农药残留检测的荧光和化学发光免疫分析方法研究
|
摘要
在课题组现有ELISA分析方法的研究基础上,进一步深化研究方法的相关影响因素,如基质效应、漂移现象及不同ELISA分析方法等。在ELISA三种包被模式研究中,结果显示包被抗体法和包被二抗法在分析方法的方法灵敏度和数据平行性方面较包被抗原法更有优势。为建立具更高灵敏度且可商品化开发的免疫分析方法,本文对时间分辨荧光分析方法(TRFIA)和化学发光酶免疫分析方法(CLEIA)进行了系统的研究。
通过对TRFIA分析方法荧光增强液中螯合剂β-NTA浓度、TOPO浓度及pH值等条件的筛选,得到了对于Eu~(3+)和Sm~(3+)这两种稀土离子具有良好性能的荧光增强液。在此基础上,通过对包被温度和时间、包被缓冲液、封闭物质、反应缓冲液等条件的优化。在优化基础上,建立了克百威的TRFIA分析方法并进行了标样添加回收率。结果表明,所建立的克百威TRFIA分析方法的检测灵敏度为1.86 ng/mL,样品添加回收率试验表明建立的克百威TRFIA分析方法可用于样品中克百威残留检测。
通过对CLEIA化学发光增敏液中缓冲体系、发光增强剂、鲁米诺浓度及氧化剂浓度的筛选,得到具有良好性能的发光增敏液。在此基础上,通过对化学发光板、包被温度和时间、包被缓冲液、反应缓冲液及有机溶剂与浓度等条件的优化,结果显示:Costar白色发光板的吸附效果较佳,克百威抗体选择用0.1M,pH7.2Tris-HCl液作为包被缓冲液并在并在37℃包被两小时效果为佳,封闭物质选用1%胰蛋白胨(Tyrtone)且反应缓冲液选择0.05 M,pH7.2Tris-HCl液可得到最好的反应灵敏度和最大发光计数,有机溶剂中甲醇对CLEIA影响最小,反应体系中最终含量2.5%为佳。在此基础上,建立了克百威的CLEIA分析方法,其检测限(IC_(10))为0.362 ng/mL,检测灵敏度(IC_(50))为4.244 ng/mL,并通过LC-MS/MS数据确证。结果表明克百威CLEIA分析方法在灵敏度方面明显优于ELISA分析方法,并在样品检测中得到很好的应用,对多种样品的检测限已达到相关国家及CAC等设定的克百威MRL值。
在构建成功化学发光免疫分析系统基础上,进一步将CLEIA分析方法应用到三唑磷、毒死蜱和对硫磷三种有机磷农药残留分析技术中,建立的三唑磷、毒死蜱和对硫磷三种有机磷农药的CLEIA分析方法IC_(50)值分别为0.852、21.01和2.247 ng/mL,IC_(10)值分别为0.063、0.694和0.203 ng/mL。并分别与三种农药的ELISA分析方法的检测灵敏度和检测限进行比较,结果显示,CLEIA分析方法的检测限均有了较大的提高,特别是三唑磷的CLEIA分析方法,相比于ELISA分析方法其检测灵敏度和检测限提高了2个数量级,显示出CLEIA分析方法在农药残留检测方面应用的良好前景。
On the basis of the existing research of the author's laboratory about the ELISA method,in-depth study about the effect of various factors which including matrix effect,drift phenomenon and different ELISA coating formats to ELISA method was investigated.Comparison of different ELISA formats showed that antibody coated format and the second antibody coated format had better performance in sensitivity and variation coefficient of determination than antigen coated format.In order to establish the immuno-chemical method which had higher sensitivity and good application prospect,the systematic experiments about time-resolved fluorescence immunoassay(TRFIA) and chemiluminescence ELISA immunoassay(CLEIA) were carried out.
A variety of conditions of fluorescence enhancement solution including the concentration ofβ-NTA,concentration of TOPO and pH value were optimized, subsequently,the fluorescence enhancement solution which had good enhancement activity to both Eu~(3+) and Sm~(3+) was obtained.Based on the results,TRFIA conditions were optimized.On the basis of the optimized conditions mentioned above,the carbofuran TRFIA method was generated and recoveries of standards was determined. The sensitivity for detection,the IC_(50) value was 38.55 ng/ml at a practical working concentration range from 1.86 to 798.8 ng/ml,and the limit of detection for carbofuran was 1.86 ng/ml.The mean variation coefficient of standard determination was 25.30%.Continued,analysis of carbofuran in spiked sample was finished. Unfortunately,there was no advantage about the carbofuran TRFIA method to the carbofuran ELISA method.Therefore,the research on carbofuran CLEIA method was carried out.
The chemiluminescence sensitizer was obtained after a variety of conditions were optimized.On the basis of it,CLEIA method conditions for earbofuran was optimized and the optimum conditions were given as followed:Costar~(?) white plate for chemiluminescence was coated with the antibody in 0.1M,pH7.2 Tris-HC1 and the plate was incubated at 37℃for 2 hours.Also,1%typtone as blocking substance and 0.05 M,pH7.2Tris-HCl buffer as reacting buffer could improve the method's sensitivity and get the largest light-emitting count.And 2.5%methanol was suitable for the competitive reaction.On the basis of optimum conditions,the carbofuran CLEIA method was generated successfully.The sensitivity for detection,the IC_(50) value was 4.244 ng/ml at a practical working concentration range from 0.362 to 49.76 ng/ml and the limit of detection for carbofuran was 0.362 ng/ml.Recoveries of carbofuran spiked into vegetable samples and environmental samples were determined by CLEIA after sample matrix effect testing.Finally,the confirmation test between CLEIA and LC-MS/MS was finished.The results showed that CLEIA method had higher sensitivity than ELISA method,and the limit of detection of a variety of samples reached the carbofuran's MRL value laid down by CAC and relevant countries.
On the success in generating the chemiluminescence immunoassay system,the analytical method was applied in determination of organophosphorus pesticides including triazophos,chlorpyrifos and parathion.The IC_(50) value of CLEIA methods in determination of triazophos,chlorpyrifos and parathion were 0.852,21.01 and 2.247ng/mL,anad IC_(10) value were 0.063,0.694 and 0.203ng/mL respectively. Subsequently,the results were compared with the results of ELISA methods.It showed that the LOD of CLEIA methods have been greatly improved,especially the triazophos CLEIA method.Compared to the ELISA method,the detection sensitivity and LOD were improved two orders of magnitude.It indicated that the CLEIA method had good prospects of application in detection of pesticide residue.
通过对TRFIA分析方法荧光增强液中螯合剂β-NTA浓度、TOPO浓度及pH值等条件的筛选,得到了对于Eu~(3+)和Sm~(3+)这两种稀土离子具有良好性能的荧光增强液。在此基础上,通过对包被温度和时间、包被缓冲液、封闭物质、反应缓冲液等条件的优化。在优化基础上,建立了克百威的TRFIA分析方法并进行了标样添加回收率。结果表明,所建立的克百威TRFIA分析方法的检测灵敏度为1.86 ng/mL,样品添加回收率试验表明建立的克百威TRFIA分析方法可用于样品中克百威残留检测。
通过对CLEIA化学发光增敏液中缓冲体系、发光增强剂、鲁米诺浓度及氧化剂浓度的筛选,得到具有良好性能的发光增敏液。在此基础上,通过对化学发光板、包被温度和时间、包被缓冲液、反应缓冲液及有机溶剂与浓度等条件的优化,结果显示:Costar白色发光板的吸附效果较佳,克百威抗体选择用0.1M,pH7.2Tris-HCl液作为包被缓冲液并在并在37℃包被两小时效果为佳,封闭物质选用1%胰蛋白胨(Tyrtone)且反应缓冲液选择0.05 M,pH7.2Tris-HCl液可得到最好的反应灵敏度和最大发光计数,有机溶剂中甲醇对CLEIA影响最小,反应体系中最终含量2.5%为佳。在此基础上,建立了克百威的CLEIA分析方法,其检测限(IC_(10))为0.362 ng/mL,检测灵敏度(IC_(50))为4.244 ng/mL,并通过LC-MS/MS数据确证。结果表明克百威CLEIA分析方法在灵敏度方面明显优于ELISA分析方法,并在样品检测中得到很好的应用,对多种样品的检测限已达到相关国家及CAC等设定的克百威MRL值。
在构建成功化学发光免疫分析系统基础上,进一步将CLEIA分析方法应用到三唑磷、毒死蜱和对硫磷三种有机磷农药残留分析技术中,建立的三唑磷、毒死蜱和对硫磷三种有机磷农药的CLEIA分析方法IC_(50)值分别为0.852、21.01和2.247 ng/mL,IC_(10)值分别为0.063、0.694和0.203 ng/mL。并分别与三种农药的ELISA分析方法的检测灵敏度和检测限进行比较,结果显示,CLEIA分析方法的检测限均有了较大的提高,特别是三唑磷的CLEIA分析方法,相比于ELISA分析方法其检测灵敏度和检测限提高了2个数量级,显示出CLEIA分析方法在农药残留检测方面应用的良好前景。
On the basis of the existing research of the author's laboratory about the ELISA method,in-depth study about the effect of various factors which including matrix effect,drift phenomenon and different ELISA coating formats to ELISA method was investigated.Comparison of different ELISA formats showed that antibody coated format and the second antibody coated format had better performance in sensitivity and variation coefficient of determination than antigen coated format.In order to establish the immuno-chemical method which had higher sensitivity and good application prospect,the systematic experiments about time-resolved fluorescence immunoassay(TRFIA) and chemiluminescence ELISA immunoassay(CLEIA) were carried out.
A variety of conditions of fluorescence enhancement solution including the concentration ofβ-NTA,concentration of TOPO and pH value were optimized, subsequently,the fluorescence enhancement solution which had good enhancement activity to both Eu~(3+) and Sm~(3+) was obtained.Based on the results,TRFIA conditions were optimized.On the basis of the optimized conditions mentioned above,the carbofuran TRFIA method was generated and recoveries of standards was determined. The sensitivity for detection,the IC_(50) value was 38.55 ng/ml at a practical working concentration range from 1.86 to 798.8 ng/ml,and the limit of detection for carbofuran was 1.86 ng/ml.The mean variation coefficient of standard determination was 25.30%.Continued,analysis of carbofuran in spiked sample was finished. Unfortunately,there was no advantage about the carbofuran TRFIA method to the carbofuran ELISA method.Therefore,the research on carbofuran CLEIA method was carried out.
The chemiluminescence sensitizer was obtained after a variety of conditions were optimized.On the basis of it,CLEIA method conditions for earbofuran was optimized and the optimum conditions were given as followed:Costar~(?) white plate for chemiluminescence was coated with the antibody in 0.1M,pH7.2 Tris-HC1 and the plate was incubated at 37℃for 2 hours.Also,1%typtone as blocking substance and 0.05 M,pH7.2Tris-HCl buffer as reacting buffer could improve the method's sensitivity and get the largest light-emitting count.And 2.5%methanol was suitable for the competitive reaction.On the basis of optimum conditions,the carbofuran CLEIA method was generated successfully.The sensitivity for detection,the IC_(50) value was 4.244 ng/ml at a practical working concentration range from 0.362 to 49.76 ng/ml and the limit of detection for carbofuran was 0.362 ng/ml.Recoveries of carbofuran spiked into vegetable samples and environmental samples were determined by CLEIA after sample matrix effect testing.Finally,the confirmation test between CLEIA and LC-MS/MS was finished.The results showed that CLEIA method had higher sensitivity than ELISA method,and the limit of detection of a variety of samples reached the carbofuran's MRL value laid down by CAC and relevant countries.
On the success in generating the chemiluminescence immunoassay system,the analytical method was applied in determination of organophosphorus pesticides including triazophos,chlorpyrifos and parathion.The IC_(50) value of CLEIA methods in determination of triazophos,chlorpyrifos and parathion were 0.852,21.01 and 2.247ng/mL,anad IC_(10) value were 0.063,0.694 and 0.203ng/mL respectively. Subsequently,the results were compared with the results of ELISA methods.It showed that the LOD of CLEIA methods have been greatly improved,especially the triazophos CLEIA method.Compared to the ELISA method,the detection sensitivity and LOD were improved two orders of magnitude.It indicated that the CLEIA method had good prospects of application in detection of pesticide residue.
引文
1.袁增雯,张一宾.农药与环境[J].上海化工,2000,17:4-5.
2.赵善欢主编.植物化学保护(第三版)[M].北京:中国农业出版社,1999:P1.
3.Yang S.S.,Huang C.B.,Smetena 1.Optimization of headspace sampling using Solid-phase microextraction for volatile components in tobacco[J].Journal of Chromatography Analysis,2001,(1):33-39.
4.Millan S.,Sampedro M.,Unceta N.,et al.Coupling solid-phase microextraction and high performance liquid chromatography for direct and sensitive determination of halogenated fungicides in wine[3]Journal of Chromatography A,2003,995(1-2):135-142.
5.Otero R.,Grande B.,Gandara J.Multiresidue method for fourteen fungicides in white grapes by liquid-liquid and solid-phase extraction followed by liquid chromatography-diode array detection[J]Journal of Chromatography A,2003,992(1-2):121-131.
6.Schenck F.J.,Donoghue D.J.Determination of organochlorine and organophosphorus pesticide residues in eggs using a solid phase extraction cleanup[J].Journal of Agricultural and Food Chemistry,2000,48(12):6412-641.
7.贺德春,杨仁斌,龚道新,等.用气相色谱法测定面粉和大米中14种农药残留量[J].湖南农业大学学报(自然科学版),2004,30(2):161-164。
8.Linkerhagner M.,Stan H.J.Screening analysis of pesticide residues in plant foodstuffs by capillary gas chromatography using the DFG multiresidue method S19:a comparison of customary detection by ECD/NPD with the novel atomic emission detector(AED)[J].Z Lebensm Unters Forsch,1994,198(6):473-479.
9.Eisert R.,Levsen K.Determination oforganophosphorus,triazine,and 2,6- dinitroanalyine Pesticides in aqueous samples via SPME and GC with NPD detection[J1.Fresenius Journal of A nalytical Chemistry,1995:555-562.
10.Obana H,Okihashi M,Akutsu K,et al.Determination ofneonicotinoid pesticide residues in vegetables and fruits with solid phase extraction and liquid chromatography mass spectrometry[J].Journal of Agricultural and Food Chemistry,2003,51(9):2501-2505.
11.Charlton A.,Stuckey V.,Sykes M.Determination of the Phenoxyacid Herbicides MCPA,Mecoprop and 2,4-D in Kidney Tissue Using Liquid Chromatography with Electrospray Tandem Mass Spectrometry[J]Bulletin of Environmental Contamination and Toxicology,2009,82(6):711-715.
12.Kinseila B.,Lehotay S.,Mastovska K.,et al.New method for the analysis of flukicide and other anthelmintic residues in bovine milk and liver using liquid chromatography-tandem mass spectrometry[J].Analytica Chimica Acta,2009,637(1-2):196-207.
13.Saito Y.,Kodama S.,Matsunaga A.,et al.Multiresidue determination of pesticides in agricultural products by gas chromatography / mass spectrometry with large volume injection[J].The Journal of AOAC International,2004,87(6):1356-1367.
14.Stan H.J.Pesticide residue analysis in foodstuffs applying capillary gas chromatography with mass spectrometric detection[J].Journal of Chromatography Analysis.2000.892(l-2):347-377.
15.Kusakable T.,Saito T.,Takeichi S.Solid-phase microextraction and gas chromatography-mass spectrometry analysis of P,P-DDE in biological samples[J].Journal of Chromatography B:Biomedical Sciences and Application,2001,(1):93-98.
16.Wong J.W.Webster M.G,Halverson C.A.,et al.Multiresidue pesticide analysis in wines by solid-phase extraction and capillary gas chromatography-mass spectrometric detection with selective ion monitoring[J].Journal of Agricultural and Food Chemistry,2003,51(5):1148-1161.
17.Song Y.,Ge Y.,Zhang Y.,et al.Hapten synthesis and enzyme-linked immunosorbent assay for phosmet residues:assay optimization and investigation of matrix effects from different food samples[J],Analytical and Bioanalytical Chemistry,2009,393(8):2001-2008.
18.Abad,A.,Moreno,M.J.,& Montoya,A.A monoclonal immunoassay for carbofuran and its application to the analysis of fruit juices.Analytica Chimica Acta,1997,347(1-2):103-110.
19.Munro,C.,& tabenfeldt,S.G.Development of a Microtitre Plate Enzyme Immunoassay for the Determination of Progesterone,Journal of Endocrinology,1984,101(1):41-49.
20.Zeng,K.,Yang,T.B.,Zhong,P.,et al.Development of an indirect competitive immunoassay for parathion in vegetables,Food Chemistry,2007,102:1076-1082.
21.Watanabe E.,Baba K.,Eun H.,et al.Application of a commercial immunoassay to the direct determination of insecticide imidacloprid in fruit juices[J].Food Chemistry,2007,102(3):745-750.
22.Li Z.,Liu Y.,Sun Y,et al.Development of polyclonal antibody based enzyme-linked immunosorbent assay for the analysis of the agricultural insecticide imidacloprid:food quality and safety[J].Asia pacific journal of clinical nutrition,2007,16:102-105.
23.Anand C,Pasha A.,Swetha M.,et al.Development of polyclonal ELISA for the N-methylcarbamate insecticide bendiocarb[J].Food and Agricultural Immunoassay,2007,18:39-51.
24.Moreno M.,Plana E.,Montoya A.,et al.Application of a monoclonal-based immunoassay for the determination of imazalil in fruit juices[J].Food Additives and Contaminants,2007,24(7):704-712.
25.Kim B.,Eiaty A.,Hwang T.,et al.Development of an acetylcholinesterase-based detection kit for the determination of organophosphorus and carbamate pesticide residues in agricultural samples[J].Bulletin of the Korean Chemical Society,2007,28(6):929-935.
26.Coons A.H.,Creech H.J.,Jones R.N.,et al.The Demonstration of Pneumococcal Antigen in Tissues by the Use of Fluorescent Antibody[J].The Journal of Immunology,1942,45:159-170.
27.李勇,何俊,但德忠等.异硫氰酸荧光素标记人血清白蛋白[J].华西药学杂志,2001,16(1):25-26.
28.贾春平,赵建龙.免疫标记技术的进展及纳米技术在其中的应用[J].生命科学,2008,20(5):749-753.
29.金晶,赖卫华,涂祖新等.时间分辨荧光免疫分析技术的研究进展及在食品安全领域的应用[J].食品科学,2006,27(12):886-889.
30.Aggerbeck H.,Norgaard P.,Herson Ⅰ.Simultaneous quantitation of diphtheria and tetanus antibodies by double antigen,time-resolved fluorescence immunoassay[J],Journal of Immunological Methods,1996,190(2):423-428.
31.Maple P.,Jones C.,Andrews N.Time resolved fluorometrie immunoassay,using europium labelled antihuman IgG,for the detection of human tetanus antitoxin in serum[J].Journal of Clinical Pathology,2001,54(10):812-815.
32.Soukka,T.,Paukkunen,J.,Harma,H.,et al.Supersensitive time-resolved immunofluorometric assay of free prostate-specific antigen with nanoparticle label technology[J].Clinical Chemistry,2001,47(7):1269-1278.
33.Wu F.He Y.,Han S.Matrix interference in serum total thyroxin(T4) time-resolved fluorescence immunoassay(TRFIA) and its elimination with the use of streptavidin-biotin separation technique[J].Clinica ChimicaActa,2001,308(1-2):117-126.
34.黄飚.赭曲霉毒素A和黄曲霉毒素B的超微量免疫分析.江南大学博士学位论文,2006.6.
35.Meares C.,Mccall M.,Reardan D.,et al.Conjugation of antibodies with bifunctional chelating agents isothioeynate and bromoacetamide reagents,methods of analysis,and subsequent addition of metal ions[J].Analytical Biochemistry,1984,142(1):68-78.
36.Degan P.,Abbondandolo A.,Montagnoli G.,et al.A new fluorescence enhancement solution for Europium-based time-resolved fluoroimmunoassays[J].Journal of Bioluminescence and Chemiluminescence,1990,5(3):207-212.
37.Chan M.,Bellern A.,Diamandis E.,et al.Time-resolved immunofluorometric assay of alpha-fetoprotein in serum and amniotic fluid,with a novel detection system[J],Clinical Chemistry,1987,33:2000-2003
38.Barnard G.,Kohen E,Mikola H.,et al.Measurement of estrone-3-glucuronide in urine by rapid,homogeneous time-resolved fluoroimmunoassay[J]Clinical Chemistry,1989,35:555-559.
39.李振甲,陈泮藻,高平等编著,时间分辨荧光分析技术与应用[M].北京:科学出版社,1996:P26-27.
40.何明祥.沙丁胺醇时间分辨荧光免疫分析方法的研究.福建农林大学硕士学位论文,2008.4.
41.张祯.动物组织中氯霉素和莱克多巴胺残留的时间分辨荧光免疫检测方法的研究.中国农业大学硕士学位论文,2006.5.
42.潘利华,安锦龙,谢文兵等.稀土螯合物BCPDA-Eu~(3+)标记蛋白质研究[J].光谱学与光谱分析,2004,24(7):795-798.
43.Bailey M.,Bernard F.,Rocks,et al.Terbium chelate for use as label in fluorescent immunoassays[J].Analyst,1984,109:1449-1450.
44.Diamandis E.P.,Christopoulos T.K.Europium chelate labels in time-resolved fluorescence immunoassay and DNA hybridization assays[J].Analytical Chemistry,1990,62(22):1149-1157.
45.薛钢,雷红涛,吴青等,荧光偏振免疫分析技术研究进展[J].食品工业科技,2008,3:289-292.
46.朱广华,郑洪,鞠熀先.荧光偏振免疫分析技术的研究进展[J].分析化学,2004,32(1):102-106.
47.Margaret L.,Glenn W.,Michael E.,et al.Fluorescence polarization immunoassay Ⅳ.Determination of phenytoin and phenobarbital in human serum and plasma[J].Clinical Chemistry,1982,28(11):2278-2282.
48.Julia N.,Anna Y.,Ekaterina A.,et al.Express detection of nonylphenol in water samples by fluorescence polarization immunoassay[J].Analytical and Bioanalytical Chernistry,2004,378:634-641.
49.Shim W.,Yakovleva M.,Kim K.,et al.Development of Fluorescence Polarization Immunoassay for the Rapid Detection of 6-Chloronicotinic Acid Main Metabolite of Neonicotinoid Insecticides[J]Journal of Agricultural and Food Chemistry,2009,57(3):791-796.
50.王战辉,张素霞,沈建忠,荧光偏振免疫分析测定磺胺甲基嘧啶研究[J].光谱学与光谱分析,2008,28(7):1621-1625.
51.Hildebrandt N.,Charbonniere L.,Loehmannsroeben H.Time-resolved analysis of a highly sensitive Forster resonance energy transfer immunoassay using Terbium complexes as donors and quantum dots as acceptors[J].Journal of Biomedicine and Biotechnology,2007,2007:1-6.
52.Valanne A.,Malmi P.,Appelblom H.,et al.A dual-step fluorescence resonance energy transfer based quenching assay for screening of caspase-3 inhibitors[J].Analytical Biochemistry,2008,375(1):71-81.
53.Liu L.,Shao M.,Dong X.Homogeneous immunoassay based on two-photon excitation fluorescence resonance energy transfer[J].Analytical Chemistry,2008,80(20):7735-7741.
54.Tinna K.,Tuomo L.,Mari T.Homogeneous Dual-Parameter Assay for Prostate-Specific Antigen Based on Fluorescence Resonance Energy Transfer[J].Analytical Chemistry,2008,80(24):9763-9768.
55.华东.一种高效安全化学发光EMSA系统的建立及应用.东南大学硕士学位论文,2007.4.
56.Halman M.,Velan B.,Sery T.Rapid identification and quantitation of small numbers of microorganisms by a chemiluminescent immunoreaction[J].Applied and Environmental Microbiology,1977,34:473-477.
57.李晓霞.化学发光免疫分析[J].延安大学学报(医学科学版),2004,2(2):51-53.
58.邵晓东.鲁米诺化学发光体系在药物和环境分析中的应用及反应机理研究.西北大学硕士学位论文,2008.5.
59.林金明主编.化学发光基础理论与应用[M].北京:化学工业出版社,2004:P41-42.
60.Weeks I.,Beheshti I.,Mcpara F.,et al.Acridinium seters sa high specific activity labels in immunoassay IJ].Clinical Chemistry.1983.29:1474-1479.
61.王晓娟,漆红兰,张成孝.利用吖啶酯化学发光研究甲醛和乙醛对DNA的损伤[J].陕西师范大学学报(自然科学版),2006,34(3):73-76.
62.尹东光,贺佑丰,刘一兵等,几种主要化学发光物质的发光性能及其化学发光免疫分析体系[J].I临床免疫分析与临床,2002,9(4):225-230.
63.林斯.氯霉素酶促化学发光免疫分析方法的建立.中国原子能科学研究院硕士学位论文,2005.7.
64.林金明主编.化学发光免疫分析[M].北京:化学工业出版社,2008:P21.
65.Leland J.,Powell M.Electrogenerated Chemiluminescence:An Oxidative-Reduction Type ECL Reaction Sequence Using Tripropyl Amine[J].Journal of the Electrochemical Society, 137(10):3127-3131.
66.张允平,单汉青,徐龙宝等,电化学发光免疫分析法测定TSH、FT3、FT4的临床应用[J].标记免疫分析与临床,2000,7(2):109-111
67.綦玉芹,王娈,电化学发光免疫分析法测定血清胰岛素效果评价[J].青岛大学医学院学报,2002,38(4),344-345.
68.Barker M.H.,Schiffrin D.J.,Abuknesha R.Electrochemiluminescence flow injection immunoassay for atrazine.Biosensors and Bioelectron.,1997,12(4):277-286.
69.骆建新,郑崛树,王伦安等,流动注射协同增强化学发光免疫分析法的建立与评价[J].分析化学,2002,30(7):792-796.
70.King J.,Desai U.A capillary electrophoretic method for fingerprinting low molecular weight heparins[J].Analytical Biochemistry,2008,380:229-234.
71.郭小明,胡涌刚,徐向东等.毛细管电泳-化学发光法检测废水中的铜离子[J]化学与生物工程,2007,24(2):69-72.
72.吴永平,高智席,周光明.HPLC-CL测定吊石苣苔中石吊兰素的含量[J]西南师范大学学报(自然科学版),2007,32(3):48-51.
73.Jose Fernando H.P.,Ana Maria G.C.Determination of N-methylcarbamate pesticides in water and vegetable samples by HPLC with post-column chemiluminescence detection using the luminol reaction[J].Analytica Chimica Acta,2008,630:194-204.
74.Subiela S.M.,Eckersall P.D.,Campbell F.M.,et al.A time-resolved immunofluorometric assay for porcine C-reactive protein quantification in whole blood[J].Luminescence,2007,22:171-176.
75.Siitari H.,Hemmila E.,Soini E.,et al.Detection of hepatitis B surface antigen using time-resolved fluoroimmunoassay[J].Nature,1983,301(20):258-260.
76.Yan F.,Zhou J.,Lin J.,et al.Flow injection immunoassay for carcinoembryonic antigen combined with time-resolved fluorometric detection[J].Journal of Immunological Methods,2005,305:120-127.
77.Matsumoto K.,Yuan J.,Wang G,.et al.Simultaneous Determination of α-Fetoprotein and Carcinoembryonic Antigen in Human Serum by Time-Resolved Fluoroimmunoassay[J].Analytical Bochemistry,1999,276:81-87.
78.Valanne A.,Malmi P.,Appelblom H.,et al.A dual-step fluorescence resonance energy transfer-based quenching assay for screening of caspase-3 inhibitors[J].Analytical Biochemistry,2008,375:71-81.
79.Bao S.,Peng Y.,Sheng S.,et al.Assessment of Urinary Total Testosterone Production by a Highly Sensitive Time-Resolved Fluorescence Immunoassay[J].Journal of clinical laboratory analysis,2008,22(6):403-408.
80.蔡卫民,陈杰,陈刚.淬灭荧光免疫法测定微量血清中庆大霉素的浓度[J].中国医院药学杂志,1991,9(8):485-486.
81.Margaret L.,Glenn W.,Michael E.,et al.Fluorescence polarization immunoassay Ⅳ.Determination of phenytoin and phenobarbital in human serum and plasma[J].Clinical Chemistry,1982,28(11 ):2278-2282.
82.Bacigalupo M.A.,Meroni G.,Secundo F.,et al.Time-resolved fluoroimmunoassay for quantitative determination of ampicillin in cow milk samples with different fat contents[J].Talanta,2008,77:126-130.
83.Wang Z.,Zhang S.,Murtazina N.,et al.Determination of the veterinary drug maduramicin in food by fluorescence polarisation immunoassay[J].International Journal of Food Science and Technology,2008,43(1):114-122.
84.Zhao Y.,Liang Y.,Qian J.,et al.Determination of Diethylstilbestrol by Time-resolve Fluoroimmunoassay[J].Analytical Letters,2009,42(2):216-227.
85.Yakovleva J.N.,Lobanova A.Y.,Shutaleva E.A.,et al.Express detection of nonylphenol in water samples by fluorescence polarization immunoassay[J].Analytical and Bioanalytical Chemistry,2004,378(3):634-641.
86.Halman M.,Velan B.,Sery T.Rapid identification and quantitation of small numbers of microorganisms by a ehemiluminescent immunoreaction[J].Applied and Environmental Microbiology,1977,34:473-477.
87.Wang J.,Ren J.A sensitive and rapid immunoassay for quantification of CAl25 in human sera by capillary electrophoresis with enhanced chemiluminescence detection[J].Electrophoresis,2005,26,2402-2408.
88.Liu Y.M.,Mu H.B.,Zheng Y.L.,et al.Capillary electrophoretic immunoassay for alpha-fetoprotein with chemiluminescence detection[J].Journal of Chromatography B,2007,855:280-285.
89.Zhang Q.,Wang X.,Li Z,.et al.Evaluation of alpha-fetoprotein(AFP) in human serum by chemiluminescence enzyme immunoassay with magnetic particles and coated tubes as solid phases[J].Analytica Chimica Acta,2009,631:212-217.
90.Zhan W.,Bard A.J.,Electrogenerated Chemiluminescence.83.Immunoassay of Human C-Reactive Protein by Using Ru(bpy)_3~(2+)-Eneapsulated Liposomes as Labels[J].Analytical Chemistry,2007,79:459-463.
91.Knight C.S.,Crum M.A.,Hardy R.W.Evaluation of the LIAISON Chemiluminescence Immunoassay for Diagnosis of Syphilis[J].Clinic and Vaccine Immunology,2007,14(6): 710-713.
92.Perschel F.,Schemer R.,Seiler L.,et al.Rapid Screening Test for Primary Hyperaldosteronism:Ratio of Plasma Aldosterone to Renin Concentration Determined by Fully Automated Chemiluminescence Immunoassays[J].Endocrinology and Metabolism,2004,50(9):1650-1655.
93.Tudorache M.,Zdrojewska I.,Emneus J.,et al.Evaluation of progesterone content in saliva using magnetic particle-based immuno supported liquid membrane assay(m-ISLMA)[J].Biosensors and Bioelectronics,2006,22:241-246.
94.Iwata R.,Hayashi T,Nakao Y.,et al.Direct determination of plasma endothelin-I by chemiluminescence enzyme immunoassay[J].Clinical Chemistry,1996,42(8):1155-1158.95.Qi H.,Zhang C.Homogeneous electrogenerated chemiluminescence immunoassay for the determination of digoxin[J].Analytica Chimica Acta,2004,501:31-35.
96.Zhou M,Guan C,Chen G,et al.Determination of theophylline concentration in serum by chemiluminescent immunoassay[J].Journal of Zhejiang Universitu Science B,2005,6(12):1148-1152.
97.Peter L.,Christine B.,Ingrid S.,et al.7α-Biotinylated testosterone derivatives as tracers or a competitive chemiluminescence immunoassay of testosterone in serum[J].Clinical chemistry,1997,43(12):2345-2352.
98.Mirasoli M.,Deo S.K.,Lewis J.C.,et al.Bioluminescence Immunoassay for Cortisol Using Recombinant Aequorin as a Label[J].Analytical Biochemistry,2002,306:204-211.
99.Luo L.,Zhang Z.,Hou L.,et al.The study of a chemiluminescence immunoassay using the peroxyoxalate chemiluminescent reaction and its application[J].Talanta,2007,72:1293-1297.
100.Li M.,Yoshida H.,Aramak Y,et al.Improved enzyme immunoassay for human basic fibroblast growth factor using a new enhanced chemiluminescence system[J].Biochemical and Biophysical research communications.1993,193(2):540-545.
101.Luo L.,Zhang Z.,Ma L.,et al.Determination of recombinant human tumor necrosis factor-a in serum by chemiluminescence imaging[J].Analytica Chimica Acta,2005,539:277-282.
102.Quan Y.,Zhang Y.,Wang S.,et al.A rapid and sensitive chemiluminescence enzyme-linked immunosorbent assay for the determination of fumonisin Bl in food samples[J].Analytica ChimicaActa,2006,580:1-8.
103.Yang M.,Kostov Y.,Bruck H.,et al.Carbon Nanotubes with Enhanced Chemiluminescence Immunoassay for CCD-Based Detection of Staphylococcal Enterotoxin B in Food[J].Analytical Chemistry,2008,80:8532-8537.
104.Lin S.,Han S.,Liu Y.,et al.Chemiluminescence immunoassay for chloramphenicol[J].Analytical and Bioanalytical Chemistry,2005,382:1250-1255.
105.杨秀岑,伍莉萍,李军等,食品中沙门氏菌的化学发光免疫快速检验[J].华西医学杂志,1991,6(1):4-6.
106.Magliulo M.,Mirasoli M.,Simoni P.,et al.Development and Validation of an Ultrasensitive Chemiluminescent Enzyme Immunoassay for Aflatoxin MI in Milk[J].Journal of Agricultural and Food Chemistry,2005,53:3300-3305.
107.Kijek T.,Rossi C.A.,Moss D.,et al.Rapid and sensitive immunomagnetic electrochemiluminescent detection of staphyloccocal enterotoxin B[J].Journal of Immunological Methods,2000,236:9-17.
108.Christian S.,Heinz F.S.,Rudolf J.S.Direct sub-ppt detection of the endocrine disruptor ethinylestradiol in water with a chemiluminescence enzyme-linked immunosorbent assay [J].Analytica Chimica Aata,2005,551:92-97.
109.Pittrnan T.L.,Thomson B.,Miao W.Ultrasensitive detection of TNT in soil,water,using enhanced electrogenerated chemiluminescence[J].Analytica Chimica Acta,2009,632:197-202.
110.Zhao L.,Lin J.Development of a micro-plate magnetic chemiluminescence enzyme immunoassay(MMCLEIA) for rapid-and high-throughput analysis of 17β-estradiol inn water samples[J].Journal of biotechnology,2005,118:177-186.
111.Alexandra E.,Sergei A.,Angel M.,et al.Development of chemiluminescent ELISAs to DDT and its metabolites in food and environmental samples[J].Journal of Immunological Methods,2003,283(1):45-57.
112.David J.B.,Nichola A.P.,Felicity A.R.A fast screening method for the presence of atrazine and other triazines in water using flow injection with ehemiluminescent detection[J]Talanta,2009,78:342-347.
113.Samaonova J.V.,Rubtsova M.Y.,Kiseleva A.V.,et al.Chemiluminescent multiassay of pesticides with horseradish peroxide as a label[J].Biosensors & Bioelectronics,1999,14:273-281.
114.Waseem A.,Yaqoob M.,Nabi A.Photodegradation and flow-injection determination of simetryn herbicide by luminol chemiluminescence detection[J].Analytical sciences,2008,24(8):979-983.
115.Kim H.J.,Shelver W.L.,Hwang E.C.Automated flow fluorescent immunoassay for pat per trillion detection of the neonicotinoid insecticide thiamethoxam[J].Analytica Chiraica Acta, 2006,571:66-73.
116.Kolosova A.Y.,Park J.Ho,Eremin A.E.,et al.Comparative study of three immunoassays based on monoclonal antibodies for the detection of the pesticide parathion-methyl in real samples[J].Analytica Chimica Acta,2004,511:323-331.
117.Deryabina MoA.,Yakovleva J.N.,Popova V.A.,et al.Determination of the herbicide acetochlor by fluorescence polarization immunoassay[J].Journal of Analytical Chemistry,2005,60(1):80-85.
118.Bacigalupo M.,Meroni G.Quantitative determination of diuron in ground and surface water by time-resolved fluoroimmunoassay Seasonal variations of diuron,carbofuran,and paraquat in an agricultural area[J].Journal of Agricultural and Food Chemistry,2005,55:3823-3828.
119.Jin,R.Y.,Wu,J.X.,Zhu,G.N.,et al,Development of the Monoclonal Antibody to Carbofuran,Chinese Journal of Agro-Environmental Science,2006,25(5):1276-1280.
120.朱国念,杨挺,吴银良.抗克百威多克隆抗体的研制[J].中国农业科学,2002,35(8):1025-1029.
121.Zhu G.,Jin M.,Gui W.,et al.Development of a direct competitive enzyme-linked immunoassay for carbofuran in vegetables,Food Chemistry,2008,107(4 ):1737-1742
122.Skerritt,J.H.,Guihot,S.L.,Asha,M.B.Sensitive immunoassays for methyl-parathion and parathion and their application to residues in foodstuffs[J].Food Agricultural Immunology,2003,15,1-15.
123.Meyer H.,Guven,B.Improvement of Microtitration Plate Enzyme Immunoassays for Steroid Determination by a Second Antibody Technique,Journal of Steroid Biochemistry,Supplement,1986,25:139.
124.Munro,C.,tabenfeldt,S Development ofa Microtitre Plate Enzyme Immunoassay for the Determination of Progesterone[.1],Journal of Endocrinology,1984,101(1):41-49.
125.郭周义,田振,贾雅丽.时间分辨免疫分析方法的光谱研究[J].光谱学与光谱分析,2004,24(5):596-599.
126.Shen J.,Zhang Z.,Yao Y.,et al.A monoclonal antibody-based time-resolved fluoroimmunoassay for chloramphenicol in shrimp and chicken muscle[J].Analytica Chimica Acta,2006,575:262-266.
127.赵启仁,李美佳,张福华.时间分辨荧光免疫分析技术研究[J].医学研究通讯,2004,33(7):25-26.
128.桂文君.农药残留ELISA速测试剂盒产业化技术研究--三唑磷、克百威ELISA试剂盒研制,浙江大学博士学位论文,2007.6
129.Gui,W.J.,Jin,R.,Chen,Z.J.,et al.Hapten synthesis for enzyme-linked immunoassay of the insecticide triazophos[J],Analytical Biochemistry,2006,357(1):9-14
130.Liu Y.,Jin M.,Gui G.,et al.Hapten design and indirect competitive immunoassay for parathion determination.Correlation with molecular modeling and principal component analysis[J].Analytica Chimica Acta,2007,591:173-182
131.金仁耀,桂文君,吴建祥等.抗毒死蜱单克隆抗体的研制[J].浙江大学学报(农业与生命科学版),2006,32(6):591-597.
132.Jefferis R.,Deverill I.The antigen antibody reaction[M].Stockton Press:New York,1991:Chapter 1.
133.李喜旺,李俊锁.抗磺胺类药物抗体的研制[J]。中国农业科学,1999,32(4):79-84
134.Kramer P.,Li Q.,Hammock B.Integration of liquid chromatography with immunoassay:an approach combining the strengths of both methods[J],Journal of AOAC International,1994,77(5):1275-1287.
135.Casino,P.,Morais,S.,Puchades,R.,et al.Evaluation of enzyme-linked immunoassays for the determination of chloroacetanilides in water and soils[J].Environmental Science &Technology.2001,35:4111-4119.
136.Harrison,R.O.,Brimfield,A.A.,and Nelson,J.O.Development of a monoclonal antibody based enzyme immunoassay method for analysis of maleic hydrazide[J].Journal of Agriculture and Food Chemistry.1989,37:958-964.
137.Lee,J.K.,Ahn,K.C.,Park,O.S.,et al.and Hammock,B.D.Development of an ELISA for the detection of the residues of the insecticide imidacloprid in agricultural and environmental.Journal of Agriculture and Food Chemistry.2001,49:2159-2167.
138.Li,Q.X.,Zhao,M.S.,Gee,S.J.,et al.Seiber,J.N.,and Hammock,B.D.Development of enzyme-linked immunosorbent assays for 4-nitrophenol and substituted 4-nitrophenois.Journal of Agriculture and Food Chemistry.1991,39:1685-1692.
139.Mancl(?)s,J.J.,Montoya,A.Development of enzyme-linked immunosorbent assays for the insecticide ehlorpyrifos.2.assay optimization and application to environmental waters.1996,Journal of Agriculture and Food Chemistry.44:4063-4070.
140.王鸿梅,冯静.韭菜挥发油中化学成分的研究[J].天津医科大学学报,2002,8(2):191-192
141.张玲,时延增,徐新刚,等.韭菜子中氨基酸及微量元素分析[J].时珍国药研究,1996:7(1):21.
142.王春梅,程敬丽,刘毅华等.拟除虫菊酯类农药酶免疫分析方法研究进展[J].农药学学报,2008,10(1):15-22.
143.Miyake S.,Beppu R.,Yamaguchi Y,et al.Polyclonal and monoclonal antibodies specific to the chrysathemic acid moiety of pyrethroid insecticides[J].Pesticide science,1998,54:189-194.
144.Watanabe T.,Shan G.M.,Stoutamire D.W.,et al.Development of a class-specific immunoassay for the type Ⅰ pyrethroid insecticides[J].Analytica Chimica Acta,2001,444:119-129.
145.Mak S.K.,Shan G.M.,Lee H.J.,et al.Development of a class selective immunoassay for the typeⅡ pyrethroid insecticides[J].Analytica Chimica Acta,2005,534:109-120.
146.金仁耀.抗克百威-三唑磷双特异性单克隆抗体研究,浙江大学博士学位论文,2008.6.
147.Soini,E.,Lovgren,T.Time-resolved fluorescence of lanthanide probes and applications in biotechnology[J].Analytical Chemistry,1997,18:105-154.
148.Margaret H.,Robert S.Enhanced chemilunescent reation and disgnostic[P].GB 2205945A.
149.Masato S.,Hu M.,Kevan L.Photolonization of alkylphen othiazines in vesicles:effects of the alkyl chain length and the vesicle surface charge[J].Physical.Chemistry,1990,94:870-874.
150.Davis P.,Henigan D.,Hines K.Assay of peroxidase activity[P].US6432662B1.
2.赵善欢主编.植物化学保护(第三版)[M].北京:中国农业出版社,1999:P1.
3.Yang S.S.,Huang C.B.,Smetena 1.Optimization of headspace sampling using Solid-phase microextraction for volatile components in tobacco[J].Journal of Chromatography Analysis,2001,(1):33-39.
4.Millan S.,Sampedro M.,Unceta N.,et al.Coupling solid-phase microextraction and high performance liquid chromatography for direct and sensitive determination of halogenated fungicides in wine[3]Journal of Chromatography A,2003,995(1-2):135-142.
5.Otero R.,Grande B.,Gandara J.Multiresidue method for fourteen fungicides in white grapes by liquid-liquid and solid-phase extraction followed by liquid chromatography-diode array detection[J]Journal of Chromatography A,2003,992(1-2):121-131.
6.Schenck F.J.,Donoghue D.J.Determination of organochlorine and organophosphorus pesticide residues in eggs using a solid phase extraction cleanup[J].Journal of Agricultural and Food Chemistry,2000,48(12):6412-641.
7.贺德春,杨仁斌,龚道新,等.用气相色谱法测定面粉和大米中14种农药残留量[J].湖南农业大学学报(自然科学版),2004,30(2):161-164。
8.Linkerhagner M.,Stan H.J.Screening analysis of pesticide residues in plant foodstuffs by capillary gas chromatography using the DFG multiresidue method S19:a comparison of customary detection by ECD/NPD with the novel atomic emission detector(AED)[J].Z Lebensm Unters Forsch,1994,198(6):473-479.
9.Eisert R.,Levsen K.Determination oforganophosphorus,triazine,and 2,6- dinitroanalyine Pesticides in aqueous samples via SPME and GC with NPD detection[J1.Fresenius Journal of A nalytical Chemistry,1995:555-562.
10.Obana H,Okihashi M,Akutsu K,et al.Determination ofneonicotinoid pesticide residues in vegetables and fruits with solid phase extraction and liquid chromatography mass spectrometry[J].Journal of Agricultural and Food Chemistry,2003,51(9):2501-2505.
11.Charlton A.,Stuckey V.,Sykes M.Determination of the Phenoxyacid Herbicides MCPA,Mecoprop and 2,4-D in Kidney Tissue Using Liquid Chromatography with Electrospray Tandem Mass Spectrometry[J]Bulletin of Environmental Contamination and Toxicology,2009,82(6):711-715.
12.Kinseila B.,Lehotay S.,Mastovska K.,et al.New method for the analysis of flukicide and other anthelmintic residues in bovine milk and liver using liquid chromatography-tandem mass spectrometry[J].Analytica Chimica Acta,2009,637(1-2):196-207.
13.Saito Y.,Kodama S.,Matsunaga A.,et al.Multiresidue determination of pesticides in agricultural products by gas chromatography / mass spectrometry with large volume injection[J].The Journal of AOAC International,2004,87(6):1356-1367.
14.Stan H.J.Pesticide residue analysis in foodstuffs applying capillary gas chromatography with mass spectrometric detection[J].Journal of Chromatography Analysis.2000.892(l-2):347-377.
15.Kusakable T.,Saito T.,Takeichi S.Solid-phase microextraction and gas chromatography-mass spectrometry analysis of P,P-DDE in biological samples[J].Journal of Chromatography B:Biomedical Sciences and Application,2001,(1):93-98.
16.Wong J.W.Webster M.G,Halverson C.A.,et al.Multiresidue pesticide analysis in wines by solid-phase extraction and capillary gas chromatography-mass spectrometric detection with selective ion monitoring[J].Journal of Agricultural and Food Chemistry,2003,51(5):1148-1161.
17.Song Y.,Ge Y.,Zhang Y.,et al.Hapten synthesis and enzyme-linked immunosorbent assay for phosmet residues:assay optimization and investigation of matrix effects from different food samples[J],Analytical and Bioanalytical Chemistry,2009,393(8):2001-2008.
18.Abad,A.,Moreno,M.J.,& Montoya,A.A monoclonal immunoassay for carbofuran and its application to the analysis of fruit juices.Analytica Chimica Acta,1997,347(1-2):103-110.
19.Munro,C.,& tabenfeldt,S.G.Development of a Microtitre Plate Enzyme Immunoassay for the Determination of Progesterone,Journal of Endocrinology,1984,101(1):41-49.
20.Zeng,K.,Yang,T.B.,Zhong,P.,et al.Development of an indirect competitive immunoassay for parathion in vegetables,Food Chemistry,2007,102:1076-1082.
21.Watanabe E.,Baba K.,Eun H.,et al.Application of a commercial immunoassay to the direct determination of insecticide imidacloprid in fruit juices[J].Food Chemistry,2007,102(3):745-750.
22.Li Z.,Liu Y.,Sun Y,et al.Development of polyclonal antibody based enzyme-linked immunosorbent assay for the analysis of the agricultural insecticide imidacloprid:food quality and safety[J].Asia pacific journal of clinical nutrition,2007,16:102-105.
23.Anand C,Pasha A.,Swetha M.,et al.Development of polyclonal ELISA for the N-methylcarbamate insecticide bendiocarb[J].Food and Agricultural Immunoassay,2007,18:39-51.
24.Moreno M.,Plana E.,Montoya A.,et al.Application of a monoclonal-based immunoassay for the determination of imazalil in fruit juices[J].Food Additives and Contaminants,2007,24(7):704-712.
25.Kim B.,Eiaty A.,Hwang T.,et al.Development of an acetylcholinesterase-based detection kit for the determination of organophosphorus and carbamate pesticide residues in agricultural samples[J].Bulletin of the Korean Chemical Society,2007,28(6):929-935.
26.Coons A.H.,Creech H.J.,Jones R.N.,et al.The Demonstration of Pneumococcal Antigen in Tissues by the Use of Fluorescent Antibody[J].The Journal of Immunology,1942,45:159-170.
27.李勇,何俊,但德忠等.异硫氰酸荧光素标记人血清白蛋白[J].华西药学杂志,2001,16(1):25-26.
28.贾春平,赵建龙.免疫标记技术的进展及纳米技术在其中的应用[J].生命科学,2008,20(5):749-753.
29.金晶,赖卫华,涂祖新等.时间分辨荧光免疫分析技术的研究进展及在食品安全领域的应用[J].食品科学,2006,27(12):886-889.
30.Aggerbeck H.,Norgaard P.,Herson Ⅰ.Simultaneous quantitation of diphtheria and tetanus antibodies by double antigen,time-resolved fluorescence immunoassay[J],Journal of Immunological Methods,1996,190(2):423-428.
31.Maple P.,Jones C.,Andrews N.Time resolved fluorometrie immunoassay,using europium labelled antihuman IgG,for the detection of human tetanus antitoxin in serum[J].Journal of Clinical Pathology,2001,54(10):812-815.
32.Soukka,T.,Paukkunen,J.,Harma,H.,et al.Supersensitive time-resolved immunofluorometric assay of free prostate-specific antigen with nanoparticle label technology[J].Clinical Chemistry,2001,47(7):1269-1278.
33.Wu F.He Y.,Han S.Matrix interference in serum total thyroxin(T4) time-resolved fluorescence immunoassay(TRFIA) and its elimination with the use of streptavidin-biotin separation technique[J].Clinica ChimicaActa,2001,308(1-2):117-126.
34.黄飚.赭曲霉毒素A和黄曲霉毒素B的超微量免疫分析.江南大学博士学位论文,2006.6.
35.Meares C.,Mccall M.,Reardan D.,et al.Conjugation of antibodies with bifunctional chelating agents isothioeynate and bromoacetamide reagents,methods of analysis,and subsequent addition of metal ions[J].Analytical Biochemistry,1984,142(1):68-78.
36.Degan P.,Abbondandolo A.,Montagnoli G.,et al.A new fluorescence enhancement solution for Europium-based time-resolved fluoroimmunoassays[J].Journal of Bioluminescence and Chemiluminescence,1990,5(3):207-212.
37.Chan M.,Bellern A.,Diamandis E.,et al.Time-resolved immunofluorometric assay of alpha-fetoprotein in serum and amniotic fluid,with a novel detection system[J],Clinical Chemistry,1987,33:2000-2003
38.Barnard G.,Kohen E,Mikola H.,et al.Measurement of estrone-3-glucuronide in urine by rapid,homogeneous time-resolved fluoroimmunoassay[J]Clinical Chemistry,1989,35:555-559.
39.李振甲,陈泮藻,高平等编著,时间分辨荧光分析技术与应用[M].北京:科学出版社,1996:P26-27.
40.何明祥.沙丁胺醇时间分辨荧光免疫分析方法的研究.福建农林大学硕士学位论文,2008.4.
41.张祯.动物组织中氯霉素和莱克多巴胺残留的时间分辨荧光免疫检测方法的研究.中国农业大学硕士学位论文,2006.5.
42.潘利华,安锦龙,谢文兵等.稀土螯合物BCPDA-Eu~(3+)标记蛋白质研究[J].光谱学与光谱分析,2004,24(7):795-798.
43.Bailey M.,Bernard F.,Rocks,et al.Terbium chelate for use as label in fluorescent immunoassays[J].Analyst,1984,109:1449-1450.
44.Diamandis E.P.,Christopoulos T.K.Europium chelate labels in time-resolved fluorescence immunoassay and DNA hybridization assays[J].Analytical Chemistry,1990,62(22):1149-1157.
45.薛钢,雷红涛,吴青等,荧光偏振免疫分析技术研究进展[J].食品工业科技,2008,3:289-292.
46.朱广华,郑洪,鞠熀先.荧光偏振免疫分析技术的研究进展[J].分析化学,2004,32(1):102-106.
47.Margaret L.,Glenn W.,Michael E.,et al.Fluorescence polarization immunoassay Ⅳ.Determination of phenytoin and phenobarbital in human serum and plasma[J].Clinical Chemistry,1982,28(11):2278-2282.
48.Julia N.,Anna Y.,Ekaterina A.,et al.Express detection of nonylphenol in water samples by fluorescence polarization immunoassay[J].Analytical and Bioanalytical Chernistry,2004,378:634-641.
49.Shim W.,Yakovleva M.,Kim K.,et al.Development of Fluorescence Polarization Immunoassay for the Rapid Detection of 6-Chloronicotinic Acid Main Metabolite of Neonicotinoid Insecticides[J]Journal of Agricultural and Food Chemistry,2009,57(3):791-796.
50.王战辉,张素霞,沈建忠,荧光偏振免疫分析测定磺胺甲基嘧啶研究[J].光谱学与光谱分析,2008,28(7):1621-1625.
51.Hildebrandt N.,Charbonniere L.,Loehmannsroeben H.Time-resolved analysis of a highly sensitive Forster resonance energy transfer immunoassay using Terbium complexes as donors and quantum dots as acceptors[J].Journal of Biomedicine and Biotechnology,2007,2007:1-6.
52.Valanne A.,Malmi P.,Appelblom H.,et al.A dual-step fluorescence resonance energy transfer based quenching assay for screening of caspase-3 inhibitors[J].Analytical Biochemistry,2008,375(1):71-81.
53.Liu L.,Shao M.,Dong X.Homogeneous immunoassay based on two-photon excitation fluorescence resonance energy transfer[J].Analytical Chemistry,2008,80(20):7735-7741.
54.Tinna K.,Tuomo L.,Mari T.Homogeneous Dual-Parameter Assay for Prostate-Specific Antigen Based on Fluorescence Resonance Energy Transfer[J].Analytical Chemistry,2008,80(24):9763-9768.
55.华东.一种高效安全化学发光EMSA系统的建立及应用.东南大学硕士学位论文,2007.4.
56.Halman M.,Velan B.,Sery T.Rapid identification and quantitation of small numbers of microorganisms by a chemiluminescent immunoreaction[J].Applied and Environmental Microbiology,1977,34:473-477.
57.李晓霞.化学发光免疫分析[J].延安大学学报(医学科学版),2004,2(2):51-53.
58.邵晓东.鲁米诺化学发光体系在药物和环境分析中的应用及反应机理研究.西北大学硕士学位论文,2008.5.
59.林金明主编.化学发光基础理论与应用[M].北京:化学工业出版社,2004:P41-42.
60.Weeks I.,Beheshti I.,Mcpara F.,et al.Acridinium seters sa high specific activity labels in immunoassay IJ].Clinical Chemistry.1983.29:1474-1479.
61.王晓娟,漆红兰,张成孝.利用吖啶酯化学发光研究甲醛和乙醛对DNA的损伤[J].陕西师范大学学报(自然科学版),2006,34(3):73-76.
62.尹东光,贺佑丰,刘一兵等,几种主要化学发光物质的发光性能及其化学发光免疫分析体系[J].I临床免疫分析与临床,2002,9(4):225-230.
63.林斯.氯霉素酶促化学发光免疫分析方法的建立.中国原子能科学研究院硕士学位论文,2005.7.
64.林金明主编.化学发光免疫分析[M].北京:化学工业出版社,2008:P21.
65.Leland J.,Powell M.Electrogenerated Chemiluminescence:An Oxidative-Reduction Type ECL Reaction Sequence Using Tripropyl Amine[J].Journal of the Electrochemical Society, 137(10):3127-3131.
66.张允平,单汉青,徐龙宝等,电化学发光免疫分析法测定TSH、FT3、FT4的临床应用[J].标记免疫分析与临床,2000,7(2):109-111
67.綦玉芹,王娈,电化学发光免疫分析法测定血清胰岛素效果评价[J].青岛大学医学院学报,2002,38(4),344-345.
68.Barker M.H.,Schiffrin D.J.,Abuknesha R.Electrochemiluminescence flow injection immunoassay for atrazine.Biosensors and Bioelectron.,1997,12(4):277-286.
69.骆建新,郑崛树,王伦安等,流动注射协同增强化学发光免疫分析法的建立与评价[J].分析化学,2002,30(7):792-796.
70.King J.,Desai U.A capillary electrophoretic method for fingerprinting low molecular weight heparins[J].Analytical Biochemistry,2008,380:229-234.
71.郭小明,胡涌刚,徐向东等.毛细管电泳-化学发光法检测废水中的铜离子[J]化学与生物工程,2007,24(2):69-72.
72.吴永平,高智席,周光明.HPLC-CL测定吊石苣苔中石吊兰素的含量[J]西南师范大学学报(自然科学版),2007,32(3):48-51.
73.Jose Fernando H.P.,Ana Maria G.C.Determination of N-methylcarbamate pesticides in water and vegetable samples by HPLC with post-column chemiluminescence detection using the luminol reaction[J].Analytica Chimica Acta,2008,630:194-204.
74.Subiela S.M.,Eckersall P.D.,Campbell F.M.,et al.A time-resolved immunofluorometric assay for porcine C-reactive protein quantification in whole blood[J].Luminescence,2007,22:171-176.
75.Siitari H.,Hemmila E.,Soini E.,et al.Detection of hepatitis B surface antigen using time-resolved fluoroimmunoassay[J].Nature,1983,301(20):258-260.
76.Yan F.,Zhou J.,Lin J.,et al.Flow injection immunoassay for carcinoembryonic antigen combined with time-resolved fluorometric detection[J].Journal of Immunological Methods,2005,305:120-127.
77.Matsumoto K.,Yuan J.,Wang G,.et al.Simultaneous Determination of α-Fetoprotein and Carcinoembryonic Antigen in Human Serum by Time-Resolved Fluoroimmunoassay[J].Analytical Bochemistry,1999,276:81-87.
78.Valanne A.,Malmi P.,Appelblom H.,et al.A dual-step fluorescence resonance energy transfer-based quenching assay for screening of caspase-3 inhibitors[J].Analytical Biochemistry,2008,375:71-81.
79.Bao S.,Peng Y.,Sheng S.,et al.Assessment of Urinary Total Testosterone Production by a Highly Sensitive Time-Resolved Fluorescence Immunoassay[J].Journal of clinical laboratory analysis,2008,22(6):403-408.
80.蔡卫民,陈杰,陈刚.淬灭荧光免疫法测定微量血清中庆大霉素的浓度[J].中国医院药学杂志,1991,9(8):485-486.
81.Margaret L.,Glenn W.,Michael E.,et al.Fluorescence polarization immunoassay Ⅳ.Determination of phenytoin and phenobarbital in human serum and plasma[J].Clinical Chemistry,1982,28(11 ):2278-2282.
82.Bacigalupo M.A.,Meroni G.,Secundo F.,et al.Time-resolved fluoroimmunoassay for quantitative determination of ampicillin in cow milk samples with different fat contents[J].Talanta,2008,77:126-130.
83.Wang Z.,Zhang S.,Murtazina N.,et al.Determination of the veterinary drug maduramicin in food by fluorescence polarisation immunoassay[J].International Journal of Food Science and Technology,2008,43(1):114-122.
84.Zhao Y.,Liang Y.,Qian J.,et al.Determination of Diethylstilbestrol by Time-resolve Fluoroimmunoassay[J].Analytical Letters,2009,42(2):216-227.
85.Yakovleva J.N.,Lobanova A.Y.,Shutaleva E.A.,et al.Express detection of nonylphenol in water samples by fluorescence polarization immunoassay[J].Analytical and Bioanalytical Chemistry,2004,378(3):634-641.
86.Halman M.,Velan B.,Sery T.Rapid identification and quantitation of small numbers of microorganisms by a ehemiluminescent immunoreaction[J].Applied and Environmental Microbiology,1977,34:473-477.
87.Wang J.,Ren J.A sensitive and rapid immunoassay for quantification of CAl25 in human sera by capillary electrophoresis with enhanced chemiluminescence detection[J].Electrophoresis,2005,26,2402-2408.
88.Liu Y.M.,Mu H.B.,Zheng Y.L.,et al.Capillary electrophoretic immunoassay for alpha-fetoprotein with chemiluminescence detection[J].Journal of Chromatography B,2007,855:280-285.
89.Zhang Q.,Wang X.,Li Z,.et al.Evaluation of alpha-fetoprotein(AFP) in human serum by chemiluminescence enzyme immunoassay with magnetic particles and coated tubes as solid phases[J].Analytica Chimica Acta,2009,631:212-217.
90.Zhan W.,Bard A.J.,Electrogenerated Chemiluminescence.83.Immunoassay of Human C-Reactive Protein by Using Ru(bpy)_3~(2+)-Eneapsulated Liposomes as Labels[J].Analytical Chemistry,2007,79:459-463.
91.Knight C.S.,Crum M.A.,Hardy R.W.Evaluation of the LIAISON Chemiluminescence Immunoassay for Diagnosis of Syphilis[J].Clinic and Vaccine Immunology,2007,14(6): 710-713.
92.Perschel F.,Schemer R.,Seiler L.,et al.Rapid Screening Test for Primary Hyperaldosteronism:Ratio of Plasma Aldosterone to Renin Concentration Determined by Fully Automated Chemiluminescence Immunoassays[J].Endocrinology and Metabolism,2004,50(9):1650-1655.
93.Tudorache M.,Zdrojewska I.,Emneus J.,et al.Evaluation of progesterone content in saliva using magnetic particle-based immuno supported liquid membrane assay(m-ISLMA)[J].Biosensors and Bioelectronics,2006,22:241-246.
94.Iwata R.,Hayashi T,Nakao Y.,et al.Direct determination of plasma endothelin-I by chemiluminescence enzyme immunoassay[J].Clinical Chemistry,1996,42(8):1155-1158.95.Qi H.,Zhang C.Homogeneous electrogenerated chemiluminescence immunoassay for the determination of digoxin[J].Analytica Chimica Acta,2004,501:31-35.
96.Zhou M,Guan C,Chen G,et al.Determination of theophylline concentration in serum by chemiluminescent immunoassay[J].Journal of Zhejiang Universitu Science B,2005,6(12):1148-1152.
97.Peter L.,Christine B.,Ingrid S.,et al.7α-Biotinylated testosterone derivatives as tracers or a competitive chemiluminescence immunoassay of testosterone in serum[J].Clinical chemistry,1997,43(12):2345-2352.
98.Mirasoli M.,Deo S.K.,Lewis J.C.,et al.Bioluminescence Immunoassay for Cortisol Using Recombinant Aequorin as a Label[J].Analytical Biochemistry,2002,306:204-211.
99.Luo L.,Zhang Z.,Hou L.,et al.The study of a chemiluminescence immunoassay using the peroxyoxalate chemiluminescent reaction and its application[J].Talanta,2007,72:1293-1297.
100.Li M.,Yoshida H.,Aramak Y,et al.Improved enzyme immunoassay for human basic fibroblast growth factor using a new enhanced chemiluminescence system[J].Biochemical and Biophysical research communications.1993,193(2):540-545.
101.Luo L.,Zhang Z.,Ma L.,et al.Determination of recombinant human tumor necrosis factor-a in serum by chemiluminescence imaging[J].Analytica Chimica Acta,2005,539:277-282.
102.Quan Y.,Zhang Y.,Wang S.,et al.A rapid and sensitive chemiluminescence enzyme-linked immunosorbent assay for the determination of fumonisin Bl in food samples[J].Analytica ChimicaActa,2006,580:1-8.
103.Yang M.,Kostov Y.,Bruck H.,et al.Carbon Nanotubes with Enhanced Chemiluminescence Immunoassay for CCD-Based Detection of Staphylococcal Enterotoxin B in Food[J].Analytical Chemistry,2008,80:8532-8537.
104.Lin S.,Han S.,Liu Y.,et al.Chemiluminescence immunoassay for chloramphenicol[J].Analytical and Bioanalytical Chemistry,2005,382:1250-1255.
105.杨秀岑,伍莉萍,李军等,食品中沙门氏菌的化学发光免疫快速检验[J].华西医学杂志,1991,6(1):4-6.
106.Magliulo M.,Mirasoli M.,Simoni P.,et al.Development and Validation of an Ultrasensitive Chemiluminescent Enzyme Immunoassay for Aflatoxin MI in Milk[J].Journal of Agricultural and Food Chemistry,2005,53:3300-3305.
107.Kijek T.,Rossi C.A.,Moss D.,et al.Rapid and sensitive immunomagnetic electrochemiluminescent detection of staphyloccocal enterotoxin B[J].Journal of Immunological Methods,2000,236:9-17.
108.Christian S.,Heinz F.S.,Rudolf J.S.Direct sub-ppt detection of the endocrine disruptor ethinylestradiol in water with a chemiluminescence enzyme-linked immunosorbent assay [J].Analytica Chimica Aata,2005,551:92-97.
109.Pittrnan T.L.,Thomson B.,Miao W.Ultrasensitive detection of TNT in soil,water,using enhanced electrogenerated chemiluminescence[J].Analytica Chimica Acta,2009,632:197-202.
110.Zhao L.,Lin J.Development of a micro-plate magnetic chemiluminescence enzyme immunoassay(MMCLEIA) for rapid-and high-throughput analysis of 17β-estradiol inn water samples[J].Journal of biotechnology,2005,118:177-186.
111.Alexandra E.,Sergei A.,Angel M.,et al.Development of chemiluminescent ELISAs to DDT and its metabolites in food and environmental samples[J].Journal of Immunological Methods,2003,283(1):45-57.
112.David J.B.,Nichola A.P.,Felicity A.R.A fast screening method for the presence of atrazine and other triazines in water using flow injection with ehemiluminescent detection[J]Talanta,2009,78:342-347.
113.Samaonova J.V.,Rubtsova M.Y.,Kiseleva A.V.,et al.Chemiluminescent multiassay of pesticides with horseradish peroxide as a label[J].Biosensors & Bioelectronics,1999,14:273-281.
114.Waseem A.,Yaqoob M.,Nabi A.Photodegradation and flow-injection determination of simetryn herbicide by luminol chemiluminescence detection[J].Analytical sciences,2008,24(8):979-983.
115.Kim H.J.,Shelver W.L.,Hwang E.C.Automated flow fluorescent immunoassay for pat per trillion detection of the neonicotinoid insecticide thiamethoxam[J].Analytica Chiraica Acta, 2006,571:66-73.
116.Kolosova A.Y.,Park J.Ho,Eremin A.E.,et al.Comparative study of three immunoassays based on monoclonal antibodies for the detection of the pesticide parathion-methyl in real samples[J].Analytica Chimica Acta,2004,511:323-331.
117.Deryabina MoA.,Yakovleva J.N.,Popova V.A.,et al.Determination of the herbicide acetochlor by fluorescence polarization immunoassay[J].Journal of Analytical Chemistry,2005,60(1):80-85.
118.Bacigalupo M.,Meroni G.Quantitative determination of diuron in ground and surface water by time-resolved fluoroimmunoassay Seasonal variations of diuron,carbofuran,and paraquat in an agricultural area[J].Journal of Agricultural and Food Chemistry,2005,55:3823-3828.
119.Jin,R.Y.,Wu,J.X.,Zhu,G.N.,et al,Development of the Monoclonal Antibody to Carbofuran,Chinese Journal of Agro-Environmental Science,2006,25(5):1276-1280.
120.朱国念,杨挺,吴银良.抗克百威多克隆抗体的研制[J].中国农业科学,2002,35(8):1025-1029.
121.Zhu G.,Jin M.,Gui W.,et al.Development of a direct competitive enzyme-linked immunoassay for carbofuran in vegetables,Food Chemistry,2008,107(4 ):1737-1742
122.Skerritt,J.H.,Guihot,S.L.,Asha,M.B.Sensitive immunoassays for methyl-parathion and parathion and their application to residues in foodstuffs[J].Food Agricultural Immunology,2003,15,1-15.
123.Meyer H.,Guven,B.Improvement of Microtitration Plate Enzyme Immunoassays for Steroid Determination by a Second Antibody Technique,Journal of Steroid Biochemistry,Supplement,1986,25:139.
124.Munro,C.,tabenfeldt,S Development ofa Microtitre Plate Enzyme Immunoassay for the Determination of Progesterone[.1],Journal of Endocrinology,1984,101(1):41-49.
125.郭周义,田振,贾雅丽.时间分辨免疫分析方法的光谱研究[J].光谱学与光谱分析,2004,24(5):596-599.
126.Shen J.,Zhang Z.,Yao Y.,et al.A monoclonal antibody-based time-resolved fluoroimmunoassay for chloramphenicol in shrimp and chicken muscle[J].Analytica Chimica Acta,2006,575:262-266.
127.赵启仁,李美佳,张福华.时间分辨荧光免疫分析技术研究[J].医学研究通讯,2004,33(7):25-26.
128.桂文君.农药残留ELISA速测试剂盒产业化技术研究--三唑磷、克百威ELISA试剂盒研制,浙江大学博士学位论文,2007.6
129.Gui,W.J.,Jin,R.,Chen,Z.J.,et al.Hapten synthesis for enzyme-linked immunoassay of the insecticide triazophos[J],Analytical Biochemistry,2006,357(1):9-14
130.Liu Y.,Jin M.,Gui G.,et al.Hapten design and indirect competitive immunoassay for parathion determination.Correlation with molecular modeling and principal component analysis[J].Analytica Chimica Acta,2007,591:173-182
131.金仁耀,桂文君,吴建祥等.抗毒死蜱单克隆抗体的研制[J].浙江大学学报(农业与生命科学版),2006,32(6):591-597.
132.Jefferis R.,Deverill I.The antigen antibody reaction[M].Stockton Press:New York,1991:Chapter 1.
133.李喜旺,李俊锁.抗磺胺类药物抗体的研制[J]。中国农业科学,1999,32(4):79-84
134.Kramer P.,Li Q.,Hammock B.Integration of liquid chromatography with immunoassay:an approach combining the strengths of both methods[J],Journal of AOAC International,1994,77(5):1275-1287.
135.Casino,P.,Morais,S.,Puchades,R.,et al.Evaluation of enzyme-linked immunoassays for the determination of chloroacetanilides in water and soils[J].Environmental Science &Technology.2001,35:4111-4119.
136.Harrison,R.O.,Brimfield,A.A.,and Nelson,J.O.Development of a monoclonal antibody based enzyme immunoassay method for analysis of maleic hydrazide[J].Journal of Agriculture and Food Chemistry.1989,37:958-964.
137.Lee,J.K.,Ahn,K.C.,Park,O.S.,et al.and Hammock,B.D.Development of an ELISA for the detection of the residues of the insecticide imidacloprid in agricultural and environmental.Journal of Agriculture and Food Chemistry.2001,49:2159-2167.
138.Li,Q.X.,Zhao,M.S.,Gee,S.J.,et al.Seiber,J.N.,and Hammock,B.D.Development of enzyme-linked immunosorbent assays for 4-nitrophenol and substituted 4-nitrophenois.Journal of Agriculture and Food Chemistry.1991,39:1685-1692.
139.Mancl(?)s,J.J.,Montoya,A.Development of enzyme-linked immunosorbent assays for the insecticide ehlorpyrifos.2.assay optimization and application to environmental waters.1996,Journal of Agriculture and Food Chemistry.44:4063-4070.
140.王鸿梅,冯静.韭菜挥发油中化学成分的研究[J].天津医科大学学报,2002,8(2):191-192
141.张玲,时延增,徐新刚,等.韭菜子中氨基酸及微量元素分析[J].时珍国药研究,1996:7(1):21.
142.王春梅,程敬丽,刘毅华等.拟除虫菊酯类农药酶免疫分析方法研究进展[J].农药学学报,2008,10(1):15-22.
143.Miyake S.,Beppu R.,Yamaguchi Y,et al.Polyclonal and monoclonal antibodies specific to the chrysathemic acid moiety of pyrethroid insecticides[J].Pesticide science,1998,54:189-194.
144.Watanabe T.,Shan G.M.,Stoutamire D.W.,et al.Development of a class-specific immunoassay for the type Ⅰ pyrethroid insecticides[J].Analytica Chimica Acta,2001,444:119-129.
145.Mak S.K.,Shan G.M.,Lee H.J.,et al.Development of a class selective immunoassay for the typeⅡ pyrethroid insecticides[J].Analytica Chimica Acta,2005,534:109-120.
146.金仁耀.抗克百威-三唑磷双特异性单克隆抗体研究,浙江大学博士学位论文,2008.6.
147.Soini,E.,Lovgren,T.Time-resolved fluorescence of lanthanide probes and applications in biotechnology[J].Analytical Chemistry,1997,18:105-154.
148.Margaret H.,Robert S.Enhanced chemilunescent reation and disgnostic[P].GB 2205945A.
149.Masato S.,Hu M.,Kevan L.Photolonization of alkylphen othiazines in vesicles:effects of the alkyl chain length and the vesicle surface charge[J].Physical.Chemistry,1990,94:870-874.
150.Davis P.,Henigan D.,Hines K.Assay of peroxidase activity[P].US6432662B1.