经穴与脏腑靶器官相关联聚焦性与弥散性功能效应特性规律的实验研究
|
摘要
目的:通过针刺背俞穴及其同名经原穴,探讨其对心脑靶器官特异性相关联的针刺效应规律和机理。
方法:在心肌梗死和脑出血大鼠模型的制备中,分别随机选取80只健康大鼠。把各模型80只大鼠随机分为对照组、模型组、心俞组、神门组、肾俞组、太溪组、承山组和非经非穴组,每组10只大鼠。除对照组为假手术组外,其他各组均制备相应的冠脉结扎心梗大鼠模型和Rosenberg法脑出血大鼠模型。在针刺治疗七天后观察各组大鼠以下指标:(1)心电图。(2)血清心肌酶学。(3)心肌组织和脑组织的NO、NOS和SOD。(4)血清IL—2和IL—6。(5)心肌组织和脑组织的MMP2和MMP9。通过对以上特异性指标的观察来探讨经穴是否对心脑靶器官有针刺效应及聚焦性与弥散性功能效应特性规律。
结果:在心肌梗死模型的心电图指标的观察中,与模型组比较心俞和神门组有显著差异,P分别＜0.01和0.05;在心肌酶学指标的观察中,与模型组比较心俞、神门和肾俞组有显著差异,除CK指标的神门组P＜0.05其他均＜0.01;在NO和NOS指标的观察中,与模型组比较心俞、神门和太溪组有显著差异,除NO指标的太溪组P＜0.05其他均＜0.01:在MMP2和MMP9指标的观察中,与模型组比较心俞、神门和肾俞组有显著差异,除MMP2指标的肾俞组P＜0.05其他均＜0.01。在脑出血模型IL—2和IL—6指标的观察中,与模型组比较心俞和肾俞组有显著差异,P＜0.05;在NO、NOS和SOD指标的观察中,与模型组比较心俞、肾俞和神门组有显著差异,除NOS指标的心俞组和SOD神门组P＜0.05其他均＜0.01;在MMP2和MMP9指标的观察中,与模型组比较心俞、肾俞和神门组有显著差异,除MMP9指标的心俞组P＜0.05其他均＜0.01。而在两模型的各个指标的观察中,与模型组比较承山组和非经非穴组均无显著差异。
结论:相对于承山组和非经非穴组,针刺背俞穴心俞及其同名经原穴神门对心脏靶器官有显著穴位特异性规律;而针刺肾俞穴及其同名经原穴太溪对心脏靶器官仅有一般(相对)穴位特异性规律。
相对于承山组和非经非穴组,针刺背俞穴心俞及肾俞穴对脑靶器官有显著穴位特异性规律:而针刺神门穴对脑靶器官仅有一般(相对)穴位特异性规律。
与承山穴和双非穴的各个指标观察对比来看,针刺背俞穴心俞和肾俞穴对心脑靶器官有比较鲜明的聚焦性与弥散性功能效应特性规律。
Objective:To research effect of acupuncture and mechanism of heart and brain associated with target organ specificity through acupuncturing back-shu point and the same meridian original-point.
Methods:In the rat model preparation of AMI and ICH,80 healthy rats were randomly selected distinctly.Both of the model were randomly assigned to the control group,model group,Xinshu group,shenmen group, Shenshu group,taixi Group,chengshan group and non-meridian-non-Point Group,each group has 10 rats.In addition to the control group in the sham-operated group,the other groups were prepared corresponding coronary artery ligation AMI model and Rosenberg ICH model.After seven days in the acupuncture treatment,each group rats were observed in following indicators:(1)ECG.(2)serum myocardial enzyme.(3) myocarditis and brain tissue NO,NOS and SOD.(4)serum IL-2 and IL-6. (5)myocarditis and brain tissue MMP2 and MMP9.Through the observation of mention-aboved specific indicators,to explore whether meridian point has law of acupuncture effects and focus or diffuse function effects to heart and brain target organ.
Results:In ECG indicators of AMI model,compared with the model group, Xinshu and shenmen group have significant difference,respectively P＜0.01 and 0.05;in NO and NOS indicators,compared with the model group, Xinshu,shenmen and taixi Group have significant difference,in addition to CK indicators shenmen group P＜0.05 all other＜0.01;in MMP2 and MMP9 indicators,compared with the model group,Xinshu,shenmen and shenshu guoup have significant difference,in addition to MMP2 indicators shenshu group P＜0.05 all other＜0.01.In IL-2 and IL-6 indicators of ICH model,compared with the model group,Xinshu and shenshu group have significant difference,P＜0.05;in NO,NOS and SOD indicators, compared with the model group,Xinshu,shenshu and shenmen group have significant difference,in addition to NOS xinshu group and SOD shenmen group P＜0.05 all other＜0.01.In MMP2 and MMP9 indicators,compared with the model group,Xinshu,shenshu and shenmen group have significant difference,in addition to MMP9 indicators xinshu group P＜0.05 all other＜0.01.Than the two models in the various indicators of observation, compared with the model group,chengshan group and non-meridian-non-Point Group have no significant difference.
Conclusions:Compared with chengshan group and non-meridian-non-Point Group,acupuncturing xinshu of back-shu point and shenmen of the same meridian original-point have significant point-specificity law to heart target organ,but shenshu and the same meridian original-point have the only general(relative)point-specificity law to heart target organ. Compared with chengshan group and non-meridian-non-Point Group, acupuncturing xinshu and shenshu of back-shu point have significant point-specificity law to brain target organ,but shenmen point has the only general(relative)point-specificity law to brain target organ.And the chengshan group and non-meridian-non-Point Group observation comparison of the various indicators,acupuncturing xinshu and shenshu points of back-shu point have a clear focus and diffuse functional effects of law to heart and brain target organs.
方法:在心肌梗死和脑出血大鼠模型的制备中,分别随机选取80只健康大鼠。把各模型80只大鼠随机分为对照组、模型组、心俞组、神门组、肾俞组、太溪组、承山组和非经非穴组,每组10只大鼠。除对照组为假手术组外,其他各组均制备相应的冠脉结扎心梗大鼠模型和Rosenberg法脑出血大鼠模型。在针刺治疗七天后观察各组大鼠以下指标:(1)心电图。(2)血清心肌酶学。(3)心肌组织和脑组织的NO、NOS和SOD。(4)血清IL—2和IL—6。(5)心肌组织和脑组织的MMP2和MMP9。通过对以上特异性指标的观察来探讨经穴是否对心脑靶器官有针刺效应及聚焦性与弥散性功能效应特性规律。
结果:在心肌梗死模型的心电图指标的观察中,与模型组比较心俞和神门组有显著差异,P分别＜0.01和0.05;在心肌酶学指标的观察中,与模型组比较心俞、神门和肾俞组有显著差异,除CK指标的神门组P＜0.05其他均＜0.01;在NO和NOS指标的观察中,与模型组比较心俞、神门和太溪组有显著差异,除NO指标的太溪组P＜0.05其他均＜0.01:在MMP2和MMP9指标的观察中,与模型组比较心俞、神门和肾俞组有显著差异,除MMP2指标的肾俞组P＜0.05其他均＜0.01。在脑出血模型IL—2和IL—6指标的观察中,与模型组比较心俞和肾俞组有显著差异,P＜0.05;在NO、NOS和SOD指标的观察中,与模型组比较心俞、肾俞和神门组有显著差异,除NOS指标的心俞组和SOD神门组P＜0.05其他均＜0.01;在MMP2和MMP9指标的观察中,与模型组比较心俞、肾俞和神门组有显著差异,除MMP9指标的心俞组P＜0.05其他均＜0.01。而在两模型的各个指标的观察中,与模型组比较承山组和非经非穴组均无显著差异。
结论:相对于承山组和非经非穴组,针刺背俞穴心俞及其同名经原穴神门对心脏靶器官有显著穴位特异性规律;而针刺肾俞穴及其同名经原穴太溪对心脏靶器官仅有一般(相对)穴位特异性规律。
相对于承山组和非经非穴组,针刺背俞穴心俞及肾俞穴对脑靶器官有显著穴位特异性规律:而针刺神门穴对脑靶器官仅有一般(相对)穴位特异性规律。
与承山穴和双非穴的各个指标观察对比来看,针刺背俞穴心俞和肾俞穴对心脑靶器官有比较鲜明的聚焦性与弥散性功能效应特性规律。
Objective:To research effect of acupuncture and mechanism of heart and brain associated with target organ specificity through acupuncturing back-shu point and the same meridian original-point.
Methods:In the rat model preparation of AMI and ICH,80 healthy rats were randomly selected distinctly.Both of the model were randomly assigned to the control group,model group,Xinshu group,shenmen group, Shenshu group,taixi Group,chengshan group and non-meridian-non-Point Group,each group has 10 rats.In addition to the control group in the sham-operated group,the other groups were prepared corresponding coronary artery ligation AMI model and Rosenberg ICH model.After seven days in the acupuncture treatment,each group rats were observed in following indicators:(1)ECG.(2)serum myocardial enzyme.(3) myocarditis and brain tissue NO,NOS and SOD.(4)serum IL-2 and IL-6. (5)myocarditis and brain tissue MMP2 and MMP9.Through the observation of mention-aboved specific indicators,to explore whether meridian point has law of acupuncture effects and focus or diffuse function effects to heart and brain target organ.
Results:In ECG indicators of AMI model,compared with the model group, Xinshu and shenmen group have significant difference,respectively P＜0.01 and 0.05;in NO and NOS indicators,compared with the model group, Xinshu,shenmen and taixi Group have significant difference,in addition to CK indicators shenmen group P＜0.05 all other＜0.01;in MMP2 and MMP9 indicators,compared with the model group,Xinshu,shenmen and shenshu guoup have significant difference,in addition to MMP2 indicators shenshu group P＜0.05 all other＜0.01.In IL-2 and IL-6 indicators of ICH model,compared with the model group,Xinshu and shenshu group have significant difference,P＜0.05;in NO,NOS and SOD indicators, compared with the model group,Xinshu,shenshu and shenmen group have significant difference,in addition to NOS xinshu group and SOD shenmen group P＜0.05 all other＜0.01.In MMP2 and MMP9 indicators,compared with the model group,Xinshu,shenshu and shenmen group have significant difference,in addition to MMP9 indicators xinshu group P＜0.05 all other＜0.01.Than the two models in the various indicators of observation, compared with the model group,chengshan group and non-meridian-non-Point Group have no significant difference.
Conclusions:Compared with chengshan group and non-meridian-non-Point Group,acupuncturing xinshu of back-shu point and shenmen of the same meridian original-point have significant point-specificity law to heart target organ,but shenshu and the same meridian original-point have the only general(relative)point-specificity law to heart target organ. Compared with chengshan group and non-meridian-non-Point Group, acupuncturing xinshu and shenshu of back-shu point have significant point-specificity law to brain target organ,but shenmen point has the only general(relative)point-specificity law to brain target organ.And the chengshan group and non-meridian-non-Point Group observation comparison of the various indicators,acupuncturing xinshu and shenshu points of back-shu point have a clear focus and diffuse functional effects of law to heart and brain target organs.
引文
[1]邓春雷,殷克敬.实验针灸学[M].北京:人民卫生出版社,1998:245.
[2]范黎,徐明海,陈修贵.不同腧穴的神经在脊髓胶状质的定位[J].上海针灸杂志[J],1999,18(1):36-37.
[3]Langevin HM,Yandow JA.Relationship of acupuncture points and meridians to connective tissue planes[J]the Anatomical Record,2002,269(6):257-265.
[4]费伦,承焕生,蔡德亨等.经络物质基础及其功能性特征的实验探索和研究展望[J].科学通报,1998,43(6):658-673.
[5]韩兆亮.人体双侧对称经络电阻抗失衡与疾病的相关研究[J].菏泽医专学报,1999,11(1):9.
[6]周钰,王捷生,沈雪勇.经穴伏安特性研究及其临床应用前景[J].上海针灸杂志,2005,24(3):30.
[7]程绍鲁,郑毓新,余遒登等.443例人体腧穴皮肤电阻抗振荡波动现象观察[J].针刺研究,2000,25(2):109.
[8]李万瑶,小川秀道,吉田肇.经穴皮电在不同手术麻醉中的变化[J].中国针灸,1994,14(6):33.
[9]郑建.妇女月经周期中任、督脉经穴的电学特性[J].中国针灸,1998,18(7):401.
[10]杨跃平,李启林.物理学在针灸经穴研究中的应用[J].张家口医学院学报,1996,13(1):76.
[11]吕越,部分疾病与背部腧穴超微弱发光的强度的实验研究[J].陕西中医学院学报,1997,20(7):43.
[12]李定忠等.中医经络探秘[M].北京:解放军出版社,2003.
[13]于建春等.从基因表达差异分析腧穴和非腧穴针刺效应差异[J].中国针灸,2002,22(11):749-751.
[14]李江山,严洁,常小荣.电针足阳明经穴对家兔胃运动功能影响的腧穴特异性研究[J].湖南中医学院学报,2004,24(3):43.
[15]余明哲等.针刺治疗十二指肠溃疡的腧穴特异性研究[J].上海针灸杂志,2001,120(5)
[16]袁其伦.腧穴实质与脏腑相关的新认识和新应用[J].中国针灸,2004,24(6):423-425.
[17]童晨光,衣华强,谷世吉,等.小肠俞募穴与小肠特异性联系通路的荧光双标法研究[J].南京中医药大学学报,2003,19(1):41-43.
[18]童晨光.肾俞募穴与肾特异性联系通路的荧光双标法研究[J].针刺研究,2002,27,(4):248-252.
[19]童晨光.胃俞募穴与胃特异性联系通路的荧光双标法研究[J].上海针灸杂志2003,22(5):16-19.
[20]黄泳等.针刺百会人中神门治疗血管性痴呆的临床比较研究[J].云南中医中药杂志,2005,26(1):41-42.
[21]吕景山,吕玉娥,吕运权.穴对应用经验辑要[J].中国针灸,2002,22(5):321-322.
[22]张力,伍松合,黄小明等.电针足三里和内关在术后胃肠功能恢复中的作用观察[J].广西中医药,2001,24(4).36-37.
[23]罗海鸥,唐勇,陈瑾等.中脘胃俞配伍针刺对消化性溃疡患者血浆的影响[J].针灸临床杂志,2003,19(4):48.
[24]王光义,蒋乃昌.电针急性高血压大鼠降压效果应用及探讨[J].中国针灸,1997,17(2):105.
[25]冀来喜,燕平.腧穴组方对胃粘膜损伤大鼠胃粘膜保护作用的研究.中国针灸,2002,22(7):467-469.
[26]孙维仁.背俞理论探索与临床应用:内蒙古人民出版社,1994,3,4.
[27]王佩,刘公望,易受乡等.经穴-脏腑相关的神经节段机制.见:林文注,王佩主编.实验针灸学[M].上海科技出版社,1999:48.
[28]童晨光,谷世喆,衣华强.胸腹气街的形态学基础[J].针刺研究,2004,29(4):270-273.
[29]汪克明,周美启,王月兰等.电针“脾俞”对胃窦部溃疡大鼠胃肠平滑肌电活动的干预作用及其机制探讨[J].安徽中医学院学报,2003,22(6):29-31..
[30]段延萍,周杰等.背俞穴临证举隅[J].陕西中医,2006,27(2):217.
[31]徐斯伟.背俞穴为主治疗哮喘的疗效观察[J].上海针灸杂志,2007,26(10):9-10.
[32]郑祖艳,朱崇霞.针刺背俞穴治疗焦虑症的临床研究[J].辽宁中医学院学报,2004,6(3):208.
[33]夏春发.针刺背俞穴治疗老年性便秘45例[J].陕西中医,2006,27(1):95-96.
[34]陈惠玲.挑刺背俞穴治疗面部痤疮体会[J].中外健康文摘,2007,4(6):66-67.
[35]厉卫红.背俞穴应用与针灸整体美容思想[J].辽宁中医药大学学报2006,8(5).
[36]灵枢[M].北京:人民卫生出版社,2000,3:114-118,129-130.
[37]林蕙兰,马杜古.从穴温探索内脏与体表的联系[J].新中医,1982,1:30-32.
[38]李婷,陈小兵.电针太白穴对实验性脾虚家兔的影响[J].中国中医药信息杂志,1999,6(5):25-26.
[39]严智强,田力,林伟力等.人体十二经脉穴位冷光规律的探讨[J].中国针灸,1984,2:24-26.
[40]王少军.井原穴皮温差的临床观察[J].辽宁中医杂志,1995,22(4):178.
[41]赵力茹等.中国针灸[M].1988,4:44.
[42]高武.针灸聚英[M].上海科学技术出版社,1987.
[43]张永臣,吴富东.四关和四关穴探析[J].针灸临床杂志,2005,21,(6):5-6.
[44]邹敏,刘洁,唐勇.针刺四关穴治疗偏头痛及其对一氧化氮水的影响[J].中国中医急症,2003,12(2):129.
[45]熊明.四关穴临床应用体会[J].实用中医药杂志,2006,22(5):300.
[46]成泽东.原穴与脏腑同源性理论探析[J].辽宁中医杂志,2006,33(5):541.
[47]徐晓红,郑鹏.再论俞原穴及其配伍方法[J].长春中医学院学报,2004,20(12):17.
[48]黄东挺.原俞配穴针灸综合治疗失眠症68例[J].针灸临床杂志,2004,20(6):44-45.
[49]郭闰萍.浅析原穴的配伍规律[J].黑龙江中医药,2004,6:39.
[50]闵学进.原穴在急症中的配伍应用[J].针灸临床杂志,1997,13(11):41.
[51]刘俊岭,陈振荣,胡翔龙,荣培晶.2000年度经络研究进展[J].针刺研究,2001,26(1):72-76.
[52]胡翔龙.中国经络研究十年[J].中国针灸,1999,(7):389.
[53]蒋大宗.对经络实质的一种猜想及开展研究的建议[J].中国医疗器械杂志,2002,26(4):235-236..
[54]蒋正生.对经络实质的理论探讨[J].针灸临床杂志.1997,13(6):15-17.
[55]张锡钧.“经络,皮层,内脏相关”假说.上海中医学院编.针灸学,人民卫生出版社,北京,1974,100.
[56]杨志永,张铁红.经络实质的假说及临床验证[J].中国针灸,188.
[57]李树德,高渌汶.经络理论新探[J].中国针灸,188.
[58]罗汀,郭义,王秀云.经络循行生化研究近况[J].上海针灸杂志,2006,25(6):45-47.
[59]郭义,张艳军,苗文方,等.钙离子是经络活动的关键因素之一[J].中国中医基础医学杂志,1998,4(7):49.
[60]郭义.家兔经穴钙离子浓度特异性的研究[J].针刺研究,1991,16(1):66.
[61]郭义.络合足三里穴 Ca2+对针刺足三里抑制正常家兔胃运动影响的实验研究[J].针刺研究,1998,23(2):146.
[62]章汉平.经络深层组织pH环境特点探讨[C].第七届全国经络学术会议论文选编,1993.
[63]成柏华,李忠华,杨红英.针刺不同经络对穴位内K~+、Na~+、Ca2+、H~+的动态变化观察[J].上海针灸杂志,1994,13(2):80-81.
[64]黄改娣.针刺镇痛与局部pH值关系的研究[C].中国针灸学会实验研究会第五次学术交流会论文摘要选编,1990,104.
[65]郭义,张艳军,苗文方,等.实验性心律失常家兔内关穴H~+ 浓度的变化的动态监测研究[J].中国中医药科技,1994,1(6):12.
[66]王秀云,郭义,张艳军,等.针刺对家兔腧穴K~+浓度的影响[J].中国中医基础医学杂志,1998,4(1):48.
[67]刘万成,孔早鸣,张晓彬.针刺家兔“阴市”穴对“伏兔”穴区肌肉组织钾、钠、钙离子含量的影响[J].黑龙江中医药,1986,2(2):33.
[68]谢远军,胡翔龙,吴宝华.经脉循行线上组织氧分压的观察及其针刺的影响[J].中国中医药科技,2000,7(6):353.
[69]费伦,承焕生,蔡德亨,等.经络物质基础及其功能性特征的实验探索和研究展望[J].科学通报,1998,43(6):866.
[70]上海经络研究所.针刺可改变穴位组织的锌含量[J].上海中医情报,1986,(3):4.
[71]邵正一,凌祥.“内关”、“足三里”注射胰岛素后对健康家兔部分外周脉线及穴位微量元素的影响[J].中国针灸,1996,16(6):37.
[78]徐淑云.药理实验方法学.第2版,北京:人民卫生出版社,1994.
[79]华兴邦.大鼠穴位图谱的研究[M].实验动物与动物实验.1991, (1):1-5.
[80]Rosenberg CA,Mun-bryce S,Wesley M,et al.Collagenase-induced intracerebral hemorrhage in rats.Stroke,1990,21:801.
[81]刘立公,顾杰,沈雪勇等.古代文献中膀胱经及其腧穴主治的统计报告[J].上海针灸杂志,2004,23(1 2):42.43.
[82]严健民.论足太阳膀胱经在经脉学说中的历史地位[J]中国中医基础医学杂志,2003,9(11):857-859.
[83]胡芝兰.脏腑背俞穴刍议[J].浙江中医学院学报,1994,18(3):47-48.
[84]陈树林,李育良.内关、心俞与心脏相关的神经基础[J].中国针灸,1996,(11):676-678.
[85]衣华强.心俞募穴与心脏特异性联系通路的荧光双标法研究[J].针刺研究,2002,27(4):245-248.
[86]尉迟静.有关心俞穴入心路线的观察[J].北京中医杂志,1986,(4):47.
[87]陈少宗.针刺神门穴对冠心病患者左心功能的即时影响[J].针灸学报,1993,(1):20-22.
[88]孙宝彩等.针刺神门治疗室上性阵发性及窦性心动过速[J].山东中医杂志,1995,14(10):471.
[89]尹改珍,徐世芬.隋·巢元方“灸背俞治五脏中风”说考析[J].中医药学刊,2003,21,(7):1134.
[90]符文彬,谢金华.司徒铃教授运用背俞穴治病经验[J].北京中医药大学学报,1995,18,(4):52-53.
[91]盛燮荪,杭宇飞.孙思邈应用背俞穴经验浅析[J].陕西中医,2000,21(5):233.
[92]徐以增等.五脏背俞穴用于中风治疗探析[J].四川中医,1998,16,(7):50-51.
[93]郁云亚.上海针灸杂志,1993,(2):54.
[94]郑宗昌,等,中国针灸,1995,(4):18.
[95]何涛,蒙华,宋风卿.105只健康大鼠心电图分析[J].JOURNAL OF PRACTICAL ELECTROCARDIOLOGY,2006,15,(4):89-290.
[96]Yamamoto S Matsui K,Sasabe M et al.effect of an o-rally active Na~+/H~+exchange inhibitor,SMP-300 experimental angina and myocardial infarction models rats.J Cardiovase Pharmacol,2002,39(2):234-241.
[97]赵瑞等.鼠实验性心肌缺血模型的心电图动态监测及分析[R].第二届中药现代化新剂型新技术国际学术会议,2006:500-506.
[98]张晋,王晓良.内皮源性血管活性因子的研究进展[J].中国约理学通报,2006,16,(1):11.
[99]王吉云.一氧化氮的病理生理作用的新观点[J].国外医学心血管疾病分册,2004,31,(1):33.
[100]Matheis G.Role of larginine-nitric oxide pathway in myocardial rcoxy-genationinjury.Am J Physical,1992,262(2):H616.
[101]Small DL,Buchan AM.Mechanisms of Cerebral Ischcmia:Intracellular Cascades and Therapeutic Interventions.J Car-diothorac Vase Ancsth,1996,10:139.
[102]姜亚军,何家声,周君富.脑出血患者氧化抗氧化状态研究[J].铁道医学.1997,25(6):335.
[103]Beckman JS,KoppenolWH.Nitric oxide,super ox-ide,and peroxynitritE:the good,the bad,and ugly[J].Am J Phsiol-Cell Physio,1 1996,271:1424-1437.
[104]刘 欣,黄作义,王复新.大鼠脑出血周边组织NO含量和NOS活性变化及对细胞凋亡的影响[J].黑龙江医药科学,2004,27(3):1-3.
[105]Peeling,J,Yan HJ,Chen SG,et al.Protective effects of free radical inhibitors in intracerebral hemorrhage in rats[J].Brain Res,1998,795(1-2):63-70.
[106]任泽光,吴建中,尹可.高血压脑出血后脑血流及自由基变化规律及其与脑水肿的关系[J].中华神经外科杂志,1995,11(4):209-212.
[107]Dorsch NW.Hemorrhagic stroke.Intracerebral and subarachnoid hemor-rhage (J).Aust Fam Physician,1997,26(10):1145-1150.
[108]Matsuo Y,Kihara T,lkedaM,et al.Role of neutrophils in radical pro-duction during ischemia and reperfusion of the rat brain:effect of neu-trophil depletion on extracellular ascorbyl radical formation(J)· J CerebBlood FlowMetab,1995,15(6):941-947.
[109]John,W Pterson,Byung-Dulk,et al.Immunologicalreaction against the aging human subarachnoid ery-throcyte[J].J Neurosurg,1989,71:718-726.
[110]Jan Ake Lindgren,TomasHokfelt,et al.Leukotrienes in the rat central nervous system.ProeNailAeadSeiUSA,1984,81:6212-6216.
[111]A-L Hulting,et al.LeukotrieneC4 as a mediator luteinizing hormone re-lease from rat anterior Pituitary cells.Proc Natl Acad.Sci.USA,1988,82:3834.
[112]Muraguchi A,Hirano T,Tang B,et al.The essential role of B cell stimulatory factor 2(BSF-2/IL-6)for the terminal differentation of B celI.JEXP MED,1998.166:33
[113]Broish L,Rostenbaum R,Albury l,et al.Activation of neurophils by interleukin-6.Cell Immunol,1989,121:280
[114]Tarkowski E,Rosengren L,Blomstrnd C,et al.Early mtratheeal Produetion interleukin-6 Prediets the size of brain lesion in stroke.Stroke,1996.27:1553
[115]郭云等.实验性大鼠脑出血急性期血浆及脑组织中白介素6的含量[J].第四军医大学学报,2001,22(25):1409-1411.
[116]杨巧莲等.脑出血患者血清IL-6含量动态变化研究[J].卒中与神经疾病,2000,7(4):233-234.
[117]Visse R,Nagase H.Matrix metalloproteinases and tissue inhibitors of metal-loproteinases.Circ Res,2003,92:827-839.
[118]Siwik DA,Colucci WS.Regulation of matrix metalloproteinases by Cytokines and reactive oxygen/nitrogen species in the my-ocardium[J].Heart Fail Rev,2004,9(1):43.
[119]Kai H,Ikeda H,Yasukawa H et al.Peripheral blood levels of matrix metallo-proteinases-2 and-9 are elevated in patients with acute coronary syndromes.J Am Coll Cardiol,1998,32:368-372.
[120]Romanic AM,Burns-Kurtis CL,Gout B,et al.Matrix metalloproteinase expression in cardiac myocytes following myocardial infarction in the rab-bit.Life Sci,2001,68(7):799-814.
[121]Hojo Y,Ikeda U,Ueno S,et al.Expression of matrix metalloproteinase in patients with acute myocardial infarction[J].Jpn Cite J,2001,65(2):71-75.
[122]Rosenberg GA.Matrix metalloproteinases in neuroinflamation[J].Glia,2002,8(39):279-283.
[123]田力,刘海峰,滕伟禹.大鼠实验性脑出血后基质金属蛋白酶 2 和 9 的表达[J].中国动脉硬化杂志,2006,14(3):221-223.
[2]范黎,徐明海,陈修贵.不同腧穴的神经在脊髓胶状质的定位[J].上海针灸杂志[J],1999,18(1):36-37.
[3]Langevin HM,Yandow JA.Relationship of acupuncture points and meridians to connective tissue planes[J]the Anatomical Record,2002,269(6):257-265.
[4]费伦,承焕生,蔡德亨等.经络物质基础及其功能性特征的实验探索和研究展望[J].科学通报,1998,43(6):658-673.
[5]韩兆亮.人体双侧对称经络电阻抗失衡与疾病的相关研究[J].菏泽医专学报,1999,11(1):9.
[6]周钰,王捷生,沈雪勇.经穴伏安特性研究及其临床应用前景[J].上海针灸杂志,2005,24(3):30.
[7]程绍鲁,郑毓新,余遒登等.443例人体腧穴皮肤电阻抗振荡波动现象观察[J].针刺研究,2000,25(2):109.
[8]李万瑶,小川秀道,吉田肇.经穴皮电在不同手术麻醉中的变化[J].中国针灸,1994,14(6):33.
[9]郑建.妇女月经周期中任、督脉经穴的电学特性[J].中国针灸,1998,18(7):401.
[10]杨跃平,李启林.物理学在针灸经穴研究中的应用[J].张家口医学院学报,1996,13(1):76.
[11]吕越,部分疾病与背部腧穴超微弱发光的强度的实验研究[J].陕西中医学院学报,1997,20(7):43.
[12]李定忠等.中医经络探秘[M].北京:解放军出版社,2003.
[13]于建春等.从基因表达差异分析腧穴和非腧穴针刺效应差异[J].中国针灸,2002,22(11):749-751.
[14]李江山,严洁,常小荣.电针足阳明经穴对家兔胃运动功能影响的腧穴特异性研究[J].湖南中医学院学报,2004,24(3):43.
[15]余明哲等.针刺治疗十二指肠溃疡的腧穴特异性研究[J].上海针灸杂志,2001,120(5)
[16]袁其伦.腧穴实质与脏腑相关的新认识和新应用[J].中国针灸,2004,24(6):423-425.
[17]童晨光,衣华强,谷世吉,等.小肠俞募穴与小肠特异性联系通路的荧光双标法研究[J].南京中医药大学学报,2003,19(1):41-43.
[18]童晨光.肾俞募穴与肾特异性联系通路的荧光双标法研究[J].针刺研究,2002,27,(4):248-252.
[19]童晨光.胃俞募穴与胃特异性联系通路的荧光双标法研究[J].上海针灸杂志2003,22(5):16-19.
[20]黄泳等.针刺百会人中神门治疗血管性痴呆的临床比较研究[J].云南中医中药杂志,2005,26(1):41-42.
[21]吕景山,吕玉娥,吕运权.穴对应用经验辑要[J].中国针灸,2002,22(5):321-322.
[22]张力,伍松合,黄小明等.电针足三里和内关在术后胃肠功能恢复中的作用观察[J].广西中医药,2001,24(4).36-37.
[23]罗海鸥,唐勇,陈瑾等.中脘胃俞配伍针刺对消化性溃疡患者血浆的影响[J].针灸临床杂志,2003,19(4):48.
[24]王光义,蒋乃昌.电针急性高血压大鼠降压效果应用及探讨[J].中国针灸,1997,17(2):105.
[25]冀来喜,燕平.腧穴组方对胃粘膜损伤大鼠胃粘膜保护作用的研究.中国针灸,2002,22(7):467-469.
[26]孙维仁.背俞理论探索与临床应用:内蒙古人民出版社,1994,3,4.
[27]王佩,刘公望,易受乡等.经穴-脏腑相关的神经节段机制.见:林文注,王佩主编.实验针灸学[M].上海科技出版社,1999:48.
[28]童晨光,谷世喆,衣华强.胸腹气街的形态学基础[J].针刺研究,2004,29(4):270-273.
[29]汪克明,周美启,王月兰等.电针“脾俞”对胃窦部溃疡大鼠胃肠平滑肌电活动的干预作用及其机制探讨[J].安徽中医学院学报,2003,22(6):29-31..
[30]段延萍,周杰等.背俞穴临证举隅[J].陕西中医,2006,27(2):217.
[31]徐斯伟.背俞穴为主治疗哮喘的疗效观察[J].上海针灸杂志,2007,26(10):9-10.
[32]郑祖艳,朱崇霞.针刺背俞穴治疗焦虑症的临床研究[J].辽宁中医学院学报,2004,6(3):208.
[33]夏春发.针刺背俞穴治疗老年性便秘45例[J].陕西中医,2006,27(1):95-96.
[34]陈惠玲.挑刺背俞穴治疗面部痤疮体会[J].中外健康文摘,2007,4(6):66-67.
[35]厉卫红.背俞穴应用与针灸整体美容思想[J].辽宁中医药大学学报2006,8(5).
[36]灵枢[M].北京:人民卫生出版社,2000,3:114-118,129-130.
[37]林蕙兰,马杜古.从穴温探索内脏与体表的联系[J].新中医,1982,1:30-32.
[38]李婷,陈小兵.电针太白穴对实验性脾虚家兔的影响[J].中国中医药信息杂志,1999,6(5):25-26.
[39]严智强,田力,林伟力等.人体十二经脉穴位冷光规律的探讨[J].中国针灸,1984,2:24-26.
[40]王少军.井原穴皮温差的临床观察[J].辽宁中医杂志,1995,22(4):178.
[41]赵力茹等.中国针灸[M].1988,4:44.
[42]高武.针灸聚英[M].上海科学技术出版社,1987.
[43]张永臣,吴富东.四关和四关穴探析[J].针灸临床杂志,2005,21,(6):5-6.
[44]邹敏,刘洁,唐勇.针刺四关穴治疗偏头痛及其对一氧化氮水的影响[J].中国中医急症,2003,12(2):129.
[45]熊明.四关穴临床应用体会[J].实用中医药杂志,2006,22(5):300.
[46]成泽东.原穴与脏腑同源性理论探析[J].辽宁中医杂志,2006,33(5):541.
[47]徐晓红,郑鹏.再论俞原穴及其配伍方法[J].长春中医学院学报,2004,20(12):17.
[48]黄东挺.原俞配穴针灸综合治疗失眠症68例[J].针灸临床杂志,2004,20(6):44-45.
[49]郭闰萍.浅析原穴的配伍规律[J].黑龙江中医药,2004,6:39.
[50]闵学进.原穴在急症中的配伍应用[J].针灸临床杂志,1997,13(11):41.
[51]刘俊岭,陈振荣,胡翔龙,荣培晶.2000年度经络研究进展[J].针刺研究,2001,26(1):72-76.
[52]胡翔龙.中国经络研究十年[J].中国针灸,1999,(7):389.
[53]蒋大宗.对经络实质的一种猜想及开展研究的建议[J].中国医疗器械杂志,2002,26(4):235-236..
[54]蒋正生.对经络实质的理论探讨[J].针灸临床杂志.1997,13(6):15-17.
[55]张锡钧.“经络,皮层,内脏相关”假说.上海中医学院编.针灸学,人民卫生出版社,北京,1974,100.
[56]杨志永,张铁红.经络实质的假说及临床验证[J].中国针灸,188.
[57]李树德,高渌汶.经络理论新探[J].中国针灸,188.
[58]罗汀,郭义,王秀云.经络循行生化研究近况[J].上海针灸杂志,2006,25(6):45-47.
[59]郭义,张艳军,苗文方,等.钙离子是经络活动的关键因素之一[J].中国中医基础医学杂志,1998,4(7):49.
[60]郭义.家兔经穴钙离子浓度特异性的研究[J].针刺研究,1991,16(1):66.
[61]郭义.络合足三里穴 Ca2+对针刺足三里抑制正常家兔胃运动影响的实验研究[J].针刺研究,1998,23(2):146.
[62]章汉平.经络深层组织pH环境特点探讨[C].第七届全国经络学术会议论文选编,1993.
[63]成柏华,李忠华,杨红英.针刺不同经络对穴位内K~+、Na~+、Ca2+、H~+的动态变化观察[J].上海针灸杂志,1994,13(2):80-81.
[64]黄改娣.针刺镇痛与局部pH值关系的研究[C].中国针灸学会实验研究会第五次学术交流会论文摘要选编,1990,104.
[65]郭义,张艳军,苗文方,等.实验性心律失常家兔内关穴H~+ 浓度的变化的动态监测研究[J].中国中医药科技,1994,1(6):12.
[66]王秀云,郭义,张艳军,等.针刺对家兔腧穴K~+浓度的影响[J].中国中医基础医学杂志,1998,4(1):48.
[67]刘万成,孔早鸣,张晓彬.针刺家兔“阴市”穴对“伏兔”穴区肌肉组织钾、钠、钙离子含量的影响[J].黑龙江中医药,1986,2(2):33.
[68]谢远军,胡翔龙,吴宝华.经脉循行线上组织氧分压的观察及其针刺的影响[J].中国中医药科技,2000,7(6):353.
[69]费伦,承焕生,蔡德亨,等.经络物质基础及其功能性特征的实验探索和研究展望[J].科学通报,1998,43(6):866.
[70]上海经络研究所.针刺可改变穴位组织的锌含量[J].上海中医情报,1986,(3):4.
[71]邵正一,凌祥.“内关”、“足三里”注射胰岛素后对健康家兔部分外周脉线及穴位微量元素的影响[J].中国针灸,1996,16(6):37.
[78]徐淑云.药理实验方法学.第2版,北京:人民卫生出版社,1994.
[79]华兴邦.大鼠穴位图谱的研究[M].实验动物与动物实验.1991, (1):1-5.
[80]Rosenberg CA,Mun-bryce S,Wesley M,et al.Collagenase-induced intracerebral hemorrhage in rats.Stroke,1990,21:801.
[81]刘立公,顾杰,沈雪勇等.古代文献中膀胱经及其腧穴主治的统计报告[J].上海针灸杂志,2004,23(1 2):42.43.
[82]严健民.论足太阳膀胱经在经脉学说中的历史地位[J]中国中医基础医学杂志,2003,9(11):857-859.
[83]胡芝兰.脏腑背俞穴刍议[J].浙江中医学院学报,1994,18(3):47-48.
[84]陈树林,李育良.内关、心俞与心脏相关的神经基础[J].中国针灸,1996,(11):676-678.
[85]衣华强.心俞募穴与心脏特异性联系通路的荧光双标法研究[J].针刺研究,2002,27(4):245-248.
[86]尉迟静.有关心俞穴入心路线的观察[J].北京中医杂志,1986,(4):47.
[87]陈少宗.针刺神门穴对冠心病患者左心功能的即时影响[J].针灸学报,1993,(1):20-22.
[88]孙宝彩等.针刺神门治疗室上性阵发性及窦性心动过速[J].山东中医杂志,1995,14(10):471.
[89]尹改珍,徐世芬.隋·巢元方“灸背俞治五脏中风”说考析[J].中医药学刊,2003,21,(7):1134.
[90]符文彬,谢金华.司徒铃教授运用背俞穴治病经验[J].北京中医药大学学报,1995,18,(4):52-53.
[91]盛燮荪,杭宇飞.孙思邈应用背俞穴经验浅析[J].陕西中医,2000,21(5):233.
[92]徐以增等.五脏背俞穴用于中风治疗探析[J].四川中医,1998,16,(7):50-51.
[93]郁云亚.上海针灸杂志,1993,(2):54.
[94]郑宗昌,等,中国针灸,1995,(4):18.
[95]何涛,蒙华,宋风卿.105只健康大鼠心电图分析[J].JOURNAL OF PRACTICAL ELECTROCARDIOLOGY,2006,15,(4):89-290.
[96]Yamamoto S Matsui K,Sasabe M et al.effect of an o-rally active Na~+/H~+exchange inhibitor,SMP-300 experimental angina and myocardial infarction models rats.J Cardiovase Pharmacol,2002,39(2):234-241.
[97]赵瑞等.鼠实验性心肌缺血模型的心电图动态监测及分析[R].第二届中药现代化新剂型新技术国际学术会议,2006:500-506.
[98]张晋,王晓良.内皮源性血管活性因子的研究进展[J].中国约理学通报,2006,16,(1):11.
[99]王吉云.一氧化氮的病理生理作用的新观点[J].国外医学心血管疾病分册,2004,31,(1):33.
[100]Matheis G.Role of larginine-nitric oxide pathway in myocardial rcoxy-genationinjury.Am J Physical,1992,262(2):H616.
[101]Small DL,Buchan AM.Mechanisms of Cerebral Ischcmia:Intracellular Cascades and Therapeutic Interventions.J Car-diothorac Vase Ancsth,1996,10:139.
[102]姜亚军,何家声,周君富.脑出血患者氧化抗氧化状态研究[J].铁道医学.1997,25(6):335.
[103]Beckman JS,KoppenolWH.Nitric oxide,super ox-ide,and peroxynitritE:the good,the bad,and ugly[J].Am J Phsiol-Cell Physio,1 1996,271:1424-1437.
[104]刘 欣,黄作义,王复新.大鼠脑出血周边组织NO含量和NOS活性变化及对细胞凋亡的影响[J].黑龙江医药科学,2004,27(3):1-3.
[105]Peeling,J,Yan HJ,Chen SG,et al.Protective effects of free radical inhibitors in intracerebral hemorrhage in rats[J].Brain Res,1998,795(1-2):63-70.
[106]任泽光,吴建中,尹可.高血压脑出血后脑血流及自由基变化规律及其与脑水肿的关系[J].中华神经外科杂志,1995,11(4):209-212.
[107]Dorsch NW.Hemorrhagic stroke.Intracerebral and subarachnoid hemor-rhage (J).Aust Fam Physician,1997,26(10):1145-1150.
[108]Matsuo Y,Kihara T,lkedaM,et al.Role of neutrophils in radical pro-duction during ischemia and reperfusion of the rat brain:effect of neu-trophil depletion on extracellular ascorbyl radical formation(J)· J CerebBlood FlowMetab,1995,15(6):941-947.
[109]John,W Pterson,Byung-Dulk,et al.Immunologicalreaction against the aging human subarachnoid ery-throcyte[J].J Neurosurg,1989,71:718-726.
[110]Jan Ake Lindgren,TomasHokfelt,et al.Leukotrienes in the rat central nervous system.ProeNailAeadSeiUSA,1984,81:6212-6216.
[111]A-L Hulting,et al.LeukotrieneC4 as a mediator luteinizing hormone re-lease from rat anterior Pituitary cells.Proc Natl Acad.Sci.USA,1988,82:3834.
[112]Muraguchi A,Hirano T,Tang B,et al.The essential role of B cell stimulatory factor 2(BSF-2/IL-6)for the terminal differentation of B celI.JEXP MED,1998.166:33
[113]Broish L,Rostenbaum R,Albury l,et al.Activation of neurophils by interleukin-6.Cell Immunol,1989,121:280
[114]Tarkowski E,Rosengren L,Blomstrnd C,et al.Early mtratheeal Produetion interleukin-6 Prediets the size of brain lesion in stroke.Stroke,1996.27:1553
[115]郭云等.实验性大鼠脑出血急性期血浆及脑组织中白介素6的含量[J].第四军医大学学报,2001,22(25):1409-1411.
[116]杨巧莲等.脑出血患者血清IL-6含量动态变化研究[J].卒中与神经疾病,2000,7(4):233-234.
[117]Visse R,Nagase H.Matrix metalloproteinases and tissue inhibitors of metal-loproteinases.Circ Res,2003,92:827-839.
[118]Siwik DA,Colucci WS.Regulation of matrix metalloproteinases by Cytokines and reactive oxygen/nitrogen species in the my-ocardium[J].Heart Fail Rev,2004,9(1):43.
[119]Kai H,Ikeda H,Yasukawa H et al.Peripheral blood levels of matrix metallo-proteinases-2 and-9 are elevated in patients with acute coronary syndromes.J Am Coll Cardiol,1998,32:368-372.
[120]Romanic AM,Burns-Kurtis CL,Gout B,et al.Matrix metalloproteinase expression in cardiac myocytes following myocardial infarction in the rab-bit.Life Sci,2001,68(7):799-814.
[121]Hojo Y,Ikeda U,Ueno S,et al.Expression of matrix metalloproteinase in patients with acute myocardial infarction[J].Jpn Cite J,2001,65(2):71-75.
[122]Rosenberg GA.Matrix metalloproteinases in neuroinflamation[J].Glia,2002,8(39):279-283.
[123]田力,刘海峰,滕伟禹.大鼠实验性脑出血后基质金属蛋白酶 2 和 9 的表达[J].中国动脉硬化杂志,2006,14(3):221-223.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |