介孔硅干凝胶组织修复生物材料的研究
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摘要
本研究采用溶胶-凝胶法,以表面活性剂十六烷基三甲基溴化铵(CTAB)为致孔剂,合成了介孔硅干凝胶生物医用组织修复材料。研究了原料不同配比和烧结条件对介孔硅干凝胶材料性能的影响。结果表明：正硅酸乙酯(TEOS)及水的量分别固定为300mL和400mL时,当十六烷基三甲基溴化铵加入量为40g时,合成的介孔硅干凝胶(C40)具有良好的介孔结构和外观形貌,且介孔硅干凝胶是含有大量羟基基团和Si-O键的非晶材料。
以表面活性剂三嵌段共聚物P123为模板剂,制备了含钙有序介孔硅干凝胶材料,该材料具有高的比表面积和均一的孔径。含钙介孔硅干凝胶在模拟体液里,其表面能够形成磷灰石,表明该材料具有很好的生物活性。用MC3T3-E1细胞与介孔硅干凝胶材料的联合培养,结果显示该材料能够促进细胞增殖,表明该材料具有良好的细胞相容性。含钙介孔干凝胶可以有效地促进血液凝结,随着材料用量和含钙量的增加,凝血效果增强,表明该材料具有很好的止血性能。
制备了含铜及银的介孔硅干凝胶材料,这两种材料都具有良好的介孔结构,且铜元素的引入对材料的介孔结构无明显影响。含铜介孔材料可缓释出铜离子,且对大肠杆菌和金黄色葡萄球菌有很好的抗菌性,而含银抗菌材料对大肠杆菌也具有很明显的抗菌性。用含铜介孔材料治疗新西兰大白兔皮肤创伤,结果表明：含铜抗菌材料能够促进皮肤快速愈合。两种材料的抗菌效果对比说明,银元素具有比铜元素更强的抗菌能力。通过细胞实验,研究含银介孔硅干凝胶材料的细胞相容性,结果显示：当银含量降低到一定程度时,该材料无细胞毒性。
合成了大孔径(大于15nm)结构的介孔材料PSX,其对牛血清蛋白具有强有效的吸附能力,且有很大的吸附量。用PSX与MC3T3-E1细胞联合培养,结果表明：材料对细胞没有毒性；不影响细胞的生长和粘附。因此,大孔径介孔材料具有优良的吸附和生物相容性。
In this study, mesoporous silica xerogels applied for tissue repair with disparities of ingredient ratio and heating rate were synthesized by a sol-gel method using surfactant CTAB as the pore-forming agent. It was demonstrated that C40 (prepared with 40 milligram CTAB when the amounts of TEOS and H2O were 300mL and 400mL, respectively) with excellent microscopical structure and surface morphology was non-crystal containing large amount of hydroxyl groups and Si-O bonds.
Well ordered calcium-doped mesoporous silica xerogels (m-SXC) was synthesized using surfactant triblock copolymer (P123) as the template agent. Characterization results illustrated that m-SXC with high specific surface area and homogeneous pore size was bioactive. MC3T3-E1 cells culture with materials verified that m-SXC had great biocompatibility. In addition, m-SXC possessed the function to enhance blood clotting sharply promoted with the increase of Ca amounts in m-SXC and materials amount, respectively, which indicated the remarkable hemostatic activity.
Copper and siliver-doped mesoporous silica xerogels with ideal microscopical structure were prepared, respectively. Furthermore, no apparent effect of copper element on materials' structure and composition was observed and copper ions could be released from copper-doped mesoporous silica xerogels. Mesoporous silica xerogels containing copper had significant antibacterial properties against E. coli and S. aureus, as well as those containing siliver against E. coli. Besides, we can come to the conclusion that, compared with copper ion, siliver ion performed more effectively antibacterial ability against E. coli. The goal that mesoporous silica xerogels containing siliver had no negative effect on cell viability could be achieved by reducing the siliver content in materials. In vivo wound healing experiment with New Zealand rabbits demonstrated that mesoporous silica xerogels containing copper element could promote the wound healing process.
Mesoporous silica xerogels with satisfactory BSA adsorption ability and large diameter (more than 15nm) were synthesized. It is verified that these materials had great biocompatibility without any negative impact on cell attachment and proliferation by MC3T3-E1 cells culture experiment.
以表面活性剂三嵌段共聚物P123为模板剂,制备了含钙有序介孔硅干凝胶材料,该材料具有高的比表面积和均一的孔径。含钙介孔硅干凝胶在模拟体液里,其表面能够形成磷灰石,表明该材料具有很好的生物活性。用MC3T3-E1细胞与介孔硅干凝胶材料的联合培养,结果显示该材料能够促进细胞增殖,表明该材料具有良好的细胞相容性。含钙介孔干凝胶可以有效地促进血液凝结,随着材料用量和含钙量的增加,凝血效果增强,表明该材料具有很好的止血性能。
制备了含铜及银的介孔硅干凝胶材料,这两种材料都具有良好的介孔结构,且铜元素的引入对材料的介孔结构无明显影响。含铜介孔材料可缓释出铜离子,且对大肠杆菌和金黄色葡萄球菌有很好的抗菌性,而含银抗菌材料对大肠杆菌也具有很明显的抗菌性。用含铜介孔材料治疗新西兰大白兔皮肤创伤,结果表明：含铜抗菌材料能够促进皮肤快速愈合。两种材料的抗菌效果对比说明,银元素具有比铜元素更强的抗菌能力。通过细胞实验,研究含银介孔硅干凝胶材料的细胞相容性,结果显示：当银含量降低到一定程度时,该材料无细胞毒性。
合成了大孔径(大于15nm)结构的介孔材料PSX,其对牛血清蛋白具有强有效的吸附能力,且有很大的吸附量。用PSX与MC3T3-E1细胞联合培养,结果表明：材料对细胞没有毒性；不影响细胞的生长和粘附。因此,大孔径介孔材料具有优良的吸附和生物相容性。
In this study, mesoporous silica xerogels applied for tissue repair with disparities of ingredient ratio and heating rate were synthesized by a sol-gel method using surfactant CTAB as the pore-forming agent. It was demonstrated that C40 (prepared with 40 milligram CTAB when the amounts of TEOS and H2O were 300mL and 400mL, respectively) with excellent microscopical structure and surface morphology was non-crystal containing large amount of hydroxyl groups and Si-O bonds.
Well ordered calcium-doped mesoporous silica xerogels (m-SXC) was synthesized using surfactant triblock copolymer (P123) as the template agent. Characterization results illustrated that m-SXC with high specific surface area and homogeneous pore size was bioactive. MC3T3-E1 cells culture with materials verified that m-SXC had great biocompatibility. In addition, m-SXC possessed the function to enhance blood clotting sharply promoted with the increase of Ca amounts in m-SXC and materials amount, respectively, which indicated the remarkable hemostatic activity.
Copper and siliver-doped mesoporous silica xerogels with ideal microscopical structure were prepared, respectively. Furthermore, no apparent effect of copper element on materials' structure and composition was observed and copper ions could be released from copper-doped mesoporous silica xerogels. Mesoporous silica xerogels containing copper had significant antibacterial properties against E. coli and S. aureus, as well as those containing siliver against E. coli. Besides, we can come to the conclusion that, compared with copper ion, siliver ion performed more effectively antibacterial ability against E. coli. The goal that mesoporous silica xerogels containing siliver had no negative effect on cell viability could be achieved by reducing the siliver content in materials. In vivo wound healing experiment with New Zealand rabbits demonstrated that mesoporous silica xerogels containing copper element could promote the wound healing process.
Mesoporous silica xerogels with satisfactory BSA adsorption ability and large diameter (more than 15nm) were synthesized. It is verified that these materials had great biocompatibility without any negative impact on cell attachment and proliferation by MC3T3-E1 cells culture experiment.
引文
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