雷竹不同变异类型的遗传多样性分析
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摘要
雷竹(Phyllostachys violascens)是优良笋用经济竹种,经过长期的自然演化与人工栽培,种内产生了一定程度的遗传变异,形成了若干变异类型。本文从形态特征、出笋-成竹规律、DNA遗传变异等方面对雷竹16个变异类型进行研究,取得如下的研究结果:
1、雷竹16个变异类型的8个表型性状的平均值、方差、标准差、变异系数等基本统计分析表明,雷竹不同变异类型间表型性状的遗传变异较大,变异系数为10.1%-41.4%之间,其中枝下高的变异最大,胸径和株高次之。通过表型性状的聚类分析可以将雷竹分成5类。
2、通过研究雷竹16个变异类型的出笋-成竹规律,结果表明:弯秆雷竹、红壳雷竹等变异类型笋期较早,而雷山乌等较晚;不同变异类型的单株立竹出笋量、成竹率等差异显著。
3、用AFLP、SRAP和ACGM标记分析雷竹16个变异类型的遗传多样性。结果:15对AFLP引物、15对SRAP引物和38个ACGM引物分别扩增出253、152和75个多态性位点。对3种标记结果进行UPGMA聚类分析,结果极其相似,两两之间呈极显著正相关。分子标记结果表明:(1)雷竹不同变异类型间存在丰富的遗传变异;(2)根据UPGMA聚类可以将雷竹不同变异类型分为4类。
Phyllostachys violascens is an execellent economic bamboo species in China, which had many variations types by the long natural evolution and artificial cultivation.The morphological characteristics, rhythm of bamboo shooting and DNA genetic variation of 16 variation types of Ph.violascens were studied. The major results were as follows:
1, Such basic statistical analysis as the mean, variance, standard deviation, CV(coefficient of variation)to the morphological characters of 16 variation types of Ph.violascens indicated that great diversity existed among different variation types, with ranging from 10.1%-41.4%. The height under branch has the highest variation,Diameter at breast height and The plant height was followed. The variation types of Ph.violascens were divided into 5 clusters by morphological characteristics, and which together clusters with similar morphological characteristics.
2, Based on the surveys of bamboo shoots growth of 16 variation types of Ph.violascens, the results show that Ph.violascens cv. Wanggan, Ph.violascens cv. Hongke emerge shoots earlier than Ph.violascens cv. Atrovaginis. Variation of the emerging shoots rates and grown bamboo rates are remarkable in different variation types.
3, Genetic diversity of 16 variation types of Ph.violascens was evaluated using AFLP, SRAP and ACGM markers. 15 AFLP primer pairs, 15 SRAP primer pairs and 38 ACGM primers generated 253, 152 and 75 polymorphic bands, respectively. These 3 molecular markers had consistent result from the clustering analysis by UPGMA, the significant correlation between AFLP, SRAP, and ACGM markers was observed. UPGMA method cluster analysis showed: (1) There are abundant genetic variation among different variation types of Ph.violascens; (2) The variation types of Ph.violascens were divided into 4 clusters by UPGMA.
1、雷竹16个变异类型的8个表型性状的平均值、方差、标准差、变异系数等基本统计分析表明,雷竹不同变异类型间表型性状的遗传变异较大,变异系数为10.1%-41.4%之间,其中枝下高的变异最大,胸径和株高次之。通过表型性状的聚类分析可以将雷竹分成5类。
2、通过研究雷竹16个变异类型的出笋-成竹规律,结果表明:弯秆雷竹、红壳雷竹等变异类型笋期较早,而雷山乌等较晚;不同变异类型的单株立竹出笋量、成竹率等差异显著。
3、用AFLP、SRAP和ACGM标记分析雷竹16个变异类型的遗传多样性。结果:15对AFLP引物、15对SRAP引物和38个ACGM引物分别扩增出253、152和75个多态性位点。对3种标记结果进行UPGMA聚类分析,结果极其相似,两两之间呈极显著正相关。分子标记结果表明:(1)雷竹不同变异类型间存在丰富的遗传变异;(2)根据UPGMA聚类可以将雷竹不同变异类型分为4类。
Phyllostachys violascens is an execellent economic bamboo species in China, which had many variations types by the long natural evolution and artificial cultivation.The morphological characteristics, rhythm of bamboo shooting and DNA genetic variation of 16 variation types of Ph.violascens were studied. The major results were as follows:
1, Such basic statistical analysis as the mean, variance, standard deviation, CV(coefficient of variation)to the morphological characters of 16 variation types of Ph.violascens indicated that great diversity existed among different variation types, with ranging from 10.1%-41.4%. The height under branch has the highest variation,Diameter at breast height and The plant height was followed. The variation types of Ph.violascens were divided into 5 clusters by morphological characteristics, and which together clusters with similar morphological characteristics.
2, Based on the surveys of bamboo shoots growth of 16 variation types of Ph.violascens, the results show that Ph.violascens cv. Wanggan, Ph.violascens cv. Hongke emerge shoots earlier than Ph.violascens cv. Atrovaginis. Variation of the emerging shoots rates and grown bamboo rates are remarkable in different variation types.
3, Genetic diversity of 16 variation types of Ph.violascens was evaluated using AFLP, SRAP and ACGM markers. 15 AFLP primer pairs, 15 SRAP primer pairs and 38 ACGM primers generated 253, 152 and 75 polymorphic bands, respectively. These 3 molecular markers had consistent result from the clustering analysis by UPGMA, the significant correlation between AFLP, SRAP, and ACGM markers was observed. UPGMA method cluster analysis showed: (1) There are abundant genetic variation among different variation types of Ph.violascens; (2) The variation types of Ph.violascens were divided into 4 clusters by UPGMA.
引文
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