摘要
麦红吸浆虫Sitodiplosis mosellana(Gehin)是小麦生产中间歇性猖獗成灾的毁灭性害虫。在我国20世纪50和80年代曾两次大面积暴发成灾,损失惨重。近年来,麦红吸浆虫在我国大范围猖獗成灾的势头得到了有效的遏制,但局部地区大发生的现象仍时有发生,并具有扩散蔓延的趋势,对小麦生产的威胁并未解除。多年来的研究表明,滞育是引起其猖獗成灾的主要原因之一,但有关麦红吸浆虫的滞育机理,有许多问题至今仍不清楚。本文在前人研究工作的基础上,采用生物化学、蛋白质组学、放射免疫分析和电镜技术等多学科交叉的前沿方法和手段,继续对麦红吸浆虫滞育机理进行深入细致的研究,取得了一定的新进展,为今后进一步深入研究其滞育机理、成灾规律、预测预报、大面积综合治理奠定了良好的基础。主要研究结果如下:
1.应用考马斯亮蓝G-250染色法、SDS-PAGE及PAGE电泳技术分析了麦红吸浆虫滞育前后及滞育期蛋白质含量和组分的变化。结果表明,不同阶段蛋白质含量存在显著差异,老熟幼虫入土前和滞育解除后均高于入土滞育期。入土滞育期间,同期裸露幼虫与结茧幼虫,以及不同滞育年限幼虫的蛋白质含量差异均不显著;滞育年周期中,无论结茧幼虫还是裸露幼虫,蛋白质含量的变化均可划分为两个阶段,第1阶段为当年6月至12月,蛋白质含量较低,平均为19.2269μg/头和19.5734μg/头;第2阶段为翌年2月底至5月中旬,蛋白质含量相对较高,平均为20.9578μg/头和20.7153μg/头。电泳图谱分析发现,SDS-PAGE 18.3kD和32.0kD蛋白带与4条PAGE蛋白带的表达量,以及SDS-PAGE 18.3kD和19.8kD蛋白带的相对表达量在滞育前后及滞育期发生了一定的变化,但在滞育期不同阶段基本无变化。
2.应用蛋白质双向电泳和质谱分析技术对麦红吸浆虫幼虫滞育前、越夏和越冬滞育期及滞育解除后4个阶段蛋白质组的表达进行了研究。结果表明,在考马斯亮蓝染色的双向电泳pH3-10胶上,PDQuest 7.4软件可识别滞育前幼虫蛋白点约300个,其它时期约275个。以滞育前幼虫为参照,越夏、越冬滞育期及滞育解除后幼虫蛋白表达量差异达2倍以上的点分别有91个、92个和95个。对3个在滞育前高表达或特异表达、2个滞育前低表达或缺失、1个冬季滞育期特异表达、2个夏季和冬季滞育期高表达的8个差异显著的斑点进行质谱测定,均获得肽质量指纹图谱,搜索NCBInr数据库初步鉴定出7个蛋白质,它们分别是阿片黑素促皮质素原,NADH脱氢酶亚基1,F10F2.5,IKK相互作用蛋白同型2,GA10647-PA,紫色CG16784-PB同型B和B0228-6,这些蛋白质在麦红吸浆虫滞育过程中发挥着重要作用。
3.用保护酶活性测试盒分别测定了麦红吸浆虫滞育前后及滞育期过氧化物酶(POD)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)等3种保护酶的活力。结果表明,幼虫从老熟到进入滞育的初期,3种保护酶的活力均呈下降趋势。滞育年周期中,SOD和CAT活力对环境温度的反应相同,即低温促进其活力升高,高温导致其活力下降;POD活力与环境温度和滞育发育有关;整个滞育期,裸露幼虫和结茧幼虫3种保护酶的活力随季节变化趋势相同,但同期的裸露幼虫活力略高于结茧幼虫;不同滞育年限幼虫3种保护酶的活力差异不显著。滞育解除后,3种保护酶的活力均随着生长发育进程逐渐升高。
4.用代谢酶活性测试盒分别测定了麦红吸浆虫滞育前后和滞育期糖原磷酸化酶(Gpase)、磷酸果糖激酶(PFK)、己糖激酶(HK)和醛缩酶(ALD)等4种代谢酶活力的变化。结果表明,麦红吸浆虫滞育前后代谢酶活力明显不同,滞育后Gpase活力显著提高,糖酵解有关酶PFK、HK和ALD活力降低,以保证更多的糖原转化为海藻糖;滞育解除后Gpase活力降低,PFK、HK和ALD活力升高,以确保昆虫生长发育所需的物质和能量供给。滞育期间,4种代谢酶的活力均与滞育发育有关;同时Gpase和PFK的活力也与环境温度有关,即夏、冬季高于春、秋季;同期不同滞育状态幼虫比较,裸露幼虫PFK、HK和ALD活力总是略高于结茧幼虫,Gpase则相反。同一时期不同滞育年限幼虫4种代谢酶的活力无显著差异。
5.用放射免疫分析法测定了麦红吸浆虫滞育前后及滞育期全虫体内蜕皮甾类激素滴度的变化。结果表明,入土滞育过程中,同期裸露幼虫与结茧幼虫,以及不同滞育年限幼虫的蜕皮甾类激素滴度差异均不显著。在年发生周期中,滞育前后及滞育期蜕皮甾类激素呈现出明显的动态变化,入土进入滞育后显著降低,入土滞育期间,11月5日以前及二次滞育幼虫较低,且变化幅度较小;冬季寒冷季节蜕皮甾类激素最高,为7.0pg/幼虫左右。滞育解除后蜕皮甾类激素滴度显著降低,之后随着幼虫的生长发育逐渐升高,至化蛹前一周左右达到最高峰,为15.22pg/幼虫。
6.应用电镜技术,对麦红吸浆虫麦穗老熟幼虫和滞育圆茧丝腺细胞进行了超微结构观察。结果发现麦穗老熟幼虫和结茧幼虫丝腺胞质中均分布有发达平行排列的粗面内质网、高尔基体和线粒体。但麦穗老熟幼虫胞质中还分布有发达的环形粗面内质网,高尔基体周围电子致密度高,糖原颗粒含量丰富,腺腔中充满了电子致密度高的丝物质;相反,结茧幼虫高尔基体周围电子致密度低,糖原颗粒含量极微,腺腔丝物质含量低。
The wheat blossom midge,Sitodiplosis mosellana(Gehin),is one of the intermittentlydisastrous insect pests injuring wheat.Two times of broad and disastrous damages wererecorded in China history in 1950's and 1980's respectively.In about ten years recently,thedisaster caused by the wheat blossom midge in a broad area was controlled effectiviely,butthe local damage was still often found in some wheat-growing regions.A trend extending itsdistribution area is obvious.Therefore,the potential threat of the wheat blossom midge to thewheat production did not disappear.It is said that diapause is one of key factors causingrampancy endangerment with intermittent and regional characteristics.However,manyproblems about the diapause mechanisms in wheat blossom midge remain uncertain.In thispaper,diapause mechanism of wheat blossom midge was conducted by means of methods andtechniques of biochemistry,proteomics,electron microscopy and radioimmunoassay on thebasis of previous studies.The present research will provide a foundation for further studies ondiapause regulation mechanism,forecasting,control of the wheat blossom midge.The mainresults were as follows.
1.The comassie brilliant blue dying,SDS-PAGE and PAGE techniques were used toanalyze protein contents and compositions in pre-diapause,diapause and post-diapause larvaeof the wheat blossom midge.The protein content in different diapause stages wassignificantly different,which in pre-diapause and post-diapause larvae were higher than thosein diapause larvae.However,no obvious difference of protein content was observed between1st and 2nd year of diapause larvae,as well as between cocooned and non-cocooned larvae inthe same diapause periods.Based on the protein contents,the larval diapause in the wheatblossom midge larvae could be divided into two stages during diapause cycle:stage 1 is fromJune to December,protein average content 19.2269μg/individual in cocooned larvae and19.5734μg/individual in non-cocooned larvae.The stage 2 from late February to mid-May ofnext year,average 20.9578 and 20.7153μg/individual respectively,both higher than stage 1.According to the electrophoresis diagrams,expressions levels of two protein bands(18.3 and32.0 kD)from SDS-PAGE,and four protein bands from PAGE,as well as relativeexpressions amounts of protein bands 18.3 and 19.8 kD from SDS-PAGE obviously changed at various periods of pre-diapause,diapause and post-diapause,but their expressions weresimilar in different stages of diapause period.
2.The proteomic approach was adopted to investigate proteins extracted from larvae ofwheat blossom midge at defferent developmental stages including pre-diapause,over-summering diapause,over-wintering diapause and post-diapause.On 2-DE gels stained bycoomassie brilliant blue,about 300 protein spots were detected in pre-diapause,and 275protein spots in each of other periods.There were 91,92 and 95 protein spots that showedmore than 2-fold changes in abundance in larvae at over-summering diapause,over-winteringdiapause and post-diapause in comparison with pre-diapause larvae.Eight protein spots,which were with most significant differences in larvae at different diapause periods,wereanalyzed by Matrix-assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF-MS).Among these eight proteins,three were up-regulated or unique duringpre-diapause,two were down-regulated or absent during pre-diapause,one was unique duringover-wintering diapause period,and the other two were up-regulated during over-summeringand over-wintering diapause stages.Seven of the eight protein spots were successfullyidentified from peptide mass fingerprints on the basis of NCBInr database.They wereproopiomelanocortin,NADH dehydrogenase subunit 1,F10F2.5,IKK interacting proteinisoform 2,GA10647-PA,purple CG16784-PB isoform B and B0228.6,respectively.Theseproteins play important roles in wheat blossom midge.
3.The activities of protective enzymes peroxidase(POD),superoxide dismutase(SOD)and catalase(CAT)in pre-diapause,diapause and post-diapause larvae of wheat blossommidge were determined by using the protective enzyme testing kits.The results indicated thatactivities of the three protective enzymes showed a decreasing trend from pre-diapause toearly diapause.In one-year cycle,SOD and CAT activities of diapause larvae had the sameresponses to environment temperature,i.e.,increased at lower temperature but decreased athigher temperature,while POD activity was related to both environmental temperature anddevelopment situation of the larvae.A similar seasonal variation trend was observed in thethree protective enzymes of both no-cocooned and cocooned larvae in one-year cycle.Thethree protective enzyme activities of no-cocooned larvae were higher than those of cocoonedlarvae at the same diapause stage.No significant difference of the three protective enzymeactivities was found between 1st and 2nd year diapause larvae.The activities of the threeprotective enzymes increased gradually with the development of post-diapause larvae.
4.The activities of metabolic enzymes Hexokinase(HK),Aldolase(ALD),Glycogenphosphorylase(Gpase)and Phosphofructokinase(PFK)in pre-diapause,diapause andpost-diapause larvae of wheat blossom midge were measured using testing kits.Flexible metabolic rearrangement was observed in diapause processing of S.mosellana.Gpase activitywas strengthened significantly,and activities of glycolysis enzymes(HK,ALK,PFK)wereweakened significantly after entering diapause,which ensured more carbon flowing intotrehalose synthesis.On the contrary,Gpase activity decreased,and activities of glycolysisenzymes increased after diapause termination,which ensured to form glycogen from trehaloseto supply energy for development.In one-year diapause cycle,activities of four enzymes wereall related with development situation of diapause larvae.Gpase and PFK were alsoinfluenced by environmental temperature.At the same diapause stage,activities of HK,ALKand PFK of no-cocooned larvae were higher than those of the cocooned larvae.On thecontrary,Gpase activities of no-cocooned larvae were lower than those of the cocooned larvae.No obvious difference of four metabolic enzyme activities was found between 1st and 2ndyear diapause larvae.
5.The ecdysteroid titers in pre-diapause,diapause and post-diapause larvae of wheatblossom midge were determined by radioimmunoassay.No obvious difference of ecdysteroidtiters was found between 1 st and 2nd year of diapause larvae,as well as between no-cocoonedand cocooned larvae in the same diapause stages.In one-year cycle,the ecdysteroid titersshowed obviously a dynamic changes,which decreased significantly after diapausing,andthose of over-wintering larvae were highest(about 7.0pg/larva)during diapausing,whilethose of other season were low and had a minor difference.The ecdysteroid titers reducedsignificantly after diapause termination,and increased gradually with the development andreached peak level(15.22 pg/larva)about one week before pupation.
6.By means of electron microscopy,ultrastructure of silk glands in S.mosellana larvaecollected from wheat ear and cocooned larvae from soil were observed.The results showedthat parallelly rough endoplasmic reticulum,golgi apparatus and mitochondrion were all verydeveloped in silk glands cell of larvae both from wheat ear and soil.Moreover,annularlyrough endoplasmic reticulum,which was very complex,was found in silk gland cells oflarvae from wheat ear.Glycogenosome,secretory granules around golgi apparatus,and silkmaterial of glandular cavity were richer in silk glands cell of larvae from wheat ear than thoseof cocooned larvae.
引文
[1]王方海,龚和.滞育激素的研究进展[J].昆虫天敌,1997,19(2):88-92.
[2]Tauber MJ,Tauber CA,Masaki S.Seasonal Adaption of Insects[M].New York:Oxford University Press,1986.
[3]苏天运,苏天增.昆虫滞育生化机制研究概况(下)[J].四川动物,1995,14(4):166-170.
[4]Xue FS,Kallenborn HG.Dispersive breeding in agricultural pest insects and its adaptive significance[J].Journal of Applied Entomology,1993,116:170-177.
[5]Danks HV.The range of insect dormancy responses[J].Eur.J.Entomol.,2002,99:127-142.
[6]Danilevsky AS.Photoperiodism and seasonal development of insect[M].London:Oliver and Boyd,1965.
[7]徐卫华.昆虫滞育的研究进展[J].昆虫学报,1999,42(1):100-107.
[8]Yin CM,Chippendale GM.Insect frontal ganglion:fine structure of its neurosecretory ceils in diapause and non-diapause larvae of Diatraea grandiosella[J].Canadian Journal of Zoology,1975,53:1093-1100.
[9]Yagi S.Fukaya M.Juvenile hormone as a key factor regulating larval diapause of the rice stem borer Chilo suppressalis[J].Applied Entomology and Zoology,1974,9:247-255.
[10]Bean DW,Beck SD.Juvenile hormone esterases in diapause and nondiapause larvae of the European corn borer Ostrinia nubilalis[J].J.Insect Physiol.,1982,28:485-492.
[11]Salama MS,Miller TA.In vitro translation of diapause mRNA from the fat body of active and diapause larvae of the pink bollworm Pectinophora gossypiella(Saunders)[J].Arch.Insect Biochem.Physiol.,1993,23(1):1-11.
[12]Zhang TY,Sun JS,Liu WY,et al.Structural characterization and transcriptional regulation of the gene encoding diapause hormone and pheromone biosynthesis activating neuropeptide in the cotton bollworm,Helicoverpa armigera[J].Biochim.Biophys.Acta.,2005,1728:44-52.
[13]Denlingcr DL,Wingard P.Cyclic GMP breaks pupal diapause in the flesh fly Sarcophaga crassipalpis[J].J.Insect Physiol.,1978,24:715-719.
[14]Koopmanschap AB,Lammersand JHM,De kort CAD.The structure of the gene encoding diapause protein of the colorada potato beetle,Leptinotarsa decemlineata[J].J.Insect Physiol.,1995,41(6):509-518.
[15]Kostal V,Tamura M,Tollarova M,et al.Enzymatic capacity for accumulation of polyol cryoprotectants changes during diapause development in the adult red firebug,Pyrrhocoris apterus[J].Physiological Entomology,2004,29:344-355.
[16]苏天运,王仲文,王梅英.淡色库蚊越冬的生态生理学研究[J].河南医科大学学报,1993,28(2):97-101.
[17]张恩英,高农,王仁资.滞育三带喙库蚊和淡色库蚊的脂肪含量和耐寒比较[J].上海第二军医大学学报,1993,13(2):120-122.
[18]陈天业,牟吉元.草蛉的滞育[J].昆虫知识,1996,33(1):56-58.
[19]Beck SD.Insect Photoperiodism(2nded)[M].NewYork:Academic Press,1980.
[20]Saunders DS.Insect Clock(3rded)[M].New York:Academic Press,2002.
[21]Beck SD.Effect of temperature on thermoperiodic determination of diapause[J].J.Insect Physiol.,1984,30(5):383-386.
[22]肖海军,魏晓棠,黄丽莉,等.昆虫滞育诱导的光周期反应类型[J].江西农业大学学报,2004,26(6):867-873.
[23]Peterson DM,Hamner WM.Photoperiodic control of diapause in the codling moth[J].J.Insect Physiol.,1968,14(4):519-528.
[24]Riedl H,Croft BA.The effects of photoperiod and effective temperatures on the seasonal phenology of the colding moth(Lepidoptera:Tortrickdae).Canada Entomologist,1978,110(5):465-470.
[25]李文香,李建成,路子云,等.中红侧沟茧蜂滞育临界光周期和敏感光照虫态的测定[J].昆虫学报,2008,51(6):635-639
[26]Masaki S.Ecological significance of diapause in the seasonal cycle of Abraxas Miranda Btl.Bull[J].Fac.Agr.Mie.Univ.,1957,15:15-24.
[27]Cullen JM,Browning TO.The influence of photoperiod and temperature on the induction of diapause in pupae of Heliothis punctigera[J].J.Insect Physiol.,1978,25:647-652.
[28]Xue FS,Kallenborn HG.Summer and winter diapause in pupae of the cabbage butterfly,Pieris melete M(?)n(?)tri(?)s[J].J.Insect Physiol.,1997,43:701-707.
[29]Kroon A,Veenendall RL,Veerman A.Photoperiodic induction of diapause in the spider mite Tetranychus urticae:Qualitative or quantitative time measurement[J].Physiol.Entomol.,1997,22:357-364.
[30]Xue FS,Kallenborn HG.Control of summer and winter diapause in Pidorus euchromioides(Lepodoptera:Zygaenidae)on Chinese sweetleaf Symplocos chinensis Bull[J].Entonol.Res.,1998,88:207-211.
[31]赖锡婷,肖海军,薛芳森.昆虫滞育持续时间的影响因子及其对滞育后生物学的影响[J].昆虫知识,2008,45(2):182-188.
[32]Tauber M J,Tauber CA.Thermal accumulations,diapause,and oviposition in a conifer-inhabiting predator,Chrysopa harrisii(Neuroptera)[J].Canada Entomologist,1974,106(9):969-978.
[33]Pener MP,Orshan L.Reversible reproductive diapause and intermediate states between diapause and full reproductive activity in male Oedipoda miniata grasshoppers[J].Physiological Entomology,1980,5(4):417-426.
[34]Obrycki JJ,Tauber MJ,Tauber CA,et al.Environmental control of the seasonal life cycle of Adalia bipunctata(Coleoptera:Coccinellidae)[J].Environmental Entomology,1983,12(2):416-421.
[35]Havelka Y.Some aspects of the photeperiodism of the carnivorous gall midge Aphidoletes aphidimyza(Diptera:Cecidomyiidae)[J].Entomologicheskoe Obozrenie,1980,59(2):241-248.
[36]Barker RJ.Inhibition of diapause in Pieris rapae L.by brief supplementary photophases[J].Experientia,1963,19:185.
[37]吴少会,李峰,周昱晨,等.暗期干扰对环带锦斑蛾滞育诱导的影响[J].昆虫学报,2007,50(7):703-708.
[38]Hua A,Xue FS,Xiao H J,et al.Photoperiodic counter of diapause induction in Pseudopidorus fasciata(Lepidoptera:Zygaenidae)[J].J.Insect Physiol.,2005,51(12):1287-1294.
[39]Hua A,Yang D,Wu S,Xue FS.Photoperiodic control of diapausein Pseudopidorus faseiata (Lepidoptera:Zygaenidae)based on aqualitative time measurement[J].J.Insect Physiol.,2005,51:1261-1267.
[40]王庆忠.不同光照对一化性柞蚕解除滞育的影响[J].蚕学通讯,2008,28(1):19-24.
[41]Meleod DGR,BecK S D.Photoperiodic termination of diapause in an insect[J].Biol.Bull.,1963,124:84-96.
[42]Tauber M J,Tauber CA.Quantitative respinse to daylength during diapause in insect[J].Nature,1973,244:296-297.
[43]Numata H,Hidaka T.Photoperiodic control of adult diapause in the bean bug,Piptortus clavatus Thunberg(Heteroptera:Coreidae).Ⅰ.Reversible induction and termination of diapause[J].Appl.Ent.Zool.,1982,17(4):530-538.
[44]昊孔明,郭予元.棉铃虫滞育的诱导因素研究[J].植物保护学报,1995,22(4):331-336.
[45]Ishikawa Y,Yamashita T,Nomura M.Characteristics of summer diapause in the onion maggot,Delia antiqua(Diptera:Anthomyiidae)[J].J.Insect Physiol.,2000,46:161-167.
[46]Numata H,Shiga S.Induction of adult diapause by photoperiod and temperature in Protophormia terraenovae(Diptera:Calliphoridae)in Japan[J].Environmental Entomology,1995,24(6):1633-1636.
[47]Blanckenhorn WU.Altitudinal differentiation in the diapause response of two species of dung flies[J].Ecol.Entomol.,1998,23:1-8.
[48]Xue FS,Zhu XF,Shao ZY.Control of summer winter diapause in the leaf-mining fly Pegomyia bicolor(Diptera:Antomylidae)[J].J.Appl.Ent.,2001,125:181-187.
[49]Garcia PV,Wajnberg E,Pizzo I J,et al.Diapause in the egg parasitoid Trichogramma cordubensis:role of temperature[J].J.Insect Physiol.,2002,48:349-452.
[50]Fujlyama S,Talahashi F.The survival rate and the duration of development in Anomala cuprea(Coleoptera:Scarabaeidae)under thermal changes along with its developmental stage[J].Environ.Control Biol.,1977,15:19-25.
[51]Baker JE.Termination of larval diapause-like condition in Attagenus megatoma(Coleoptera:Dermestidae)by low temperature[J].Envion.Entomol.,1982,11:506-508.
[52]Houten YMK,Overmeer WPJ,ZonAQ,et al.Thermoperiodic induction of diapause in the predacious mite,Amblyseius potentillae[J].J.Insect Physiol.,1988,34(4):285-290.
[53]Saunders DS.Under-sized larvae from short-day adults of the blow fly Calliphora vicina side-step the diapause programme[J].Physiol.Entomol.,1997,22:249-255.
[54]王小平,薛芳森,华爱,等.食料因子对昆虫滞育及滞育后发育的影响[J].江西农业大学学报,2004,26(1):10-16.
[55]Hare JD.Seasonal variation in plant-insect associations:Utilization of Solanum dulcamara by Leptinotarsa decemlineata[J].Ecology,1983,64:345-361.
[56]Foster DR,Crowder LA.Diapause of the pink bollworm Pectinophora gossypiella related to dietary lipids[J].Comp.Biochem.Physiol.,1980,65B:723-726.
[57]Dingle H.Movement of highly mobile insects:concepts and methodology in research[A].Adaptive variation in the evolution of insect migration[C].Raleigh:North Carolina State University Press,1979:64-87.
[58]Bradshaw WE.Major environmental factors inducing the termination of larval diapause in Chaoborus americanus Johannsen(Diptera:Culicidae)[J].Biol.Bull.,1969,136:2-8.
[59]Bell CH.Factors governing the induction of diapause in Ephestia elutella and Plodia interpunctella[J].Physiol.Entomol.,1976,1:83-91.
[60]Fontes EG,Pires CSS,Sujii ER.Mixed risk-spreading strategies and the population dynamics of a Brazilian pasture pest,Deois flavopicta(Homoptera:Cercopidae)[J].Journal of Economic Entomology,1995,88(5):1256-1262.
[61]牟吉元,徐洪富,李火苟.昆虫生态与农业害虫预测预报[M].北京:中国农业科技出版社,1997.
[62]李超,谢宝瑜.光周期和温度的联合作用对棉铃虫种群滞育的影响[J].昆虫知识,1981,18:58-61.
[63]Kono Y,Kobayashi M,Claret J.A putative photoreceptoroganelle in insect brain Glial cell[J].Appl.Entomol.Zool.,1983,1:116-121.
[64]Taleda M.Photoperiodic time measurement and related physiological mechanisms in insects and mites[J].Ann.Rev.Entomol.,1997,42:323-349.
[65]徐卫华,张元琪,王盈,等.昆虫滞育的分子机理—家蚕胚子发育期的温度条件与滞育基因表达[J].中国科学(C辑),1998,28(2):154-159.
[66]Usua EJ.Diapause in the maize stem borer[J].J.Econ.Ent.,1970,63:1605-1610.
[67]Scheltes P.The condition of the host plant during aestivation diapause of the stalk borers Chilo partellus and Chilo orichallcociliella(Lepidoptera:Pyralidae)in Kenya[J].Ent.Exp.App.,1978,24:679-688.
[68]Chippendale GM.Larval and pupal diapause[A].Endocrinology of insects[C].New York:Alan R Liss Inc Press,1983:343-356.
[69]Honek A.Relation of colour changes and diapause on the phenology of Chrysopa cornea Steph[J].Acta Entomol.Bohemoslov.,1973,70:368-375.
[70]许永玉.中华通草蛉的滞育机制和应用[D].浙江大学博士论文,2001.
[71]蒋明星,张孝羲.南京地区棉铃虫越冬蛹滞育的解除与发育[J].昆虫学报,1997,40(4):366-373.
[72]杨秋生,王小平,徐淑,等.丝带凤蝶滞育与非滞育蛹及其成虫的形态学观察[J].昆虫学报,2008,51(4):454-458.
[73]Kotaki T,Yagi S.Hormonal control of adult diapause in the brown-winged green bug,Plautia stali Scott(Heteroptera:Pentatomidae)[J].Appl.Ent.Zool.,1989,24(1):42-51.
[74]De Kort CAD.Thirty five years of diapause research with the Colorado potato beetle[J].Entomol.Exp.Appl.,1990,56:1-13
[75]王方海,龚和,钦俊德.滞育和非滞育棉铃虫血淋巴中蛋白质含量及图谱的比较[J].昆虫学报,1998,41(4):426-430
[76]Salama MS,Miller TA.A diapause associated protein of the pink bollworm Pectinophora gossypiella Saunders[J].Arch.Insect Biochem.Physiol.,1992,21(1):1-11.
[77]关雪辰,陈娥英.七星飘虫成虫脂肪体核酸代谢与生殖滞育的关系[J].昆虫学报,1982,25(3):239-243.
[78]赵章武,黄永平.昆虫滞育关联蛋白的研究进展[J].昆虫知识,1996,33(3):187-191.
[79]De Loof A,De wilde J.Hormonal control of synthesis of vitellogenic female protein in the Colorado beetle,Leptinotarsa decemliineata[J].J.Insect Physiol.,1970,16:1455-1466.
[80]Brown J J,Chippendale GM.Juvenile hormone and a protein associated with the larval diapause of the southwestern corn borer,Diatraea grandiosella[J].Insect Biochemistry,1978,8:359-367.
[81]Brown JJ.Haemolymph protein reserves of diapausing codling moth larvae,Cydia pomonella(Lepidoptera:Tortricidae)[J].Arch.Insect Biochem.Physiol.,1980,26:487-491.
[82]Ichimori T,Ohtomo R,Suzuki K,et al.Specific protein related to adult diapause in the leaf beetle,Gasteophysa atrocyanea[J].J.Insect Physiol.,1990,36:85-91.
[83]Osir EO,Labongo LV,Unntithan GC.A high molecular weight diapause associated protein from the stem borer,Busseola fusca:purification and properties[J].Arch.Insect Biochem.Physiol.,1989,11:173-187.
[84]Koopmanschap AB,Lammers H,De Kort S.Storage proteins are present in the hemolymph from larvae and adults of the Colorado potato beetle[J].Arch.Insect Biochem.Physiol.,1992,20(2):119-133.
[85]毛文富,曹梅讯.亚洲玉米螟滞育关联蛋白的分离和纯化[J].昆虫学报,2001,11(11):389-394.
[86]Lewis DK,Spurgeon D,Sappington TW,et al.A hexamerin protein,AgSP-1,is associated with diapause in the boll weevil[J].J.Insect Physiol.,2002,48:887-901.
[87]Sonobe H,Odake H.Studies on embryonic diapause in the pnd mutant of the silkworm,Bombyx mori[J].Roux's Arch.Dev.Biol.,1986,195:229-235.
[88]Lee KY,Denlinger DL.Diapause-regulated Proteins in the Gut of Pharate First Instar Larvae of the Gypsy Moth,Lymantria dispar,and the Effect of KK-42 and Neck Ligation on Expression[J].J.Insect Physiol.,1996,42(5):423-431.
[89]Joplin KH,Yocum GD,Denlinger DL.Diapause specific protein expressed by the brain during the diapause of the flesh fly,Sarcophaga crassipalpis[J].J.Insect Physiol.,1990,36:775-783.
[90]Hayward SAL,Pavlides SC,Tammariello SP,et al.Temporal expression patterns of diapause associated genes in flesh fly pupae from the onset of diapause through post-diapause quiescence[J].J.Insect Physiol.,2005,51:631-640.
[91]Li AQ,Butler AP,Dean DH,et al.Proteomics of the flesh fly brain reveals an abundance of upregulated heat shock proteins during pupal diapause[J].J.Insect Physiol.,2007,53:385-391.
[92]De Kort CAD,Koopmanschap AB,Vermunt AMV.Influence of pyriproxyfen on the expression of haemolymph protein genes in the Colorado potato beetle,Leptinotarsa decemlineata[J].J.Insect Physiol.,1997,43:363-371.
[93]De Kort CAD,KooPmansehap AB.Nucleotide and deduced amino acid sequenee of a cDNA clone encoding diapause proteinI an aryi-phorin-type storage hexamer of the Colorado potato beetle[J].J.Insect Physiol.,1994,40(6):527-535.
[94]Palli SR,Ladd TR,Ricci AR,et al.Synthesis of the same two proteins prior to larvael diapause and pupation in the spruce budworm Choristoneura fumiferana[J].J.Insect Physiol.,1998,44:509-524.
[95]Godlewski J,Kludkiewicz B,Grzelak K,et al.Expression of larval hemolymph proteins(Lhp)genes and protein synthesis in the fat body of greater wax moth(Galleria mellonella)larvae during diapause[J].J.Insect Physiol.,2001,47:759-766.
[96]Yocum GD.Isolation and characterization of three diapause associated transcripts from the Colorado potato beetle,Leptinotarsa decemliineata[J].J.Insect Physiol.,2003,49:161-169.
[97]赵亮,刘莉,王爽,等.采用蛋白质组学技术筛选大肠癌转移相关蛋白[J].生物化学与生物物理进展,2006,33(5):485-491.
[98]Furusawa T,Shikata M,Yamashit O.Temperature dependent sorbitol utilization in diapause eggs of the silk worm,Bombyx mori[J].J.Comp.Physiol B.,1982,147:21-26.
[99]Hasegawa K,Yamashita O.Studies on the mode of action of the diapause hormone in the silkworm Bombyx mori.Vl.The target organ of the diapause hormone[J].J.Exp.Biol.,1965,43:271-277.
[100]Ikeda M,Su ZH,Sato Y,et al.Induction of embryonic diapause and stimulation of overy trehalase activity in the silkworm Bombyx mori by synthetic diapause hormone[J].J.Insect Physiol.,1993,39:889-895.
[101]Yamashita O,Hasegawa K.Studies on the mode of action of diapause hormone in the silkworm Bombyx mori L.Ⅳ.Effect of diapause hormone on the glycogen on content in ovaries and the blood sugar level of silkworms[J].J.Seric.Sci.,1964,33:407-416.
[102]Yamashita O,Hasegawa K.Diapause hormone action in silkworms ovaries incubated in vitro.~(14)C-trehalose incorporation into glycogen[J].J.Insect Physiol.,1976,22:409-414.
[103]Niimi T,Yaginuma T.Biosynthesis of NAD-sorbitol dehydrogenase is induced by accumulation at 5℃ in diapause eggs of the silkworm,Bornbyx mori[J].Comp.Biochem.Physiol.,1992,102 B(1):169-173.
[104]Yaginuma T,Yamashita O.NAD-dependent sorbitol dehydrogenase activity in relation to the termination of diapause in eggs of Bombyx mori[J].Insect Biochem.,1979,9:547-553.
[105]吴大洋,向仲怀,古泽寿治.合成滞育激素对家蚕卵GpaseNAD-SDH、PFK、PBF活性的影响[J].蚕业科学,2002,22(1):1-5.
[106]Li YP,Ding L,Goto M.Seasonal changes in glycerol content and enzyme activities in overwintering larvae of the Shonai ecotype of the rice stem borer,Chilo suppressalis Walker[J].Arch.Insect Biochem.Phvsiol.,2002,50(2):53-61.
[107]王满囷,李周直.鞭角华扁叶蜂预蛹脂肪体和血淋巴中糖类含量动态[J].南京林业大学学报,2002,26(2):21-23.
[108]陆明贤.柞蚕蛹脂肪体和血淋巴中糖类含量动态的温度和光周期效应[J].昆虫学报,1992,35(1):1-7.
[109]Singtripop T,Tungjitwitayakui J,Sakurai S.Intensity of larval diapause in the bamboo borer,Omphisa fuscidentalis[J].Zoolog.Sci.,2002,19:577-582.
[110]朱芬,李红,王永,等.大斑芫菁滞育幼虫在滞育不同阶段体内糖类和醇类含量的变化[J].昆虫学报,2008,51(1):9-13.
[111]张韵梅.棉铃虫蛹在滞育中脂肪、糖元等生化成份含量变化的研究[J].山东农业大学学报,1994,25(2):147-150.
[112]Wipking W,Viebahn M,Neumann D.Oxygen consumption,water,lipid and glycogen content of early and late diapause and non-diapause larvae of the burnet moth Zygaena trtfolii[J].J.Insect Physiol.,1995,41(1):47-56.
[113]吴大洋.家蚕滞育的生理学研究[D].西南农业大学博士论文,2002.
[114]苏天运.淡色库蚊越冬过程中体重及脂、蛋白质、碳水化合物含量的动态变化[J].中国媒介生物学及控制杂志,1992,3(6):337-341.
[115]Brown JJ,Chippendale GM.Ultrastructure and respiration of the fat body of diapausing and nondiapausing larvae of the corn borer,Diatraea grandiosalla[J].J.Insect Physiol.,1977,15:395-402.
[116]Furusawa T,Nishida M,Narutaki A.Changes in fatty acid composition of phosphoglycerides during the diapause and embryonic development of Japanese oak silkworm,Antheraea yamamai[J].Journal of Sericultural Science of Japan,1994,63(1):57-63.
[117]Buffington JD,Zar JH.Changes in fatty acid composition of Culex pipiens pipiens during hibernation[J].Ann.Entomol.Soc.Am.,1968,61(3):774-775.
[118]Schaefer CH,Washino RK.Changes in the composition of lipids and fatty acids in adult Culex tarsalis and Anopheles freeborni during the overwintering period[J].J.Insect Physiol.,1969,15(3):395-402.
[119]薛瑞德,周方,王令春,等.三带喙库蚊生殖滞育与其体内脂肪酸变化的关系[J].昆虫学报,1991,34(3):297-302.
[120]王智渝,胡敦孝,王宗舜.棉铃虫滞育蛹和非滞育蛹血淋巴某些生化特性的比较研[J].中国农业学学,1998,3(1):33-39.
[121]Morgan TD,Chippendale GM.Free amino acidsof the haemolymph of the southwest-ern corn borer in relation to their diapause[J].J.Insect Physiol.,1983,24:735-743.
[122]Goto M,Fuji M,Suzuki K,et al.Factors affecting carbohydrate and free amino acid content in overwintering larvae ofEnosima leucotaeniella[J].J.Insect Physiol.,1998,44:87-94.
[123]Hanzal R,Jegorov A.Changes in free amino acid composition in haemolymph of larvae of the wax moth,Galleria mellonella L.during cold acclimation[J].Comp.Biochem.Physiol.,1991,100 A:957-962.
[124]Yang J,Shimizu Y,Suzuki K.A difference in free amino acids in the pupal brains between diapause and nondiapause of the silkworm Bombyx mori[J].Journal of Sericultural Science of Japan,1992,61(1):28-31.
[125]刘婷,吴伟坚.滞育和滞育后越北腹露蝗卵中游离氨基酸的变化[J].华南农业大学学报,2008,29(1):35-38.
[126]Ring RA.Changes in dry weight,protein and nucleic acid contents during diapause and normal development of the blowtly,Lucilia sericata[J].J.Insect Physiol.,1973,19(3):481-494.
[127]Price GM.Nucleic acids in the larva of the blowfly Calliphora erythrocephala[J].J.Insect Physiol.,1965,11:869-878.
[128]Kubota I,Isobe M,Imai K,et al.Characterization of the silkworm diapause hormone[J].Agric.Biol.Chem.,1979,43:1075-1078.
[129]薛瑞德,陆宝麟.光周期对三带库蚊发育期间体内核酸动态的影响[J].昆虫学报,1991,34(2):173-177.
[130]Song HS,An Y,Suzuki K.Dynamic changes of free nucleotides in the silkworm undergoing embryonic diapause induced by environmental factors[J].Journal of Molecular Cell Biology,2007,40(2):91-97.
[131]涂岩等,张良慧.陆生昆虫呼吸代谢的简便测定方法[J].昆虫知识,2002,40(5):474-475.
[132]吴坤君,龚佩瑜,李秀珍.棉铃虫越冬蛹呼吸代谢的某些特点[J].昆虫学报,1989,32(2):136-143.
[133]Phillips JR,Newsom LD.Diapause in Heliothis zea and Heliothis viresscens(Lepidoptera:Noc-tuidae)[J].Ann.Ent.Soc.Am.,1966,59:154-159.
[134]南京农学院主编.昆虫生态及预测预报[M].北京:农业出版社,1985.
[135]Denlingcr DL,Willis JH,Fraenkel G.Rates and cycles of oxygen consumption during pupal diapause in Sarcophaga flesh flies[J].J.Insectphysiol.,1972,18:871-882.
[136]王满囷,庞辉,李周直.鞭角华扁叶蜂预蛹呼吸代谢的特点[J].林业科学研究,2001,14(6):616-620.
[137]Goto M,Sekine Y,Outa H,et al.Relationships between cold hardiness and diapause,and between glycerol and free amino acid contents in overwintering larvae of the oriental corn borer,Ostrinia furnacalis[J].J.Insect Physiol.,2001,47:157-165.
[138]Trofimov VB.Some unusual physiological characteristics of diapause in Neodiprion sertifer[J].Ekologiya,1975,5:85-88.
[139]薛芳森,李保同,朱杏芬.黑纹粉蝶滞育蛹体内超氧化物酶活力的初步研究[J].江西植保,1996,19(1):34-35.
[140]薛芳森,魏洪义,朱杏芬.黑纹粉蝶滞育蛹体内过氧化氢酶活力的研究[J].植物保护学报,1997,24(3):204-207.
[14l]王满囷,李周直.滞育期间鞭角华扁叶蜂保护酶系统活力[J].林业科学,2002,38(4):100-104.
[142]林炜,刘玉娣,侯茂林,等.不同地理种群二化螟滞育和解除滞育幼虫的抗逆性酶活性比较[J].植物保护,2007,33(5):84-87.
[143]肖海军,熊件妹,薛芳森.二化螟滞育幼虫与非滞育幼虫的抗药性比较研究[J].现代生物医学研究,2006,6(12):51-52.
[144]Roller H,Dahm KH,Sweeley CC,et al.The Structureof Juvenile homone[J].Angew Chem.,1967,6:179-180.
[145]Visscher SN.The embryonic diapause of Aulocara elliott(Orthoptera:Acrididae):histological and morphometric changes during diapause development and following experimental termination with juvenile hormone analogue[J].Cell and Tissue Research,1976,174:433-452.
[146]李晚忱,付凤玲,吴玉澄.家蚕滞育性卵胚胎发育过程中蜕皮激素和保幼激素含量的变化[J].蚕业科学,1999,25(2):97-101.
[147]Yin CM,Chippendale GM.Ultrastructural characteristics of insect corpora allata in relation to larval diapause[J].Cell and Tissue Research,1979,197:453-461.
[148]Chippendale GM.Hormonal regulation of larval diapause[J].Annu Rev Entomol.,1977,22:121-138.
[149]Yagi S.Physiological aspects of diapause in rice stem borers and the effect of juvenile hormone[J].Entomol.Gen.,1981,7:213-221.
[150]Yin CM,Chippendale GM.Juvenile hormone regulation of the larval diapause of the southwestern comborer Diatraea grandiosella[J].J.Insect Physiol.,1973,19:2403-2420.
[151]Yin CM,Chippendale GM.Juvenile hormone and the induction of larval polymorphism and diapause of the southwestern corn borer Diatraea grandiosella[J].J.Insect Physiol.,1974,20:1833-1847.
[152]Yin CM,Chippendale GM.Hormonal control of larval diapause and metamorphosis of the southwestern corn borer,Diatraea gradiosella[J].J.Exp.Biol.,1976,64:303-310.
[153]Yin CM,Chippendale GM.Diapause of the southwestern corn bore Diatraea gradiosella:Further evidence showing Juvenile homone to be the regulation[J].J.Insect Physiol.,1979,25:513-523.
[154]Takeda N.Histophysiological studies on corpus allatum during prepupal diapause in Monema flavescens(Lepidoptera)[J].J.Morph.,1977,153:245-262.
[155]Chippendale GM,Yin CM.Larval diapause of the European corn barer Ostrinia nubilalis:further experiments examining its hormonal control[J].J.Insect Physiol.,1979,25:53-58.
[156]Sieber R,Benz G.Juvenile hormone in larval diapause of the codling moth Laspeyresia pomonella (Lepidoptera:Tortricidae)[J].Experientia,1977,33:1598-1599.
[157]Sieber R,Benz G.The hormonal regulation of the larval diapause in the codling moth Laspeyresia pomonella(Lepidoptera:Tortricidae).J.Insect Physiol.,1980,26:213-218.
[158]Highnam KC.Activity of the brain/corpora cardiaca system during pupal diapause brcak in Mimas tiliae(Lepidoptera)[J].Quart.J.Mic.Sci.,1958,99:73-88.
[159]Highnam KC.Activity of the corpora allata during pupal diapause in in Mimas tiliae(Lepidoptera)[J].Quart.J.Mic.Sci.,1958,99:171-180.
[160]Williams CM.Physiology of insect diapause.Ⅳ:The brain and Prothoracic glands as an endocrine system in the cecropia silkworm[J].Biol.Bull.,1952,103:120-138.
[161]Williams CM.The Juvenlie hormone.Ⅱ:Its role in the endocrine control of molting pupation and adult development in the cecropia silkworm[J].Biol.Bull.,1961,121:1582-1585.
[162]Ichikawa M,Nishii T.Studies on the role of the corpus allatum in the silkworm Philosarnia cynthia ricini[J].Biol.Bull.,1959,116:88-94.
[163]Walker GP,Denlinger DL.Juvenile hormone and moulting hormone titres in diapause and nondiapause-destined flesh flies[J].J.Insect Physiol.,1980,26:661-664.
[164]Denlinger DL.The physiology of pupal diapause in flesh flies[A].Current Topics in insect Endocrinology and Nutrition[C].New York:Plenum Press,1981:131-160.
[165]Denlinger DL.Hormonal and metabolic aspects of pupal diapause in Diptera[J].Ent.Gen.,1981,7:245-259.
[166]Denlinger DL,Shukla M,Faustini DL.Juvenile hormone involvement in pupal diapause of the flesh fly Sorcopiraga crassipalpis:regulation of infradian cycles of O_2 consumption[J].J.Exp.Btol.,1984,109:191-199.
[167]Truman JW,Riddiford LM,Safranek L.Safranek Hormonal control of cuticle coloration in the tobacco hornworm Manduca sexta:basis of an ultrasensitive bioassay for juvenile hormone[J].J.Insect Physiol.,1973,19:195-203.
[168]Safranek L,Riddiford L.The biology of the black larval mutant of the tobacco hornwonn Manduca sexta[J].J.Insect Physiol.,1975,21:1931-1938.
[169]王满困,李周直.鞭角华扁叶蜂保幼激素滴度的动态[J].林业科学,2002,38(5):83-86.
[170]王满困,李周直.鞭角华扁叶蜂预蛹神经内分泌系统的解剖[J].南京林业大学学报,2000,24(2):21-24.
[171]Denlinger DL.Pupal diapause in tropical flesh flies:environmental and endocrine regulation metabolic rate and genetic selection[J].Biol.Bull.,1979,156:31-46.
[172]Bradfleld JY and Denlinger DL.Diapaused evelopment in the tobacco hornworm:A role for ecdysone or juvenile hormone[J].Gen.Comp.Endocr,1980,41:101-107.
[173]Schooneveld H,Sanchez AO,de Wilde J.Juvenile hormone-induced break and termination of diapanse in the Colorado potato beetle[J].J.Insect Physiol.,1977,23:689-696.
[174]De Wilde J.Endocrine aspects of diapause in the adult stage[A].In Endocrinology of Insects[C]New York:Alan R Liss Inc Press,1983:357-367.
[175]Kono Y.Endocrine activities and photopcriodic sensitivity during prediapause period in the phytophagous lady beetle Epilachna vtgintioctopunctata[J].Appl.Ent.Zool.,1980,15:73-80.
[176]Geldilay S.Hormonal control of adult reproductive diapause in the Egyptian grasshopper Anacridium aegyptium L.[J].J.Endocr.,1967,37:63-71.
[177]Girardie A,Granier S.Systeme endocrine of physiologie de la diapause imaginale chezle criquet Egyptien Anacridium aegyptium[J].J.Insect Physiol.,1973,19:2341-2358.
[178]Hodkova M.Function of the neuroendocrine complex in diapausing Pyrrhocoris apterus females[J].J.Insect Physiol.,1977,23:23-28.
[179]Darjo A.Activite des corpora allata et controle Photoperiodique de la maturation ovarienne chez Locusta migratoria[J].J.Insect Physiol.,1976,22:347-355.
[180]Girardie A,Girardie J.Intervention des cellules neurosecretrices medianes dans la castration.parasitaired,Anacridium aegyptium(Orthoptere)[J].J.Insect Physiol.,1977,23:461-467.
[181]Herman WS.Studies on the adult reproductive diapause of themonarch butterfly,Danaus plexippus[J].Biol.Bull.,1981,160:89-106.
[182]苏天运,王仲文,王梅英,等.淡色库蚊越冬过程中体内保幼激素和蜕皮激素的变化[J].河南医学研究,1994,3(2):110-112.
[183]De Wilde J,de Boer JA.Physiology of diapause in the adult Colorado beetle.Ⅱ.Diapause as a case of pseudoallateetomy[J].J.Insect Physiol.,1961,6:152-161.
[184]Poras M.Activite des corps allates de femelles en diapause chez Tetrix undulata(Swrb.)(Orthoptere:Tetrigidae)[J].CR.Acad.Sci.D.,1977,284:1301-1304.
[185]Hodkova M.Nervous pathways in photoperiodic regulation of reproduction infemales of Pyrrhocoris apterus(Hemiptera)[J].Acta Ent.Biochem.,1977,74:353-361.
[186]Hodkova M.Hormonal and nervous inhibition of reproduction by brainin diapausing females of Pyrrhocoris apterus(Hemiptera)[J].Zool.J.Physiol.,1979,83:126-136.
[187]Lewis DK,Spurgeon D,Sappington TW,et al.A hexamerin protein,AgSP-1,is associated with diapause in the boll weevil[J].J.Insect Physiol,2002,48(9):887-901.
[188]Taubmontemayor TE,Palmer JO,Rankin MA.Endocrine regulation of reproduction and diapause in the boll weevil Anthonomus grandis Boheman[J].Arch.Insect Biochem.Physiol.,1997,35(4):455-477.
[189]Shiga S,HamanakaY,Tatsu Y,et al.Juvenile hormone biosyn-thesis in diapause and nondiapause females of the adult blowfly Protophormia terraenovae[J].Zoolog.Sci.,2003,20(10):1199-1206.
[190]Sakurai H,Hirano T,Takeda S.Physiological distinction between aestivation and hibernation in the lady beetle,Coccinella septempunctata bruckii(Coleoptera:Coccinellidae)[J].Appl.Entomol.Zool.,1987,22:286-291.
[191]Lyoussoufi A,Gadenne C,Rieux P,et al.Effects of an insect growth regulator,fenoxycarb,on the diapause of the pear psylla,Cacopsyllapyri[J].Entomol.Exp.Appl.,1994,72(3):239-244.
[192]Ishihara M,Shimada M.Causes of reproductive inactivity and recovery of reproductive abiIity after diapause in females of a multivoltine bruchid Kytorhinus sharpiamus(Coleoptera:Bruchidae)[J].Ann.Entomol.Soc.Am.,1994,87(5):532-537.
[193]Bowers WS,Blickenstaff CC.Hormonal termination of diapause in the alfalfa weevil[J].Science,1966,154:1673-1674.
[194]Kamm J,Swenson KG.Termination of diapause in Draeculacephalus crassicornis with synthetic juvenile hormone[J].J.Econ.Ent.,1972,65:364-367.
[195]Sehnal F.Action of juvenoids on different groups of insect[A].The Juvenile Hormones[C].New York:Plenmn press,1976:301-322.
[196]Ascerno M,Smilowitz EZ,Jr Hower AA.Effects of the insect growth regulator hydroprene on diapausing alfalfa Weevils[J].Environ.Ent.,1981,10:501-505.
[197]Ferenz HJ.Photoperiodic and hormonal control of reproduction in male beetles pterostichus nigrita[J].J.Insect Physiol.,1975,21:331-341.
[198]Saunders SD,Richard DS,Applebaum SW.Photoperiodic diapause in Drosophila melanogaster involves a block to the juvenile hormone regulation of ovarian maturation[J].Gen.Comp Endocrinol.,1990,79(2):174-184.
[199]Butenandt A and Karlson P.Uber die isolierung eines Metamorphose-Hormone der Insekten in Kristallierten Form[J].Z.Naturforsch,1954,96:389-391.
[200]Rees HH.Zooecdysteroids:Structure and occurrence[A].Ecdysone:From Chemistry to Mode of Action[C].Stuttgart:Georg Thieme Verlag Press,1989:28-38.
[201]Lafont R,Horn DHS.Phytoecdysteroids:Structure and occurrence[A].Ecdysone:From Chemistry to Mode of Action[C].Stuttgart:Georg Thieme Verlag Press,1989:39-64.
[202]王方海,龚和.滞育和非滞育棉铃虫血淋巴类固蜕皮素含量变化的比较[J].昆虫学报,1997,40(3):261-264.
[203]Haegele BF,Wang F.Effects of crowding,isolation,and transfer from isolation to crowding on total ecdysteroid content of eggs in Schistocerca gregaria[J].J.Insect Physiol.,2004,50(7):621-628.
[204]Tawfik AI,Vedrova A,Sehnal F.Ecdysteroids during ovarian development and embryo genesis in solitary and Gregarious Schistocerca gregaria[J].Arch.Insect Biochem.Physiol.,1999,41:134-143.
[205]周维善,庄治平.甾体化学进展[M].北京:科学出版社,2002.
[206]Gharib B,Girardie A,De Reggi M.Ecdysteroids and control of embryonic diapause:changes in ecdysteroid levels and exogenous hormone effects in the eggs of cochineal Lepidosaphes[J].Experimentia,1981,37:1107-1108.
[207]Gharib B,Legay JM,De Reggi M.Potentiation of developmental abilities of diapausing eggs of Bombyx mori by 20-hydroxyecdysone[J].J.Insect Physiol.,1981,27:711-713.
[208]Makka T,Seino A,Tomita S,et al.A possible role of 20-hydroxyecdysone in embryonic development of the silkworm Bombyx mori[J].Arch.Insect Biochem.Physiol.,2002,51:111-120.
[209]Ohnishi E,Ohtaki T,Fukuda S.Ecdysone in the eggs of Bombyx silkworm[J].Proceedings of the Japan Academy,1971,47:413-415.
[210]Tawfik AI,Tanaka Y,Tanaka S.Possible involvement of ecdysteroids in embryonic diapause of Locusta migratoria[J].J.Insect Physiol.,2002,48:743-749.
[211]Kidokoro K,Iwata K,Fujiwara Y,et al.Effects of juvenile hormone analogs and 20-hydroxyecdysone on diapause termination in eggs of Locusta migratoria and Oxya yezoensis[J].J.Insect Physiol.,2006(52):473-479.
[212]Church NS.Hormones and the termination and reinduction of diapause in Cephus cinctus Nort.(Hymenoptera:Cephidae)[J].Can.J.Zool.,1955,33:339-369.
[213]Yin CM,GM Chippendale.Insect prothoracic glands:function and ultrastructure in diapause and non-diapause larvae of Diatraea grandiosella[J].Canad.J.Zool.,1975,53:124-131.
[214]Takeda N.Activity mechanisms of the prothoracic glands of Monema Flavescens(Lepidoptera)with special reference to the secretion ofecdysone[J].Biol.Bull.,1976,150:300-321.
[215]Gassier P,Cymborowski B.Physiology of larval diapause in the wax moth,Gallria mellonella:An ultrastructural analysis[J].Comp.Biochem.Physiol.,1993,105A(4):679-689.
[216]甘雅玲,郭中伟.油松毛虫滞育和非滞育期前胸腺超微结构研究[J].电子显微学报,2004,23(4):358.
[217]Ishmail S,Porcheron P,Fuzeau-Braesch S.Variations des ecdysteroids hemolymph-atiques determinees par radioimmunologie et diapause chez Gryllus campestris(Orthopteres)[J].CR.Acad.Sci.D.,1979,289:121-124.
[218]Denlinger DL.Hormonal control of diapause[J].Comprehensive Insect Physioloy Biochemistry and Pharmacolagv,1985,8:353-412.
[219]Walker GP and Denlinger DL.Juvenile hormone and moulting hormone titres in diapause and nondiapause-destined flesh flies[J].J.Insect Physiol.,1980,26:661-664.
[220]Ohtaki T and Takahashi M.Induction and termination of pupal diapause in relation to the change of ecdysone titer in the fleshfly Sarcophagaperegrma Jap[J].J.Med.Sci.Biol.,1972,25:369-376.
[221]Moribayashi A,Kurahashi H,Ohtaki T.Different profiles of ecdysone secretion and its metabolism between diapause and nondiapause destined cultures of the fleshfly,Boettcherisca peregrine[J].Comp.Biochem.Physiol.,1988,91A(1):157-164.
[222]Loeb M.Diapause and development in the tobacco budworm Heliothis viresscens:a comparison of haemolymph ecdysteroid titres[J].J.Insect Physiol.,1982,28:667-673.
[223]Agui N.Activation of prothoracic glands by brains in vitro[J].J.Insect Physiol.,1975,21:903-913.
[224]Herman WS.The ecdysial glands of arthropods[J].Int.Rev.Cytol.,1967,22:269-34.
[225]Joplin KH,Stetson DL,Diaz JG.Cellular differences in ring glands of flesh fly pupae as a consequence ofdiapause programming[J].Tissue Cell,1993,25(2):245-257.
[226]王方海,龚和钦,俊德,等.滞育和非滞育棉铃虫前胸腺的形态解剖学比较研究[J].昆虫学报,1999,42(1):44-47.
[227]Meola RW,Adkisson PL.Release of prothoracicotropic hormone and potentiation of developmental ability during diapause in the bollworm,Heliothis zea[J].J.Insect Physiol.,1977,23:653-655.
[228]Browning TO.Ecdysteroids and diapause in pupae of Heliothis punctigera[J].J.Insect Physiol.,1981,27:715-719.
[229]Williams CM.Ecdysone and ecdysone-analogues:their assay and action on diapausing pupae of the Cynthia silkworm[J].Biol.Bull.,1968,134:344-355.
[230]Zdarek J and Denlinger DL.Action of ecdysteroids,Juvenoids and non-hormonal agents on termination of pupal diapause in the fleshfly[J].J.Insect Physiol.,1975,21:1193-1202.
[231]Gibbs D.The initiation of adult development in Sarcophaga argyrostoma by β-ecdysone[J].J.Insect Physiol.,1976,22:1195-1200.
[232]Tawfik AI,Vedrova A,Li WW,et al.Haemolymph ecdysteroids and the prothoracic glands in the solitary and Gregarious adults of Schistocerca Gregaria[J].J.Insect Physiol.,1996,43(5):485-493.
[233]王方海,张古忍,温瑞贞,等.入侵害虫蔗扁蛾成虫及卵内蜕皮激素的定性定量分析[J].中国科学(C辑),2008,3(1):23-27.
[234]Bownes M,Shirras A,Blair M,et al.Evidence that insect embryogenesis is regulated by ecdysteroids released from yolk proteins[J].Proc.Natl.Acad.Sci.USA.,1988,85(5):1554-1557.
[235]Brier T.Ecdysteroids and adult diapause in the Colorado potato beetle[J].Physiol.Ent.,1982,7: 379-386.
[236]Lefevere KS.Endocrine control of diapause termination in adult female Colorado potato beetle,Leptinotarsa decemlineata[J].J.Insect Physiol.,1989,35(3):197-203.
[237]Matsuo J,Numata H,Tanaka Y,et al.Identification of ecdysteroids and their titer in the hamolymp during development in the bean bug,Riptortus clavatus(Thunberg)(Heteroptera:Alydidae)[J].Appl.Entomol.Zool.,1994,30(1):254-257.
[238]Kono Y.Daily changes of neurosecretory type-Il cell structure of Pieris Larvae entrained by short and long days[J].J.Insect Physiol.,1975,21:249-264.
[239]Kind TV.A study of the reactivation of diapausing pupae of Acronycta rarnicis L.(Leprdoptera Noctuidae).Ⅰ.Cold activation of the prothoractc ghinds in brautless pupae[J].Ent.Rev.,1978,56:13-16.
[240]Kind TV.A study of the reactivation of diapausing pupae of Acrnnycta rurnicis L.(Lepidoptera Noctuidae).Ⅱ.Reacttvation of intact and brainless pupae at various temperatures[J].Ent.Rev.,1978,57:163-167.
[241]Church NS.Hormones and the termination and reinduction of diapause in Cephus cinctus Nort.(Hymenoptera:Cephidae)[J].Can.J.Zool.,1955,33:339-369.
[242]Mitsuhashi J.Histological studies on the neurosecretory cells of the brain and on the corpus allatum during diapause in some lepidopterous insects[J].Bull.Natl.Inst.Agric.Sci.Jap.,1963,16C:67-121.
[243]Raina AK and Bell RA.Morphology of the neuroendocrine system of the pink bollworm and histological changes in the neurosecretory cells of the brain during induction,maintenance and termination of diapause[J].Ann.Ent.Soc.Amer.,1978,71:375-382.
[244]Kind TV.Dynamics of activity of the brain neurosecretory cells in Pieris brassicae under the photoperiadic control of development and diapause of pupae[J].Zool.Zh.,1978,57:1668-1680.
[245]王方海,龚和.滞育和非滞育棉铃虫脑的组织解剖学研究[J].中山大学学报,1997,36(6):20-24.
[246]Kono Y.Ultrastructural changes of nourosecretory cells in the pars intercerebralis during diapause development in Pieris rapae[J].J.Insect Physiol.,1977,23:1461-1474.
[247]Wilson GR,Larsen JR.Debraining and diapause development in Manduca sexta pupae[J].J.Insect Physiol.,1974,20:2459-2474.
[248]McDaniel CN and Berry SJ.Activation of the prothoracic glands of Antheraea polyphemus[J].Nature,1967,214:1032-1034.
[249]Meola RW and Adkisson PL.Release of prothoracicotropic hormone and potentiation of developmental ability during diapause in the bollworm Heliothis zea[J].J.Insect Physiol.,1977,23:683-688.
[250]侯成林,徐卫华.棉铃虫促前胸腺激素的表达模式与发育调节[J].科学通报,2007,52(11):1269-1273.
[251]Khan MA.Brain-controlled synthesis of juvenile hormone in adult insects[J].Entomol.Exp.Appl.,1988,46:3-17.
[252]王方海,刘永平,张琼秀,等.蝗虫多型现象的神经内分泌调控[J].昆虫学报,2004,47(5):652-658.
[253]Hodkova M,Okuda T,Wagner RM.Regulation of corpora allata in females of Pyrrhocoris apterus(Heteroptera)[J].In Vitro Cellular & Developmental Biology-Animal,2001,37(9):560-563.
[254]Fukuda S.The production of diapause eggs by transplanting the suboesophageal ganglion in the silkworm[J].Proc.Jap.Acad.,1951,27:672-676.
[255]Fukuda S.Function of the pupal brain and suboesophageal ganglion in the production of non-diapause and diapause eggs in thesilkworm[J].Annt.Zool.Jap.,1952,25:149-155.
[256]Hasegawa K.Studies on the voltinism in the silkworm,Bombyx with special reference to organs containing determination of voltinism[J].Proc.Jap.Acad.,1951,27:667-671.
[257]吕鸿声.家蚕化性问题的生化遗传学研究.Ⅰ.家蚕化性的内分泌调节机制[J].蚕业科学通讯,1958,(1):11-22.
[258]吕鸿声.家蚕化性问题的生化遗传学研.Ⅱ.滞育激素与呼吸代谢及酶系活动[J].蚕业科学通讯,1959,(4):192-200.
[259]Hasegawa K.The diapause hormone of the silkworm,Bombyx mori[J].Nature,1957,179:1300-1301.
[260]Isobe M,Hasegawa K,Goto T.Isolation of the diapause hormone from the silkworm,Bombyx mori[J].J.Insect Physiol.,1973,19:1221-1239.
[261]Imai K,Konno T,Nakazawa Y,et aI.Isolation and structure of diapause hormone of the silkworm Bombyx mori[J].Proceedings of the Japan Academy,1991,67 B:98-101.
[262]Shiomi K,Ishida Y,Ikeda M,et al.Induction of non-diapause eggs by injection of anti-diapause hormone rabbit serum into the diapause type of the silkworm,Bombyx mori[J].J.Insect Physiol.,1994,40:693-699.
[263]Andrewartha HG,Miethke PM,Wells A.Induction of diapause in the pupa of Phalaenoides glycinae by a hormone from the suboesophageal ganglion[J].J.Insect Physiol.,1974,20:679-702.
[264]Hasegawa K.Studies in voltinism in the silkworm Bombyx mori L.with special reference to the organs concerning determination of voltinism[J].J..Fac.Agric.Tottori Univ.,1952,1:83-124.
[265]Sato Y,Oguchi M,Menjo N,et al.Precursor polyprotein for multiple neuropeptides secreted from the SG of the silkworm,Bombyx mori:Characterization of the cDNA encoding the diapause hormone precursor and identification of addition peptides[J].Proc.Nati.Acad.Sci.USA.,1993,90:3251-3255.
[266]徐卫华,佐藤行洋,山下兴亚.家蚕滞育激素的克隆[J].遗传学报,1995,22(3):178-182.
[267]Sato Y,Nakazawa Y,Menjo N,et al.A new diapause hormone molecular of the silkworm,Bombyx mori[J].Proc.Jap.Acad.,1992,68:75-79.
[268]Raina AK,Klun JA.Brain factor control of the sex pheromone production on the female corn earworm moth[J].Science,1984,225:531-533.
[269]徐卫华.家蚕滞育激素—信息素合成激活肽基因表达的调节[J].中国生物化学和分子生物学学报,1998,14(5):557-561.
[270]徐卫华.昆虫滞育的分子机理.Ⅱ:蛹期滞育激素基因表达与滞育决定[J].遗传学报,1999,26(2):107-111.
[271]Yamashita O,Hasegawa K.Oocyte age sensitive to the diapause hormone from the standpoint of glycogen synthesis in the silkworm,Bombyx mori[J].J.Insect Physiol.,1970,16:2377-2383.
[272]Xu WH,Sato Y,Ikeda M et al.Stage-dependent and temperature-controlled expression of the gene encoding the precursor protein of DH-PBAN in the silkworm,Bombyx mori[J].J.Biol.Chem.,1995,270:3804-3808.
[273]Xu WH,Zhang YQ,Wang Y,et al.Molecular mechanism of diapause in insect,1.Expression of diapause hormone gene and incubation temperature in embryonic stage of Bombyx mori[J].Science in China,1998,41(3):344-350.
[274]Pinyarat W,Shimada T,Xu WH,et al.Linkage analysis of the gene encoding the precursor protein of DH-PBAN in the silkworm,Bombyx mori[J].Genet.Res.Camb.,1995,65:105-111.
[275]Su ZH,Sato Y,Yamashita O.Purification,cDNA cloning and Northem blot analysis of trehalase of pupal midgut of the silkworm,Bombyx mori[J].Biochim.Biophys.Acta.,1993,1173:217-224.
[276]Su ZH,Ikeda M,Sato Y,et al.Molecular characterization of ovary trehalase of the silkworm,Bombyx mori and its transcriptional activation by diapause hormone[J].Biochim.Biophys.Acta.,1994,1218:366-374.
[277]Niimi T,Yamashita O,Yaginuma T.A cold-inducible Bombyx gene encoding a protein similar to mammalian sorbitol dehydrogenase[J].Eur.J.Biochem.,1993,213:1125-1131.
[278]张宾,沈晋良.棉铃虫滞育激素cDNA的克隆和序列测定[J].科学通报,1999,44(3):279-283.
[279]Sun JS,Zhang TY,Zhang QR,et al.Effect of the brain and suboesophageal ganglion on pupal development in Helicoverpa armigera through regulation of FXPRLamide neuropeptides[J].Reg.Peptides.,2003,116:163-171.
[280]Zhang TY,Sun JS,Zhang LB,et al.Cloning and expression of the cDNA encoding the FXPRL family of peptides and a functional analysis of their effect on breaking pupal diapause in Helicoverpa armigera[J].J.Insect Physiol.,2004,50:25-33.
[281]Sun JS,Zhang QR,Zhang TY,et al.Developmental expression of FXPRLamide neuropeptides in peptidergic neurosecretory cells of diapause-and nondiapause-destined individuals of the cotton bollworm,Helicoverpa armigera[J].General and Comparative Endocrinology,2005,141:48-57.
[282]汤清波,杨效文,郭线茹,等.烟夜蛾滞育激素的cDNA克隆和序列测定[J].河南农业大学学报,2001,35(4):303-307.
[283]丁矛,杨效文,郭线茹,等.烟夜蛾滞育激素基因在大肠杆菌中的表达[J].河南农业大学学报,2003,37(3):219-223.
[284]张蕾,杨效文,丁矛,等.烟夜蛾滞育激素基因时空表达的研究[J].河南农业大学学报,2004,38(1):36-40.
[285]Zhao JY,Xu WH,Kang L.Functional analysis of the SGNP I in the pupal diapause of the oriental tobacco budworm,Helicoverpa assulta(Lepidoptera:Noctuidae)[J].Regulatory Peptides,2004,118:25-31.
[286]徐春媛,刘彦群,鲁成.中国野桑蚕滞育激素基因的克隆与序列分析[J].蚕业科学,2003,29(3):236-240.
[287]Wilkins MR,Sanchez JC,Gooley AA,et al.Progress with proteome projects:why all proteins expressed by a genome should be identified and how to do it[J].Biotechnology and Genetic Engineering Reviews,1996,13:19-50.
[288]O'Farrell PH.High resolution two-dimensional electrophoresis of proteins[J].J.Biol.Chem.,1975,250(10):4007-4021.
[289]Bjellqvist B,Ek K,Righetti PG,et al.Isoelectric focusing in immobilized pH gradients:principle,methodology and some applications[J].J.Biochem Biophys Methods.,1982,6(4):317-339.
[290]Yates JR,Morgan SF,Gatlin CL.Method to compare collision-induced dissociation spetra of petides:potential for library searching and subtractive analysis[J].Anal.Chem.,1998,70:3557-3565.
[291]Wilkins MR,Gasteiger E,Bairoch A.Protein identification and analysis tools in the ExPASy server[J].Methods Mol.Biol.,1999,112:531-552.
[292]李学.药物蛋白质组学与药物研发[J].生理科学进展,2002,33(3):209-214.
[293]Giot L,Bader JS,Brouwer C,et al.A protein interaction map of Drosophila melanogaster[J].Science,2003,302(5651):1727-1736.
[294]Zdobnov EM,Mering C,Letunic I,et al.Comparative genome and proteome analysis of Anopheles gambiae and Drosophila melanogaster[J].Science,2002,298:149-159.
[295]McNall RJ,Adang MJ.Identification of novel Bacillus thuringiensis CrylAc binding proteins in Manduca sexta midgut through proteomic analysis[J].Insect Biochem.Mol.Biol.,2003,33(10):999-1010.
[296]Jurat-Fuentes JL,Adang MJ.Characterization of a CrylAc-receptor alkaline phosphatase in susceptible and resistant Heliothis virescens larvae[J].Eur.J.Biochem.,2004,271:3127-3135.
[297]Candas M,Loseva O,Oppert B,et al.Insect resistance to Bacillus thuringiensis:Alteration in the indianmeal moth larval gut proteome[J].Mol.Cell.Proteomics.,2003,2:19-28.
[298]刘凯于,杨红,蒋才富,等.抗苏云金杆菌Cry1Ac毒素粉纹夜蛾细胞系的特性研究[J].实验生物学报,2004,37(3):205-211.
[299]赵瑞君,李国锦,尹镭,等.溴氰菊酯在家蝇体内诱导蛋白的二维电泳分析[J].山西农业科学,1996,24(2):54-57.
[300]Sharma R,Komotsu S,Noda H.Proteomic analysis of brown planthopper:Application to the study of carbamate toxicity[J].Insect Biochem.Mol.Biol.,2004,34(5):425-432.
[301]Huang Z,Shi P,Dai J,et al.Protein metabolism in Spodoptera litura(F.)is influenced by the botanical insecticide azadirachtin[J].Pestic.Biochem.Physiol.,2004,80(2):85-93.
[302]KaeslinM,Pfister-Wilhelm R,Molina D,et al.Changes in the haemolymph proteome of Spodoptera littoralis induced by the parasitoid Chelonus inanitus or its polydnavirus and physiological implication[J].J.Insect Physiol.,2005,51(9):975-978.
[303]Wang YQ,Zhang PB,Fujii H,et al.Proteomic studies of lipolysaccharide-induced polypeptides in the silkworm,Bombyx mori[J].Biosci.Biotechnol.Biochem.,2004,68(8):1821-1823.
[304]Paskewitz SM,Shi L.The hemolymph proteome of Anopheles gambiae[J].Insect Biochem.Mol.Biol.,2005,35(8):815-824.
[305]吴琼,洪华珠.昆虫抗杆状病毒活性物质的诱导[J].病毒学报,2003,19(2):164-168.
[306]Levy F,Bulet P,Ehret-Saabatier L.Proteomic analysis of the systemic immune response of Drosophila[J].Mol.Cell.Proteomics.,2004,3:156-166.
[307]Loseva O,Engstrom Y.Analysis of signal-dependent changes in the proteome of Drosophila blood cells during an immune response[J].Mol.Cell.Proteomics.,2004,3:796-808.
[308]Karlsson C,Korayem AM,Scherfer C,et al.Proteomic analysis of Drosophila larval hemolymph[J].J.Biol.Chem.,2004,279(50):52033-52041.
[309]Engstrom Y,Loseva O,Theopold U.Proteomics of the Drosophila immune response[J].Trends Biotechnol.,2004,22:600-605.
[310]Vierstraete E,Verleyen P,Baggerman G,et al.A proteomic approach for the analysis of instantly released wound and immune proteins in Drosophila melanogaster hemolymph[J].PNAS,2004,101(2):470-475.
[311]姚国华,颜新培,钟伯雄.家蚕胚胎发育关联的初始蛋白质研究[J].蚕业科学,2004,30(4):436-439.
[312]Biron DG,Marche L,Ponton F,et al.Behavioural manipulation in a grasshopper harbouring hairworm:a proteomics approach[J].Proc.Biol.Sci.,2005,272:2117-2126.
[313]鲁华云,叶键,李建营,等.家蚕不同品种后部丝腺蛋白质双向电泳图谱比较[J].蚕业科学,2006,32(1):114-117.
[314]Hou Y,Xia Q,Zhao P,et al.Studies on middle silk gland and posterior-silk gland of silkworm(Bombyx mori)using two-dimensional electrophoresis and mass spectrometry[J].Insect Biochemistry and Molecular Biology,2007,37:486-496.
[315]Jia SH,Li MW,Zhou B,et al.Proteomic Analysis of Silk Gland Programmed Cell Death during Metamorphosis of the Silkworm Bombyx mori[J].Journal of Proteome Research,2007,(6):3003-3010.
[316]Li XH,Wu XF,Yue WF,et al.Proteomic Analysis of the Silkworm(Bombyx mori L.)Hemolymph during Developmental Stage[J].Journal of Proteome Research,2006,(5):2809-2814
[317]Uno Y,Fujiyuki T,Morioka M,et al.Identification of proteins whose expression is up-or down-regulated in the mushroom bodies in the honeybee brain using proteomics[J].FEBS Letters,2007,581:97-101.
[318]Schippers MP,Dukas R,Smith RW,et al.Lifetime performance in foraging honeybees:behaviour and physiology[J].J.Exp.Biol.,2006,209:3828-3836.
[319]陈巨莲,倪汉祥.小麦吸浆虫的研究进展[J].昆虫知识,1998,35(4):240-243.
[320]倪汉祥,丁红建,孙京瑞.小麦吸浆虫发生动态及综合治理对策[J].中国农学通报,1994,3(10):20-23.
[321]曾省.小麦吸浆虫[M].北京:农业出版社,1965.
[322]Kieffer JJ.Family Cecidomiidae[J].Genera Insectorum,1913,152:1-346.
[323]Barnes HF.Studies of fluctuations in insect population.Ⅹ.Prolonged larvae life and delayed subsequent emergence of the adult midge[J].J.Anim.Ecol.,1943,12:137-234.
[324]Barnes HF.Studies of fluctuations in insect population.Ⅻ.Further evidence of prolonged larval life in the wheat blossom midges[J].Ann.Appl.Biol.,1952,39:370-373.
[325]胡木林,张克斌.麦红吸浆虫滞育习性研究[J].昆虫知识,1995,32(1):13-16.
[326]刘家仁.小麦吸浆虫[J].农业科学通讯,1958,(4):215-217.
[327]曾省.小麦吸浆虫的生态地理、特性及其根治途径的讨论[J].中国农业科学,1962,3(3):10-15.
[328]Basedow T.The effect of temperature and precipitation on diapause and phenology in the wheat blossom midges Contarinia tritici(Kirby)and Sitodiplosis mosellana(Gehin)(Diptera:Cecidomyiidae)[J].Zoologische Jahrbucher Abteilung fur Systematik Okologie und Geographie der Tiere,1977,104(3):302-326.
[329]Hinks CF,Doane JF.Observations on rearing and diapause termination of Sitodiplosis mosellana(Diptera:Cecidomyiidae)in the laboratory[J].Journal of Economic Entomology,1988,81(6):1816-1818.
[330]胡木林.麦红吸浆虫滞育生理研究[D].西北农业大学硕士论文,1988.
[331]张克斌,胡木林.麦红吸浆虫滞育幼虫呼吸代谢的季节变化[J].西北农业大学学报,1988,16(增刊):30-34.
[332]仵均祥,袁锋,苏丽.麦红吸浆虫幼虫滞育期间糖类物质变化[J].昆虫学报,2004,47(2):178-183.
[333]仵均祥,袁锋,李怡萍.麦红吸浆虫幼虫滞育状态及其核酸含量变化研究[J].西北农林科技大学学报(自然科学版),2003,31(6):49-56.
[334]Wu JX,Yuan F.Changes of glycerol content in diapause larvae of the orange wheat blossom midge,Sitodiplosis mosellana(Gehin)in various seasons[J].Entomologia Sinica,2004,11(1):27-35.
[335]Wu JX,Yuan F,Zhang YM.Changes of fatty acids in larvae of the wheat blossom midge,Sitodiplosis mosellana(Gehin)(Diptera:Cecidomyiidae)[J].Entomologia Sinica,2001,8(4):315-322.
[336]侯娟娟,仵均祥,王洪亮.小麦吸浆虫不同滞育虫态的RAPD研究[J].西北农业学报,2006,15(4):10-13.
[337]王洪亮,仵均祥,成卫宁,等.用重复序列引物PCR分析不同滞育状态麦红吸浆虫DNA多态性[J].昆虫分类学报,2007,29(1):67-73.
[338]祝传书,袁锋.河北南和县2001年麦红吸浆虫发生成灾[J].植物保护,2002,28(2):60-61.
[339]袁峰,花保桢,仵均祥,等.小麦吸浆虫成灾规律与控制[M].北京:科学出版社,2004.
[340]Bradford MM.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye-binding[J].Analytical Biochemistry,1976,72:248-254
[341]Furusawa T,Narutaki A,Mitsuda K,et al.Stage-dependent limited degradation of vitellin and its localization in the Japanese oak silkworm,Antheraea yamamai,during embryonic development[J].Comp.Biochem.Physiol.,1993,104(4):787-794.
[342]Richards S,Liu Y,Bettencourt BR,et al.Comparative genome sequencing of Drosophila pseudoobscura:chromosomal,gene,and cis-element evolution[J].Genome Research,2005,15:1-18.
[343]Hofer-Warbinek R,Schmid JA,Mayer H,et al.A highly conserved proapoptotic gene,IKIP,located next to the APAF1 gene locus,is regulated by p53[J].Cell Death and Differentiation,2004,11:1317-1325.
[344]Seo Y J,Kwon MS,Choi SS,et al.Characterization of hypothalamic proopiomelanocortin gene and β-endorphin expression in the hypothalamic arcuate nucleus of mice elicited by inflammatory pain[J].Neuroscience,2008,152:1054-1066.
[345]Fridovich I.Oxygen is toxic[J].Bioscience,1977,27(7):462-467.
[346]冯从经,戴华国,武淑文.褐飞虱高温条件下应激反应及体内保护酶系活性的研究[J].应用生态学报,2001,12(3):409-413.
[347]马志卿,冯俊涛,江志利,等.松油烯-4-醇对家蝇超氧化物歧化酶、过氧化氢酶及过氧化物酶的影响[J].农药学学报,2004,6(2):53-56.
[348]张仙红,王宏民,李文英,等.菜青虫感染玫烟色拟青霉后血淋巴蛋白质含量及几种保护酶活力的变化[J].昆虫学报,2006,49(2):230-234.
[349]Grubor-Lajsic G,Block W,Telesmanic M,et al.Effect of cold acclimation on the antioxidant defense system of two larval Lepidoptera(Noctuidae)[J].Arch.Insect Biochem.Physiol.,1998,36(1):1-10.
[350]成卫宁,李修炼,李建军.夏季高温对翌年小麦吸浆虫发生的影响[J].西学农业学报,2002,11(4):13-15.
[351]方允中,李文杰.自由基与酶-基础理论及其在生物学和医学中的应用[M].北京:科学出版社,1989.
[352]Khani A,Moharramipour S,Barzega RM.Cold tolerance and trehalose accumulation in overwintering larvae of the codling moth,Cydia pomonella(Lepidoptera:Tortricidae)[J].Eur.J.Entomol.,2007,104(3):385-392.
[353]Joanisse DR,Storey KB.Enzyme activity profiles in an overwintering population of freeze tolerant larvae of the gall fly,Eurosta solidaginis[J].J.Comp.Physiol B.,1994,64:247-255.
[354]Rojas RR,Charlet LD,Leopold RA.Biochemistry and physiology of overwintering in the mature larvae of the sunflower stem weevil,Cylindrocopturus adspersus(Coleoptera:Curculionidae)in the northern great plains[J].J.Insect Physiol.,1994,40:201-205.
[355]李毅平,龚和,朴镐用.松针瘿蚊越冬幼虫体内酶活性的时序变化[J].昆虫学报,2000,43(3):227-232.
[356]Gelman DB,Woods CW.Haemolymph ecdysteroid titres of diapause and nondiapause-bound fifth instars and pupae of the European corn borer.Ostrinia nubilalis[J].Comp.Biochem.Physiol.,1983,76A:367-375.
[357]Takeda M,Matsumoto M,Tohno Y.Diapause intensity and ecdysiotroph:Comparisons between two Antheraea species having summer and winter diapause at pupae[J].Eur.J.Entomol.,1997,94:67-73.
[358]Lee KY,Denlinger DL.A role for ecdysteroids in the induction and maintenance of the pharate first instar diapause of the gypsy moth,Lymantria dispar[J].J.Insect Physiol.,1997,43(3):289-296.
[359]王满囷,李周直.鞭角华扁叶蜂蜕皮甾类激素滴度的变化[J].昆虫学报,2002,45(5):593-596.
[360]曹梅讯.20-羟基蜕皮酮的放射免疫分析法[J].昆虫知识,1986,32(2):87-90.
[361]王方海,张古忍,温瑞贞,等.入侵害虫蔗扁蛾幼虫期体内蜕皮激素的定性定量分[J].科学通报,2006,51(24):2870-2873.
[362]李英梅,仵均祥,成卫宁,等.麦红吸浆虫保幼激素含量测定[J].西北农业学报,2006,15(4):73-75.
[363]Suzuki K,Nakamura T,Yanbe T,et al.Termination of diapause in pharate first-instar larvae of the gypsy moth,Lymantria dispar japonica by an imidazole derivative KK-42[J].J.Insect Physiol.,1993,39(3):107-110.
[364]Danks HV.The role of insect cocoons in cold conditions[J].Eur.J.Entomol.,2004,101:433-437.
[365]Vollrath F.Strength and structure of spider silk[J].Rev.Mol.Biotechnol.,2000,74:67-83.
[366]Kubil S.High performance fibers from spidersilk[J].Angew.Chem.Int.Ed.Engl.,2002,41:2721-2723.
[367]高新起,王秀玲.荞麦子叶发育过程中聚合的粗面内质网[J].植物研究,2002,22(1):30-32.
[368]钟香臣,王宗舜,郭郛.家蚕后部丝腺细胞超微结构[J].昆虫学报,1980,23(3):342-348.
[369]戴玉锦.家蚕后部丝腺细胞粗面内质网膜系统的多态性研究[J].铁道师院学报,2001,18(3):21-25.