胃癌组织中PDCD5、COX-2和CD44的表达及其临床意义
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摘要
目的：通过检测PDCD5、COX-2和CD44在胃癌、胃炎和正常胃粘膜组织中的表达,分析PDCD5、COX-2和CD44的表达及与临床病理因素的相关性,探讨其在胃癌发生发展中的作用,为胃癌的诊断和判断预后提供一种理想的肿瘤标记物。同时探讨胃癌组织中PDCD5、COX-2和CD44三者表达的相关性。
方法：选取我院2003-2004年间手术切除并经病理证实、资料完整的32例胃癌组织标本、16例胃炎组织标本和12例正常胃粘膜组织标本的存档蜡块,应用组织芯片技术,通过免疫组化SABC法进行PDCD5、COX-2和CD44的检测,并对三者的表达情况及与胃癌临床病理特征的关系、三者在胃癌组织中表达的相关性进行统计分析。
结果：与胃炎和正常胃粘膜组织相比,在胃癌组织中COX-2和CD44的表达明显升高(P<0.05),而PDCD5的表达明显降低(P<0.05)；COX-2与胃癌细胞的侵润及淋巴结转移呈明显相关(P<0.05),但与胃癌组织分化程度及临床分期无相关性(P>0.05),而CD44与胃癌淋巴结转移、临床分期和侵润深度呈明显相关(P<0.05),但与胃癌的分化程度无相关性(P>0.05),三者的阳性表达与胃癌的部分恶性生物学行为有关。在有随访结果的32例胃癌患者中,生存分析显示,淋巴转移、临床分期、PDCD5、CD44及COX-2的表达均与预后有关(P<0.05),胃癌组织分化与预后无关(P>0.05)。
结论：COX-2、CD44在胃癌组织中过量表达伴随PDCD5表达缺失,与胃癌转移和侵润密切相关,三者可能存在共同的转录激活机制,构成了胃癌发生的多种途径,联合检测3种基因能更准确判断胃癌转移和侵润状态。
Objective The purpose of study was to explore the expression of PDCD5、COX-2 and CD44 in gastric cancer and chronic gastritis tissues,The expression of them was also detected in normal gastric tissues.With analysis of the relationships between the expression of PDCD5、COX-2 and CD44 and degrees of difrer-entiation,lymph node metastases and Borrmann stages.To investigate its possible mechanisms in the development and progression of gastric cancer.Aiming to find ideal tumor marker for diagnosis and prognosis.At the same time the correlation of expression of PDCD5、COX-2 and CD44 was explored.
Methods Using tissue microarray technique,in 32 stomach carcinoma tissues、16 chronic gastritis tissues and 12 normal gastric tissues,the expression of PDCD5、COX-2 and CD44 were examined by using streptavidin-biotin-peroxidase complex technique (SABC) method.All of the tissues were fixed in neutral formallin and enbedded in paraffin collected from 2003 to 2004.The correlation among these three genes was analyzed.
Results The expression levels of COX-2 and CD44 were upregulated (P<0.05),while the expression of PDCD5 downregulated(P<0.05) in gastric carcinoma compared with non-tumor tissues;the over-expression of COX-2 in gastric cancer was positively related to the metastasis and the depth of invasion (P<0.05),while it was not related with the histological types (P>0.05),and Borrmann types (P>0.05);the over-expression of CD44 in gastric cancer was obviously related to the metastasis、Borrmann types and the depth of invasion (P<0.05),while it was not related with the histological types (P>0.05), Upregulating expression levels of PDCD5 and CD44 were significance correlated with some malignant behaviour of gastric cancer.In the 32 cases with followed-up data,survi-val analysis reveal that the survival was correlated with invading of the metastasis Borrmann types and the expression of PDCD5、COX-2 and CD44.The histological grades were not related to prognosis.
Conclusion Patients who had over expression of COX-2 and CD44 and low expressi-on of PDCD5 have a higher invasion of tumor and lymph metastatic rate,it was speculated that may be a congenerous transcription mechanism with which to construct multiple pathways contributing to the development and progression of gastric cancer. Combined detection of three genes is a reliable index to evaluate the invasion and metastasis of gastric carcinoma.
方法：选取我院2003-2004年间手术切除并经病理证实、资料完整的32例胃癌组织标本、16例胃炎组织标本和12例正常胃粘膜组织标本的存档蜡块,应用组织芯片技术,通过免疫组化SABC法进行PDCD5、COX-2和CD44的检测,并对三者的表达情况及与胃癌临床病理特征的关系、三者在胃癌组织中表达的相关性进行统计分析。
结果：与胃炎和正常胃粘膜组织相比,在胃癌组织中COX-2和CD44的表达明显升高(P<0.05),而PDCD5的表达明显降低(P<0.05)；COX-2与胃癌细胞的侵润及淋巴结转移呈明显相关(P<0.05),但与胃癌组织分化程度及临床分期无相关性(P>0.05),而CD44与胃癌淋巴结转移、临床分期和侵润深度呈明显相关(P<0.05),但与胃癌的分化程度无相关性(P>0.05),三者的阳性表达与胃癌的部分恶性生物学行为有关。在有随访结果的32例胃癌患者中,生存分析显示,淋巴转移、临床分期、PDCD5、CD44及COX-2的表达均与预后有关(P<0.05),胃癌组织分化与预后无关(P>0.05)。
结论：COX-2、CD44在胃癌组织中过量表达伴随PDCD5表达缺失,与胃癌转移和侵润密切相关,三者可能存在共同的转录激活机制,构成了胃癌发生的多种途径,联合检测3种基因能更准确判断胃癌转移和侵润状态。
Objective The purpose of study was to explore the expression of PDCD5、COX-2 and CD44 in gastric cancer and chronic gastritis tissues,The expression of them was also detected in normal gastric tissues.With analysis of the relationships between the expression of PDCD5、COX-2 and CD44 and degrees of difrer-entiation,lymph node metastases and Borrmann stages.To investigate its possible mechanisms in the development and progression of gastric cancer.Aiming to find ideal tumor marker for diagnosis and prognosis.At the same time the correlation of expression of PDCD5、COX-2 and CD44 was explored.
Methods Using tissue microarray technique,in 32 stomach carcinoma tissues、16 chronic gastritis tissues and 12 normal gastric tissues,the expression of PDCD5、COX-2 and CD44 were examined by using streptavidin-biotin-peroxidase complex technique (SABC) method.All of the tissues were fixed in neutral formallin and enbedded in paraffin collected from 2003 to 2004.The correlation among these three genes was analyzed.
Results The expression levels of COX-2 and CD44 were upregulated (P<0.05),while the expression of PDCD5 downregulated(P<0.05) in gastric carcinoma compared with non-tumor tissues;the over-expression of COX-2 in gastric cancer was positively related to the metastasis and the depth of invasion (P<0.05),while it was not related with the histological types (P>0.05),and Borrmann types (P>0.05);the over-expression of CD44 in gastric cancer was obviously related to the metastasis、Borrmann types and the depth of invasion (P<0.05),while it was not related with the histological types (P>0.05), Upregulating expression levels of PDCD5 and CD44 were significance correlated with some malignant behaviour of gastric cancer.In the 32 cases with followed-up data,survi-val analysis reveal that the survival was correlated with invading of the metastasis Borrmann types and the expression of PDCD5、COX-2 and CD44.The histological grades were not related to prognosis.
Conclusion Patients who had over expression of COX-2 and CD44 and low expressi-on of PDCD5 have a higher invasion of tumor and lymph metastatic rate,it was speculated that may be a congenerous transcription mechanism with which to construct multiple pathways contributing to the development and progression of gastric cancer. Combined detection of three genes is a reliable index to evaluate the invasion and metastasis of gastric carcinoma.
引文
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