哈萨克族EH患者血浆视黄醇结合蛋白4水平及其相关性研究
摘要
目的:检测新疆哈萨克族(哈族)原发性高血压(EH)患者的血浆视黄醇结合蛋白4(RBP4)水平,比较EH组及对照组血浆RBP4水平有无差异；分析哈族EH患者血浆RBP4水平与血压、血糖、血脂、胰岛素、胰岛素抵抗(IR)及肥胖度指标的相关性。
立法:选取新疆哈族人81例作为研究对象,根据血压水平,分为哈族EH组41例、哈族对照组40例。测量血压、身高、体重、腰围(WC)及臀围。抽取空腹静脉血,测定其血浆RBP4、血糖(FPG)、总胆固醇(TC)、甘油三脂(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、载脂蛋白Al (Apo-Al)、载脂蛋白B (Apo-B)和胰岛素(FINS)。计算体重指数(BMI)、腰臀比(WHR)、体内脂肪百分比(BF%)、HOMA-胰岛素抵抗指数(HOMA-IR)。用酶联免疫吸附试验(ELISA法)测定血浆RBP4浓度,电化学发光法测定血浆FINS浓度,全自动生化分析仪测定血糖、血脂。采用t检验、相关及多元逐步回归法对数据进行处理。
结果:.哈族EH组的血浆RBP4水平明显高于相应的对照组(P<0.01)。2. Pearson相关分析提示：哈族EH组的血浆RBP4与年龄、SBP、DBP、WC、WHR、FPG、HOMA-IR呈显著正相关；在对照组中,RBP4与FPG呈正相关,与BF%成负相关。③多元回归分析显示：在EH组中,SBP和WHR进入回归方程,是影响血浆RBP4水平的独立因素；在对照组中,BF%进入回归方程,是影响血浆RBP4水平的独立因素。
结途:哈族EH患者血浆RBP4水平较对照组升高,哈族EH组血浆RBP4与血压、WC、WHR、FPG、HOMA-IR呈显著正相关,RBP4可能通过促进胰岛素抵抗而参与了EH的发生、发展。
Objective To explore the plasma retinol binding protein 4 (RBP4) levels in the patients with essential hypertension(EH) of Xinjiang Kazakans and the relations between plasma RBP4 level and blood pressure、blood lipids、blood glucose、insulin、insulin resistance (IR)、waist hip ratio (WHR) and obesity.
Methods Totally 81 Kazakans cases were selected for the subjects. They were divided into two groups according to their blood pressure:EH group(n=41)and control group(n=40). Measurment of blood pressure, body height, body mass,waist circumference(WC) and hip circumference. Fasting venous blood was drawn to detect the plasma RBP4、fasting insulin(FINS)、fasting plasma glucose(FPG)、total cholesterol(TC)、triglyceride(TG)、high-density lipoprotein cholesterol(HDL-C)、low-density lipoprotein cholesterol (LDL-C)、apoprotein-Al (Apo-Al) and apoprotein-B (Apo-B),calculating body mass index(BMI)、waist-hip ratio(WHR)、body fat percentage(BF%)and homeostasis model assessment of insulin resistance index(HOMA-IR).
Results①The plasma RBP4 concentration in EH group was significantly higher than that in control group(P<0.01).②RBP4 was positively and significantly correlated with age、systolic blood pressure(SBP)、diastolic blood pressure (DBP)、WC、WHR、FPG、HOMA-IR in EH group,and positively and significantly correlated with FPG in control group.③Multiple regression analysis showed that SBP and WHR were independent factors to affect the RBP4 level in EH group,FPG was independent factors to affect the plasma RBP4 level in control group.
Conclusions Concentration is increased in the patients with EH of Kazakans,and there is correlation between RBP4 and blood pressure、WC、WHR、FPG、HOMA-IR.RBP4 may affect blood pressure、blood glucose、WC、WHR by IR.
立法:选取新疆哈族人81例作为研究对象,根据血压水平,分为哈族EH组41例、哈族对照组40例。测量血压、身高、体重、腰围(WC)及臀围。抽取空腹静脉血,测定其血浆RBP4、血糖(FPG)、总胆固醇(TC)、甘油三脂(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、载脂蛋白Al (Apo-Al)、载脂蛋白B (Apo-B)和胰岛素(FINS)。计算体重指数(BMI)、腰臀比(WHR)、体内脂肪百分比(BF%)、HOMA-胰岛素抵抗指数(HOMA-IR)。用酶联免疫吸附试验(ELISA法)测定血浆RBP4浓度,电化学发光法测定血浆FINS浓度,全自动生化分析仪测定血糖、血脂。采用t检验、相关及多元逐步回归法对数据进行处理。
结果:.哈族EH组的血浆RBP4水平明显高于相应的对照组(P<0.01)。2. Pearson相关分析提示：哈族EH组的血浆RBP4与年龄、SBP、DBP、WC、WHR、FPG、HOMA-IR呈显著正相关；在对照组中,RBP4与FPG呈正相关,与BF%成负相关。③多元回归分析显示：在EH组中,SBP和WHR进入回归方程,是影响血浆RBP4水平的独立因素；在对照组中,BF%进入回归方程,是影响血浆RBP4水平的独立因素。
结途:哈族EH患者血浆RBP4水平较对照组升高,哈族EH组血浆RBP4与血压、WC、WHR、FPG、HOMA-IR呈显著正相关,RBP4可能通过促进胰岛素抵抗而参与了EH的发生、发展。
Objective To explore the plasma retinol binding protein 4 (RBP4) levels in the patients with essential hypertension(EH) of Xinjiang Kazakans and the relations between plasma RBP4 level and blood pressure、blood lipids、blood glucose、insulin、insulin resistance (IR)、waist hip ratio (WHR) and obesity.
Methods Totally 81 Kazakans cases were selected for the subjects. They were divided into two groups according to their blood pressure:EH group(n=41)and control group(n=40). Measurment of blood pressure, body height, body mass,waist circumference(WC) and hip circumference. Fasting venous blood was drawn to detect the plasma RBP4、fasting insulin(FINS)、fasting plasma glucose(FPG)、total cholesterol(TC)、triglyceride(TG)、high-density lipoprotein cholesterol(HDL-C)、low-density lipoprotein cholesterol (LDL-C)、apoprotein-Al (Apo-Al) and apoprotein-B (Apo-B),calculating body mass index(BMI)、waist-hip ratio(WHR)、body fat percentage(BF%)and homeostasis model assessment of insulin resistance index(HOMA-IR).
Results①The plasma RBP4 concentration in EH group was significantly higher than that in control group(P<0.01).②RBP4 was positively and significantly correlated with age、systolic blood pressure(SBP)、diastolic blood pressure (DBP)、WC、WHR、FPG、HOMA-IR in EH group,and positively and significantly correlated with FPG in control group.③Multiple regression analysis showed that SBP and WHR were independent factors to affect the RBP4 level in EH group,FPG was independent factors to affect the plasma RBP4 level in control group.
Conclusions Concentration is increased in the patients with EH of Kazakans,and there is correlation between RBP4 and blood pressure、WC、WHR、FPG、HOMA-IR.RBP4 may affect blood pressure、blood glucose、WC、WHR by IR.
引文
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