天然药物没食子预防龋病的动物实验研究
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摘要
目的:通过动物实验观察和探讨天然药物没食子预防龋病的有效性和安全性,为天然药物没食子预防龋病的临床研究提供理论基础和实验依据。方法:SPF-SD大鼠感染致龋细菌,饲以致龋饲料2000#,分别用蒸馏水、没食子浸剂、氟化钠溶液、洗必泰溶液以及没食子+氟化钠混合液连续5周给5组大鼠口腔涂药,采集大鼠唾液培养,记录变形链球菌水平及龋齿Keyes评分,观察没食子及没食子+氟化钠对大鼠口腔内变形链球菌生长繁殖的影响并评估药物抑制龋损形成的效果。结果:①变形链球菌水平:与蒸馏水组相比,其余各组菌量均有不同程度的降低,只有氟化钠组和洗必泰组与蒸馏水组比较有统计学差异(P<0.05);②龋齿Keyes记分:各实验组药物对大鼠龋齿的形成及严重程度均有不同程度的抑制,其中没食子显示对窝沟面龋和光滑面龋有较好的防治效果(P<0.05),但均低于氟化钠组;没食子+氟化钠组未见明显的协同抑龋作用。③安全性评价:通过整个实验过程中大鼠的进食情况和体重、口腔黏膜安全评价,各组大鼠间未见明显差异(P>0.05)且药物口腔处理期间,大鼠的一般状况良好,口腔黏膜未见异常表现。结论:天然药物没食子能抑制大鼠口腔变链菌的生长代谢,可有效预防龋齿的发生,经口腔局部应用,未见明显毒性反应。
Objective: Based on the experimental observation of animal natural medicines Turkish gall effect of dental caries prevention, natural medicine for the prevention of dental caries Turkish gall provided the experimental basis for clinical research. Methods: SPF-SD rats infected with Streptococcus mutans, which feed feed caries 2000 #, respectively, with distilled water, infusion Turkish gall, NaF and CHX solution, as well as the Turkish gall + NaF mixture, for 5 weeks to 5 oral rats drug-coated, training records collected rat saliva levels of Streptococcus mutans and dental caries score was observed Keyes Turkish gall mouth of the rat growth and reproduction of Streptococcus mutans and evaluation of the effects of drugs inhibiting the formation of caries loss results. Results: 1. Streptococcus mutans level: Compared with the distilled water, and the rest of the group to different extents in both strains decreased, only NaF and CHX group compared with the distilled water there is significant difference (P<0. 05) ; 2. caries Keyes points: the experimental group of drugs on the formation of dental caries in rats and the severity of the suppression in varying degrees, of which Turkish gall show on sulcal-surface and smooth-surface caries had a good effect (P<0.05) , but were lower than the NaF group; Turkish Gall + NaF group had no obvious role in the synergistic inhibition of caries. 3. safety evaluation: throug the food and water consumption and the weight gains, the healty oral moucous membrane of the rats, the different groups didn’t significant difference(P>0.05),the general appearance of the rats had better, the oral moucous membrane had health during the experiment period. Conclusion: The natural medicine Turkish gall inhibited rat oral Streptococcus mutans growth and metabolism, effectively prevented the occurrence of dental caries,by that oral cavity part applies , not having seen obvious toxicity reaction.
Objective: Based on the experimental observation of animal natural medicines Turkish gall effect of dental caries prevention, natural medicine for the prevention of dental caries Turkish gall provided the experimental basis for clinical research. Methods: SPF-SD rats infected with Streptococcus mutans, which feed feed caries 2000 #, respectively, with distilled water, infusion Turkish gall, NaF and CHX solution, as well as the Turkish gall + NaF mixture, for 5 weeks to 5 oral rats drug-coated, training records collected rat saliva levels of Streptococcus mutans and dental caries score was observed Keyes Turkish gall mouth of the rat growth and reproduction of Streptococcus mutans and evaluation of the effects of drugs inhibiting the formation of caries loss results. Results: 1. Streptococcus mutans level: Compared with the distilled water, and the rest of the group to different extents in both strains decreased, only NaF and CHX group compared with the distilled water there is significant difference (P<0. 05) ; 2. caries Keyes points: the experimental group of drugs on the formation of dental caries in rats and the severity of the suppression in varying degrees, of which Turkish gall show on sulcal-surface and smooth-surface caries had a good effect (P<0.05) , but were lower than the NaF group; Turkish Gall + NaF group had no obvious role in the synergistic inhibition of caries. 3. safety evaluation: throug the food and water consumption and the weight gains, the healty oral moucous membrane of the rats, the different groups didn’t significant difference(P>0.05),the general appearance of the rats had better, the oral moucous membrane had health during the experiment period. Conclusion: The natural medicine Turkish gall inhibited rat oral Streptococcus mutans growth and metabolism, effectively prevented the occurrence of dental caries,by that oral cavity part applies , not having seen obvious toxicity reaction.
引文
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