生物和非生物胁迫条件下拟南芥AtCBP60g表达特性研究
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摘要
钙离子作为第二信使,参与植物生长、发育、抗逆等多种生理反应。钙调素作为一类钙离子中继传感器,主要通过与CaM结合蛋白(CaMBP)结合来调节细胞内的一些生理生化反应,在Ca~(2+)信号系统中发挥至关重要的作用。拟南芥有至少200种CaMBPs,其中CBP60s是一个由7个成员组成的家族。2002年Reddy等人从激发子处理的拟南芥cDNA文库中筛选到5个成员,但是另外两个成员:At4g31000和At5g26920(即AtCBP60g)至今功能未知,尚未在其它植物中发现其同源基因。本研究以拟南芥野生型、AtCBP60g基因敲除突变体(cbp60g)以及AtCBP60g过表达纯合体为实验材料,探讨了拟南芥AtCBP60g基因对病原微生物和非生物胁迫的响应。结果表明:
1、Atcbp60g中T-DNA插入位点在基因的第二个内含子上,Northern杂交表明cbp60g为完全敲除突变纯合体。
2、AtCBP60g表达受丁香假单胞菌(毒性和无毒)、植物激素(SA、ABA、JA)以及氧化胁迫诱导。
3、野生型、cbp60g以及过表达株系OE26-1、OE17-10接种无毒或毒性丁香假单胞菌后,PR基因的诱导表达情况均存在以下关系:突变体中的诱导表达量﹤野生型中的诱导表达量﹤过表达株系中的诱导表达量。对于受avr Rpt2诱导的基因AIG1来说,三者之间区别不明显。
4、过表达株系OE26-1、OE17-10在苗期对ABA以及高温胁迫更敏感。钙调素作为钙受体在,信号系统中起着重要的作用近年来,随着对钙调素生理根据以上实验结果,推测拟南芥AtCBP60g参与了病原菌和非生物胁迫介导的信号转导途径。
Calcium is known as a second messenger involved in plant growth, development, stress resistance and other physiological responses. And calmodulin as a calcium channel relay sensor, mainly by binding to CaM-binding protein (CaMBP) to regulate the cell physiological and biochemical reactions, plays a crucial role in Ca~(2+) signaling system. In Arabidopsis, at least 200 CaMBPs are ecoded in the genome. Five members in a small family, CBP60s, were studied by Reddy et al in 2002. But the other two members, At4g31000 and At5g26920 (ie AtCBP60g) has unknown function so far. In this study, Arabidopsis wild-type Columbia, knockout mutant of AtCBP60g gene (cbp60g) and homozygote of AtCBP60g overexpression lines were used as materials to check the reponses AtCBP60g on biotic and abiotic stress. The results showed:
1、The T-DNA insertion site located at the second intron, and the mutant was a tanscript null knockout by northern blot analysis.
2、AtCBP60g were induced by Pseudomonas syringae(virulent and avirulent), plant hormones(SA、ABA、JA),and oxidative stress.
3、In wild type, cbp60g and overexpression lines OE26-1, OE17-10, the PR gene expression levels after virulent and avirulent P. syringae infection are: cbp60g 4、At seedling stage, the AtCBP60g overexpression lines became more sensitive to heat stress and ABA compared with wild type.
The above results suggest that Arabidopsis AtCBP60g was involved in biotic and abiotic stress-mediated signal transduction pathways.
1、Atcbp60g中T-DNA插入位点在基因的第二个内含子上,Northern杂交表明cbp60g为完全敲除突变纯合体。
2、AtCBP60g表达受丁香假单胞菌(毒性和无毒)、植物激素(SA、ABA、JA)以及氧化胁迫诱导。
3、野生型、cbp60g以及过表达株系OE26-1、OE17-10接种无毒或毒性丁香假单胞菌后,PR基因的诱导表达情况均存在以下关系:突变体中的诱导表达量﹤野生型中的诱导表达量﹤过表达株系中的诱导表达量。对于受avr Rpt2诱导的基因AIG1来说,三者之间区别不明显。
4、过表达株系OE26-1、OE17-10在苗期对ABA以及高温胁迫更敏感。钙调素作为钙受体在,信号系统中起着重要的作用近年来,随着对钙调素生理根据以上实验结果,推测拟南芥AtCBP60g参与了病原菌和非生物胁迫介导的信号转导途径。
Calcium is known as a second messenger involved in plant growth, development, stress resistance and other physiological responses. And calmodulin as a calcium channel relay sensor, mainly by binding to CaM-binding protein (CaMBP) to regulate the cell physiological and biochemical reactions, plays a crucial role in Ca~(2+) signaling system. In Arabidopsis, at least 200 CaMBPs are ecoded in the genome. Five members in a small family, CBP60s, were studied by Reddy et al in 2002. But the other two members, At4g31000 and At5g26920 (ie AtCBP60g) has unknown function so far. In this study, Arabidopsis wild-type Columbia, knockout mutant of AtCBP60g gene (cbp60g) and homozygote of AtCBP60g overexpression lines were used as materials to check the reponses AtCBP60g on biotic and abiotic stress. The results showed:
1、The T-DNA insertion site located at the second intron, and the mutant was a tanscript null knockout by northern blot analysis.
2、AtCBP60g were induced by Pseudomonas syringae(virulent and avirulent), plant hormones(SA、ABA、JA),and oxidative stress.
3、In wild type, cbp60g and overexpression lines OE26-1, OE17-10, the PR gene expression levels after virulent and avirulent P. syringae infection are: cbp60g
The above results suggest that Arabidopsis AtCBP60g was involved in biotic and abiotic stress-mediated signal transduction pathways.
引文
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2 Harper J F , Breton G, Harmon A. Decoding Ca2 + signals through plant protein kinases.Annu Rev Plant Biol[J] , 2004 , 55 :263 -288
3 Reddy VS , Reddy AS. Proteomics of calcium-signaling components in plants. Phytochemistry[J] , 2004 , 65 : 1745 -1776
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5 Yang T , Poovaiah BW. Calcium/ calmodulin-mediated signal network in plants. Trends Plant Sci[J] , 2003 , 8 : 505 -512
6刘曼,毛国红,孙大业.植物钙调素亚型.植物生理学通讯[J].2005 41(1):1-5
7唐军,郭毅,孙大业.植物钙调素结合蛋白(CaMBPs)的研究概况.细胞生物学杂志, 1995, 37(3):129
8孙大业.植物细胞信号转导研究进展.植物生理学通讯.1996 (32)2: 81-91
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