35%硫丹乳油在棉叶、棉籽和土壤中的残留分析方法及残留消解动态研究
摘要
硫丹是目前国内唯一用于防治棉铃虫、棉蚜的有机氯杀虫剂,为了能够充分发挥硫丹在生产中的农药保护作用,评价硫丹在棉花上的残留情况以及对土壤环境的影响显得尤为重要。本试验是以35%硫丹乳油为研究对象进行了一年两地的残留分析试验。建立了硫丹在棉叶、棉籽和土壤中的残留分析方法,根据田间试验结果,推荐出35%硫丹乳油在棉花中防治棉铃虫、棉蚜的合理使用准则。
硫丹的残留分析方法研究:以棉叶、棉籽和土壤为研究材料,采用气相色谱法,通过实验对不同残留分析方法进行了比较。结果表明:棉叶和棉籽样品用乙腈、氟罗里硅土固相萃取小柱净化,后经气相色谱检测。在棉叶、棉籽中的添加回收率分别为82.0%～95.7%和82.9%～95.6%,变异系数分别为6.9%～13%和3.2%～20%。土壤样品用丙酮提取,石油醚液-液萃取,旋转蒸发仪浓缩后,经氟罗里硅土固相萃取小柱净化,后经气相色谱仪检测,硫丹在土壤中的添加回收率在87.6%～104%之间,变异系数在6.2%～21%之间。在实验过程中,对样品前处理和色谱分离条件进行了详细的研究和优化,最终确证以上方法简便、准确、快速、净化效果好,满足农药残留分析的要求。
消解动态试验:按照一次施药多次采样的方法对35%硫丹乳油在棉叶和土壤中的消解动态进行研究。结果表明:在石家庄市,硫丹在棉叶中的消解动态方程为C_T=33.6e~(-0.117T),在土壤中的消解动态方程为C_T=2.38e~(-0.0348T),相关系数分别为-0.9491和-0.8368,消解半衰期分别为5.92d和19.92d。在长沙市,硫丹在棉叶和土壤中的消解动态方程分别为C_T=31.3e~(-0.116T)和C_T=9.20e~(-0.0460T),相关系数分别为-0.8597和-0.9521,消解半衰期分别为5.99d和15.07d。硫丹在不同时期在棉叶和土壤中的消解动态均符合一级动力学反应模式,都具有较高的相关性。硫丹在棉叶中的消解速率较土壤中快,石家庄地区的消解速率也不同于长沙地区。
最终残留试验:在945a.i.g/hm~2、1890a.i.g/hm~2的施药水平下,施药3次、4次,每次施药间隔期为14d,施药后距采收间隔期为7d,最终残留试验结果表明:在石家庄市,硫丹在棉叶中的最终残留量为0.492-13.2mg/kg,在土壤中的最终残留量为0.196-0.851mg/kg。在长沙市,硫丹在棉叶中的最终残留量为0.421-1.58mg/kg,在土壤中的最终残留量为0.114-0.258mg/kg,在棉籽中两地均未检出有硫丹残留,从数据分析来看:硫丹的最终残留量与施药浓度、施药次数呈正相关,施药浓度越高,施药次数越多,残留量越高;与采收间隔期呈负相关,且棉叶中的残留量高于土壤中。
联合国粮农组织(FAO/WTO)制定其标准为:硫丹在棉花籽中的最高残留限量(MRL)为1.0mg/kg。我国的推荐性国家标准:硫丹在棉籽中的最高残留限量为1.0mg/kg。由硫丹在棉花中的最终残留试验结果,我们可以看出35%硫丹乳油用于棉花害虫防治的过程中,按推荐剂量945a.i.g/hm~2和加倍剂量1890a.i.g/hm~2使用,其残留量均低于限定标准,在棉籽中均没有检测出硫丹的残留。因此,根据本研究,暂建议在棉花的生长时期,35%硫丹乳油使用剂量为推荐剂量945a.i.g/hm~2和加倍剂量1890a.i.g/hm~2,施药3到4次且最大残留限量为1.0mg/kg,在棉花中的安全间隔期为14d,在此安全间隔期内施药对环境安全,收获棉花时其棉籽中未检出有硫丹残留,其残留量满足残留标准要求,是安全的。
As a high efficiency Organochlorine insecticide, endosulfan is only pesticide that use in prevention cotton bollworm and cotton aphid. In agricultural production, endosulfan will take a key role in the future. In order to use endosulfan in science way, evaluate the residue level in cotton and affect in soil. In this paper, one-year residue-assignment of endosulfan was carried out in different Places. A method was studied for the analysis of endosulfan residue in cotton, cottonseed and soil and 35% endosulfan EC had been recommended to control cotton bollworm in cotton according to the results of field experiment.
Residue analysis methods research on endosulfan, gas chromatography method has been used in cotton、cottonseed and soil, different methods have been compared by experiments. The results show, cotton and cottonseed sample were extracted with acetonitrile, after the extract was cleaned up with a Florisil column, endosulfan was analyzed by GC, the average recoveries of cotton ranged from 82.0% to 95.7%, the C.V was between 6.9% to 13%. The average recoveries of cottonseed ranged from 82.9% to 95.6%, the C.V was 3.2% to 20%. Soil sample was extracted with acetone, LLE by petroleum ether, the average recoveries of soil ranged from 87.6% to 104%, the C.V was between 6.2% to 21%. The pre-treatment method of samples and the chromatographic conditions of the analysis were critically examined in experiment, the above method were confirmed for its easier, exacter, faster and being better purification effect.
Studies on residual degradation dynamics of endosulfan in cotton and soil were according to spraying once, sampling time after time. The results showed: in ShiJiaZhuang, the degradation curve equations of endosulfan in cotton and soil in different term and the degradation dynamics were: C_T=33.6e~(-0.117T) and C_T=2.38e~(-0.0348T). The correlation coefficients were -0.9491 and -0.8368 and the half-lives were 5.92 and 19.92 days. In ChangSha, the degradation dynamics were: C_T=31.3e~(-0.116T) and C_T=9.20e~(-0.0460T). The correlation coefficients were -0.8597 and -0.9521. The half-lives were 5.99 and 15.07 days. Residual degradation dynamics of endosulfan in different term in cotton and soil were all accorded with C=Coe-kt and all had high relativity. The degradation speed of endosulfan in cotton is fast than in soil and the degradation speed in ShiJiaZhuang is different from ChangSha.
The final residues experiment: cotton were treated three and four times and interval of 14 days with the dosages of 945a.i.g/hm~2 and 1890a.i.g/hm~2 during the growing season of cotton. The application after harvest interval was 7 days. The result of final residues of endosulfan showed: in ShiJiaZhuang, the final residues of endosulfan in cotton were 0.492mg/kg to 13.2mg/kg. In cottonseed was not detectable, in soil was 0.196mg/kg to 0.851mg/kg. In ChangSha, the final residues of endosulfan in cotton was 0.421mg/kg to 1.58mg/kg, in cottonseed was not detectable,in soil was 0.114mg/kg to 0.258mg/kg. According to these data: there were positive relation between residue and consistence as well as times sprays for, the high of the concentration in usage and the more times of applying, the residual are more higher, and negative relation residue and the days from last application to harvest and the residual in cotton is above soil.
Refer to FAO/WTO MRL endosulfan in cottonseed that MRL is 1.0mg/kg and our government prescribe the MRL in cottonseed is 1.0mg/kg. The result of the final residual showed: 35% endosulfan EC used on cotton is safe. The dosage of endosulfan applied on cotton is 945a.i.g/hm~2 to 1890a.i.g/hm~2 are all below limit standard and endosulfan in cottonseed was not detectable. So based on the result of the trial, we suggest that the dosage of endosulfan applied on cotton be 945a.i.g/hm~2 or 1890a.i.g/hm~2 and three or four times during the cotton growing period and MRL be 1.0mg/kg in cottonseed. The safe interval period should be 14 days. In this safe period, endosulfan is safe to environment and when harvest that the residual of cottonseed was not detectable and suitable the residual standard.
硫丹的残留分析方法研究:以棉叶、棉籽和土壤为研究材料,采用气相色谱法,通过实验对不同残留分析方法进行了比较。结果表明:棉叶和棉籽样品用乙腈、氟罗里硅土固相萃取小柱净化,后经气相色谱检测。在棉叶、棉籽中的添加回收率分别为82.0%～95.7%和82.9%～95.6%,变异系数分别为6.9%～13%和3.2%～20%。土壤样品用丙酮提取,石油醚液-液萃取,旋转蒸发仪浓缩后,经氟罗里硅土固相萃取小柱净化,后经气相色谱仪检测,硫丹在土壤中的添加回收率在87.6%～104%之间,变异系数在6.2%～21%之间。在实验过程中,对样品前处理和色谱分离条件进行了详细的研究和优化,最终确证以上方法简便、准确、快速、净化效果好,满足农药残留分析的要求。
消解动态试验:按照一次施药多次采样的方法对35%硫丹乳油在棉叶和土壤中的消解动态进行研究。结果表明:在石家庄市,硫丹在棉叶中的消解动态方程为C_T=33.6e~(-0.117T),在土壤中的消解动态方程为C_T=2.38e~(-0.0348T),相关系数分别为-0.9491和-0.8368,消解半衰期分别为5.92d和19.92d。在长沙市,硫丹在棉叶和土壤中的消解动态方程分别为C_T=31.3e~(-0.116T)和C_T=9.20e~(-0.0460T),相关系数分别为-0.8597和-0.9521,消解半衰期分别为5.99d和15.07d。硫丹在不同时期在棉叶和土壤中的消解动态均符合一级动力学反应模式,都具有较高的相关性。硫丹在棉叶中的消解速率较土壤中快,石家庄地区的消解速率也不同于长沙地区。
最终残留试验:在945a.i.g/hm~2、1890a.i.g/hm~2的施药水平下,施药3次、4次,每次施药间隔期为14d,施药后距采收间隔期为7d,最终残留试验结果表明:在石家庄市,硫丹在棉叶中的最终残留量为0.492-13.2mg/kg,在土壤中的最终残留量为0.196-0.851mg/kg。在长沙市,硫丹在棉叶中的最终残留量为0.421-1.58mg/kg,在土壤中的最终残留量为0.114-0.258mg/kg,在棉籽中两地均未检出有硫丹残留,从数据分析来看:硫丹的最终残留量与施药浓度、施药次数呈正相关,施药浓度越高,施药次数越多,残留量越高;与采收间隔期呈负相关,且棉叶中的残留量高于土壤中。
联合国粮农组织(FAO/WTO)制定其标准为:硫丹在棉花籽中的最高残留限量(MRL)为1.0mg/kg。我国的推荐性国家标准:硫丹在棉籽中的最高残留限量为1.0mg/kg。由硫丹在棉花中的最终残留试验结果,我们可以看出35%硫丹乳油用于棉花害虫防治的过程中,按推荐剂量945a.i.g/hm~2和加倍剂量1890a.i.g/hm~2使用,其残留量均低于限定标准,在棉籽中均没有检测出硫丹的残留。因此,根据本研究,暂建议在棉花的生长时期,35%硫丹乳油使用剂量为推荐剂量945a.i.g/hm~2和加倍剂量1890a.i.g/hm~2,施药3到4次且最大残留限量为1.0mg/kg,在棉花中的安全间隔期为14d,在此安全间隔期内施药对环境安全,收获棉花时其棉籽中未检出有硫丹残留,其残留量满足残留标准要求,是安全的。
As a high efficiency Organochlorine insecticide, endosulfan is only pesticide that use in prevention cotton bollworm and cotton aphid. In agricultural production, endosulfan will take a key role in the future. In order to use endosulfan in science way, evaluate the residue level in cotton and affect in soil. In this paper, one-year residue-assignment of endosulfan was carried out in different Places. A method was studied for the analysis of endosulfan residue in cotton, cottonseed and soil and 35% endosulfan EC had been recommended to control cotton bollworm in cotton according to the results of field experiment.
Residue analysis methods research on endosulfan, gas chromatography method has been used in cotton、cottonseed and soil, different methods have been compared by experiments. The results show, cotton and cottonseed sample were extracted with acetonitrile, after the extract was cleaned up with a Florisil column, endosulfan was analyzed by GC, the average recoveries of cotton ranged from 82.0% to 95.7%, the C.V was between 6.9% to 13%. The average recoveries of cottonseed ranged from 82.9% to 95.6%, the C.V was 3.2% to 20%. Soil sample was extracted with acetone, LLE by petroleum ether, the average recoveries of soil ranged from 87.6% to 104%, the C.V was between 6.2% to 21%. The pre-treatment method of samples and the chromatographic conditions of the analysis were critically examined in experiment, the above method were confirmed for its easier, exacter, faster and being better purification effect.
Studies on residual degradation dynamics of endosulfan in cotton and soil were according to spraying once, sampling time after time. The results showed: in ShiJiaZhuang, the degradation curve equations of endosulfan in cotton and soil in different term and the degradation dynamics were: C_T=33.6e~(-0.117T) and C_T=2.38e~(-0.0348T). The correlation coefficients were -0.9491 and -0.8368 and the half-lives were 5.92 and 19.92 days. In ChangSha, the degradation dynamics were: C_T=31.3e~(-0.116T) and C_T=9.20e~(-0.0460T). The correlation coefficients were -0.8597 and -0.9521. The half-lives were 5.99 and 15.07 days. Residual degradation dynamics of endosulfan in different term in cotton and soil were all accorded with C=Coe-kt and all had high relativity. The degradation speed of endosulfan in cotton is fast than in soil and the degradation speed in ShiJiaZhuang is different from ChangSha.
The final residues experiment: cotton were treated three and four times and interval of 14 days with the dosages of 945a.i.g/hm~2 and 1890a.i.g/hm~2 during the growing season of cotton. The application after harvest interval was 7 days. The result of final residues of endosulfan showed: in ShiJiaZhuang, the final residues of endosulfan in cotton were 0.492mg/kg to 13.2mg/kg. In cottonseed was not detectable, in soil was 0.196mg/kg to 0.851mg/kg. In ChangSha, the final residues of endosulfan in cotton was 0.421mg/kg to 1.58mg/kg, in cottonseed was not detectable,in soil was 0.114mg/kg to 0.258mg/kg. According to these data: there were positive relation between residue and consistence as well as times sprays for, the high of the concentration in usage and the more times of applying, the residual are more higher, and negative relation residue and the days from last application to harvest and the residual in cotton is above soil.
Refer to FAO/WTO MRL endosulfan in cottonseed that MRL is 1.0mg/kg and our government prescribe the MRL in cottonseed is 1.0mg/kg. The result of the final residual showed: 35% endosulfan EC used on cotton is safe. The dosage of endosulfan applied on cotton is 945a.i.g/hm~2 to 1890a.i.g/hm~2 are all below limit standard and endosulfan in cottonseed was not detectable. So based on the result of the trial, we suggest that the dosage of endosulfan applied on cotton be 945a.i.g/hm~2 or 1890a.i.g/hm~2 and three or four times during the cotton growing period and MRL be 1.0mg/kg in cottonseed. The safe interval period should be 14 days. In this safe period, endosulfan is safe to environment and when harvest that the residual of cottonseed was not detectable and suitable the residual standard.
引文
[1]赵善欢.植物化学保护(第三版)[M].北京,中国农业出版社,2001
[2]袁增雯,张一宾.农药与环境[J].化学世界.2000,22-24
[3]屠豫钦.农药使用技术标准化[M].北京,中国标准出版社,2001,1-4
[4]林玉锁,龚瑞忠,朱忠林.农药与生态环境保护[M].北京,化学工业出版社,2000
[5]卢植新.生态农业与可持续发展.首届中国可持续消费与生产国际论坛暨中国环境科学学会 2003 年学术年会论文汇编.长沙,2003:356-359
[6]山本出,深见顺一主编.农药的设计与开发指南(第二册)农药的代谢降解与毒理[M].北京,化学工业出版社,1991
[7]江希流,华小梅.加强农药的环境管理刻不容缓[J].农村生态环境,2000,16(2):35-38
[8]单正军,朱忠林,华小梅.我国农药环境污染及管理现状[J].环境保护,1997,07:40-43
[9]W.D.冈吉(美).土壤和水中的农药[M].北京,科学出版社,1985
[10]蔡道基.农药环境毒理学研究[M].北京,中国环境科学出版社,1999
[11]秦富,王秀清,辛贤等.欧美食品安全体系研究[M].北京,中国农业出版社,2003
[12]日本环境厅水质保全局土壤农药课编.许泳峰译.农药污染[M].北京,农业出版社,1983
[13]莫汉宏.农药环境化学行为论文集[M].北京,中国科学技术出版社,1994
[14]中国预防医学科学院标准处.食品卫生国家标准汇编.北京:中国标准出版社,1997.
[15]吴坤.实用食品毒理学[M].哈尔滨:黑龙江科学技术出版社,1997.
[16]Antonio Abad Maria Jose Moreno,Angel Montoya. Development of monoclonal antibody-based immunassays to the n-methylcarbamate pesticide carbofuran. Agirc Food Chem,1999,47(6):2475-2485
[17]黄伟坤等.食品检验与分析[M].北京:中国轻工业出版社,1989
[18]庄无忌.各国食品和饲料中农药兽药残留限量大全[M].北京,中国对外经济贸易出版社,1995
[19]乔雄梧.国外食品中农药残留监测概况[J].农药科学与管理.2000,21(2):11-15
[20]李本昌.农药残留量实用检测方法手册(第一卷)[M].北京,中国农业科技出版社,1995
[21]樊德方.农药残留分析与检测[M].上海,上海科学技术出版社,1982
[22]Hua XM, Jiang X L.The Production and application of pesticides and their pollution in environment in China [J].Environmental Protection , 1999 ,26(9):23-25 (in Chinese)
[23]Liu XM, Peng P. Huang WL , et al.Environmental behavior and research progress of HCHs [J].Agricultural Environment and Development , 2001,(2):38-40 (in Chinese)
[24]Frank Wania , Donald Mackay. An approach to modeling the global distribution of toxaphene : a discussion of feasibility and desirability[J ] . Chemosphere , 1993 ,27 (10):2079-2094
[25]Jones KC Voogt Pde.Persistent organic polluantants(POPs):state of the science[J].Environmental Pollution,1999,100:209-221
[26]赵云峰.我国居民膳食中农药残留的研究[J].中华流行病学杂志,2003,4(8):661
[27]Shi YJ , Lu YL , Ren H C , et al . Recent progress in scientific research on persistent organic pollutants[J].World Sci -Tech R &D , 2003 , 25(2) :73-78 (in Chinese)
[28]Derek C.G Muir, Barr R Hobden and Mark R. Servos. Bioconcentration of pyrethroidinsecticides and DDT rainbow trout:uptake,depuration,and effect of dissolved organic carbon[J]. Aquatic Toxicology 1994,29(3-4):223-240
[29]郁亚娟,黄宏,王斌等.淮河(江苏段)水体有机氯农药的污染水平[J].环境化学.2004,23(5):568-572
[30]张祖麟,洪华生,陈伟琪等.闽江口水、间隙水和沉积物中有机氯农药的含量[J].环境科学.2003,24(l):117-120
[31]文峰,范莉,尹辉等.山民江成都段有机物污染调查[J].环境监测管理与技术.2005,17(3):22-25
[32]杨嘉漠,王赞,苏青青.长江武汉段水体悬浮物中有机氯农药的残留状况[J].环境科学研究.2004,17(6):27-34
[33]杨清书,麦碧娴,傅家漠等.珠江干流河口水体有机氯农药的研究[J].中国环境科学.2005,25(增刊):47-51
[34]张秀芳,董晓丽.辽河中下游水体中有机氯农药的残留调查[J].大连轻工业学院学报.2002,21(2):102-104
[35]梁洪军,高志贤.薄层色谱扫描测定粮食、蔬菜中有机氯农药残留量的方法研究[J].农药.1994,12(4):283-285
[36] 薛秀玲,袁东星,吴翠琴等.福建沿海养殖贝类中农药残留的含量及来源分析[J].海洋环境科学.2004,23(2):40-42
[37]宋平顺,卫玉玲,丁永辉等.甘肃省人工栽培中药材有机氯农药残留量分析[J].兰州医学院学报.2003,29(4):11-12
[38]Iwata Hisato,Tanabe Shilisuke. Distribution of persistent organochlorineds in the oleanic air and surface seawater and the role of ocean on their global transport and fate[J].Environ.SciTechnol.1993.27(6):1080-1098
[39]姚子伟,江桂斌,蔡亚歧等.北极地区表层海水中持久性有机污染物和重金属污染的现状[J].科学通报.2002,47(15):1196-1200
[40]刘秀芬,劳文剑,毕新慧等.南极鲍鱼样品中有机氯农药的测定[J].分析化学.2002,30(9):1035-1037
[41]Yu H , Zhu Z , Zhao X, et al . Levels of organochlorine pesticides in beijing human milk [J]. Bulletin of Environmental Contamination and Toxicology , 2003 ,70:193-197
[42]Zhang Z L , Hong H S , Khalid M, et al . The trends and characteristics of organochlorines pollution in surface sediments of xiamen western Bay [J].Acta Scientiae Circumstantiae , 2000 , 20(6):731-735 (in Chinese)
[43]Yao Z W, Jiang G B , Cai Y Q , et al . The pollution of persistent organic pollutants and heavy metals in surface waters of artic poleregion[J ] . Chinese Science Bulletin , 2002 ,47(15):1196-1200 (inChinese)
[44]Zhao L , Ma Y J , Analysis of the status of organochlorinated pesticides in the agricultural environment [J].Agricultural Environment and Development , 2001 (1):7 -39 (in Chinese)
[45]Ron van der Oost,Antoon Opperhuizen,Karel Satumalay et al. Biomonitoring aquatic pollution withferal eel(Anguilla anguilla)1.Bioaccumulation:biota-sediment ratios of PCBs , OCPs , PCDDs and PCDFs[J].Aquatic Toxicology 1996.35(l):21-46
[46]朱忠林.农药污染与人体健康[J].环境保护.1994.6:45-47
[47]张玲,祁玉峰.控制农药残留保障农产品质量安全[J].河南农业科学,2002(10):27-28
[48]田子华.国内外农药残留限量标准分析及农产品质量建设应对措施探讨[J].江苏农业科学,2003(5):11-14
[49]黄琼辉.蔬菜农药残留现状及治理对策[J].福建农业科技,2002,5:43-44
[50]张浩,王岩,途忠斌等.莎阔丹除草剂在稻田环境中的残留动态研究[J].农药学学报,2003,5(3):64-68
[51]刘干开,朱国念.高氰戊菊醋在小麦上的残留及消解动态[J].浙江农业大学学报,1994,20(4):359-362
[52]冯世德,王波,曲红杰. 农药残留分析技术进展概述[J].黑龙江农业科学,2005,(3):27-29
[53]Nilsson U J. Solid-phase extraction for combinatorial libraries[J]. J Chromatography A, 2000, 885(1-2):305-319
[54]Carson M C. Ion-pair solid-phase extraction[J]. J Chromatography A, 2000, 885(1-2):343-350
[55]Fritz J S, Dumont P J, Schmidt L W. Methods and materials for solid-phase extraction[J]. J Chromatography A, 1995, 691(1-2):133-140
[56]付颖,王常波,叶非等.固相微萃取技术在农药残留分析中的应用[J]. 理化检验-化学分册,2006,42(10):865-870
[57]Belardi R G, Pawliszyn J.Water Pollution Res J Can[J].1989 ,24 (1):179-191
[58]仲维科,郝戮,樊耀波等.食品农药残留分析进展[J].分析化学,2000,28(7):904-910
[59]吴瑛,袁守亮,任丽萍等. 农业环境中农药残留的快速检测法—分子印迹技术[J].安徽农业科学,2007,35(17):5040-5041,5043
[60]陈华才.简论农药残留量检测中的样品前处理技术[J].中国计量学院学报,2002,13(3):240-244
[61]Snnino A,Mambriani P,Bandini M,el al .Multiresidue method for determination of organophosphorus insecticide residues in fatty Processed foods by gel permeation chromatography[J].AOAC Int,1995,78(6):1502-1512.
[62]孙毓庆.现代色谱法及其在药物分析中的应用[M].北京科学出版社.2005
[63]FJ. Arrebola, J.L. Martynez Vidal, M, Mateu-Sanchez, et al. Determination of 81 multiclass pesticides in fresh foodstuffs by a single injection analysis using gas chromatography--chemical ionization and electron ionizationion tandem mass spectrometry[J].Analytica Chimica Acta 2003,484(2):167-180
[64]Steve Schachterle,Robert D.Brittain,John D.Mills Analysis of pesticide residues in food using gas chromatography一tandem mass spectrometry with a benchtop ion trap mass spectrometer[J].Joumal of ChromatographyA.1994,683(l):185-193
[65]张娟,李方实.高效液相色谱法测定土壤中异丙隆及其降解产物对异丙基苯胺[J].农业环境科学学报,2005,(5):170-172
[66]张浩,王岩,途忠斌等.反相高效液相法检测土壤中的咪草烟[J].农业环境科学保护,2001,(4):80-82
[67]渠锋,莫汉宏,安凤春.反相高效液相色谱法检测水、土壤中的除草剂[J].农业环境保护,1999,18(2):79-80
[68]慕卫,刘峰,王金信.反相高效液相色谱法同时测定土壤中莠去津、氰草津残留量方法研究[J].分析化学,1998,26(5):571-573
[69]杨舰.高效液相色谱/质谱联用仪在农药全分析中的应用[J].环境化学,2001,20(4):408-409
[70]金军,赵国栋,崔云.高效液相色谱/大气压电离质谱(HPLC/A PIMS)在农药残留分析中的应用[J].化学通报,1999,(6):25-29
[71]Belmonte Vega A,Garride Frenich A,Martinez Vidal J L. Monitoring of Pesticides in agricultural water and soil samples from Andalusia by liquid chromatography coupled to mass spectrometry[J].Analytica Chimica Acta,2005,538(1-2):117-127
[72]蔺岩,岳海燕.用快速薄层法同时测定食品中含硫有机磷农药和敌敌畏、敌百虫[J].食品开发与研究,2004,25(2):121-123
[73]Sanchez-Camazano M,Sanchez-Martin M J,Poveda E,Iglesias-Jimnez E.Study of the effect of exogenous organic matter on the mobility of pesticides in soil using soil thin-layer chromatography[J]. Journal of ChromatographyA,1996,754(1-2):279-284
[74]刘曙照,钱传范.九十年代农药残留分析新技术[J].农药,1998,37(6):11-13
[75]费新平,王立世,张水锋等.毛细管电泳-安培检测法对甲基对硫磷、对硫磷、西维因和速灭威农药残留的测定研究[J].分析测学报,2004,23(5):70-73
[76]王艳,高志贤,戴树桂.农药残留免疫分析方法及其研究进展田[J].农业环境科学学报,2003,22(l):125-127
[77]杨依军,王勇,杨秀荣等.免疫分析法在农药残留分析中的应用[J].华北农学报,2001,16(4):119-124
[78]邓安平,杨红,Franek Milan.酶联免疫吸附分析法测定上壤试样中的阿特拉津[J].分析化学,1999,27(6):657-660
[79]黄德智,岳永德,汤锋. 农药残留分析的研究进展[J].安徽农业科学,2002,30(4):523-526,541
[80]刘乾开,朱国念.新编农药使用手册(第二版)[M].上海:上海科学技术出版社,1999.309.
[81]Wilkes,P.S. Gas-chromatographic-mass spectrometric confirmation of endosulfan and endosulfan sulfate in apples and carrots. J. Assoc.off. Anal.Chem.1981,64,1208-1210.
[82]于金凤. 硫丹对抗性棉铃虫某些生物学特性的影响[J].农药科学与管理.1999,20(2):18-19
[83]化学工业部农药情报中心站,国外农药品种手册(三)[M].1981:49-53
[84]Forrester,N.W.Australian Cotton Grower,1989,10(3):62-69
[85]王开运等.硫丹对抗性棉蚜、棉铃虫的防治效果及应用展望[J].农药,1993,32(2):2-4
[86]王金信等. 几类农药防治抗性棉蚜的药效评价[J].农药.1997,36(1):8-9
[87]王成菊.丙锈磷等对棉铃虫不同龄期的作用特点研究[J].农药,1996,35(1):30-33
[88]国家环境保护局.化学农药环境安全评价试验准则[M].1989
[89]莫汉宏,安风春等.农药在农田生态环境中的持久性及残留研究方法[J].农药环境化学行为论文集[M],1994,24-29
[90]王守玉.新疆棉铃虫对溴氰菊酯和硫丹抗性的生化机理研究[J].昆虫学报.2000(增刊):44-50
[91]于金凤.棉蚜对不同类型杀虫剂的抗性发生规律[J].山东农业大学学报.1997,28(3):315-319
[92]丁中.硫丹合成工艺的研究[J].农药.2001,40(6):18-21
[93]郜红建.有机氯农药在南京市郊蔬菜中的生物富集与质量安全[J].环境科学报.2005,25(1):90-93
[94]张百臻.长期使用硫丹可能对人身健康和生态环境造成严重危害. 农药科学与管理.2002,23(5):35
[95]陈惠.印度国家腰果研究中心提议禁用硫丹防治虫害.Tcashew.2000,16(4):2
[96]杨伟华等.棉花产品中农药残留情况初步研究[J].中国棉花,研究简报.2006 , 33 (3):12-15
[97]郭明等. 赛丹在棉花和土壤中的残留动态研究[J].农业环境保护,2001,20(4):243-245
[98]Abel, C. A., and G. L. Snodgrass. 2004. Tarnished plant bug development in Teld corn. Proceedings of the Beltwide Cotton Production and Research Conference, National Cotton Council, January, San Antonio, TX.
[99]Tugwell, P., S.C. Young, Jr., B. A. Dumas, and J. R. Phillips. 1976. Plant bugs in cotton: Importance of infestation time, types of cotton injury, and signiTcance of wild hosts near cotton. University of Arkansas Agricultural Experiment Station, U. of Arkansas Ag. Expr. Sta. Rpt. 227. 24 pp. Received for publication 28 October 2005; accepted 21 June 2006.
[100]Pankey, J. H., B. R. Leonard, J. B. Graves, and E. Burris. 1996. Toxicity of acephate, cypermethrin, and oxamyl to tarnished plant bugs in vial bioassays and cage studies on cotton. Proceedings of the Beltwide Cotton Production Research Conference, National Cotton Council, January, Nashville, TN
[101]张骏.食品中硫丹残留的酶联免疫吸附检测方法的研究. 中国食品科技学会第四届年会论文
[102]韩熹莱主编.农药概论.北京农业大学出版社,1995
[103]Funari E,Donati L,Sandroni D,et al.Pesticide levels in groundwater:values and limitations of monitoring[A]//Vighi M,Funari E,ed.Pesticide risk in groundwater[M].Boca Raton:CPC Pres,1995:3-44
[104]欧晓明等.农药结合残留的研究现状及问题[J].农药,2006,45(10):660-663
[105]曹爱华,徐光军,冯涛等.赛丹(endosulfan)在烟草和土壤中的残留研究[J].中国烟草科学,1999,3:40-44
[106]明九雪,钱传范,申继忠.硫丹(endosulfan)在苹果和土壤中的残留动态研究[J].中国农业大学学报,1998,3(3):95-100
[107]吕萧,聂燕,陈子雷等. EL ISA快速测定小麦中硫丹及硫丹硫酸酯[J].中国粮油学报,2006,21(5):41-45
[108]吕萧,任凤山,王文博等.茶叶中硫丹及硫丹硫酸酯的ELISA 快速测定[J].茶叶科学,2006,26(2):141-146
[109]吴景平,司宗兴.硫丹的高效液相色谱分析[J].农药科学与管理,1995,1:12-13
[110]刘小松,黄大新,卢声宇. 毛细管气相色谱法测定南美白对虾中硫丹的残留量[J].色谱,2007,25(3):392-394
[111]陈化才.赛丹在茶叶中的残留降解动态研究[J].农药,2000,39(3):25-27
[112]谭道朝, 蒋廷福. 农药残留分析样品前处理技术发展概述[J].广西植保,2007,20(2):16-20
[113]陈万义,薛振祥,王能武主编.新农药研究与开发[M].北京,化学工业出版社,1995,115-116
[114]农业部农药检定所.农业部行业标准 NY/T 788-2004《农药残留试验准则》[S].北京:中国标准出版社,2004
[115]张韩杰,闰艳春.农药残留及微生物在农药降解中的应用与展望[J].湖北植保 2004,31-35
[116] Ghadiri H, Rose C.W.Degradation of endosulfan in a clay soil from cotton farms of westrn Queensland. Journal of Environmental management, 2001, 62:155-169.
[2]袁增雯,张一宾.农药与环境[J].化学世界.2000,22-24
[3]屠豫钦.农药使用技术标准化[M].北京,中国标准出版社,2001,1-4
[4]林玉锁,龚瑞忠,朱忠林.农药与生态环境保护[M].北京,化学工业出版社,2000
[5]卢植新.生态农业与可持续发展.首届中国可持续消费与生产国际论坛暨中国环境科学学会 2003 年学术年会论文汇编.长沙,2003:356-359
[6]山本出,深见顺一主编.农药的设计与开发指南(第二册)农药的代谢降解与毒理[M].北京,化学工业出版社,1991
[7]江希流,华小梅.加强农药的环境管理刻不容缓[J].农村生态环境,2000,16(2):35-38
[8]单正军,朱忠林,华小梅.我国农药环境污染及管理现状[J].环境保护,1997,07:40-43
[9]W.D.冈吉(美).土壤和水中的农药[M].北京,科学出版社,1985
[10]蔡道基.农药环境毒理学研究[M].北京,中国环境科学出版社,1999
[11]秦富,王秀清,辛贤等.欧美食品安全体系研究[M].北京,中国农业出版社,2003
[12]日本环境厅水质保全局土壤农药课编.许泳峰译.农药污染[M].北京,农业出版社,1983
[13]莫汉宏.农药环境化学行为论文集[M].北京,中国科学技术出版社,1994
[14]中国预防医学科学院标准处.食品卫生国家标准汇编.北京:中国标准出版社,1997.
[15]吴坤.实用食品毒理学[M].哈尔滨:黑龙江科学技术出版社,1997.
[16]Antonio Abad Maria Jose Moreno,Angel Montoya. Development of monoclonal antibody-based immunassays to the n-methylcarbamate pesticide carbofuran. Agirc Food Chem,1999,47(6):2475-2485
[17]黄伟坤等.食品检验与分析[M].北京:中国轻工业出版社,1989
[18]庄无忌.各国食品和饲料中农药兽药残留限量大全[M].北京,中国对外经济贸易出版社,1995
[19]乔雄梧.国外食品中农药残留监测概况[J].农药科学与管理.2000,21(2):11-15
[20]李本昌.农药残留量实用检测方法手册(第一卷)[M].北京,中国农业科技出版社,1995
[21]樊德方.农药残留分析与检测[M].上海,上海科学技术出版社,1982
[22]Hua XM, Jiang X L.The Production and application of pesticides and their pollution in environment in China [J].Environmental Protection , 1999 ,26(9):23-25 (in Chinese)
[23]Liu XM, Peng P. Huang WL , et al.Environmental behavior and research progress of HCHs [J].Agricultural Environment and Development , 2001,(2):38-40 (in Chinese)
[24]Frank Wania , Donald Mackay. An approach to modeling the global distribution of toxaphene : a discussion of feasibility and desirability[J ] . Chemosphere , 1993 ,27 (10):2079-2094
[25]Jones KC Voogt Pde.Persistent organic polluantants(POPs):state of the science[J].Environmental Pollution,1999,100:209-221
[26]赵云峰.我国居民膳食中农药残留的研究[J].中华流行病学杂志,2003,4(8):661
[27]Shi YJ , Lu YL , Ren H C , et al . Recent progress in scientific research on persistent organic pollutants[J].World Sci -Tech R &D , 2003 , 25(2) :73-78 (in Chinese)
[28]Derek C.G Muir, Barr R Hobden and Mark R. Servos. Bioconcentration of pyrethroidinsecticides and DDT rainbow trout:uptake,depuration,and effect of dissolved organic carbon[J]. Aquatic Toxicology 1994,29(3-4):223-240
[29]郁亚娟,黄宏,王斌等.淮河(江苏段)水体有机氯农药的污染水平[J].环境化学.2004,23(5):568-572
[30]张祖麟,洪华生,陈伟琪等.闽江口水、间隙水和沉积物中有机氯农药的含量[J].环境科学.2003,24(l):117-120
[31]文峰,范莉,尹辉等.山民江成都段有机物污染调查[J].环境监测管理与技术.2005,17(3):22-25
[32]杨嘉漠,王赞,苏青青.长江武汉段水体悬浮物中有机氯农药的残留状况[J].环境科学研究.2004,17(6):27-34
[33]杨清书,麦碧娴,傅家漠等.珠江干流河口水体有机氯农药的研究[J].中国环境科学.2005,25(增刊):47-51
[34]张秀芳,董晓丽.辽河中下游水体中有机氯农药的残留调查[J].大连轻工业学院学报.2002,21(2):102-104
[35]梁洪军,高志贤.薄层色谱扫描测定粮食、蔬菜中有机氯农药残留量的方法研究[J].农药.1994,12(4):283-285
[36] 薛秀玲,袁东星,吴翠琴等.福建沿海养殖贝类中农药残留的含量及来源分析[J].海洋环境科学.2004,23(2):40-42
[37]宋平顺,卫玉玲,丁永辉等.甘肃省人工栽培中药材有机氯农药残留量分析[J].兰州医学院学报.2003,29(4):11-12
[38]Iwata Hisato,Tanabe Shilisuke. Distribution of persistent organochlorineds in the oleanic air and surface seawater and the role of ocean on their global transport and fate[J].Environ.SciTechnol.1993.27(6):1080-1098
[39]姚子伟,江桂斌,蔡亚歧等.北极地区表层海水中持久性有机污染物和重金属污染的现状[J].科学通报.2002,47(15):1196-1200
[40]刘秀芬,劳文剑,毕新慧等.南极鲍鱼样品中有机氯农药的测定[J].分析化学.2002,30(9):1035-1037
[41]Yu H , Zhu Z , Zhao X, et al . Levels of organochlorine pesticides in beijing human milk [J]. Bulletin of Environmental Contamination and Toxicology , 2003 ,70:193-197
[42]Zhang Z L , Hong H S , Khalid M, et al . The trends and characteristics of organochlorines pollution in surface sediments of xiamen western Bay [J].Acta Scientiae Circumstantiae , 2000 , 20(6):731-735 (in Chinese)
[43]Yao Z W, Jiang G B , Cai Y Q , et al . The pollution of persistent organic pollutants and heavy metals in surface waters of artic poleregion[J ] . Chinese Science Bulletin , 2002 ,47(15):1196-1200 (inChinese)
[44]Zhao L , Ma Y J , Analysis of the status of organochlorinated pesticides in the agricultural environment [J].Agricultural Environment and Development , 2001 (1):7 -39 (in Chinese)
[45]Ron van der Oost,Antoon Opperhuizen,Karel Satumalay et al. Biomonitoring aquatic pollution withferal eel(Anguilla anguilla)1.Bioaccumulation:biota-sediment ratios of PCBs , OCPs , PCDDs and PCDFs[J].Aquatic Toxicology 1996.35(l):21-46
[46]朱忠林.农药污染与人体健康[J].环境保护.1994.6:45-47
[47]张玲,祁玉峰.控制农药残留保障农产品质量安全[J].河南农业科学,2002(10):27-28
[48]田子华.国内外农药残留限量标准分析及农产品质量建设应对措施探讨[J].江苏农业科学,2003(5):11-14
[49]黄琼辉.蔬菜农药残留现状及治理对策[J].福建农业科技,2002,5:43-44
[50]张浩,王岩,途忠斌等.莎阔丹除草剂在稻田环境中的残留动态研究[J].农药学学报,2003,5(3):64-68
[51]刘干开,朱国念.高氰戊菊醋在小麦上的残留及消解动态[J].浙江农业大学学报,1994,20(4):359-362
[52]冯世德,王波,曲红杰. 农药残留分析技术进展概述[J].黑龙江农业科学,2005,(3):27-29
[53]Nilsson U J. Solid-phase extraction for combinatorial libraries[J]. J Chromatography A, 2000, 885(1-2):305-319
[54]Carson M C. Ion-pair solid-phase extraction[J]. J Chromatography A, 2000, 885(1-2):343-350
[55]Fritz J S, Dumont P J, Schmidt L W. Methods and materials for solid-phase extraction[J]. J Chromatography A, 1995, 691(1-2):133-140
[56]付颖,王常波,叶非等.固相微萃取技术在农药残留分析中的应用[J]. 理化检验-化学分册,2006,42(10):865-870
[57]Belardi R G, Pawliszyn J.Water Pollution Res J Can[J].1989 ,24 (1):179-191
[58]仲维科,郝戮,樊耀波等.食品农药残留分析进展[J].分析化学,2000,28(7):904-910
[59]吴瑛,袁守亮,任丽萍等. 农业环境中农药残留的快速检测法—分子印迹技术[J].安徽农业科学,2007,35(17):5040-5041,5043
[60]陈华才.简论农药残留量检测中的样品前处理技术[J].中国计量学院学报,2002,13(3):240-244
[61]Snnino A,Mambriani P,Bandini M,el al .Multiresidue method for determination of organophosphorus insecticide residues in fatty Processed foods by gel permeation chromatography[J].AOAC Int,1995,78(6):1502-1512.
[62]孙毓庆.现代色谱法及其在药物分析中的应用[M].北京科学出版社.2005
[63]FJ. Arrebola, J.L. Martynez Vidal, M, Mateu-Sanchez, et al. Determination of 81 multiclass pesticides in fresh foodstuffs by a single injection analysis using gas chromatography--chemical ionization and electron ionizationion tandem mass spectrometry[J].Analytica Chimica Acta 2003,484(2):167-180
[64]Steve Schachterle,Robert D.Brittain,John D.Mills Analysis of pesticide residues in food using gas chromatography一tandem mass spectrometry with a benchtop ion trap mass spectrometer[J].Joumal of ChromatographyA.1994,683(l):185-193
[65]张娟,李方实.高效液相色谱法测定土壤中异丙隆及其降解产物对异丙基苯胺[J].农业环境科学学报,2005,(5):170-172
[66]张浩,王岩,途忠斌等.反相高效液相法检测土壤中的咪草烟[J].农业环境科学保护,2001,(4):80-82
[67]渠锋,莫汉宏,安凤春.反相高效液相色谱法检测水、土壤中的除草剂[J].农业环境保护,1999,18(2):79-80
[68]慕卫,刘峰,王金信.反相高效液相色谱法同时测定土壤中莠去津、氰草津残留量方法研究[J].分析化学,1998,26(5):571-573
[69]杨舰.高效液相色谱/质谱联用仪在农药全分析中的应用[J].环境化学,2001,20(4):408-409
[70]金军,赵国栋,崔云.高效液相色谱/大气压电离质谱(HPLC/A PIMS)在农药残留分析中的应用[J].化学通报,1999,(6):25-29
[71]Belmonte Vega A,Garride Frenich A,Martinez Vidal J L. Monitoring of Pesticides in agricultural water and soil samples from Andalusia by liquid chromatography coupled to mass spectrometry[J].Analytica Chimica Acta,2005,538(1-2):117-127
[72]蔺岩,岳海燕.用快速薄层法同时测定食品中含硫有机磷农药和敌敌畏、敌百虫[J].食品开发与研究,2004,25(2):121-123
[73]Sanchez-Camazano M,Sanchez-Martin M J,Poveda E,Iglesias-Jimnez E.Study of the effect of exogenous organic matter on the mobility of pesticides in soil using soil thin-layer chromatography[J]. Journal of ChromatographyA,1996,754(1-2):279-284
[74]刘曙照,钱传范.九十年代农药残留分析新技术[J].农药,1998,37(6):11-13
[75]费新平,王立世,张水锋等.毛细管电泳-安培检测法对甲基对硫磷、对硫磷、西维因和速灭威农药残留的测定研究[J].分析测学报,2004,23(5):70-73
[76]王艳,高志贤,戴树桂.农药残留免疫分析方法及其研究进展田[J].农业环境科学学报,2003,22(l):125-127
[77]杨依军,王勇,杨秀荣等.免疫分析法在农药残留分析中的应用[J].华北农学报,2001,16(4):119-124
[78]邓安平,杨红,Franek Milan.酶联免疫吸附分析法测定上壤试样中的阿特拉津[J].分析化学,1999,27(6):657-660
[79]黄德智,岳永德,汤锋. 农药残留分析的研究进展[J].安徽农业科学,2002,30(4):523-526,541
[80]刘乾开,朱国念.新编农药使用手册(第二版)[M].上海:上海科学技术出版社,1999.309.
[81]Wilkes,P.S. Gas-chromatographic-mass spectrometric confirmation of endosulfan and endosulfan sulfate in apples and carrots. J. Assoc.off. Anal.Chem.1981,64,1208-1210.
[82]于金凤. 硫丹对抗性棉铃虫某些生物学特性的影响[J].农药科学与管理.1999,20(2):18-19
[83]化学工业部农药情报中心站,国外农药品种手册(三)[M].1981:49-53
[84]Forrester,N.W.Australian Cotton Grower,1989,10(3):62-69
[85]王开运等.硫丹对抗性棉蚜、棉铃虫的防治效果及应用展望[J].农药,1993,32(2):2-4
[86]王金信等. 几类农药防治抗性棉蚜的药效评价[J].农药.1997,36(1):8-9
[87]王成菊.丙锈磷等对棉铃虫不同龄期的作用特点研究[J].农药,1996,35(1):30-33
[88]国家环境保护局.化学农药环境安全评价试验准则[M].1989
[89]莫汉宏,安风春等.农药在农田生态环境中的持久性及残留研究方法[J].农药环境化学行为论文集[M],1994,24-29
[90]王守玉.新疆棉铃虫对溴氰菊酯和硫丹抗性的生化机理研究[J].昆虫学报.2000(增刊):44-50
[91]于金凤.棉蚜对不同类型杀虫剂的抗性发生规律[J].山东农业大学学报.1997,28(3):315-319
[92]丁中.硫丹合成工艺的研究[J].农药.2001,40(6):18-21
[93]郜红建.有机氯农药在南京市郊蔬菜中的生物富集与质量安全[J].环境科学报.2005,25(1):90-93
[94]张百臻.长期使用硫丹可能对人身健康和生态环境造成严重危害. 农药科学与管理.2002,23(5):35
[95]陈惠.印度国家腰果研究中心提议禁用硫丹防治虫害.Tcashew.2000,16(4):2
[96]杨伟华等.棉花产品中农药残留情况初步研究[J].中国棉花,研究简报.2006 , 33 (3):12-15
[97]郭明等. 赛丹在棉花和土壤中的残留动态研究[J].农业环境保护,2001,20(4):243-245
[98]Abel, C. A., and G. L. Snodgrass. 2004. Tarnished plant bug development in Teld corn. Proceedings of the Beltwide Cotton Production and Research Conference, National Cotton Council, January, San Antonio, TX.
[99]Tugwell, P., S.C. Young, Jr., B. A. Dumas, and J. R. Phillips. 1976. Plant bugs in cotton: Importance of infestation time, types of cotton injury, and signiTcance of wild hosts near cotton. University of Arkansas Agricultural Experiment Station, U. of Arkansas Ag. Expr. Sta. Rpt. 227. 24 pp. Received for publication 28 October 2005; accepted 21 June 2006.
[100]Pankey, J. H., B. R. Leonard, J. B. Graves, and E. Burris. 1996. Toxicity of acephate, cypermethrin, and oxamyl to tarnished plant bugs in vial bioassays and cage studies on cotton. Proceedings of the Beltwide Cotton Production Research Conference, National Cotton Council, January, Nashville, TN
[101]张骏.食品中硫丹残留的酶联免疫吸附检测方法的研究. 中国食品科技学会第四届年会论文
[102]韩熹莱主编.农药概论.北京农业大学出版社,1995
[103]Funari E,Donati L,Sandroni D,et al.Pesticide levels in groundwater:values and limitations of monitoring[A]//Vighi M,Funari E,ed.Pesticide risk in groundwater[M].Boca Raton:CPC Pres,1995:3-44
[104]欧晓明等.农药结合残留的研究现状及问题[J].农药,2006,45(10):660-663
[105]曹爱华,徐光军,冯涛等.赛丹(endosulfan)在烟草和土壤中的残留研究[J].中国烟草科学,1999,3:40-44
[106]明九雪,钱传范,申继忠.硫丹(endosulfan)在苹果和土壤中的残留动态研究[J].中国农业大学学报,1998,3(3):95-100
[107]吕萧,聂燕,陈子雷等. EL ISA快速测定小麦中硫丹及硫丹硫酸酯[J].中国粮油学报,2006,21(5):41-45
[108]吕萧,任凤山,王文博等.茶叶中硫丹及硫丹硫酸酯的ELISA 快速测定[J].茶叶科学,2006,26(2):141-146
[109]吴景平,司宗兴.硫丹的高效液相色谱分析[J].农药科学与管理,1995,1:12-13
[110]刘小松,黄大新,卢声宇. 毛细管气相色谱法测定南美白对虾中硫丹的残留量[J].色谱,2007,25(3):392-394
[111]陈化才.赛丹在茶叶中的残留降解动态研究[J].农药,2000,39(3):25-27
[112]谭道朝, 蒋廷福. 农药残留分析样品前处理技术发展概述[J].广西植保,2007,20(2):16-20
[113]陈万义,薛振祥,王能武主编.新农药研究与开发[M].北京,化学工业出版社,1995,115-116
[114]农业部农药检定所.农业部行业标准 NY/T 788-2004《农药残留试验准则》[S].北京:中国标准出版社,2004
[115]张韩杰,闰艳春.农药残留及微生物在农药降解中的应用与展望[J].湖北植保 2004,31-35
[116] Ghadiri H, Rose C.W.Degradation of endosulfan in a clay soil from cotton farms of westrn Queensland. Journal of Environmental management, 2001, 62:155-169.
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