灵菇菌乳Ⅰ号、Ⅱ号对高血压模型大鼠的降压作用的实验研究
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摘要
灵菇菌为西藏牧民日常用食用菌类，近年来，因其具有降压作用等特殊功效而引起人们的关注。
本课题在二肾一夹型高血压大鼠动物模型基础上，利用大鼠尾动脉脉搏特征参数建立了间接测定大鼠动脉血压的回归方程，以大鼠尾动脉脉搏波特征参数及大鼠动脉血压值为客观指标，探讨了不同工艺制备的灵菇菌乳Ⅰ号、Ⅱ号对二肾一夹型高血压大鼠的血压影响及作用机制，旨在为进一步开发具有降压作用的功能性食品灵菇菌提供有价值的理论参考。
实验结果表明：
(1) 本实验根据脉搏波周期、K值、脉搏波波图面积AT、H(1+ts／td)、g／H等脉搏波特征参数建立的间接测定大鼠动脉收缩压的回归方程的方法，符合本实验要求，是作为无创性动态观察大鼠动脉血压较为理想的实验方法。
(2) 灵菇菌乳Ⅰ号低、中、高剂量组对二肾一夹型高血压模型大鼠的血压均具有降压作用，分别降低25％、27％、33％；灵菇菌乳Ⅱ号低、中、高剂量组的血压分别降低28％、30％、35％。灵菇菌乳Ⅰ号组、Ⅱ号组与给药前相比分别有显著的降压作用(P＜0.01)，并呈现出量效关系，且灵菇菌乳Ⅱ号的降压作用高于灵菇菌乳Ⅰ号；与复方降压片组无显著差异(P＞0.05)；与模型组比较有显著差异(P＜0.01)。
Ling Gu acidophilus milk is a kind of fungi which is commonly taken by Tibet herdsmen. Recently, people pay much attention to it, just because its well-known effect on blood pressure depression.
Based on the rat model of 2-kidney-l-clip hypertension, this research established a regression equation by virtue of some parameters on rat tail artery pulse waves which measured the rat artery blood pressure indirectly. According to those parameters on rat tail artery pulse waves and the value of the rat artery blood pressure, this subject explored the impact of Ling Gu acidophilus milk I II , which was produced by different technology, on experimental model of 2-kidney-l-clip hypertension rats and their mechanisms. The purpose was to supply useful theoretical references by making a kind of functional fungi for further exploration on the acidophilus milk with blood-pressure-depression function.
(1) This experiment established the regression equation for indirectly measuring rat artery systolic pressure by virtue of pulse wave parameters ,such as wave cycle, K value, AT, H(l+ts/td), g/H, was an ideal method for observing the rat artery blood pressure dynamically without damage to animal, which have performed well in the subject.
(2) Three groups of Ling Gu acidophilus milk I with low, middle and high dosage were all functional in depressing blood pressure, with the depression effect of 25%, 27% and 33% respectively; three groups of Gu acidophilus milk II with low, middle and high dosage depressed the animal blood pressure of 28%, 30%, 35% respectively. Both Ling Gu acidophilus milk I and Ling Gu acidophilus milk II could depress the blood pressure of hypertension rats which compared without them, the effects of blood pressure depression were significantly (p<0.01) and the relationship between quantum and effect was clearly showed. Of them, the blood pressure depression effects of Ling Gu acidophilus milk II were stronger than that of I . There were no obvious differences in the groups with compound depression tablets, while compared with the model groups there were obvious differences in them (P<0.01).
本课题在二肾一夹型高血压大鼠动物模型基础上，利用大鼠尾动脉脉搏特征参数建立了间接测定大鼠动脉血压的回归方程，以大鼠尾动脉脉搏波特征参数及大鼠动脉血压值为客观指标，探讨了不同工艺制备的灵菇菌乳Ⅰ号、Ⅱ号对二肾一夹型高血压大鼠的血压影响及作用机制，旨在为进一步开发具有降压作用的功能性食品灵菇菌提供有价值的理论参考。
实验结果表明：
(1) 本实验根据脉搏波周期、K值、脉搏波波图面积AT、H(1+ts／td)、g／H等脉搏波特征参数建立的间接测定大鼠动脉收缩压的回归方程的方法，符合本实验要求，是作为无创性动态观察大鼠动脉血压较为理想的实验方法。
(2) 灵菇菌乳Ⅰ号低、中、高剂量组对二肾一夹型高血压模型大鼠的血压均具有降压作用，分别降低25％、27％、33％；灵菇菌乳Ⅱ号低、中、高剂量组的血压分别降低28％、30％、35％。灵菇菌乳Ⅰ号组、Ⅱ号组与给药前相比分别有显著的降压作用(P＜0.01)，并呈现出量效关系，且灵菇菌乳Ⅱ号的降压作用高于灵菇菌乳Ⅰ号；与复方降压片组无显著差异(P＞0.05)；与模型组比较有显著差异(P＜0.01)。
Ling Gu acidophilus milk is a kind of fungi which is commonly taken by Tibet herdsmen. Recently, people pay much attention to it, just because its well-known effect on blood pressure depression.
Based on the rat model of 2-kidney-l-clip hypertension, this research established a regression equation by virtue of some parameters on rat tail artery pulse waves which measured the rat artery blood pressure indirectly. According to those parameters on rat tail artery pulse waves and the value of the rat artery blood pressure, this subject explored the impact of Ling Gu acidophilus milk I II , which was produced by different technology, on experimental model of 2-kidney-l-clip hypertension rats and their mechanisms. The purpose was to supply useful theoretical references by making a kind of functional fungi for further exploration on the acidophilus milk with blood-pressure-depression function.
(1) This experiment established the regression equation for indirectly measuring rat artery systolic pressure by virtue of pulse wave parameters ,such as wave cycle, K value, AT, H(l+ts/td), g/H, was an ideal method for observing the rat artery blood pressure dynamically without damage to animal, which have performed well in the subject.
(2) Three groups of Ling Gu acidophilus milk I with low, middle and high dosage were all functional in depressing blood pressure, with the depression effect of 25%, 27% and 33% respectively; three groups of Gu acidophilus milk II with low, middle and high dosage depressed the animal blood pressure of 28%, 30%, 35% respectively. Both Ling Gu acidophilus milk I and Ling Gu acidophilus milk II could depress the blood pressure of hypertension rats which compared without them, the effects of blood pressure depression were significantly (p<0.01) and the relationship between quantum and effect was clearly showed. Of them, the blood pressure depression effects of Ling Gu acidophilus milk II were stronger than that of I . There were no obvious differences in the groups with compound depression tablets, while compared with the model groups there were obvious differences in them (P<0.01).
引文
1 健康时报．2002．8．29
2 杨庆尧主编．国外食用菌研究[M]．上海．1983，196～198
3 杨庆尧主编．国外食用菌研究[M]．上海．1983，243～245
4 大鹤胜．投与高血压自然凳症血压及体重影响[J]．日本食品科学工学会志．1999，46(12)，806～814
5 张惠兰．植物降压成分和有降压作用的植物药[J]．中草药．1985，16(1)：38～45
6 陈芷芳．1980～1985年我国降压中草药研究概况[J]．药学通报．1987，22(2)：94～97
7 陈发春．天然降血压化合物的研究进展[J]．中草药．1991，22(7)：326～330
8 海丽娜等．天然抗高血压活性成分研究进展．云南师范大学学报．2002(22)4：74～78
9 牛天贵等．食用菌消费指南[M]．北京：农村读物出版，2000
10 黄年来．中国大型真菌原色图鉴[M]．北京：中国农业出版社，1997
11 白昆燕．食用菌资源的价值及发展潜力．衡水师专学报．2001，3(1)：54～56
12 吕作舟．食用菌生产技术手册[M]．北京：农业出版社．1997，5
13 江苏新医学院编中药大辞典[M]．上海：上海科学技术出版社．1975
14 Kino K et al.J.Biol.Chem.1989, 264: 472～476
15 马礼金，姚汝华．灵芝的药用及食用研究[J] 食品与酵工业，1998，(1)：62～63
16 刘耕陶．赤芝孢子粉的药理与临床应用[J]．中国药理通讯，1992，9(3)：4
17 曹效梅．平菇饮料的配方及工艺研究[J]．食用菌，2002(1)：38
18 李晓玉译．香菇多糖对人的作用机理．国外医学——药学分册．1985，12(3)188～189
19 吴锦文．天然生理学性物质香菇多糖和灵芝多糖的国内外研究进展．江苏食用菌．1995，16(5)：33～34
20 张松、徐章丽．多糖类医药生物活性研究进展．中国生化药物杂志．1996，17(6)：227～275
21 G MICHAELIDOU A, ALICHANIDIS E. Isolation and identification of some major water-soluble peptides in feta cheese[J].J Dairy Sci, 1998, 81: 3 109-3 116
22 张伟程等．具有ACE抑制活性乳酸菌的特性研究．中国乳品工业．2003，31(2)：9～11
23 黄哲生．人口老化与老年血压病．中国民族学院学报(自然科学版)．1996，01：76～78
24 杨医亚．中国医学史．河北科学技术出版社，1996
25 徐承涛．血管活动的调节．韩启德、文允镒主编．血管生物学．北京医科大学、协和医科大学联合出版社．1997，108～119
26 Vishwanath R. Lingappa, Krista Farey. Physiological medicine. McGraw-Hill Companies, inc. 2000
27 陈芝村．动脉血压调节机理及测量．北京人民军医出版社．1987
28 齐颂扬．医学仪器(上)高等教育出版社．1990
29 任朝晖，赵富强．现代血压测量．医疗设备信息．2001，1：32～34
30 何铁春等．四肢血压同步测量仪研制原理与医学实践．中国生物医学工程学报．2002，(21)2：182～186
31 Spainski A. Standard algorithm of blood_pressure measure measurement by oscillometeric method. Med. & Comput. 1993, 31: 204
32 Spainski A. Standard algorithm of blood_pressure measure measurement by oscillometeric method. Med. & Comput. 1992, (3): 671
33 吕书江．振动法无创血压测量原理．世界医疗器械．1999，5(11)：64～66
34 蔡国方等．自动血压监护仪．中国医疗器械杂志．1999．5(4)：15～20
35 甄斌等．示波法血压测量的实现与讨论．生物医学工程学杂志．1999，16(1)：42～45
36 江国泰、蒋大宗．非创伤性血压测量的理想化力学模型及其参数识别．中国生物医学工程学报．1991，10 (3)：167～173
37 Xu Huai—xiang, Fang Xing-ye. The Present status and Development of Oscillometric Blood Pressure Measurement. Space medicine & medical Engineering, 2000, 13(3)231～234
38 Geddes LA. The direct and indirect measurement of blood pressure. Chiacgo: year book medical publicshers. 1970: 82～130
39 Mauro ursino, Cristina Cristalli. A mathematical study of some biomechanical factors affecting the oscillometric blood.pressure measurement[J].IEEE Trans Biomed Eng, 1996, 43
40 K.Yanakoshi et al. Indirect Measurement of instantaneous Arterial Blood Pressure in the Human Finger by the Vascular Unloading Technique. IEEE Transa on Biom Eng Comput.1979, 17(1): 11～47
41 黄力宇等．基于单片机的血压监护仪的研制．中国医疗器械杂志．2002，26(4)：253～255
42 Recommandations of Human Blood Pressure Determination by Sphygmomanometers, 1987 copyright American Heart Assosiation
43 O. Brien E T, O. Malley, K.A B C of bood pressure measureee ment[J].Br Med J 1979, 2: 1201
44 焦学军、房兴业．利用脉搏波特征参数连续测量血压的方法研究．生物医学工程学杂志，2002，19 (2)：217～220
45 Flaherty et al. Rapid non-invaslve blood pressure measuring device. United States Patent, 2002
46 Shinji Kondo, et al. Pulse wave measuring apparatus and pulse wave measuring method. United
States Patent，2002
47 邹峰．脉搏波信号处理的一种简单可靠的实时算法．生物医学工程学杂志．1994，(11)2：182～183
48 De Mey C et al. Method specificity of non-invasive blood pressure measurement: oscillometry and finger pulse pressure ve, accostic methods. Br J Clin Pharmacol. 1995, 40: 291～297
49 Callaghan FJ, Geddes LA, Babbs CF, Bourland JD. Relatonship between pulse wave velocity and arterial clasticity. Med. Biol. Eng. Comput. 1986, 224: 248～254
50 Gribbin B, Steptoe A, Sleight P. Pulse wave velocity as a measuree of blood pressure change. Psychophysiol. 1976: 86～90
51 Pruett JD, Bourland JD, Geddes LA.Measurement of pulse wave velocity using a beat-sampling technique.Ann. Biomed. Eng.16: 341～347
52 Shinji Kondo, et al. Pulse wave measureing apparatus and pulse wave measuring method. United States Patent. 2002/0095092
53 Yunquan Chen, et al. Continuous non-invasive blood pressure monitoring method and apparatus. United States Patent. 2002/0058876 A1
54 Ryoichi Ochiai M.D. et al. Detetion of cardiovascular instability by pulse wave transit time. Anesth, 91(3A), 1999
55 Brian Gribbin. Pulse wave velocity as a measure of pressure change. Psychophysiology, 1976, 13(5): 87
56 Andrew Steptoe. Pulse wave velocity and blood pressure change: calibration and applications. Psychophysiology, 1976, 13 (5): 489
57 Tanaka H, Sakamoto K, Kanai H. Indirect blood pressure measurement by the pulse wave velocity method. Jap J Med Electronics Biol Eng, 1984, 22(1): 13
58 Andrew Steptoe. Pulse wave velocity and bood pressure change: calibration and application. Psychophysiology, 1976, 13 (5): 488
59 Gedes LA, Voelz M H, Babbs CF, et al. Pulse transit time as an indicator blood pressure.Psychophysiology, 1981, 18: 21
60 Brian Gribbin. Pulse wave velocity as a measure of blood pressure change. Psychophysiology, 1976, 13 (1): 86
61 焦学军，房兴业．连续每搏血压测量方法的研究进展．航天医学与医学工程．2000(13)2：148～151
62 俞梦孙．21世纪医学电子仪器．第十一届中国国际医用仪器设备展览会暨技术交流会．2002，9：9～11
63 扬子彬．当代医学新理论与新技术丛书：基础医学卷：生物医学工程学．2000
64 柳兆荣，李惜惜．血液动力学原理与方法．上海：复旦大学出版社，1997：168～196
65 Okada M. Possible determineants of pulse-wave velocity in vivo, IEEE Trans. BME. 1988, 35(5): 357
66 Shalak R, Keller SR, Secomb TW. Mechanics of blood flow, ASME Trans. Biomech. Eng, 1981: 102
67 杨天权，张镜人，柳兆荣．脉搏波的传播与临床脉诊．国外医学——生物医学工程分册．1986，8(2)：82
68 Brian Gribbin. Pulse wave velocity as a measure of blood pressure change. Psychophysiology, 1976, 13 (1): 87～90
69 Dobrin PD, Rovick AA. Influence of vascular smooth muscle on contractile mechanics and elasticity of arteries. Amer. J. Physiol. 1969, 217: 1644～1651
70 Fung YB. Mechanicai propertyes of blood vessels. In Johnson PC, ed. Peripheral circulation. J. Wiley and Sons. 1978, 45～46
71 陆渭明等．对利用脉搏波传播速度间接测量血压时主要影响因素的研究．中国生物医学工程学报．1995，14(1)：88～93
72 胡章和，李震、田孝坤．孕妇血压与其脉搏波的相关性研究与应用．生物医学工程学杂志．1995，12(2)：162～164
73 张镜人，杨天权，郑秀春．脉搏波的线化理论对正常人脑动脉脉图和脉象的分析．中国生物医学工程学报．1987，6(3)：168～173
74 柳兆荣，李惜惜．桡动脉压力波随生理参数的变化．力学学报．1982(3)：244～250
75 Chun T Lee, Ling Y Wei. Spectrum Analysis of Human Pulse. IEEE Trans. Biomed. Eng, 1983, 30(6): 348～351
76 罗志昌等．脉搏波波形特征信息的研究．北京工业大学学报．1996，22(1)：71～79
77 孔谙等．心血管系统参数变化对脉搏波波形影响的数字仿真研究．航天医学与医学工程．1999，12(4)：291～292
78 Jaron D, Moove T W, Chu C et al. A cardiovascular model for studying impairment of cerebral function during Gz stress[J].Avia Space Environ Med, 1984, 55(1): 24～31
79 王炳和，杨颙，相敬林．一种无创测定桡动脉脉搏波速度的方法及影响脉搏波速度的因素．生物医学工程学杂志 2000，17(2)：179～182
80 焦学军，房兴业．利用脉搏波特征参数连续测量血压的方法研究．生物医学工程学杂志．2002，19(2)：217～220
81 张炜芳，焦向英，刘慧荣．在体大鼠左心室内压测定方法的评价．山西医科大学学报．2000，31 (3)：193～194
82 邓原成，王新．脉象图的参数模型及其应用研究．北京生物医学工程．1994，13(1)：1
83 施新猷等．现代医学实验动物学．人民军医出版社．2000，9
84 任晓明等．实验动物技术．北京农业大学出版社．1994，9
85 孙敬方，动物实验方法学，人民卫生出版社．2001，11
86 李厚达．实验动物学．中国农业出版社．2002．7
87 石岩、梅世昌．医学动物实验使用手册．中国农业出版社．2002．12
88 尹时华，金延安，化长林．建立肾性高血压大鼠实验模型的一种新方法．咸宁医院学学报．1999，13 (1)：15～17
89 曾进胜，黄如训．易卒中肾血管高血压大鼠模型及其应用．中山医科大学学报．1996，17(4)：241～244
90 Capasso J M, Strobeck J E, Malhotra A. Contractile behavior of rat myocardium after reversal of hypertensive hypertension. Am J Physiol, 1982, 242: 882～889
91 叶惠生、李伊娜．卡托普利并钙剂治疗25例老年3级高血压的疗效观察．心血管康复医学杂志．2000，9 (3)：51～52
92 张继平、李钟文、郭振球．潜熄宁对高血压模型大鼠的降压实验及发病过程的预防作用．中药新药与临床药理．1994，5(3)：21～25
93 赵富强等．对波动法血压无创自动测量技术原理及测量准确性的实验分析．中国医学物理学杂志．1997，14(3)：182～184
94 Zheng HZ, Dong ZH, She J. Modern Study and Application of Traditional Chinese Medicine(中药现代研究与应用)[M].Vol. Beijing: Xueyuan Press, 2000
95 Facdchinettif, Longom, Piccininif, L-Arginine infusion reduces blood pressure in preeclamptic women through nitric oxide release[J] Soc Gyneoolinaest, 1999, 6(4): 202～207
96 王梅，沈辉．食物蛋白酶解物中的生物活性肽[J]．氨基酸与生物资源．1997，19(1)：40～43
97 Moeller I. AM.J Hum Hypertens. 1998 May: 12(5): 289～293
98 郑建仙．功能性食品[M]．中国轻工业出版社．1995，347～352
99 茂原，杉朴友美．今年GABA生理机能研究——脑机能改善作用．食品开发．2001，36(6)
100 万选才，杨天祝，徐承焘．现代神经生物学．北京医科大学中国协和医科大学联合出版社．1999，162～185
101 Okada T, Sugishila T, Murakami T, et al. Ettecl of defalled rice germ enriched with GABA for sleeplessness, depression, autonomie disorder by oral administration. Nippon Shokuhin Kagaku Kashi. 2000, 47(8): 596～603
102 许建军，江波，许时婴．生物合成γ-氨基丁酸的乳酸菌的筛选．食品科技．2002，10：7～10
103 田庚元等．多糖类免疫调节剂的研究和应用．化学进展．1994，6(2)：114～124
104 Miyakoshi et al. Int. J. Immunopharmacol. 1989, 11, 365
105 Fenichel, R.L. et al. Immune Modulation Agents and Their Mechanism, New yourk. Marcel Dekker Inc.1984, 409
106 友田正司，现代东洋医学，1989，10，91
107 Francis SH, Corbin JD. Structure and function of cyclic nucleotide-dependenr protein kinase[J].Annu Rev Physiol, 1994, 56: 237
108 Rai JL. Nitric oxide and kidney[J]. Circulation. 1993, 87: 26
109 Garcia NH, Stoos BA, Carretero OA, et al. Mechanism of the nitric oxide induced blockade of collecting duct water permeability[J].Hypertens, 1996, 270: 794
110 Granger J, Novak J, Schnackenberg C, et al. Role of renal nerves in mediating the hypertensive effects of nitric oxids synthesis inhibition[J]. Hypertens, 1996, 27(3): 613
111 Ferro CJ, Webb DJ. Endorhelial dysfunction and hypetrtension[J].Drugs, 1997, 53(1): 30
2 杨庆尧主编．国外食用菌研究[M]．上海．1983，196～198
3 杨庆尧主编．国外食用菌研究[M]．上海．1983，243～245
4 大鹤胜．投与高血压自然凳症血压及体重影响[J]．日本食品科学工学会志．1999，46(12)，806～814
5 张惠兰．植物降压成分和有降压作用的植物药[J]．中草药．1985，16(1)：38～45
6 陈芷芳．1980～1985年我国降压中草药研究概况[J]．药学通报．1987，22(2)：94～97
7 陈发春．天然降血压化合物的研究进展[J]．中草药．1991，22(7)：326～330
8 海丽娜等．天然抗高血压活性成分研究进展．云南师范大学学报．2002(22)4：74～78
9 牛天贵等．食用菌消费指南[M]．北京：农村读物出版，2000
10 黄年来．中国大型真菌原色图鉴[M]．北京：中国农业出版社，1997
11 白昆燕．食用菌资源的价值及发展潜力．衡水师专学报．2001，3(1)：54～56
12 吕作舟．食用菌生产技术手册[M]．北京：农业出版社．1997，5
13 江苏新医学院编中药大辞典[M]．上海：上海科学技术出版社．1975
14 Kino K et al.J.Biol.Chem.1989, 264: 472～476
15 马礼金，姚汝华．灵芝的药用及食用研究[J] 食品与酵工业，1998，(1)：62～63
16 刘耕陶．赤芝孢子粉的药理与临床应用[J]．中国药理通讯，1992，9(3)：4
17 曹效梅．平菇饮料的配方及工艺研究[J]．食用菌，2002(1)：38
18 李晓玉译．香菇多糖对人的作用机理．国外医学——药学分册．1985，12(3)188～189
19 吴锦文．天然生理学性物质香菇多糖和灵芝多糖的国内外研究进展．江苏食用菌．1995，16(5)：33～34
20 张松、徐章丽．多糖类医药生物活性研究进展．中国生化药物杂志．1996，17(6)：227～275
21 G MICHAELIDOU A, ALICHANIDIS E. Isolation and identification of some major water-soluble peptides in feta cheese[J].J Dairy Sci, 1998, 81: 3 109-3 116
22 张伟程等．具有ACE抑制活性乳酸菌的特性研究．中国乳品工业．2003，31(2)：9～11
23 黄哲生．人口老化与老年血压病．中国民族学院学报(自然科学版)．1996，01：76～78
24 杨医亚．中国医学史．河北科学技术出版社，1996
25 徐承涛．血管活动的调节．韩启德、文允镒主编．血管生物学．北京医科大学、协和医科大学联合出版社．1997，108～119
26 Vishwanath R. Lingappa, Krista Farey. Physiological medicine. McGraw-Hill Companies, inc. 2000
27 陈芝村．动脉血压调节机理及测量．北京人民军医出版社．1987
28 齐颂扬．医学仪器(上)高等教育出版社．1990
29 任朝晖，赵富强．现代血压测量．医疗设备信息．2001，1：32～34
30 何铁春等．四肢血压同步测量仪研制原理与医学实践．中国生物医学工程学报．2002，(21)2：182～186
31 Spainski A. Standard algorithm of blood_pressure measure measurement by oscillometeric method. Med. & Comput. 1993, 31: 204
32 Spainski A. Standard algorithm of blood_pressure measure measurement by oscillometeric method. Med. & Comput. 1992, (3): 671
33 吕书江．振动法无创血压测量原理．世界医疗器械．1999，5(11)：64～66
34 蔡国方等．自动血压监护仪．中国医疗器械杂志．1999．5(4)：15～20
35 甄斌等．示波法血压测量的实现与讨论．生物医学工程学杂志．1999，16(1)：42～45
36 江国泰、蒋大宗．非创伤性血压测量的理想化力学模型及其参数识别．中国生物医学工程学报．1991，10 (3)：167～173
37 Xu Huai—xiang, Fang Xing-ye. The Present status and Development of Oscillometric Blood Pressure Measurement. Space medicine & medical Engineering, 2000, 13(3)231～234
38 Geddes LA. The direct and indirect measurement of blood pressure. Chiacgo: year book medical publicshers. 1970: 82～130
39 Mauro ursino, Cristina Cristalli. A mathematical study of some biomechanical factors affecting the oscillometric blood.pressure measurement[J].IEEE Trans Biomed Eng, 1996, 43
40 K.Yanakoshi et al. Indirect Measurement of instantaneous Arterial Blood Pressure in the Human Finger by the Vascular Unloading Technique. IEEE Transa on Biom Eng Comput.1979, 17(1): 11～47
41 黄力宇等．基于单片机的血压监护仪的研制．中国医疗器械杂志．2002，26(4)：253～255
42 Recommandations of Human Blood Pressure Determination by Sphygmomanometers, 1987 copyright American Heart Assosiation
43 O. Brien E T, O. Malley, K.A B C of bood pressure measureee ment[J].Br Med J 1979, 2: 1201
44 焦学军、房兴业．利用脉搏波特征参数连续测量血压的方法研究．生物医学工程学杂志，2002，19 (2)：217～220
45 Flaherty et al. Rapid non-invaslve blood pressure measuring device. United States Patent, 2002
46 Shinji Kondo, et al. Pulse wave measuring apparatus and pulse wave measuring method. United
States Patent，2002
47 邹峰．脉搏波信号处理的一种简单可靠的实时算法．生物医学工程学杂志．1994，(11)2：182～183
48 De Mey C et al. Method specificity of non-invasive blood pressure measurement: oscillometry and finger pulse pressure ve, accostic methods. Br J Clin Pharmacol. 1995, 40: 291～297
49 Callaghan FJ, Geddes LA, Babbs CF, Bourland JD. Relatonship between pulse wave velocity and arterial clasticity. Med. Biol. Eng. Comput. 1986, 224: 248～254
50 Gribbin B, Steptoe A, Sleight P. Pulse wave velocity as a measuree of blood pressure change. Psychophysiol. 1976: 86～90
51 Pruett JD, Bourland JD, Geddes LA.Measurement of pulse wave velocity using a beat-sampling technique.Ann. Biomed. Eng.16: 341～347
52 Shinji Kondo, et al. Pulse wave measureing apparatus and pulse wave measuring method. United States Patent. 2002/0095092
53 Yunquan Chen, et al. Continuous non-invasive blood pressure monitoring method and apparatus. United States Patent. 2002/0058876 A1
54 Ryoichi Ochiai M.D. et al. Detetion of cardiovascular instability by pulse wave transit time. Anesth, 91(3A), 1999
55 Brian Gribbin. Pulse wave velocity as a measure of pressure change. Psychophysiology, 1976, 13(5): 87
56 Andrew Steptoe. Pulse wave velocity and blood pressure change: calibration and applications. Psychophysiology, 1976, 13 (5): 489
57 Tanaka H, Sakamoto K, Kanai H. Indirect blood pressure measurement by the pulse wave velocity method. Jap J Med Electronics Biol Eng, 1984, 22(1): 13
58 Andrew Steptoe. Pulse wave velocity and bood pressure change: calibration and application. Psychophysiology, 1976, 13 (5): 488
59 Gedes LA, Voelz M H, Babbs CF, et al. Pulse transit time as an indicator blood pressure.Psychophysiology, 1981, 18: 21
60 Brian Gribbin. Pulse wave velocity as a measure of blood pressure change. Psychophysiology, 1976, 13 (1): 86
61 焦学军，房兴业．连续每搏血压测量方法的研究进展．航天医学与医学工程．2000(13)2：148～151
62 俞梦孙．21世纪医学电子仪器．第十一届中国国际医用仪器设备展览会暨技术交流会．2002，9：9～11
63 扬子彬．当代医学新理论与新技术丛书：基础医学卷：生物医学工程学．2000
64 柳兆荣，李惜惜．血液动力学原理与方法．上海：复旦大学出版社，1997：168～196
65 Okada M. Possible determineants of pulse-wave velocity in vivo, IEEE Trans. BME. 1988, 35(5): 357
66 Shalak R, Keller SR, Secomb TW. Mechanics of blood flow, ASME Trans. Biomech. Eng, 1981: 102
67 杨天权，张镜人，柳兆荣．脉搏波的传播与临床脉诊．国外医学——生物医学工程分册．1986，8(2)：82
68 Brian Gribbin. Pulse wave velocity as a measure of blood pressure change. Psychophysiology, 1976, 13 (1): 87～90
69 Dobrin PD, Rovick AA. Influence of vascular smooth muscle on contractile mechanics and elasticity of arteries. Amer. J. Physiol. 1969, 217: 1644～1651
70 Fung YB. Mechanicai propertyes of blood vessels. In Johnson PC, ed. Peripheral circulation. J. Wiley and Sons. 1978, 45～46
71 陆渭明等．对利用脉搏波传播速度间接测量血压时主要影响因素的研究．中国生物医学工程学报．1995，14(1)：88～93
72 胡章和，李震、田孝坤．孕妇血压与其脉搏波的相关性研究与应用．生物医学工程学杂志．1995，12(2)：162～164
73 张镜人，杨天权，郑秀春．脉搏波的线化理论对正常人脑动脉脉图和脉象的分析．中国生物医学工程学报．1987，6(3)：168～173
74 柳兆荣，李惜惜．桡动脉压力波随生理参数的变化．力学学报．1982(3)：244～250
75 Chun T Lee, Ling Y Wei. Spectrum Analysis of Human Pulse. IEEE Trans. Biomed. Eng, 1983, 30(6): 348～351
76 罗志昌等．脉搏波波形特征信息的研究．北京工业大学学报．1996，22(1)：71～79
77 孔谙等．心血管系统参数变化对脉搏波波形影响的数字仿真研究．航天医学与医学工程．1999，12(4)：291～292
78 Jaron D, Moove T W, Chu C et al. A cardiovascular model for studying impairment of cerebral function during Gz stress[J].Avia Space Environ Med, 1984, 55(1): 24～31
79 王炳和，杨颙，相敬林．一种无创测定桡动脉脉搏波速度的方法及影响脉搏波速度的因素．生物医学工程学杂志 2000，17(2)：179～182
80 焦学军，房兴业．利用脉搏波特征参数连续测量血压的方法研究．生物医学工程学杂志．2002，19(2)：217～220
81 张炜芳，焦向英，刘慧荣．在体大鼠左心室内压测定方法的评价．山西医科大学学报．2000，31 (3)：193～194
82 邓原成，王新．脉象图的参数模型及其应用研究．北京生物医学工程．1994，13(1)：1
83 施新猷等．现代医学实验动物学．人民军医出版社．2000，9
84 任晓明等．实验动物技术．北京农业大学出版社．1994，9
85 孙敬方，动物实验方法学，人民卫生出版社．2001，11
86 李厚达．实验动物学．中国农业出版社．2002．7
87 石岩、梅世昌．医学动物实验使用手册．中国农业出版社．2002．12
88 尹时华，金延安，化长林．建立肾性高血压大鼠实验模型的一种新方法．咸宁医院学学报．1999，13 (1)：15～17
89 曾进胜，黄如训．易卒中肾血管高血压大鼠模型及其应用．中山医科大学学报．1996，17(4)：241～244
90 Capasso J M, Strobeck J E, Malhotra A. Contractile behavior of rat myocardium after reversal of hypertensive hypertension. Am J Physiol, 1982, 242: 882～889
91 叶惠生、李伊娜．卡托普利并钙剂治疗25例老年3级高血压的疗效观察．心血管康复医学杂志．2000，9 (3)：51～52
92 张继平、李钟文、郭振球．潜熄宁对高血压模型大鼠的降压实验及发病过程的预防作用．中药新药与临床药理．1994，5(3)：21～25
93 赵富强等．对波动法血压无创自动测量技术原理及测量准确性的实验分析．中国医学物理学杂志．1997，14(3)：182～184
94 Zheng HZ, Dong ZH, She J. Modern Study and Application of Traditional Chinese Medicine(中药现代研究与应用)[M].Vol. Beijing: Xueyuan Press, 2000
95 Facdchinettif, Longom, Piccininif, L-Arginine infusion reduces blood pressure in preeclamptic women through nitric oxide release[J] Soc Gyneoolinaest, 1999, 6(4): 202～207
96 王梅，沈辉．食物蛋白酶解物中的生物活性肽[J]．氨基酸与生物资源．1997，19(1)：40～43
97 Moeller I. AM.J Hum Hypertens. 1998 May: 12(5): 289～293
98 郑建仙．功能性食品[M]．中国轻工业出版社．1995，347～352
99 茂原，杉朴友美．今年GABA生理机能研究——脑机能改善作用．食品开发．2001，36(6)
100 万选才，杨天祝，徐承焘．现代神经生物学．北京医科大学中国协和医科大学联合出版社．1999，162～185
101 Okada T, Sugishila T, Murakami T, et al. Ettecl of defalled rice germ enriched with GABA for sleeplessness, depression, autonomie disorder by oral administration. Nippon Shokuhin Kagaku Kashi. 2000, 47(8): 596～603
102 许建军，江波，许时婴．生物合成γ-氨基丁酸的乳酸菌的筛选．食品科技．2002，10：7～10
103 田庚元等．多糖类免疫调节剂的研究和应用．化学进展．1994，6(2)：114～124
104 Miyakoshi et al. Int. J. Immunopharmacol. 1989, 11, 365
105 Fenichel, R.L. et al. Immune Modulation Agents and Their Mechanism, New yourk. Marcel Dekker Inc.1984, 409
106 友田正司，现代东洋医学，1989，10，91
107 Francis SH, Corbin JD. Structure and function of cyclic nucleotide-dependenr protein kinase[J].Annu Rev Physiol, 1994, 56: 237
108 Rai JL. Nitric oxide and kidney[J]. Circulation. 1993, 87: 26
109 Garcia NH, Stoos BA, Carretero OA, et al. Mechanism of the nitric oxide induced blockade of collecting duct water permeability[J].Hypertens, 1996, 270: 794
110 Granger J, Novak J, Schnackenberg C, et al. Role of renal nerves in mediating the hypertensive effects of nitric oxids synthesis inhibition[J]. Hypertens, 1996, 27(3): 613
111 Ferro CJ, Webb DJ. Endorhelial dysfunction and hypetrtension[J].Drugs, 1997, 53(1): 30