纳米党参合剂调整肠道微生态失衡防治多器官功能障碍综合症的研究
摘要
目的:本实验研究了纳米党参合剂通过调整菌群失调,修复肠屏障功能,控制细菌、内毒素易位,对多器官功能障碍大鼠肠源性感染及内毒素血症(IETM)进行防治,减轻由IETM及其引起的细胞因子连锁反应造成对多器官功能障碍(MODS)的“二次打击”,达到改善相应症状,降低死亡率的目的。
方法:选用Wistar大鼠70只,采用肠缺血再灌注的方法制造MODS的模型。分别以纳米中药、常态中药、丽珠肠乐对动物进行灌胃治疗,一周后处死动物。取肠内容物、肝、肺、脾、肠、肠系膜淋巴结,门静脉血,分别做无菌培养,病理学切片,CD4+、CD4+/CD8+计数,内毒素及SIgA的测定。
结果:本实验成功的复制出多器官功能障碍的模型,模型建立组动物出现菌群失调,细菌易位,内毒素血症;同时伴有两个及两个以上脏器损伤;CD4+减少,CD4+/CD8+比值下降甚至出现比值倒置,SIgA水平明显下降。与正常对照组相比差异显著。各治疗组通过扶植肠道有益菌、抑制有害菌的生长繁殖并促进其排泄,保护肠黏膜屏障,明显降低了细菌易位,控制内毒素血症,改善了其他各相关病理、免疫指标。整体疗效,纳米中药、常态中药及丽珠肠乐均有效,但纳米中药在控制内毒素血症,提高免疫力等方面优于常态中药和丽珠肠乐,差异有统计学意义。
结论:肠缺血再灌注造成的MODS伴有严重菌群失调,细菌、内毒素易位,是MODS发生、发展的重要因素。党参合剂从调整微生态失调的角度来防治MODS取得较好效果,纳米中药效果更佳。
Objective: We designed the experiment in order to study Nano- dangshen mixture prevent and cure intestinal infection and endotoxemia (IETM), by adjusting dysbacteriosis, repair intestinal barrier function, control bacteria and toxins translocation, in the Multiple Organ Dysfunction Syndrome (MODS).Abating“The Secondary Injury”to MODS, caused by IETM and cell factor chain reaction, to improve the corresponding symptom, and reduce mortality.
Method: Selection of 70 Wistar rats, to prepared the rat model of MODS by intestinal ischemia- reperfusion. Intragastric administration with Nano Chinese medicine, Normal Chinese medicine and Bifidobiogen Livzon respectively.Then after one week the animals were killed to detected the changing of bacteria quantity and location, determination of plasma endo- toxin level, intestinal contents level of SIgA, CD4 +, CD8 + counting, and pathology examination.
Results: This study to reproduced MODS model successfully, The model group had dysbacteriosis, bacterial and endotoxin translocation, at the same time with two and two or more organ damaged, CD4 + reduction, CD4 + / CD8 + ratio fell even inverted ratio, SIgA level decreased significantly. Compared with the normal group significantly. The therapy groups reduced bacterial and endotoxin translocation significantly, controlled IETM , improved relevance index of pathology and immunity, by regulating intestinal dysbacteriosis, and repairing intestine barrier function.The overall effect, Nano-CM, Normal-CM and Bifidobiogen Livzon were all effective, but Nano-CM was better than Normal-CM, and Bifidobiogen Livzon in the effect of controlling IETM and improving immunity, the difference was significant(p<0.05).
Conclusion: The model group has serious dysbacteriosis, bacteria and toxins translocation which is the key factors of MODS occurrence and development. Dangshen mixture prevent MODS by regulating intestinal dysbacteriosis get fairly good effect, by comparing with Normal-CM, Nano-CM, we concluded that the later is more effevtive than the former .
方法:选用Wistar大鼠70只,采用肠缺血再灌注的方法制造MODS的模型。分别以纳米中药、常态中药、丽珠肠乐对动物进行灌胃治疗,一周后处死动物。取肠内容物、肝、肺、脾、肠、肠系膜淋巴结,门静脉血,分别做无菌培养,病理学切片,CD4+、CD4+/CD8+计数,内毒素及SIgA的测定。
结果:本实验成功的复制出多器官功能障碍的模型,模型建立组动物出现菌群失调,细菌易位,内毒素血症;同时伴有两个及两个以上脏器损伤;CD4+减少,CD4+/CD8+比值下降甚至出现比值倒置,SIgA水平明显下降。与正常对照组相比差异显著。各治疗组通过扶植肠道有益菌、抑制有害菌的生长繁殖并促进其排泄,保护肠黏膜屏障,明显降低了细菌易位,控制内毒素血症,改善了其他各相关病理、免疫指标。整体疗效,纳米中药、常态中药及丽珠肠乐均有效,但纳米中药在控制内毒素血症,提高免疫力等方面优于常态中药和丽珠肠乐,差异有统计学意义。
结论:肠缺血再灌注造成的MODS伴有严重菌群失调,细菌、内毒素易位,是MODS发生、发展的重要因素。党参合剂从调整微生态失调的角度来防治MODS取得较好效果,纳米中药效果更佳。
Objective: We designed the experiment in order to study Nano- dangshen mixture prevent and cure intestinal infection and endotoxemia (IETM), by adjusting dysbacteriosis, repair intestinal barrier function, control bacteria and toxins translocation, in the Multiple Organ Dysfunction Syndrome (MODS).Abating“The Secondary Injury”to MODS, caused by IETM and cell factor chain reaction, to improve the corresponding symptom, and reduce mortality.
Method: Selection of 70 Wistar rats, to prepared the rat model of MODS by intestinal ischemia- reperfusion. Intragastric administration with Nano Chinese medicine, Normal Chinese medicine and Bifidobiogen Livzon respectively.Then after one week the animals were killed to detected the changing of bacteria quantity and location, determination of plasma endo- toxin level, intestinal contents level of SIgA, CD4 +, CD8 + counting, and pathology examination.
Results: This study to reproduced MODS model successfully, The model group had dysbacteriosis, bacterial and endotoxin translocation, at the same time with two and two or more organ damaged, CD4 + reduction, CD4 + / CD8 + ratio fell even inverted ratio, SIgA level decreased significantly. Compared with the normal group significantly. The therapy groups reduced bacterial and endotoxin translocation significantly, controlled IETM , improved relevance index of pathology and immunity, by regulating intestinal dysbacteriosis, and repairing intestine barrier function.The overall effect, Nano-CM, Normal-CM and Bifidobiogen Livzon were all effective, but Nano-CM was better than Normal-CM, and Bifidobiogen Livzon in the effect of controlling IETM and improving immunity, the difference was significant(p<0.05).
Conclusion: The model group has serious dysbacteriosis, bacteria and toxins translocation which is the key factors of MODS occurrence and development. Dangshen mixture prevent MODS by regulating intestinal dysbacteriosis get fairly good effect, by comparing with Normal-CM, Nano-CM, we concluded that the later is more effevtive than the former .
引文
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