青海省主栽春油菜品种指纹图谱构建和纯度鉴定技术的研究
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摘要
我省低海拔地区主要种植甘蓝型油菜,品种多达7个,其中包括杂交种6个杂交种和常规种1个常规种。品种真伪及杂交种种子纯度鉴定,对于打击假冒伪劣,维护育种者的权益和保护农民的利益具有重要的意义。品种纯度的鉴定,传统的方法是采用田间种植鉴定,该方法可提供的检测指标有限,且准确性差。近年来发展的分子标记技术,能直接反映基因组水平的差异,已广泛应用到农作物品种指纹图谱构建和品种鉴定中,并成为杂交种真伪鉴定的有效手段。本研究应用SSR分子标记对我省目前广泛推广的甘蓝型油菜品种的指纹图谱及青杂系列杂交种种子纯度的快速鉴定技术进行了研究,得到以下结果:
(1)从140对SSR引物中筛选出13对多态性较好的引物用于指纹图谱的构建,发现同时采用P013、P052这2对引物组合或同时采用P086、P027和P021这3对引物组合,可以将14份供试材料(包括6个杂交种品种、7个亲本材料及1个常规种)完全区分开来。这些指纹可用于亲本材料和杂交种真伪的鉴别。
(2)筛选出在各品种中具有共显性标记的引物:青杂2号(P019、P027),青杂3号(P013、P027、P052),青杂4号(P027、P052、P086),青杂5号(P030、P085)。
(3)利用获得的共显性引物对2008年大田生产杂交种的纯度进行鉴定,同时也采用传统田间种植的方法对杂交种进行纯度鉴定。实验室检测各杂交种纯度分别为:青杂2号纯度:83.41%(P027)、83.90%(P019);青杂3号纯度:87.02%(P027)、86.54%(P052)、86.06%(P013);青杂4号纯度:70.67%(P027)、69.23%(P052)、68.75%(P086);青杂5号纯度:85.37%(P030)、82.93%(P085)。田间鉴定各杂交种纯度分别为:青杂2号88.29%、青杂3号91.35%、青杂4号72.12%、青杂5号87.80%。实验室分子标记检测结果与田间种植鉴定结果趋势基本一致。但实验室分子标记检测结果均较田间鉴定结果偏低,造成这种差异的原因主要是田间鉴定以育性为依据,即田间表现可育为真杂种、田间表现不育为假杂种,而分子标记检测不仅可以区分可育与不育单株,而且还可以将可育株中的真杂种和恢复系单株、保持系单株及杂单株区分开。
(4)将田间表现为可育而分子标记检测为父本带型(推测为恢复系)、田间表现为可育而分子标记检测为母本带型(推测为保持系)的单株分别与不育系测交,并自交,在云南鉴定测交F1和自交种的育性,结果表明推测为保持系单株的测交F1全为不育株,自交种全部可育;而推测为恢复系单株的测交F1全为可育株,自交种全部可育。证明了分子标记鉴定结果的准确性,同时也说明,相对于传统的田间种植鉴定方法,采用SSR分子标记进行杂交种纯度的鉴定更为准确。
In lower altitude areas of Qinghai Province where mainly planting Brassica napus, there are seven varieties that contains six hybrids, one conventional variety. identificate Hybrids authenticity and purity rapidly that it is important for fight against counterfeiting and protected the interests of breeders and farmers has grateful significance. In the past mainly testing purity use morphological in the field and that can offer limited detection targets, morphological identification way can not adapt for purity analysis more and more. Molecular markers have been developed in recent years, this way reflection the differences of genomic directly that has been applied to constructed agriculture crops and varieties fingerprinting and identification widely, that is an effective mean for hybrids authenticity identification. In this study, application of SSR molecular markers constructed 14 Cultivars spring Brassica napus fingerprints of Qinghai and study on Qingza lines hybrids rapid purity identification way, as the following results:
(1) 13 pairs polymorphic primers screened from 140 pairs SSR primers that using Construction fingerprints showed that the primer combinations P013 + P052 or P086 + P027 + P021 amplification bands can be completely distinguished14 materials that contains 4 hybrids and its parents, Huaxie No1 hybrids,Hufeng010 hybrids and Qingyou No14. Those fingerprints can be used for parent materials and hybrids authenticity identification.
(2) Selected SSR primers have co-dominant bands that adapt to varieties purities identification. Qingza No.2 hybrid (P019、P027), QingzaNo.3 hybrid (P013、P027、P052),Qingza No. 4 hybrid(P027、P052、P086) ,Qingza No.5 hybrid (P030、P085).
(3) Using above primers identified the hybrids purities that production of 2008, by field cultivation methods identified those hybrids Purity meanwhile. Laboratory testing hybrid purity: Qingza 2 Purity:83.41%(P027)、83.90%(P019);Qingza No. 3 purity:87.02%(P027)、86.54%(P052)、86.06%(P013);Qingza No. 4 purity:70.67%(P027)、69.23%(P052)、68.75%(P086);Qingza No. 5 purity:85.37%(P030)、82.93%(P085).Respectively, the field identification purities were 88.29%, 91.35%, 72.12%, 87.80%;through comparison lab. and field purities identification results, indicated that SSR markers tested purities all lower than field purities, that because of field planting identification purity according for saxes, and SSR marker not only distinct sterile from hybrids, but also districted more Restorer, and other rape plants in the field.
(4) 2009 winter in Yunnan tested materials that from Qingza No. 2 field tested hybrids, lab. tested sterile bands and restorer bands plants that crossed with true sterile, and tested self crossed seeds that from those plants self cross. Indicated that those hybrids from lab. tested sterile bands plants crossed with true sterile were all true sterile, self cross seed were all maintainers, and hybrids from lab. tested restorer bands plants crossed with true sterile were all true hybrids, self cross seed were all restorer. Indicated that SSR marker testing hybrid purity was correct than field plant testing.
(1)从140对SSR引物中筛选出13对多态性较好的引物用于指纹图谱的构建,发现同时采用P013、P052这2对引物组合或同时采用P086、P027和P021这3对引物组合,可以将14份供试材料(包括6个杂交种品种、7个亲本材料及1个常规种)完全区分开来。这些指纹可用于亲本材料和杂交种真伪的鉴别。
(2)筛选出在各品种中具有共显性标记的引物:青杂2号(P019、P027),青杂3号(P013、P027、P052),青杂4号(P027、P052、P086),青杂5号(P030、P085)。
(3)利用获得的共显性引物对2008年大田生产杂交种的纯度进行鉴定,同时也采用传统田间种植的方法对杂交种进行纯度鉴定。实验室检测各杂交种纯度分别为:青杂2号纯度:83.41%(P027)、83.90%(P019);青杂3号纯度:87.02%(P027)、86.54%(P052)、86.06%(P013);青杂4号纯度:70.67%(P027)、69.23%(P052)、68.75%(P086);青杂5号纯度:85.37%(P030)、82.93%(P085)。田间鉴定各杂交种纯度分别为:青杂2号88.29%、青杂3号91.35%、青杂4号72.12%、青杂5号87.80%。实验室分子标记检测结果与田间种植鉴定结果趋势基本一致。但实验室分子标记检测结果均较田间鉴定结果偏低,造成这种差异的原因主要是田间鉴定以育性为依据,即田间表现可育为真杂种、田间表现不育为假杂种,而分子标记检测不仅可以区分可育与不育单株,而且还可以将可育株中的真杂种和恢复系单株、保持系单株及杂单株区分开。
(4)将田间表现为可育而分子标记检测为父本带型(推测为恢复系)、田间表现为可育而分子标记检测为母本带型(推测为保持系)的单株分别与不育系测交,并自交,在云南鉴定测交F1和自交种的育性,结果表明推测为保持系单株的测交F1全为不育株,自交种全部可育;而推测为恢复系单株的测交F1全为可育株,自交种全部可育。证明了分子标记鉴定结果的准确性,同时也说明,相对于传统的田间种植鉴定方法,采用SSR分子标记进行杂交种纯度的鉴定更为准确。
In lower altitude areas of Qinghai Province where mainly planting Brassica napus, there are seven varieties that contains six hybrids, one conventional variety. identificate Hybrids authenticity and purity rapidly that it is important for fight against counterfeiting and protected the interests of breeders and farmers has grateful significance. In the past mainly testing purity use morphological in the field and that can offer limited detection targets, morphological identification way can not adapt for purity analysis more and more. Molecular markers have been developed in recent years, this way reflection the differences of genomic directly that has been applied to constructed agriculture crops and varieties fingerprinting and identification widely, that is an effective mean for hybrids authenticity identification. In this study, application of SSR molecular markers constructed 14 Cultivars spring Brassica napus fingerprints of Qinghai and study on Qingza lines hybrids rapid purity identification way, as the following results:
(1) 13 pairs polymorphic primers screened from 140 pairs SSR primers that using Construction fingerprints showed that the primer combinations P013 + P052 or P086 + P027 + P021 amplification bands can be completely distinguished14 materials that contains 4 hybrids and its parents, Huaxie No1 hybrids,Hufeng010 hybrids and Qingyou No14. Those fingerprints can be used for parent materials and hybrids authenticity identification.
(2) Selected SSR primers have co-dominant bands that adapt to varieties purities identification. Qingza No.2 hybrid (P019、P027), QingzaNo.3 hybrid (P013、P027、P052),Qingza No. 4 hybrid(P027、P052、P086) ,Qingza No.5 hybrid (P030、P085).
(3) Using above primers identified the hybrids purities that production of 2008, by field cultivation methods identified those hybrids Purity meanwhile. Laboratory testing hybrid purity: Qingza 2 Purity:83.41%(P027)、83.90%(P019);Qingza No. 3 purity:87.02%(P027)、86.54%(P052)、86.06%(P013);Qingza No. 4 purity:70.67%(P027)、69.23%(P052)、68.75%(P086);Qingza No. 5 purity:85.37%(P030)、82.93%(P085).Respectively, the field identification purities were 88.29%, 91.35%, 72.12%, 87.80%;through comparison lab. and field purities identification results, indicated that SSR markers tested purities all lower than field purities, that because of field planting identification purity according for saxes, and SSR marker not only distinct sterile from hybrids, but also districted more Restorer, and other rape plants in the field.
(4) 2009 winter in Yunnan tested materials that from Qingza No. 2 field tested hybrids, lab. tested sterile bands and restorer bands plants that crossed with true sterile, and tested self crossed seeds that from those plants self cross. Indicated that those hybrids from lab. tested sterile bands plants crossed with true sterile were all true sterile, self cross seed were all maintainers, and hybrids from lab. tested restorer bands plants crossed with true sterile were all true hybrids, self cross seed were all restorer. Indicated that SSR marker testing hybrid purity was correct than field plant testing.
引文
[1]王汉中.入世后的中国油菜产业[J].中国油料作物学报,2002,24(2)
[2]傅廷栋.杂交油菜的育种与利用[M].武汉:湖北科学技术出版社,1994,1～3,173～175
[3]杨光圣,傅廷栋.环境条件对油菜细胞质雄性不育的影响[J].中国油料,1987,(3):15～19
[4]雷天刚.甘蓝型黄籽油菜SSR和同工酶标记的遗传多样性和指纹图谱:[硕士学位论文].重庆:西南农业大学,2005
[5]陈卫江,李莓,易冬莲.纯度对甘蓝型油菜质不育三系杂种产量的影响[J].湖南农业科学,2002,(3):6～7,10
[6]李冬肖,王钊,刘玉风.F1不育株对油菜杂交种产量影响程度的分析与预测[J].西北植物学报,1996,16(6):132～135
[7] Tanksley S D., McCouch S R. Seed banks and molecular maps:unlocking genetic potential from the wild.Science,1997,277(5329):1063～1066
[8]刘勋甲等,郑用琏,尹艳.遗传标记的发展及分子标记在农作物遗传育种中的运用Ⅰ遗传标记的发展及分子标记的检测技术(续)[J].湖北农业科学,1998,(1):33-35,(3):27-32
[9]梁明山,刘煜,候留记,等.2001聚丙烯酰胺凝胶电泳鉴定烟草品种[J].种子, 2001, (1)
[10]傅廷栋.杂交油菜的育种与利用[M].武汉:湖北科学技术出版社,1994,89～90
[11]于滟,曾凡亚,赵云,等.杂交油菜“蜀杂6号”特异序列扩增标记(SCAR)建立及其在杂种鉴定中的应用.作物学报,2001, 27(6):722～728
[12]黄三文,张宝玺,郭家珍,等.辣椒RAPD系统的建立及去杂种子纯度鉴定的应用.园艺学报,2001,28(1):77～79
[13]刘杰,刘公社,李殿荣,等.RAPD方法鉴定油菜“杂油59”种子纯度的研究[J].应用与环境生物学报,2000,6(6):526-529
[14]段院生,刘平武,杨光圣.甘蓝型油菜杂交种亲本指纹图谱分析[J].中国油料作物学报,2002, 24(2):5～9
[15]Willams J.G.W, kubelic A.R,.DNA polymorphisms amplified by arbitrary are useful as genetic marker. Nucleic Acids Res,1990,(18):6531～6535
[16]Vos P, Hogers R,B, leeker M,, et al. AFLP a new technique for DNA fingerprinting. Nucleic Acids Res,1995,(23):4407～4414
[17] Paran I, Michelmore R W. Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theoretical and Applied Genetics,1993,( 85):985-993
[18]文雁成,王汉中,沈金雄,等.SRAP和SSR标记构建的甘蓝型油菜品种指纹图谱比较[J].中国油料作物学报,2006,28(3):233～239
[19]雷天刚,张学昆,陆合.甘蓝型油菜黄籽品种SSR指纹图谱的构建[J].西南农业大学学报(自然科学版),2005, 27 (3) : 361～364
[20]谭君,丁仲芳,孙仕贤,等.西南常用玉米自交系SSR指纹图谱构建[J].西南农业学报,2003,16(2): 1～6
[21]关荣霞,刘燕,刘章雄,等.利用方法鉴定大豆品种纯度[J].分子植物育种,2003,3(1):357～360
[22]许占友,李磊,常汝镇,等.大豆质核互作雄性不育系核不育基因的遗传分析[J] .中国农业科学,1999 ,32(增刊) :1～8
[23]苏顺宗,黄玉碧,杨俊品,等.利用SSR鉴定水稻杂交种子纯度的研究[J].种子, 2003, (1): 23～25
[24]梅德圣,李云昌,胡琼,等.甘蓝型油菜中油杂8号种子纯度的SSR鉴定[J].中国农学通报,2006,22,49～52
[25]Vandberg. J.L. Biochemical markers and restriction fragment length polymorphisms in baboons : Paternity in Primates: Genetic Test and Theories-implications of Human DNA Fingerprinting 1992.
[26]陈家宽,杨继主编.植物进化生物学[M].武汉大学出版社, 1994,153～194
[27]王中仁编,植物等位酶分析[M].北京:科学出版社, 1996,69～73
[28]Gooke R J, Gel electrophoresis for the identification of plant variety. Journal of chromatography A, 1995,698:281～229
[29]胡国之.金针茹品系间的酯酶同工酶标记筛选研究[J].生物学杂志,1999,16(1):13～14
[30]谢宗铭.生化指纹在向日葵育种上的应用L同工酶的研究和应用.作物杂志, 1999,69(2):1～4
[31]Gooke R J. Handbook of variety testing[J].ISTA,Swizterland,1992
[32] Bury W J,Bino R J.ISTA/LAHS Symposium: Technological Advances in Variety and Seed Testing. Wageningen, The Netherlands,1994,36～70
[33]于新艳,王凤格,赵久然等.SSR标记及其在玉米研究中的应用[J].安徽农业科学,2007,35(7):3
[34]宇文强.贵州杂交玉米纯度检验及其指纹图谱的构建:[硕士学位论文].云南:贵州大学,2006
[35]孔祥彬.利用SSR指纹技术构建玉米品种指纹图谱及遗传多样性研究:[硕士学位论文].山东:山东农业大学,2006
[36]李根英, Susanne Dreisigacker,Marilyn,等.小麦指纹图谱数据库的建立及SSR分子标记试剂盒的研发[J] .作物学报, 2006, 132(112): 1771-177
[37]李伟,郑有良,魏育明,等.小麦新品种川农16的分子鉴定[J].麦类作物学报, 2004,24(1):6-10
[38]李莉,杨剑波,Machill D.J.,Colow it P.M..水稻SSR不同检测和分析方法的比较研究[J].中国水稻科学, 2000,14(3):185-188
[39]PlieskeJ.,Struss D.,2001,Microsatellite markers for genome analysis in Brassica.I.developmentin Brassica napus and abundance in Brassicaceae species,Theor.App1.Genet.,102(4):689-694
[40]王风格,赵久然,郭景伦,等.中国玉米新品种标准DNA指纹库构建研究的几点思考[J].植物学通报,2005, 22(1): 121-128
[41]Provan J., Powell W. ,and Waugh R.,1996,Microsatellite analysis of relationship swith in cultivated potato (Solanumtuberosum), Theor.App1.Genet.,92(8):1078-1084
[42]王俊芳.棉花品种指纹图谱构建及棉种鉴定技术研究[J].2009, 36(3)∶6~24
[43]崔艳华,邱丽娟,常汝镇,等.利用SSR分子标记检测黄淮夏大豆初选核心样本的代表性[J].植物遗传资源学报, 2003,4(1):9-15
[44]王玺,张宝石.玉米种子纯度鉴定技术研究进展[J].种子,2002,1:43～44
[45]富昊伟.利用芽鞘及不完全叶颜色的差别鉴定水稻特定杂交组合种子纯度[J].杂交水稻,2002,17(4):27
[46]李名胜,朱宗同.几个西瓜群体材料的叶形变异与杂种纯度鉴定[J].安徽农业科学,2001,29(2),213～216
[47]马志虎,颜素芳,沈晓昆,等.辣椒黄绿苗突变体叶色标记技术在种子生产中的作用[J].种子世界,2001,9:19～20
[48]崔洪昌、郑晓鹰.大白菜种子蛋白分离的高效液相色谱法的研究[J].种子,1993,(4):7～11
[49]崔洪昌,郑晓鹰.高效液相色谱法在大白菜种子品种鉴定中的应用[J].种子,1993,5:1～3
[50]文方德,李卓杰,傅家瑞.种子纯度鉴定技术进展及其评论[J].种子,1995,5:36-38
[51]颜启传.作物品种纯度检验的进展[J].种子,1984(2):61
[52]颜启传,黄亚军.农作物品种鉴定手册[M].北京:中国农业出版社,1996
[53] O.ulvin,Handbook of seed Testing一Testing for Genuineness of Cultivar[J]. ISTA Secretary,1973
[54] Laser K.D.,Lersten N R. Anatomy and cytology of microspore genesis in cytoplasmic male sterile angiosperms [J]. Bot Rev,1972,38:427～454
[55]余凤群,傅廷栋.甘蓝型油菜几个雄性不育系花药发育的细胞形态学研究[J].武汉植物学研究,1990,3(3):209～215
[56]危文亮,王汉中,刘贵华.甘蓝型油菜细胞质雄性不育系NCa花药发育的细胞学观察[J].中国农业科学,2005,38(6):1232～1237
[57]杨光圣,瞿波,傅廷栋.三个甘蓝型油菜隐性细胞核雄性不育系小孢子发生的细胞学研究[J].华中农业大学学报,1999,18(6):520～523
[58]杨光圣,瞿波,王淑新,等.一个甘蓝型油菜DGCMS雄性不育系的花器形态及细胞学研究[J].中国油料作物学报,2000,22(3):16～21
[59]袁本威.甘蓝型油菜胞质雄性不育的细胞学观察[J].陕西农业科学,1991,(2):8～9
[60]黄邦全.甘蓝型油菜雄性不育材料S90-8-7的获得及细胞学研究[J].湖北大学学报(自然科学版),2000,22(2):182～184
[61]官春云,李栒，王国槐等.化学杂交剂诱导油菜雄性不育机理的研究.I.杀雄剂1号对甘蓝型油菜花药绒毡层和花粉粒形成的影响[J].作物学报,1997,(5):513～521
[62]李红兵.陕油8号种子纯度鉴定:[硕士学位论文].陕西:西北农林科技大学, 2005
[63]李淑娟.种子纯度鉴定方法概述[J].青海大学学报,2003,21(l)16～19
[64]Cooke J.杜充译.鉴定农作物栽培品种的电泳方法[J].国外遗传与育种.1985,1:14-20
[65]郭艳.陕油10号种子纯度鉴定: [硕士学位论文].陕西:西北农林科技大学, 2007
[66]汤陵华.亚洲主要稻作国家栽培稻在同工酶顶点的差异[J].作物学报,1991,17(6):9-16
[67]邵继荣,何清海,张述华,等.水稻多用恢复系130的七种同工酶研究[J].西南农业学报,1999,12(1):8-13
[68]岳平,宋希云,张爱民,等.用SDS-PAGE方法进行杂交小麦纯度鉴定[J].北京农业大学学报,1993,(9):59.
[69]王景升,王玺,刘国奇,等.电泳谱带法在作物品种纯度鉴定上的应用研究[J].沈阳农业大学学报,1996,(3):221-225.
[70]周展明.玉米蛋白质凝胶电泳法鉴定品种纯度的研究[J].种子世界,1992,(1):16-18.
[71]李殿荣,夏永真,王保仁.利用酯酶同工酶谱分析鉴定杂交油菜秦油二号种子纯度的方法[J].陕西农垦科技,1994,(24):10～12.
[72]梅德圣,李云昌,李应德,等.用酯酶同工酶和AFLP标记鉴定甘蓝型油菜“中油杂2号”的种子纯度[J].江西农业大学学报,(27): 1.
[73]漆小泉,徐光,郭家珍等.用α-淀粉酶同工酶分析测定中椒6号种子的品种纯度[J].中国蔬菜,1994,(2):9～10.
[74]马德伟,黄锁柱,孙岚,等.辣椒遗传性的种子醇溶蛋白电泳研究[J].园艺学报,1995,2(23):297～298.
[75]胡辉林,党润盈.方法鉴定杂交油葵及其2亲本品种纯度的研究[J].中国油料作物学报,2002, 24(2):26～28.
[76]段维,谢宗铭,陈福隆,等.利用种子蛋白丙烯酰胺凝胶电泳鉴定向日葵种子纯度的研究[J].种子,2001,(1):24～25.
[77]Anisimova,I.N, Gavrilyuck IP, Konarev CG. Identification of sunflower lines and varieties by helianthemion electrophoresis[J].PlantVarieties&Seeds,1991,(4):133～141.
[78]Varier A, Dadlani M, Cooke RJ. Identification of sunflower hybrids and inbreeds by SDS-PAGE of the seed proteins[J].Seed Research,1992,20(2):138～141.
[79]赵建宗,付友兰.水溶蛋白电泳鉴定杂交油葵种子纯度[J].种子科技,2003,21(5):284～285.
[80]纪颖彪,朱其杰.同工酶分析在黄瓜杂种一代纯度检测上的应用研究[J].园艺学报,1995,22(3):250～255.
[81]郑晓鹰,李丽,李秀清.大白菜品种同工酶及水溶蛋白的遗传多样性[J].园艺学报,1997,24(3):244～248.
[82]文方德,李卓杰,傅家瑞.用ACP及ADH同工酶等电聚焦快速鉴定番茄杂种纯度[J].园艺学报,1996,23(2):202～204.
[83]Tanksley S.D. , Jones R.A. .Application of ADH allozymes in testing the genetic purity of F1 hybrids of tomato. Hort [J].Science,1981,16(2):179～181
[84]李丽,郑晓鹰,邢宝田.蛋白电泳技术在F1代杂交种纯度鉴定中的应用[J].种子检验,2000,18:51-53
[85]彦启传.种子检验的原理和技术[M],北京:农业出版社,1992
[86]陆士伟,黄炳权,邝章标.应用酯酶同工酶测定杂交水稻杂种纯度的研究[J].中国农业科学,1982,5:10～16
[87]李卓杰,傅家瑞等.电聚焦电泳对杂交水稻种子纯度鉴定的技术[J].种子,1991,(3):68
[88]玉米种子盐溶蛋白电泳鉴定方法,NY/T449-2001[S].农业部,2001.
[89]Anisimova,I.N, Roger,J., Fido., et al. Variation and polymorphism of 2S Albumins of sunflower [J].Euphytica,1995,( 83):15～23.
[90]李殿荣,夏永真,王保红.利用酸酶同工酶谱分析鉴定杂交油菜秦油8号种子纯度的方法[J].陕西农垦科技,1994,24(杂交油菜专辑):10～12
[91]穆建新.利用分子标记技术分析鉴定杂交油菜种子纯度研究:[硕士学位论文].陕西:西北农林科技大学, 2005
[92]贾继增.分子标记种质资源鉴定和分子标记育种[J].中国农业科学, 1996,29(4):1～10
[93]方宣钧,吴为人,唐纪良.作物DNA标记辅助育种[M].北京:科学出版社, 2001.
[94]胡胜武,赵惠贤,路明.分子标记在芸荃属植物遗传育种中的应用[J].中国油料作物学报,2001,23(4)82～86
[95]刘春吉,王明利.RFLP分析过程中应注意的几个问题[J].遗传,1994,16(6):37～40.
[96]张修军.微生物融合重组子的鉴定—DNA分子检测技术[1J.生物学杂志,1999,16(2):5～7.
[97]刘旭.遗传标记和遗传图谱构建[J].作物品种资源,1997,(3):29～32.
[98]吴平.应用RFLP标记分析水稻株高与分蘖的遗传相关性[J].中国科学(C辑), 1996,26(3):257-263
[99]宣朴,瞿世洪.植物育种中的RFLP技术[J].西南农业学报,1994,7(2):106～112
[100]Sasaki T, Abstact of plant GenomeⅢ[M], San Diego USA, 1995,11
[101]Song Y C, The physical location of 14 RFLP markers in rice, Theory Appl Genet, 1995,90(1):113～119
[102]Gale M D, Genetic maps of hexaploid wheat,proc.8th,interm,wheat Genet samp, beijing, 1993,29～31
[103]Peterson L.Genetic diversity among wild and cultivated barley as revealed by RFLP. Theory Appl Genet,1989,(6):679～681
[104]Graner A, Jaboor A, schondelmaier J. construction of an RFLP map of barley, Theory Appl Genet, ,1991,83(2):250～256
[105]Keim P, Bearis W D, Schupp J M. RFLP analysis of soybean breeding populations: I. Genetic structure differences due to inbreeding methods. Crop Sci,1994,( 34):55～61.
[106]Mansur L M. Determining the linkage of qualitative trait loci to RFLP markers using extreme phenotypes of recombinant inbreds of soybean. Theory Appl Genet, 1993,86(8):914～918.
[107]Leon A J. Use of RFLP markers for genetic linkage analysis foil percentage in sunflower seed. Group Sci,1995,35(2):558～564
[108]sarfatti M, katan J, Fluhr R. A RFLP markers in tomato linked to the fusarium axysporum resistance gene 12,Theory Appl Genet, 1989,78(5):755～759
[109]Gebardt C., Blomendahl C., Schachtschabeo U., et.al. Identification of 2n breeding lines and 4n varieties of potato (Sloane tuberosum, ssp. tuberosum) with RFLP fingerprinting[J].Theory ApplGenet,1989,( 78):16～22
[110]田志宏,郑用琏,孟金陵.几个甘蓝型油菜细胞质雄性不育系线粒体DNA的RFLP分析[J].遗传,1998,20(增刊):22～26
[111]Welsh J., Mcclelland M., Finger Printing genomes using PCR with arbitrary primers. Nuel Acids Des.1990,18(24):7213～7218
[112]Sun G L, Fahima T, kord A B. Identification of molecular markers linksed to the Yr stripe rest resistance gene of wheat originated in wild emmer wheat, Triticum dicoccoises, Theory Appl Genet, 1997,(95):622～628;
[113]Vierling R A, Nguyen. Use of RAPD markers to determine the genetic diversity of diploid, wheat genotypes, Theory Appl Genet,1993,84(7-8):835～838
[114]张秀英,葛春民,贾继增.山羊草属五个基本基因组RAPD分析[J],植物学报,1999,41(4),393～397
[115]夏光敏,向凤宁,周爱芬.1999,小麦与高兵草属间体细胞胞杂交获可育杂种植株[J],植物学报,41(4),349～352
[116]Welsh.J.,Honeycutt R J,Mcdelland M. Percentage deternimation in maize hybrids using the arbitrarily primed polymerase chain reaction. Theory Appl Genet.82(4):573～576
[117]Vierling R A. Genetic diversity among elite sorghum lines revealed by restriction fragment length polymorphisms and random amplified polymorphic DNAs. Theory Appl Genet, 1994,87(7):816～820
[118]Yu L X, Nguyen. Genetic variation detected with RAPD markers among uplang ang lowland rice cultivars. Theory Appl Genet, 1992 ,87(6):668～672
[119]朱诚,刘之燕.RAPD技术与桂花品种分类和鉴定[J].广西植物, 1999,19(2):190～192
[120]Marshall P, Marchand M C, Lbieczko Z. A simple method to estimate the percentage ofhybridity in canola(Brassica napus)F1 hybrids. Theory Appl enet,1994,(98):853～858
[121]李小彬,周小梅等.水稻三系及其杂种的RAPD指纹图谱分析[J].农业生物技术学报,1995,3(2):1～6
[122]杨蜀岚,伏健民,杨仁崔,等.矮64S为不育系的两系杂交稻纯度的RAPD鉴定[J].福建农业大学学报,1998,27(1):6～9
[123]陈忠明,王秀娥,赵彦,等.两优培九种子纯度的RAPD快速鉴定.杂交水稻,2003,18(4):55～56
[124]钱前,陈洪,孙家修,等.真假杂交水稻n优63的ARPD鉴定[J].中国水稻科学.1996,10(4):241～242.
[125]李文杉,周小梅.水稻三系及其杂种的ARPD指纹图谱分析[J].农业生物技术学会,1995,3(2):l～6
[126]扬剑波,李莉等.利用ARPD技术检测杂交水稻种子纯度[J].安徽农业科学,1996,24(3):193～195.
[127]徐鑫,刘学群,瞿波,等.ARPD标记鉴定水稻3种不同细胞质雄性不育类型的杂交种及其亲本[J].遗传,2005,27(3):377～381.
[128]吴敏生,戴景瑞,王守才.RAPD在玉米品种鉴定和纯度分析中的应用[J].作物学报,1999,25(4):89～493,
[129]王凤格,赵久然,郭景伦,等.中国玉米新品种DNA指纹库建立系列研究[J],玉米科学,2003,11(2):3～6
[130]Marshall P,Marchand M C,Lbieczko Z.A simple method to estimate the percentage of hybridity in canola(Brassica napus)F1 hybrids.Theor Appl enet,1994,98:853～858,
[131]Mail R.J, Scarth R. Discrimination among cultivars of rapeseed(bressica.napus)using DNA polymorphism amplified from arbitrary primers. Theory Appl Genet,1994,( 87):694～704
[132]赵洪锟,李启云,王玉民,等.RAPD标记在大豆品种鉴定中的应用初探[J].吉林农业科学,1998(4):6～8
[133]易成新,张元真.分子标记用于棉花杂交种种子纯度测验的初步研究[J].棉花学报,1999,11(6):318～320
[134]庄木,王晓武,杨丽梅,等.利用RAPD方法鉴定两个甘蓝品种的纯度[J].中国蔬菜,1999(5):8～9
[135]范丙友,刘玉梅,高水平.RAPD技术在我国主要蔬菜遗传育种研究中的应用[J].中国蔬菜,2002(3):55～56
[136]郑晓鹰,Klocke E..利用RAPD分子标记对番茄杂交种纯度的鉴定研究[J],广西植物,2003,23(2):149～154
[137]刘富中,刘万勃,宋明,等.利用RAPD快速鉴定甜瓜杂交种的遗传纯度[J],中国蔬菜,2002,(5):7～9
[138]柳李旺,龚义勤,雷春,等.辣椒F1杂种遗传纯度的种子蛋白、同工酶与RAPD鉴定[J].分子植物育种,2003,1(5/6):663～667
[139]欧阳新星,许勇,张海英,等.应用RAPD技术快速进行西瓜杂交种纯度鉴定研究[J],农业生物技术学报,1999,7(1):23～28
[140]周群初,何清,马艳青,等.利用RAPD技术辣椒杂种纯度鉴定的研究[J].湖南农业科学,1999(5):14～15
[141]蒲汉丽,李乃坚,黄爱兴,等.RAPD技术鉴定番茄一代杂种奥星种子纯度的研究[J].种子,2000(5):14～16
[142]李成伟,李卓杰.用RAPD方法鉴定杂交辣椒种子纯度[J].种子, 1999(2): 5-6
[143]李传勇,黄聪丽,潘爱民,等.利用RAPD分析鉴定花椰菜杂种纯度[J].亚热带植物科学,2001,3(04):1～4
[144]孙敏,齐爱民,王和勇,等.黄瓜杂交种子纯度的RAPD鉴定[J].西南师范大学学报,2003,28(1)
[145]Crockett P.A, Bhalla P.L., Lee C.K., ea tl. RAPD analysis of seed purity in a commercial hybrid cabbage(Brassica oleracea var.captitata)cultivar[J].Genome,2000,( 43):317～321
[146]Hasgizume T., Sato T., Hirai M. Determination of genetic purity of hybrid seed in watermelon(Citrulluslanatus)andTomato(Lycopersiconesculentum)usingRAPD.Japan.J.Breed.1993,( 43):367～375
[147]Philllip A. Crockett,Prem L. Bhalla,O.K. RAPD analysis of seed purity in a commercial hybrid cabbage(Brasslca oleracea var. capitata)cultivar,Genome.2000,43(2):317～321
[148]邵启明,裴炎,侯磊,等.汕优63的DNA分子标记及种子纯度鉴定研究[J].西南农业大学学报,1996,18,(2)
[149]莫结胜,刘杰,刘公社.杂交油葵A15种子纯度的RAPD鉴定[J].作物学报,2001, 27,( 1):85～90
[150]Hu J., Quiros C.F. Identification of broccoli and cauliflower cultivars with RAPD markers, Plantcell Report,1991,(10):505～511
[151]Choe Y W, Park D Y, Suh K H. Pepper(Capsicum annuum L)cultivar identifacation and seed purity test by random amplified polymorphic DNAs (RAPDs)and phosphoglucomutase(PGM) isozyme, RDA Journal of Horticulture Science, 1998,40(1):15～23
[152]Meng X.D, Ma H, Zhang W.H. A fast procedure for genetic purity determination of head Chinese cabbage hybrid seed based on RAPD markers, Seed Sci.&Technol.,1998,26:829～833
[153]栾雨时,苏乔,李海涛,等.利用RAPD技术快速鉴定番茄杂种纯度[J],园艺学报,1998,25(3):247～250
[154]邓义才,王得元,李乃坚,等.利用RAPD技术鉴定早青三号黄瓜种子纯度的研究[J].广东农业科学,1999,(3):17～18
[155]李红兵,董振生,陈毓荃,等.陕油8号种子纯度的ARPD鉴定研究[J].西北植物学报,2004,24(11):2007～2011.
[156]张修军.微生物融合重组子的鉴定—DNA分子检测技术.生物学杂志, 1999,16(2):5～7
[157]王苏建,随机扩增DNA多态性及其在微生物分型中的作用.国外医学(微生物分册),1999,22(2):18～20
[158]张恒庆,红松RAPD实验中各组成份含量对实验结果的影响,植物研究,1999,19(2):183～188
[159]Tautz D. Hyper variability of simple sequences as a general source for polymorphic DNA markers[J]Nucleic Acids Research,1989,(17):6463-6471.
[160]Litt M,Luty J A.A hypervariable microsatellite revealed by invitro amplification of dinucleotide repeat within the cardiac muscle actln gene[J].American Journal of HumanGenetics,1989,(44):397-401 .
[161]李汝玉,杨平平,宋国安,等.简单序列重复(SSR)及其在品种鉴定及种子检测中的应用[J].种子,1999(5):33-35.
[162]Tautz D, Renz M. Simple sequences are ubiquitous repetitive components of eukaryotic genomes[J]Nucleic Acids Research,1984, (12):4127-4138.
[163]张立荣,徐大庆,刘大群. SSR和ISSR分子标记及其在植物遗传育种研究中的应用[J].河北农业大学学报,2002,25(1):90-94
[164]万平,刘大钧等.SSR标记与植物遗传育种研究[J].安徽农业大学学报,1998,25(1):92-95
[165]Gregan J. The Abstract of Molecular Biology symposium. Amsterdam, The Netherland, 1994,19～46
[166]辛景树,郭景伦,张软斌.几种常用分子标记技术在种子纯度和品种真实性鉴定方面的比较与分析[J].种子,2005.24(1):58-60
[167]BellCJ,EekenJR. Assignment of 30 micresatellite loci to the linkage map of Arabidopsis [J].Genomics,1994,19:137～144
[168]Wangz,WeberJL,ZhongG,etal. Survey of Plant short natdem DNA erpeat [J].Theory APPL Genet,1994,(88):1～6
[169]李文涛,张桂权.水稻微卫星标记的发展和应用[J].生命科学,2000,12(5) :234～237
[170]赵勇,杨凯,翁跃进.利用水稻功能基因SSR标记鉴定水稻种质资源[J].中国农业科学,2002,35(4):349～353
[171]李永强,李宏伟,高丽锋,等.基于表达序列标签的微卫星标记[J].植物遗传资源学报,2004,5(l):91～95
[172]番兴明,张世煌,谭静,等.根据SSR标记划分优质蛋白玉米自交系的杂种优势群[J].作物学报,2003,29(l):105～110
[173]刘世建,荣廷昭,杨俊品,等.四川地方玉米种质的SSR聚类分析[J].作物学报,2004,30(3):221～22
[174]高闻伟,李晓辉,田清震.利用SSR标记快速鉴定玉米杂交种农大108和豫玉27的种子纯度[J].种子,2004,23(5):6
[175]刘峰,陈受宜.大豆基因组中的微卫星标记[J].大豆科学,1998,17(3):256～261
[176]许占友,邱丽娟,常汝镇,等.利用SSR标记鉴定大豆种质[J].中国农业科学,1999,32(增刊:)40-48.
[177]关荣霞,刘燕,刘章雄,等.利用SSR方法鉴定大豆品种纯度[J].分子植物育种,2003,(1)3:357～360
[178]武耀廷,张天真,郭旺珍,等.陆地棉品种SSR标记的多态性及用于杂交种纯度检测的研究]JI.棉花学报,2001,13(3):13～133
[179]刘列钊,林呐.油菜简单重复序列ssR(simplesequenceerpeat)研究进展[J].生命科学,2004,16(3):173～176
[180]詹庆才.利用微卫星DNA标记进行杂交水稻种子纯度鉴定的研究[J].杂交水稻,2002,17(5):46～50
[181]李稳香,詹庆才.杂交水稻种子纯度SSR指纹图谱标记鉴定技术研究[J].中国种业,2006(3):30～31
[182]王凤格,赵久然.中国玉米新品种DNA指纹库建立系列研究Ⅲ.多重PCR技术在玉米SSR引物扩增中的应用[J].玉米科学,2003,11(4):3-6
[183]谭振馨,李汝玉,李群,等.玉米杂交种SSR标记纯度鉴定方法研究[J].山东农业科学,2004,(4):8～11
[184]高文伟,李晓辉,田清震,等.利用SSR标记快速鉴定玉米杂交种农大108和豫玉27的种子纯度[J].种子,2004(5):32～33
[185]秦利,李冰.新疆陆地棉SSR标记指纹图谱构建和杂种纯度鉴定研究[J].新疆农业科学,2005,42(6):399～401
[186]石海波,王立新等.利用SSR标记区别小麦品种种子混杂和SSR位点不纯的研究[J].分子植物育种,2006,(4):513～519
[187]艾呈详,陆璐.SSR标记技术在甜瓜杂交种纯度检测中的应用[J].园艺学报,2005,32(5):902～904
[188]刘平武,周国岭,杨光圣,等.双低甘蓝型油菜杂交种亲本指纹图谱构建和杂交种纯度鉴定[J].作物学报, 2005, 31 (5): 640-646
[189]Vos P.,Hogers R.,Zabeau M.,et al. AFLP:a new technique for DNA fingerprinting[J].Nucl Acids Res,1995,23(21):4407～4414
[190]MackillDJ. Level of Polymorphism and genetic mapping of AFLP mark esrinriee [J].Genome,1996,39(5):969～977.
[191]ChoYQBlarMW,PnaaudO·Cloning and mapping of variety一specific genomic DNA sequneees: Amplified refagmeni lenhgt polymoPhrism(SFLP)rfmosilver一stained Polyacyrlmaide gels[J].Genome,1996,(39):373～387
[192]MalleswaranM,SubudhiK,NnadiS. Polymoprhism,distribution and segregation of AFLP markers in adouble haploid rice PoPulation [J].Theory APPI Genet 1997,94(l):39～45
[193]Keim , PBearisWD , SchuPPJM.RFLP analysis of soybeen breeding populations:1.Genetic:1structure differences due to inbreeding methods[J].CorPSei,1994,(34):55～61
[194]Qix. Development of AFLP marker in barely[J].Molecular & General Genetics,1997,254(3):330～336
[195]Bachem C W B, Vander Hoeven RS, de Bruijin S M. Visualization of differentinal gene expression using a novel method of DNA fingerprinting based on AFLP, Analysis of gene expression during potato tuber development, Plant Journal, 1996,9(5),745～753
[196]尹佟明,黄敏仁. AFLP分子标记及其在植物育种上的应用[J] .生物工程进展,1997 ,17 :11～16
[197]张增翠和侯喜林.不结球白菜矮抗6号的AFLP指纹鉴定[J].江苏农业科学,2004,(1):63～64
[198]朱云国,王学德.用AFLP技术构建棉花雄性不育三系及其杂种Fl的DNA指纹图谱[J].棉花学报,2001,13(3):158～160
[199]刘杰,刘公社,齐冬梅,等.向日葵品种鉴定和纯度分析AFLP研究[]J.植物学通报,2002,19(4):444～451
[200]张峰.AFLP-银染法检测植物基因组多态性[J].细胞生物学杂志, 1999 ,21(2):98～100
[201]潘俊松,王刚,李效尊,等.黄瓜SRAP遗传连锁图的构建及始花节位的基因定位[J].自然科学进展,2005,15(2):167～172
[202]林忠旭,张献龙,聂以春。新型标记SRAP在棉花F2分离群体及吟唱多样性评价中的适用性分析[J].遗传学报,2004,31(6):622～626
[203]任羽,王得元,张银东,等.辣椒SRAP-PCR反应体系的建立与优化[J].分子植物育种,2004,2(5):689～693
[204]李严.张春庆.新型分子标记-SRAP技术体系优化及应用前景分析[J].中国农学通报,2005,5
[205]刘华,王宇生,张辉,等.中国小麦种质资源醇溶朊蛋白指纹图谱数据库的初步构建和应用[J].作物学报, 1999, 25(6): 674～682
[206]Mackill D.J..Level of polymorphism and genetic mapping of AFLP marker in rice[J].Genome,1996,39(5):969～977
[207]Zhu Z F, Sun C Q, Li Z C et al. Study on the classification of rice varieties with SSR molecular markers [J].Agric Biotech,2001,9(1):58～61
[208]王灏等,利用RAPD技术进行杂交油菜杂油59品种鉴定和纯度分析[J]中国农业科学,2002,12
[209]林道哲等,26份甘蓝型油菜自交系指纹图谱分析[J].安徽农业科学,2008,36(20)8493-8496
[210]王通强等,油菜杂种及亲本指纹图谱构建和杂种纯度鉴定[J].食品与生物技术学报, 2009,28(3)377-384
[211]Saghai-Maroof M A, Soliman K M, Jorgenson R A, Allard R W. Ribosomal DNA spacer-length polymorphisms in barley. Mendelian inheritance, chromosomal location, and populaion dynamics[J]. Proc Natl Acad Sci, 1984, 81: 8014-8018.
[212]Tautz D. Hypervariability of simple sequence as general souce for polymorphic DNA markers[J]. Nucleic Acids Res, 1989, 12: 6463-6467.
[213] BRANT JB,GUSTAVOCA,PETERMG. Fast and sensitive silver Staining of DNA in polyacrylamide gels[J].Anal Biochem.1991,19(6):80-83
[214]李进波.分子标记在水稻抗性育种及杂交稻种子纯度和真伪鉴定中的应用: [硕士毕业论文].武汉:中国农业科学院,2005
[215]苏顺宗.利用SSR分子标记鉴定杂交稻种子纯度的研究:[硕士毕业论文].四川:四川大学,2003
[2]傅廷栋.杂交油菜的育种与利用[M].武汉:湖北科学技术出版社,1994,1～3,173～175
[3]杨光圣,傅廷栋.环境条件对油菜细胞质雄性不育的影响[J].中国油料,1987,(3):15～19
[4]雷天刚.甘蓝型黄籽油菜SSR和同工酶标记的遗传多样性和指纹图谱:[硕士学位论文].重庆:西南农业大学,2005
[5]陈卫江,李莓,易冬莲.纯度对甘蓝型油菜质不育三系杂种产量的影响[J].湖南农业科学,2002,(3):6～7,10
[6]李冬肖,王钊,刘玉风.F1不育株对油菜杂交种产量影响程度的分析与预测[J].西北植物学报,1996,16(6):132～135
[7] Tanksley S D., McCouch S R. Seed banks and molecular maps:unlocking genetic potential from the wild.Science,1997,277(5329):1063～1066
[8]刘勋甲等,郑用琏,尹艳.遗传标记的发展及分子标记在农作物遗传育种中的运用Ⅰ遗传标记的发展及分子标记的检测技术(续)[J].湖北农业科学,1998,(1):33-35,(3):27-32
[9]梁明山,刘煜,候留记,等.2001聚丙烯酰胺凝胶电泳鉴定烟草品种[J].种子, 2001, (1)
[10]傅廷栋.杂交油菜的育种与利用[M].武汉:湖北科学技术出版社,1994,89～90
[11]于滟,曾凡亚,赵云,等.杂交油菜“蜀杂6号”特异序列扩增标记(SCAR)建立及其在杂种鉴定中的应用.作物学报,2001, 27(6):722～728
[12]黄三文,张宝玺,郭家珍,等.辣椒RAPD系统的建立及去杂种子纯度鉴定的应用.园艺学报,2001,28(1):77～79
[13]刘杰,刘公社,李殿荣,等.RAPD方法鉴定油菜“杂油59”种子纯度的研究[J].应用与环境生物学报,2000,6(6):526-529
[14]段院生,刘平武,杨光圣.甘蓝型油菜杂交种亲本指纹图谱分析[J].中国油料作物学报,2002, 24(2):5～9
[15]Willams J.G.W, kubelic A.R,.DNA polymorphisms amplified by arbitrary are useful as genetic marker. Nucleic Acids Res,1990,(18):6531～6535
[16]Vos P, Hogers R,B, leeker M,, et al. AFLP a new technique for DNA fingerprinting. Nucleic Acids Res,1995,(23):4407～4414
[17] Paran I, Michelmore R W. Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theoretical and Applied Genetics,1993,( 85):985-993
[18]文雁成,王汉中,沈金雄,等.SRAP和SSR标记构建的甘蓝型油菜品种指纹图谱比较[J].中国油料作物学报,2006,28(3):233～239
[19]雷天刚,张学昆,陆合.甘蓝型油菜黄籽品种SSR指纹图谱的构建[J].西南农业大学学报(自然科学版),2005, 27 (3) : 361～364
[20]谭君,丁仲芳,孙仕贤,等.西南常用玉米自交系SSR指纹图谱构建[J].西南农业学报,2003,16(2): 1～6
[21]关荣霞,刘燕,刘章雄,等.利用方法鉴定大豆品种纯度[J].分子植物育种,2003,3(1):357～360
[22]许占友,李磊,常汝镇,等.大豆质核互作雄性不育系核不育基因的遗传分析[J] .中国农业科学,1999 ,32(增刊) :1～8
[23]苏顺宗,黄玉碧,杨俊品,等.利用SSR鉴定水稻杂交种子纯度的研究[J].种子, 2003, (1): 23～25
[24]梅德圣,李云昌,胡琼,等.甘蓝型油菜中油杂8号种子纯度的SSR鉴定[J].中国农学通报,2006,22,49～52
[25]Vandberg. J.L. Biochemical markers and restriction fragment length polymorphisms in baboons : Paternity in Primates: Genetic Test and Theories-implications of Human DNA Fingerprinting 1992.
[26]陈家宽,杨继主编.植物进化生物学[M].武汉大学出版社, 1994,153～194
[27]王中仁编,植物等位酶分析[M].北京:科学出版社, 1996,69～73
[28]Gooke R J, Gel electrophoresis for the identification of plant variety. Journal of chromatography A, 1995,698:281～229
[29]胡国之.金针茹品系间的酯酶同工酶标记筛选研究[J].生物学杂志,1999,16(1):13～14
[30]谢宗铭.生化指纹在向日葵育种上的应用L同工酶的研究和应用.作物杂志, 1999,69(2):1～4
[31]Gooke R J. Handbook of variety testing[J].ISTA,Swizterland,1992
[32] Bury W J,Bino R J.ISTA/LAHS Symposium: Technological Advances in Variety and Seed Testing. Wageningen, The Netherlands,1994,36～70
[33]于新艳,王凤格,赵久然等.SSR标记及其在玉米研究中的应用[J].安徽农业科学,2007,35(7):3
[34]宇文强.贵州杂交玉米纯度检验及其指纹图谱的构建:[硕士学位论文].云南:贵州大学,2006
[35]孔祥彬.利用SSR指纹技术构建玉米品种指纹图谱及遗传多样性研究:[硕士学位论文].山东:山东农业大学,2006
[36]李根英, Susanne Dreisigacker,Marilyn,等.小麦指纹图谱数据库的建立及SSR分子标记试剂盒的研发[J] .作物学报, 2006, 132(112): 1771-177
[37]李伟,郑有良,魏育明,等.小麦新品种川农16的分子鉴定[J].麦类作物学报, 2004,24(1):6-10
[38]李莉,杨剑波,Machill D.J.,Colow it P.M..水稻SSR不同检测和分析方法的比较研究[J].中国水稻科学, 2000,14(3):185-188
[39]PlieskeJ.,Struss D.,2001,Microsatellite markers for genome analysis in Brassica.I.developmentin Brassica napus and abundance in Brassicaceae species,Theor.App1.Genet.,102(4):689-694
[40]王风格,赵久然,郭景伦,等.中国玉米新品种标准DNA指纹库构建研究的几点思考[J].植物学通报,2005, 22(1): 121-128
[41]Provan J., Powell W. ,and Waugh R.,1996,Microsatellite analysis of relationship swith in cultivated potato (Solanumtuberosum), Theor.App1.Genet.,92(8):1078-1084
[42]王俊芳.棉花品种指纹图谱构建及棉种鉴定技术研究[J].2009, 36(3)∶6~24
[43]崔艳华,邱丽娟,常汝镇,等.利用SSR分子标记检测黄淮夏大豆初选核心样本的代表性[J].植物遗传资源学报, 2003,4(1):9-15
[44]王玺,张宝石.玉米种子纯度鉴定技术研究进展[J].种子,2002,1:43～44
[45]富昊伟.利用芽鞘及不完全叶颜色的差别鉴定水稻特定杂交组合种子纯度[J].杂交水稻,2002,17(4):27
[46]李名胜,朱宗同.几个西瓜群体材料的叶形变异与杂种纯度鉴定[J].安徽农业科学,2001,29(2),213～216
[47]马志虎,颜素芳,沈晓昆,等.辣椒黄绿苗突变体叶色标记技术在种子生产中的作用[J].种子世界,2001,9:19～20
[48]崔洪昌、郑晓鹰.大白菜种子蛋白分离的高效液相色谱法的研究[J].种子,1993,(4):7～11
[49]崔洪昌,郑晓鹰.高效液相色谱法在大白菜种子品种鉴定中的应用[J].种子,1993,5:1～3
[50]文方德,李卓杰,傅家瑞.种子纯度鉴定技术进展及其评论[J].种子,1995,5:36-38
[51]颜启传.作物品种纯度检验的进展[J].种子,1984(2):61
[52]颜启传,黄亚军.农作物品种鉴定手册[M].北京:中国农业出版社,1996
[53] O.ulvin,Handbook of seed Testing一Testing for Genuineness of Cultivar[J]. ISTA Secretary,1973
[54] Laser K.D.,Lersten N R. Anatomy and cytology of microspore genesis in cytoplasmic male sterile angiosperms [J]. Bot Rev,1972,38:427～454
[55]余凤群,傅廷栋.甘蓝型油菜几个雄性不育系花药发育的细胞形态学研究[J].武汉植物学研究,1990,3(3):209～215
[56]危文亮,王汉中,刘贵华.甘蓝型油菜细胞质雄性不育系NCa花药发育的细胞学观察[J].中国农业科学,2005,38(6):1232～1237
[57]杨光圣,瞿波,傅廷栋.三个甘蓝型油菜隐性细胞核雄性不育系小孢子发生的细胞学研究[J].华中农业大学学报,1999,18(6):520～523
[58]杨光圣,瞿波,王淑新,等.一个甘蓝型油菜DGCMS雄性不育系的花器形态及细胞学研究[J].中国油料作物学报,2000,22(3):16～21
[59]袁本威.甘蓝型油菜胞质雄性不育的细胞学观察[J].陕西农业科学,1991,(2):8～9
[60]黄邦全.甘蓝型油菜雄性不育材料S90-8-7的获得及细胞学研究[J].湖北大学学报(自然科学版),2000,22(2):182～184
[61]官春云,李栒，王国槐等.化学杂交剂诱导油菜雄性不育机理的研究.I.杀雄剂1号对甘蓝型油菜花药绒毡层和花粉粒形成的影响[J].作物学报,1997,(5):513～521
[62]李红兵.陕油8号种子纯度鉴定:[硕士学位论文].陕西:西北农林科技大学, 2005
[63]李淑娟.种子纯度鉴定方法概述[J].青海大学学报,2003,21(l)16～19
[64]Cooke J.杜充译.鉴定农作物栽培品种的电泳方法[J].国外遗传与育种.1985,1:14-20
[65]郭艳.陕油10号种子纯度鉴定: [硕士学位论文].陕西:西北农林科技大学, 2007
[66]汤陵华.亚洲主要稻作国家栽培稻在同工酶顶点的差异[J].作物学报,1991,17(6):9-16
[67]邵继荣,何清海,张述华,等.水稻多用恢复系130的七种同工酶研究[J].西南农业学报,1999,12(1):8-13
[68]岳平,宋希云,张爱民,等.用SDS-PAGE方法进行杂交小麦纯度鉴定[J].北京农业大学学报,1993,(9):59.
[69]王景升,王玺,刘国奇,等.电泳谱带法在作物品种纯度鉴定上的应用研究[J].沈阳农业大学学报,1996,(3):221-225.
[70]周展明.玉米蛋白质凝胶电泳法鉴定品种纯度的研究[J].种子世界,1992,(1):16-18.
[71]李殿荣,夏永真,王保仁.利用酯酶同工酶谱分析鉴定杂交油菜秦油二号种子纯度的方法[J].陕西农垦科技,1994,(24):10～12.
[72]梅德圣,李云昌,李应德,等.用酯酶同工酶和AFLP标记鉴定甘蓝型油菜“中油杂2号”的种子纯度[J].江西农业大学学报,(27): 1.
[73]漆小泉,徐光,郭家珍等.用α-淀粉酶同工酶分析测定中椒6号种子的品种纯度[J].中国蔬菜,1994,(2):9～10.
[74]马德伟,黄锁柱,孙岚,等.辣椒遗传性的种子醇溶蛋白电泳研究[J].园艺学报,1995,2(23):297～298.
[75]胡辉林,党润盈.方法鉴定杂交油葵及其2亲本品种纯度的研究[J].中国油料作物学报,2002, 24(2):26～28.
[76]段维,谢宗铭,陈福隆,等.利用种子蛋白丙烯酰胺凝胶电泳鉴定向日葵种子纯度的研究[J].种子,2001,(1):24～25.
[77]Anisimova,I.N, Gavrilyuck IP, Konarev CG. Identification of sunflower lines and varieties by helianthemion electrophoresis[J].PlantVarieties&Seeds,1991,(4):133～141.
[78]Varier A, Dadlani M, Cooke RJ. Identification of sunflower hybrids and inbreeds by SDS-PAGE of the seed proteins[J].Seed Research,1992,20(2):138～141.
[79]赵建宗,付友兰.水溶蛋白电泳鉴定杂交油葵种子纯度[J].种子科技,2003,21(5):284～285.
[80]纪颖彪,朱其杰.同工酶分析在黄瓜杂种一代纯度检测上的应用研究[J].园艺学报,1995,22(3):250～255.
[81]郑晓鹰,李丽,李秀清.大白菜品种同工酶及水溶蛋白的遗传多样性[J].园艺学报,1997,24(3):244～248.
[82]文方德,李卓杰,傅家瑞.用ACP及ADH同工酶等电聚焦快速鉴定番茄杂种纯度[J].园艺学报,1996,23(2):202～204.
[83]Tanksley S.D. , Jones R.A. .Application of ADH allozymes in testing the genetic purity of F1 hybrids of tomato. Hort [J].Science,1981,16(2):179～181
[84]李丽,郑晓鹰,邢宝田.蛋白电泳技术在F1代杂交种纯度鉴定中的应用[J].种子检验,2000,18:51-53
[85]彦启传.种子检验的原理和技术[M],北京:农业出版社,1992
[86]陆士伟,黄炳权,邝章标.应用酯酶同工酶测定杂交水稻杂种纯度的研究[J].中国农业科学,1982,5:10～16
[87]李卓杰,傅家瑞等.电聚焦电泳对杂交水稻种子纯度鉴定的技术[J].种子,1991,(3):68
[88]玉米种子盐溶蛋白电泳鉴定方法,NY/T449-2001[S].农业部,2001.
[89]Anisimova,I.N, Roger,J., Fido., et al. Variation and polymorphism of 2S Albumins of sunflower [J].Euphytica,1995,( 83):15～23.
[90]李殿荣,夏永真,王保红.利用酸酶同工酶谱分析鉴定杂交油菜秦油8号种子纯度的方法[J].陕西农垦科技,1994,24(杂交油菜专辑):10～12
[91]穆建新.利用分子标记技术分析鉴定杂交油菜种子纯度研究:[硕士学位论文].陕西:西北农林科技大学, 2005
[92]贾继增.分子标记种质资源鉴定和分子标记育种[J].中国农业科学, 1996,29(4):1～10
[93]方宣钧,吴为人,唐纪良.作物DNA标记辅助育种[M].北京:科学出版社, 2001.
[94]胡胜武,赵惠贤,路明.分子标记在芸荃属植物遗传育种中的应用[J].中国油料作物学报,2001,23(4)82～86
[95]刘春吉,王明利.RFLP分析过程中应注意的几个问题[J].遗传,1994,16(6):37～40.
[96]张修军.微生物融合重组子的鉴定—DNA分子检测技术[1J.生物学杂志,1999,16(2):5～7.
[97]刘旭.遗传标记和遗传图谱构建[J].作物品种资源,1997,(3):29～32.
[98]吴平.应用RFLP标记分析水稻株高与分蘖的遗传相关性[J].中国科学(C辑), 1996,26(3):257-263
[99]宣朴,瞿世洪.植物育种中的RFLP技术[J].西南农业学报,1994,7(2):106～112
[100]Sasaki T, Abstact of plant GenomeⅢ[M], San Diego USA, 1995,11
[101]Song Y C, The physical location of 14 RFLP markers in rice, Theory Appl Genet, 1995,90(1):113～119
[102]Gale M D, Genetic maps of hexaploid wheat,proc.8th,interm,wheat Genet samp, beijing, 1993,29～31
[103]Peterson L.Genetic diversity among wild and cultivated barley as revealed by RFLP. Theory Appl Genet,1989,(6):679～681
[104]Graner A, Jaboor A, schondelmaier J. construction of an RFLP map of barley, Theory Appl Genet, ,1991,83(2):250～256
[105]Keim P, Bearis W D, Schupp J M. RFLP analysis of soybean breeding populations: I. Genetic structure differences due to inbreeding methods. Crop Sci,1994,( 34):55～61.
[106]Mansur L M. Determining the linkage of qualitative trait loci to RFLP markers using extreme phenotypes of recombinant inbreds of soybean. Theory Appl Genet, 1993,86(8):914～918.
[107]Leon A J. Use of RFLP markers for genetic linkage analysis foil percentage in sunflower seed. Group Sci,1995,35(2):558～564
[108]sarfatti M, katan J, Fluhr R. A RFLP markers in tomato linked to the fusarium axysporum resistance gene 12,Theory Appl Genet, 1989,78(5):755～759
[109]Gebardt C., Blomendahl C., Schachtschabeo U., et.al. Identification of 2n breeding lines and 4n varieties of potato (Sloane tuberosum, ssp. tuberosum) with RFLP fingerprinting[J].Theory ApplGenet,1989,( 78):16～22
[110]田志宏,郑用琏,孟金陵.几个甘蓝型油菜细胞质雄性不育系线粒体DNA的RFLP分析[J].遗传,1998,20(增刊):22～26
[111]Welsh J., Mcclelland M., Finger Printing genomes using PCR with arbitrary primers. Nuel Acids Des.1990,18(24):7213～7218
[112]Sun G L, Fahima T, kord A B. Identification of molecular markers linksed to the Yr stripe rest resistance gene of wheat originated in wild emmer wheat, Triticum dicoccoises, Theory Appl Genet, 1997,(95):622～628;
[113]Vierling R A, Nguyen. Use of RAPD markers to determine the genetic diversity of diploid, wheat genotypes, Theory Appl Genet,1993,84(7-8):835～838
[114]张秀英,葛春民,贾继增.山羊草属五个基本基因组RAPD分析[J],植物学报,1999,41(4),393～397
[115]夏光敏,向凤宁,周爱芬.1999,小麦与高兵草属间体细胞胞杂交获可育杂种植株[J],植物学报,41(4),349～352
[116]Welsh.J.,Honeycutt R J,Mcdelland M. Percentage deternimation in maize hybrids using the arbitrarily primed polymerase chain reaction. Theory Appl Genet.82(4):573～576
[117]Vierling R A. Genetic diversity among elite sorghum lines revealed by restriction fragment length polymorphisms and random amplified polymorphic DNAs. Theory Appl Genet, 1994,87(7):816～820
[118]Yu L X, Nguyen. Genetic variation detected with RAPD markers among uplang ang lowland rice cultivars. Theory Appl Genet, 1992 ,87(6):668～672
[119]朱诚,刘之燕.RAPD技术与桂花品种分类和鉴定[J].广西植物, 1999,19(2):190～192
[120]Marshall P, Marchand M C, Lbieczko Z. A simple method to estimate the percentage ofhybridity in canola(Brassica napus)F1 hybrids. Theory Appl enet,1994,(98):853～858
[121]李小彬,周小梅等.水稻三系及其杂种的RAPD指纹图谱分析[J].农业生物技术学报,1995,3(2):1～6
[122]杨蜀岚,伏健民,杨仁崔,等.矮64S为不育系的两系杂交稻纯度的RAPD鉴定[J].福建农业大学学报,1998,27(1):6～9
[123]陈忠明,王秀娥,赵彦,等.两优培九种子纯度的RAPD快速鉴定.杂交水稻,2003,18(4):55～56
[124]钱前,陈洪,孙家修,等.真假杂交水稻n优63的ARPD鉴定[J].中国水稻科学.1996,10(4):241～242.
[125]李文杉,周小梅.水稻三系及其杂种的ARPD指纹图谱分析[J].农业生物技术学会,1995,3(2):l～6
[126]扬剑波,李莉等.利用ARPD技术检测杂交水稻种子纯度[J].安徽农业科学,1996,24(3):193～195.
[127]徐鑫,刘学群,瞿波,等.ARPD标记鉴定水稻3种不同细胞质雄性不育类型的杂交种及其亲本[J].遗传,2005,27(3):377～381.
[128]吴敏生,戴景瑞,王守才.RAPD在玉米品种鉴定和纯度分析中的应用[J].作物学报,1999,25(4):89～493,
[129]王凤格,赵久然,郭景伦,等.中国玉米新品种DNA指纹库建立系列研究[J],玉米科学,2003,11(2):3～6
[130]Marshall P,Marchand M C,Lbieczko Z.A simple method to estimate the percentage of hybridity in canola(Brassica napus)F1 hybrids.Theor Appl enet,1994,98:853～858,
[131]Mail R.J, Scarth R. Discrimination among cultivars of rapeseed(bressica.napus)using DNA polymorphism amplified from arbitrary primers. Theory Appl Genet,1994,( 87):694～704
[132]赵洪锟,李启云,王玉民,等.RAPD标记在大豆品种鉴定中的应用初探[J].吉林农业科学,1998(4):6～8
[133]易成新,张元真.分子标记用于棉花杂交种种子纯度测验的初步研究[J].棉花学报,1999,11(6):318～320
[134]庄木,王晓武,杨丽梅,等.利用RAPD方法鉴定两个甘蓝品种的纯度[J].中国蔬菜,1999(5):8～9
[135]范丙友,刘玉梅,高水平.RAPD技术在我国主要蔬菜遗传育种研究中的应用[J].中国蔬菜,2002(3):55～56
[136]郑晓鹰,Klocke E..利用RAPD分子标记对番茄杂交种纯度的鉴定研究[J],广西植物,2003,23(2):149～154
[137]刘富中,刘万勃,宋明,等.利用RAPD快速鉴定甜瓜杂交种的遗传纯度[J],中国蔬菜,2002,(5):7～9
[138]柳李旺,龚义勤,雷春,等.辣椒F1杂种遗传纯度的种子蛋白、同工酶与RAPD鉴定[J].分子植物育种,2003,1(5/6):663～667
[139]欧阳新星,许勇,张海英,等.应用RAPD技术快速进行西瓜杂交种纯度鉴定研究[J],农业生物技术学报,1999,7(1):23～28
[140]周群初,何清,马艳青,等.利用RAPD技术辣椒杂种纯度鉴定的研究[J].湖南农业科学,1999(5):14～15
[141]蒲汉丽,李乃坚,黄爱兴,等.RAPD技术鉴定番茄一代杂种奥星种子纯度的研究[J].种子,2000(5):14～16
[142]李成伟,李卓杰.用RAPD方法鉴定杂交辣椒种子纯度[J].种子, 1999(2): 5-6
[143]李传勇,黄聪丽,潘爱民,等.利用RAPD分析鉴定花椰菜杂种纯度[J].亚热带植物科学,2001,3(04):1～4
[144]孙敏,齐爱民,王和勇,等.黄瓜杂交种子纯度的RAPD鉴定[J].西南师范大学学报,2003,28(1)
[145]Crockett P.A, Bhalla P.L., Lee C.K., ea tl. RAPD analysis of seed purity in a commercial hybrid cabbage(Brassica oleracea var.captitata)cultivar[J].Genome,2000,( 43):317～321
[146]Hasgizume T., Sato T., Hirai M. Determination of genetic purity of hybrid seed in watermelon(Citrulluslanatus)andTomato(Lycopersiconesculentum)usingRAPD.Japan.J.Breed.1993,( 43):367～375
[147]Philllip A. Crockett,Prem L. Bhalla,O.K. RAPD analysis of seed purity in a commercial hybrid cabbage(Brasslca oleracea var. capitata)cultivar,Genome.2000,43(2):317～321
[148]邵启明,裴炎,侯磊,等.汕优63的DNA分子标记及种子纯度鉴定研究[J].西南农业大学学报,1996,18,(2)
[149]莫结胜,刘杰,刘公社.杂交油葵A15种子纯度的RAPD鉴定[J].作物学报,2001, 27,( 1):85～90
[150]Hu J., Quiros C.F. Identification of broccoli and cauliflower cultivars with RAPD markers, Plantcell Report,1991,(10):505～511
[151]Choe Y W, Park D Y, Suh K H. Pepper(Capsicum annuum L)cultivar identifacation and seed purity test by random amplified polymorphic DNAs (RAPDs)and phosphoglucomutase(PGM) isozyme, RDA Journal of Horticulture Science, 1998,40(1):15～23
[152]Meng X.D, Ma H, Zhang W.H. A fast procedure for genetic purity determination of head Chinese cabbage hybrid seed based on RAPD markers, Seed Sci.&Technol.,1998,26:829～833
[153]栾雨时,苏乔,李海涛,等.利用RAPD技术快速鉴定番茄杂种纯度[J],园艺学报,1998,25(3):247～250
[154]邓义才,王得元,李乃坚,等.利用RAPD技术鉴定早青三号黄瓜种子纯度的研究[J].广东农业科学,1999,(3):17～18
[155]李红兵,董振生,陈毓荃,等.陕油8号种子纯度的ARPD鉴定研究[J].西北植物学报,2004,24(11):2007～2011.
[156]张修军.微生物融合重组子的鉴定—DNA分子检测技术.生物学杂志, 1999,16(2):5～7
[157]王苏建,随机扩增DNA多态性及其在微生物分型中的作用.国外医学(微生物分册),1999,22(2):18～20
[158]张恒庆,红松RAPD实验中各组成份含量对实验结果的影响,植物研究,1999,19(2):183～188
[159]Tautz D. Hyper variability of simple sequences as a general source for polymorphic DNA markers[J]Nucleic Acids Research,1989,(17):6463-6471.
[160]Litt M,Luty J A.A hypervariable microsatellite revealed by invitro amplification of dinucleotide repeat within the cardiac muscle actln gene[J].American Journal of HumanGenetics,1989,(44):397-401 .
[161]李汝玉,杨平平,宋国安,等.简单序列重复(SSR)及其在品种鉴定及种子检测中的应用[J].种子,1999(5):33-35.
[162]Tautz D, Renz M. Simple sequences are ubiquitous repetitive components of eukaryotic genomes[J]Nucleic Acids Research,1984, (12):4127-4138.
[163]张立荣,徐大庆,刘大群. SSR和ISSR分子标记及其在植物遗传育种研究中的应用[J].河北农业大学学报,2002,25(1):90-94
[164]万平,刘大钧等.SSR标记与植物遗传育种研究[J].安徽农业大学学报,1998,25(1):92-95
[165]Gregan J. The Abstract of Molecular Biology symposium. Amsterdam, The Netherland, 1994,19～46
[166]辛景树,郭景伦,张软斌.几种常用分子标记技术在种子纯度和品种真实性鉴定方面的比较与分析[J].种子,2005.24(1):58-60
[167]BellCJ,EekenJR. Assignment of 30 micresatellite loci to the linkage map of Arabidopsis [J].Genomics,1994,19:137～144
[168]Wangz,WeberJL,ZhongG,etal. Survey of Plant short natdem DNA erpeat [J].Theory APPL Genet,1994,(88):1～6
[169]李文涛,张桂权.水稻微卫星标记的发展和应用[J].生命科学,2000,12(5) :234～237
[170]赵勇,杨凯,翁跃进.利用水稻功能基因SSR标记鉴定水稻种质资源[J].中国农业科学,2002,35(4):349～353
[171]李永强,李宏伟,高丽锋,等.基于表达序列标签的微卫星标记[J].植物遗传资源学报,2004,5(l):91～95
[172]番兴明,张世煌,谭静,等.根据SSR标记划分优质蛋白玉米自交系的杂种优势群[J].作物学报,2003,29(l):105～110
[173]刘世建,荣廷昭,杨俊品,等.四川地方玉米种质的SSR聚类分析[J].作物学报,2004,30(3):221～22
[174]高闻伟,李晓辉,田清震.利用SSR标记快速鉴定玉米杂交种农大108和豫玉27的种子纯度[J].种子,2004,23(5):6
[175]刘峰,陈受宜.大豆基因组中的微卫星标记[J].大豆科学,1998,17(3):256～261
[176]许占友,邱丽娟,常汝镇,等.利用SSR标记鉴定大豆种质[J].中国农业科学,1999,32(增刊:)40-48.
[177]关荣霞,刘燕,刘章雄,等.利用SSR方法鉴定大豆品种纯度[J].分子植物育种,2003,(1)3:357～360
[178]武耀廷,张天真,郭旺珍,等.陆地棉品种SSR标记的多态性及用于杂交种纯度检测的研究]JI.棉花学报,2001,13(3):13～133
[179]刘列钊,林呐.油菜简单重复序列ssR(simplesequenceerpeat)研究进展[J].生命科学,2004,16(3):173～176
[180]詹庆才.利用微卫星DNA标记进行杂交水稻种子纯度鉴定的研究[J].杂交水稻,2002,17(5):46～50
[181]李稳香,詹庆才.杂交水稻种子纯度SSR指纹图谱标记鉴定技术研究[J].中国种业,2006(3):30～31
[182]王凤格,赵久然.中国玉米新品种DNA指纹库建立系列研究Ⅲ.多重PCR技术在玉米SSR引物扩增中的应用[J].玉米科学,2003,11(4):3-6
[183]谭振馨,李汝玉,李群,等.玉米杂交种SSR标记纯度鉴定方法研究[J].山东农业科学,2004,(4):8～11
[184]高文伟,李晓辉,田清震,等.利用SSR标记快速鉴定玉米杂交种农大108和豫玉27的种子纯度[J].种子,2004(5):32～33
[185]秦利,李冰.新疆陆地棉SSR标记指纹图谱构建和杂种纯度鉴定研究[J].新疆农业科学,2005,42(6):399～401
[186]石海波,王立新等.利用SSR标记区别小麦品种种子混杂和SSR位点不纯的研究[J].分子植物育种,2006,(4):513～519
[187]艾呈详,陆璐.SSR标记技术在甜瓜杂交种纯度检测中的应用[J].园艺学报,2005,32(5):902～904
[188]刘平武,周国岭,杨光圣,等.双低甘蓝型油菜杂交种亲本指纹图谱构建和杂交种纯度鉴定[J].作物学报, 2005, 31 (5): 640-646
[189]Vos P.,Hogers R.,Zabeau M.,et al. AFLP:a new technique for DNA fingerprinting[J].Nucl Acids Res,1995,23(21):4407～4414
[190]MackillDJ. Level of Polymorphism and genetic mapping of AFLP mark esrinriee [J].Genome,1996,39(5):969～977.
[191]ChoYQBlarMW,PnaaudO·Cloning and mapping of variety一specific genomic DNA sequneees: Amplified refagmeni lenhgt polymoPhrism(SFLP)rfmosilver一stained Polyacyrlmaide gels[J].Genome,1996,(39):373～387
[192]MalleswaranM,SubudhiK,NnadiS. Polymoprhism,distribution and segregation of AFLP markers in adouble haploid rice PoPulation [J].Theory APPI Genet 1997,94(l):39～45
[193]Keim , PBearisWD , SchuPPJM.RFLP analysis of soybeen breeding populations:1.Genetic:1structure differences due to inbreeding methods[J].CorPSei,1994,(34):55～61
[194]Qix. Development of AFLP marker in barely[J].Molecular & General Genetics,1997,254(3):330～336
[195]Bachem C W B, Vander Hoeven RS, de Bruijin S M. Visualization of differentinal gene expression using a novel method of DNA fingerprinting based on AFLP, Analysis of gene expression during potato tuber development, Plant Journal, 1996,9(5),745～753
[196]尹佟明,黄敏仁. AFLP分子标记及其在植物育种上的应用[J] .生物工程进展,1997 ,17 :11～16
[197]张增翠和侯喜林.不结球白菜矮抗6号的AFLP指纹鉴定[J].江苏农业科学,2004,(1):63～64
[198]朱云国,王学德.用AFLP技术构建棉花雄性不育三系及其杂种Fl的DNA指纹图谱[J].棉花学报,2001,13(3):158～160
[199]刘杰,刘公社,齐冬梅,等.向日葵品种鉴定和纯度分析AFLP研究[]J.植物学通报,2002,19(4):444～451
[200]张峰.AFLP-银染法检测植物基因组多态性[J].细胞生物学杂志, 1999 ,21(2):98～100
[201]潘俊松,王刚,李效尊,等.黄瓜SRAP遗传连锁图的构建及始花节位的基因定位[J].自然科学进展,2005,15(2):167～172
[202]林忠旭,张献龙,聂以春。新型标记SRAP在棉花F2分离群体及吟唱多样性评价中的适用性分析[J].遗传学报,2004,31(6):622～626
[203]任羽,王得元,张银东,等.辣椒SRAP-PCR反应体系的建立与优化[J].分子植物育种,2004,2(5):689～693
[204]李严.张春庆.新型分子标记-SRAP技术体系优化及应用前景分析[J].中国农学通报,2005,5
[205]刘华,王宇生,张辉,等.中国小麦种质资源醇溶朊蛋白指纹图谱数据库的初步构建和应用[J].作物学报, 1999, 25(6): 674～682
[206]Mackill D.J..Level of polymorphism and genetic mapping of AFLP marker in rice[J].Genome,1996,39(5):969～977
[207]Zhu Z F, Sun C Q, Li Z C et al. Study on the classification of rice varieties with SSR molecular markers [J].Agric Biotech,2001,9(1):58～61
[208]王灏等,利用RAPD技术进行杂交油菜杂油59品种鉴定和纯度分析[J]中国农业科学,2002,12
[209]林道哲等,26份甘蓝型油菜自交系指纹图谱分析[J].安徽农业科学,2008,36(20)8493-8496
[210]王通强等,油菜杂种及亲本指纹图谱构建和杂种纯度鉴定[J].食品与生物技术学报, 2009,28(3)377-384
[211]Saghai-Maroof M A, Soliman K M, Jorgenson R A, Allard R W. Ribosomal DNA spacer-length polymorphisms in barley. Mendelian inheritance, chromosomal location, and populaion dynamics[J]. Proc Natl Acad Sci, 1984, 81: 8014-8018.
[212]Tautz D. Hypervariability of simple sequence as general souce for polymorphic DNA markers[J]. Nucleic Acids Res, 1989, 12: 6463-6467.
[213] BRANT JB,GUSTAVOCA,PETERMG. Fast and sensitive silver Staining of DNA in polyacrylamide gels[J].Anal Biochem.1991,19(6):80-83
[214]李进波.分子标记在水稻抗性育种及杂交稻种子纯度和真伪鉴定中的应用: [硕士毕业论文].武汉:中国农业科学院,2005
[215]苏顺宗.利用SSR分子标记鉴定杂交稻种子纯度的研究:[硕士毕业论文].四川:四川大学,2003