoride.
2. Bmcyp6ul cloning of silkworm and function of gene
The sequence homology with Drosophila cyp6ul gene of silkworm was obtained by bioinformatics.Its ORF is 1476bp, encoding 491 amino acids. It is deduced that its molecular weight is 56.15 kD and isoelectric point is 9.23. The cDNA of the testis of silkworm larvae third day in fifth instar as a template, using PCR primers designed to amplify, a treaty of 1500 bp bands was obtained, the size of the ORF sequence is close to silkworm cyp6ul predictive value, and named Bmcyp6ul. (GenBank Accession "Number:HM130560). Homologous analysis showed that it is more closely related to cyp6AS13 of Apis mellifera (56%), cyp72A82 of Arabidopsis thaliana (48%) and cyp3A7 of Homo sapiens (50%). Microarray data analysis shows that Bmcyp6ul gene in larvae of third day in 5th instar has only low levels of expression in the testis. It suggests the gene functional specificity.
3. Bmcyp6a8 cloning of Silkworm and function of gene
Based on the bioinformation of the genomic sequences database of Bombyx mori, a new gene was successf ully cloned from the head mRNA of B. mori by RT-PCR. Its ORF (open reading frame) includes a segment 1572 bp in length and encodes 523 amino acid residues, with the deduced molecular weight of 61.52 kD and isoelectric point of 8.17. Alignment of the cDNA with the silkworm genomic sequences revealed that there is only one 1706bp intron. It is designated Bmcyp6a8 (GenBank accession number:GQ241737). Compare with the other Paralogous gene of cytochrome P450 subfamily 6 in silkworm, only the Bmcyp6a8 has gene expression of the posterior silk gland. It suggests the gene functional specificity.
引文
1. Greenwood N N,Earnshaw A.元素化学(中册)[M].北京：化学工业出版社,1996.
2. 陈冠荣主编.化工百科全书第4卷,发光材料一氟[M].北京：化学工业出版社.1993.
3.王娟韩薇.天然矿泉水中微量元素与人体健康[J].微量元素与健康研究.2001,18(2)：77-78.
4.侯振江周秀艳.微量元素与疾病[J].微量元素与健康研究.2004,21(6)：16-17.
5.孙殿军.中国地方病病情与防治进展[J].疾病控制杂志.2002,6(2)：97-100.
6. Ravichandran B, Roychowdhury A, Mukerjee A K, Gangopadhyay H N, Saiyed. Estimation of Fluoride Content in the Edible Vegetables of an Industrial Area in Orissa:Asian Journal of Water, Environment and Pollution,2007;2:93-95.
7. Guan ZZ, Xiao KQ, Zeng XY, Long YG, Cheng YH, Jiang SF, Wang YN.Changed cellular membrane lipid composition and lipid peroxidation of kidney in rats with chronic fluorosis: Arch Toxicol.2000,74:602-60.
8. World Health Organization (WHO). International Standards for Drinking Water. Geneva,2nd ed. 1963.
9. Jankovic S. In Manuel de chimie de l'environnement:OMS, Geneva 1974:251.
10. Hichour M, Persin F, Sandeaux J, Claude Gavach.Fluoride removal from waters by Donnan dialysis:Separation and Purification Technology.2000,18:1-11.
11. Mohapatra M, Anand S, Mishra BK, Giles Dion E, Singh P. Review of fluoride removal from drinking water:Journal of Environmental Management.2009:91:1-11.
12. Karthikeyan G, Shanmugasundarraj A. Isopleth mapping and In-Situ fluoride dependence on water quality in the Krishnagiri block of Tamil Nadu in south India:Fluoride.2000,33:121-7.
13.朱绯.高氟对斑马鱼造血基因Pul和cdx4的影响.[D]东北农业大学硕士学位论文.2007.
14.秦港.公鸡慢性氟中毒生殖毒性研究.[D]西南大学硕士学位论文.2006.
15.刘国艳.鸡氟中毒的毒理学研究.[D]东北农业大学博士学位论文.2000.
16.刘珊,吴天秀.氟化钠对大鼠血清微量元素的影响.[J]广东微量元素科学.2009,16(3)：22—26.
17.廖进民,陈艳.氟化物对幼年大鼠骨矿物质和血微量元素的影响机器相关性研究,2009,16(9)：13—19.
18.江琴.氟对雄性大鼠生殖细胞端粒酶表达影响的研究[D]郑州大学硕士学位论文.2005.
19.刘二龙.氟诱导原代大鼠肝细胞凋亡及bar基因表达研究[D]湖南农业大学硕士学位论文.2004.
20.贾丽红.氟致大鼠肝、肾细胞损伤机制及抗氧化营养素的干预性研究[D]中国医科大学博士学位论文.2002.
21.戴定戚,廖贤平.新生大鼠小肠上皮细胞分离培养研究[J].细胞生物学杂志.1997,19(1)：31-34.
22.陈思怀,刘燕.氟化物对培养猪小肠上皮细胞的影响[J].安徽农业科学.2008,36(3)：1062—1063，1098.
23.白焱,刘巍.水域氟污染与人体健康.[J]东北水利水电.2006,24(11)：268-269
24.朱其顺.中国地下水氟污染的现状及研究进展.[J]环境科学与管理.2009,34(1)：42-44
25.李凤华.地方性氟中毒对儿童智力发育的影响及氧化应激机制[D]南华大学硕士学位论文.2007
26.申秀英,章子贵,许晓路.氟对中枢神经系统的影响[J]中国地方病防治杂志.2001,16(6)：34
27.相有章,李明龙.现代慢性非传染性疾病预防与治疗[M]济南：山东科学技术出版社,2004：495.
28.邵红.地方性氟中毒患者骨骼损害及淋巴细胞基因表达的研究[D]新疆医科大学博士学位论文.2005.
29. Human Butyrylcholinesterase L330I Mutation Belongs to a Fluoride-Resistant Gene, by Expression in Human Fetal Kidney Cells. [J]Biochemical and Biophysical Research Communications 1997, (240):372-375.
30.张志民,任宝华,张家颖,高心,王成坤.变形链球菌耐氟菌株中耐酸相关基因dgk突变的检测及临床意义.[J]吉林大学学报：医学版.2008,34(5)：850-853.
31.姜葳,梁景平,李超伦,姜云涛,张明珠.远缘链球菌6715耐氟菌株与亲代菌株的基因表达差异.[J]上海交通大学学报：医学版,2007,27(2)：137-140.
32.林健荣,史奕山.氟素对家蚕幼虫血淋巴中酶活性及钙镁离子含量的影响.[J]蚕业科学.1994,20(2)：115-118.
33.沈海铭.桑蚕对氟化物的吸收排泄作用和抗氟性的关系.[J]绍兴文理学院学报.1997,17(6)：67-80.
34.沈海铭.桑蚕血液酸性磷酸酶活性和抗氟性关系的研究.[J]绍兴文理学院学报.1997,17(5)：51-54.
35.沈海铭.乙酰胆碱酯酶在桑蚕氟中毒中的表现以及和桑蚕抗氟性之间的关系.[J]绍兴文理学院学报.1998,18(6)：55-57.
36.陈志伟等.氟中毒家蚕过氧化氢酶活性的变化.[J]农村生态环境.2002,18(1)：31-34.
37.唐顺明,沈兴家等.家蚕雌雄性别耐氟性能差异的初步研究.[J]中国蚕业.2000(4)：16-17.
38. Chen YY,Wu YC.Effects of fluoride on changes of calcium,copper and ion level in the important organs of silkworm larvae(Bombyx mori).[J]Sericologia.1988,38(1):69-77.
39.王艳艳等.PCR-SSCP技术及其在植物研究中的应用.[J]生物信息学.2006,1：13-15.
40.唐顺明.蚕卵和氟中毒蚕体磷脂含量变化规律的研究[D]中国农业科学院硕士学位论文,2000.
41.杨明观,朱根生.以氟敏指数作度量指标的家蚕耐氟性测定法.[J]蚕业科学.1992,18(4)：227-231.
42.王章娥,吾布力艾合买提,刘明珠.家蚕耐氟性遗传与茧丝质性状相关性研究.[J]蚕桑通报.2005,36(1)：10-13.
43.刘伟强,林健荣,刘清神,陈芳艳,谢特新.人工饲料育蚕与桑叶育蚕对氟化物的抗性差异分析.[J]广东蚕业.2008,42(2)：16-18.
44.林昌麒,糜懿殿.家蚕耐氟显性主效基因的发现.[J]蚕业科学.1997,23(4)：237-239.
45.沈海铭.桑蚕抗氟性遗传模式的研究.[J]科技通报.1997,13(6)：419-422.
46.白会钗,徐安英,李木旺,赵云坡,郭秋红,钱荷英,张月华,赵远,黄勇平,郭锡杰.利用SSR标记对家蚕耐氟基因进行连锁定位分析.[J]蚕业科学.2008,34(2)：191-196.
47. Hongliang Zhou et al. Molecular cloning of Bombyx mori cytochrome P450 gene and its involvement in fluoride resistance.[J]Journal of Hazardous Materials.2008,779 (1):7-14.
48.周红亮,陈克平,姚勤,高路,陈慧卿,申红星,赵国力.家蚕bcl-2基因的克隆、序列分析及其表达模式.[J]农业生物技术学报.2008,16(3)：430-435.
49. Ranson H. Claudianos C, Ortelli F, Abgrall C, Hemingway J, Sharakhova M V, Unger M F, Collins FH and Feyereisen R. Evolution of supergene families associated with insecticide resistance. [J]Science (New York, N.Y).2002,298 (5591):179-181.
50. Claudianos C, Ranson H, Johnson RM, Biswas S, Schuler MA, Berenbaum MR, Feyereisen R and Oakeshott JG. A deficit of detoxification enzymes:pesticide sensitivity and environmental response in the honeybee. [J]Insect molecular biology.2006,15 (5):615-636.
51. Scott JG and Kasai S. Evolutionary plasticity of mono oxygenase-mediated resistance. Pesticide biochemistry and physiology. [J]2004,78 (3):171-178.
52. Scott JG, Liu N, Wen ZM. Insect cytoehromes P450:diversity insecticide resistance and tolerance to plant toxins.[J]Comp Biochem Physiol Part C,1998,121(1-3):147-155.
53. Scott JG,Zhimou Wen. Cytochromes P450 of insects:the tip of the iceberg.[J]PestManag Sci, 2001,57:958-967.
54.郭晶.乙酰胆碱酯酶的动力学机制及其应用[D]上海师范大学硕士学位论文.2007.
55. Feyereisen R. Insect P450 enzymes. Annual review of entomology.1999,44:507-533.
56.艾均文.家蚕(Bombyx mori)全基因组细胞色素P450基因结构与进化分析及CYP18A1的克隆与功能研究[D]西南大学博士学位论文.2007.
57. David R,Nelson. Metazoan cytochrome P450 evolution.[J]Comparative Biochemistry and Physiology,1998,121:15-22.
58. Feyereisen R.Evolution of insect P450.[J]Biochemical Society transactions.2006,34(6): 1252-1255.
59. Claudianos C, Ranson H, Johnson RM, Biswas S, Schuler MA, Berenbaum MR, Feyere-isen R and Oakeshott JG. A deficit of detoxification enzymes:pesticide sensitivity and en-vironmental response in the honeybee.[J]Insect molecular biology.2006,15(5):615-636.
60. Andersen JF, termoh len JGU et al. Expression of house fly CYP6A1 and NADPH-cytoc hrome P450 reductase in Escherichia coli and reconstitution of an insecticide metabolize-ng P450 system.[J]Biochemistry,1994,33 (8):2171-2177.
61. Tomita T, Scott JG. cDNA and deduced protein sequence of CYP6D1:the putative gene for a cytochrome P450 responsible for pyrethroid resistance in house fly. [J] Insect Bio-chem. Mol. Biol.1995(25):275-283.
62. Feyereisen R, Koener JF, Farnsworth DE,et al. Isolation and sequence of cDNA encodin g a cytochrome P450 from an insecticide-resistant strain of the house fly, Musca domest ica.[J]Proc Natl Acad Sci USA,1989(86):1465-1469.
63. Waters LC, Zelhof AC, Shaw BJ, et al. Possible involvement of the long terminal repe-at of transposable element 17.6 in regulating expression of an insecticide resistance-assoc iated P450 gene in Drosophila.[J] Proc. Natl. Acad. Sci. USA 1992 (89):4855-4859.
64. Cohen MB, Schuler MA, Berenbaum MR.A host-inducible cytochrome P450 from a host-specific caterpillar:molecular cloning and evolution.[J]Proc.Natl Acad.Sci.1992,90(12):564 3-5647
65.李斌,夏庆友,鲁成,等.家蚕细胞色素P450的基因组学分析.[J]中国科学C辑 生命科学2004,34(6)：517-521
66. Burgstalfler AW.Editorial:paradoxical dose-response effects of fluoride. Fluoride,2002,35:14 3-147.
67.刘清,杨克敌.氟化物与DNA损伤的研究进展[J].微量元素与健康研究,2003,20(6)：54—57.
68.谭红,张俊琴.氟中毒对免疫系统的影响[J].中华卫生监督与健康杂志,2002,1(2)：22—24.
69.张丹心,冯玉梅.氟诱发生殖激素失衡[J].国外医学,2004,25(3)：113—115.
70.许增禄,刘秉慈,缪庆.过量氟对大鼠骨骼I型胶原mRNA表达及纤维性状的影响.环境与健康杂志,2003,20(4)：204-206.
71.侯铁舟,李志丹.氟化物对体外器官培养人牙胚细胞增殖的相关调控蛋白表达的影响[J].西安交通大学学报,2003,24(4)：304—305.
72.王红,侯铁舟.氟化物作用下体外培养人牙胚内釉上细胞中Smad1、5表达的变化[J].口腔医学,2005,25(4)：201—204.
73.官志忠,王亚男.氟对SH-SY5Y神经细胞生物膜性脂质的作用[J].贵阳医学院学报,2005,30(1)：1—3.
74.闭立辉顾家栋胡乐山石美宁罗坚黄玲莉陆瑞好苏红梅韦博尤蒙艺英.家蚕抗氟品种“桂蚕F95”的选育研究[J].广西科学,2008,15(2)：198-204.
75.中华人民共和国国家标准牙膏GB8372-2001.
76.徐建峰许凌周卫龙.砖茶含氟量国家标准测定方法比较中国茶叶加工2007,(4)：47-49.
77. Leonard H Weinstein, Alan W Davison.Native plant species suitable as bioindicators and biomonitors for airborne fluoride. [J]Environmental Pollution 2003 125(1):3-11.
78.刘超,吴方正,傅柳松等.茶叶中的氟含量及测定方法研究[J].农业环境保护,1998,17(3)：132-135.
79.徐林生,顾晓山,翁荣林.桑树含氟量测定方法的讨论[J]江苏蚕业,1995,(3)：29-30.
80.徐建峰、周卫龙,茶叶中氟含量的测定.[J]中国茶叶加.2002(3)：35-36.
81.潘科,黄炳球.昆虫对有机磷类抗性机制研究进展.[J]世界农药,2001,23(2)：49-50.
82.郭晶.乙酰胆碱酯酶的动力学机制及其应用[D]上海师范大学硕士学位论文.2007.
83. Massoulie J, Pezzementi L, Bon S et al. Molecular and cellular biology of cholinesteras es. [J]Prog Neurobiol,1993,41:31
84. Taylor P, Radic Z Annu.The cholinesterases:from genes to proteins. [J]Rev Pharmacol T oxicol,1994,34:281
85. Rosenberry TL,Barnett P,Mays C.Acetylcholinesterase.[J]Areas Mol Biol,1982,43:103
86. Fallscheer HO, Cook JW, Assoc J. Studies on the conversion of some thionophosphates and a dithiophosphate to in vitro cholinesterase inhibitors. [J]Off Agr Chem,1956:692
87. Xianchun Li, Yinchang Wang.Progress in target-insensitive mechanism of insect resistance to insecticides. [J]Acta Entomologica Sinica.l998,41(4):417—425
88. Zhu KY,Lee SH,Clark JM.a point mutation of acetylcholinesterase associated with asinphosmethyl resistance and reduced fitness in Colorado potato betle. [J] Pest Biochem Physiol,1996,55:100—108.
89. Zhu KY,Clark JM.Validation of a point mutation of acetylcholinesterase in Colorado po-t ato beetle by polymerase chain reaction coupled to enzyme inhibition assay.[J]Pestle Bio chem Physiol,1997,57:28—35.
90. Fournier D,Bride JM,Hoffman F,et al.Acetylcholinesterase:two types ofmodifications confe r resistance to insecticide.[J] J Biol Chem,1992,267,14270—14274
91. Gao JR,Zhu KY.Comparative toxicity of selected organophasphate insecticides against resi stant and susceptible clones of the greenbug,Schizaphis graminum(Homoptera:aphididae).[J] J Agric Food Chem,2000,48:4717—4722.
92. Zhu KY,Gao JR.Increased activity associated with reduced sensitivity of acetylcholinester ase in organophosphate-resistant greenbug,Schizaphis graminum (Homoptera:Aphididae).[J] Pestic sci,1999,55:11—17.
93. Bradford MM. A rapid and sensitive method for the quantification of microgram quantiti es of protein utilizing the principle of protein dye binding.[J]Anal.B iochem.,1976,72:248.-254
94. Ellman GL, Courtny KD, Andres U.et al. A new and rapid colorimetric determination of acetylcholinesterase activity.[J]Biochem. Pharmacol.1961,(7):88—95
95. Swapnila Chouhana and SJS Flora. Effect of fluoride on the tissue oxidative stress and apoptosis in rats:Biochemical assays supported by IR spectroscopy data.[J]Tox-icology2008,254:61-67.
96. Eastbrook RW. The remarkerable P450s:A historical review of the versatile heme protein catalysis. [J]TASEB J,1996,10:202-204.
97. David R. Nelson. Metazoan cytochrome P450 evolution.[J]Comparative Biochemist-ry and Physiology,1998,121:15-22.
98. Feyereisen R. Evolution of insect P450.[J]Biochemical Society transactions.2006,34 (6):1252-1255.
99. Claudianos C, Ranson H, Johnson RM, Biswas S, Schuler MA, Berenbaum MR, Feyereisen R and Oakeshott JG. A deficit of detoxification enzymes:pesticide sensi-tivity and environmental response in the honeybee.[J]Insect molecular biology.2006, 15 (5):615-636.
100.Ranson H, Nikou D, Hutchinson M, Wang X, Roth CW, Hemingway J and Coll-ins FH. Molecular analysis of multiple cytochrome P450 genes from the malaria ve-ctor, Anopheles gambiae. [J]Insect molecular biology.2002,11 (5):409-418.
101.艾均文,董元凌,孔卫青等.细胞色素P450新家族的第一个基因CYP337A1的分子克隆与序列分析[J].西南大学学报(自然科学版),2008,30(2)：51-58.
102.吴明玮.白蚊伊蚊乙酰胆碱酯酶基因片段简并引物PCR、克隆及鉴定[D]暨南大学硕士学位论文.2001.
103.Daborn P, Boundy S, Yen J, Pittendrigh B, and ffrench-Constant R.DDT resistance in Drosophila correlates with Cyp6gl over-expression and confers cross-resistance to the ne onicotinoid imidacloprid[J]Mol Genet Genomics.2001,266(4):556-563.
104.尚金燕.家蚕两种类型乙酰胆碱酯酶基因的分析[D]浙江大学博士学位论文.2006.
105.张永亮.野桑蚕和桑尺蠖酚氧化酶的生化特性、基因克隆、表达及功能研究[D]西南大学博士学位论文.2007.
106.陈艳霞.家蝇乙酰胆碱酯酶基因的克隆、突变、表达与应用研究[D]浙江大学硕士学位论文.2007.
107.陈茂华.两种麦蚜乙酰胆碱酯酶的分子生物学研究[D]南京农业大学博士学位论文.2005.
108.施明安.果蝇和家蝇乙酰胆碱酯酶的表达与功能研究[D]中国科学院研究生院博士学位论文.2003.
109.方晓东.家蝇乙酰胆碱酯酶基因的克隆、表达及三维结构预测分析[D]华南热带农业大学硕士学位论文.2006.
110.彭霞.农药靶标乙酰胆碱酯酶的分离纯化及性质研究[D]四川大学硕士学位论文.2007.
111.柴玉鑫.嗜卷书虱乙酰胆碱酯酶生化毒理学及其基因克隆与序列分析[D]西南大学硕士学位论文.2007.
112.吴明玮等.白纹伊蚊乙酰胆碱酯酶基因片段克隆及鉴定.[J]中国寄生虫学与寄生虫病杂志；2001,19(4)：209-212.
113.吴明玮等.白纹伊蚊乙酰胆碱酯酶基因片段序列分析.[J]中国寄生虫学与寄生虫病杂志,2002,20(3)：187.
114.李春晓等.淡色库蚊乙酰胆碱酯酶全基因的克隆鉴定及蛋白同源模拟.[J]寄生虫与医学昆虫学报,2004,1l(3)：161-165.
115.丁中等.甘薯茎线虫乙酰胆碱酯酶基因ace-3全长cDNA的克隆和序列分析.[J]农业生物技术学报,2008,16(2)：326-331.
116.丁中等.甘薯茎线虫乙酰胆碱酯酶基因Dd-ace-2全长cDNA的克隆和序列分析.[J]生物工程学报,2008,25(2)：239-244.
117.林同等.橄榄星室木虱乙酰胆碱酯酶基因的克隆与序列分析.[J]吉林农业大学学报,2007,29(4)：368-370.
118.陈茂华等.禾谷缢管蚜、麦长管蚜乙酰胆碱酯酶基因片段的克隆与序列分析.[J]南京农业大学学报.2006,29(1)：35-39.
119.杨之帆等.褐飞虱乙酰胆碱酯酶基因全长cDNA的克隆及序列分析.[J]昆虫学报,2006,49(6)：1034-1041.
120.方晓东等.家蝇乙酰胆碱酯酶基因的克隆与序列分析.[J]中国热带医学.2006.,6(6)：59-62.
121.李昕等.家蝇中乙酰胆碱酯酶的提取纯化及应用研究.[J]分析试验室,2008,27(3)：87-90.
122.陶卉英等.嗜卷书虱抗性与敏感品系乙酰胆碱酯酶的比较.[J]西南农业大学学报,2005,27(2)：151-154.
123.王敦等.昆虫乙酰胆碱酯酶基因研究进展.[J]昆虫学报,2006,49(3)：497-503.
124.黄晓等.天然产物对家蚕乙酰胆碱酯酶抑制作用研究.[J]四川动物,200,27(3)：23-26.
125.丁中等.线虫乙酰胆碱酯酶研究进展.[J]植物保护.2008,.34(3)：81-84.
126.徐恩斌等.乙酰胆碱酯酶的研究进展.[J]国外医学·生理、病理科学与临床分册.2003,23(1)：73-75.
127.赵建庄等.乙酰胆碱酯酶分离纯化的方法.[J]北京农学院学报,2003,18(4)：249-251.
128.张明等.乙酰胆碱酯酶分子生物学研究进展.[J]农药.2006,45(1)：8-11.
129.魏福祥等.乙酰胆碱酯酶固定化方法的研究.[J]化学世界.2005,3：51-55.
130.刘铭钧等.乙酰胆碱酯酶基因工程技术研究进展.[J]生物技术.2008,18(2)：84-87.
131.张千等.乙酰胆碱酯酶生物功能的研究进展及其应用.[J]国外医学卫生学分册.2008,35(3)：143-146.
132.王小瑜等.乙酰胆碱酯酶试剂包配方的选择.[J]食品安全.2007,6：38-41.
133.奉水东等.PCR-SSCP检测肺癌EGFR基因突变.[J]现代检验医学杂志.2008,23(2)：13-15.
134.于成江等.常年性和季节性发情绵羊品种HIOMT基因的PCR-SSCP分析.[J]西北农林科技大学学报,2008,36(5)：31-35.
135.李春晓等.淡色库蚊乙酰胆碱酯酶基因上的可变剪接及对酶活性的影响.[J]寄生虫与医学昆虫学报,2007,14(3)：351-355.
136.颜鑫等.多作用点乙酰胆碱酯酶抑制剂研究进展.[J]中南药.2007,5(5)：397-401.
137.杨之帆等.褐飞虱乙酰胆碱酯酶基因全长cDNA的克隆及序列分析.[J]昆虫学报.2006,49(6)：1034-1041.
138.孙晓琴等.家蝇和果蝇的靶标抗性研究进展.[J]农药.2008,47(11)：785-789.
139.方晓东等.家蝇乙酰胆碱酯酶基因的克隆与序列分析.[J]中国热带医学.2006,6(6)：59-61.
140.李昕等.家蝇中乙酰胆碱酯酶的提取纯化及应用研究.[J]分析试验室.2008,27(3)：87-90.
141.李飞等.抗性品系棉蚜乙酰胆碱酯酶和羧酸酯酶的变异.[J]昆虫学报.2003,46(5)：578—583.
142.陶卉英等.嗜卷书虱抗性与敏感品系乙酰胆碱酯酶的比较.[J]西南农业大学学报,2005,27(2)：151-154.
143.潘科等.昆虫对有机磷类抗性机制研究进展.[J]世界农药.200123(2)：49-50.
144.曲明静等.昆虫乙酰胆碱酯酶基因变异抗药性机制研究.[J]昆虫知识.2007,44(2)：191-194.
145.王敦等.昆虫乙酰胆碱酯酶基因研究进展.[J]昆虫学报.2006,49(3)：497-503.
146.彭霞等.农药靶标乙酰胆碱酯酶的分离纯化及性质研究.[J]四川大学学报.2008,45(1)：90-95.
147.刘泽文等.羧酸酯酶和乙酰胆碱酯酶在褐飞虱对甲胺磷抗性发展中的作用.[J]农药学学报.2002,4(4)：51-55.
148.黄晓等.天然产物对家蚕乙酰胆碱酯酶抑制作用研究.[J]四川动.2008,27(3)：723-726.
149.吴明玮等.蚊乙酰胆碱酯酶基因突变与抗药性.[J]国外医学寄生虫病分册.2000,27(5)：201-204.
150.丁中等.线虫乙酰胆碱酯酶研究进展.[J]Plant Protection.2008,34(3):81-84.
151.王建军等.小菜蛾乙酰胆碱酯酶cDNA片段的克隆和序列分析.[J]昆虫学报.2002,45(4)：544-547.
152.张耀东等.乙酰胆碱酯酶的定点突变法设计及其在生物传感器领域的应用.[J]生物技术通报,2006,5：80-83.
153.郭晶等.乙酰胆碱酯酶的动力学机制及其应用.[J]农药,2007,46(1)：18-21.
154.徐恩斌等.乙酰胆碱酯酶的研究进展.[J]国外医学·生理、病理科学与临床分册,2003,23(1)：73-75
155.赵建庄等.乙酰胆碱酯酶分离纯化的方法.[J]北京农学院学报,2003,18(4)：249-251.
156.张明等.乙酰胆碱酯酶分子生物学研究进展.[J]农药,2006,45(1)：8-11.
157.魏福祥等.乙酰胆碱酯酶固定化方法的研究.[J]化学世界,2005,3：551-555.
158.刘铭钧等.乙酰胆碱酯酶基因工程技术研究进展.[J]生物技术..2008,18(2)：84-86.
159.张千等.乙酰胆碱酯酶生物功能的研究进展及其应用.[J]国外医学卫生学分册.2008,35(3)：143-147
160.刘芳等.乙酰胆碱酯酶性质改变与昆虫抗药性的关系.[J]华东昆虫学报2006.15(3)：187-191.
161.林建国等.与昆虫抗药性相关的乙酰胆碱酯酶基因突变研究进展.[J]农药学学报.2005,7(1)：01.06.
162.钟仰进等.氟化物引起家蚕的组织病变[J]华南农业大学学报.1994,15(1)：18-21.
163.王安皆,娄齐年,周丽霞,张凤林,聂磊,王娜.家蚕多丝量品种960l×92的耐氟性试验.[J]北方蚕业,2008,29(4)：18-19.
164.闭立辉,顾家栋,石美宁,罗坚,黄玲莉,苏红梅,韦博尤,蒙艺英.家蚕耐氟新品种“桂蚕F95"的主要性状.[J]广西蚕业,2008,45(2)：26-28.
165.郭定国,林健荣,黄嫔,林忠芬,钟苏苑，邱国祥,李林山,倪惠波,张桂玲,李惠华.家蚕新品种粤枫三号(五·选×28·32)的育成.[J]蚕业科学,2007,33(3)：466-469.
166.徐安英,林昌麒,侯成香,张月华,李木旺,孙平江,钱荷英.家蚕春用耐氟品种华源×东升的选育及其性状.[J]中国蚕业,2006,27(2)：30-32.
167.徐庆刚,陈克平,姚勤,陆健.家蚕耐氟基因RAPD分子标记的筛选及其克隆.[J]生物技术,2004,14(3)：10-11.
168.吾布力.艾合买提,王章娥,刘明珠.家蚕不同世代耐氟性比较及遗传分析_[J]中国蚕业,2004,25(3)：22-23.
169.赵巧玲,袭智勇,夏定国,章佩祯,潘新平,林方华,戴建一.耐氟蚕品种秋丰、白玉改良系的特点.[J]中国蚕业,2003,24(4)：54-55.
170.张升祥,王彦文,吴绪东,李春英,宋雪艳.山东家蚕现行品种耐氟性测定.[J]山东农业科学,2003(2)：39-40.
171.张士英,冯建琴,陈健康,沈建华,丁农,李玉峰,金卫明.家蚕春秋兼用耐氟杂交组合华·秋×松·白的选配.[J]蚕业科学,2003,29(3)：318-319.
172.姚耀涛,陈端豪.家蚕品种耐氟性比较.[J]中国蚕业,2002,23(3)：65-66.
173.沈建华.菁松F×皓月F的耐氟性测定及其茧丝质成绩比较.[J]中国蚕业,.2002,23(1)：27-29.
174.沈兴家,李奕仁等.春秋兼用耐氟蚕品种华峰GW×雪·A的选配.[J]蚕业科学,2002,28(1)：52-55.
175.姚勤,陈克平.家蚕分子标记辅助育种.[J]江苏蚕业,2002,24(3)：5-8.
176.张烈,鲁成等.陕西家蚕品种资源耐氟性调查初报.[J]西南农业大学学报.,2001,23(1)：26-28.
177.魏兆军,王章娥等.家蚕耐氟性状基因效应分析.[J]蚕业科学,2001,27(2)：158-161.
178.陈玉银.家蚕耐氟性差异的细胞化学研究.[J]昆虫学报,2000,43(3)：271-279.
179.林昌麒,陈克平.家蚕耐氟种质的创建.[J]蚕学通讯,2000,20(2)：1-7.
180.古巧珍,李亚清.若干家蚕基础品种的耐氟性测定.[J]北方蚕业,1999,20(1)：16-17.
181.王章娥,曹志清.家蚕品种间耐氟性比较及生化分析.[J]蚕业科学,1999,25(4)：258-260.
182.唐顺明,池兴家.蚕品种耐氟性能试验.[J]江苏蚕业,1998(1)：62-63.
183.林昌麒,糜懿殿.家蚕耐氟显性主效基因的发现.[J]蚕业科学,1997,23(4)：237-239.
184.林昌麒,糜懿殿.家蚕耐氟遗传初探.[J]江苏蚕业,1997,19(2)：7-10.
185.周金妹,杨明观.以氟敏指数为指标的家蚕耐氟性选择效果.[J]浙江农业大学学报,1996,22(4)：437-438.
186.刘晓勇,陈克平,姚勤,李军,蔡克亚.家蚕中肠组织抗核型多角体病毒病的相关蛋白分析.[J]昆虫学报,2008,51(4)：443-448.
187.蔡克亚,陈克平,刘晓勇,姚勤,李军.家蚕抗BmNPV品系与感性品系血淋巴液蛋白质组的差异分析.[J]生物工程学报,2008,24(2)：285-290.
188.赵国力,陈克平,姚勤,郭忠建.家蚕生殖细胞相关基因nanos的克隆表达及在胚胎发育中的表达模式.[J]昆虫学报,2007,50(11)：1092-1098.
189.高贵田,陈克平,姚勤,王林玲,陈慧卿.家蚕羧酸酯酶基因克隆及差异表达.[J]昆虫学报,2006,49(6)：930-937.
190.高贵田,陈克平,姚勤,徐家萍,王林玲,陈慧卿.家蚕eIF2a全长cDNA克隆及生物信息学分析.[J]中国农业科学.2006,39(5)：1031-1037.
191.陈克平,鲁成.分子连锁图与家蚕育种.[J]江苏蚕业,1999,21(3)：1-4.
192. Ivana Gazic et al. Preparative HPLC separation of bambuterol enantiomers and stereoselective inhibition of human cholinesterases.[J] Anal Bioanal Chem 2006.385:1513-1519.
193.任宝华.变形链球菌耐氟菌株中耐酸相关基因dgk和dltC突变的选择.[D]吉林大学硕士学位论文.2006
194.张磊.茶树氟吸收动力学特性的研究.[D]中国农业科学院硕士学位论文.2008
195.李加美.氟对体外培养血管内皮细胞功能的影响.[D]山东大学硕士学位论文.2007
196.仲伟丽.氟砷暴露对儿童智力水平及生长发育影响的调查分析.[D]山西医科大学硕士学位论文.2006
197.王锡林.氟致雄性大鼠生殖损害及硒对其拮抗作用.[D]郑州大学硕士学位论文.2002
198.梁大刚.氟中毒对家蚕幼虫体内谷胱甘肽(GSH)代谢的影响.[D]浙江大学硕士学位论文.2004
199.宁红梅.高氟低碘对老年大鼠不同脏器细胞DNA损伤的研究.[D]山西农业大学硕士学位论文.2004
200.向泉永.环境中氟对居民健康影响的危险度评价.[D]复旦大学博士学位论文.2004
201.李兴霞.鸡实验性慢性氟中毒致畸致变的研究.[D]西南农业大学硕士学位论文.2004
202.周红亮.家蚕cyp306al和bcl-2基因的鉴定及其耐氟性功能研究.[D]江苏大学硕士学位论文.2008
203.尚金燕.家蚕两种类型乙酰胆碱酯酶基因的分析.[D]浙江大学博士学位论文.2006
204.何琳.家蚕神经系统靶点基因克隆与功能研究.[D]中国科学院研究生院博士学位论文.2007
205.陈松等.6种增溶剂对棉铃虫细胞色素P450 O.脱甲基活性的影响.[J]南京农业大学学报2003,26(4)：127-129
206.韩雅莉等.疣荔枝螺微粒体细胞色素P450酶系的光谱分析.[J]光谱学与光谱分析.2006,26(1)：130-133.
207.曾晓等.德国小蠊抗性与敏感品系细胞色素p450生化特征的研究.[J]中国媒介生物学及控制杂志.2004,15(3)：175-179.
208.王爱红等.氯乙烯染毒对大鼠肝细胞色素P4502E1活力和mRNA表达的影响.[J]工业卫生与职业病.2005,31(3)：146-148.
209.郑明奇等.棉铃虫中肠微粒体p450的分离纯化.[J]昆虫学报2005,48(3)：359-364.
210.陈松等.不同种群棉铃虫三龄幼虫酯酶活性频率分布与抗药性的关系.[J]昆虫学报.2002,45(6)：733-738
211.胡小邦,朱昌亮.昆虫抗药性机制研究进展.[J]国外医学寄生虫病分册.2004,31(1)：18-23.
212_吴益东等.棉铃虫抗药性监测方法——浸叶法敏感毒力基线的建立及其应用.[J]昆虫学报,.2001,44(1)：56-61.
213.于彩虹,高希武,郑炳宗.2-十三烷酮对棉铃虫细胞色素p450的诱导作用.[J]昆虫学报.2002,45(1)：1-7.
214.马彩霞.棉铃虫细胞色素P450基因的克隆与表达[D]西北农林科技大学博士学位论文.2005
215.黄剑.小菜蛾抗阿维菌素品系细胞色素P450的研究.[D]西北农林科技大学博士学位论文.2005
216.陈松.与氰戊菊酯抗性相关的棉铃虫细胞色素P450基因的克隆及异源表达.[D]南京农业大学博士学位论文.2005
217.陈秋霞等.昆虫细胞色素P450的诱导和抗药性.[J]热带医学杂志2001,1(1)：67-70.
218.邱星辉.昆虫细胞色素P450研究的一些新进展.[J]昆虫知识,2003.40(6)：487-491.
219.张听等.昆虫细胞色素P450与抗药性关系的分子生物学研究进展.[J]中国寄生虫病防治杂志,2002,15(1)：62-64.
220.于彩虹等.棉铃虫细胞色素P450CO差光谱的测定.[J]昆虫学报.2005,48(2)：301—304.
221.郑明奇等.棉铃虫中肠微粒体P450的分离纯化.[J]昆虫学报.2005,48(3)：359—364.
222.张永强等.嗜卷书虱抗气性和抗药性品系呼吸代谢的比较.[J]西南农业大学学报,2003,25(5)：413-416.
223.曹俊等.嗜人按蚊溴氰菊酯抗性分子机理研究：Ⅲ嗜人按蚊P450基因mRNA荧光半定量RT-PCR分析.[J]中国血吸虫病防治杂志.2005,17(6)：433-436.
224.郑明奇等.细胞色素P450s酶系催化除草剂代谢的研究进展[J]农药学学报.2003,5(3)：1-8.
225.赵博等.细胞色素P450的研究进展.[J]山东农业大学学报(自然科学版),2004,35(I)：142-144.
226.吕敏等.细胞色素P450酶系与苦皮藤素V对昆虫选择毒性的关系研究.[J]西北农林科技大学学报,2003,31(3)：35-38.
227.朱大岭等.细胞色素P450酶系在药物代谢中的作用.[J]医药导报2004.,23(7)：440-443.
228.陈玉华等.细胞色素P450在家蚕中的研究.[J]江苏蚕业,2005,2：1-3
229.刘小宁等.应用毛细管气相色谱法检测棉铃虫细胞色素P450 O-脱甲基活性.[J]昆虫知识.2004,41(3)：232-235.
230.周国理等.昆虫CYP6家族多样性与进化.[J]昆虫知识，2002，39(4)：246-251.
231.邱星辉等.昆虫CYP6家族多样性与进化.[J]昆虫知识,2003,40(6)：487-491.
232.陈婕等.细胞色素P450氧化还原酶的研究进展.[J]中国药理学通报.2006,22(2)：129-133.
233.刘德立等.细胞色素P450与农药相互作用及其机理研究.[J]华中师范大学学报,2005,39(4)：518-523.
234.邱星辉等.细胞色素P450在植物与昆虫相互关系中的作用.[J]昆虫知识.2002,39(1)：5-8.
235.周国理等.白纹伊蚊CYP6N3基因启动子序列的分子克隆与功能分析.[J]寄生虫与医学昆虫 学报,2002,9(1)：12-20.
236.吴瑜等.白纹伊蚊基因组中CYP6家族新成员基因片段的克隆与分析.[J]寄生虫与医学昆虫学报.2001,8(1)；23-30.
237.周国理等.白纹伊蚊溴氰菊酯抗性株基因组中CYP6基因片段的分离与鉴定.[J]中国人兽共患病杂志.2000,16(1)：22-26.
238.王立新等.草鱼MyoD cDNA的克隆和序列分析.[J]中国农业科学.2005,38(10)：2134-2138.
239.肖勇梅等.巢式PCR法构建人细胞色素P4501A1 基因 cDNA全长T载体.[J]广东医学,2005,26(8)：1046-1049.
240.腾达等.淡色库蚊抗溴氰菊酯品系CYP4家族新成员基因克隆及序列分析.[J]中国寄生虫病防治杂志.2004,17(2)：65-68.
241.李秀兰等.淡色库蚊细胞色素P450 CYP4E2r6基因的克隆、表达及鉴定.[J]中国人兽共患病杂志.2005,21(7)：552-558.
242.朱昌亮等.淡色库蚊细胞色素p450基因研究.[J]生物化学与生物物理进展.2001：28(1)：61-67.
243.申望等.腐生型眼虫Astasia longa两种TIM同工酶cDNA克隆分析.[J]动物学报.2004,50(3)：414-419.
244.朱礼华等.家蝇抗性相关p450基因的初步研究.[J]寄生虫与医学昆虫学报,2000,7(2)：99-103.
245.艾颖等.棉铃虫细胞色素P450 cDNA片段的克隆与序列分析.[J]昆虫知识,200441(3)：227-232.
246.黄炯烈等.蚊虫细胞色素p450基因系列研究.[J]中山医科大学学报.2001.,22(6)：401-405,422.
247.李洪山等.小菜蛾细胞色素P450基因的cDNA片段克隆及其序列分析.[J]江苏农业学报,2006,22(1)：29-32.
248.王东兰等.紫茎泽兰CYP75基因cDNA片段的克隆与鉴定.[J]植物保护.2005,31(4)：65-70.
249. Guo chong Zhi et al. Molecular simulation of a single-chain antibody against AChE to explore molecular basis of inhibitory effect of 3F3M cAb on enzyme activity.[J]Acta Pharmacol Sin 2003,24(5):460-466.
250. Nannan Liu et al. CYP4AB1, CYP4AB2, and Gp-9 gene overexpression associated with workers of the red imported fire ant, Solenopsis invicta Buren.[J]Gene.2004,327:81-87.
251. Nannan Liu et al. Differential expression of genes in pyrethroid resistant and susceptible mosquitoes, Culex quinquefasciatus (S.).[J] Gene.2007,394:61-68.
252. Nannan Liu et al. Identification of two new cytochrome P450 genes and their 5_flanking regions from the housefly, Musca domestica. [J] Insect Biochemistry and Molecular Biology.2002,32:755-764.
253. Qiang Xu et al. Kdr allelic variation in pyrethroid resistant mosquitoes, Culex quinquefasciatus (S.).[J] Biochemical and Biophysical Research Communications.2006,345:774-780.
254. Julia W. Pridgeon, Nannan Liu.Overexpression of the cytochrome c oxidase subunit I gene associated with a pyrethroid resistant strain of German cockroaches, Blattella germanica (L.)[J] Insect Biochemistry and Molecular Biology.2003,33:1043-1048.
255. Qiang Xu et al.Sodium channel gene expression associated with pyrethroid resistant house f lies and German cockroaches.[J] Gene.2006,379:62-67.
256. Helvig C et al.CYP15A1, the cytochrome P450 that catalyzes epoxidation of methyl farnesoate to juvenile hormone Ⅲ in cockroach corpora allata.[J] PNAS.2004,101(12):4024-4029.
257. Claudianos C et al. A deficit of detoxification enzymes:pesticide sensitivity and environmental
response in the honeybee[J]Insect Molecular Biology.2006,15 (5):615-636.
258. Srividya Bhaskara et al. Caffeine induction of Cyp6a2 and Cyp6a8 genes of Drosophila melanogaster is modulated by cAMP and D-JUN protein levels.[J] Gene.2008,415:49-59.
259. Cynthia J Coffman et al. Identification of coregulated transcripts affecting male body size in Drosophila.[J]Genome Biology.2005,6:53.
260. Hongshan Li et al. Molecular cloning and nucleotide sequence of CYP6BF1 from the diamondback moth, Plutella xylostella[J] JIS.2005,5:45.
261. Sutherland TD et al. A cytochrome P450 terpenoid hydroxylase linked to the suppression of insect juvenile hormone synthesis.[J] Proc. Nati. Acad. Sci.1998,95:12884-12889.
262. Michael B.Cohen et al. A Host-Inducible Cytochrome P450 from a Host-Specific Caterpillar-Molecular Cloning and Evolution.[J] Proc.Nati.Acad.Sci.1992,89:10920-10924.
263. Michael R. Bogwitz, Henry Chung, Lorin Magoc et al.Cypl2a4 confers lufenuron resistance in a natural population of Drosophila melanogaster. [J] PNAS.2005,102(36):12807-12812.
264. Thomas L. Poulos Cytochrome P450 flexibility.[J] PNAS.2003,100 (23):13121-13122.
265. Mudgapallia shok et al. Insect juvenile hormone resistance gene homology with the bHLH-PAS family of transcriptional regulators.[J]Proc.Natl.Acad.Sci.1998,95:2761-2766.
266. Thomas G. Wilson et al. Insecticide resistance resulting from an absence of target-site gene product.[J] Proc.Natl.Acad.Sci.1998,95:14040-14044.
267. Reni Feyereisen et al. Isolation and Sequence of cDNA Encoding a Cytochrome P-450 from an Insecticide-Resistant Strain of the House Fly, Musca domestica. [J]Proc.Natl.Acad.Sci. 1989,86:1465-1469.
268. Tetsuro Shinoda et al. Juvenile hormone acid methyltransferase--A key regulatory enzyme for insect metamorphosis.[J] PNAS.2003,100(21):11986-11991.
269. James T. Warren et al. Molecular and biochemical characterization of two P450 enzymes in the ecdysteroidogenic pathway of Drosophila melanogaster.[J] PNAS.2002,99(17):11043-11048.
270. Phillip B, Danielson et al. Molecular cloning of a family of xenobiotic-inducible drosophilid cytochrome P450s-Evidence for involvement in host-plant allelochemical resistance.[J] Proc. Natl. Acad.Sci.1997,94:10797-10802.
271. Stefan Jennewein et al. Random sequencing of an induced Taxus cell cDNA library for identification of clones involved in Taxol biosynthesis. [J] PNAS.2004,101(24):9149-9154.
272. Anna Petryk et al. Shade is the Drosophila P450 enzyme that mediates the hydroxylation of ecdysone to the steroid insect molting hormone 20-hydroxyecdysone.[J] PNAS.2003,25(100):13773-13778.
273. Xianchun Li et al.Structural and functional divergence of insect CYP6B proteins-From specialist to generalist cytochrome P450.[J] PNAS.2004,101(9):2939-2944.
274. Jean-Philippe David et al. The Anopheles gambiae detoxification chip-A highly specific microarray to study metabolic-based insecticide resistance in malaria vectors.[J]PNAS.2005, 102(11):4080-4084.
275. Randy L,Rose et al. Cytochrome P450-Associated Insectici de Resistance and the Development of Biochemical Diagnostic Assays in Heliothis virescens.[J]Pesticide Biochemistry and Physiology.1995,51:178-191.
276. Yu SJ et al.Biochemical characteristics of insecticide resistance in the fall armyworm, Spodoptera frugiperda (J.E. Smith).[J]Pesticide Biochemistry and Physiology.2003,77:1-11.
277. Renaud Le Bouquin et al.CYP94A5, a new cytochrome P450 from Nicotiana tabacum is able to catalyze the oxidation of fatty acids to the-alcohol and to the corresponding diacid.[J]Eur. J. Biochem.2001,268:3083-3090.
278. Min-SooYun et al.Cytochrome P-450 monooxygenase activity in herbicide-resistant and-susceptible late watergrass (Echinochloa phyllopogon).[J]Pesticide Biochemistry and Physiology.2005,83:107-114.
279. Christiane N,Gouamene-Lamine et al.Differential susceptibility to abamectin and two bioactive avermectin analogs in abamectin-resistant and susceptible strains of Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera:Chrysomelidae).[J]Pesticide Biochemistry and Physiology.2003,76:15-23.
280. Chansak Suwanchaichinda et al.Effects of Exposure to Pesticides on Carbaryl Toxicity and Cytochrome P450 Activities in Aedes albopictus Larvae.[J]Pesticide Biochemistry and Physiology.2001,70:63-73.
281. Sivapiriya V et al. Evects of dimethoate (0,0-dimethyl S-methyl carbamoyl methyl phosphorodithioate) and Ethanol in antioxidant status of liver and kidney of experimental mice.[J] Pesticide Biochemistry and Physiology.2006,85:115-121.
282. Anita Ma, Bautista M et al.Identification of permethrin-inducible cytochrome P450s from the diamondback moth, Plutella xylostella (L.) and the possibility of involvement in permethrin resistance.[J]Pesticide Biochemistry and Physiology.2006,85:1-9.
283. Emmanouil Roditakis et al.IdentiWcation of mutations in the para sodium channel of Bemisia tabaci from Crete, associated with resistance to pyrethroids.[J]Pesticide Biochemistry and Phy siology.2006,85:161-166.
284. David JP et al.Involvement of cytochrome P450 monooxygenases in the response of mosquito larvae to dietary plant xenobiotics.[J]Insect Biochemistry and Molecular Biology.2006,36:410-420.
285. Tsagkarakou A et al. Mechanisms of resistance to organophosphates in Tetranychus urticae (Acari-Tetranychidae) from Greece. [J]Insect Biochemistry and Molecular Biology.2002,32:417-424.
286. Si-Woo Lee.Metabolic resistance mechanisms of the housefly(Musca domestica) resistant to pyraclofos.[J]Pesticide Biochemistry and Physiology.2006,85:76-83.
287. Rachel L. Cox et al.Nitric oxide synthase sequences in the marine fish Stenotomus chrysops and the sea urchin Arbacia punctulata, and phylogenetic analysis of nitric oxide synthase calmodulin-binding domains.[J]Comparative Biochemistry and Physiology.2001,130:479-491.
288. Tomoko Takeda et al.Parasitization by Cotesia plutellae enhances detoxifying enzyme activity in Plutella xylostella.[J] Pesticide Biochemistry and Physiology.2006,86:15-22.
289. Katsuya Natsuhara et al.Phenobarbital induction of permethrin detoxification and phenobarbital metabolism in susceptible and resistant strains of the beet armyworm Spodoptera exigua (Hiibner).[J] Pesticide Biochemistry and Physiology.2004,79:33-41.
290. Yang Y et al.The involvement of microsomal oxidases in pyrethroid resistance in Helicoverpa armigera from Asia.[J]Insect Biochemistry and Molecular Biology.2004,34:763-773.
291. Julia W. Pridgeon et al.Variability of resistance mechanisms in pyrthroid resistant German cockroaches.[J]Pesticide Biochemistry and Physiology.2002,73:149-156.
292. Jeffrey G. Scott.Cytochromes P450 and insecticide resistance.[J]Insect Biochemistry and Molecular Biology.1999,29:757-777.
293. Chansak Suwanchaichinda et al. Effects of Exposure to Pesticides on Carbaryl Toxicity and Cytochrome P450 Activities in Aedes albopictus Larvae.[J]Pesticide Biochemistry and Physiology.2001,70:63-73.
294. Craig Sams et al. Evidence for the activation of organophosphate pesticides by cytochromes P450 3A4 and 2D6 in human liver microsomes.[J]Toxicology Letters.2000,116:217-221.
295. Si-Woo Lee et al.Metabolic resistance mechanisms of the housefly (Musca domestica) resistant to pyraclofos.[J]Pesticide Biochemistry and Physiology.2006,85:76-83.
296. Yang Y et al.The involvement of microsomal oxidases in pyrethroid resistance in Helicoverpa armigera from Asia.[J]Insect Biochemistry and Molecular Biology.2004,34:763-773.
297. Janet Hemingway et al.The molecular basis of insecticide resistance in mosquitoes.[J]Insect Biochemistry and Molecular Biology.2004,34:653-665.
298. Scharf ME et al. Expression and induction of three family 4 cytochrome P450 genes identified from insecticide resistant and susceptible western corn rootworms Diabrotica virgifera virgifera.[J] Insect Molecular Biology.2001,10(2):139-146.
299. Festucci-Buselli RA et al.Expression of Cyp6g1 and Cyp12d1 in DDT resistant and susceptible strains of Drosophila melanogaster.[J]Insect Molecular Biology.2005,14(1):69-77.
300. Shinji Kasai et al.Molecular cloning, nucleotide sequence and gene expression of a cytochrome P450 (CYP6F1) from the pyrethroid-resistant mosquito, Culex quinquefasciatus Say.[J]Isect Biochemistry and Molecular Biology.2000,30:163-171.
301. Shinji Kasai et al. Male specific expression of a cytochrome P450 (Cyp312al) in Drosophila melangaster.[J]Biochemical and Biophysical Research Communications.2003,300:894-900.
302. Shinji Kasai et al. Evolutionary plasticity of monooxygenase-mediated resistance[J] Pesticide Biochemistry and Physiology.2004,78:171-178.
303. Shinji Kasai et al.P450 Monooxygenases Are an Important Mechanism of Permethrin Resistance in Culex uinquefasciatus Say Larvae.[J]Archives of Insect Biochemistry and Physiology.1998,37:47-56.
304. Jeffrey G. Scott et al House-fly cytochrome P450 CYP6D1:5'flanking sequences and comparison of alleles.[J]Gene.1999,226:347-353.
305. Shinji Kasai et al.Overexpression of Cytochrome P450 CYP6D1 Is Associated with Monooxygenase-Mediated Pyrethroid Resistance in House Flies from Georgia.[J]Pesticide Biochemistry and Physiology.2000,68:34-41.
306. Shinji Kasai et al. A House Fly Gene Homologous to the Zinc Finger Proto-oncogene Gfi-1.[J] Biochemical and Biophysical Research Communications.2001,283:644-647.
307. Kasai S et al. Molecular cloning and nucleotide sequence of a cytochrome P450 cDNAfrom a pyrethroid-resistant mosquito,Culex quinquefasciatus Say.[J]Insect Molecular Biology.1998,30: 185-190.
308. Li Zhang et al.In Vitro Metabolism of Pyriproxyfen by Microsomes FromSusceptible and Resistant Housefly Larvae.[J] Archives of Insect Biochemistry and Physiology.1998,37:215-224
309. Toshio Shono et al.Genetics and mechanisms of permethrin resistance in the YPER strain of house fly.[J]Pesticide Biochemistry and Physiology.2002,73:27-36.
310. Kelsey A.Milleret alAssessment of cytochrome P450 1A in harbour seals (Phoca vitulina) using a minimally-invasive biopsy approach.[J]Marine Environmental Research.2005,60:153-169.
311. Randy Rose et al.Cytochrome P450 (CYP)9A1 in Heliothis virescens-the first member of a new CYP family.[J]Insect Biochemistry.1997,279(6):605-615.
312. Omprakash Mittapalli et al.Differential expression of two cytochrome P450 genes in compatible and incompatible Hessian fly-wheat interactions. [J]Insect Biochemistry and Molecular B iology.2005,35:981-989.
313.Nannan Liu et al.Identification of two new cytochrome P450 genes and their 5'-flanking regions from the housefly, Musca domestica.[J]Insect Biochemistry and Molecular Biology.2002,32:755-764.
314. Jennitte L Stevens et al.Inducible P450s of the CYP9 family from larval Manduca sexta midgut.[J]Insect Biochemistry and Molecular Biology.2000,30:559-568.
315. Xiaoning Liu et al.Induction of the cytochrome P450 activity by plant allelochemicals in the cotton bollworm, Helicoverpa armigera (Hubner).[J]Pesticide Biochemistry and Physiology.2006,84:127-134.
316. Xiao-ping Wang et al.Isolation and sequence analysis of a cDNA clone for a pyrethroid inducible cytochrome P450 from Helicoverpa armigera.[J]Insect Biochemistry and Molecular Biology.1995,9:1001-1009.
317. Xianchun Li et al.Molecular cloning and expression of CYP6B8—a xanthotoxin-inducible cytochrome P450 cDNA from Helicoverpa zea. [J]Insect Biochemistry and Molecular Biology. 2000,30:75-84.
318. Shinji Kasai et al. Molecular cloning, nucleotide sequence and gene expression of a cytochrome P450 (CYP6F1) from the pyrethroid-resistant mosquito, Culex quinquefasciatus Say.[J]Insect Biochemistry and Molecular Biology.2000,30:163-171.
319. Jeffrey G Scott.Cytochromes P450 and insecticide resistance.[J]Insect Biochemistry and Molecular Biology.1999,29:757-777.
320. Jeffrey G Scott et al.Cytochromes P450 of insects:the tip of the iceberg.[J]Pest Manag Sci. 2001,57:958-967.
321. Sung-Eun Lee et al.Biochemical mechanisms conferring cross-resistance to fumigant toxicities of essential oils in a chlorpyrifos-methyl resistant strain of Oryzaephilus surinamensis L.[J] Journal of Stored Products Research.2002,38:157-166.
322. Sung-Eun Lee et al.Cross-resistance of a chlorpyrifos-methyl resistant strain of Oryzaephilus surinamensis (Coleoptera Cucujidae) to fumigant toxicity of essential oil extracted from Eucalyptus globulus and its major monoterpene 1,8-cineole.[J]Journal of Stored Products Research.2000,36:383-389.
323. Helvig C et al.CYP15A1, the cytochrome P450 that catalyzes epoxidation of methyl farnesoate to juvenile hormone III in cockroach corpora allata.[J]PNAS.2004,101:4024-4029.
324. James T Warren et al.Molecular and biochemical characterization of two P450 enzymes in the ecdysteroidogenic pathway of Drosophila melanogaster.[J]PNAS.2002,99:11043-11048.
325. Kun Yan Zhu et al.Organophosphate Resistance Mediated by Alterations of Acetylcholinesterase in a Resistant Clone of the Greenbug, Schizaphis graminum.[J]Pesticide Biochemistry and Physiology.2000,68:138-147.
326. Craig E et al. Overview of Carboxylesterases and Their Role in the Metabolism of Insecticides.[J]J. Pestic, Sci.2005,30(2):75-83.
327. Takuo Fujisawa et al.Oxidative Activation and Degradation of Organophosphorus Pesticides Mediated by Iron Porphyrins.[J]Mndoj. Pestic. Sci.2005,30(2):103-110.
328. Papachristos DP et al.Selection of Acanthoscelides obtectus (Say) for resistance to lavender essential oil vapour.[J]Journal of Stored Products Research.2003,39:433-441.
329. Yurdagul Ferhatoglu et al.The basis for the safening of clomazone by phorate insecticide in cotton and inhibitors of cytochrome P450s.[J]Pesticide Biochemistry and Physiology.2005,81: 59-70.
330. Mbacke' Sembene.The origin of groundnut infestation by the seed beetle Caryedon serratus (Olivier).[J] Journal of Stored Products Research.2006,42:97-111.
331. Stephen G Sligar et al.Thirty years of microbial P450 monooxygenase research-Peroxo-heme intermediates—The central bus station in heme oxygenase catalysis.[J]iochemical and Biophysical Research Communications.2005,338:346-354.
332. Ranson H et al.Molecular analysis of multiple cytochrome P450 genes from the malaria vector,Anopheles gambiae.[J]Insect Molecular Biology.2002,11 (5):409-418.
333. Andrea L Crampton et al.A new family of cytochrome P450 genes (CYP41) from the cattle tick, Boophilus microplus.[J]Insect Biochemistry and Molecular Biology.1999,29:829-834.
334. Wen Z et al.Cloning of two novel P450 cDNAs in German cockroaches.[J]Insect Molecular Biology.2001,10(2):131-137.
335. Zhimou Wen et al.CYP9E2, CYP4C21 and related pseudogenes from German cockroaches Blattella germanica:implications for molecular evolution, expression studies and nomenclature of P450s.[J]Gene.2001,272:257-266.
336. Xianchun Li et al.Cytochrome P450 and actin genes expressed in Helicoverpa zea and Helicoverpa armigera-paralogy or orthology identification, gene conversion and evolution.[J] Insect Biochemistry and Molecular Biology.2002,32:311-320.
337. Bo Shen et al.Cytochrome P450 genes expressed in the deltamethrin-susceptible and-resistant strains of Culex pipiens pallens.[J]Pesticide Biochemistry and Physiology.2003,75:19-26.
338. Melissa C Hardstone et al.Deletion of Cyp6d4 does not alter toxicity of insecticides to Drosophila melanogaster.[J]Pesticide Biochemistry and Physiology.2006,84:236-242.
339. Chansak Suwanchaichinda et al.Effects of Exposure to Pesticides on Carbaryl Toxicity and Cytochrome P450 Activities in Aedes albopictus Larvae (Diptera-Culicidae).[J]Pesticide Biochemistry and Physiology.2001,70:63-73.
340. Markus Friedrich et al.Sequence and phylogenetic analysis of the complete mitochondrial genome of the flour beetle Tribolium castanaeum.[J]Molecular Phylogenetics and Evolution.2003,26:502-512.
341. Yoshiaki Fujita et al.Identification of multiple cytochrome P450 genes belonging to the CYP4 family in Xenopus laevis-cDNA cloning of CYP4F42 and CYP4V4. [J]Comparative Biochemistry and Physiology.2004,138:129-136.
342. Nannan Liu et al.Identification of two new cytochrome P450 genes and their 5'-flanking regions from the housefly, Musca domestica.[J]Insect Biochemistry and Molecular Biology.2002,32:755-764.
343. Kim F Rewitz et al.Identification, characterization and developmental expression of Halloween genes encoding P450 enzymes mediating ecdysone biosynthesis in the tobacco hornworm, Manduca sexta.[J]Insect Biochemistry and Molecular Biology.2006,36:188-199.
344. Charani Ranasinghe et al.Isolation and characterisation of a cytochrome b5 cDNA clone from Helicoverpa armigera (Hubner)-possible involvement of cytochrome b5 in cytochrome P450 CYP6B7 activity towards pyrethroids.[J]Insect Biochemistry and Molecular Biology.1999,29: 145-151.
345. Charani Ranasinghe et al.lsolation and characterization of two cytochrome P450 cDNA clones for CYP6B6 and CYP6B7 from Helicoverpa armigera (Hubner).[J]Insect Biochemistry and Molecular Biology.1998,28:571-580.
346. Li W et al.Molecular analysis of multiple CYP6B genes from polyphagous Papilio species.[J] Insect Biochemistry and Molecular Biology.2001,31:999-1011.
347. Ranson H.Molecular analysis of multiple cytochrome P450 genes from the malaria vector.[J] Insect Molecular Biology.2002,5:409-418.
348. Xianchun Li et al.Molecular cloning and expression of CYP6B8-a xanthotoxin-inducible cytochrome P450 cDNA from Helicoverpa zea.[J]Insect Biochemistry and Molecular Biology.2000,30:75-84.
349. He H et al.Molecular cloning and nucleotide sequence of a new P450 gene, CYP319A1, from the cattle tick, Boophilus microplus.[J]Insect Biochemistry and Molecular Biology.2002,32: 303-309.
350. Hongshan Li et al.Molecular cloning and nucleotide sequence of CYP6BF1 from the diamondback moth, Plutella xylostella.[J] Journal of Insect Science,2005,5(45):1-5.
351.Nanao Horike et al.Molecular cloning of NADPH-cytochrome P450 oxidoreductase from silkworm eggs-Its involvement in 20-hydroxyecdysone biosynthesis during embryonic development.[J]Eur. J. Biochem.2000,267:6914-6920.
352. Takashi Yamada et al.Molecular Cloning of Novel Cytochrome P450 Species Induced by Chemical Treatments in Cultured Tobacco Cells.[J]Pesticide Biochemistry and Physiology.2000, 68:11-25.
353. Shinji Kasai et al.Molecular cloning, nucleotide sequence and gene expression of acytochrome P450 (CYP6F1) from the pyrethroid-resistant mosquito, Culex quinquefasciatus Say.[J]Insect Biochemistry and Molecular Biology.2000,30:163-171.
354. Eriko Kamiya et al Molecular cloning, nucleotide sequences and gene expression of new cytochrome P450s (CYP6A24, CYP6D3v2) from the pyrethroid resistant housefly, Musca domestica L. (Diptera-Muscidae).[J] Appl. Entomol. Zool.2001,36 (2):225-229.
355. Martine MaRbe'che-Coisne et al.P450 and P450 reductase cDNAs from the moth Mamestra brassicae-cloning and expression patterns in male antennae.[J]Gene.2005,346:195-203.
356. Marcel Amichot et al.Point mutations associated with insecticide resistance in the Drosophila cytochrome P450 Cyp6a2 enable DDT metabolism.[J] Eur. J. Biochem.2004,271:1250-1257.
357. Hisashi S et al.Active-site characteristics of CYP2C19 and CYP2C9 probed with hydantoin and barbiturate inhibitors. [J] Archives of Biochemistry and Biophysics.2004,429:1-15.
358. Dimitra Nikou et al. An adult-specific CYP6 P450 gene is overexpressed in a pyrethroid-resistant strain of the malaria vector, Anopheles gambiae.[J]Gene.2003,318:91-102.
359. James C Fogleman et al.Analysis of fragment homology among DNA sequences from cytochrome P450 families 4 and 6.[J]Genetica.2001,110:257-265.
360. Yu SJ et al.Biochemical characteristics of insecticide resistance in the fall armyworm, Spodoptera frugiperda (J.E. Smith).[J] Pesticide Biochemistry and Physiology.2003,77:1-11.
361. Maibeche-Coisne M et al.cDNA cloning of biotransformation enzymes belonging to the cytochrome P450 family in the antennae of the noctuid moth Mamestra brassicae.[J]Insect Molecular Biology.2002,11(3):273-281.
362. Li W et al. CYP6B cytochrome P450 monooxygenases from Papilio canadensis and Papilio glaucus—potential contributions of sequence divergence to host plant associations.[J]Insect Molecular Biology.2002,11(6):543-551.
363. Sirkku T Saarikoski et al.Identification of genetic polymorphisms of CYP2S1 in a Finnish Caucasian population.[J]Mutation Research.2004,554:267-277.
364. Alexander Archakov et al.Inventory of the cytochrome P450 superfamily.[J]J Mol Model.2001, 7:140-142.
365. Masataka Sasabe et al.Molecular analysis of CYP321A1, a novel cytochrome P450 involved in metabolism of plant allelochemicals (furanocoumarins) and insecticides (cypermethrin) in Helicoverpa zea. [J]Gene.2004,338:163-175.
366. Daisuke Endo et al.Molecular cloning of P450 aromatase from the leopard gecko and its expression in the ovary. [J] Journal of Steroid Biochemistry & Molecular Biology.2005,96:131-140.
367. Julia W Pridgeon et al.Overexpression of CYP4G19 associated with a pyrethroid-resistant strain of the German cockroach, Blattella germanica (L.).[J]Gene.2003,314:157-163.
368. Martine MaRbe'che-Coisne et al.P450 and P450 reductase cDNAs from the moth Mamestra brassicae-cloning and expression patterns in male antennae.[J]Gene.2005,346:195-203.
369. April N Tucker et al. Polymorphisms in cytochrome P4503A5 (CYP3A5).[J]Cancer Letters.2005,217:61-72.
370. Tomita T et al.Molecular mechanisms involved in increased expression of a cytochrome P450 responsible for pyrethroid resistance in the housefly,Musca domestica.[J]Insect Molecular Biology.1995,4(3):135-140.
2. 陈冠荣主编.化工百科全书第4卷,发光材料一氟[M].北京：化学工业出版社.1993.
3.王娟韩薇.天然矿泉水中微量元素与人体健康[J].微量元素与健康研究.2001,18(2)：77-78.
4.侯振江周秀艳.微量元素与疾病[J].微量元素与健康研究.2004,21(6)：16-17.
5.孙殿军.中国地方病病情与防治进展[J].疾病控制杂志.2002,6(2)：97-100.
6. Ravichandran B, Roychowdhury A, Mukerjee A K, Gangopadhyay H N, Saiyed. Estimation of Fluoride Content in the Edible Vegetables of an Industrial Area in Orissa:Asian Journal of Water, Environment and Pollution,2007;2:93-95.
7. Guan ZZ, Xiao KQ, Zeng XY, Long YG, Cheng YH, Jiang SF, Wang YN.Changed cellular membrane lipid composition and lipid peroxidation of kidney in rats with chronic fluorosis: Arch Toxicol.2000,74:602-60.
8. World Health Organization (WHO). International Standards for Drinking Water. Geneva,2nd ed. 1963.
9. Jankovic S. In Manuel de chimie de l'environnement:OMS, Geneva 1974:251.
10. Hichour M, Persin F, Sandeaux J, Claude Gavach.Fluoride removal from waters by Donnan dialysis:Separation and Purification Technology.2000,18:1-11.
11. Mohapatra M, Anand S, Mishra BK, Giles Dion E, Singh P. Review of fluoride removal from drinking water:Journal of Environmental Management.2009:91:1-11.
12. Karthikeyan G, Shanmugasundarraj A. Isopleth mapping and In-Situ fluoride dependence on water quality in the Krishnagiri block of Tamil Nadu in south India:Fluoride.2000,33:121-7.
13.朱绯.高氟对斑马鱼造血基因Pul和cdx4的影响.[D]东北农业大学硕士学位论文.2007.
14.秦港.公鸡慢性氟中毒生殖毒性研究.[D]西南大学硕士学位论文.2006.
15.刘国艳.鸡氟中毒的毒理学研究.[D]东北农业大学博士学位论文.2000.
16.刘珊,吴天秀.氟化钠对大鼠血清微量元素的影响.[J]广东微量元素科学.2009,16(3)：22—26.
17.廖进民,陈艳.氟化物对幼年大鼠骨矿物质和血微量元素的影响机器相关性研究,2009,16(9)：13—19.
18.江琴.氟对雄性大鼠生殖细胞端粒酶表达影响的研究[D]郑州大学硕士学位论文.2005.
19.刘二龙.氟诱导原代大鼠肝细胞凋亡及bar基因表达研究[D]湖南农业大学硕士学位论文.2004.
20.贾丽红.氟致大鼠肝、肾细胞损伤机制及抗氧化营养素的干预性研究[D]中国医科大学博士学位论文.2002.
21.戴定戚,廖贤平.新生大鼠小肠上皮细胞分离培养研究[J].细胞生物学杂志.1997,19(1)：31-34.
22.陈思怀,刘燕.氟化物对培养猪小肠上皮细胞的影响[J].安徽农业科学.2008,36(3)：1062—1063，1098.
23.白焱,刘巍.水域氟污染与人体健康.[J]东北水利水电.2006,24(11)：268-269
24.朱其顺.中国地下水氟污染的现状及研究进展.[J]环境科学与管理.2009,34(1)：42-44
25.李凤华.地方性氟中毒对儿童智力发育的影响及氧化应激机制[D]南华大学硕士学位论文.2007
26.申秀英,章子贵,许晓路.氟对中枢神经系统的影响[J]中国地方病防治杂志.2001,16(6)：34
27.相有章,李明龙.现代慢性非传染性疾病预防与治疗[M]济南：山东科学技术出版社,2004：495.
28.邵红.地方性氟中毒患者骨骼损害及淋巴细胞基因表达的研究[D]新疆医科大学博士学位论文.2005.
29. Human Butyrylcholinesterase L330I Mutation Belongs to a Fluoride-Resistant Gene, by Expression in Human Fetal Kidney Cells. [J]Biochemical and Biophysical Research Communications 1997, (240):372-375.
30.张志民,任宝华,张家颖,高心,王成坤.变形链球菌耐氟菌株中耐酸相关基因dgk突变的检测及临床意义.[J]吉林大学学报：医学版.2008,34(5)：850-853.
31.姜葳,梁景平,李超伦,姜云涛,张明珠.远缘链球菌6715耐氟菌株与亲代菌株的基因表达差异.[J]上海交通大学学报：医学版,2007,27(2)：137-140.
32.林健荣,史奕山.氟素对家蚕幼虫血淋巴中酶活性及钙镁离子含量的影响.[J]蚕业科学.1994,20(2)：115-118.
33.沈海铭.桑蚕对氟化物的吸收排泄作用和抗氟性的关系.[J]绍兴文理学院学报.1997,17(6)：67-80.
34.沈海铭.桑蚕血液酸性磷酸酶活性和抗氟性关系的研究.[J]绍兴文理学院学报.1997,17(5)：51-54.
35.沈海铭.乙酰胆碱酯酶在桑蚕氟中毒中的表现以及和桑蚕抗氟性之间的关系.[J]绍兴文理学院学报.1998,18(6)：55-57.
36.陈志伟等.氟中毒家蚕过氧化氢酶活性的变化.[J]农村生态环境.2002,18(1)：31-34.
37.唐顺明,沈兴家等.家蚕雌雄性别耐氟性能差异的初步研究.[J]中国蚕业.2000(4)：16-17.
38. Chen YY,Wu YC.Effects of fluoride on changes of calcium,copper and ion level in the important organs of silkworm larvae(Bombyx mori).[J]Sericologia.1988,38(1):69-77.
39.王艳艳等.PCR-SSCP技术及其在植物研究中的应用.[J]生物信息学.2006,1：13-15.
40.唐顺明.蚕卵和氟中毒蚕体磷脂含量变化规律的研究[D]中国农业科学院硕士学位论文,2000.
41.杨明观,朱根生.以氟敏指数作度量指标的家蚕耐氟性测定法.[J]蚕业科学.1992,18(4)：227-231.
42.王章娥,吾布力艾合买提,刘明珠.家蚕耐氟性遗传与茧丝质性状相关性研究.[J]蚕桑通报.2005,36(1)：10-13.
43.刘伟强,林健荣,刘清神,陈芳艳,谢特新.人工饲料育蚕与桑叶育蚕对氟化物的抗性差异分析.[J]广东蚕业.2008,42(2)：16-18.
44.林昌麒,糜懿殿.家蚕耐氟显性主效基因的发现.[J]蚕业科学.1997,23(4)：237-239.
45.沈海铭.桑蚕抗氟性遗传模式的研究.[J]科技通报.1997,13(6)：419-422.
46.白会钗,徐安英,李木旺,赵云坡,郭秋红,钱荷英,张月华,赵远,黄勇平,郭锡杰.利用SSR标记对家蚕耐氟基因进行连锁定位分析.[J]蚕业科学.2008,34(2)：191-196.
47. Hongliang Zhou et al. Molecular cloning of Bombyx mori cytochrome P450 gene and its involvement in fluoride resistance.[J]Journal of Hazardous Materials.2008,779 (1):7-14.
48.周红亮,陈克平,姚勤,高路,陈慧卿,申红星,赵国力.家蚕bcl-2基因的克隆、序列分析及其表达模式.[J]农业生物技术学报.2008,16(3)：430-435.
49. Ranson H. Claudianos C, Ortelli F, Abgrall C, Hemingway J, Sharakhova M V, Unger M F, Collins FH and Feyereisen R. Evolution of supergene families associated with insecticide resistance. [J]Science (New York, N.Y).2002,298 (5591):179-181.
50. Claudianos C, Ranson H, Johnson RM, Biswas S, Schuler MA, Berenbaum MR, Feyereisen R and Oakeshott JG. A deficit of detoxification enzymes:pesticide sensitivity and environmental response in the honeybee. [J]Insect molecular biology.2006,15 (5):615-636.
51. Scott JG and Kasai S. Evolutionary plasticity of mono oxygenase-mediated resistance. Pesticide biochemistry and physiology. [J]2004,78 (3):171-178.
52. Scott JG, Liu N, Wen ZM. Insect cytoehromes P450:diversity insecticide resistance and tolerance to plant toxins.[J]Comp Biochem Physiol Part C,1998,121(1-3):147-155.
53. Scott JG,Zhimou Wen. Cytochromes P450 of insects:the tip of the iceberg.[J]PestManag Sci, 2001,57:958-967.
54.郭晶.乙酰胆碱酯酶的动力学机制及其应用[D]上海师范大学硕士学位论文.2007.
55. Feyereisen R. Insect P450 enzymes. Annual review of entomology.1999,44:507-533.
56.艾均文.家蚕(Bombyx mori)全基因组细胞色素P450基因结构与进化分析及CYP18A1的克隆与功能研究[D]西南大学博士学位论文.2007.
57. David R,Nelson. Metazoan cytochrome P450 evolution.[J]Comparative Biochemistry and Physiology,1998,121:15-22.
58. Feyereisen R.Evolution of insect P450.[J]Biochemical Society transactions.2006,34(6): 1252-1255.
59. Claudianos C, Ranson H, Johnson RM, Biswas S, Schuler MA, Berenbaum MR, Feyere-isen R and Oakeshott JG. A deficit of detoxification enzymes:pesticide sensitivity and en-vironmental response in the honeybee.[J]Insect molecular biology.2006,15(5):615-636.
60. Andersen JF, termoh len JGU et al. Expression of house fly CYP6A1 and NADPH-cytoc hrome P450 reductase in Escherichia coli and reconstitution of an insecticide metabolize-ng P450 system.[J]Biochemistry,1994,33 (8):2171-2177.
61. Tomita T, Scott JG. cDNA and deduced protein sequence of CYP6D1:the putative gene for a cytochrome P450 responsible for pyrethroid resistance in house fly. [J] Insect Bio-chem. Mol. Biol.1995(25):275-283.
62. Feyereisen R, Koener JF, Farnsworth DE,et al. Isolation and sequence of cDNA encodin g a cytochrome P450 from an insecticide-resistant strain of the house fly, Musca domest ica.[J]Proc Natl Acad Sci USA,1989(86):1465-1469.
63. Waters LC, Zelhof AC, Shaw BJ, et al. Possible involvement of the long terminal repe-at of transposable element 17.6 in regulating expression of an insecticide resistance-assoc iated P450 gene in Drosophila.[J] Proc. Natl. Acad. Sci. USA 1992 (89):4855-4859.
64. Cohen MB, Schuler MA, Berenbaum MR.A host-inducible cytochrome P450 from a host-specific caterpillar:molecular cloning and evolution.[J]Proc.Natl Acad.Sci.1992,90(12):564 3-5647
65.李斌,夏庆友,鲁成,等.家蚕细胞色素P450的基因组学分析.[J]中国科学C辑 生命科学2004,34(6)：517-521
66. Burgstalfler AW.Editorial:paradoxical dose-response effects of fluoride. Fluoride,2002,35:14 3-147.
67.刘清,杨克敌.氟化物与DNA损伤的研究进展[J].微量元素与健康研究,2003,20(6)：54—57.
68.谭红,张俊琴.氟中毒对免疫系统的影响[J].中华卫生监督与健康杂志,2002,1(2)：22—24.
69.张丹心,冯玉梅.氟诱发生殖激素失衡[J].国外医学,2004,25(3)：113—115.
70.许增禄,刘秉慈,缪庆.过量氟对大鼠骨骼I型胶原mRNA表达及纤维性状的影响.环境与健康杂志,2003,20(4)：204-206.
71.侯铁舟,李志丹.氟化物对体外器官培养人牙胚细胞增殖的相关调控蛋白表达的影响[J].西安交通大学学报,2003,24(4)：304—305.
72.王红,侯铁舟.氟化物作用下体外培养人牙胚内釉上细胞中Smad1、5表达的变化[J].口腔医学,2005,25(4)：201—204.
73.官志忠,王亚男.氟对SH-SY5Y神经细胞生物膜性脂质的作用[J].贵阳医学院学报,2005,30(1)：1—3.
74.闭立辉顾家栋胡乐山石美宁罗坚黄玲莉陆瑞好苏红梅韦博尤蒙艺英.家蚕抗氟品种“桂蚕F95”的选育研究[J].广西科学,2008,15(2)：198-204.
75.中华人民共和国国家标准牙膏GB8372-2001.
76.徐建峰许凌周卫龙.砖茶含氟量国家标准测定方法比较中国茶叶加工2007,(4)：47-49.
77. Leonard H Weinstein, Alan W Davison.Native plant species suitable as bioindicators and biomonitors for airborne fluoride. [J]Environmental Pollution 2003 125(1):3-11.
78.刘超,吴方正,傅柳松等.茶叶中的氟含量及测定方法研究[J].农业环境保护,1998,17(3)：132-135.
79.徐林生,顾晓山,翁荣林.桑树含氟量测定方法的讨论[J]江苏蚕业,1995,(3)：29-30.
80.徐建峰、周卫龙,茶叶中氟含量的测定.[J]中国茶叶加.2002(3)：35-36.
81.潘科,黄炳球.昆虫对有机磷类抗性机制研究进展.[J]世界农药,2001,23(2)：49-50.
82.郭晶.乙酰胆碱酯酶的动力学机制及其应用[D]上海师范大学硕士学位论文.2007.
83. Massoulie J, Pezzementi L, Bon S et al. Molecular and cellular biology of cholinesteras es. [J]Prog Neurobiol,1993,41:31
84. Taylor P, Radic Z Annu.The cholinesterases:from genes to proteins. [J]Rev Pharmacol T oxicol,1994,34:281
85. Rosenberry TL,Barnett P,Mays C.Acetylcholinesterase.[J]Areas Mol Biol,1982,43:103
86. Fallscheer HO, Cook JW, Assoc J. Studies on the conversion of some thionophosphates and a dithiophosphate to in vitro cholinesterase inhibitors. [J]Off Agr Chem,1956:692
87. Xianchun Li, Yinchang Wang.Progress in target-insensitive mechanism of insect resistance to insecticides. [J]Acta Entomologica Sinica.l998,41(4):417—425
88. Zhu KY,Lee SH,Clark JM.a point mutation of acetylcholinesterase associated with asinphosmethyl resistance and reduced fitness in Colorado potato betle. [J] Pest Biochem Physiol,1996,55:100—108.
89. Zhu KY,Clark JM.Validation of a point mutation of acetylcholinesterase in Colorado po-t ato beetle by polymerase chain reaction coupled to enzyme inhibition assay.[J]Pestle Bio chem Physiol,1997,57:28—35.
90. Fournier D,Bride JM,Hoffman F,et al.Acetylcholinesterase:two types ofmodifications confe r resistance to insecticide.[J] J Biol Chem,1992,267,14270—14274
91. Gao JR,Zhu KY.Comparative toxicity of selected organophasphate insecticides against resi stant and susceptible clones of the greenbug,Schizaphis graminum(Homoptera:aphididae).[J] J Agric Food Chem,2000,48:4717—4722.
92. Zhu KY,Gao JR.Increased activity associated with reduced sensitivity of acetylcholinester ase in organophosphate-resistant greenbug,Schizaphis graminum (Homoptera:Aphididae).[J] Pestic sci,1999,55:11—17.
93. Bradford MM. A rapid and sensitive method for the quantification of microgram quantiti es of protein utilizing the principle of protein dye binding.[J]Anal.B iochem.,1976,72:248.-254
94. Ellman GL, Courtny KD, Andres U.et al. A new and rapid colorimetric determination of acetylcholinesterase activity.[J]Biochem. Pharmacol.1961,(7):88—95
95. Swapnila Chouhana and SJS Flora. Effect of fluoride on the tissue oxidative stress and apoptosis in rats:Biochemical assays supported by IR spectroscopy data.[J]Tox-icology2008,254:61-67.
96. Eastbrook RW. The remarkerable P450s:A historical review of the versatile heme protein catalysis. [J]TASEB J,1996,10:202-204.
97. David R. Nelson. Metazoan cytochrome P450 evolution.[J]Comparative Biochemist-ry and Physiology,1998,121:15-22.
98. Feyereisen R. Evolution of insect P450.[J]Biochemical Society transactions.2006,34 (6):1252-1255.
99. Claudianos C, Ranson H, Johnson RM, Biswas S, Schuler MA, Berenbaum MR, Feyereisen R and Oakeshott JG. A deficit of detoxification enzymes:pesticide sensi-tivity and environmental response in the honeybee.[J]Insect molecular biology.2006, 15 (5):615-636.
100.Ranson H, Nikou D, Hutchinson M, Wang X, Roth CW, Hemingway J and Coll-ins FH. Molecular analysis of multiple cytochrome P450 genes from the malaria ve-ctor, Anopheles gambiae. [J]Insect molecular biology.2002,11 (5):409-418.
101.艾均文,董元凌,孔卫青等.细胞色素P450新家族的第一个基因CYP337A1的分子克隆与序列分析[J].西南大学学报(自然科学版),2008,30(2)：51-58.
102.吴明玮.白蚊伊蚊乙酰胆碱酯酶基因片段简并引物PCR、克隆及鉴定[D]暨南大学硕士学位论文.2001.
103.Daborn P, Boundy S, Yen J, Pittendrigh B, and ffrench-Constant R.DDT resistance in Drosophila correlates with Cyp6gl over-expression and confers cross-resistance to the ne onicotinoid imidacloprid[J]Mol Genet Genomics.2001,266(4):556-563.
104.尚金燕.家蚕两种类型乙酰胆碱酯酶基因的分析[D]浙江大学博士学位论文.2006.
105.张永亮.野桑蚕和桑尺蠖酚氧化酶的生化特性、基因克隆、表达及功能研究[D]西南大学博士学位论文.2007.
106.陈艳霞.家蝇乙酰胆碱酯酶基因的克隆、突变、表达与应用研究[D]浙江大学硕士学位论文.2007.
107.陈茂华.两种麦蚜乙酰胆碱酯酶的分子生物学研究[D]南京农业大学博士学位论文.2005.
108.施明安.果蝇和家蝇乙酰胆碱酯酶的表达与功能研究[D]中国科学院研究生院博士学位论文.2003.
109.方晓东.家蝇乙酰胆碱酯酶基因的克隆、表达及三维结构预测分析[D]华南热带农业大学硕士学位论文.2006.
110.彭霞.农药靶标乙酰胆碱酯酶的分离纯化及性质研究[D]四川大学硕士学位论文.2007.
111.柴玉鑫.嗜卷书虱乙酰胆碱酯酶生化毒理学及其基因克隆与序列分析[D]西南大学硕士学位论文.2007.
112.吴明玮等.白纹伊蚊乙酰胆碱酯酶基因片段克隆及鉴定.[J]中国寄生虫学与寄生虫病杂志；2001,19(4)：209-212.
113.吴明玮等.白纹伊蚊乙酰胆碱酯酶基因片段序列分析.[J]中国寄生虫学与寄生虫病杂志,2002,20(3)：187.
114.李春晓等.淡色库蚊乙酰胆碱酯酶全基因的克隆鉴定及蛋白同源模拟.[J]寄生虫与医学昆虫学报,2004,1l(3)：161-165.
115.丁中等.甘薯茎线虫乙酰胆碱酯酶基因ace-3全长cDNA的克隆和序列分析.[J]农业生物技术学报,2008,16(2)：326-331.
116.丁中等.甘薯茎线虫乙酰胆碱酯酶基因Dd-ace-2全长cDNA的克隆和序列分析.[J]生物工程学报,2008,25(2)：239-244.
117.林同等.橄榄星室木虱乙酰胆碱酯酶基因的克隆与序列分析.[J]吉林农业大学学报,2007,29(4)：368-370.
118.陈茂华等.禾谷缢管蚜、麦长管蚜乙酰胆碱酯酶基因片段的克隆与序列分析.[J]南京农业大学学报.2006,29(1)：35-39.
119.杨之帆等.褐飞虱乙酰胆碱酯酶基因全长cDNA的克隆及序列分析.[J]昆虫学报,2006,49(6)：1034-1041.
120.方晓东等.家蝇乙酰胆碱酯酶基因的克隆与序列分析.[J]中国热带医学.2006.,6(6)：59-62.
121.李昕等.家蝇中乙酰胆碱酯酶的提取纯化及应用研究.[J]分析试验室,2008,27(3)：87-90.
122.陶卉英等.嗜卷书虱抗性与敏感品系乙酰胆碱酯酶的比较.[J]西南农业大学学报,2005,27(2)：151-154.
123.王敦等.昆虫乙酰胆碱酯酶基因研究进展.[J]昆虫学报,2006,49(3)：497-503.
124.黄晓等.天然产物对家蚕乙酰胆碱酯酶抑制作用研究.[J]四川动物,200,27(3)：23-26.
125.丁中等.线虫乙酰胆碱酯酶研究进展.[J]植物保护.2008,.34(3)：81-84.
126.徐恩斌等.乙酰胆碱酯酶的研究进展.[J]国外医学·生理、病理科学与临床分册.2003,23(1)：73-75.
127.赵建庄等.乙酰胆碱酯酶分离纯化的方法.[J]北京农学院学报,2003,18(4)：249-251.
128.张明等.乙酰胆碱酯酶分子生物学研究进展.[J]农药.2006,45(1)：8-11.
129.魏福祥等.乙酰胆碱酯酶固定化方法的研究.[J]化学世界.2005,3：51-55.
130.刘铭钧等.乙酰胆碱酯酶基因工程技术研究进展.[J]生物技术.2008,18(2)：84-87.
131.张千等.乙酰胆碱酯酶生物功能的研究进展及其应用.[J]国外医学卫生学分册.2008,35(3)：143-146.
132.王小瑜等.乙酰胆碱酯酶试剂包配方的选择.[J]食品安全.2007,6：38-41.
133.奉水东等.PCR-SSCP检测肺癌EGFR基因突变.[J]现代检验医学杂志.2008,23(2)：13-15.
134.于成江等.常年性和季节性发情绵羊品种HIOMT基因的PCR-SSCP分析.[J]西北农林科技大学学报,2008,36(5)：31-35.
135.李春晓等.淡色库蚊乙酰胆碱酯酶基因上的可变剪接及对酶活性的影响.[J]寄生虫与医学昆虫学报,2007,14(3)：351-355.
136.颜鑫等.多作用点乙酰胆碱酯酶抑制剂研究进展.[J]中南药.2007,5(5)：397-401.
137.杨之帆等.褐飞虱乙酰胆碱酯酶基因全长cDNA的克隆及序列分析.[J]昆虫学报.2006,49(6)：1034-1041.
138.孙晓琴等.家蝇和果蝇的靶标抗性研究进展.[J]农药.2008,47(11)：785-789.
139.方晓东等.家蝇乙酰胆碱酯酶基因的克隆与序列分析.[J]中国热带医学.2006,6(6)：59-61.
140.李昕等.家蝇中乙酰胆碱酯酶的提取纯化及应用研究.[J]分析试验室.2008,27(3)：87-90.
141.李飞等.抗性品系棉蚜乙酰胆碱酯酶和羧酸酯酶的变异.[J]昆虫学报.2003,46(5)：578—583.
142.陶卉英等.嗜卷书虱抗性与敏感品系乙酰胆碱酯酶的比较.[J]西南农业大学学报,2005,27(2)：151-154.
143.潘科等.昆虫对有机磷类抗性机制研究进展.[J]世界农药.200123(2)：49-50.
144.曲明静等.昆虫乙酰胆碱酯酶基因变异抗药性机制研究.[J]昆虫知识.2007,44(2)：191-194.
145.王敦等.昆虫乙酰胆碱酯酶基因研究进展.[J]昆虫学报.2006,49(3)：497-503.
146.彭霞等.农药靶标乙酰胆碱酯酶的分离纯化及性质研究.[J]四川大学学报.2008,45(1)：90-95.
147.刘泽文等.羧酸酯酶和乙酰胆碱酯酶在褐飞虱对甲胺磷抗性发展中的作用.[J]农药学学报.2002,4(4)：51-55.
148.黄晓等.天然产物对家蚕乙酰胆碱酯酶抑制作用研究.[J]四川动.2008,27(3)：723-726.
149.吴明玮等.蚊乙酰胆碱酯酶基因突变与抗药性.[J]国外医学寄生虫病分册.2000,27(5)：201-204.
150.丁中等.线虫乙酰胆碱酯酶研究进展.[J]Plant Protection.2008,34(3):81-84.
151.王建军等.小菜蛾乙酰胆碱酯酶cDNA片段的克隆和序列分析.[J]昆虫学报.2002,45(4)：544-547.
152.张耀东等.乙酰胆碱酯酶的定点突变法设计及其在生物传感器领域的应用.[J]生物技术通报,2006,5：80-83.
153.郭晶等.乙酰胆碱酯酶的动力学机制及其应用.[J]农药,2007,46(1)：18-21.
154.徐恩斌等.乙酰胆碱酯酶的研究进展.[J]国外医学·生理、病理科学与临床分册,2003,23(1)：73-75
155.赵建庄等.乙酰胆碱酯酶分离纯化的方法.[J]北京农学院学报,2003,18(4)：249-251.
156.张明等.乙酰胆碱酯酶分子生物学研究进展.[J]农药,2006,45(1)：8-11.
157.魏福祥等.乙酰胆碱酯酶固定化方法的研究.[J]化学世界,2005,3：551-555.
158.刘铭钧等.乙酰胆碱酯酶基因工程技术研究进展.[J]生物技术..2008,18(2)：84-86.
159.张千等.乙酰胆碱酯酶生物功能的研究进展及其应用.[J]国外医学卫生学分册.2008,35(3)：143-147
160.刘芳等.乙酰胆碱酯酶性质改变与昆虫抗药性的关系.[J]华东昆虫学报2006.15(3)：187-191.
161.林建国等.与昆虫抗药性相关的乙酰胆碱酯酶基因突变研究进展.[J]农药学学报.2005,7(1)：01.06.
162.钟仰进等.氟化物引起家蚕的组织病变[J]华南农业大学学报.1994,15(1)：18-21.
163.王安皆,娄齐年,周丽霞,张凤林,聂磊,王娜.家蚕多丝量品种960l×92的耐氟性试验.[J]北方蚕业,2008,29(4)：18-19.
164.闭立辉,顾家栋,石美宁,罗坚,黄玲莉,苏红梅,韦博尤,蒙艺英.家蚕耐氟新品种“桂蚕F95"的主要性状.[J]广西蚕业,2008,45(2)：26-28.
165.郭定国,林健荣,黄嫔,林忠芬,钟苏苑，邱国祥,李林山,倪惠波,张桂玲,李惠华.家蚕新品种粤枫三号(五·选×28·32)的育成.[J]蚕业科学,2007,33(3)：466-469.
166.徐安英,林昌麒,侯成香,张月华,李木旺,孙平江,钱荷英.家蚕春用耐氟品种华源×东升的选育及其性状.[J]中国蚕业,2006,27(2)：30-32.
167.徐庆刚,陈克平,姚勤,陆健.家蚕耐氟基因RAPD分子标记的筛选及其克隆.[J]生物技术,2004,14(3)：10-11.
168.吾布力.艾合买提,王章娥,刘明珠.家蚕不同世代耐氟性比较及遗传分析_[J]中国蚕业,2004,25(3)：22-23.
169.赵巧玲,袭智勇,夏定国,章佩祯,潘新平,林方华,戴建一.耐氟蚕品种秋丰、白玉改良系的特点.[J]中国蚕业,2003,24(4)：54-55.
170.张升祥,王彦文,吴绪东,李春英,宋雪艳.山东家蚕现行品种耐氟性测定.[J]山东农业科学,2003(2)：39-40.
171.张士英,冯建琴,陈健康,沈建华,丁农,李玉峰,金卫明.家蚕春秋兼用耐氟杂交组合华·秋×松·白的选配.[J]蚕业科学,2003,29(3)：318-319.
172.姚耀涛,陈端豪.家蚕品种耐氟性比较.[J]中国蚕业,2002,23(3)：65-66.
173.沈建华.菁松F×皓月F的耐氟性测定及其茧丝质成绩比较.[J]中国蚕业,.2002,23(1)：27-29.
174.沈兴家,李奕仁等.春秋兼用耐氟蚕品种华峰GW×雪·A的选配.[J]蚕业科学,2002,28(1)：52-55.
175.姚勤,陈克平.家蚕分子标记辅助育种.[J]江苏蚕业,2002,24(3)：5-8.
176.张烈,鲁成等.陕西家蚕品种资源耐氟性调查初报.[J]西南农业大学学报.,2001,23(1)：26-28.
177.魏兆军,王章娥等.家蚕耐氟性状基因效应分析.[J]蚕业科学,2001,27(2)：158-161.
178.陈玉银.家蚕耐氟性差异的细胞化学研究.[J]昆虫学报,2000,43(3)：271-279.
179.林昌麒,陈克平.家蚕耐氟种质的创建.[J]蚕学通讯,2000,20(2)：1-7.
180.古巧珍,李亚清.若干家蚕基础品种的耐氟性测定.[J]北方蚕业,1999,20(1)：16-17.
181.王章娥,曹志清.家蚕品种间耐氟性比较及生化分析.[J]蚕业科学,1999,25(4)：258-260.
182.唐顺明,池兴家.蚕品种耐氟性能试验.[J]江苏蚕业,1998(1)：62-63.
183.林昌麒,糜懿殿.家蚕耐氟显性主效基因的发现.[J]蚕业科学,1997,23(4)：237-239.
184.林昌麒,糜懿殿.家蚕耐氟遗传初探.[J]江苏蚕业,1997,19(2)：7-10.
185.周金妹,杨明观.以氟敏指数为指标的家蚕耐氟性选择效果.[J]浙江农业大学学报,1996,22(4)：437-438.
186.刘晓勇,陈克平,姚勤,李军,蔡克亚.家蚕中肠组织抗核型多角体病毒病的相关蛋白分析.[J]昆虫学报,2008,51(4)：443-448.
187.蔡克亚,陈克平,刘晓勇,姚勤,李军.家蚕抗BmNPV品系与感性品系血淋巴液蛋白质组的差异分析.[J]生物工程学报,2008,24(2)：285-290.
188.赵国力,陈克平,姚勤,郭忠建.家蚕生殖细胞相关基因nanos的克隆表达及在胚胎发育中的表达模式.[J]昆虫学报,2007,50(11)：1092-1098.
189.高贵田,陈克平,姚勤,王林玲,陈慧卿.家蚕羧酸酯酶基因克隆及差异表达.[J]昆虫学报,2006,49(6)：930-937.
190.高贵田,陈克平,姚勤,徐家萍,王林玲,陈慧卿.家蚕eIF2a全长cDNA克隆及生物信息学分析.[J]中国农业科学.2006,39(5)：1031-1037.
191.陈克平,鲁成.分子连锁图与家蚕育种.[J]江苏蚕业,1999,21(3)：1-4.
192. Ivana Gazic et al. Preparative HPLC separation of bambuterol enantiomers and stereoselective inhibition of human cholinesterases.[J] Anal Bioanal Chem 2006.385:1513-1519.
193.任宝华.变形链球菌耐氟菌株中耐酸相关基因dgk和dltC突变的选择.[D]吉林大学硕士学位论文.2006
194.张磊.茶树氟吸收动力学特性的研究.[D]中国农业科学院硕士学位论文.2008
195.李加美.氟对体外培养血管内皮细胞功能的影响.[D]山东大学硕士学位论文.2007
196.仲伟丽.氟砷暴露对儿童智力水平及生长发育影响的调查分析.[D]山西医科大学硕士学位论文.2006
197.王锡林.氟致雄性大鼠生殖损害及硒对其拮抗作用.[D]郑州大学硕士学位论文.2002
198.梁大刚.氟中毒对家蚕幼虫体内谷胱甘肽(GSH)代谢的影响.[D]浙江大学硕士学位论文.2004
199.宁红梅.高氟低碘对老年大鼠不同脏器细胞DNA损伤的研究.[D]山西农业大学硕士学位论文.2004
200.向泉永.环境中氟对居民健康影响的危险度评价.[D]复旦大学博士学位论文.2004
201.李兴霞.鸡实验性慢性氟中毒致畸致变的研究.[D]西南农业大学硕士学位论文.2004
202.周红亮.家蚕cyp306al和bcl-2基因的鉴定及其耐氟性功能研究.[D]江苏大学硕士学位论文.2008
203.尚金燕.家蚕两种类型乙酰胆碱酯酶基因的分析.[D]浙江大学博士学位论文.2006
204.何琳.家蚕神经系统靶点基因克隆与功能研究.[D]中国科学院研究生院博士学位论文.2007
205.陈松等.6种增溶剂对棉铃虫细胞色素P450 O.脱甲基活性的影响.[J]南京农业大学学报2003,26(4)：127-129
206.韩雅莉等.疣荔枝螺微粒体细胞色素P450酶系的光谱分析.[J]光谱学与光谱分析.2006,26(1)：130-133.
207.曾晓等.德国小蠊抗性与敏感品系细胞色素p450生化特征的研究.[J]中国媒介生物学及控制杂志.2004,15(3)：175-179.
208.王爱红等.氯乙烯染毒对大鼠肝细胞色素P4502E1活力和mRNA表达的影响.[J]工业卫生与职业病.2005,31(3)：146-148.
209.郑明奇等.棉铃虫中肠微粒体p450的分离纯化.[J]昆虫学报2005,48(3)：359-364.
210.陈松等.不同种群棉铃虫三龄幼虫酯酶活性频率分布与抗药性的关系.[J]昆虫学报.2002,45(6)：733-738
211.胡小邦,朱昌亮.昆虫抗药性机制研究进展.[J]国外医学寄生虫病分册.2004,31(1)：18-23.
212_吴益东等.棉铃虫抗药性监测方法——浸叶法敏感毒力基线的建立及其应用.[J]昆虫学报,.2001,44(1)：56-61.
213.于彩虹,高希武,郑炳宗.2-十三烷酮对棉铃虫细胞色素p450的诱导作用.[J]昆虫学报.2002,45(1)：1-7.
214.马彩霞.棉铃虫细胞色素P450基因的克隆与表达[D]西北农林科技大学博士学位论文.2005
215.黄剑.小菜蛾抗阿维菌素品系细胞色素P450的研究.[D]西北农林科技大学博士学位论文.2005
216.陈松.与氰戊菊酯抗性相关的棉铃虫细胞色素P450基因的克隆及异源表达.[D]南京农业大学博士学位论文.2005
217.陈秋霞等.昆虫细胞色素P450的诱导和抗药性.[J]热带医学杂志2001,1(1)：67-70.
218.邱星辉.昆虫细胞色素P450研究的一些新进展.[J]昆虫知识,2003.40(6)：487-491.
219.张听等.昆虫细胞色素P450与抗药性关系的分子生物学研究进展.[J]中国寄生虫病防治杂志,2002,15(1)：62-64.
220.于彩虹等.棉铃虫细胞色素P450CO差光谱的测定.[J]昆虫学报.2005,48(2)：301—304.
221.郑明奇等.棉铃虫中肠微粒体P450的分离纯化.[J]昆虫学报.2005,48(3)：359—364.
222.张永强等.嗜卷书虱抗气性和抗药性品系呼吸代谢的比较.[J]西南农业大学学报,2003,25(5)：413-416.
223.曹俊等.嗜人按蚊溴氰菊酯抗性分子机理研究：Ⅲ嗜人按蚊P450基因mRNA荧光半定量RT-PCR分析.[J]中国血吸虫病防治杂志.2005,17(6)：433-436.
224.郑明奇等.细胞色素P450s酶系催化除草剂代谢的研究进展[J]农药学学报.2003,5(3)：1-8.
225.赵博等.细胞色素P450的研究进展.[J]山东农业大学学报(自然科学版),2004,35(I)：142-144.
226.吕敏等.细胞色素P450酶系与苦皮藤素V对昆虫选择毒性的关系研究.[J]西北农林科技大学学报,2003,31(3)：35-38.
227.朱大岭等.细胞色素P450酶系在药物代谢中的作用.[J]医药导报2004.,23(7)：440-443.
228.陈玉华等.细胞色素P450在家蚕中的研究.[J]江苏蚕业,2005,2：1-3
229.刘小宁等.应用毛细管气相色谱法检测棉铃虫细胞色素P450 O-脱甲基活性.[J]昆虫知识.2004,41(3)：232-235.
230.周国理等.昆虫CYP6家族多样性与进化.[J]昆虫知识，2002，39(4)：246-251.
231.邱星辉等.昆虫CYP6家族多样性与进化.[J]昆虫知识,2003,40(6)：487-491.
232.陈婕等.细胞色素P450氧化还原酶的研究进展.[J]中国药理学通报.2006,22(2)：129-133.
233.刘德立等.细胞色素P450与农药相互作用及其机理研究.[J]华中师范大学学报,2005,39(4)：518-523.
234.邱星辉等.细胞色素P450在植物与昆虫相互关系中的作用.[J]昆虫知识.2002,39(1)：5-8.
235.周国理等.白纹伊蚊CYP6N3基因启动子序列的分子克隆与功能分析.[J]寄生虫与医学昆虫 学报,2002,9(1)：12-20.
236.吴瑜等.白纹伊蚊基因组中CYP6家族新成员基因片段的克隆与分析.[J]寄生虫与医学昆虫学报.2001,8(1)；23-30.
237.周国理等.白纹伊蚊溴氰菊酯抗性株基因组中CYP6基因片段的分离与鉴定.[J]中国人兽共患病杂志.2000,16(1)：22-26.
238.王立新等.草鱼MyoD cDNA的克隆和序列分析.[J]中国农业科学.2005,38(10)：2134-2138.
239.肖勇梅等.巢式PCR法构建人细胞色素P4501A1 基因 cDNA全长T载体.[J]广东医学,2005,26(8)：1046-1049.
240.腾达等.淡色库蚊抗溴氰菊酯品系CYP4家族新成员基因克隆及序列分析.[J]中国寄生虫病防治杂志.2004,17(2)：65-68.
241.李秀兰等.淡色库蚊细胞色素P450 CYP4E2r6基因的克隆、表达及鉴定.[J]中国人兽共患病杂志.2005,21(7)：552-558.
242.朱昌亮等.淡色库蚊细胞色素p450基因研究.[J]生物化学与生物物理进展.2001：28(1)：61-67.
243.申望等.腐生型眼虫Astasia longa两种TIM同工酶cDNA克隆分析.[J]动物学报.2004,50(3)：414-419.
244.朱礼华等.家蝇抗性相关p450基因的初步研究.[J]寄生虫与医学昆虫学报,2000,7(2)：99-103.
245.艾颖等.棉铃虫细胞色素P450 cDNA片段的克隆与序列分析.[J]昆虫知识,200441(3)：227-232.
246.黄炯烈等.蚊虫细胞色素p450基因系列研究.[J]中山医科大学学报.2001.,22(6)：401-405,422.
247.李洪山等.小菜蛾细胞色素P450基因的cDNA片段克隆及其序列分析.[J]江苏农业学报,2006,22(1)：29-32.
248.王东兰等.紫茎泽兰CYP75基因cDNA片段的克隆与鉴定.[J]植物保护.2005,31(4)：65-70.
249. Guo chong Zhi et al. Molecular simulation of a single-chain antibody against AChE to explore molecular basis of inhibitory effect of 3F3M cAb on enzyme activity.[J]Acta Pharmacol Sin 2003,24(5):460-466.
250. Nannan Liu et al. CYP4AB1, CYP4AB2, and Gp-9 gene overexpression associated with workers of the red imported fire ant, Solenopsis invicta Buren.[J]Gene.2004,327:81-87.
251. Nannan Liu et al. Differential expression of genes in pyrethroid resistant and susceptible mosquitoes, Culex quinquefasciatus (S.).[J] Gene.2007,394:61-68.
252. Nannan Liu et al. Identification of two new cytochrome P450 genes and their 5_flanking regions from the housefly, Musca domestica. [J] Insect Biochemistry and Molecular Biology.2002,32:755-764.
253. Qiang Xu et al. Kdr allelic variation in pyrethroid resistant mosquitoes, Culex quinquefasciatus (S.).[J] Biochemical and Biophysical Research Communications.2006,345:774-780.
254. Julia W. Pridgeon, Nannan Liu.Overexpression of the cytochrome c oxidase subunit I gene associated with a pyrethroid resistant strain of German cockroaches, Blattella germanica (L.)[J] Insect Biochemistry and Molecular Biology.2003,33:1043-1048.
255. Qiang Xu et al.Sodium channel gene expression associated with pyrethroid resistant house f lies and German cockroaches.[J] Gene.2006,379:62-67.
256. Helvig C et al.CYP15A1, the cytochrome P450 that catalyzes epoxidation of methyl farnesoate to juvenile hormone Ⅲ in cockroach corpora allata.[J] PNAS.2004,101(12):4024-4029.
257. Claudianos C et al. A deficit of detoxification enzymes:pesticide sensitivity and environmental
response in the honeybee[J]Insect Molecular Biology.2006,15 (5):615-636.
258. Srividya Bhaskara et al. Caffeine induction of Cyp6a2 and Cyp6a8 genes of Drosophila melanogaster is modulated by cAMP and D-JUN protein levels.[J] Gene.2008,415:49-59.
259. Cynthia J Coffman et al. Identification of coregulated transcripts affecting male body size in Drosophila.[J]Genome Biology.2005,6:53.
260. Hongshan Li et al. Molecular cloning and nucleotide sequence of CYP6BF1 from the diamondback moth, Plutella xylostella[J] JIS.2005,5:45.
261. Sutherland TD et al. A cytochrome P450 terpenoid hydroxylase linked to the suppression of insect juvenile hormone synthesis.[J] Proc. Nati. Acad. Sci.1998,95:12884-12889.
262. Michael B.Cohen et al. A Host-Inducible Cytochrome P450 from a Host-Specific Caterpillar-Molecular Cloning and Evolution.[J] Proc.Nati.Acad.Sci.1992,89:10920-10924.
263. Michael R. Bogwitz, Henry Chung, Lorin Magoc et al.Cypl2a4 confers lufenuron resistance in a natural population of Drosophila melanogaster. [J] PNAS.2005,102(36):12807-12812.
264. Thomas L. Poulos Cytochrome P450 flexibility.[J] PNAS.2003,100 (23):13121-13122.
265. Mudgapallia shok et al. Insect juvenile hormone resistance gene homology with the bHLH-PAS family of transcriptional regulators.[J]Proc.Natl.Acad.Sci.1998,95:2761-2766.
266. Thomas G. Wilson et al. Insecticide resistance resulting from an absence of target-site gene product.[J] Proc.Natl.Acad.Sci.1998,95:14040-14044.
267. Reni Feyereisen et al. Isolation and Sequence of cDNA Encoding a Cytochrome P-450 from an Insecticide-Resistant Strain of the House Fly, Musca domestica. [J]Proc.Natl.Acad.Sci. 1989,86:1465-1469.
268. Tetsuro Shinoda et al. Juvenile hormone acid methyltransferase--A key regulatory enzyme for insect metamorphosis.[J] PNAS.2003,100(21):11986-11991.
269. James T. Warren et al. Molecular and biochemical characterization of two P450 enzymes in the ecdysteroidogenic pathway of Drosophila melanogaster.[J] PNAS.2002,99(17):11043-11048.
270. Phillip B, Danielson et al. Molecular cloning of a family of xenobiotic-inducible drosophilid cytochrome P450s-Evidence for involvement in host-plant allelochemical resistance.[J] Proc. Natl. Acad.Sci.1997,94:10797-10802.
271. Stefan Jennewein et al. Random sequencing of an induced Taxus cell cDNA library for identification of clones involved in Taxol biosynthesis. [J] PNAS.2004,101(24):9149-9154.
272. Anna Petryk et al. Shade is the Drosophila P450 enzyme that mediates the hydroxylation of ecdysone to the steroid insect molting hormone 20-hydroxyecdysone.[J] PNAS.2003,25(100):13773-13778.
273. Xianchun Li et al.Structural and functional divergence of insect CYP6B proteins-From specialist to generalist cytochrome P450.[J] PNAS.2004,101(9):2939-2944.
274. Jean-Philippe David et al. The Anopheles gambiae detoxification chip-A highly specific microarray to study metabolic-based insecticide resistance in malaria vectors.[J]PNAS.2005, 102(11):4080-4084.
275. Randy L,Rose et al. Cytochrome P450-Associated Insectici de Resistance and the Development of Biochemical Diagnostic Assays in Heliothis virescens.[J]Pesticide Biochemistry and Physiology.1995,51:178-191.
276. Yu SJ et al.Biochemical characteristics of insecticide resistance in the fall armyworm, Spodoptera frugiperda (J.E. Smith).[J]Pesticide Biochemistry and Physiology.2003,77:1-11.
277. Renaud Le Bouquin et al.CYP94A5, a new cytochrome P450 from Nicotiana tabacum is able to catalyze the oxidation of fatty acids to the-alcohol and to the corresponding diacid.[J]Eur. J. Biochem.2001,268:3083-3090.
278. Min-SooYun et al.Cytochrome P-450 monooxygenase activity in herbicide-resistant and-susceptible late watergrass (Echinochloa phyllopogon).[J]Pesticide Biochemistry and Physiology.2005,83:107-114.
279. Christiane N,Gouamene-Lamine et al.Differential susceptibility to abamectin and two bioactive avermectin analogs in abamectin-resistant and susceptible strains of Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera:Chrysomelidae).[J]Pesticide Biochemistry and Physiology.2003,76:15-23.
280. Chansak Suwanchaichinda et al.Effects of Exposure to Pesticides on Carbaryl Toxicity and Cytochrome P450 Activities in Aedes albopictus Larvae.[J]Pesticide Biochemistry and Physiology.2001,70:63-73.
281. Sivapiriya V et al. Evects of dimethoate (0,0-dimethyl S-methyl carbamoyl methyl phosphorodithioate) and Ethanol in antioxidant status of liver and kidney of experimental mice.[J] Pesticide Biochemistry and Physiology.2006,85:115-121.
282. Anita Ma, Bautista M et al.Identification of permethrin-inducible cytochrome P450s from the diamondback moth, Plutella xylostella (L.) and the possibility of involvement in permethrin resistance.[J]Pesticide Biochemistry and Physiology.2006,85:1-9.
283. Emmanouil Roditakis et al.IdentiWcation of mutations in the para sodium channel of Bemisia tabaci from Crete, associated with resistance to pyrethroids.[J]Pesticide Biochemistry and Phy siology.2006,85:161-166.
284. David JP et al.Involvement of cytochrome P450 monooxygenases in the response of mosquito larvae to dietary plant xenobiotics.[J]Insect Biochemistry and Molecular Biology.2006,36:410-420.
285. Tsagkarakou A et al. Mechanisms of resistance to organophosphates in Tetranychus urticae (Acari-Tetranychidae) from Greece. [J]Insect Biochemistry and Molecular Biology.2002,32:417-424.
286. Si-Woo Lee.Metabolic resistance mechanisms of the housefly(Musca domestica) resistant to pyraclofos.[J]Pesticide Biochemistry and Physiology.2006,85:76-83.
287. Rachel L. Cox et al.Nitric oxide synthase sequences in the marine fish Stenotomus chrysops and the sea urchin Arbacia punctulata, and phylogenetic analysis of nitric oxide synthase calmodulin-binding domains.[J]Comparative Biochemistry and Physiology.2001,130:479-491.
288. Tomoko Takeda et al.Parasitization by Cotesia plutellae enhances detoxifying enzyme activity in Plutella xylostella.[J] Pesticide Biochemistry and Physiology.2006,86:15-22.
289. Katsuya Natsuhara et al.Phenobarbital induction of permethrin detoxification and phenobarbital metabolism in susceptible and resistant strains of the beet armyworm Spodoptera exigua (Hiibner).[J] Pesticide Biochemistry and Physiology.2004,79:33-41.
290. Yang Y et al.The involvement of microsomal oxidases in pyrethroid resistance in Helicoverpa armigera from Asia.[J]Insect Biochemistry and Molecular Biology.2004,34:763-773.
291. Julia W. Pridgeon et al.Variability of resistance mechanisms in pyrthroid resistant German cockroaches.[J]Pesticide Biochemistry and Physiology.2002,73:149-156.
292. Jeffrey G. Scott.Cytochromes P450 and insecticide resistance.[J]Insect Biochemistry and Molecular Biology.1999,29:757-777.
293. Chansak Suwanchaichinda et al. Effects of Exposure to Pesticides on Carbaryl Toxicity and Cytochrome P450 Activities in Aedes albopictus Larvae.[J]Pesticide Biochemistry and Physiology.2001,70:63-73.
294. Craig Sams et al. Evidence for the activation of organophosphate pesticides by cytochromes P450 3A4 and 2D6 in human liver microsomes.[J]Toxicology Letters.2000,116:217-221.
295. Si-Woo Lee et al.Metabolic resistance mechanisms of the housefly (Musca domestica) resistant to pyraclofos.[J]Pesticide Biochemistry and Physiology.2006,85:76-83.
296. Yang Y et al.The involvement of microsomal oxidases in pyrethroid resistance in Helicoverpa armigera from Asia.[J]Insect Biochemistry and Molecular Biology.2004,34:763-773.
297. Janet Hemingway et al.The molecular basis of insecticide resistance in mosquitoes.[J]Insect Biochemistry and Molecular Biology.2004,34:653-665.
298. Scharf ME et al. Expression and induction of three family 4 cytochrome P450 genes identified from insecticide resistant and susceptible western corn rootworms Diabrotica virgifera virgifera.[J] Insect Molecular Biology.2001,10(2):139-146.
299. Festucci-Buselli RA et al.Expression of Cyp6g1 and Cyp12d1 in DDT resistant and susceptible strains of Drosophila melanogaster.[J]Insect Molecular Biology.2005,14(1):69-77.
300. Shinji Kasai et al.Molecular cloning, nucleotide sequence and gene expression of a cytochrome P450 (CYP6F1) from the pyrethroid-resistant mosquito, Culex quinquefasciatus Say.[J]Isect Biochemistry and Molecular Biology.2000,30:163-171.
301. Shinji Kasai et al. Male specific expression of a cytochrome P450 (Cyp312al) in Drosophila melangaster.[J]Biochemical and Biophysical Research Communications.2003,300:894-900.
302. Shinji Kasai et al. Evolutionary plasticity of monooxygenase-mediated resistance[J] Pesticide Biochemistry and Physiology.2004,78:171-178.
303. Shinji Kasai et al.P450 Monooxygenases Are an Important Mechanism of Permethrin Resistance in Culex uinquefasciatus Say Larvae.[J]Archives of Insect Biochemistry and Physiology.1998,37:47-56.
304. Jeffrey G. Scott et al House-fly cytochrome P450 CYP6D1:5'flanking sequences and comparison of alleles.[J]Gene.1999,226:347-353.
305. Shinji Kasai et al.Overexpression of Cytochrome P450 CYP6D1 Is Associated with Monooxygenase-Mediated Pyrethroid Resistance in House Flies from Georgia.[J]Pesticide Biochemistry and Physiology.2000,68:34-41.
306. Shinji Kasai et al. A House Fly Gene Homologous to the Zinc Finger Proto-oncogene Gfi-1.[J] Biochemical and Biophysical Research Communications.2001,283:644-647.
307. Kasai S et al. Molecular cloning and nucleotide sequence of a cytochrome P450 cDNAfrom a pyrethroid-resistant mosquito,Culex quinquefasciatus Say.[J]Insect Molecular Biology.1998,30: 185-190.
308. Li Zhang et al.In Vitro Metabolism of Pyriproxyfen by Microsomes FromSusceptible and Resistant Housefly Larvae.[J] Archives of Insect Biochemistry and Physiology.1998,37:215-224
309. Toshio Shono et al.Genetics and mechanisms of permethrin resistance in the YPER strain of house fly.[J]Pesticide Biochemistry and Physiology.2002,73:27-36.
310. Kelsey A.Milleret alAssessment of cytochrome P450 1A in harbour seals (Phoca vitulina) using a minimally-invasive biopsy approach.[J]Marine Environmental Research.2005,60:153-169.
311. Randy Rose et al.Cytochrome P450 (CYP)9A1 in Heliothis virescens-the first member of a new CYP family.[J]Insect Biochemistry.1997,279(6):605-615.
312. Omprakash Mittapalli et al.Differential expression of two cytochrome P450 genes in compatible and incompatible Hessian fly-wheat interactions. [J]Insect Biochemistry and Molecular B iology.2005,35:981-989.
313.Nannan Liu et al.Identification of two new cytochrome P450 genes and their 5'-flanking regions from the housefly, Musca domestica.[J]Insect Biochemistry and Molecular Biology.2002,32:755-764.
314. Jennitte L Stevens et al.Inducible P450s of the CYP9 family from larval Manduca sexta midgut.[J]Insect Biochemistry and Molecular Biology.2000,30:559-568.
315. Xiaoning Liu et al.Induction of the cytochrome P450 activity by plant allelochemicals in the cotton bollworm, Helicoverpa armigera (Hubner).[J]Pesticide Biochemistry and Physiology.2006,84:127-134.
316. Xiao-ping Wang et al.Isolation and sequence analysis of a cDNA clone for a pyrethroid inducible cytochrome P450 from Helicoverpa armigera.[J]Insect Biochemistry and Molecular Biology.1995,9:1001-1009.
317. Xianchun Li et al.Molecular cloning and expression of CYP6B8—a xanthotoxin-inducible cytochrome P450 cDNA from Helicoverpa zea. [J]Insect Biochemistry and Molecular Biology. 2000,30:75-84.
318. Shinji Kasai et al. Molecular cloning, nucleotide sequence and gene expression of a cytochrome P450 (CYP6F1) from the pyrethroid-resistant mosquito, Culex quinquefasciatus Say.[J]Insect Biochemistry and Molecular Biology.2000,30:163-171.
319. Jeffrey G Scott.Cytochromes P450 and insecticide resistance.[J]Insect Biochemistry and Molecular Biology.1999,29:757-777.
320. Jeffrey G Scott et al.Cytochromes P450 of insects:the tip of the iceberg.[J]Pest Manag Sci. 2001,57:958-967.
321. Sung-Eun Lee et al.Biochemical mechanisms conferring cross-resistance to fumigant toxicities of essential oils in a chlorpyrifos-methyl resistant strain of Oryzaephilus surinamensis L.[J] Journal of Stored Products Research.2002,38:157-166.
322. Sung-Eun Lee et al.Cross-resistance of a chlorpyrifos-methyl resistant strain of Oryzaephilus surinamensis (Coleoptera Cucujidae) to fumigant toxicity of essential oil extracted from Eucalyptus globulus and its major monoterpene 1,8-cineole.[J]Journal of Stored Products Research.2000,36:383-389.
323. Helvig C et al.CYP15A1, the cytochrome P450 that catalyzes epoxidation of methyl farnesoate to juvenile hormone III in cockroach corpora allata.[J]PNAS.2004,101:4024-4029.
324. James T Warren et al.Molecular and biochemical characterization of two P450 enzymes in the ecdysteroidogenic pathway of Drosophila melanogaster.[J]PNAS.2002,99:11043-11048.
325. Kun Yan Zhu et al.Organophosphate Resistance Mediated by Alterations of Acetylcholinesterase in a Resistant Clone of the Greenbug, Schizaphis graminum.[J]Pesticide Biochemistry and Physiology.2000,68:138-147.
326. Craig E et al. Overview of Carboxylesterases and Their Role in the Metabolism of Insecticides.[J]J. Pestic, Sci.2005,30(2):75-83.
327. Takuo Fujisawa et al.Oxidative Activation and Degradation of Organophosphorus Pesticides Mediated by Iron Porphyrins.[J]Mndoj. Pestic. Sci.2005,30(2):103-110.
328. Papachristos DP et al.Selection of Acanthoscelides obtectus (Say) for resistance to lavender essential oil vapour.[J]Journal of Stored Products Research.2003,39:433-441.
329. Yurdagul Ferhatoglu et al.The basis for the safening of clomazone by phorate insecticide in cotton and inhibitors of cytochrome P450s.[J]Pesticide Biochemistry and Physiology.2005,81: 59-70.
330. Mbacke' Sembene.The origin of groundnut infestation by the seed beetle Caryedon serratus (Olivier).[J] Journal of Stored Products Research.2006,42:97-111.
331. Stephen G Sligar et al.Thirty years of microbial P450 monooxygenase research-Peroxo-heme intermediates—The central bus station in heme oxygenase catalysis.[J]iochemical and Biophysical Research Communications.2005,338:346-354.
332. Ranson H et al.Molecular analysis of multiple cytochrome P450 genes from the malaria vector,Anopheles gambiae.[J]Insect Molecular Biology.2002,11 (5):409-418.
333. Andrea L Crampton et al.A new family of cytochrome P450 genes (CYP41) from the cattle tick, Boophilus microplus.[J]Insect Biochemistry and Molecular Biology.1999,29:829-834.
334. Wen Z et al.Cloning of two novel P450 cDNAs in German cockroaches.[J]Insect Molecular Biology.2001,10(2):131-137.
335. Zhimou Wen et al.CYP9E2, CYP4C21 and related pseudogenes from German cockroaches Blattella germanica:implications for molecular evolution, expression studies and nomenclature of P450s.[J]Gene.2001,272:257-266.
336. Xianchun Li et al.Cytochrome P450 and actin genes expressed in Helicoverpa zea and Helicoverpa armigera-paralogy or orthology identification, gene conversion and evolution.[J] Insect Biochemistry and Molecular Biology.2002,32:311-320.
337. Bo Shen et al.Cytochrome P450 genes expressed in the deltamethrin-susceptible and-resistant strains of Culex pipiens pallens.[J]Pesticide Biochemistry and Physiology.2003,75:19-26.
338. Melissa C Hardstone et al.Deletion of Cyp6d4 does not alter toxicity of insecticides to Drosophila melanogaster.[J]Pesticide Biochemistry and Physiology.2006,84:236-242.
339. Chansak Suwanchaichinda et al.Effects of Exposure to Pesticides on Carbaryl Toxicity and Cytochrome P450 Activities in Aedes albopictus Larvae (Diptera-Culicidae).[J]Pesticide Biochemistry and Physiology.2001,70:63-73.
340. Markus Friedrich et al.Sequence and phylogenetic analysis of the complete mitochondrial genome of the flour beetle Tribolium castanaeum.[J]Molecular Phylogenetics and Evolution.2003,26:502-512.
341. Yoshiaki Fujita et al.Identification of multiple cytochrome P450 genes belonging to the CYP4 family in Xenopus laevis-cDNA cloning of CYP4F42 and CYP4V4. [J]Comparative Biochemistry and Physiology.2004,138:129-136.
342. Nannan Liu et al.Identification of two new cytochrome P450 genes and their 5'-flanking regions from the housefly, Musca domestica.[J]Insect Biochemistry and Molecular Biology.2002,32:755-764.
343. Kim F Rewitz et al.Identification, characterization and developmental expression of Halloween genes encoding P450 enzymes mediating ecdysone biosynthesis in the tobacco hornworm, Manduca sexta.[J]Insect Biochemistry and Molecular Biology.2006,36:188-199.
344. Charani Ranasinghe et al.Isolation and characterisation of a cytochrome b5 cDNA clone from Helicoverpa armigera (Hubner)-possible involvement of cytochrome b5 in cytochrome P450 CYP6B7 activity towards pyrethroids.[J]Insect Biochemistry and Molecular Biology.1999,29: 145-151.
345. Charani Ranasinghe et al.lsolation and characterization of two cytochrome P450 cDNA clones for CYP6B6 and CYP6B7 from Helicoverpa armigera (Hubner).[J]Insect Biochemistry and Molecular Biology.1998,28:571-580.
346. Li W et al.Molecular analysis of multiple CYP6B genes from polyphagous Papilio species.[J] Insect Biochemistry and Molecular Biology.2001,31:999-1011.
347. Ranson H.Molecular analysis of multiple cytochrome P450 genes from the malaria vector.[J] Insect Molecular Biology.2002,5:409-418.
348. Xianchun Li et al.Molecular cloning and expression of CYP6B8-a xanthotoxin-inducible cytochrome P450 cDNA from Helicoverpa zea.[J]Insect Biochemistry and Molecular Biology.2000,30:75-84.
349. He H et al.Molecular cloning and nucleotide sequence of a new P450 gene, CYP319A1, from the cattle tick, Boophilus microplus.[J]Insect Biochemistry and Molecular Biology.2002,32: 303-309.
350. Hongshan Li et al.Molecular cloning and nucleotide sequence of CYP6BF1 from the diamondback moth, Plutella xylostella.[J] Journal of Insect Science,2005,5(45):1-5.
351.Nanao Horike et al.Molecular cloning of NADPH-cytochrome P450 oxidoreductase from silkworm eggs-Its involvement in 20-hydroxyecdysone biosynthesis during embryonic development.[J]Eur. J. Biochem.2000,267:6914-6920.
352. Takashi Yamada et al.Molecular Cloning of Novel Cytochrome P450 Species Induced by Chemical Treatments in Cultured Tobacco Cells.[J]Pesticide Biochemistry and Physiology.2000, 68:11-25.
353. Shinji Kasai et al.Molecular cloning, nucleotide sequence and gene expression of acytochrome P450 (CYP6F1) from the pyrethroid-resistant mosquito, Culex quinquefasciatus Say.[J]Insect Biochemistry and Molecular Biology.2000,30:163-171.
354. Eriko Kamiya et al Molecular cloning, nucleotide sequences and gene expression of new cytochrome P450s (CYP6A24, CYP6D3v2) from the pyrethroid resistant housefly, Musca domestica L. (Diptera-Muscidae).[J] Appl. Entomol. Zool.2001,36 (2):225-229.
355. Martine MaRbe'che-Coisne et al.P450 and P450 reductase cDNAs from the moth Mamestra brassicae-cloning and expression patterns in male antennae.[J]Gene.2005,346:195-203.
356. Marcel Amichot et al.Point mutations associated with insecticide resistance in the Drosophila cytochrome P450 Cyp6a2 enable DDT metabolism.[J] Eur. J. Biochem.2004,271:1250-1257.
357. Hisashi S et al.Active-site characteristics of CYP2C19 and CYP2C9 probed with hydantoin and barbiturate inhibitors. [J] Archives of Biochemistry and Biophysics.2004,429:1-15.
358. Dimitra Nikou et al. An adult-specific CYP6 P450 gene is overexpressed in a pyrethroid-resistant strain of the malaria vector, Anopheles gambiae.[J]Gene.2003,318:91-102.
359. James C Fogleman et al.Analysis of fragment homology among DNA sequences from cytochrome P450 families 4 and 6.[J]Genetica.2001,110:257-265.
360. Yu SJ et al.Biochemical characteristics of insecticide resistance in the fall armyworm, Spodoptera frugiperda (J.E. Smith).[J] Pesticide Biochemistry and Physiology.2003,77:1-11.
361. Maibeche-Coisne M et al.cDNA cloning of biotransformation enzymes belonging to the cytochrome P450 family in the antennae of the noctuid moth Mamestra brassicae.[J]Insect Molecular Biology.2002,11(3):273-281.
362. Li W et al. CYP6B cytochrome P450 monooxygenases from Papilio canadensis and Papilio glaucus—potential contributions of sequence divergence to host plant associations.[J]Insect Molecular Biology.2002,11(6):543-551.
363. Sirkku T Saarikoski et al.Identification of genetic polymorphisms of CYP2S1 in a Finnish Caucasian population.[J]Mutation Research.2004,554:267-277.
364. Alexander Archakov et al.Inventory of the cytochrome P450 superfamily.[J]J Mol Model.2001, 7:140-142.
365. Masataka Sasabe et al.Molecular analysis of CYP321A1, a novel cytochrome P450 involved in metabolism of plant allelochemicals (furanocoumarins) and insecticides (cypermethrin) in Helicoverpa zea. [J]Gene.2004,338:163-175.
366. Daisuke Endo et al.Molecular cloning of P450 aromatase from the leopard gecko and its expression in the ovary. [J] Journal of Steroid Biochemistry & Molecular Biology.2005,96:131-140.
367. Julia W Pridgeon et al.Overexpression of CYP4G19 associated with a pyrethroid-resistant strain of the German cockroach, Blattella germanica (L.).[J]Gene.2003,314:157-163.
368. Martine MaRbe'che-Coisne et al.P450 and P450 reductase cDNAs from the moth Mamestra brassicae-cloning and expression patterns in male antennae.[J]Gene.2005,346:195-203.
369. April N Tucker et al. Polymorphisms in cytochrome P4503A5 (CYP3A5).[J]Cancer Letters.2005,217:61-72.
370. Tomita T et al.Molecular mechanisms involved in increased expression of a cytochrome P450 responsible for pyrethroid resistance in the housefly,Musca domestica.[J]Insect Molecular Biology.1995,4(3):135-140.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |