沙棘黄酮提取工艺及抗氧化性能的研究
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摘要
本文以沙棘黄酮类化合物为研究对象,利用超声波法提取沙棘总黄酮,并对超声波法与常规法提取沙棘黄酮的得率进行比较,得到沙棘黄酮提取的最佳工艺条件。同时利用树脂、聚酰胺柱层析及高效液相色谱的分离纯化技术分离出沙棘黄酮单体,通过抗氧化实验得到抗氧化能力最强的沙棘黄酮单体。
在超声波提取沙棘总黄酮最佳工艺条件建立过程中,选择了超声波提取时间、乙醇浓度、提取温度、物料比、超声波功率等因素进行梯度实验。最佳提取条件65%的乙醇溶液作提取剂,以1:20(g/mL)的料液比于78℃的条件下提取时间40min,超声波功率为60%,在此条件下沙棘果渣提取总黄酮得率为0.4829%。其提取时间短、能耗低可降低生产成本,提高经济效益。本实验还建立了聚酰胺柱层析分离沙棘黄酮的各项工艺参数,并得到三种沙棘黄酮单体化合物,其中第三个主峰在其的浓度为100mg/L时可以清除邻苯三酚自氧化反应产生的超氧阴离子自由基,清除率为32.2%。为开发和利用该资源提供了有益的参考。
Flavanones in Sea-buckthorn was studied in this paper. Total flavones was extracted with ultrasonic wave method. It compared the productivity of flavones which were extracted with Conventional method to ultrasonic wave method, and demonstrated the best condition of extracting flavones in Sea-buckthorn. The monomer compounds were gained by the purification technology which used Resin, Polyamide column chromatography and high performance liquid chromatogramphy. The monomer compounds which have the strongest antioxidant capacity also was gained by the experiment of antioxidant.
The experiment chose the ultrasonic wave to withdraw the time, the ethyl alcohol density, withdraws factor and so on temperature, material ratio, ultrasonic wave power carries on the gradient experiment, Discovers supersonic method withdraws the sea-buckthorn marc total flavanone the optimum condition. The best withdraws condition is 65% ethyl alcohol solution does with the quality score withdraws the medicinal preparation, by 1:20 (g/mL) the material fluid compares under 78℃conditions withdraws time 40min, the ultrasonic wave power is 60%, the sea-buckthorn marc withdraws the total flavanone under this condition rate is 0.4829%.But the convention returns to the conversion of sixteenths to decimal best to withdraw under the condition in it to have to withdraw for 3 hours, the total flavanone rate reaches as high as 0.6911%.The result indicated the ultrasonic wave law withdraws in the sea-buckthorn fruit the total flavanone rate to be inferior to the convention returns to the conversion of sixteenths to decimal to withdraw in the sea-buckthorn fruit the total flavanone rate high, but the ultrasonic wave law has does not destroy the effective component the characteristic, it withdraws the time short, the energy consumption is low may reduce the production cost, enhances the economic efficiency. The adsorption chromatography column of polyamide was also carefully studied in this article, and gained three kinds of flavones. The third crest of the wave can scavenge the superoxide anions that were produced from the self-oxidation reaction of Pyrogallol and when the concentration of the third compound was 100mg/L, the eliminating ratio was 32.2%. From the experiments, We have known the best extracted technology of seabucktborn flavones, and. it was develops and uses this resources to provide the beneficial reference.
在超声波提取沙棘总黄酮最佳工艺条件建立过程中,选择了超声波提取时间、乙醇浓度、提取温度、物料比、超声波功率等因素进行梯度实验。最佳提取条件65%的乙醇溶液作提取剂,以1:20(g/mL)的料液比于78℃的条件下提取时间40min,超声波功率为60%,在此条件下沙棘果渣提取总黄酮得率为0.4829%。其提取时间短、能耗低可降低生产成本,提高经济效益。本实验还建立了聚酰胺柱层析分离沙棘黄酮的各项工艺参数,并得到三种沙棘黄酮单体化合物,其中第三个主峰在其的浓度为100mg/L时可以清除邻苯三酚自氧化反应产生的超氧阴离子自由基,清除率为32.2%。为开发和利用该资源提供了有益的参考。
Flavanones in Sea-buckthorn was studied in this paper. Total flavones was extracted with ultrasonic wave method. It compared the productivity of flavones which were extracted with Conventional method to ultrasonic wave method, and demonstrated the best condition of extracting flavones in Sea-buckthorn. The monomer compounds were gained by the purification technology which used Resin, Polyamide column chromatography and high performance liquid chromatogramphy. The monomer compounds which have the strongest antioxidant capacity also was gained by the experiment of antioxidant.
The experiment chose the ultrasonic wave to withdraw the time, the ethyl alcohol density, withdraws factor and so on temperature, material ratio, ultrasonic wave power carries on the gradient experiment, Discovers supersonic method withdraws the sea-buckthorn marc total flavanone the optimum condition. The best withdraws condition is 65% ethyl alcohol solution does with the quality score withdraws the medicinal preparation, by 1:20 (g/mL) the material fluid compares under 78℃conditions withdraws time 40min, the ultrasonic wave power is 60%, the sea-buckthorn marc withdraws the total flavanone under this condition rate is 0.4829%.But the convention returns to the conversion of sixteenths to decimal best to withdraw under the condition in it to have to withdraw for 3 hours, the total flavanone rate reaches as high as 0.6911%.The result indicated the ultrasonic wave law withdraws in the sea-buckthorn fruit the total flavanone rate to be inferior to the convention returns to the conversion of sixteenths to decimal to withdraw in the sea-buckthorn fruit the total flavanone rate high, but the ultrasonic wave law has does not destroy the effective component the characteristic, it withdraws the time short, the energy consumption is low may reduce the production cost, enhances the economic efficiency. The adsorption chromatography column of polyamide was also carefully studied in this article, and gained three kinds of flavones. The third crest of the wave can scavenge the superoxide anions that were produced from the self-oxidation reaction of Pyrogallol and when the concentration of the third compound was 100mg/L, the eliminating ratio was 32.2%. From the experiments, We have known the best extracted technology of seabucktborn flavones, and. it was develops and uses this resources to provide the beneficial reference.
引文
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[2] Kudritskaya S E, Zagorodskaya L M,Shishkina E E. Carotenoids of the Sea Buckthorn Varietyobilnaya[J]. Khim Prir Soedin.1989, (6):852~853.
[3] Novruzov E N. Carotenoids and Sterols of Some Forms of Hippophae rhamnoides[J]. Khim Prir Soedin.1981, (1):98~99.
[4] Zhmyrko T G, Gigienova E l, Vmarov A V. Vitamins From the Oils of Hippophae rhamnoides fruit[J].Khim Prir Soedin. 1978, (3): 313~317.
[5] 葛孝炎,史国富,马翠英.沙棘化学成分的研究概况[J].中草药.1986, 17(8):42~44.
[6] 张鞍灵,高锦明.中国沙棘果实化学成分初步研究[J].陕西林业科技. 1999,2:14~15.
[7] 彭芳.黄酮类化合物的生物学作用[J].大理医学院学报.1998,7(4):52.
[8] 张康健,王蓝主编.药用植物资源开发利用学[M].北京:中国林业出版社, 1999(10): 156~164.
[9] 王玉洁,沈薇.复方沙棘油对动物烧伤烫伤及创伤的试验研究[J].国际沙棘研究与开发.2004,2.
[10] Khizhai A A. The Therqapeutic and Prophyl Actic Anti- ulcer Action of Marigold (Tagetes patula L.) and Seabukcthorn (Hippophae) Oil Sneurogenic Ulcerative lesions caused by Immobilization, Noise, and Vibration. LikSprava, 1998,(1): 172~176.
[11] Degtiareva II,Toteva Ets,Litinskaia EV,et al. Degree of Lipid Peroxidation and Vitamin E Level during the Treatment of Peptic Ulcer. Klin Med (Mosk).1991, 69 (7):38~42.
[12] Tsimmerman Ias, Mikhailovskaia LV. Effect of Sea Buckthorn Oil on Various Pathophysiologic Mechanisms and the Course of Pepticulcer.Klin Med (Mosk). 1987, Feb: 65 (2):77~82.
[13] Xiao M, Yang Z, Jiu M, et al .The Antigastroulcerative Activity of Beta-sitosterol-beta-D-glucoside and Iits Aglycone in Rats.Hua His Ko Ta Hsueh Hsueh Pao.1992, 23(1):98~101.
[14] 李守汉.沙棘油对运动大鼠部分组织及红细胞抗氧化作用的试验研究[D].硕士学位论文.2001,36~40.
[15] 付桂香,赵世萍,冯瑞芝等.高效液相色谱法测定沙棘叶中黄酮含量[J].中国中药杂志.2002,15(4):234.
[16] 徐铭渔,孙小宣,童文新.沙棘的医药研究和开发[J].沙棘.1994,3(5):15~18
[17] 陈玉香,张丽萍. 沙棘果水溶性多糖 H-n 的分离纯化与抗病毒研究[J].东北师大学报.1997,4:28~32..
[18] 章平,孙斌,王露,瞿伟菁.沙棘籽黄酮类化合物诱导人乳腺癌 Bcap-37 细胞凋亡的研究[J].东北师大学报.2004,4:35~38.
[19] 刘锡建.沙棘果渣总黄酮的提取、精制和抗氧化性能的研究[D].优秀硕士论文.2004,60~65.
[20] 草群华.从沙棘初级利用废物中提取黄酮及其降血糖药理研究[D]. 优秀硕士论文.2004,16~19.
[21] 句海松等.沙棘总黄酮对活性氧自由基的消除作用[J]. 中国药理学. 1990, 6(2): 97~101.
[22] 王家良等. 醋柳总黄酮治疗缺血型心脏病的观察疗效[J]. 四川医学院学报. 1982,(1):6.
[23] 黄良月(四川省中药研究所), 刘常伍, 范维衡等. 醋柳总黄酮防治冠心病药理基础研究[J]. 华西药学杂志.1986, 1(3):163~165.
[24] 陈星, 闫洁坤, 雷耕耘等. 沙棘果加工分离物营养成份分析简报[J]. 沙棘, 1995, 8(3):34~35.
[25] 钟飞,蒋韵,吴芬芬等.沙棘总黄酮对小鼠细胞免疫功能的影响[J].中国药理学报.1989, 5(5):307.
[26] 哈斯格日乐,刘朝.沙棘总黄酮对小鼠腹腔巨噬细胞功能的作用[J].内蒙古医学院学报.1992, 14(2):88~91.
[27] 钟飞,蒋韵,吴芬芬等.沙棘总黄酮对动物非特异性免疫功能的影响[J]. 山西医药杂志.1989, 18(1):910.
[28] 句海松,李小洁,赵保路等.沙棘总黄酮对活性氧自由基的清除作用[J].中国药理学通报.1990,6(2):9.
[29] 张振贤,胡文文.醋柳黄酮片治疗冠心病心绞痛 101 例.辽宁中医杂志[J]. 1997,24(6):258.
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