嗜麦芽窄食单胞菌对水体中芘、镉的修复研究
摘要
本论文利用嗜麦芽窄食单胞菌为实验菌株,对水体中芘、镉的微生物修复进行了研究,主要内容包括：嗜麦芽窄食单胞菌的生长条件优化、菌株对芘的降解性能、菌株对镉的吸附性能、水-沉积物中芘-镉复合污染的修复、微生物修复机理的初步探索。
嗜麦芽窄食单胞菌能够利用芘作为唯一碳源生长,在温度25℃、pH 8.0时生长量最大。芘浓度为0.5-2mg/L的范围内,菌体生长良好,当芘浓度大于2mg/L时,芘对菌体产生毒害作用,抑制菌体的生长。在芘浓度为2mg/L,镉浓度分别为0.1-10mg/L的复合污染体系中,菌的生长受到明显抑制,菌体的生长量随着镉浓度的增大不断降低。
在芘浓度为2mg/L时,菌体降解4d后,芘最高降解率达到65.74%。当温度为25℃和pH为8.0时,芘降解效果最好。复合污染体系中0.1-0.5mg/L镉的存在促进了菌体对芘的降解作用,而当镉浓度大于0.5mg/L后,芘的降解受到抑制。
嗜麦芽窄食单胞菌吸附镉的最适宜菌浓度为2g/L,温度在25℃时其吸附率最高,在pH 5.0达到最大吸附率93.73%。菌体对镉的吸附较好地符合Langmuir吸附等温式。FTIR分析发现,C-H、N-H、O-H作为主要官能团参与了嗜麦芽窄食单胞菌对镉的吸附过程。
嗜麦芽窄食单胞菌能有效地修复水-沉积物中芘-镉复合污染,在处理9d后,菌对芘的降解率为75.06%,1mg/L镉的残留浓度为0.175mg/L
SEM观察表明,各污染体系中菌体基本能保持正常的生长状态,从而保证菌体能有效地发挥降解和吸附作用。在菌体降解芘和吸附镉的过程中,伴随着阳离子Na+、NH4+、10、Mg2+和Ca2+的释放。FTIR分析表明,复合污染体系中,菌体降解芘和吸附镉的过程主要与羟基、酰胺基团、C-H键有关。
The biodegradation of pyrene and biosorption of cadmium by a bacterial strain (Stenotrophomonas maltophilia) were studied in this research, including the characteristics of S.maltophilia growth, biodegradation and biosorption capability, the treatment of pyrene-Cd combined pollution in water-sediment systems with strain, and the interaction mechanism between strain and pyrene-Cd.
S.maltophilia could utilize pyrene as the sole carbon source optimally at 25℃and pH 8.0. The strain grew well when pyrene concentration was 0.5 to 2mg/L. However, excess pyrene would inhibit the cultivation. The growth of bacteria was significantly affected in combined pollution system when pyrene concentration was 2mg/L and Cd concentration was 0.1 to 10mg/L, cell growth decreased with increasing cadmium concentration.
The highest degradation efficiency was 65.74% when initial pyrene concentration, temperature and pH value were 2mg/L,25℃and 8.0, respectively. The degradation of pyrene was promoted in complex pollution system with cadmium concentration 0.1 to 0.5mg/L. Nevertheless, higher concentration cadmium would depress the degradation.
The biosorption equilibrium conformed to the Langmuir equations. Infrared spectroscopy analysis indicated that the main functional groups participated in Cd adsorption were C-H, N-H, O-H.
S.maltophilia treated pyrene-Cd combined pollution in water-sediment systems effectively. The degradation efficiency of pyrene was 75.06% and the concentration of cadmium in water was decreased to 0.175mg/L from initial lmg/L after nine days.
Scanning electronic microscope (SEM) observation exhibited that S.maltophilia could maintain normal growth condition in pollution system, thus ensuring their basic capability of degradation and biosorption. The strain would release Na+, NH4+, K+, Mg2+ and Ca2+ during the process of degradation and biosorption. FTIR analysis showed that the remediation process mainly related with hydroxyl, amide groups, C-H.
嗜麦芽窄食单胞菌能够利用芘作为唯一碳源生长,在温度25℃、pH 8.0时生长量最大。芘浓度为0.5-2mg/L的范围内,菌体生长良好,当芘浓度大于2mg/L时,芘对菌体产生毒害作用,抑制菌体的生长。在芘浓度为2mg/L,镉浓度分别为0.1-10mg/L的复合污染体系中,菌的生长受到明显抑制,菌体的生长量随着镉浓度的增大不断降低。
在芘浓度为2mg/L时,菌体降解4d后,芘最高降解率达到65.74%。当温度为25℃和pH为8.0时,芘降解效果最好。复合污染体系中0.1-0.5mg/L镉的存在促进了菌体对芘的降解作用,而当镉浓度大于0.5mg/L后,芘的降解受到抑制。
嗜麦芽窄食单胞菌吸附镉的最适宜菌浓度为2g/L,温度在25℃时其吸附率最高,在pH 5.0达到最大吸附率93.73%。菌体对镉的吸附较好地符合Langmuir吸附等温式。FTIR分析发现,C-H、N-H、O-H作为主要官能团参与了嗜麦芽窄食单胞菌对镉的吸附过程。
嗜麦芽窄食单胞菌能有效地修复水-沉积物中芘-镉复合污染,在处理9d后,菌对芘的降解率为75.06%,1mg/L镉的残留浓度为0.175mg/L
SEM观察表明,各污染体系中菌体基本能保持正常的生长状态,从而保证菌体能有效地发挥降解和吸附作用。在菌体降解芘和吸附镉的过程中,伴随着阳离子Na+、NH4+、10、Mg2+和Ca2+的释放。FTIR分析表明,复合污染体系中,菌体降解芘和吸附镉的过程主要与羟基、酰胺基团、C-H键有关。
The biodegradation of pyrene and biosorption of cadmium by a bacterial strain (Stenotrophomonas maltophilia) were studied in this research, including the characteristics of S.maltophilia growth, biodegradation and biosorption capability, the treatment of pyrene-Cd combined pollution in water-sediment systems with strain, and the interaction mechanism between strain and pyrene-Cd.
S.maltophilia could utilize pyrene as the sole carbon source optimally at 25℃and pH 8.0. The strain grew well when pyrene concentration was 0.5 to 2mg/L. However, excess pyrene would inhibit the cultivation. The growth of bacteria was significantly affected in combined pollution system when pyrene concentration was 2mg/L and Cd concentration was 0.1 to 10mg/L, cell growth decreased with increasing cadmium concentration.
The highest degradation efficiency was 65.74% when initial pyrene concentration, temperature and pH value were 2mg/L,25℃and 8.0, respectively. The degradation of pyrene was promoted in complex pollution system with cadmium concentration 0.1 to 0.5mg/L. Nevertheless, higher concentration cadmium would depress the degradation.
The biosorption equilibrium conformed to the Langmuir equations. Infrared spectroscopy analysis indicated that the main functional groups participated in Cd adsorption were C-H, N-H, O-H.
S.maltophilia treated pyrene-Cd combined pollution in water-sediment systems effectively. The degradation efficiency of pyrene was 75.06% and the concentration of cadmium in water was decreased to 0.175mg/L from initial lmg/L after nine days.
Scanning electronic microscope (SEM) observation exhibited that S.maltophilia could maintain normal growth condition in pollution system, thus ensuring their basic capability of degradation and biosorption. The strain would release Na+, NH4+, K+, Mg2+ and Ca2+ during the process of degradation and biosorption. FTIR analysis showed that the remediation process mainly related with hydroxyl, amide groups, C-H.
引文
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