重症肌无力患者骨髓单个核细胞中细胞周期蛋白D表达的初步观察
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摘要
目的:观察重症肌无力(Myasthenia gravis,MG)患者骨髓单个核细胞中细胞周期蛋白D(Cyclin D)不同亚型的表达。
方法:重症肌无力组10例及健康对照组5例,采集骨髓标本,用淋巴细胞分离液(Ficoll-paque)分离骨髓单个核细胞,按Trizol-氯仿-异丙醇一步法提取细胞总RNA,用反转录PCR(Reverse polymerase chain reaction,RT-PCR)方法检测Cyclin D不同亚型(D1、D2、D3)的表达。
结果:在重症肌无力组和健康对照组中,Cyclin D1都没有表达;Cyclin D2、Cyclin D3的表达,在重症肌无力组和健康对照组之间无明显差别(p﹥0.05)。
结论:本研究未发现重症肌无力患者骨髓单个核细胞Cyclin D的表达异常。
Objective: To observe the expression of Cyclin D in the bone marrow mononuclear cells of Myasthenia Gravis(MG) patients.
Methods: The bone marrow samples were collected from ten MG patients and five normal controls.The bone marrow mononuclear cells were isolated with Ficoll-paque. The total RNA was extracted according to the method of Trizol-chloroform-isopropyl alcohol.The mRNA expres- sion levels of Cyclin D isoforms were detected by semi-quantitative RT-PCR.
Results: The Cyclin D1 mRNA could not be detected in the bone marrow mononuclear cells of MG patients and normal persons, but the Cyclin D2 and Cyclin D3 could be detected.And the expression levels of Cyclin D2 and Cyclin D3 in MG patients and normal controls show no significance. (p﹥0.05)
Conclusions: The expression levels of Cyclin D in MG patients are as the same as those in normal controls shows that there is no significant cell phase transition disorders at the G1 phase in our study.
方法:重症肌无力组10例及健康对照组5例,采集骨髓标本,用淋巴细胞分离液(Ficoll-paque)分离骨髓单个核细胞,按Trizol-氯仿-异丙醇一步法提取细胞总RNA,用反转录PCR(Reverse polymerase chain reaction,RT-PCR)方法检测Cyclin D不同亚型(D1、D2、D3)的表达。
结果:在重症肌无力组和健康对照组中,Cyclin D1都没有表达;Cyclin D2、Cyclin D3的表达,在重症肌无力组和健康对照组之间无明显差别(p﹥0.05)。
结论:本研究未发现重症肌无力患者骨髓单个核细胞Cyclin D的表达异常。
Objective: To observe the expression of Cyclin D in the bone marrow mononuclear cells of Myasthenia Gravis(MG) patients.
Methods: The bone marrow samples were collected from ten MG patients and five normal controls.The bone marrow mononuclear cells were isolated with Ficoll-paque. The total RNA was extracted according to the method of Trizol-chloroform-isopropyl alcohol.The mRNA expres- sion levels of Cyclin D isoforms were detected by semi-quantitative RT-PCR.
Results: The Cyclin D1 mRNA could not be detected in the bone marrow mononuclear cells of MG patients and normal persons, but the Cyclin D2 and Cyclin D3 could be detected.And the expression levels of Cyclin D2 and Cyclin D3 in MG patients and normal controls show no significance. (p﹥0.05)
Conclusions: The expression levels of Cyclin D in MG patients are as the same as those in normal controls shows that there is no significant cell phase transition disorders at the G1 phase in our study.
引文
1 Motokura T,Bloom T,Kim HG,et al. A novel Cyclin encoded by a bcl1-linked candidate oncogene[J]. Nature.1991,350 (6318):512-515.
2 Patrick J, Lindstrom J.Autoimmune response to acetylcholine receptor. Science. 1973,180:871–872.
3 Horton RM, Manfredi AA, Conti-Tronconi B. The‘embryonic’gamma subunit of the nicotinic acetylcholine receptor is expressed in adult extraocular muscle. Neurology.1993,43:983–986.
4 Hoch W, et al. Auto-antibodies to the receptor tyrosine kinase MuSK in patients with myasthenia gravis without acetylcholine receptor antibodies. Nat Med. 2001, 7:365–368.
5 Ruegg MA, Bixby JL. Agrin orchestrates synaptic differentiation at the vertebrate neuromuscular junction. Trends Neurosci. 1998, 21:22- 27.
6 Glass DJ, et al. The receptor tyrosine kinase MuSK is required for neuromuscular junction formation and is a functional receptor for agrin. Cold Spring Harb. Symp Quant Biol. 1996, 61:435–444.
7 Medof ME, Walter EI, Rutgers JL, Knowles DM, Nussenzweig V. Identification of the complement decay-accelerating factor (DAF) on epithelium and glandular cells and in body fluids[J]. ExpMed.1987,165:848–864.
8 McNearney T, Ballard L, Seya T, Atkinson JP. Membrane cofactor protein of complement is present on human fibroblast, epithelial, and endothelial cells [J]. Clin Invest. 1989,84:538–545.
9 Meri S, Waldmann H, Lachmann PJ. Distribution of protectin (CD59), a complement membrane attack inhibitor; in normal human tissues. Lab Invest.1991, 65:532–537.
10 Lin F, et al. Markedly enhanced susceptibility to experimental autoimmune myasthenia gravis in the absence of decay-accelerating factor protection[J]. Clin Invest.2002,110:1269–1274
11 Tronconi B, Tzartos S, Lindstrom J. Monoclonal antibodies as probes of acetylcholine receptor structure .2. Binding to native receptor. Biochemistry.1981, 20:2181–2191.
12 Harris H.The Birth of the Cel1.New Haven, Yale University Press.1999.
13 Howard A,Pelc S. Synthesis of deoxyribonucleic acid in normal and irradiatedcellsand its relation to chromosome breakage.He- -redity.1953.6:261—273.
14 Xiong Y, Menninger J, Beach D, Ward DC. Molecular cloning and chromosomal mapping of CCND genes encoding human D-type cyclins. Genomics. 1992 Jul;13(3):575–584.
15 Xiong Y, Zhang H, Beach D. D type cyclins associate with multiple protein kinases and the DNA replication and repair factor PCNA.Cell.1992 Oct 30;71(3):505-14.
16 Fry DW, Bedford DC, Harvey PH, et al. Cell cycle and biochemical effects of PD0183812: A potent inhibitor of the Cyclin D-dependent kinasesCdk4 and Cdk6[J].BiolChem. 2001, 276(20): 16617-16623.
17 Scott Ely, Maurizio Di Liberto, Ruben Niesvizky, et al. Mutually Exclusive Cyclin Dependent Kinase 4/Cyclin D1 and Cyclin Depend- ent Kinase 6/Cyclin D2 Pairing Inactivates Retinoblastoma Protein and Promotes Cell Cycle Dysregulation in Multiple Myeloma. Cancer Res. Dec 2005, 65: 11345 - 11353.
18 Zi CHEN,Qiuling Wu,et al. Effects of Betulinic Acid on Proliferation and Apoptosis in Jurkat Cells and Its In Vitro Mechanism[J]. Huazhong Univ Sci Technol[Med Sci].2008, 28(6):634-638.
19 Maddison LA, HussWJ, BarriosRM, et al. Differential expression of cell cycle regulatorymolecules and evidence for a“Cyclin switch”during progression of prostate cancer[J].Prostate.2004, 58(4):335-334
20宋铁山,王欣,李旭光。异丙酚对大鼠脑缺血再灌注海马细胞周期因子变化及神经元凋亡的影响。中国病理生理杂志。2009,25(4):810—812。
21 Ekaterini Angelis, Alejandro Garcia, et al. A Cyclin D2–Rb Pathway Regulates Cardiac Myocyte Size and RNA Polymerase III AfterBiomechanical Stress in Adult Myocardium. Circ Res. May 2008; 102: 1222 - 1229.
22 Ciemerych MA, Kenney AM, Sicinska E, et a.l Development of mice expressing a single D-type Cyclin[J].Genes.2002, 16(24):3277- 3289.
23 KozarK, CiemerychMA, RebelVI, et a.l Mouse development and cell proliferation in the absence of D-Cyclins[J].Cell. 2004, 118(4): 477-491.
24 Carthon BC, Neumann CA, DasM, et a.l Genetic replacement of Cyclin D1 function inmouse developmentby CyclinD2[J].Mol Cell Biol. 2005, 5625(3): 1081-1088.
25 Young NS.Hematopoieifc cell destruction by immume mechanisms in acquired aplastic anemia[J].Semin Hematol. 2000,37(1):3--14
26 Risitano AM,Kook H, Zeng W,et a1.Oligaclonal and polyclonal CD4 and CD8 lymphocytes in aplastic anemia and paroxysmal nocturnal hemoglobinuria measured by V beta CDR3 spectmtyping and flow cytometry[J].Blood. 2002,100(1):178—183.
27 Dufour C,Corcione A,Svahn J,et al,Interferon gamma and tumour necrosis factor alpha are overexpressed in bone marrow T lymphocytes from paediatric patients with aplasitc anaemia[J].Br J Haematol. 2001,115(4):1023--1031.
28 Maciejewski JP,Selleri C,Sato T,et a1.Increased expression of Fasantigen on bone marrow CD34+ cells of patients with aplastic anaemla[J].Br J Haematol. 1995,91(1):245—252
29孟凡凯,谭细友,孙汉英等。再生障碍性贫血患者血清对脐血CD34+细胞Cyclin D关键亚型表达的影响。中国病理生理杂志。2006,22 (7):1362--l365。
30 Furukawa Y. Cell cycle regulation of hematopoietic stem cells. Hum Cell. 1998, 11: 81
31 Sherr C J. Mammalian G1 Cyclins. Cell. 1993, 73:1059
32 Della Ragione F, Borriello A, Mastropietro Set al. Expression of G1-phase cell cycle genes during hematopoieticlineage. Biochem Biophys Res Commun. 1997, 231: 73
33 Ando K, Ajchenbaum-Cymbalista F, Griffin J D. Regulation of G1/S transition by Cyclins D2 and D3 in hematopoietic cells. Proc Natl Acad Sci USA. 1993 ,90: 9571
1 BakerGL, LandisMW, Hinds PW, et al. Multiple functions of D-type Cyclins can antagonize pRb-mediated suppression of proliferation[J].CellCycle,2005, 4(2): 330-338.
2 ChytilA, Waltner-LawM, WestR, et al. Construction of a Cyclin D1-Cdk2 fusion protein to model the biological functions of Cyclin D1-Cdk2 complexes[ J].J Biol Chem,2004, 279 (46): 47688-47698.
3 Yi Zang, Li-Fang Yu, Fa-Jun Nan, Lin-Yin Feng, and Jia Li. AMP-activated Protein Kinase Is Involved in Neural Stem Cell Growth Suppression and Cell Cycle Arrest by 5-Aminoimidazole-4-carboxamide-1-β-D-ribofuranoside and Glucose Deprivation by Down-regulating Phospho-retinoblastoma Protein and Cyclin D. [J] Biol. Chem. Mar 2009,284: 6175 - 6184.
4 Tao Zhang, Wei-Dong Liu, Nicolle A. Saunee, Mary B. Breslin, and Michael S. Lan.Zinc Finger Transcription Factor INSM1 Interrupts Cyclin D1 and Cdk4 Binding and Induces Cell Cycle Arrest [J]. Biol. Chem,Feb 2009; 284: 5574 - 5581.
5 FaastR, White J, Cartwright P, et al.Cdk6-Cyclin D3 activity in murineES cells is resistant to inhibition by p16 (INK4a) [J].Oncogene,2004, 23(2): 491-502.
6 Tapan K, BaguiTK, Mohapatra S, et al.P27Kip1 and p21Cip1 are not required for the formation ofactiveD Cyclin-Cdk4 complexes[J]. MolCellBiol,2003, 23(20): 7285-7290.
7 Seo BR, Lee KW, Ha J, et al. Saucernetin-7 isolated from Saururus chinensis inhibits proliferation ofhuman promyelocyticHL-60 leukemia cellsviaG0/G1 phase arrestand induction of differentiation [J]. Carcinogenesis, 2004, 25(8): 1387-1394 .
8 Fry DW, Bedford DC, Harvey PH, et al. Cell cycle and biochemical effects ofPD 0183812: A potent inhibitor of the Cyclin D-dependent kinasesCdk4 and Cdk6. [J]BiolChem,2001, 276(20): 16617-16623.
9 Scott Ely, Maurizio Di Liberto, Ruben Niesvizky, et al. Mutually Exclusive Cyclin-Dependent Kinase 4/Cyclin D1 and Cyclin-Dependent Kinase 6/Cyclin D2 Pairing Inactivates Retinoblastoma Protein and Promotes Cell Cycle Dysregulation in Multiple Myeloma. Cancer Res., Dec 2005; 65: 11345 - 11353.
10 Zi CHEN,Qiuling wu,et al. Effects of Betulinic Acid on Proliferation and Apoptosis in Jurkat Cellsand Its / In Vitro Mechanism [ J]. Huazhong Univ Sci Technol[Med Sci],2008, 28(6):634-638.
11 Maddison LA, HussWJ, BarriosRM, et al. Differential expression of cellcycle regulatorymolecules and evidence for a“Cyclin switch”during progression of prostate cancer[ J].Prostate,2004, 58(4):335-344.
12宋铁山,王欣,李旭光。异丙酚对大鼠脑缺血再灌注海马细胞周期因子变化及神经元凋亡的影响。中国病理生理杂志,2009,25(4):810—812。
13 Ekaterini Angelis, Alejandro Garcia, et al. A Cyclin D2–Rb Pathway Regulates Cardiac Myocyte Size and RNA Polymerase III After Biomechanical Stress in Adult Myocardium. Circ. Res, May 2008; 102: 1222 - 1229.
14 Young NS.Hematopoieifc cell destruction by immume mechanisms in acquired aplastic anemia[J].Semin Hematol,2000,37(1):3--14
15 Risitano AM,Kook H, Zeng W,et a1.Oligaclonal and polyclonal CD4 and CD8 lymphocytes in aplastic anemia and paroxysmal nocturnal hemoglobinuria measured by V beta CDR3 spectmtyping and flow cytometry[J].Blood,2002,100(1):178—183.
16 Dufour C,Corcione A,Svahn J,et al,Interferon gamma and tumour necrosis factor alpha are overexpressed in bone marrow T lymphocytes from paediatric patients with aplasitc anaemia[J].Br J Haematol,2001,115(4):1023--1031.
17 Maciejewski JP,Selleri C,Sato T,et a1.Increased expression of Fas antigen on bone marrow CD34+ cells of patients with aplastic anaemla[J].Br J Haematol,1995,91(1):245—252
18孟凡凯,谭细友,孙汉英等。再生障碍性贫血患者血清对脐血CD34+细胞Cyclin D关键亚型表达的影响。中国病理生理杂志, 2006,22 (7):1362--l365。
19 Ciemerych MA, Kenney AM, Sicinska E, et a.l Development of mice expressing a single D-type Cyclin[J].Genes,2002, 16(24):3277-3289.
20 KozarK, CiemerychMA, RebelVI, et a.l Mouse development and cell proliferation in the absence ofD-Cyclins[ J].Cell,2004, 118(4): 477-491.
21 Carthon BC, Neumann CA, DasM, et a.l Genetic replacement of Cyclin D1 function inmouse developmentby CyclinD2[J].Mo.lCell Biol,2005, 5625(3): 1081-1088.
2 Patrick J, Lindstrom J.Autoimmune response to acetylcholine receptor. Science. 1973,180:871–872.
3 Horton RM, Manfredi AA, Conti-Tronconi B. The‘embryonic’gamma subunit of the nicotinic acetylcholine receptor is expressed in adult extraocular muscle. Neurology.1993,43:983–986.
4 Hoch W, et al. Auto-antibodies to the receptor tyrosine kinase MuSK in patients with myasthenia gravis without acetylcholine receptor antibodies. Nat Med. 2001, 7:365–368.
5 Ruegg MA, Bixby JL. Agrin orchestrates synaptic differentiation at the vertebrate neuromuscular junction. Trends Neurosci. 1998, 21:22- 27.
6 Glass DJ, et al. The receptor tyrosine kinase MuSK is required for neuromuscular junction formation and is a functional receptor for agrin. Cold Spring Harb. Symp Quant Biol. 1996, 61:435–444.
7 Medof ME, Walter EI, Rutgers JL, Knowles DM, Nussenzweig V. Identification of the complement decay-accelerating factor (DAF) on epithelium and glandular cells and in body fluids[J]. ExpMed.1987,165:848–864.
8 McNearney T, Ballard L, Seya T, Atkinson JP. Membrane cofactor protein of complement is present on human fibroblast, epithelial, and endothelial cells [J]. Clin Invest. 1989,84:538–545.
9 Meri S, Waldmann H, Lachmann PJ. Distribution of protectin (CD59), a complement membrane attack inhibitor; in normal human tissues. Lab Invest.1991, 65:532–537.
10 Lin F, et al. Markedly enhanced susceptibility to experimental autoimmune myasthenia gravis in the absence of decay-accelerating factor protection[J]. Clin Invest.2002,110:1269–1274
11 Tronconi B, Tzartos S, Lindstrom J. Monoclonal antibodies as probes of acetylcholine receptor structure .2. Binding to native receptor. Biochemistry.1981, 20:2181–2191.
12 Harris H.The Birth of the Cel1.New Haven, Yale University Press.1999.
13 Howard A,Pelc S. Synthesis of deoxyribonucleic acid in normal and irradiatedcellsand its relation to chromosome breakage.He- -redity.1953.6:261—273.
14 Xiong Y, Menninger J, Beach D, Ward DC. Molecular cloning and chromosomal mapping of CCND genes encoding human D-type cyclins. Genomics. 1992 Jul;13(3):575–584.
15 Xiong Y, Zhang H, Beach D. D type cyclins associate with multiple protein kinases and the DNA replication and repair factor PCNA.Cell.1992 Oct 30;71(3):505-14.
16 Fry DW, Bedford DC, Harvey PH, et al. Cell cycle and biochemical effects of PD0183812: A potent inhibitor of the Cyclin D-dependent kinasesCdk4 and Cdk6[J].BiolChem. 2001, 276(20): 16617-16623.
17 Scott Ely, Maurizio Di Liberto, Ruben Niesvizky, et al. Mutually Exclusive Cyclin Dependent Kinase 4/Cyclin D1 and Cyclin Depend- ent Kinase 6/Cyclin D2 Pairing Inactivates Retinoblastoma Protein and Promotes Cell Cycle Dysregulation in Multiple Myeloma. Cancer Res. Dec 2005, 65: 11345 - 11353.
18 Zi CHEN,Qiuling Wu,et al. Effects of Betulinic Acid on Proliferation and Apoptosis in Jurkat Cells and Its In Vitro Mechanism[J]. Huazhong Univ Sci Technol[Med Sci].2008, 28(6):634-638.
19 Maddison LA, HussWJ, BarriosRM, et al. Differential expression of cell cycle regulatorymolecules and evidence for a“Cyclin switch”during progression of prostate cancer[J].Prostate.2004, 58(4):335-334
20宋铁山,王欣,李旭光。异丙酚对大鼠脑缺血再灌注海马细胞周期因子变化及神经元凋亡的影响。中国病理生理杂志。2009,25(4):810—812。
21 Ekaterini Angelis, Alejandro Garcia, et al. A Cyclin D2–Rb Pathway Regulates Cardiac Myocyte Size and RNA Polymerase III AfterBiomechanical Stress in Adult Myocardium. Circ Res. May 2008; 102: 1222 - 1229.
22 Ciemerych MA, Kenney AM, Sicinska E, et a.l Development of mice expressing a single D-type Cyclin[J].Genes.2002, 16(24):3277- 3289.
23 KozarK, CiemerychMA, RebelVI, et a.l Mouse development and cell proliferation in the absence of D-Cyclins[J].Cell. 2004, 118(4): 477-491.
24 Carthon BC, Neumann CA, DasM, et a.l Genetic replacement of Cyclin D1 function inmouse developmentby CyclinD2[J].Mol Cell Biol. 2005, 5625(3): 1081-1088.
25 Young NS.Hematopoieifc cell destruction by immume mechanisms in acquired aplastic anemia[J].Semin Hematol. 2000,37(1):3--14
26 Risitano AM,Kook H, Zeng W,et a1.Oligaclonal and polyclonal CD4 and CD8 lymphocytes in aplastic anemia and paroxysmal nocturnal hemoglobinuria measured by V beta CDR3 spectmtyping and flow cytometry[J].Blood. 2002,100(1):178—183.
27 Dufour C,Corcione A,Svahn J,et al,Interferon gamma and tumour necrosis factor alpha are overexpressed in bone marrow T lymphocytes from paediatric patients with aplasitc anaemia[J].Br J Haematol. 2001,115(4):1023--1031.
28 Maciejewski JP,Selleri C,Sato T,et a1.Increased expression of Fasantigen on bone marrow CD34+ cells of patients with aplastic anaemla[J].Br J Haematol. 1995,91(1):245—252
29孟凡凯,谭细友,孙汉英等。再生障碍性贫血患者血清对脐血CD34+细胞Cyclin D关键亚型表达的影响。中国病理生理杂志。2006,22 (7):1362--l365。
30 Furukawa Y. Cell cycle regulation of hematopoietic stem cells. Hum Cell. 1998, 11: 81
31 Sherr C J. Mammalian G1 Cyclins. Cell. 1993, 73:1059
32 Della Ragione F, Borriello A, Mastropietro Set al. Expression of G1-phase cell cycle genes during hematopoieticlineage. Biochem Biophys Res Commun. 1997, 231: 73
33 Ando K, Ajchenbaum-Cymbalista F, Griffin J D. Regulation of G1/S transition by Cyclins D2 and D3 in hematopoietic cells. Proc Natl Acad Sci USA. 1993 ,90: 9571
1 BakerGL, LandisMW, Hinds PW, et al. Multiple functions of D-type Cyclins can antagonize pRb-mediated suppression of proliferation[J].CellCycle,2005, 4(2): 330-338.
2 ChytilA, Waltner-LawM, WestR, et al. Construction of a Cyclin D1-Cdk2 fusion protein to model the biological functions of Cyclin D1-Cdk2 complexes[ J].J Biol Chem,2004, 279 (46): 47688-47698.
3 Yi Zang, Li-Fang Yu, Fa-Jun Nan, Lin-Yin Feng, and Jia Li. AMP-activated Protein Kinase Is Involved in Neural Stem Cell Growth Suppression and Cell Cycle Arrest by 5-Aminoimidazole-4-carboxamide-1-β-D-ribofuranoside and Glucose Deprivation by Down-regulating Phospho-retinoblastoma Protein and Cyclin D. [J] Biol. Chem. Mar 2009,284: 6175 - 6184.
4 Tao Zhang, Wei-Dong Liu, Nicolle A. Saunee, Mary B. Breslin, and Michael S. Lan.Zinc Finger Transcription Factor INSM1 Interrupts Cyclin D1 and Cdk4 Binding and Induces Cell Cycle Arrest [J]. Biol. Chem,Feb 2009; 284: 5574 - 5581.
5 FaastR, White J, Cartwright P, et al.Cdk6-Cyclin D3 activity in murineES cells is resistant to inhibition by p16 (INK4a) [J].Oncogene,2004, 23(2): 491-502.
6 Tapan K, BaguiTK, Mohapatra S, et al.P27Kip1 and p21Cip1 are not required for the formation ofactiveD Cyclin-Cdk4 complexes[J]. MolCellBiol,2003, 23(20): 7285-7290.
7 Seo BR, Lee KW, Ha J, et al. Saucernetin-7 isolated from Saururus chinensis inhibits proliferation ofhuman promyelocyticHL-60 leukemia cellsviaG0/G1 phase arrestand induction of differentiation [J]. Carcinogenesis, 2004, 25(8): 1387-1394 .
8 Fry DW, Bedford DC, Harvey PH, et al. Cell cycle and biochemical effects ofPD 0183812: A potent inhibitor of the Cyclin D-dependent kinasesCdk4 and Cdk6. [J]BiolChem,2001, 276(20): 16617-16623.
9 Scott Ely, Maurizio Di Liberto, Ruben Niesvizky, et al. Mutually Exclusive Cyclin-Dependent Kinase 4/Cyclin D1 and Cyclin-Dependent Kinase 6/Cyclin D2 Pairing Inactivates Retinoblastoma Protein and Promotes Cell Cycle Dysregulation in Multiple Myeloma. Cancer Res., Dec 2005; 65: 11345 - 11353.
10 Zi CHEN,Qiuling wu,et al. Effects of Betulinic Acid on Proliferation and Apoptosis in Jurkat Cellsand Its / In Vitro Mechanism [ J]. Huazhong Univ Sci Technol[Med Sci],2008, 28(6):634-638.
11 Maddison LA, HussWJ, BarriosRM, et al. Differential expression of cellcycle regulatorymolecules and evidence for a“Cyclin switch”during progression of prostate cancer[ J].Prostate,2004, 58(4):335-344.
12宋铁山,王欣,李旭光。异丙酚对大鼠脑缺血再灌注海马细胞周期因子变化及神经元凋亡的影响。中国病理生理杂志,2009,25(4):810—812。
13 Ekaterini Angelis, Alejandro Garcia, et al. A Cyclin D2–Rb Pathway Regulates Cardiac Myocyte Size and RNA Polymerase III After Biomechanical Stress in Adult Myocardium. Circ. Res, May 2008; 102: 1222 - 1229.
14 Young NS.Hematopoieifc cell destruction by immume mechanisms in acquired aplastic anemia[J].Semin Hematol,2000,37(1):3--14
15 Risitano AM,Kook H, Zeng W,et a1.Oligaclonal and polyclonal CD4 and CD8 lymphocytes in aplastic anemia and paroxysmal nocturnal hemoglobinuria measured by V beta CDR3 spectmtyping and flow cytometry[J].Blood,2002,100(1):178—183.
16 Dufour C,Corcione A,Svahn J,et al,Interferon gamma and tumour necrosis factor alpha are overexpressed in bone marrow T lymphocytes from paediatric patients with aplasitc anaemia[J].Br J Haematol,2001,115(4):1023--1031.
17 Maciejewski JP,Selleri C,Sato T,et a1.Increased expression of Fas antigen on bone marrow CD34+ cells of patients with aplastic anaemla[J].Br J Haematol,1995,91(1):245—252
18孟凡凯,谭细友,孙汉英等。再生障碍性贫血患者血清对脐血CD34+细胞Cyclin D关键亚型表达的影响。中国病理生理杂志, 2006,22 (7):1362--l365。
19 Ciemerych MA, Kenney AM, Sicinska E, et a.l Development of mice expressing a single D-type Cyclin[J].Genes,2002, 16(24):3277-3289.
20 KozarK, CiemerychMA, RebelVI, et a.l Mouse development and cell proliferation in the absence ofD-Cyclins[ J].Cell,2004, 118(4): 477-491.
21 Carthon BC, Neumann CA, DasM, et a.l Genetic replacement of Cyclin D1 function inmouse developmentby CyclinD2[J].Mo.lCell Biol,2005, 5625(3): 1081-1088.