CPP-ACP对釉质早期脱矿与再矿化作用的基础和应用研究
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摘要
釉质的破坏首先从脱矿开始,先是表层脱矿,继而进入表层下,形成肉眼所见的白斑,这时如果进行治疗,仍有使脱矿釉质复原的可能性。正畸患者往往是白斑的高发人群,并且有些白斑在正畸治疗结束后数年仍持续存在,严重影响了正畸的美学效果。学者们多年来对这类白斑病损始终是束手无策,但酪蛋白磷酸肽-无定形磷酸肽(CPP-ACP)的出现,将对这种情况有所改变。CPP-ACP通过富含的磷酸丝氨酸基团,在中性和碱性条件下稳定100倍以上的钙磷离子,防止它们发生自发性沉淀。本研究在已获得的较高浓度的CPP-ACP溶液为基础,采用激光粒度仪对溶液进行检测;然后用显微放射照相、扫描电镜(SEM)、原子力显微镜(AFM)等方法评估CPP-ACP在体外和体内的抗酸性和再矿化作用,以及与氟之间的作用关系进行了探讨。
已有研究证实CPP-ACP以纳米级晶簇的形式存在,而纳米级的粒子在溶液中极易发生团聚,但关于溶液中CPP-ACP微粒的群体存在状态却没有相关的研究报道,以往研究中使用的溶液浓度均为较低的浓度(0.1%~1%),本研究将CPP浓度提高到了5%,并相应增加钙磷浓度,得到了稳定的胶体状悬浮溶液。本研究通过对CPP-ACP溶液的粒度及粒径分布的检测,发现了在水溶液中CPP-ACP纳米晶簇以团聚的状态保持平衡,并且随CPP浓度的增加,团聚的程度越小,适当的氟离子在一定程度上可以减小这种团聚。通过显微放射照相技术量化研究了CPP-ACP在体外的抗酸和再矿化效果,结果表明,5%CPP-ACP具有显著的提高釉质抗酸性的作用,与20g/L氟化钠相似;而不同浓度的CPP-ACP在体外均可引起釉质表层下脱矿病损全层的均质性再矿化,再矿化程度呈浓度依赖性;同时对表层的釉质的SEM观察,发现CPP-ACP对表层下修复的同时还将表层釉质的破坏复原了,并且新形成的矿物质与残余釉质结构有机的结合,使修复后的釉质表面结构紧密、光滑平整,类似于正常釉质;在软化釉质模型中应用AFM观察了CPP-ACP的再矿化作用,结果显示在CPP-ACP的作用下,受损釉质的纳米结构得到了修复;将CPP-ACP应用于临床正畸后白斑的治疗,图像分析结果证实CPP-ACP可以显著减小白斑的面积,经过较长时间的使用,可以完全消除白斑,提高正畸的美学效果。
Under normal circumstances, the enamel demineralization and remineralization maintain a dynamic equilibrium. The use of fixed appliance leads this equilibrium to the enamel demineralization. So the patients received the orthodontic treatment are often of high risk to enamel demineralization. Early demineralization of the enamel mostly confined to the surface layer. If the appliance was removed in time, it would impossible to be restored by saliva remineralization. However, at most of the time, when the appliance couldn’t be removed immediately, the demineralization of surface layer gradually develops to the subsurface layer, forming the visible white spots. At this time, the lesion is only the loss of the minerals of local enamel, which hasn’t caused the destructure and has the possibility of restoring the demineralized enamel. However, the remineralization capacity of saliva is very limited at this time, and needs other measures to remineralize. The key of remineralization is the supplement of losted calcium phosphate ions, but because of the low dissolubility of the calcium phosphate, it is difficult to maintain high concentration bioavailable ions at the surface of the enamel to diffuse into the subsurface enamel. So in traditional methods, calcium and phosphate could only be use at very low concentration. Methods of remineralization are also limited to the enamel surface laeyer by minerals depositing. But there’s little effect of restoration in the enamel inner lesion. Therefore, seeking the ways of restoration of the demineralization subsurface layer rather than masked by modification is always the ideal goal of the scholars.
The finding of casein phospho-peptides has brought a new breakthrough in this research field. Casein phosphopeptides (CPPs), a bioactive peptide, which is rich of phosphoserine, is obtained from milk casein by hydrolization, segregation and purification. It has the significant capacity of stablizing the calcium and phosphate ions in solution, and can combine with a big amount of calcium, phosphate and OH- ions before spontaneous precipitation under neutral or alkalinity circumstances. So in CPPs aqueous solution, the stablized calcium and phosphate is 100 times more than usual, and forming the nanocomplex, casein phosphopeptide-amorphous calcium phosphate (CPP-ACP). CPP-ACP, acts as the good vector for calcium and phosphate ions, not only supply ions, but also prevent them from spontaneous pricipitation at the enamel surface. Often it promotes the ions diffuse into the subsurface enamel in an appropriate molar ratio. The unique nature of CPP-ACP makes it have a broad application prospects in remineralization of demineralized enamel.
Some Scholars had proposed that the CPP combinated with the forming ACP, forming a metastable solution. The researches of CPP-ACP nano micro particle have also confirmed its status of non-crystal. But as the micro-particles of the nano level easily tend to gather together in the solution, there’s no relative report of domestic or alien about whether the CPP-ACP particles form together in the solution and the state of existing, as well as the effect of concentration or fluoride. In order to investigate the feature of the particle size distribution and the existant form of nanoparticles groups, we used the laser particle size analysis in experiment 1. By measuring the size and distribution of the CPP-ACP, its aggregation and stability is reflected. By increasing the concentration of 0.1%~1% in usual experiment to 5%, as the result, we got stable 5% CPP-ACP colloidal solution, and with the concentration of CPP increases, the particle size of CPP-ACP gradually decreased and the distribution became narrow. When appropriate concentration of fluoride ions added, the particel size was further reduced, showing that in solution of high concentration, the nanoclusters dispersed better, gather together less, and the size and distribution was relatively uniform. The remeasurement carried out one month later showed that the size and distribution of particle had no significantly change, which confirmed that the CPP-ACP solution in the current experiment was relatively stable which laid the foundation for the followed-up experiments.
The remineralization effect of CPP-ACP is achieved by stablizing high concentration of calcium ions. The relatively high calcium concentration in plaque inhibits the cariogenic bacteria adhesion to the acquired films and their mutual adhesion, as well as influenced the bacteria metabolism by invaliding the calcium pump in bacteria membrane. In order to verify the remineralization effect of CPP-ACP, we compared the prepared 5% CPP-ACP and the traditional 2% NaCl solution in experiment 2, as well as the quantity and depth of demineralization by microphotography technology. The results showed that 5% CPP-ACP had significant anticariogenic effect compared with the deionized water, which is equivalent to 2% NaF. CPP-ACP is safe, effective, non-toxic or side effects, and can avoid the side effect by local high concentration of fluoride. CPP-ACP has a more wide range of applicable crowd and age than fluorine and it’s the ideal succedaneum of the latter.
Researches had shown that the calcium and phosphorus ions of CPP-ACP diffused into the subsurface enamel relatively easier by transforming into the neutral ions and ion-pairs. In the early demineralized enamel lesions and lesion surface, the balance between the charged ions and neutral ions maintained the activity gradient of neutral-ion diffused into the lesion. Therefore, CPP-ACP had the obvious advantage in the remineralization of subsurface enamel lesions, but there were few reports about what level of demineralization could be remineralized or the effect after the fluorine was added. Aiming at this question, we used a series CPP-ACP solution of three concentrations in experiment 3 and the fluoride was added into the solution to form CPP-ACFP. By microradiation quantitative study, the results showed that the remineralization throughout the body of subsurface enamel demineralizated lesions was dose-dependent with the CPP-ACP; the added F significantly improved the remineralization ability of CPP-ACP which acts as the vector of the F, which solved the problem of poor permeability of fluorde.
In traditional studies, the mineralization solution containing of calcium phosphate and fluoride formed a layer of mineral deposition in the enamel surface, covering the original enamel surface, reaching a so-called "remineralization effect" rather than the recovery of the original enamel structure. In order to verify whether there was any advantage of stablized calcium, phosphate and fluoride ions of CPP in this aspect, we used scanning electron microscopy to observe the surface morphology changes of the enamel (experiment4), the results showed that the newly formed minerals by remineralization of CPP-ACP had a dense structure, forming an organic combination with the surviving structure of the enamel; With the CPP-ACP concentration increased, the surface of the enamel gradually became smoother, the damage of the surface structure caused by acid was gradually restored, the overall smooth appearance was recovered, which was similar to the normal enamel. While NaF and saturated solution of calcium phosphate depositing in the enamel surface in the form of a large number of non-uniform and loose granular, which had loose structure and rough surface. Because of uneven deposition, the pits contour of corrosion was still faintly visible. The morphology observation of the current experiment confirmed the obvious advantages of CPP-ACP in remineralization once again.
In recent years, with the increasing consumption of acidic beverages such as fruit juice, great concerns have been taken on the demineralization of the enamel caused by the acid in food. This kind of acid is relatively low in pH (2.0~4.0), and differs in the mode of enamel corrosion and subsurface demineralizion; a 1~4μm softened layer formed in the surface of enamel. This softened enamel layer is easily removed by outside force such as teeth brushing. So it is important to protect the destroyed surface by remineralization on the softened surface of enamel. In order to further detect the nano-structure of the softened enamel surface, we used citric acid of pH 3.25 to etch the enamel surface for a short time (120s) in experiment 5. After the softened surface formed, we used CPP-ACP for remineralization, observed the role of remineralization using Atomic Force Microscope (AFM) for the first time and measured the roughness of the enamel surface. The results show that, by remineralization of CPP-ACP, extensing the original crystal structure on the etched enamel surface orderly arranged a lot of micro-crystal fiber (enamel structure of the third level) which was in same size and directions.These micro-fiber lens were parallel to each other and linked closely to further reunion assembled into crystal fiber bundle (the fourth-class structure of enamel), which repaired the destruction part of the enamel surface and reduced the surface roughness caused by etching. This experiment provided the theoretical evidences to verify that the CPP-ACP could restore the nano-structure of the destroyed enamel.
The incidence of white spot during the application of fixed appliance is significantly higher than others, and the white spot remains to be chalk colored many years postorthodontic treatment, which affects the patients’appearance. Clinically there is no effective way to cure the already suffered white spot and the traditional fluoride curing the white spot has effect only in a small range, there is no report about completely curing the white spot. In experiment 6, we first used commercialized CPP-ACP (Tooth Mousse) to treat the long existent enamel white spot and quantitative the decrease of white spot. By intraoral colored photos, clinical observation and Image-Pro Plus image analyze software; the white spot and the ratio of the white spot were measured. The paired t test was used to compare among pretreatment, 1 month and 2 months posttreatment. The results showed the surface ot the white spots decreased by 5.456±2.358% 1 month posttreatment and 8.359±3.472 2 months later compared with that of pr-treatment, which was significantly decreased (P<0.01). Part of the patients received treatment as long as 10 months, and the white spots was fully remineralized. The appearance and color returned to normal, which increased the aesthetic effect of the teeth. The quantitative study of the white spots surface by the intraoral photos which was also called the computer assistant image analyzing is reliability and repeatability.
By the vitro and vivo study, we proposed that CPP-ACP could stablize the high concentration of calcium and phosphate in aqueous solution without deposition. With the concentration of CPP increased the level of agglomeration of CPP-ACP nano-structure decreases and uniform in distribution. When appropriate concentration of fluorine was added, the diameter of nano-structure was further decreased; 5%CPP-ACP could inhibit the enamel demineralization in vitro, the effect of which was equal to 2% NaCl; CPP-ACP in vitro could make the already demineralized subsurface enamel remineralized, which was dependent on the concentration, and at the same time restored the destroyed enamel surface to normal and had synergy effect with fluoride; The further study by AFM confirmed that the remineralization effect of CPP-ACP was not simply the deposition of minerals, but by the remineralization of enamel crystal and gradually assembling, restored the nano-structure of enamel; The CPP-ACP used to treat white spot after the orthodontic treatment had satisfactory clinical effect, which further confirmed the remineralization effect of CPP-ACP.
The innovation of the experiment compared with the relative study of domestic and alien are as follows:①Laser particle size analyzer was used to study the particle diameter and the distribution of different concentration of CPP-ACP and the results supplied the new evidences to the remineralization effect of CPP-ACP and its concentration relevance;②For the first time , Atomic Force Microscope was used to study the remineralization effect of CPP-ACP and observe the enamel remineralizaiton in the nano level; which showed that CPP-ACP could restore the enamel nano-structure by remineralization, providing the evidences for the CPP-ACP clinical using for remineralization treatment.③Commercialized CPP-ACP was used to treat the orthodontic white spots, which broke the situation of having nothing to do with orthodontic white spots and made a new way for treatment. In addition, in alien studies, CPP-ACP was usually used at a low concentrate (0.1%～1%), while this current experiment we increased the concentration to 5% and added 500mg·L~(-1) NaCl into the CPP-ACP aqueous solution, which formed stable CPP-ACP and CPP-ACFP solution characteristic of good anti-acid and remineralization effect; But in domestic researches of the feature of anti-acid and remineralization of CPP-ACP were limited and mainly focused on the surface of the enamel; this current experiment detected the changes of all layers of the demineralized legion caused by CPP-AC(F)P by Radiographic microscopy techniques, and the demineralization depth and quantity were quantitatively analyzed as well, which was the first time in our country and also one of our research features
已有研究证实CPP-ACP以纳米级晶簇的形式存在,而纳米级的粒子在溶液中极易发生团聚,但关于溶液中CPP-ACP微粒的群体存在状态却没有相关的研究报道,以往研究中使用的溶液浓度均为较低的浓度(0.1%~1%),本研究将CPP浓度提高到了5%,并相应增加钙磷浓度,得到了稳定的胶体状悬浮溶液。本研究通过对CPP-ACP溶液的粒度及粒径分布的检测,发现了在水溶液中CPP-ACP纳米晶簇以团聚的状态保持平衡,并且随CPP浓度的增加,团聚的程度越小,适当的氟离子在一定程度上可以减小这种团聚。通过显微放射照相技术量化研究了CPP-ACP在体外的抗酸和再矿化效果,结果表明,5%CPP-ACP具有显著的提高釉质抗酸性的作用,与20g/L氟化钠相似;而不同浓度的CPP-ACP在体外均可引起釉质表层下脱矿病损全层的均质性再矿化,再矿化程度呈浓度依赖性;同时对表层的釉质的SEM观察,发现CPP-ACP对表层下修复的同时还将表层釉质的破坏复原了,并且新形成的矿物质与残余釉质结构有机的结合,使修复后的釉质表面结构紧密、光滑平整,类似于正常釉质;在软化釉质模型中应用AFM观察了CPP-ACP的再矿化作用,结果显示在CPP-ACP的作用下,受损釉质的纳米结构得到了修复;将CPP-ACP应用于临床正畸后白斑的治疗,图像分析结果证实CPP-ACP可以显著减小白斑的面积,经过较长时间的使用,可以完全消除白斑,提高正畸的美学效果。
Under normal circumstances, the enamel demineralization and remineralization maintain a dynamic equilibrium. The use of fixed appliance leads this equilibrium to the enamel demineralization. So the patients received the orthodontic treatment are often of high risk to enamel demineralization. Early demineralization of the enamel mostly confined to the surface layer. If the appliance was removed in time, it would impossible to be restored by saliva remineralization. However, at most of the time, when the appliance couldn’t be removed immediately, the demineralization of surface layer gradually develops to the subsurface layer, forming the visible white spots. At this time, the lesion is only the loss of the minerals of local enamel, which hasn’t caused the destructure and has the possibility of restoring the demineralized enamel. However, the remineralization capacity of saliva is very limited at this time, and needs other measures to remineralize. The key of remineralization is the supplement of losted calcium phosphate ions, but because of the low dissolubility of the calcium phosphate, it is difficult to maintain high concentration bioavailable ions at the surface of the enamel to diffuse into the subsurface enamel. So in traditional methods, calcium and phosphate could only be use at very low concentration. Methods of remineralization are also limited to the enamel surface laeyer by minerals depositing. But there’s little effect of restoration in the enamel inner lesion. Therefore, seeking the ways of restoration of the demineralization subsurface layer rather than masked by modification is always the ideal goal of the scholars.
The finding of casein phospho-peptides has brought a new breakthrough in this research field. Casein phosphopeptides (CPPs), a bioactive peptide, which is rich of phosphoserine, is obtained from milk casein by hydrolization, segregation and purification. It has the significant capacity of stablizing the calcium and phosphate ions in solution, and can combine with a big amount of calcium, phosphate and OH- ions before spontaneous precipitation under neutral or alkalinity circumstances. So in CPPs aqueous solution, the stablized calcium and phosphate is 100 times more than usual, and forming the nanocomplex, casein phosphopeptide-amorphous calcium phosphate (CPP-ACP). CPP-ACP, acts as the good vector for calcium and phosphate ions, not only supply ions, but also prevent them from spontaneous pricipitation at the enamel surface. Often it promotes the ions diffuse into the subsurface enamel in an appropriate molar ratio. The unique nature of CPP-ACP makes it have a broad application prospects in remineralization of demineralized enamel.
Some Scholars had proposed that the CPP combinated with the forming ACP, forming a metastable solution. The researches of CPP-ACP nano micro particle have also confirmed its status of non-crystal. But as the micro-particles of the nano level easily tend to gather together in the solution, there’s no relative report of domestic or alien about whether the CPP-ACP particles form together in the solution and the state of existing, as well as the effect of concentration or fluoride. In order to investigate the feature of the particle size distribution and the existant form of nanoparticles groups, we used the laser particle size analysis in experiment 1. By measuring the size and distribution of the CPP-ACP, its aggregation and stability is reflected. By increasing the concentration of 0.1%~1% in usual experiment to 5%, as the result, we got stable 5% CPP-ACP colloidal solution, and with the concentration of CPP increases, the particle size of CPP-ACP gradually decreased and the distribution became narrow. When appropriate concentration of fluoride ions added, the particel size was further reduced, showing that in solution of high concentration, the nanoclusters dispersed better, gather together less, and the size and distribution was relatively uniform. The remeasurement carried out one month later showed that the size and distribution of particle had no significantly change, which confirmed that the CPP-ACP solution in the current experiment was relatively stable which laid the foundation for the followed-up experiments.
The remineralization effect of CPP-ACP is achieved by stablizing high concentration of calcium ions. The relatively high calcium concentration in plaque inhibits the cariogenic bacteria adhesion to the acquired films and their mutual adhesion, as well as influenced the bacteria metabolism by invaliding the calcium pump in bacteria membrane. In order to verify the remineralization effect of CPP-ACP, we compared the prepared 5% CPP-ACP and the traditional 2% NaCl solution in experiment 2, as well as the quantity and depth of demineralization by microphotography technology. The results showed that 5% CPP-ACP had significant anticariogenic effect compared with the deionized water, which is equivalent to 2% NaF. CPP-ACP is safe, effective, non-toxic or side effects, and can avoid the side effect by local high concentration of fluoride. CPP-ACP has a more wide range of applicable crowd and age than fluorine and it’s the ideal succedaneum of the latter.
Researches had shown that the calcium and phosphorus ions of CPP-ACP diffused into the subsurface enamel relatively easier by transforming into the neutral ions and ion-pairs. In the early demineralized enamel lesions and lesion surface, the balance between the charged ions and neutral ions maintained the activity gradient of neutral-ion diffused into the lesion. Therefore, CPP-ACP had the obvious advantage in the remineralization of subsurface enamel lesions, but there were few reports about what level of demineralization could be remineralized or the effect after the fluorine was added. Aiming at this question, we used a series CPP-ACP solution of three concentrations in experiment 3 and the fluoride was added into the solution to form CPP-ACFP. By microradiation quantitative study, the results showed that the remineralization throughout the body of subsurface enamel demineralizated lesions was dose-dependent with the CPP-ACP; the added F significantly improved the remineralization ability of CPP-ACP which acts as the vector of the F, which solved the problem of poor permeability of fluorde.
In traditional studies, the mineralization solution containing of calcium phosphate and fluoride formed a layer of mineral deposition in the enamel surface, covering the original enamel surface, reaching a so-called "remineralization effect" rather than the recovery of the original enamel structure. In order to verify whether there was any advantage of stablized calcium, phosphate and fluoride ions of CPP in this aspect, we used scanning electron microscopy to observe the surface morphology changes of the enamel (experiment4), the results showed that the newly formed minerals by remineralization of CPP-ACP had a dense structure, forming an organic combination with the surviving structure of the enamel; With the CPP-ACP concentration increased, the surface of the enamel gradually became smoother, the damage of the surface structure caused by acid was gradually restored, the overall smooth appearance was recovered, which was similar to the normal enamel. While NaF and saturated solution of calcium phosphate depositing in the enamel surface in the form of a large number of non-uniform and loose granular, which had loose structure and rough surface. Because of uneven deposition, the pits contour of corrosion was still faintly visible. The morphology observation of the current experiment confirmed the obvious advantages of CPP-ACP in remineralization once again.
In recent years, with the increasing consumption of acidic beverages such as fruit juice, great concerns have been taken on the demineralization of the enamel caused by the acid in food. This kind of acid is relatively low in pH (2.0~4.0), and differs in the mode of enamel corrosion and subsurface demineralizion; a 1~4μm softened layer formed in the surface of enamel. This softened enamel layer is easily removed by outside force such as teeth brushing. So it is important to protect the destroyed surface by remineralization on the softened surface of enamel. In order to further detect the nano-structure of the softened enamel surface, we used citric acid of pH 3.25 to etch the enamel surface for a short time (120s) in experiment 5. After the softened surface formed, we used CPP-ACP for remineralization, observed the role of remineralization using Atomic Force Microscope (AFM) for the first time and measured the roughness of the enamel surface. The results show that, by remineralization of CPP-ACP, extensing the original crystal structure on the etched enamel surface orderly arranged a lot of micro-crystal fiber (enamel structure of the third level) which was in same size and directions.These micro-fiber lens were parallel to each other and linked closely to further reunion assembled into crystal fiber bundle (the fourth-class structure of enamel), which repaired the destruction part of the enamel surface and reduced the surface roughness caused by etching. This experiment provided the theoretical evidences to verify that the CPP-ACP could restore the nano-structure of the destroyed enamel.
The incidence of white spot during the application of fixed appliance is significantly higher than others, and the white spot remains to be chalk colored many years postorthodontic treatment, which affects the patients’appearance. Clinically there is no effective way to cure the already suffered white spot and the traditional fluoride curing the white spot has effect only in a small range, there is no report about completely curing the white spot. In experiment 6, we first used commercialized CPP-ACP (Tooth Mousse) to treat the long existent enamel white spot and quantitative the decrease of white spot. By intraoral colored photos, clinical observation and Image-Pro Plus image analyze software; the white spot and the ratio of the white spot were measured. The paired t test was used to compare among pretreatment, 1 month and 2 months posttreatment. The results showed the surface ot the white spots decreased by 5.456±2.358% 1 month posttreatment and 8.359±3.472 2 months later compared with that of pr-treatment, which was significantly decreased (P<0.01). Part of the patients received treatment as long as 10 months, and the white spots was fully remineralized. The appearance and color returned to normal, which increased the aesthetic effect of the teeth. The quantitative study of the white spots surface by the intraoral photos which was also called the computer assistant image analyzing is reliability and repeatability.
By the vitro and vivo study, we proposed that CPP-ACP could stablize the high concentration of calcium and phosphate in aqueous solution without deposition. With the concentration of CPP increased the level of agglomeration of CPP-ACP nano-structure decreases and uniform in distribution. When appropriate concentration of fluorine was added, the diameter of nano-structure was further decreased; 5%CPP-ACP could inhibit the enamel demineralization in vitro, the effect of which was equal to 2% NaCl; CPP-ACP in vitro could make the already demineralized subsurface enamel remineralized, which was dependent on the concentration, and at the same time restored the destroyed enamel surface to normal and had synergy effect with fluoride; The further study by AFM confirmed that the remineralization effect of CPP-ACP was not simply the deposition of minerals, but by the remineralization of enamel crystal and gradually assembling, restored the nano-structure of enamel; The CPP-ACP used to treat white spot after the orthodontic treatment had satisfactory clinical effect, which further confirmed the remineralization effect of CPP-ACP.
The innovation of the experiment compared with the relative study of domestic and alien are as follows:①Laser particle size analyzer was used to study the particle diameter and the distribution of different concentration of CPP-ACP and the results supplied the new evidences to the remineralization effect of CPP-ACP and its concentration relevance;②For the first time , Atomic Force Microscope was used to study the remineralization effect of CPP-ACP and observe the enamel remineralizaiton in the nano level; which showed that CPP-ACP could restore the enamel nano-structure by remineralization, providing the evidences for the CPP-ACP clinical using for remineralization treatment.③Commercialized CPP-ACP was used to treat the orthodontic white spots, which broke the situation of having nothing to do with orthodontic white spots and made a new way for treatment. In addition, in alien studies, CPP-ACP was usually used at a low concentrate (0.1%～1%), while this current experiment we increased the concentration to 5% and added 500mg·L~(-1) NaCl into the CPP-ACP aqueous solution, which formed stable CPP-ACP and CPP-ACFP solution characteristic of good anti-acid and remineralization effect; But in domestic researches of the feature of anti-acid and remineralization of CPP-ACP were limited and mainly focused on the surface of the enamel; this current experiment detected the changes of all layers of the demineralized legion caused by CPP-AC(F)P by Radiographic microscopy techniques, and the demineralization depth and quantity were quantitatively analyzed as well, which was the first time in our country and also one of our research features
引文
[1]葛俊,崔福斋,吉宁.人牙釉质分级结构的观察[J].牙体牙髓牙周病学杂志, 2006,16(2):61-66.
[2] Yanagisawa T. Crystalline structure of enamel in carious lesion.[J]. JJpnDentAss, 1997,46:1167-1176.
[3] Thylstrup A. [Diagnosis and therapy of early caries. 1][J]. Phillip J Restaur Zahnmed, 1987,4(4):228-235.
[4] Melrose CA, Appleton J, Lovius BB. A scanning electron microscopic study of early enamel caries formed in vivo beneath orthodontic bands[J]. Br J Orthod, 1996,23(1):43-47.
[5] Al-Khateeb S, Forsberg CM, de Josselin de Jong E, et al. A longitudinal laser fluorescence study of white spot lesions in orthodontic patients[J]. Am J Orthod Dentofacial Orthop, 1998,113(6):595-602.
[6] Nunn JH. Prevalence of dental erosion and the implications for oral health[J]. Eur J Oral Sci, 1996,104(2 ( Pt 2)):156-161.
[7] Eisenburger M, Shellis RP, Addy M. Scanning electron microscopy of softened enamel[J]. Caries Res, 2004,38(1):67-74.
[8]葛俊.牙釉质和骨的分级结构和纳米力学性能研究[D].北京:清华大学材料科学与工程系, 2005.
[9] Lippert F, Parker DM, Jandt KD. Toothbrush abrasion of surface softened enamel studied with tapping mode AFM and AFM nanoindentation[J]. Caries Res, 2004,38(5):464-472.
[10] Cross KJ, Huq NL, Palamara JE, et al. Physicochemical characterization of casein phosphopeptide-amorphous calcium phosphate nanocomplexes[J]. J Biol Chem, 2005,280(15):15362-15369.
[11] Larsen MJ, Fejerskov O. Chemical and structural challenges in remineralization of dental enamel lesions[J]. Scand J Dent Res, 1989,97(4):285-296.
[12] Rose RK. Binding characteristics of Streptococcus mutans for calcium andcasein phosphopeptide[J]. Caries Res, 2000,34(5):427-431.
[13] Reynolds EC, Cain CJ, Webber FL, et al. Anticariogenicity of calcium phosphate complexes of tryptic casein phosphopeptides in the rat[J]. J Dent Res, 1995,74(6):1272-1279.
[14] Black J, Hastings G. Handbook of biomeaterial properties.[M]. London: Chapman & Hall. UK., 1998.
[15] Byode A, John W. Dental Enamel. Proceedings of a Symposium. London, 23-25 April 1996[J]. Ciba Found Symp, 1997,205:1-284.
[16] Mann S. The biomimetics of enamel: a paradigm for organized biomaterials synthesis[J]. Ciba Found Symp, 1997,205:261-274.
[17] Spears IR. A three-dimensional finite element model of prismatic enamel: a re-appraisal of the data on the Young's modulus of enamel[J]. J Dent Res, 1997,76(10):1690-1697.
[18] Farina M, Schemmel A, Weissmuller G, et al. Atomic force microscopy study of tooth surfaces[J]. J Struct Biol, 1999,125(1):39-49.
[19]赵玮,汪说之,洪汉烈等.人牙釉质、牙本质矿化成分的晶体特性[J].中华口腔医学杂志, 2002,37(3):219-221.
[20] Marshall AF, Lawless KR. TEM study of the central dark line in enamel crystallites[J]. J Dent Res, 1981,60(10):1773-1782.
[21] Shibahara H, Tohda H, Yanagizawa T. High resolution electron microscopic observation of hydroxyapatite in tooth crystals[J]. J Electron Microsc (Tokyo), 1994,43(2):89-94.
[22] LeGeros RZ. Calcium phosphates in oral biology and medicine[J]. Monogr Oral Sci, 1991,15:1-201.
[23] LeGeros RZ, Sakae T, Bautista C, et al. Magnesium and carbonate in enamel and synthetic apatites[J]. Adv Dent Res, 1996,10(2):225-231.
[24] Boyde A. Carbonate concentration, crystal centers, core dissolution, caries, cross striations, circadian rhythms, and compositional contrast in the SEM[J]. J Dent Res, 1979,58(Spec Issue B):981-983.
[25] Robinson C, Kirkham J, Brookes SJ, et al. The chemistry of enameldevelopment[J]. Int J Dev Biol, 1995,39(1):145-152.
[26] Risnes S. Growth tracks in dental enamel[J]. J Hum Evol, 1998,35(4-5):331-350.
[27] Lees S, Bonar LC, Mook HA. a study of dense mineralized tissue by neutron diffracion[J]. IntJBiol Macromol, 1984,22:181-187.
[28] Popowics TE, Rensberger JM, Herring SW. Enamel microstructure and microstrain in the fracture of human and pig molar cusps[J]. Arch Oral Biol, 2004,49(8):595-605.
[29] Nishikawa S. Correlation of the arrangement pattern of enamel rods and secretory ameloblasts in pig and monkey teeth: a possible role of the terminal webs in ameloblast movement during secretion[J]. Anat Rec, 1992,232(4):466-478.
[30] Kawai N. Comparative anatomy of the bands of Schreger[J]. Okajimas Folia Anat Jpn, 1955,27(2-3):115-131.
[31] Boyde A. Electron microscopic observations relating to the nature and development of prism decussation in mammalian dental enamel[J]. Bull Group Int Rech Sci Stomatol, 1969,12(2):151-207.
[32] Cui FZ, Ge J. New observations of the hierarchical structure of human enamel, from nanoscale to microscale[J]. J Tissue Eng Regen Med, 2007,1(3):185-191.
[33] Fincham AG, Moradian-Oldak J, Simmer JP. The structural biology of the developing dental enamel matrix[J]. J Struct Biol, 1999,126(3):270-299.
[34] Oliver W, Pharr G. An improved techniuqe fordetemrining hardness and elastic modulus using load and displacement sensing indentation experiments.[J]. J Mater Res, 1992,7:1546-1578.
[35] Pharr G, Oliver W. Measurement of thin film mechanical properties using nanindentation.[J]. MRS Bull, 1992,17:29-33.
[36] Habelitz S, Marshall SJ, Marshall GW, Jr., et al. Mechanical properties of human dental enamel on the nanometre scale[J]. Arch Oral Biol, 2001,46(2):173-183.
[37] Ge J, Cui FZ, Wang XM, et al. Property variations in the prism and the organic sheath within enamel by nanoindentation[J]. Biomaterials, 2005,26(16):3333-3339.
[38] Cuy JL, Mann AB, Livi KJ, et al. Nanoindentation mapping of the mechanical properties of human molar tooth enamel[J]. Arch Oral Biol, 2002,47(4):281-291.
[39] Remizov SM, Prujansky LY, Matveevsky RM. Wear resistance and microhardness of human teeth[J]. Proc Inst Mech Eng [H], 1991,205(3):201-202.
[40] Willems G, Celis JP, Lambrechts P, et al. Hardness and Young's modulus determined by nanoindentation technique of filler particles of dental restorative materials compared with human enamel[J]. J Biomed Mater Res, 1993,27(6):747-755.
[41] Frank RM. Structural events in the caries process in enamel, cementum, and dentin[J]. J Dent Res, 1990,69:559-566.
[42] Featherstone JD, Goodman P, McLean JD. Electron miscroscope study of defect zones in dental enamel[J]. J Ultrastruct Res, 1979,67(2):117-123.
[43] Featherstone JD, Nelson DG, McLean JD. An electron microscope study of modifications to defect regions in dental enamel and synthetic apatites[J]. Caries Res, 1981,15(4):278-288.
[44] Yanagisawa T, Miake Y. High-resolution electron microscopy of enamel-crystal demineralization and remineralization in carious lesions[J]. J Electron Microsc (Tokyo), 2003,52(6):605-613.
[45] Jongebloed WL, Molenaar I, Arends J. Morphology and size-distribution of sound and acid-treated enamel crystallites[J]. Calcif Tissue Res, 1975,19(2):109-123.
[46] Yanagisawa T, Takuma S, Tohda H, et al. High resolution electron microscopy of enamel crystals in cases of human dental fluorosis[J]. J Electron Microsc (Tokyo), 1989,38(6):441-448.
[47] Tohda H, Yanagisawa T, Tanaka N, et al. Growth and fusion of apatitecrystals in the remineralized enamel.[J]. J Electron Microsc, 1990,39(4):238-244.
[48] Tanaka N. [High resolution electron microscopy of the crystalline structure in remineralized enamel][J]. Shikwa Gakuho, 1989,89(9):1441-1478.
[49] Tohda H. [Forming mechanism of subsurface carious lesions in enamel ][J]. Shikwa Gakuho, 1981,81(3):421-444.
[50] Silverstone LM. The significance of remineralization in caries prevention[J]. J Can Dent Assoc, 1984,50(2):157-167.
[51] Silverstone LM, Wefel JS, Zimmerman BF, et al. Remineralization of natural and artificial lesions in human dental enamel in vitro. Effect of calcium concentration of the calcifying fluid[J]. Caries Res, 1981,15(2):138-157.
[52] Kawasaki K, Tanaka Y, Takagi O. Crystallographic analysis of demineralized human enamel treated by laser-irradiation or remineralization[J]. Arch Oral Biol, 2000,45(9):797-804.
[53] Lustman J, Sela M, Sela J, et al. White enamel patches. A scanning electron microscope study of approximal surfaces following removal of plaque[J]. J Oral Pathol, 1974,3(3):123-126.
[54] Fejerskov O, Josephsen K, Nyvad B. Surface ultrastructure of unerupted mature human enamel[J]. Caries Res, 1984,18(4):302-314.
[55] Nelson DG, Featherstone JD, Duncan JF, et al. Effect of carbonate and fluoride on the dissolution behaviour of synthetic apatites[J]. Caries Res, 1983,17(3):200-211.
[56] Carlstroem D. Polarization Microscopy of Dental Enamel with Reference to Incipient Carious Lesions[J]. Adv Oral Biol, 1964,1:255-296.
[57] Orams HJ, Phakey PP, Rachinger WA, et al. Ultrastructural changes in the translucent and dark zones of early enamel caries[J]. J Oral Pathol, 1980,9(1):54-61.
[58] Carstensen W. The effects of different phosphoric acid concentrations on surface enamel[J]. Angle Orthod, 1992,62(1):51-58.
[59] Diedrich P. Enamel alterations from bracket bonding and debonding: a study with the scanning electron microscope[J]. Am J Orthod, 1981,79(5):500-522.
[60] Darling AI. Some observations on amelogenesis imperfecta and calcification of the dental enamel[J]. Proc R Soc Med, 1956,49(10):759-765.
[61] Bergman G, Lind PO. A quantitative microradiographic study of incipient enamel caries[J]. J Dent Res, 1966,45(5):1477-1484.
[62] Besic FC, Knowles CR, Keller O, et al. Detailed electron probe microanalysis of three teeth sections with early enamel caries[J]. J Dent Res, 1970,49(1):111-118.
[63] Hallsworth AS, Robinson C, Weatherbell JA. Mineral and magnesium distribution within the approximal carious lesion of dental enamel[J]. Caries Res, 1972,6(2):156-168.
[64] Arends J, Christoffersen J. The nature of early caries lesions in enamel[J]. J Dent Res, 1986,65(1):2-11.
[65] Borsboom PC, vd Mei HC, Arends J. Enamel lesion formation with and without 0.12 ppm F in solution[J]. Caries Res, 1985,19(5):396-402.
[66] White GE. Dental caries: a bacterial energy crisis[J]. N Y State Dent J, 1974,40(7):420-421.
[67] Kidd EA, Thylstrup A, Fejerskov O, et al. Histopathology of caries-like lesions created in vitro in fluorosed and sound enamel[J]. Caries Res, 1978,12(5):268-274.
[68] Lagerweij MD, ten Cate JM. Remineralisation of enamel lesions with daily applications of a high-concentration fluoride gel and a fluoridated toothpaste: an in situ study[J]. Caries Res, 2002,36(4):270-274.
[69] Anderson P, Elliott JC. Coupled diffusion as basis for subsurface demineralization in dental caries[J]. Caries Res, 1987,21(6):522-525.
[70] Anderson P, Bollet-Quivogne FR, Dowker SE, et al. Demineralization in enamel and hydroxyapatite aggregates at increasing ionic strengths[J].Arch Oral Biol, 2004,49(3):199-207.
[71] Anderson P, Elliott JC. Subsurface demineralization in dental enamel and other permeable solids during acid dissolution[J]. J Dent Res, 1992,71(8):1473-1481.
[72] Ando M, Hall AF, Eckert GJ, et al. Relative ability of laser fluorescence techniques to quantitate early mineral loss in vitro[J]. Caries Res, 1997,31(2):125-131.
[73] Ten Bosch JJ, Angmar-Mansson B. A review of quantitative methods for studies of mineral content of intra-oral caries lesions[J]. J Dent Res, 1991,70(1):2-14.
[74] Kawasaki K, Ruben J, Stokroos I, et al. The remineralization of EDTA-treated human dentine[J]. Caries Res, 1999,33(4):275-280.
[75] Kashani H, Birkhed D, Arends J, et al. Effect of toothpicks with and without fluoride on De- and remineralization of enamel and dentine in situ[J]. Caries Res, 1998,32(6):422-427.
[76] Skrtic D, Hailer AW, Takagi S, et al. Quantitative assessment of the efficacy of amorphous calcium phosphate/methacrylate composites in remineralizing caries-like lesions artificially produced in bovine enamel[J]. J Dent Res, 1996,75(9):1679-1686.
[77] Paddock SW. Principles and practices of laser scanning confocal microscopy[J]. Mol Biotechnol, 2000,16(2):127-149.
[78] Gonzalez-Cabezas C, Fontana M, Dunipace AJ, et al. Measurement of enamel remineralization using microradiography and confocal microscopy. A correlational study[J]. Caries Res, 1998,32(5):385-392.
[79] Tranaeus S, Shi XQ, Lindgren LE, et al. In vivo repeatability and reproducibility of the quantitative light-induced fluorescence method[J]. Caries Res, 2002,36(1):3-9.
[80] Shi XQ, Tranaeus S, Angmar-Mansson B. Comparison of QLF and DIAGNOdent for quantification of smooth surface caries[J]. Caries Res, 2001,35(1):21-26.
[81] Ferreira Zandona AG, Analoui M, Beiswanger BB, et al. An in vitro comparison between laser fluorescence and visual examination for detection of demineralization in occlusal pits and fissures[J]. Caries Res, 1998,32(3):210-218.
[82] Lussi A, Jaeggi T, Jaeggi-Scharer S. Prediction of the erosive potential of some beverages[J]. Caries Res, 1995,29(5):349-354.
[83] Featherstone JD, ten Cate JM, Shariati M, et al. Comparison of artificial caries-like lesions by quantitative microradiography and microhardness profiles[J]. Caries Res, 1983,17(5):385-391.
[84] Lussi A, Jaggi T, Scharer S. The influence of different factors on in vitro enamel erosion[J]. Caries Res, 1993,27(5):387-393.
[85] Lippert F, Parker DM, Jandt KD. In vitro demineralization/remineralization cycles at human tooth enamel surfaces investigated by AFM and nanoindentation[J]. J Colloid Interface Sci, 2004,280(2):442-448.
[86] Schaad P, Paris E, Cuisinier FJ, et al. Atomic force microscopy study of human tooth enamel surfaces[J]. Scanning Microsc, 1993,7(4):1149-1152.
[87]马淑媛,蔡继业,吴扬哲.原子力显微镜对牙体硬组织的纳米结构分析[J].中国组织工程研究与临床康复, 2007,11(18):3536-3539.
[88] Frank L, David MP, Klaus DJ. In situ remineralization of surface softened human enamel studied with AFM nanoindentation[J]. Surface Science, 2004,553(3):105-114.
[89] Nytun RB, Raadal M, Espelid I. Diagnosis of dentin involvement in occlusal caries based on visual and radiographic examination of the teeth[J]. Scand J Dent Res, 1992,100(3):144-148.
[90] Creanor SL, Russell JI, Strang DM, et al. The prevalence of clinically undetected occlusal dentine caries in Scottish adolescents[J]. Br Dent J, 1990,169(5):126-129.
[91] Wenzel A, Fejerskov O. Validity of diagnosis of questionable caries lesions in occlusal surfaces of extracted third molars[J]. Caries Res, 1992,26(3):188-194.
[92] Schneiderman A, Elbaum M, Shultz T, et al. Assessment of dental caries with Digital Imaging Fiber-Optic TransIllumination (DIFOTI): in vitro study[J]. Caries Res, 1997,31(2):103-110.
[93] Lussi A, Firestone A, Schoenberg V, et al. In vivo diagnosis of fissure caries using a new electrical resistance monitor[J]. Caries Res, 1995,29(2):81-87.
[94] Thylstrup A, Bille J, Qvist V. Radiographic and observed tissue changes in approximal carious lesions at the time of operative treatment[J]. Caries Res, 1986,20(1):75-84.
[95] Reynolds EC, Johnson IH. Effect of milk on caries incidence and bacterial composition of dental plaque in the rat[J]. Arch Oral Biol, 1981,26(5):445-451.
[96] Rosen S, Min DB, Harper DS, et al. Effect of cheese, with and without sucrose, on dental caries and recovery of Streptococcus mutans in rats[J]. J Dent Res, 1984,63(6):894-896.
[97] Harper DS, Osborn JC, Hefferren JJ, et al. Cariostatic evaluation of cheeses with diverse physical and compositional characteristics[J]. Caries Res, 1986,20(2):123-130.
[98] Harper DS, Osborn JC, Clayton R, et al. Modification of food cariogenicity in rats by mineral-rich concentrates from milk[J]. J Dent Res, 1987,66(1):42-45.
[99] Krobicka A, Bowen WH, Pearson S, et al. The effects of cheese snacks on caries in desalivated rats[J]. J Dent Res, 1987,66(6):1116-1119.
[100] Silva MF, Burgess RC, Sandham HJ, et al. Effects of water-soluble components of cheese on experimental caries in humans[J]. J Dent Res, 1987,66(1):38-41.
[101] Reynolds EC, del Rio A. Effect of casein and whey-protein solutions on caries experience and feeding patterns of the rat[J]. Arch Oral Biol, 1984,29(11):927-933.
[102] Reynolds EC, Black CL. Confectionery composition and rat caries[J].Caries Res, 1987,21(6):538-545.
[103] Reynolds EC, Black CL. Reduction of cholate's cariogenicity by supplementation with sodium caseinate[J]. Caries Res, 1987,21(5):445-451.
[104] Reynolds EC. The prevention of sub-surface demineralization of bovine enamel and change in plaque composition by casein in an intra-oral model[J]. J Dent Res, 1987,66(6):1120-1127.
[105] Reynolds EC, Black CL. Cariogenicity of a confection supplemented with sodium caseinate at a palatable level[J]. Caries Res, 1989,23(5):368-370.
[106] Shaw JH. Effects of dietary composition on tooth decay in the albino rat[J]. J Nutr, 1950,41(1):13-24.
[107] Bavetta LA, Mc CF. Protein factors and experimental rat caries[J]. J Nutr, 1957,63(1):107-117.
[108] Murray MJ, Snowden MJ. The preparation, characterization and applications of colloidal microgels.[J]. Advance in Colloid and Interface Science, 1995,54:73-91.
[109] Boggs LJ, M. R, Bike SG. Characterization and rheological investigation of polymer microgels used in automotive coatings[J]. Journal of Coating Technology, 1996,68(855):63-74.
[110] Sasa N, Yamaoka T. Surface-activated photopolymer microgels.[J]. Advanced Materials, 1994,6(5):417-421.
[111] Bergbreiter DE, Case BL, Liu YS, et al. Poly(N-isopeopylacrylamide) soluble polymer supports in catalysis and synthsis.[J]. macromolecules, 1998,31(18):6053-6062.
[112] Arndt KF, Kucklin D, Richter A. Application of sensitive hydrogels in flow control.[J]. Polymers for Advanced Technologies, 2000,11(12):496-505.
[113] Bawendi MG, Carroll PJ, Wilson WL, et al. Luminescence properties of CdSe quantum crystallites-resonance between interior and surface localized states.[J]. Journal of Chemical Physics, 1992,96(2):946-954.
[114] Antonietti M, Grohn F, Hartmann J, et al. Nonclassical shapes ofnoble-metal colloids by synthesis in microgel nanoreactors.[J]. Augewandte Chemie-Inernational Edition In English, 1997,36(19):2080-2083.
[115] Blanco A, Lopez C, Mayoral R, et al. CdS photoluminescence inhibition by a photonic structure.[J]. Applied Physics Letters, 1998,73(13):1781-1783.
[116]汪玉松,邹思湘.乳生物化学[M].吉林:吉林大学出版社, 1995.
[117]张和平,张列兵.现代乳品手册[M].北京:中国轻工业出版社, 2005.
[118] Xu SQ, Zhang JG, Paquet G, et al. From hybrid microgels to photonic crystals.[J]. Advance Functional Materials, 2003,13(6):468-472.
[119] Elaissari A, Ganachaud F, Pichot C. Biorelevant latexes and microgels for the interaction with nucleic acids.[J]. Colloid Chemistry, 2003,227:169-193.
[120] Chen LY, Pemondetto GE, Subirade M. Food protein-based materials as nurraceutical delivery systems.[J]. Trends in Food Science & Technology, 2006,17(5):272-283.
[121] McGann TC, Kearney RD, Buchheim W, et al. Amorphous calcium phosphate in casein micelles of bovine milk[J]. Calcif Tissue Int, 1983,35(6):821-823.
[122] McGann TC, Buchheim W, Kearney RD, et al. Composition and ultrastructure of calcium phosphate-citrate complexes in bovine milk systems[J]. Biochim Biophys Acta, 1983,760(3):415-420.
[123] Holt C, Wahlgren NM, Drakenberg T. Ability of a beta-casein phosphopeptide to modulate the precipitation of calcium phosphate by forming amorphous dicalcium phosphate nanoclusters[J]. Biochem J, 1996,314 ( Pt 3):1035-1039.
[124] Reynolds EC, Black CL, Cai F, et al. Advances in enamel remineralization: casein phosphopeptide-amorphous calcium phosphate. [J]. J Clin Dent, 1999(10):86-88.
[125] Ametani A, Kaminogawa S, Shimizu M, et al. Rapid screening of antigenically reactive fragments of alpha s1-casein using HPLC andELISA[J]. J Biochem, 1987,102(2):421-425.
[126] Meisel H, Frister H. Chemical characterization of a caseinophosphopeptide isolated from in vivo digests of a casein diet[J]. Biol Chem Hoppe Seyler, 1988,369(12):1275-1279.
[127] Reynolds EC, Riley PF, Adamson NJ. A selective precipitation purification procedure for multiple phosphoseryl-containing peptides and methods for their identification[J]. Anal Biochem, 1994,217(2):277-284.
[128] Cross KJ, Huq NL, Bicknell W, et al. Cation-dependent structural features of beta-casein-(1-25)[J]. Biochem J, 2001,356(1):277-286.
[129] Huq NL, Cross KJ, Reynolds EC. A 1H NMR study of the casein phosphopeptideαS2-casein(1-21)[J]. J Dent Res, 1995,74:579.
[130] Huq NL, Cross KJ, Reynolds EC. A 1H-NMR study of the casein phosphopeptide alpha s1-casein(59-79)[J]. Biochim Biophys Acta, 1995,1247(2):201-208.
[131] Ferraretto A, Gravaghi C, Fiorilli A, et al. Casein-derived bioactive phosphopeptides: role of phosphorylation and primary structure in promoting calcium uptake by HT-29 tumor cells[J]. FEBS Lett, 2003,551(1-3):92-98.
[132] Cross KJ, Huq NL, Reynolds EC. Casein phosphopeptides in oral health--chemistry and clinical applications[J]. Curr Pharm Des, 2007,13(8):793-800.
[133] Marsh ME. Binding of calcium and phosphate ions to dentin phosphophoryn[J]. Biochemistry, 1989,28(1):346-352.
[134] Green MR, Pastewka JV, Peacock AC. Differential staining of phosphoproteins on polyacrylamide gels with a cationic carbocyanine dye[J]. Anal Biochem, 1973,56(1):43-51.
[135] Cross KJ, Huq NL, Reynolds E, C,. Anticariogenic peptides. In: Nutraceutical proteins and peptides in health and disease[J]. CRC Press, 2005:335-351.
[136] Naito H. CPP: intro-intestenal formation and significance in thebioavailability of calcium.[J]. Japan Sci Press, 1990:207-220.
[137] Reeves RE, Latour NG. Calcium phosphate sequestering phosphopeptide from casein[J]. Science, 1958,128(3322):472.
[138] Reynolds EC. Remineralization of enamel subsurface lesions by casein phosphopeptide-stabilized calcium phosphate solutions[J]. J Dent Res, 1997,76(9):1587-1595.
[139] van Dijk JW, Borggreven JM, Driessens FC. Diffusion in mammalian tooth enamel in relation to the caries process[J]. Arch Oral Biol, 1983,28(7):591-597.
[140] Borggreven JM, van Dijk JW, Driessens FC. Effect of mono- and divalent ions on diffusion and binding in bovine tooth enamel[J]. Arch Oral Biol, 1981,26(8):663-669.
[141] Cross KJ, Huq NL, Stanton DP, et al. NMR studies of a novel calcium, phosphate and fluoride delivery vehicle-alpha(S1)-casein(59-79) by stabilized amorphous calcium fluoride phosphate nanocomplexes[J]. Biomaterials, 2004,25(20):5061-5069.
[142] ten Cate JM, van Loveren C. Fluoride mechanisms[J]. Dent Clin North Am, 1999,43(4):713-742, vii.
[143] Cochrane NJ, Saranathan S, Cai F, et al. Enamel subsurface lesion remineralisation with casein phosphopeptide stabilised solutions of calcium, phosphate and fluoride[J]. Caries Res, 2008,42(2):88-97.
[144] Gray JA. Kinetics of enamel dissolution during formation of incipient caries-like lesions[J]. Arch Oral Biol, 1966,11(4):397-422.
[145] Holly FJ, Gray JA. Mechanism for incipient carious lesion growth utilizing a physical model based on diffusion concepts[J]. Arch Oral Biol, 1968,13(3):319-334.
[146] Featherstone JD, Rodgers BE, Smith MW. Physicochemical requirements for rapid remineralization of early carious lesions[J]. Caries Res, 1981,15(3):221-235.
[147] Reynolds EC, Cai F, Shen P, et al. Retention in plaque andremineralization of enamel lesions by various forms of calcium in a mouthrinse or sugar-free chewing gum[J]. J Dent Res, 2003,82(3):206-211.
[148] Buchalla W, Attin T, Schulte-Monting J, et al. Fluoride uptake, retention, and remineralization efficacy of a highly concentrated fluoride solution on enamel lesions in situ[J]. J Dent Res, 2002,81(5):329-333.
[149] O'Reilly MM, Featherstone JD. Demineralization and remineralization around orthodontic appliances: an in vivo study[J]. Am J Orthod Dentofacial Orthop, 1987,92(1):33-40.
[150] Ogaard B, Rolla G, Arends J. Orthodontic appliances and enamel demineralization. Part 1. Lesion development[J]. Am J Orthod Dentofacial Orthop, 1988,94(1):68-73.
[151] Glatz EGM, Featherstone JDB. Demineralization related to orthodontic bands and brackets-clinical study[J]. Am J Orthod Dentofacial Orthop, 1985,1985:87.
[152]王翰章.中华口腔科学(上卷)[M].北京:人民卫生出版社, 2001.
[153] Mattingly JA, Sauer GJ, Yancey JM, et al. Enhancement of Streptococcus mutans colonization by direct bonded orthodontic appliances[J]. J Dent Res, 1983,62(12):1209-1211.
[154]陈梅红,李璞,尚斌.正畸与正常儿童龋活性比较[J].口腔医学纵横, 2002,18(1):28-29.
[155] Jordan C, LeBlanc DJ. Influences of orthodontic appliances on oral populations of mutans streptococci[J]. Oral Microbiol Immunol, 2002,17(2):65-71.
[156] Chang HS, Walsh LJ, Freer TJ. The effect of orthodontic treatment on salivary flow, pH, buffer capacity, and levels of mutans streptococci and lactobacilli[J]. Aust Orthod J, 1999,15(4):229-234.
[157]潘一春,张丁,傅民魁.口腔正畸学[M].北京:人民卫生出版社, 2003.
[158]王翰章.中华口腔科学(中卷)[M].北京:人民卫生出版社, 2001.
[159] Geiger AM, Gorelick L, Gwinnett AJ, et al. The effect of a fluorideprogram on white spot formation during orthodontic treatment[J]. Am J Orthod Dentofacial Orthop, 1988,93(1):29-37.
[160] Zimmer BW, Rottwinkel Y. Assessing patient-specific decalcification risk in fixed orthodontic treatment and its impact on prophylactic procedures[J]. Am J Orthod Dentofacial Orthop, 2004,126(3):318-324.
[161] Sonis AL, Snell W. An evaluation of a fluoride-releasing, visible light-activated bonding system for orthodontic bracket placement[J]. Am J Orthod Dentofacial Orthop, 1989,95(4):306-311.
[162] Hobson RS, Clark JD. How UK orthodontists advise patients on oral hygiene[J]. Br J Orthod, 1998,25(1):64-66.
[163] Lees A, Rock WP. A comparison between written, verbal, and videotape oral hygiene instruction for patients with fixed appliances[J]. J Orthod, 2000,27(4):323-328.
[164] Zimmer B. Systematic decalcification prophylaxis during treatment with fixed appliances[J]. J Orofac Orthop, 1999,60(3):205-214.
[165] Sengun A, Sari Z, Ramoglu SI, et al. Evaluation of the dental plaque pH recovery effect of a xylitol lozenge on patients with fixed orthodontic appliances[J]. Angle Orthod, 2004,74(2):240-244.
[166] Mattick CR, Mitchell L, Chadwick SM, et al. Fluoride-releasing elastomeric modules reduce decalcification: a randomized controlled trial[J]. J Orthod, 2001,28(3):217-219.
[167] Doherty UB, Benson PE, Higham SM. Fluoride-releasing elastomeric ligatures assessed with the in situ caries model[J]. Eur J Orthod, 2002,24(4):371-378.
[168] Trent JM, Bittner M, Zhang J, et al. Use of microgenomic technology for analysis of alterations in DNA copy number and gene expression in malignant melanoma[J]. Clin Exp Immunol, 1997,107 Suppl 1:33-40.
[169] Rawls HR, Zimmerman BF. Fluoride-exchanging resins for caries protection[J]. Caries Res, 1983,17(1):32-43.
[170] Chung CK, Millett DT, Creanor SL, et al. Fluoride release and cariostaticability of a compomer and a resin-modified glass ionomer cement used for orthodontic bonding[J]. J Dent, 1998,26(5-6):533-538.
[171] Capilouto ML, DePaola PF, Gron P. In vivo study of slow-release fluoride resin and enamel uptake[J]. Caries Res, 1990,24(6):441-445.
[172] Gorton J, Featherstone JD. In vivo inhibition of demineralization around orthodontic brackets[J]. Am J Orthod Dentofacial Orthop, 2003,123(1):10-14.
[173] Glasspoole EA, Erickson RL, Davidson CL. Demineralization of enamel in relation to the fluoride release of materials[J]. Am J Dent, 2001,14(1):8-12.
[174] Corry A, Millett DT, Creanor SL, et al. Effect of fluoride exposure on cariostatic potential of orthodontic bonding agents: an in vitro evaluation[J]. J Orthod, 2003,30(4):323-329.
[175]胡炜,王勤,傅民魁.口腔正畸固定矫治器应用中釉质脱矿的临床调查[J].口腔正畸学, 2001,8(2):51-54.
[176] Attin T, Knofel S, Buchalla W, et al. In situ evaluation of different remineralization periods to decrease brushing abrasion of demineralized enamel[J]. Caries Res, 2001,35(3):216-222.
[177] Marinelli CB, Donly KJ, Wefel JS, et al. An in vitro comparison of three fluoride regimens on enamel remineralization[J]. Caries Res, 1997,31(6):418-422.
[178] Eakle WS, Featherstone JD, Weintraub JA, et al. Salivary fluoride levels following application of fluoride varnish or fluoride rinse[J]. Community Dent Oral Epidemiol, 2004,32(6):462-469.
[179] Yamamoto H, Ooya K. Potential of yttrium-aluminum-garnet laser in caries prevention[J]. J Oral Pathol, 1974,3(1):7-15.
[180] Bahar A, Tagomori S. The effect of normal pulsed Nd-YAG laser irradiation on pits and fissures in human teeth[J]. Caries Res, 1994,28(6):460-467.
[181] Forsberg CM, Oliveby A, Lagerlof F. Salivary clearance of sugar beforeand after insertion of fixed orthodontic appliances[J]. Am J Orthod Dentofacial Orthop, 1992,102(6):527-530.
[182] Gorelick L, Geiger AM, Gwinnett AJ. Incidence of white spot formation after bonding and banding[J]. Am J Orthod, 1982,81(2):93-98.
[183] Mitchell L. Decalcification during orthodontic treatment with fixed appliances--an overview[J]. Br J Orthod, 1992,19(3):199-205.
[184] Yoshihara A, Sakuma S, Kobayashi S, et al. Antimicrobial effect of fluoride mouthrinse on mutans streptococci and lactobacilli in saliva[J]. Pediatr Dent, 2001,23(2):113-117.
[185] Willmot DR. White lesions after orthodontic treatment: does low fluoride make a difference?[J]. J Orthod, 2004,31(3):235-242.
[186] Linton JL. Quantitative measurements of remineralization of incipient caries[J]. Am J Orthod Dentofacial Orthop, 1996,110(6):590-597.
[187] Chang HS, Walsh LJ, Freer TJ. Enamel demineralization during orthodontic treatment. Aetiology and prevention[J]. Aust Dent J, 1997,42(5):322-327.
[188] Lagerweij MD, Damen JJM, Stookey GK. Remineralization of small lesions by ?uoride. [J]. J Dent Res 1997,76(Spec Issue):16.
[189] Brostek AM, Bochenek AJ, Walsh LJ. Minimally invasive dentistry: a review and update[J]. Shanghai Kou Qiang Yi Xue, 2006,15(3):225-249.
[190] Rose RK. Effects of an anticariogenic casein phosphopeptide on calcium diffusion in streptococcal model dental plaques[J]. Arch Oral Biol, 2000,45(7):569-575.
[191] Iijima Y, Cai F, Shen P, et al. Acid resistance of enamel subsurface lesions remineralized by a sugar-free chewing gum containing casein phosphopeptide-amorphous calcium phosphate[J]. Caries Res, 2004,38(6):551-556.
[192] Pourghadiri M, Longhurst P, Watson TF. A new technique for the controlled removal of mottled enamel: measurement of enamel loss[J]. Br Dent J, 1998,184(5):239-241.
[193] Welbury RR, Carter NE. The hydrochloric acid-pumice microabrasion technique in the treatment of post-orthodontic decalcification[J]. Br J Orthod, 1993,20(3):181-185.
[194] Croll TP, Cavanaugh RR. Enamel color modification by controlled hydrochloric acid-pumice abrasion. I. technique and examples[J]. Quintessence Int, 1986,17(2):81-87.
[195] Murphy TC, Willmot DR, Rodd HD. Management of postorthodontic demineralized white lesions with microabrasion: a quantitative assessment[J]. Am J Orthod Dentofacial Orthop, 2007,131(1):27-33.
[196] Kendall RL. Hydrochloric acid removal of brown fluorosis stains: clinical and scanning electron micrographic observations.[J]. Quintessence Int, 1990,20(837-839)
[197] Jacobsson-Hunt U, M.R. H. The effect of hydrochloric acid pumice abrasion on fluorosed teeth.[J]. J Dent Res, 1989,86:982.
[198] Peariasamy K, Anderson P, Brook AH. A quantitative study of the effect of pumicing and etching on the remineralization of enamel opacities.[J]. Int J Pediatr Dent, 2001,11:193-200.
[199] Ardu S, Castioni NV, Benbachir N, et al. Minimally invasive treatment of white spot enamel lesions[J]. Quintessence Int, 2007,38(8):633-636.
[200] Reddy MB, Love M. The impact of food processing on the nutritional quality of vitamins and minerals[J]. Adv Exp Med Biol, 1999,459:99-106.
[201]王芸,唐荣银.五倍子水提取物处理早期人工釉质龋后表面结构的扫描电镜观察[J].牙体牙髓牙周病学杂志, 2005,15(3):133-135.
[202]戴啊师,蔡春木,海蓝.天然药物隔山消促进釉质再矿化的研究[J].牙体牙髓牙周病学杂志, 2007,17(11):635-637.
[203]吕奎龙,滕立群.纳米羟基磷灰石牙膏对人工龋再矿化研究[J].黑龙江医药科学, 2002,25:26-27.
[204] Sculean A, Windisch P, Keglevich T, et al. Clinical and histologic evaluation of human intrabony defects treated with an enamel matrix protein derivative combined with a bovine-derived xenograft[J]. Int JPeriodontics Restorative Dent, 2003,23(1):47-55.
[205]王志伟,赵月萍,周长忍, et al.牙釉基质蛋白诱导牙釉质的再矿化研究[J].四川大学学报(医学版), 2008,39(4):579-582.
[206]刘洪涛,葛世荣,朱华.微粒团聚的尺度效应及其分形表征[J].润滑与密封, 2006,184:1-3.
[207]陈洪云,金斌,董英杰等.测定钛白粉平均粒径的研究[J].涂料工业, 2002(6):40-41.
[208]王振新,钱彦虎.激光粒度仪测定PTA粒径分布[J].聚酯工业, 2004(3):50-53.
[209] Holt C, Timmins PA, Errington N, et al. A core-shell model of calcium phosphate nanoclusters stabilized by beta-casein phosphopeptides, derived from sedimentation equilibrium and small-angle X-ray and neutron-scattering measurements[J]. Eur J Biochem, 1998,252(1):73-78.
[210] Hung CH, Hsung MN. Dispersion and stabilization of aqueous TiO 2 suspension.[J]. Ceramics International, 1995(21):65-68.
[211] Brown LR, White JO, Horton IM, et al. Effect of continuous fluoride gel use on plaque fluoride retention and microbial activity[J]. J Dent Res, 1983,62(6):746-751.
[212]盛江筠,刘正.变形链球菌耐氟菌株的诱导及产酸性的实验研究[J].中华口腔医学杂志, 2000,35(2):95-98.
[213] Vacca-Smith AM, Bowen WH. The effect of milk and kappa casein on streptococcal glucosyltransferase[J]. Caries Res, 1995,29(6):498-506.
[214] Schupbach P, Neeser JR, Golliard M, et al. Incorporation of caseinoglycomacropeptide and caseinophosphopeptide into the salivary pellicle inhibits adherence of mutans streptococci[J]. J Dent Res, 1996,75(10):1779-1788.
[215]刘兴容,徐皑李,李艳萍等.酪蛋白磷酸肽钙磷复合体对变形链球菌产酸影响的实验研究[J].现代口腔医学杂志, 2007,21(1):63-65.
[216] Nishioka Y, Nakagaki H, Kato S, et al. Fluoride profiles in different sites of approximal surfaces of second primary molars after topical application ofacidulated phosphate fluoride gel in vivo[J]. Arch Oral Biol, 1995,40(12):1157-1161.
[217] Riordan PJ. Dental fluorosis, dental caries and fluoride exposure among 7-year-olds[J]. Caries Res, 1993,27(1):71-77.
[218] Koch G, Bergmann-Arnadottir I, Bjarnason S, et al. Caries-preventive effect of fluoride dentifrices with and without anticalculus agents: a 3-year controlled clinical trial[J]. Caries Res, 1990,24(1):72-79.
[219] Pine CM, ten Bosch JJ. Dynamics of and diagnostic methods for detecting small carious lesions[J]. Caries Res, 1996,30(6):381-388.
[220] Mallon DE, Mellberg JR. Analysis of dental hard tissue by computerized microdensitometry[J]. J Dent Res, 1985,64(2):112-116.
[221]刘艳辉.显微放射摄影术在口腔科的应用[J].上海医学, 2001,24(增刊):66-68.
[222] Fontana M, Li Y, Dunipace AJ, et al. Measurement of enamel demineralization using microradiography and confocal microscopy. A correlation study[J]. Caries Res, 1996,30(5):317-325.
[223] Iijima Y, Takagi O, Duschner H, et al. Influence of nail varnish on the remineralization of enamel single sections assessed by microradiography and confocal laser scanning microscopy[J]. Caries Res, 1998,32(5):393-400.
[224]叶应妩,王毓三.全国临床检验操作规程[M].江苏:东南大学出版社, 1997.
[225]马美范,王介峰.用硫酸亚铁铵作还原剂测定食品中磷含量[J].广西轻工业, 1998(1):43-45.
[226] Reynolds EC. Anticariogenic complexes of amorphous calcium phosphate stabilized by casein phosphopeptides: a review[J]. Spec Care Dentist, 1998,18(1):8-16.
[227] Mazzaoui SA, Burrow MF, Tyas MJ, et al. Incorporation of casein phosphopeptide-amorphous calcium phosphate into a glass-ionomer cement[J]. J Dent Res, 2003,82(11):914-918.
[228]赵琼芝,蔡璠.酪蛋白磷酸多肽钙氟磷复合体对牙釉质缺损再矿化的实验研究[J].中华口腔医学杂志, 2001,36 (6):421-423.
[229] Arends J, Christoffersen J. Nature and role of loosely bound fluoride in dental caries[J]. J Dent Res, 1990,69 Spec No:601-605; discussion 634-606.
[230] Reynolds EC, Cai F, Cochrane NJ, et al. Fluoride and casein phosphopeptide-amorphous calcium phosphate[J]. J Dent Res, 2008,87(4):344-348.
[231] Tucker K, Adams M, Shaw L, et al. Human enamel as a substrate for in vitro acid dissolution studies: influence of tooth surface and morphology[J]. Caries Res, 1998,32(2):135-140.
[232] Kirkham J, Robinson C, Strong M, et al. Effects of frequency and duration of acid exposure on demineralization/remineralization behaviour of human enamel in vitro[J]. Caries Res, 1994,28(1):9-13.
[233] Meurman JH, Frank RM. Progression and surface ultrastructure of in vitro caused erosive lesions in human and bovine enamel[J]. Caries Res, 1991,25(2):81-87.
[234] Shellis RP. A scanning electron-microscopic study of solubility variations in human enamel and dentine[J]. Arch Oral Biol, 1996,41(5):473-484.
[235] Larsen MJ, Richards A. The influence of saliva on the formation of calcium fluoride-like material on human dental enamel[J]. Caries Res, 2001,35(1):57-60.
[236]吴红崑,周学东,谭红.微量元素矿化液促进釉质龋再矿化层形成机理的研究[J].华西口腔医学杂志, 2000,18(4):219-221.
[237] Damato FA, Strang R, Stephen KW. Effect of fluoride concentration on remineralization of carious enamel: an in vitro pH-cycling study[J]. Caries Res, 1990,24(3):174-180.
[238]陈向群,王勤.氟化钠和再矿化液对早期釉质龋作用的电子探针和显微硬度研究[J].北京医科大学学报, 1995,27(4):287.
[239] Craig GG. The use of a calcium sucrose phosphates-calciumorthophosphate complex as a cariostatic agent[J]. Br Dent J, 1975,138(1):25-28.
[240] Prescott B, Renugopalakrishnan V, Glimcher MJ, et al. A Raman spectroscopic study of hen egg yolk phosvitin: structures in solution and in the solid state[J]. Biochemistry, 1986,25(10):2792-2798.
[241] Crossley M, Huq NL, Kirszbaum L, et al. Purification and N-terminal sequence analysis of bovine dentine phosphophoryn[J]. J Dent Res, 1996,75(spec Iss):154.
[242] Sorensen ES, Petersen TE. Identification of two phosphorylation motifs in bovine osteopontin[J]. Biochem Biophys Res Commun, 1994,198(1):200-205.
[243] Binning G, Quate CF, Gerber C. Atomic force microscope[J]. J Phys Rev Lett, 1986,56(9):930-933.
[244]宗仁鹤,白春礼,李振刚, et al.原子力显微镜及其在激光作用DNA的结构研究中的作用[J].量子电子学, 1996,13(2):107-112.
[245]陈宝安,杜鹃.原子力显微镜在临床医学中的应用[J].现代医学仪器与应用, 2003,15(4):7-10.
[246]张英鸽,饶曼人.原子力显微技术的基本原理及药理学应用[J].药学学报, 2000,35(6):476-480.
[247] Chang L, Kious T, Yorgancioglu M, et al. Cytoskeleton of living, unstained cells imaged by scanning force microscopy[J]. Biophys J, 1993,64(4):1282-1286.
[248] Kasas S, Gotzos V, Celio MR. Observation of living cells using the atomic force microscope[J]. Biophys J, 1993,64(2):539-544.
[249] Radmacher M, Tillamnn RW, Fritz M, et al. From molecules to cells: imaging soft samples with the atomic force microscope[J]. Science, 1992,257(5078):1900-1905.
[250] Hoh JH, Lal R, John SA, et al. Atomic force microscopy and dissection of gap junctions[J]. Science, 1991,253(5026):1405-1408.
[251] Butt HJ, Downing KH, Hansma PK. Imaging the membrane proteinbacteriorhodopsin with the atomic force microscope[J]. Biophys J, 1990,58(6):1473-1480.
[252] Soon LL, Bottema C, Breed WG. Atomic force microscopy and cytochemistry of chromatin from marsupial spermatozoa with special reference to Sminthopsis crassicaudata[J]. Mol Reprod Dev, 1997,48(3):367-374.
[253] Lal R. Imaging molecular structure of channels and receptors with an atomic force microscope[J]. Scanning Microsc Suppl, 1996,10:81-95; discussion 95-86.
[254] Arakawa H, Umemura K, Ikai A. Protein images obtained by STM, AFM and TEM[J]. Nature, 1992,358(6382):171-173.
[255] Yip CM, Yip CC, Ward MD. Direct force measurements of insulin monomer-monomer interactions[J]. Biochemistry, 1998,37(16):5439-5449.
[256] Merkel R, Nassoy P, Leung A, et al. Energy landscapes of receptor-ligand bonds explored with dynamic force spectroscopy[J]. Nature, 1999,397(6714):50-53.
[257] Hoyt PR, Doktycz MJ, Warmack RJ, et al. Spin-column isolation of DNA-protein interactions from complex protein mixtures for AFM imaging[J]. Ultramicroscopy, 2001,86(1-2):139-143.
[258] Jiao Y, Cherny DI, Heim G, et al. Dynamic interactions of p53 with DNA in solution by time-lapse atomic force microscopy[J]. J Mol Biol, 2001,314(2):233-243.
[259] Dammer U, Hegner M, Anselmetti D, et al. Specific antigen/antibody interactions measured by force microscopy[J]. Biophys J, 1996,70(5):2437-2441.
[260] Willemsen OH, Snel MM, Cambi A, et al. Biomolecular interactions measured by atomic force microscopy[J]. Biophys J, 2000,79(6):3267-3281.
[261] Hinterdorfer P, Baumgartner W, Gruber HJ, et al. Detection and localization of individual antibody-antigen recognition events by atomicforce microscopy[J]. Proc Natl Acad Sci U S A, 1996,93(8):3477-3481.
[262] Willemsen OH, Snel MM, van der Werf KO, et al. Simultaneous height and adhesion imaging of antibody-antigen interactions by atomic force microscopy[J]. Biophys J, 1998,75(5):2220-2228.
[263] Schwesinger F, Ros R, Strunz T, et al. Unbinding forces of single antibody-antigen complexes correlate with their thermal dissociation rates[J]. Proc Natl Acad Sci U S A, 2000,97(18):9972-9977.
[264] Smulson ME, Pang D, Jung M, et al. Irreversible binding of poly(ADP)ribose polymerase cleavage product to DNA ends revealed by atomic force microscopy: possible role in apoptosis[J]. Cancer Res, 1998,58(16):3495-3498.
[265] Ellis DJ, Dryden DT, Berge T, et al. Direct observation of DNA translocation and cleavage by the EcoKI endonuclease using atomic force microscopy[J]. Nat Struct Biol, 1999,6(1):15-17.
[266] Valle M, Valpuesta JM, Carrascosa JL, et al. The interaction of DNA with bacteriophage phi 29 connector: a study by AFM and TEM[J]. J Struct Biol, 1996,116(3):390-398.
[267] Habelitz S, Balooch M, Marshall SJ, et al. In situ atomic force microscopy of partially demineralized human dentin collagen fibrils[J]. J Struct Biol, 2002,138(3):227-236.
[268] Habelitz S, Marshall SJ, Marshall GW, Jr., et al. The functional width of the dentino-enamel junction determined by AFM-based nanoscratching[J]. J Struct Biol, 2001,135(3):294-301.
[269] Hegedus C, Bistey T, Flora-Nagy E, et al. An atomic force microscopy study on the effect of bleaching agents on enamel surface[J]. J Dent, 1999,27(7):509-515.
[270] ten Cate JM, Imfeld T. Dental erosion, summary[J]. Eur J Oral Sci, 1996,104(2):241-244.
[271] Zachrisson BJ. A posttreatment evaluation of direct bonding in orthodontics[J]. Am J Orthod, 1977,71(2):173-189.
[272] Sudjalim TR, Woods MG, Manton DJ, et al. Prevention of demineralization around orthodontic brackets in vitro[J]. Am J Orthod Dentofacial Orthop, 2007,131(6):701-709.
[273] Mizrahi E. Enamel demineralization following orthodontic treatment[J]. Am J Orthod, 1982,82(1):62-67.
[274] von der FF. The effect of fluorides on the caries resistance of enamel[J]. Acta Odontol Scand, 1961,19:431-442.
[275] Downer MC. Caries prevalence in the United Kingdom[J]. Int Dent J, 1994,44(4 Suppl 1):365-370.
[276] Marcusson A, Norevall LI, Persson M. White spot reduction when using glass ionomer cement for bonding in orthodontics: a longitudinal and comparative study[J]. Eur J Orthod, 1997,19(3):233-242.
[277] Derrick W. White Spot Lesions After Orthodontic Treatment[J]. Seminars in Orthodontics, 2008,14(3):209-219.
[278] Banks PA, Richmond S. Enamel sealants: a clinical evaluation of their value during fixed appliance therapy[J]. Eur J Orthod, 1994,16(1):19-25.
[279] Mizrahi E. Surface distribution of enamel opacities following orthodontic treatment[J]. Am J Orthod, 1983,84(4):323-331.
[280] Benson PE, Pender N, Higham SM, et al. Morphometric assessment of enamel demineralisation from photographs[J]. J Dent, 1998,26(8):669-677.
[281] Benson PE, Pender N, Higham SM. Enamel demineralisation assessed by computerised image analysis of clinical photographs[J]. J Dent, 2000,28(5):319-326.
[282] Willmot DR, Benson PE, Pender N, et al. Reproducibility of quantitative measurement of white enamel demineralisation by image analysis[J]. Caries Res, 2000,34(2):175-181.
[283] Christoffersen J, christoffersen MR, Johansen T. Kinetics of growth and dissolution of fluorapatite[J]. J Cryst Growth, 1996,163:295-303.
[2] Yanagisawa T. Crystalline structure of enamel in carious lesion.[J]. JJpnDentAss, 1997,46:1167-1176.
[3] Thylstrup A. [Diagnosis and therapy of early caries. 1][J]. Phillip J Restaur Zahnmed, 1987,4(4):228-235.
[4] Melrose CA, Appleton J, Lovius BB. A scanning electron microscopic study of early enamel caries formed in vivo beneath orthodontic bands[J]. Br J Orthod, 1996,23(1):43-47.
[5] Al-Khateeb S, Forsberg CM, de Josselin de Jong E, et al. A longitudinal laser fluorescence study of white spot lesions in orthodontic patients[J]. Am J Orthod Dentofacial Orthop, 1998,113(6):595-602.
[6] Nunn JH. Prevalence of dental erosion and the implications for oral health[J]. Eur J Oral Sci, 1996,104(2 ( Pt 2)):156-161.
[7] Eisenburger M, Shellis RP, Addy M. Scanning electron microscopy of softened enamel[J]. Caries Res, 2004,38(1):67-74.
[8]葛俊.牙釉质和骨的分级结构和纳米力学性能研究[D].北京:清华大学材料科学与工程系, 2005.
[9] Lippert F, Parker DM, Jandt KD. Toothbrush abrasion of surface softened enamel studied with tapping mode AFM and AFM nanoindentation[J]. Caries Res, 2004,38(5):464-472.
[10] Cross KJ, Huq NL, Palamara JE, et al. Physicochemical characterization of casein phosphopeptide-amorphous calcium phosphate nanocomplexes[J]. J Biol Chem, 2005,280(15):15362-15369.
[11] Larsen MJ, Fejerskov O. Chemical and structural challenges in remineralization of dental enamel lesions[J]. Scand J Dent Res, 1989,97(4):285-296.
[12] Rose RK. Binding characteristics of Streptococcus mutans for calcium andcasein phosphopeptide[J]. Caries Res, 2000,34(5):427-431.
[13] Reynolds EC, Cain CJ, Webber FL, et al. Anticariogenicity of calcium phosphate complexes of tryptic casein phosphopeptides in the rat[J]. J Dent Res, 1995,74(6):1272-1279.
[14] Black J, Hastings G. Handbook of biomeaterial properties.[M]. London: Chapman & Hall. UK., 1998.
[15] Byode A, John W. Dental Enamel. Proceedings of a Symposium. London, 23-25 April 1996[J]. Ciba Found Symp, 1997,205:1-284.
[16] Mann S. The biomimetics of enamel: a paradigm for organized biomaterials synthesis[J]. Ciba Found Symp, 1997,205:261-274.
[17] Spears IR. A three-dimensional finite element model of prismatic enamel: a re-appraisal of the data on the Young's modulus of enamel[J]. J Dent Res, 1997,76(10):1690-1697.
[18] Farina M, Schemmel A, Weissmuller G, et al. Atomic force microscopy study of tooth surfaces[J]. J Struct Biol, 1999,125(1):39-49.
[19]赵玮,汪说之,洪汉烈等.人牙釉质、牙本质矿化成分的晶体特性[J].中华口腔医学杂志, 2002,37(3):219-221.
[20] Marshall AF, Lawless KR. TEM study of the central dark line in enamel crystallites[J]. J Dent Res, 1981,60(10):1773-1782.
[21] Shibahara H, Tohda H, Yanagizawa T. High resolution electron microscopic observation of hydroxyapatite in tooth crystals[J]. J Electron Microsc (Tokyo), 1994,43(2):89-94.
[22] LeGeros RZ. Calcium phosphates in oral biology and medicine[J]. Monogr Oral Sci, 1991,15:1-201.
[23] LeGeros RZ, Sakae T, Bautista C, et al. Magnesium and carbonate in enamel and synthetic apatites[J]. Adv Dent Res, 1996,10(2):225-231.
[24] Boyde A. Carbonate concentration, crystal centers, core dissolution, caries, cross striations, circadian rhythms, and compositional contrast in the SEM[J]. J Dent Res, 1979,58(Spec Issue B):981-983.
[25] Robinson C, Kirkham J, Brookes SJ, et al. The chemistry of enameldevelopment[J]. Int J Dev Biol, 1995,39(1):145-152.
[26] Risnes S. Growth tracks in dental enamel[J]. J Hum Evol, 1998,35(4-5):331-350.
[27] Lees S, Bonar LC, Mook HA. a study of dense mineralized tissue by neutron diffracion[J]. IntJBiol Macromol, 1984,22:181-187.
[28] Popowics TE, Rensberger JM, Herring SW. Enamel microstructure and microstrain in the fracture of human and pig molar cusps[J]. Arch Oral Biol, 2004,49(8):595-605.
[29] Nishikawa S. Correlation of the arrangement pattern of enamel rods and secretory ameloblasts in pig and monkey teeth: a possible role of the terminal webs in ameloblast movement during secretion[J]. Anat Rec, 1992,232(4):466-478.
[30] Kawai N. Comparative anatomy of the bands of Schreger[J]. Okajimas Folia Anat Jpn, 1955,27(2-3):115-131.
[31] Boyde A. Electron microscopic observations relating to the nature and development of prism decussation in mammalian dental enamel[J]. Bull Group Int Rech Sci Stomatol, 1969,12(2):151-207.
[32] Cui FZ, Ge J. New observations of the hierarchical structure of human enamel, from nanoscale to microscale[J]. J Tissue Eng Regen Med, 2007,1(3):185-191.
[33] Fincham AG, Moradian-Oldak J, Simmer JP. The structural biology of the developing dental enamel matrix[J]. J Struct Biol, 1999,126(3):270-299.
[34] Oliver W, Pharr G. An improved techniuqe fordetemrining hardness and elastic modulus using load and displacement sensing indentation experiments.[J]. J Mater Res, 1992,7:1546-1578.
[35] Pharr G, Oliver W. Measurement of thin film mechanical properties using nanindentation.[J]. MRS Bull, 1992,17:29-33.
[36] Habelitz S, Marshall SJ, Marshall GW, Jr., et al. Mechanical properties of human dental enamel on the nanometre scale[J]. Arch Oral Biol, 2001,46(2):173-183.
[37] Ge J, Cui FZ, Wang XM, et al. Property variations in the prism and the organic sheath within enamel by nanoindentation[J]. Biomaterials, 2005,26(16):3333-3339.
[38] Cuy JL, Mann AB, Livi KJ, et al. Nanoindentation mapping of the mechanical properties of human molar tooth enamel[J]. Arch Oral Biol, 2002,47(4):281-291.
[39] Remizov SM, Prujansky LY, Matveevsky RM. Wear resistance and microhardness of human teeth[J]. Proc Inst Mech Eng [H], 1991,205(3):201-202.
[40] Willems G, Celis JP, Lambrechts P, et al. Hardness and Young's modulus determined by nanoindentation technique of filler particles of dental restorative materials compared with human enamel[J]. J Biomed Mater Res, 1993,27(6):747-755.
[41] Frank RM. Structural events in the caries process in enamel, cementum, and dentin[J]. J Dent Res, 1990,69:559-566.
[42] Featherstone JD, Goodman P, McLean JD. Electron miscroscope study of defect zones in dental enamel[J]. J Ultrastruct Res, 1979,67(2):117-123.
[43] Featherstone JD, Nelson DG, McLean JD. An electron microscope study of modifications to defect regions in dental enamel and synthetic apatites[J]. Caries Res, 1981,15(4):278-288.
[44] Yanagisawa T, Miake Y. High-resolution electron microscopy of enamel-crystal demineralization and remineralization in carious lesions[J]. J Electron Microsc (Tokyo), 2003,52(6):605-613.
[45] Jongebloed WL, Molenaar I, Arends J. Morphology and size-distribution of sound and acid-treated enamel crystallites[J]. Calcif Tissue Res, 1975,19(2):109-123.
[46] Yanagisawa T, Takuma S, Tohda H, et al. High resolution electron microscopy of enamel crystals in cases of human dental fluorosis[J]. J Electron Microsc (Tokyo), 1989,38(6):441-448.
[47] Tohda H, Yanagisawa T, Tanaka N, et al. Growth and fusion of apatitecrystals in the remineralized enamel.[J]. J Electron Microsc, 1990,39(4):238-244.
[48] Tanaka N. [High resolution electron microscopy of the crystalline structure in remineralized enamel][J]. Shikwa Gakuho, 1989,89(9):1441-1478.
[49] Tohda H. [Forming mechanism of subsurface carious lesions in enamel ][J]. Shikwa Gakuho, 1981,81(3):421-444.
[50] Silverstone LM. The significance of remineralization in caries prevention[J]. J Can Dent Assoc, 1984,50(2):157-167.
[51] Silverstone LM, Wefel JS, Zimmerman BF, et al. Remineralization of natural and artificial lesions in human dental enamel in vitro. Effect of calcium concentration of the calcifying fluid[J]. Caries Res, 1981,15(2):138-157.
[52] Kawasaki K, Tanaka Y, Takagi O. Crystallographic analysis of demineralized human enamel treated by laser-irradiation or remineralization[J]. Arch Oral Biol, 2000,45(9):797-804.
[53] Lustman J, Sela M, Sela J, et al. White enamel patches. A scanning electron microscope study of approximal surfaces following removal of plaque[J]. J Oral Pathol, 1974,3(3):123-126.
[54] Fejerskov O, Josephsen K, Nyvad B. Surface ultrastructure of unerupted mature human enamel[J]. Caries Res, 1984,18(4):302-314.
[55] Nelson DG, Featherstone JD, Duncan JF, et al. Effect of carbonate and fluoride on the dissolution behaviour of synthetic apatites[J]. Caries Res, 1983,17(3):200-211.
[56] Carlstroem D. Polarization Microscopy of Dental Enamel with Reference to Incipient Carious Lesions[J]. Adv Oral Biol, 1964,1:255-296.
[57] Orams HJ, Phakey PP, Rachinger WA, et al. Ultrastructural changes in the translucent and dark zones of early enamel caries[J]. J Oral Pathol, 1980,9(1):54-61.
[58] Carstensen W. The effects of different phosphoric acid concentrations on surface enamel[J]. Angle Orthod, 1992,62(1):51-58.
[59] Diedrich P. Enamel alterations from bracket bonding and debonding: a study with the scanning electron microscope[J]. Am J Orthod, 1981,79(5):500-522.
[60] Darling AI. Some observations on amelogenesis imperfecta and calcification of the dental enamel[J]. Proc R Soc Med, 1956,49(10):759-765.
[61] Bergman G, Lind PO. A quantitative microradiographic study of incipient enamel caries[J]. J Dent Res, 1966,45(5):1477-1484.
[62] Besic FC, Knowles CR, Keller O, et al. Detailed electron probe microanalysis of three teeth sections with early enamel caries[J]. J Dent Res, 1970,49(1):111-118.
[63] Hallsworth AS, Robinson C, Weatherbell JA. Mineral and magnesium distribution within the approximal carious lesion of dental enamel[J]. Caries Res, 1972,6(2):156-168.
[64] Arends J, Christoffersen J. The nature of early caries lesions in enamel[J]. J Dent Res, 1986,65(1):2-11.
[65] Borsboom PC, vd Mei HC, Arends J. Enamel lesion formation with and without 0.12 ppm F in solution[J]. Caries Res, 1985,19(5):396-402.
[66] White GE. Dental caries: a bacterial energy crisis[J]. N Y State Dent J, 1974,40(7):420-421.
[67] Kidd EA, Thylstrup A, Fejerskov O, et al. Histopathology of caries-like lesions created in vitro in fluorosed and sound enamel[J]. Caries Res, 1978,12(5):268-274.
[68] Lagerweij MD, ten Cate JM. Remineralisation of enamel lesions with daily applications of a high-concentration fluoride gel and a fluoridated toothpaste: an in situ study[J]. Caries Res, 2002,36(4):270-274.
[69] Anderson P, Elliott JC. Coupled diffusion as basis for subsurface demineralization in dental caries[J]. Caries Res, 1987,21(6):522-525.
[70] Anderson P, Bollet-Quivogne FR, Dowker SE, et al. Demineralization in enamel and hydroxyapatite aggregates at increasing ionic strengths[J].Arch Oral Biol, 2004,49(3):199-207.
[71] Anderson P, Elliott JC. Subsurface demineralization in dental enamel and other permeable solids during acid dissolution[J]. J Dent Res, 1992,71(8):1473-1481.
[72] Ando M, Hall AF, Eckert GJ, et al. Relative ability of laser fluorescence techniques to quantitate early mineral loss in vitro[J]. Caries Res, 1997,31(2):125-131.
[73] Ten Bosch JJ, Angmar-Mansson B. A review of quantitative methods for studies of mineral content of intra-oral caries lesions[J]. J Dent Res, 1991,70(1):2-14.
[74] Kawasaki K, Ruben J, Stokroos I, et al. The remineralization of EDTA-treated human dentine[J]. Caries Res, 1999,33(4):275-280.
[75] Kashani H, Birkhed D, Arends J, et al. Effect of toothpicks with and without fluoride on De- and remineralization of enamel and dentine in situ[J]. Caries Res, 1998,32(6):422-427.
[76] Skrtic D, Hailer AW, Takagi S, et al. Quantitative assessment of the efficacy of amorphous calcium phosphate/methacrylate composites in remineralizing caries-like lesions artificially produced in bovine enamel[J]. J Dent Res, 1996,75(9):1679-1686.
[77] Paddock SW. Principles and practices of laser scanning confocal microscopy[J]. Mol Biotechnol, 2000,16(2):127-149.
[78] Gonzalez-Cabezas C, Fontana M, Dunipace AJ, et al. Measurement of enamel remineralization using microradiography and confocal microscopy. A correlational study[J]. Caries Res, 1998,32(5):385-392.
[79] Tranaeus S, Shi XQ, Lindgren LE, et al. In vivo repeatability and reproducibility of the quantitative light-induced fluorescence method[J]. Caries Res, 2002,36(1):3-9.
[80] Shi XQ, Tranaeus S, Angmar-Mansson B. Comparison of QLF and DIAGNOdent for quantification of smooth surface caries[J]. Caries Res, 2001,35(1):21-26.
[81] Ferreira Zandona AG, Analoui M, Beiswanger BB, et al. An in vitro comparison between laser fluorescence and visual examination for detection of demineralization in occlusal pits and fissures[J]. Caries Res, 1998,32(3):210-218.
[82] Lussi A, Jaeggi T, Jaeggi-Scharer S. Prediction of the erosive potential of some beverages[J]. Caries Res, 1995,29(5):349-354.
[83] Featherstone JD, ten Cate JM, Shariati M, et al. Comparison of artificial caries-like lesions by quantitative microradiography and microhardness profiles[J]. Caries Res, 1983,17(5):385-391.
[84] Lussi A, Jaggi T, Scharer S. The influence of different factors on in vitro enamel erosion[J]. Caries Res, 1993,27(5):387-393.
[85] Lippert F, Parker DM, Jandt KD. In vitro demineralization/remineralization cycles at human tooth enamel surfaces investigated by AFM and nanoindentation[J]. J Colloid Interface Sci, 2004,280(2):442-448.
[86] Schaad P, Paris E, Cuisinier FJ, et al. Atomic force microscopy study of human tooth enamel surfaces[J]. Scanning Microsc, 1993,7(4):1149-1152.
[87]马淑媛,蔡继业,吴扬哲.原子力显微镜对牙体硬组织的纳米结构分析[J].中国组织工程研究与临床康复, 2007,11(18):3536-3539.
[88] Frank L, David MP, Klaus DJ. In situ remineralization of surface softened human enamel studied with AFM nanoindentation[J]. Surface Science, 2004,553(3):105-114.
[89] Nytun RB, Raadal M, Espelid I. Diagnosis of dentin involvement in occlusal caries based on visual and radiographic examination of the teeth[J]. Scand J Dent Res, 1992,100(3):144-148.
[90] Creanor SL, Russell JI, Strang DM, et al. The prevalence of clinically undetected occlusal dentine caries in Scottish adolescents[J]. Br Dent J, 1990,169(5):126-129.
[91] Wenzel A, Fejerskov O. Validity of diagnosis of questionable caries lesions in occlusal surfaces of extracted third molars[J]. Caries Res, 1992,26(3):188-194.
[92] Schneiderman A, Elbaum M, Shultz T, et al. Assessment of dental caries with Digital Imaging Fiber-Optic TransIllumination (DIFOTI): in vitro study[J]. Caries Res, 1997,31(2):103-110.
[93] Lussi A, Firestone A, Schoenberg V, et al. In vivo diagnosis of fissure caries using a new electrical resistance monitor[J]. Caries Res, 1995,29(2):81-87.
[94] Thylstrup A, Bille J, Qvist V. Radiographic and observed tissue changes in approximal carious lesions at the time of operative treatment[J]. Caries Res, 1986,20(1):75-84.
[95] Reynolds EC, Johnson IH. Effect of milk on caries incidence and bacterial composition of dental plaque in the rat[J]. Arch Oral Biol, 1981,26(5):445-451.
[96] Rosen S, Min DB, Harper DS, et al. Effect of cheese, with and without sucrose, on dental caries and recovery of Streptococcus mutans in rats[J]. J Dent Res, 1984,63(6):894-896.
[97] Harper DS, Osborn JC, Hefferren JJ, et al. Cariostatic evaluation of cheeses with diverse physical and compositional characteristics[J]. Caries Res, 1986,20(2):123-130.
[98] Harper DS, Osborn JC, Clayton R, et al. Modification of food cariogenicity in rats by mineral-rich concentrates from milk[J]. J Dent Res, 1987,66(1):42-45.
[99] Krobicka A, Bowen WH, Pearson S, et al. The effects of cheese snacks on caries in desalivated rats[J]. J Dent Res, 1987,66(6):1116-1119.
[100] Silva MF, Burgess RC, Sandham HJ, et al. Effects of water-soluble components of cheese on experimental caries in humans[J]. J Dent Res, 1987,66(1):38-41.
[101] Reynolds EC, del Rio A. Effect of casein and whey-protein solutions on caries experience and feeding patterns of the rat[J]. Arch Oral Biol, 1984,29(11):927-933.
[102] Reynolds EC, Black CL. Confectionery composition and rat caries[J].Caries Res, 1987,21(6):538-545.
[103] Reynolds EC, Black CL. Reduction of cholate's cariogenicity by supplementation with sodium caseinate[J]. Caries Res, 1987,21(5):445-451.
[104] Reynolds EC. The prevention of sub-surface demineralization of bovine enamel and change in plaque composition by casein in an intra-oral model[J]. J Dent Res, 1987,66(6):1120-1127.
[105] Reynolds EC, Black CL. Cariogenicity of a confection supplemented with sodium caseinate at a palatable level[J]. Caries Res, 1989,23(5):368-370.
[106] Shaw JH. Effects of dietary composition on tooth decay in the albino rat[J]. J Nutr, 1950,41(1):13-24.
[107] Bavetta LA, Mc CF. Protein factors and experimental rat caries[J]. J Nutr, 1957,63(1):107-117.
[108] Murray MJ, Snowden MJ. The preparation, characterization and applications of colloidal microgels.[J]. Advance in Colloid and Interface Science, 1995,54:73-91.
[109] Boggs LJ, M. R, Bike SG. Characterization and rheological investigation of polymer microgels used in automotive coatings[J]. Journal of Coating Technology, 1996,68(855):63-74.
[110] Sasa N, Yamaoka T. Surface-activated photopolymer microgels.[J]. Advanced Materials, 1994,6(5):417-421.
[111] Bergbreiter DE, Case BL, Liu YS, et al. Poly(N-isopeopylacrylamide) soluble polymer supports in catalysis and synthsis.[J]. macromolecules, 1998,31(18):6053-6062.
[112] Arndt KF, Kucklin D, Richter A. Application of sensitive hydrogels in flow control.[J]. Polymers for Advanced Technologies, 2000,11(12):496-505.
[113] Bawendi MG, Carroll PJ, Wilson WL, et al. Luminescence properties of CdSe quantum crystallites-resonance between interior and surface localized states.[J]. Journal of Chemical Physics, 1992,96(2):946-954.
[114] Antonietti M, Grohn F, Hartmann J, et al. Nonclassical shapes ofnoble-metal colloids by synthesis in microgel nanoreactors.[J]. Augewandte Chemie-Inernational Edition In English, 1997,36(19):2080-2083.
[115] Blanco A, Lopez C, Mayoral R, et al. CdS photoluminescence inhibition by a photonic structure.[J]. Applied Physics Letters, 1998,73(13):1781-1783.
[116]汪玉松,邹思湘.乳生物化学[M].吉林:吉林大学出版社, 1995.
[117]张和平,张列兵.现代乳品手册[M].北京:中国轻工业出版社, 2005.
[118] Xu SQ, Zhang JG, Paquet G, et al. From hybrid microgels to photonic crystals.[J]. Advance Functional Materials, 2003,13(6):468-472.
[119] Elaissari A, Ganachaud F, Pichot C. Biorelevant latexes and microgels for the interaction with nucleic acids.[J]. Colloid Chemistry, 2003,227:169-193.
[120] Chen LY, Pemondetto GE, Subirade M. Food protein-based materials as nurraceutical delivery systems.[J]. Trends in Food Science & Technology, 2006,17(5):272-283.
[121] McGann TC, Kearney RD, Buchheim W, et al. Amorphous calcium phosphate in casein micelles of bovine milk[J]. Calcif Tissue Int, 1983,35(6):821-823.
[122] McGann TC, Buchheim W, Kearney RD, et al. Composition and ultrastructure of calcium phosphate-citrate complexes in bovine milk systems[J]. Biochim Biophys Acta, 1983,760(3):415-420.
[123] Holt C, Wahlgren NM, Drakenberg T. Ability of a beta-casein phosphopeptide to modulate the precipitation of calcium phosphate by forming amorphous dicalcium phosphate nanoclusters[J]. Biochem J, 1996,314 ( Pt 3):1035-1039.
[124] Reynolds EC, Black CL, Cai F, et al. Advances in enamel remineralization: casein phosphopeptide-amorphous calcium phosphate. [J]. J Clin Dent, 1999(10):86-88.
[125] Ametani A, Kaminogawa S, Shimizu M, et al. Rapid screening of antigenically reactive fragments of alpha s1-casein using HPLC andELISA[J]. J Biochem, 1987,102(2):421-425.
[126] Meisel H, Frister H. Chemical characterization of a caseinophosphopeptide isolated from in vivo digests of a casein diet[J]. Biol Chem Hoppe Seyler, 1988,369(12):1275-1279.
[127] Reynolds EC, Riley PF, Adamson NJ. A selective precipitation purification procedure for multiple phosphoseryl-containing peptides and methods for their identification[J]. Anal Biochem, 1994,217(2):277-284.
[128] Cross KJ, Huq NL, Bicknell W, et al. Cation-dependent structural features of beta-casein-(1-25)[J]. Biochem J, 2001,356(1):277-286.
[129] Huq NL, Cross KJ, Reynolds EC. A 1H NMR study of the casein phosphopeptideαS2-casein(1-21)[J]. J Dent Res, 1995,74:579.
[130] Huq NL, Cross KJ, Reynolds EC. A 1H-NMR study of the casein phosphopeptide alpha s1-casein(59-79)[J]. Biochim Biophys Acta, 1995,1247(2):201-208.
[131] Ferraretto A, Gravaghi C, Fiorilli A, et al. Casein-derived bioactive phosphopeptides: role of phosphorylation and primary structure in promoting calcium uptake by HT-29 tumor cells[J]. FEBS Lett, 2003,551(1-3):92-98.
[132] Cross KJ, Huq NL, Reynolds EC. Casein phosphopeptides in oral health--chemistry and clinical applications[J]. Curr Pharm Des, 2007,13(8):793-800.
[133] Marsh ME. Binding of calcium and phosphate ions to dentin phosphophoryn[J]. Biochemistry, 1989,28(1):346-352.
[134] Green MR, Pastewka JV, Peacock AC. Differential staining of phosphoproteins on polyacrylamide gels with a cationic carbocyanine dye[J]. Anal Biochem, 1973,56(1):43-51.
[135] Cross KJ, Huq NL, Reynolds E, C,. Anticariogenic peptides. In: Nutraceutical proteins and peptides in health and disease[J]. CRC Press, 2005:335-351.
[136] Naito H. CPP: intro-intestenal formation and significance in thebioavailability of calcium.[J]. Japan Sci Press, 1990:207-220.
[137] Reeves RE, Latour NG. Calcium phosphate sequestering phosphopeptide from casein[J]. Science, 1958,128(3322):472.
[138] Reynolds EC. Remineralization of enamel subsurface lesions by casein phosphopeptide-stabilized calcium phosphate solutions[J]. J Dent Res, 1997,76(9):1587-1595.
[139] van Dijk JW, Borggreven JM, Driessens FC. Diffusion in mammalian tooth enamel in relation to the caries process[J]. Arch Oral Biol, 1983,28(7):591-597.
[140] Borggreven JM, van Dijk JW, Driessens FC. Effect of mono- and divalent ions on diffusion and binding in bovine tooth enamel[J]. Arch Oral Biol, 1981,26(8):663-669.
[141] Cross KJ, Huq NL, Stanton DP, et al. NMR studies of a novel calcium, phosphate and fluoride delivery vehicle-alpha(S1)-casein(59-79) by stabilized amorphous calcium fluoride phosphate nanocomplexes[J]. Biomaterials, 2004,25(20):5061-5069.
[142] ten Cate JM, van Loveren C. Fluoride mechanisms[J]. Dent Clin North Am, 1999,43(4):713-742, vii.
[143] Cochrane NJ, Saranathan S, Cai F, et al. Enamel subsurface lesion remineralisation with casein phosphopeptide stabilised solutions of calcium, phosphate and fluoride[J]. Caries Res, 2008,42(2):88-97.
[144] Gray JA. Kinetics of enamel dissolution during formation of incipient caries-like lesions[J]. Arch Oral Biol, 1966,11(4):397-422.
[145] Holly FJ, Gray JA. Mechanism for incipient carious lesion growth utilizing a physical model based on diffusion concepts[J]. Arch Oral Biol, 1968,13(3):319-334.
[146] Featherstone JD, Rodgers BE, Smith MW. Physicochemical requirements for rapid remineralization of early carious lesions[J]. Caries Res, 1981,15(3):221-235.
[147] Reynolds EC, Cai F, Shen P, et al. Retention in plaque andremineralization of enamel lesions by various forms of calcium in a mouthrinse or sugar-free chewing gum[J]. J Dent Res, 2003,82(3):206-211.
[148] Buchalla W, Attin T, Schulte-Monting J, et al. Fluoride uptake, retention, and remineralization efficacy of a highly concentrated fluoride solution on enamel lesions in situ[J]. J Dent Res, 2002,81(5):329-333.
[149] O'Reilly MM, Featherstone JD. Demineralization and remineralization around orthodontic appliances: an in vivo study[J]. Am J Orthod Dentofacial Orthop, 1987,92(1):33-40.
[150] Ogaard B, Rolla G, Arends J. Orthodontic appliances and enamel demineralization. Part 1. Lesion development[J]. Am J Orthod Dentofacial Orthop, 1988,94(1):68-73.
[151] Glatz EGM, Featherstone JDB. Demineralization related to orthodontic bands and brackets-clinical study[J]. Am J Orthod Dentofacial Orthop, 1985,1985:87.
[152]王翰章.中华口腔科学(上卷)[M].北京:人民卫生出版社, 2001.
[153] Mattingly JA, Sauer GJ, Yancey JM, et al. Enhancement of Streptococcus mutans colonization by direct bonded orthodontic appliances[J]. J Dent Res, 1983,62(12):1209-1211.
[154]陈梅红,李璞,尚斌.正畸与正常儿童龋活性比较[J].口腔医学纵横, 2002,18(1):28-29.
[155] Jordan C, LeBlanc DJ. Influences of orthodontic appliances on oral populations of mutans streptococci[J]. Oral Microbiol Immunol, 2002,17(2):65-71.
[156] Chang HS, Walsh LJ, Freer TJ. The effect of orthodontic treatment on salivary flow, pH, buffer capacity, and levels of mutans streptococci and lactobacilli[J]. Aust Orthod J, 1999,15(4):229-234.
[157]潘一春,张丁,傅民魁.口腔正畸学[M].北京:人民卫生出版社, 2003.
[158]王翰章.中华口腔科学(中卷)[M].北京:人民卫生出版社, 2001.
[159] Geiger AM, Gorelick L, Gwinnett AJ, et al. The effect of a fluorideprogram on white spot formation during orthodontic treatment[J]. Am J Orthod Dentofacial Orthop, 1988,93(1):29-37.
[160] Zimmer BW, Rottwinkel Y. Assessing patient-specific decalcification risk in fixed orthodontic treatment and its impact on prophylactic procedures[J]. Am J Orthod Dentofacial Orthop, 2004,126(3):318-324.
[161] Sonis AL, Snell W. An evaluation of a fluoride-releasing, visible light-activated bonding system for orthodontic bracket placement[J]. Am J Orthod Dentofacial Orthop, 1989,95(4):306-311.
[162] Hobson RS, Clark JD. How UK orthodontists advise patients on oral hygiene[J]. Br J Orthod, 1998,25(1):64-66.
[163] Lees A, Rock WP. A comparison between written, verbal, and videotape oral hygiene instruction for patients with fixed appliances[J]. J Orthod, 2000,27(4):323-328.
[164] Zimmer B. Systematic decalcification prophylaxis during treatment with fixed appliances[J]. J Orofac Orthop, 1999,60(3):205-214.
[165] Sengun A, Sari Z, Ramoglu SI, et al. Evaluation of the dental plaque pH recovery effect of a xylitol lozenge on patients with fixed orthodontic appliances[J]. Angle Orthod, 2004,74(2):240-244.
[166] Mattick CR, Mitchell L, Chadwick SM, et al. Fluoride-releasing elastomeric modules reduce decalcification: a randomized controlled trial[J]. J Orthod, 2001,28(3):217-219.
[167] Doherty UB, Benson PE, Higham SM. Fluoride-releasing elastomeric ligatures assessed with the in situ caries model[J]. Eur J Orthod, 2002,24(4):371-378.
[168] Trent JM, Bittner M, Zhang J, et al. Use of microgenomic technology for analysis of alterations in DNA copy number and gene expression in malignant melanoma[J]. Clin Exp Immunol, 1997,107 Suppl 1:33-40.
[169] Rawls HR, Zimmerman BF. Fluoride-exchanging resins for caries protection[J]. Caries Res, 1983,17(1):32-43.
[170] Chung CK, Millett DT, Creanor SL, et al. Fluoride release and cariostaticability of a compomer and a resin-modified glass ionomer cement used for orthodontic bonding[J]. J Dent, 1998,26(5-6):533-538.
[171] Capilouto ML, DePaola PF, Gron P. In vivo study of slow-release fluoride resin and enamel uptake[J]. Caries Res, 1990,24(6):441-445.
[172] Gorton J, Featherstone JD. In vivo inhibition of demineralization around orthodontic brackets[J]. Am J Orthod Dentofacial Orthop, 2003,123(1):10-14.
[173] Glasspoole EA, Erickson RL, Davidson CL. Demineralization of enamel in relation to the fluoride release of materials[J]. Am J Dent, 2001,14(1):8-12.
[174] Corry A, Millett DT, Creanor SL, et al. Effect of fluoride exposure on cariostatic potential of orthodontic bonding agents: an in vitro evaluation[J]. J Orthod, 2003,30(4):323-329.
[175]胡炜,王勤,傅民魁.口腔正畸固定矫治器应用中釉质脱矿的临床调查[J].口腔正畸学, 2001,8(2):51-54.
[176] Attin T, Knofel S, Buchalla W, et al. In situ evaluation of different remineralization periods to decrease brushing abrasion of demineralized enamel[J]. Caries Res, 2001,35(3):216-222.
[177] Marinelli CB, Donly KJ, Wefel JS, et al. An in vitro comparison of three fluoride regimens on enamel remineralization[J]. Caries Res, 1997,31(6):418-422.
[178] Eakle WS, Featherstone JD, Weintraub JA, et al. Salivary fluoride levels following application of fluoride varnish or fluoride rinse[J]. Community Dent Oral Epidemiol, 2004,32(6):462-469.
[179] Yamamoto H, Ooya K. Potential of yttrium-aluminum-garnet laser in caries prevention[J]. J Oral Pathol, 1974,3(1):7-15.
[180] Bahar A, Tagomori S. The effect of normal pulsed Nd-YAG laser irradiation on pits and fissures in human teeth[J]. Caries Res, 1994,28(6):460-467.
[181] Forsberg CM, Oliveby A, Lagerlof F. Salivary clearance of sugar beforeand after insertion of fixed orthodontic appliances[J]. Am J Orthod Dentofacial Orthop, 1992,102(6):527-530.
[182] Gorelick L, Geiger AM, Gwinnett AJ. Incidence of white spot formation after bonding and banding[J]. Am J Orthod, 1982,81(2):93-98.
[183] Mitchell L. Decalcification during orthodontic treatment with fixed appliances--an overview[J]. Br J Orthod, 1992,19(3):199-205.
[184] Yoshihara A, Sakuma S, Kobayashi S, et al. Antimicrobial effect of fluoride mouthrinse on mutans streptococci and lactobacilli in saliva[J]. Pediatr Dent, 2001,23(2):113-117.
[185] Willmot DR. White lesions after orthodontic treatment: does low fluoride make a difference?[J]. J Orthod, 2004,31(3):235-242.
[186] Linton JL. Quantitative measurements of remineralization of incipient caries[J]. Am J Orthod Dentofacial Orthop, 1996,110(6):590-597.
[187] Chang HS, Walsh LJ, Freer TJ. Enamel demineralization during orthodontic treatment. Aetiology and prevention[J]. Aust Dent J, 1997,42(5):322-327.
[188] Lagerweij MD, Damen JJM, Stookey GK. Remineralization of small lesions by ?uoride. [J]. J Dent Res 1997,76(Spec Issue):16.
[189] Brostek AM, Bochenek AJ, Walsh LJ. Minimally invasive dentistry: a review and update[J]. Shanghai Kou Qiang Yi Xue, 2006,15(3):225-249.
[190] Rose RK. Effects of an anticariogenic casein phosphopeptide on calcium diffusion in streptococcal model dental plaques[J]. Arch Oral Biol, 2000,45(7):569-575.
[191] Iijima Y, Cai F, Shen P, et al. Acid resistance of enamel subsurface lesions remineralized by a sugar-free chewing gum containing casein phosphopeptide-amorphous calcium phosphate[J]. Caries Res, 2004,38(6):551-556.
[192] Pourghadiri M, Longhurst P, Watson TF. A new technique for the controlled removal of mottled enamel: measurement of enamel loss[J]. Br Dent J, 1998,184(5):239-241.
[193] Welbury RR, Carter NE. The hydrochloric acid-pumice microabrasion technique in the treatment of post-orthodontic decalcification[J]. Br J Orthod, 1993,20(3):181-185.
[194] Croll TP, Cavanaugh RR. Enamel color modification by controlled hydrochloric acid-pumice abrasion. I. technique and examples[J]. Quintessence Int, 1986,17(2):81-87.
[195] Murphy TC, Willmot DR, Rodd HD. Management of postorthodontic demineralized white lesions with microabrasion: a quantitative assessment[J]. Am J Orthod Dentofacial Orthop, 2007,131(1):27-33.
[196] Kendall RL. Hydrochloric acid removal of brown fluorosis stains: clinical and scanning electron micrographic observations.[J]. Quintessence Int, 1990,20(837-839)
[197] Jacobsson-Hunt U, M.R. H. The effect of hydrochloric acid pumice abrasion on fluorosed teeth.[J]. J Dent Res, 1989,86:982.
[198] Peariasamy K, Anderson P, Brook AH. A quantitative study of the effect of pumicing and etching on the remineralization of enamel opacities.[J]. Int J Pediatr Dent, 2001,11:193-200.
[199] Ardu S, Castioni NV, Benbachir N, et al. Minimally invasive treatment of white spot enamel lesions[J]. Quintessence Int, 2007,38(8):633-636.
[200] Reddy MB, Love M. The impact of food processing on the nutritional quality of vitamins and minerals[J]. Adv Exp Med Biol, 1999,459:99-106.
[201]王芸,唐荣银.五倍子水提取物处理早期人工釉质龋后表面结构的扫描电镜观察[J].牙体牙髓牙周病学杂志, 2005,15(3):133-135.
[202]戴啊师,蔡春木,海蓝.天然药物隔山消促进釉质再矿化的研究[J].牙体牙髓牙周病学杂志, 2007,17(11):635-637.
[203]吕奎龙,滕立群.纳米羟基磷灰石牙膏对人工龋再矿化研究[J].黑龙江医药科学, 2002,25:26-27.
[204] Sculean A, Windisch P, Keglevich T, et al. Clinical and histologic evaluation of human intrabony defects treated with an enamel matrix protein derivative combined with a bovine-derived xenograft[J]. Int JPeriodontics Restorative Dent, 2003,23(1):47-55.
[205]王志伟,赵月萍,周长忍, et al.牙釉基质蛋白诱导牙釉质的再矿化研究[J].四川大学学报(医学版), 2008,39(4):579-582.
[206]刘洪涛,葛世荣,朱华.微粒团聚的尺度效应及其分形表征[J].润滑与密封, 2006,184:1-3.
[207]陈洪云,金斌,董英杰等.测定钛白粉平均粒径的研究[J].涂料工业, 2002(6):40-41.
[208]王振新,钱彦虎.激光粒度仪测定PTA粒径分布[J].聚酯工业, 2004(3):50-53.
[209] Holt C, Timmins PA, Errington N, et al. A core-shell model of calcium phosphate nanoclusters stabilized by beta-casein phosphopeptides, derived from sedimentation equilibrium and small-angle X-ray and neutron-scattering measurements[J]. Eur J Biochem, 1998,252(1):73-78.
[210] Hung CH, Hsung MN. Dispersion and stabilization of aqueous TiO 2 suspension.[J]. Ceramics International, 1995(21):65-68.
[211] Brown LR, White JO, Horton IM, et al. Effect of continuous fluoride gel use on plaque fluoride retention and microbial activity[J]. J Dent Res, 1983,62(6):746-751.
[212]盛江筠,刘正.变形链球菌耐氟菌株的诱导及产酸性的实验研究[J].中华口腔医学杂志, 2000,35(2):95-98.
[213] Vacca-Smith AM, Bowen WH. The effect of milk and kappa casein on streptococcal glucosyltransferase[J]. Caries Res, 1995,29(6):498-506.
[214] Schupbach P, Neeser JR, Golliard M, et al. Incorporation of caseinoglycomacropeptide and caseinophosphopeptide into the salivary pellicle inhibits adherence of mutans streptococci[J]. J Dent Res, 1996,75(10):1779-1788.
[215]刘兴容,徐皑李,李艳萍等.酪蛋白磷酸肽钙磷复合体对变形链球菌产酸影响的实验研究[J].现代口腔医学杂志, 2007,21(1):63-65.
[216] Nishioka Y, Nakagaki H, Kato S, et al. Fluoride profiles in different sites of approximal surfaces of second primary molars after topical application ofacidulated phosphate fluoride gel in vivo[J]. Arch Oral Biol, 1995,40(12):1157-1161.
[217] Riordan PJ. Dental fluorosis, dental caries and fluoride exposure among 7-year-olds[J]. Caries Res, 1993,27(1):71-77.
[218] Koch G, Bergmann-Arnadottir I, Bjarnason S, et al. Caries-preventive effect of fluoride dentifrices with and without anticalculus agents: a 3-year controlled clinical trial[J]. Caries Res, 1990,24(1):72-79.
[219] Pine CM, ten Bosch JJ. Dynamics of and diagnostic methods for detecting small carious lesions[J]. Caries Res, 1996,30(6):381-388.
[220] Mallon DE, Mellberg JR. Analysis of dental hard tissue by computerized microdensitometry[J]. J Dent Res, 1985,64(2):112-116.
[221]刘艳辉.显微放射摄影术在口腔科的应用[J].上海医学, 2001,24(增刊):66-68.
[222] Fontana M, Li Y, Dunipace AJ, et al. Measurement of enamel demineralization using microradiography and confocal microscopy. A correlation study[J]. Caries Res, 1996,30(5):317-325.
[223] Iijima Y, Takagi O, Duschner H, et al. Influence of nail varnish on the remineralization of enamel single sections assessed by microradiography and confocal laser scanning microscopy[J]. Caries Res, 1998,32(5):393-400.
[224]叶应妩,王毓三.全国临床检验操作规程[M].江苏:东南大学出版社, 1997.
[225]马美范,王介峰.用硫酸亚铁铵作还原剂测定食品中磷含量[J].广西轻工业, 1998(1):43-45.
[226] Reynolds EC. Anticariogenic complexes of amorphous calcium phosphate stabilized by casein phosphopeptides: a review[J]. Spec Care Dentist, 1998,18(1):8-16.
[227] Mazzaoui SA, Burrow MF, Tyas MJ, et al. Incorporation of casein phosphopeptide-amorphous calcium phosphate into a glass-ionomer cement[J]. J Dent Res, 2003,82(11):914-918.
[228]赵琼芝,蔡璠.酪蛋白磷酸多肽钙氟磷复合体对牙釉质缺损再矿化的实验研究[J].中华口腔医学杂志, 2001,36 (6):421-423.
[229] Arends J, Christoffersen J. Nature and role of loosely bound fluoride in dental caries[J]. J Dent Res, 1990,69 Spec No:601-605; discussion 634-606.
[230] Reynolds EC, Cai F, Cochrane NJ, et al. Fluoride and casein phosphopeptide-amorphous calcium phosphate[J]. J Dent Res, 2008,87(4):344-348.
[231] Tucker K, Adams M, Shaw L, et al. Human enamel as a substrate for in vitro acid dissolution studies: influence of tooth surface and morphology[J]. Caries Res, 1998,32(2):135-140.
[232] Kirkham J, Robinson C, Strong M, et al. Effects of frequency and duration of acid exposure on demineralization/remineralization behaviour of human enamel in vitro[J]. Caries Res, 1994,28(1):9-13.
[233] Meurman JH, Frank RM. Progression and surface ultrastructure of in vitro caused erosive lesions in human and bovine enamel[J]. Caries Res, 1991,25(2):81-87.
[234] Shellis RP. A scanning electron-microscopic study of solubility variations in human enamel and dentine[J]. Arch Oral Biol, 1996,41(5):473-484.
[235] Larsen MJ, Richards A. The influence of saliva on the formation of calcium fluoride-like material on human dental enamel[J]. Caries Res, 2001,35(1):57-60.
[236]吴红崑,周学东,谭红.微量元素矿化液促进釉质龋再矿化层形成机理的研究[J].华西口腔医学杂志, 2000,18(4):219-221.
[237] Damato FA, Strang R, Stephen KW. Effect of fluoride concentration on remineralization of carious enamel: an in vitro pH-cycling study[J]. Caries Res, 1990,24(3):174-180.
[238]陈向群,王勤.氟化钠和再矿化液对早期釉质龋作用的电子探针和显微硬度研究[J].北京医科大学学报, 1995,27(4):287.
[239] Craig GG. The use of a calcium sucrose phosphates-calciumorthophosphate complex as a cariostatic agent[J]. Br Dent J, 1975,138(1):25-28.
[240] Prescott B, Renugopalakrishnan V, Glimcher MJ, et al. A Raman spectroscopic study of hen egg yolk phosvitin: structures in solution and in the solid state[J]. Biochemistry, 1986,25(10):2792-2798.
[241] Crossley M, Huq NL, Kirszbaum L, et al. Purification and N-terminal sequence analysis of bovine dentine phosphophoryn[J]. J Dent Res, 1996,75(spec Iss):154.
[242] Sorensen ES, Petersen TE. Identification of two phosphorylation motifs in bovine osteopontin[J]. Biochem Biophys Res Commun, 1994,198(1):200-205.
[243] Binning G, Quate CF, Gerber C. Atomic force microscope[J]. J Phys Rev Lett, 1986,56(9):930-933.
[244]宗仁鹤,白春礼,李振刚, et al.原子力显微镜及其在激光作用DNA的结构研究中的作用[J].量子电子学, 1996,13(2):107-112.
[245]陈宝安,杜鹃.原子力显微镜在临床医学中的应用[J].现代医学仪器与应用, 2003,15(4):7-10.
[246]张英鸽,饶曼人.原子力显微技术的基本原理及药理学应用[J].药学学报, 2000,35(6):476-480.
[247] Chang L, Kious T, Yorgancioglu M, et al. Cytoskeleton of living, unstained cells imaged by scanning force microscopy[J]. Biophys J, 1993,64(4):1282-1286.
[248] Kasas S, Gotzos V, Celio MR. Observation of living cells using the atomic force microscope[J]. Biophys J, 1993,64(2):539-544.
[249] Radmacher M, Tillamnn RW, Fritz M, et al. From molecules to cells: imaging soft samples with the atomic force microscope[J]. Science, 1992,257(5078):1900-1905.
[250] Hoh JH, Lal R, John SA, et al. Atomic force microscopy and dissection of gap junctions[J]. Science, 1991,253(5026):1405-1408.
[251] Butt HJ, Downing KH, Hansma PK. Imaging the membrane proteinbacteriorhodopsin with the atomic force microscope[J]. Biophys J, 1990,58(6):1473-1480.
[252] Soon LL, Bottema C, Breed WG. Atomic force microscopy and cytochemistry of chromatin from marsupial spermatozoa with special reference to Sminthopsis crassicaudata[J]. Mol Reprod Dev, 1997,48(3):367-374.
[253] Lal R. Imaging molecular structure of channels and receptors with an atomic force microscope[J]. Scanning Microsc Suppl, 1996,10:81-95; discussion 95-86.
[254] Arakawa H, Umemura K, Ikai A. Protein images obtained by STM, AFM and TEM[J]. Nature, 1992,358(6382):171-173.
[255] Yip CM, Yip CC, Ward MD. Direct force measurements of insulin monomer-monomer interactions[J]. Biochemistry, 1998,37(16):5439-5449.
[256] Merkel R, Nassoy P, Leung A, et al. Energy landscapes of receptor-ligand bonds explored with dynamic force spectroscopy[J]. Nature, 1999,397(6714):50-53.
[257] Hoyt PR, Doktycz MJ, Warmack RJ, et al. Spin-column isolation of DNA-protein interactions from complex protein mixtures for AFM imaging[J]. Ultramicroscopy, 2001,86(1-2):139-143.
[258] Jiao Y, Cherny DI, Heim G, et al. Dynamic interactions of p53 with DNA in solution by time-lapse atomic force microscopy[J]. J Mol Biol, 2001,314(2):233-243.
[259] Dammer U, Hegner M, Anselmetti D, et al. Specific antigen/antibody interactions measured by force microscopy[J]. Biophys J, 1996,70(5):2437-2441.
[260] Willemsen OH, Snel MM, Cambi A, et al. Biomolecular interactions measured by atomic force microscopy[J]. Biophys J, 2000,79(6):3267-3281.
[261] Hinterdorfer P, Baumgartner W, Gruber HJ, et al. Detection and localization of individual antibody-antigen recognition events by atomicforce microscopy[J]. Proc Natl Acad Sci U S A, 1996,93(8):3477-3481.
[262] Willemsen OH, Snel MM, van der Werf KO, et al. Simultaneous height and adhesion imaging of antibody-antigen interactions by atomic force microscopy[J]. Biophys J, 1998,75(5):2220-2228.
[263] Schwesinger F, Ros R, Strunz T, et al. Unbinding forces of single antibody-antigen complexes correlate with their thermal dissociation rates[J]. Proc Natl Acad Sci U S A, 2000,97(18):9972-9977.
[264] Smulson ME, Pang D, Jung M, et al. Irreversible binding of poly(ADP)ribose polymerase cleavage product to DNA ends revealed by atomic force microscopy: possible role in apoptosis[J]. Cancer Res, 1998,58(16):3495-3498.
[265] Ellis DJ, Dryden DT, Berge T, et al. Direct observation of DNA translocation and cleavage by the EcoKI endonuclease using atomic force microscopy[J]. Nat Struct Biol, 1999,6(1):15-17.
[266] Valle M, Valpuesta JM, Carrascosa JL, et al. The interaction of DNA with bacteriophage phi 29 connector: a study by AFM and TEM[J]. J Struct Biol, 1996,116(3):390-398.
[267] Habelitz S, Balooch M, Marshall SJ, et al. In situ atomic force microscopy of partially demineralized human dentin collagen fibrils[J]. J Struct Biol, 2002,138(3):227-236.
[268] Habelitz S, Marshall SJ, Marshall GW, Jr., et al. The functional width of the dentino-enamel junction determined by AFM-based nanoscratching[J]. J Struct Biol, 2001,135(3):294-301.
[269] Hegedus C, Bistey T, Flora-Nagy E, et al. An atomic force microscopy study on the effect of bleaching agents on enamel surface[J]. J Dent, 1999,27(7):509-515.
[270] ten Cate JM, Imfeld T. Dental erosion, summary[J]. Eur J Oral Sci, 1996,104(2):241-244.
[271] Zachrisson BJ. A posttreatment evaluation of direct bonding in orthodontics[J]. Am J Orthod, 1977,71(2):173-189.
[272] Sudjalim TR, Woods MG, Manton DJ, et al. Prevention of demineralization around orthodontic brackets in vitro[J]. Am J Orthod Dentofacial Orthop, 2007,131(6):701-709.
[273] Mizrahi E. Enamel demineralization following orthodontic treatment[J]. Am J Orthod, 1982,82(1):62-67.
[274] von der FF. The effect of fluorides on the caries resistance of enamel[J]. Acta Odontol Scand, 1961,19:431-442.
[275] Downer MC. Caries prevalence in the United Kingdom[J]. Int Dent J, 1994,44(4 Suppl 1):365-370.
[276] Marcusson A, Norevall LI, Persson M. White spot reduction when using glass ionomer cement for bonding in orthodontics: a longitudinal and comparative study[J]. Eur J Orthod, 1997,19(3):233-242.
[277] Derrick W. White Spot Lesions After Orthodontic Treatment[J]. Seminars in Orthodontics, 2008,14(3):209-219.
[278] Banks PA, Richmond S. Enamel sealants: a clinical evaluation of their value during fixed appliance therapy[J]. Eur J Orthod, 1994,16(1):19-25.
[279] Mizrahi E. Surface distribution of enamel opacities following orthodontic treatment[J]. Am J Orthod, 1983,84(4):323-331.
[280] Benson PE, Pender N, Higham SM, et al. Morphometric assessment of enamel demineralisation from photographs[J]. J Dent, 1998,26(8):669-677.
[281] Benson PE, Pender N, Higham SM. Enamel demineralisation assessed by computerised image analysis of clinical photographs[J]. J Dent, 2000,28(5):319-326.
[282] Willmot DR, Benson PE, Pender N, et al. Reproducibility of quantitative measurement of white enamel demineralisation by image analysis[J]. Caries Res, 2000,34(2):175-181.
[283] Christoffersen J, christoffersen MR, Johansen T. Kinetics of growth and dissolution of fluorapatite[J]. J Cryst Growth, 1996,163:295-303.