养阴益气、布津通络法对干燥综合征小鼠干预作用及机理研究
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摘要
干燥综合征(Sjogren's syndrome,SS)是一种侵犯外分泌腺体尤以唾液腺和泪腺为主的慢性自身免疫性疾病。导师汪悦教授根据长期的临床经验,运用养阴益气、布津通络法治疗SS,方用增液布津汤在临床取得良好效果,本文通过实验研究进一步探讨养阴益气、布津通络法治疗SS的作用机制,为临床工作提供充实的依据。
目的:
探讨中医学对原发性干燥综合征发病机理的认识,从基础研究角度探讨养阴益气、布津通络法对干燥综合征小鼠干预作用及免疫功能影响,并从组织和细胞学角度探讨增液布津汤对干燥综合征小鼠AQP5的表达影响,为阐明运用养阴益气、布津通络法治疗原发性干燥综合征小鼠的作用机制提供依据,从而在证实其确切疗效的同时明确其作用部位,为更好地运用于临床提供理论与实验依据。
方法:
1.理论研究方法:检索古今中外文献对干燥综合征认识,并进行分析比较。
2.实验研究方法:实验研究部分用BALB/c小鼠颌下腺免疫造成SS小鼠模型,同时给予增液布津汤。在8周的实验中,每周测定唾液流量,每4天测定体重、摄食量、饮水量。取胸腺和颌下腺计算胸腺指数和颌下腺指数,并观察颌下腺病理变化。并用流式细胞仪测定外周血CD4+/CD8+,ELIST法测定血清IFN-γ、IL-1、IL-6含量,计算IFN-γ/IL-6。用免疫组化法检测小鼠颌下腺AQP5的分布表达变化。
结果:
1.理论研究结果:在文献资料分析的基础上,结合导师汪悦教授的临床经验,认为:由于燥热之邪导致阴津耗伤,津能载气,气随津耗,则气阴两虚是大多数pSS病人的共同转归,因此确立养阴益气、布津通络为治疗大法,根据气能生津、气能行津的原则,重用养阴益气药,运用增液布津汤治疗pSS,取得良好的效果。
2.实验研究结果:
2.1在症状表现上,与正常小鼠相比,模型小鼠唾液流量减少、体重减轻、饮水量增加。而增液布津汤大、中、小剂量组均能不同程度改善小鼠口渴症状。
2.2在免疫功能方面,与正常小鼠相比,模型组颌下腺指数降低、胸腺指数增加,T、B淋巴细胞转化增殖能力比值增加、颌下腺出现淋巴细胞浸润等病理变化,CD4+/CD8+增加,而给予增液布津汤能改善这些症状并减轻病理改变。
2.3细胞因子IFN-γ、IL-1、IL-6变化情况。结果显示:与模型组比较,细胞因子IFN-γ、IL-1、IL-6在增液布津汤作用下,IL-1、IFN-γ与模型组比较显著降低,其中小剂量组降低明显P＜0.01。给予药物后IL-6也有不同程度降低,各组间IL-6无显著性差异。
2.4对AQP5的影响。我们又研究养阴益气、布津通络对小鼠颌下腺细胞AQP5的影响。通过比较研究,显示:增液布津汤能改善小鼠颌下腺细胞AQP5的表达,与国内外学者研究有类似之处,但也有相异之处。
结论:
1.增液布津汤具有抑制小鼠腺体淋巴细胞浸润、抑制T、B淋巴细胞增殖、改善CD4~+/CD8~+比值,能够纠正免疫紊乱。
2.增液布津汤可能通过抑制细胞因子IL-6、IFN-γ蛋白合成及分泌,间接地抑制了Th细胞的分化与增殖,从而缓解SS小鼠颌下腺淋巴细胞浸润,通过调节Th1/Th2细胞因子失衡来保护SS小鼠,进一步说明细胞因子在SS唾液分泌过程中发挥重要作用。
3.增液布津汤能改变小鼠颌下腺细胞AQP5分布表达,与唾液分泌功能有关,因此为增液布津汤治疗SS提供了依据。
创新点:
首次从气阴两虚的角度,研究养阴益气、布津通络法对SS小鼠干预作用及其机理,得出该治法通过调节T淋巴细胞亚群、Th1/Th2细胞因子失衡,改变颌下腺细胞AQP5分布表达,最终得到改善唾液分泌功能、减轻干燥症状的目的。进一步说明养阴益气、布津通络法对SS作用机制是多途径、多环节、多靶点的。
Sjogren's syndrome (SS) is a chronic autoimmune disease that invades the exocrine glands, especially salivary and lachrymal glands. According to Professor Wangyue's long-term clinical experience, prescription of Zengye Bujin Decoction, which has obtained good results in clinic, was used to treat pSS by nourishing yin and invigorating qi as well as distributing fluid and freeing collaterals. This essay further studies the mechanism of Chinese medical therapy on pSS by experiments, which provides powerful evidences for clinical applications.
Objectives:
To explore the pathological mechanism of SS in TCM, effects of Chinese medical therapy on the interference and immune function in SS-mice from basic researches, influence of Zengye Bujin Tang on AQP5 expression in SS-mice based on histology and cytology. Therefore it could provide evidence for the explanation of the mechanism of Chinese medical therapy in SS-mice, the curative effects as well as the site of action and serves as the theoretical and experimental evidences for clinical applications.
Method:
1. Method of theoretical research: By retrieving literature at home and abroad, from ancient to present, information related to SS is analyzed and compared.
2. Method of experimental research: we made mice-models of SS induced bysub maxillary gland immune in BALB/C mice, and then fed Zengye Bujin Tang to them. During the eight weeks, salivary flow was measured every week, weight, food and water intakes every four days. By removing Thymus Index and Submandibular Gland Index, They were counted and pathological changes of sub maxillary gland were observed. We still tested CD4f/CD8f in the peripheral blood by flow cytometry and the serum content of IFN-γ, IL-1 and IL-6 through ELIST, calculated IFN-γand IL-6,detected the AQP5 expression changes of the sub maxillary gland cells in mice through immunohistochemicalstaining.
Results:
1. Results of theoretical research: Based on the analysis of literature, combined with professor Wangyue's long-term experience, a conclusion was drew. Due to body fluid carrying qi and loss of body fluid followed by exhaustion of qi, dryness-heat leads to loss of yin-fluid so that the developments were both deficiency of yin and qi for most SS patients. Therefore the therapy of nourishing yin for invigorating qi and distributing fluid for freeing collaterals was established according to mutual-action of qi and body fluid. Drugs for nourishing yin and invigorating qi were greatly used in Zengye Bujin Decoction to treat pSS and good curative effects have been obtained.
2. Experimental research results:
2.1 Symptomatic manifestations Compared with the normal mice, model mice were with the signs of saliva flow reduction, weight loses and water intake. While the high, middle and low dosage groups of Zengye Bujin Decoction relieved the mice's thirsty in various degrees.
2.2 Immunological functions The model mice had some pathological changes, such as decreased sub-mandibular gland, and thymus index increased and proliferation ratio of T,B lymphocyte, lymphocytic infiltration of submandibular gland, and CD4+/CD8+ also increased. Zengye Bujin Decoction could improve these symptoms and relieve the pathological changes.
2.3 Changes of cytokine(IFN-γ, IL-1, IL-6) The resuits showed that under the effect of Zengye Bujin Decoction IFN-γin serum cytokine(IFN-γ, IL-1, IL-6) were much lower than the model groups, among which low dosage group reduced significantly(p<0.01). After taking drugs, IL-6 also reduced in various degrees, but there was no significant difference among the groups.
2.4 Influences on AQP5 Influences on aquaporin5(AQP5) of the mouse submandibular gland cells by nourishing yin and invigorating qi as well as distributing water and free collaterals were studied in the paper. Comparative studies revealed that Zengye Bujin Decoction could improve the expressions of AQP5 of the mouse submandibular gland cells, revealing similarities and differences with research done at home and abroad.
Conclusion:
1. Zengye bujin Decoction could inhibit the lymphocytic infiltration of submandibular gland in mouse, restrain the proliferation of T and B lymphocyte, and improve the ratio of CD4+/CD8+ and balance immune disorders.
2. Zengye Bujin Decoction would inhibit the differentiation and proliferation of Th cells indirectly to relieve the lymphocytic infiltration on submandibular gland of SS mouse by means of inhibiting protein synthesis and secretion of cytokine(IL-6, IFN-γ). Through balanced Th1/Th2 cytokine to protect SS mice, the method deeply illustrated that cytokines played an important role in the process of saliva excretion on SS.
3. Zengye Bujin Decoction could change the distribution and expression of AQP5 of the mouse submandibular gland cells which is related to saliva secretion, therefore it provided evidences for treating SS with Zengye Bujin Decoction.
Creative points:
According to yin and qi deficiency, we explored into the effect and mechanism of interventing SS mouse by the therapy of nourishing yin for invigorating qi and distributing fluid for freeing collaterals for the first time. The research showed that this therapy could change the distribution and expression of AQP5 of the mouse submandibular gland to improve the function of salivary secretion by regulating T-lymphocyte subsets and the imbalance of Th1/Th2 cytokine. It further illustrated that the therapy of nourishing yin for invigorating qi and distributing fluid for freeing collaterals was multi-channel, multi-link and multi-target.
目的:
探讨中医学对原发性干燥综合征发病机理的认识,从基础研究角度探讨养阴益气、布津通络法对干燥综合征小鼠干预作用及免疫功能影响,并从组织和细胞学角度探讨增液布津汤对干燥综合征小鼠AQP5的表达影响,为阐明运用养阴益气、布津通络法治疗原发性干燥综合征小鼠的作用机制提供依据,从而在证实其确切疗效的同时明确其作用部位,为更好地运用于临床提供理论与实验依据。
方法:
1.理论研究方法:检索古今中外文献对干燥综合征认识,并进行分析比较。
2.实验研究方法:实验研究部分用BALB/c小鼠颌下腺免疫造成SS小鼠模型,同时给予增液布津汤。在8周的实验中,每周测定唾液流量,每4天测定体重、摄食量、饮水量。取胸腺和颌下腺计算胸腺指数和颌下腺指数,并观察颌下腺病理变化。并用流式细胞仪测定外周血CD4+/CD8+,ELIST法测定血清IFN-γ、IL-1、IL-6含量,计算IFN-γ/IL-6。用免疫组化法检测小鼠颌下腺AQP5的分布表达变化。
结果:
1.理论研究结果:在文献资料分析的基础上,结合导师汪悦教授的临床经验,认为:由于燥热之邪导致阴津耗伤,津能载气,气随津耗,则气阴两虚是大多数pSS病人的共同转归,因此确立养阴益气、布津通络为治疗大法,根据气能生津、气能行津的原则,重用养阴益气药,运用增液布津汤治疗pSS,取得良好的效果。
2.实验研究结果:
2.1在症状表现上,与正常小鼠相比,模型小鼠唾液流量减少、体重减轻、饮水量增加。而增液布津汤大、中、小剂量组均能不同程度改善小鼠口渴症状。
2.2在免疫功能方面,与正常小鼠相比,模型组颌下腺指数降低、胸腺指数增加,T、B淋巴细胞转化增殖能力比值增加、颌下腺出现淋巴细胞浸润等病理变化,CD4+/CD8+增加,而给予增液布津汤能改善这些症状并减轻病理改变。
2.3细胞因子IFN-γ、IL-1、IL-6变化情况。结果显示:与模型组比较,细胞因子IFN-γ、IL-1、IL-6在增液布津汤作用下,IL-1、IFN-γ与模型组比较显著降低,其中小剂量组降低明显P＜0.01。给予药物后IL-6也有不同程度降低,各组间IL-6无显著性差异。
2.4对AQP5的影响。我们又研究养阴益气、布津通络对小鼠颌下腺细胞AQP5的影响。通过比较研究,显示:增液布津汤能改善小鼠颌下腺细胞AQP5的表达,与国内外学者研究有类似之处,但也有相异之处。
结论:
1.增液布津汤具有抑制小鼠腺体淋巴细胞浸润、抑制T、B淋巴细胞增殖、改善CD4~+/CD8~+比值,能够纠正免疫紊乱。
2.增液布津汤可能通过抑制细胞因子IL-6、IFN-γ蛋白合成及分泌,间接地抑制了Th细胞的分化与增殖,从而缓解SS小鼠颌下腺淋巴细胞浸润,通过调节Th1/Th2细胞因子失衡来保护SS小鼠,进一步说明细胞因子在SS唾液分泌过程中发挥重要作用。
3.增液布津汤能改变小鼠颌下腺细胞AQP5分布表达,与唾液分泌功能有关,因此为增液布津汤治疗SS提供了依据。
创新点:
首次从气阴两虚的角度,研究养阴益气、布津通络法对SS小鼠干预作用及其机理,得出该治法通过调节T淋巴细胞亚群、Th1/Th2细胞因子失衡,改变颌下腺细胞AQP5分布表达,最终得到改善唾液分泌功能、减轻干燥症状的目的。进一步说明养阴益气、布津通络法对SS作用机制是多途径、多环节、多靶点的。
Sjogren's syndrome (SS) is a chronic autoimmune disease that invades the exocrine glands, especially salivary and lachrymal glands. According to Professor Wangyue's long-term clinical experience, prescription of Zengye Bujin Decoction, which has obtained good results in clinic, was used to treat pSS by nourishing yin and invigorating qi as well as distributing fluid and freeing collaterals. This essay further studies the mechanism of Chinese medical therapy on pSS by experiments, which provides powerful evidences for clinical applications.
Objectives:
To explore the pathological mechanism of SS in TCM, effects of Chinese medical therapy on the interference and immune function in SS-mice from basic researches, influence of Zengye Bujin Tang on AQP5 expression in SS-mice based on histology and cytology. Therefore it could provide evidence for the explanation of the mechanism of Chinese medical therapy in SS-mice, the curative effects as well as the site of action and serves as the theoretical and experimental evidences for clinical applications.
Method:
1. Method of theoretical research: By retrieving literature at home and abroad, from ancient to present, information related to SS is analyzed and compared.
2. Method of experimental research: we made mice-models of SS induced bysub maxillary gland immune in BALB/C mice, and then fed Zengye Bujin Tang to them. During the eight weeks, salivary flow was measured every week, weight, food and water intakes every four days. By removing Thymus Index and Submandibular Gland Index, They were counted and pathological changes of sub maxillary gland were observed. We still tested CD4f/CD8f in the peripheral blood by flow cytometry and the serum content of IFN-γ, IL-1 and IL-6 through ELIST, calculated IFN-γand IL-6,detected the AQP5 expression changes of the sub maxillary gland cells in mice through immunohistochemicalstaining.
Results:
1. Results of theoretical research: Based on the analysis of literature, combined with professor Wangyue's long-term experience, a conclusion was drew. Due to body fluid carrying qi and loss of body fluid followed by exhaustion of qi, dryness-heat leads to loss of yin-fluid so that the developments were both deficiency of yin and qi for most SS patients. Therefore the therapy of nourishing yin for invigorating qi and distributing fluid for freeing collaterals was established according to mutual-action of qi and body fluid. Drugs for nourishing yin and invigorating qi were greatly used in Zengye Bujin Decoction to treat pSS and good curative effects have been obtained.
2. Experimental research results:
2.1 Symptomatic manifestations Compared with the normal mice, model mice were with the signs of saliva flow reduction, weight loses and water intake. While the high, middle and low dosage groups of Zengye Bujin Decoction relieved the mice's thirsty in various degrees.
2.2 Immunological functions The model mice had some pathological changes, such as decreased sub-mandibular gland, and thymus index increased and proliferation ratio of T,B lymphocyte, lymphocytic infiltration of submandibular gland, and CD4+/CD8+ also increased. Zengye Bujin Decoction could improve these symptoms and relieve the pathological changes.
2.3 Changes of cytokine(IFN-γ, IL-1, IL-6) The resuits showed that under the effect of Zengye Bujin Decoction IFN-γin serum cytokine(IFN-γ, IL-1, IL-6) were much lower than the model groups, among which low dosage group reduced significantly(p<0.01). After taking drugs, IL-6 also reduced in various degrees, but there was no significant difference among the groups.
2.4 Influences on AQP5 Influences on aquaporin5(AQP5) of the mouse submandibular gland cells by nourishing yin and invigorating qi as well as distributing water and free collaterals were studied in the paper. Comparative studies revealed that Zengye Bujin Decoction could improve the expressions of AQP5 of the mouse submandibular gland cells, revealing similarities and differences with research done at home and abroad.
Conclusion:
1. Zengye bujin Decoction could inhibit the lymphocytic infiltration of submandibular gland in mouse, restrain the proliferation of T and B lymphocyte, and improve the ratio of CD4+/CD8+ and balance immune disorders.
2. Zengye Bujin Decoction would inhibit the differentiation and proliferation of Th cells indirectly to relieve the lymphocytic infiltration on submandibular gland of SS mouse by means of inhibiting protein synthesis and secretion of cytokine(IL-6, IFN-γ). Through balanced Th1/Th2 cytokine to protect SS mice, the method deeply illustrated that cytokines played an important role in the process of saliva excretion on SS.
3. Zengye Bujin Decoction could change the distribution and expression of AQP5 of the mouse submandibular gland cells which is related to saliva secretion, therefore it provided evidences for treating SS with Zengye Bujin Decoction.
Creative points:
According to yin and qi deficiency, we explored into the effect and mechanism of interventing SS mouse by the therapy of nourishing yin for invigorating qi and distributing fluid for freeing collaterals for the first time. The research showed that this therapy could change the distribution and expression of AQP5 of the mouse submandibular gland to improve the function of salivary secretion by regulating T-lymphocyte subsets and the imbalance of Th1/Th2 cytokine. It further illustrated that the therapy of nourishing yin for invigorating qi and distributing fluid for freeing collaterals was multi-channel, multi-link and multi-target.
引文
[1]Fox RI,Stern M,Michelson P.Update in Sj(O|¨)gren's syndrome.Curr Opin Rheumatol 2000,12:391-396
[2]Bowman SJ,Ibrahim GH,Holmes G et al.Estimating the prevalence among Caucasian women of primary Sj(O|¨)gren's syndrome in two general practices in Birmingham,UK.Scand J Rheumatol 2004,33:39-43
[3]Pillemer SR,Matteson EL,Jacobsson LT et al.Incidence of physician-diagnosed primary Sj(O|¨)gren's syndrome in residents of Olmsted County,Minnesota.Mayo Clin Proc 2001,76:593-599
[4】郭强,顾越英.干燥综合征发病机制研究进展.《上海免疫学杂志》2002年第22卷第3期:215-217
[5]Jamadi M,Balla S,Dalaan A,et al.Cytokine profile in systemic lupus erythematosus,rheumatoid arthritis,and other rheumatic diseases.Jclin Immunol,1993,13:58
[6]Price EJ,Venables PJ.The etiopathogenesis of Sj(O|¨)gren's syndrome.Semin Arthritis Rheum1995,25:117-133
[7]Fox RI,Saito I.Sj(O|¨)gren's syndrome:immunologic and neuroendocrine mechanisms.Adv Exp Med Biol 1994,350:609-621
[8]Mossman TR,Cherwinski HM,Bond MW et al.Two types of murine helper T cell clone,I:definition according to profiles of lymphokine activities and secreted proteins.J Immunol 1986,136:2348-2357
[9]Romagnani S.Lymphokine production by human T cells in disease states.Annu Rev Immunol 1994,12:227-257
[10]Fox RI,Kang HI,Ando D et al.Cytokine mRNA expression in salivary gland biopsies of Sj(O|¨)gren's syndrome.J Immunol 1994,152:5532-5539
[11]Ohyama Y,Nakamura S,Matsuzaki G et al.Cytokine messenger RNA expression in the labial salivary glands of patients with Sj(O|¨)gren's syndrome.Arthritis Rheum 1996,39:1376-1384
[12]Rowe D,Griffiths M,Stewart J et al.HLA class Ⅰ and Ⅱ,interferon,interleukin 2,and the interleukin 2 receptor expression on labial biopsy specimens from patients with Sj(O|¨)gren's syndrome.Ann Rheum Dis 1987,46:580-586
[13]MITSIASDI,TZIOUFASAG,VEIOPOULOUC,et al.The Th1、Th2 cytokine balance changes with the progress of the immunopathological lesion of Sj(O|¨)gren's syndrome.Clin Explmmunol,2002,128:562-568
[14]EdgardoC,Kolkowski,PeterReth,et al.Th1 predominance and Perforin Expression in minor Salivary Glands from Patients with Primary Sj(O|¨)gren's syndrome.Journal of Autoimmunity,1999,13:155-162
[15]Moore M,Ma T,Yang B,et al.Tear secretion by lacrimal glands in transgenic mice lacking water channels AQP1,AQP3,AQP4 and AQP5[J].Eye Res,2002,70(5):557-562
[16]Ma T,Song Y,Gillespie A,et al.Defective secretion of saliva in transgenic mice lacking aquaporin-5 water channels[J].J Biol Chem,1999,274(29):20071-20074
[17]Gresz V,Kwon T H,Hurley P T,et al.Identification and localization of aquaporin water channels in human salivary glands[J].Am J Physiol Gastrointest Liver Physiol,2001,281(1):G247-254
[18]Steinfeld SD,Appelboom T,Delporte C.Treatment with infliximab restores normal aquaporins distribution in minor salivary glands of patients with Sj(O|¨)gren's syndrome[J].Arthritis Rheum,2002,46:2249-2251
[19]Steinfeld SD,Demols P,Salmon I,et al.Infliximab in patients with primary Sj(O|¨)gren's syndrome:a pilot study[J].Arthritis Rheum,2001,44:2371-2375
[20]FoxR I.Sj(O|¨)gren's syndrome Controversies and progress[J].Clin Lab Med,1977,17(3):431-444
[21]Baudouin C,Pisella P J,Brignole F.Current treatments of xerophthalmia in Sj(O|¨)gren's syndrome.Rev Med Interne 2004,25:376-382
[22]Fox RI.Sj(O|¨)gren's syndrome evolving therapies.Expert Opin Investig Drugs.2003,12:247-254.
[23]Cassolato SF,Turnbull RS.Xerostomia:clinical aspects and treatment.Gerodontology 2003,20:64-77
[24]Porter SR,Scully C,Hegarty AM.An update of the etiology and management of xerostomia.Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2004,97:28-46
[25】金桂兰,汪悦,等.增液润燥汤治疗干燥综合征47例临床研究.南京中医药大学学报[J].2008,4:268-269
[1]傅志宜.临床皮肤病鉴别诊断学[M].北京:中国医学院科学出版社,1990.642
[2]路志正,焦树德,等.痹病论治学[M].北京:人民卫生出版社,1989:281
[3]张立亭,傅新利,等.张鸣鹤辨冶干燥综合征经验[J].山东中医药大学学报,2000,24(2):120-121
[4】路志正.路志正医林集腋[M].北京:人民卫生出版社,1990:154-155
[5]秦长林.从干燥综合征看“燥必入血”的病变特点[J].山东中医杂志,2000,19(2):710
[6]方朝晖,孔梅.干燥综合片治验[J].安徽中医临床杂志,1998.10(4):228
[7]吴坚,朱良春.朱良春治疗干燥综合征经验[J].实用中医杂志,2006,22(8):501
[8]母小真.陈湘君治疗干燥综合征的经验[J].辽宁中医杂志.2002.29(1):11
[9]董振华.活血化瘀法在干燥综合征中的运用[J].北京中医,2001.20(3):9-11
[10]顾勤,刘菊妍.周仲英教授治疗干燥综合征经验介绍[J].新中医,2002,34(9):7-8
[11]黄萌高.干燥综合征的证治体会[J].上海中医药杂志,2006.40(9)
[12】胡建东,薛鸾,施晓芬等.解毒清络生津方治疗原发性干燥综合征33例[J].湖南中医杂志,2005,2(14):51-52
[13]曾庆祥.路志正治疗干燥综合征经验[J].中医杂志,2004,45(6):413-415
[14]王燕青,刘学法,李达祥.从瘀论治干燥综合征探微[J].中医函授通讯,1998;17(4):16-17
[15]马武开.干燥综合征的中医病因病机讨论[J].中医药研究,2000;8(4):2
[16]张淑英.清热除湿法治疗干燥综合征18例[J】.中医杂志,2001;42(8):506
[17】钱垠,金实.从肺论治干燥综合征[J].南京中医药大学学报(自然科学版),2002;18(5):268-269
[18]戴恩来,王庆胜.从肺论治干燥综合征体会[J].甘肃中医学院学报,2005,22(2):9-10
[19]李晓梅,欧阳兵.干燥综合征从脾阴虚论治刍议[J].山东中医药大学学报,2003,27(6)
[20】李荣峰.东垣清燥汤为主治疗干燥综合征[J】.中医药学刊,2006,24(1):162-163
[21】潘利,杨德才.从气论治干燥综合征的思路探析[J].湖北中医杂志,2005,27(10):15-16
[22】姚楚芳,蒋树龙.中医异病同治法临床应用体会[J】.上海中医药大学学报,2004,18(3):26-27
[23】吕文增.三仁汤治疗原发性干燥综合征80例[J].辽宁中医杂志,2004,3(110):862
[24】姜迎萍,李靖.干燥综合征的中医证治规律探讨[J].四川中医,2003;4(21):7
[25]马武开.干燥综合征辨证分型探讨[J】.光明中医,2001,16(92):30-32
[26】傅宗翰,刘永年.干燥综合征的辨证施治规律[J】.南京中医学院学报,1987;11(3):1122
[27】郝伟欣,董振华.干燥综合征106例中医证候分类回顾性研究[J】.中医杂志,2006,47(7):528-530
[28]吴玲燕.宋欣伟教授辨治干燥综合征新思路[J].中华中医药学刊,2007;25(8):1564-1565
[29】叶海军.从三焦论治干燥综合征[J].辽宁中医杂志,2004,31(6):477
[30]陈湘君,陈浩.酸甘化阴法治疗干燥综合征[J].上海中医药大学学报,2005,19(3):22-23
[31】周翠英,潘文萍,王燕.燥毒清饮治疗干燥综合征临床研究[J].山东中医杂志,2005,24(12):715-716
[32】蒋明.风湿病学[M】.北京:北京科技出版社,1995,2048
[33]姜迎萍,刘浩.干燥综合征组方用药规律浅识[J].Chinese Archives of Traditional Chinese Medicine,2002,20(5):654
[34]潘新.干燥综合征的中医诊治体会[J].湖北中医学院学报,2007.9(1):61-62
[35】苑丽娟.三段三方治疗干燥综合征[J].辽宁中医杂志,1996,23(8):353-353
[36]谷家立.干燥综合征的五脏证刍议[J].中医杂志.2000,41(10):635-638
[37]姜黎平.玉女甘露汤治疗干燥综合征[J].浙江中医学院学报,1990,(3):21
[38]刘永年.中国当代名医名方录[M】.北京,中国大百科全书出版社,2000:402
[39】杜秀兰,宋绍亮,等.益气养阴润燥汤治疗干燥综合征10例[J].山东中医杂志,1994,13(12):535
[40】王慕虹,张新,等.益气生津汤治疗干燥综合征的体会[J].实用中西医结合杂志,1997,10(5):471
[41]顾廷全.玉液汤加味治疗干燥综合征26例[J].实用中医药杂志,1994,10(4):15
[42]王心光.养阴清肺汤合清开灵注射液治疗干燥综合征20例[J].吉林中医药,2000,20(1):34
[43】廖承建.门冬清肺饮加减治疗干燥综合征32例[J].新中医,1999,31(4):44
[44]徐宜厚.针药并治干燥综合征11例[J].中医杂志,1990,31(8):42
[45]赵振兴.加味引火汤治疗干燥综合征15例临床观察[J].河北中医,1998,20(4):226
[46]刘薛乡.润燥六黄汤合雷公藤制剂治疗干燥综合征20例[J].山东中医杂志,1993,(2):13
[47】周小平.桂附地黄丸加减治疗干燥综合征15例[J].湖南中医杂志,1998,14(4):39
[48]陈一峰,任军生,等.清燥救肺汤合大黄蟅虫丸治疗干燥综合征26例-附西药治疗12例对照观察[J].浙江中医杂志,2000,35(2):57
[49】李新一.大黄蠊虫丸治疗干燥综合征35例观察[J].黑龙江中医药,2001,(6):13
[50】王慧,刘维,等.润燥合剂治疗干燥综合征40例疗效观察[J].天津中医学院学报,2000,19(2):26
[51】杨少锋.继发性口眼干燥综合征34例疗效分析[J].中国中西医结合杂志 1993,(4):230
[52】蒋熙.四妙勇安汤加味治疗风湿类疾病举隅[J].江苏中医,1999,20(2):19
[53】赵祖友.复元活血汤临床应用[J].陕西中医,1995,16(9):417
[54】黄德友,马荣华.真武汤新用2则[J].国医论坛,1995,10(2):14
[55】邓裔超,邓淑云.邓启源老中医运用拯阴理劳汤撷菁[J].福建中医药,2000,31(6):18
[56]马伟明,杜玉琳.补阳还武汤的临床运用[J].浙江中医学院学报,1997,21(6):32
[57]王耕.小柴胡汤加减治疗干燥综合征[J].浙江中医杂志,1987,(8):364
[58】肖宝兰,黄志英,等.生脉散加味治疗原发性干燥综合征1例[J].广东医学,2000,21(8):638
[59]邱建荣.浅谈干燥综合征的治疗[J].浙江中医学院学报,1996,20(6):6
[60]周尔文,韩露霞,等.猪苓汤治疗继发性口眼干燥综合征的体会与理论探讨[J].中国医药学报,1994,9(6):30
[61]Yue Wang,Tingting Yan,Jian Shen,etc.Preventive effect of Ophiopogon japonicus polysaccharides on an autoallergic mouse model for Sj(O|¨)gren's syndrome by regulating the Th1/Th2 cytokine imbalance.Journal of Ethnopharmacology 114(2007)246-253.
[62】周定华,张前德,等.丹地琼玉颗粒对干燥综合征小鼠唾液腺的影响[J].南京医科大学学报(自然科学版),2005,25(4):266-272
[63】俞创奇,邱蔚六等.干燥综合征动物模型的建立及中药疗效的观察[J].上海口腔医学,1999,8(1)24-26
[64】杨敬宁,周彬.沙参麦冬汤对阴虚大鼠免疫功能的影响[J].实用中医药志,2005,12,12(21):715-716
[65]刘英,周翠英.清燥布津法对干燥综合征小鼠下颌腺AQP5表达的影晌[J].山东中医药大学2005届博士学位论文摘要
[66]王睿.生津口服液对SS模型鼠治疗机理的初步研究.[D].北京:北京中医药大学,2004.
[67】孙宇扬,张丽.生津口服液对干燥综合征动物模型治疗作用的初步研究.中药药理与临床,2006:2(1):51-53.
[68】吴晓丹,孙丽英,等.增液汤对干燥综合征小颌下腺Th1样细胞因子IL-2、IFN-R及Th2样细胞因子IL-4、IL-6影响[J].中医药信息2008;25(3):34-36
[69]张乃峥.原发性干燥综合征的诊断[J].中华风湿病学杂志,2004,8(4):193-195
[70]张乃峥,施胜全,要庆平.原发性干燥综合征的流行病学调查[J].中华内科杂志,1993.32(8)522-524
[71]周建耀,刘佳等.干燥综合征血清睾酮测定的临床意义[J].实用医技杂志,2007,14(8)3931-3932.
[72]Sullivan DA,Sato EH.potential therapeutic approach for the hormonal treatment of lacrinal glandl dysfunction in Sj(O|¨)gren's spndrome[J].clin Immunol Immunopathoi,1992,64:916.
[73]Shumin Wen.Association ofEpstein-Barr Virus(EBV) with Sj(O|¨)gren's syndrome.Differential EBV Expression between Epitheial Cells and Lymphocytes in Salivaty Glands.J Pathol,1996,149:1511
[74]Fox RI,Theofilopoulos AN.Sj(O|¨)gren's syndrome:pathogensis and prospects for therapy.Exp Opin Invest Drugs,1996,5:1
[75】孙小平.舍古林综合征中医药治疗前后T细胞群的变化[J].现代口腔医学杂志.1994,(1):15-17
[76】汪迅等.干燥综合征患者外周血中T淋巴细胞亚群的分析与临床意义[J].中国药物与临 床.2007,7(4):268-270
[77]Ruddy S,Harris ED Jr,Sledye CB.Kelley's textbook of rheummatology[M].6th ed.Sj(O|¨)gren's syndrome[J].Br J Rheumatol,1998,37:779
[78]Cuello C,Palladinetti P,Tedla N,et al.Chemokine expression and leu-cocyte infilteation 1993.32(8)522-524
[79]Anaya JM,Talal N.Sj(O|¨)gren's syndrome comes of age[J]Semin Arthritis Rheum,1999,28:355
[80]Sargusa K,Ishimaru N,Yanagi K,et al.Treatment with anti-CD86costimulatory molecule prevents the autoimmune lesions in murine Sj(O|¨)gren's syndrome through up-regulated Th2response[J].Gin ExpI mmunol,2000,119:354
[81]Robinson CP,Brayer J,Ysmachika S,et al.Transfer of human serum IgG to nonobese diabetic Igμnull mice reveals a role for autoantibodies in the loss of secretory function of exocrine tissues in Sj(O|¨)gren's syndrome[J],proc Natl Acad Sci USA,1998,95:7538
[82]Stott DI,Hiepe F,Hummel Metal.Antigeirdriven clonal proliferation of B cells within the target tissue of an autoimmune disease ahe salivary glands of patients with Sj(O|¨)gren's syndrome[J].J Gin Invest,1998,102:938
[83]吴晓梅,黄雨梅,王兰兰,刘瑾.干燥综合征患者的泪腺及血浆中的白介素2、白介素6及其受体的研究[J].华西医大学报,JWCUNMS 2000;31(4):543-544
[84]Fox RI,Bumol T,Fantozzi R,et al.Expression of histocompatibility antigen HLA-DR by Salivary gland epithelial cells in Sj(O|¨)gren's syndrome.Arhritis Rheum,1986,29(9):1105
[85]Rose NR,Mackay IR.The autoimmune diseases[M].3rd ed.San Diego:Academic Press,1998:381
[86]杨军,肖林,等.AQP-5在干燥综合征小鼠颌下腺中的表达.2004.26(2):121-124
[87]Fox RI.Sj(O|¨)gren's syndrome.Lancet,2005,366:321-331
[88]Ma T,Song Y,Gillespie A,et al.Defective secretion of Saliva in transgenic mice lacking aquaporin-5 water channels.J Biol Chem,1999,274:20071-20074
[89]Konttinen YT,Tensing EK,Laine M,et al.Abnormal distribution of aquaporin-5 in Salivary glands in the NOD mouse model for Sj(O|¨)gren's syndrome.J Rheumatol,2005,32:1071-1075
[90]MA T,Fukuda N,Song Y,et al.Lung fluid transport in aquaporin-5 knockout mice.J Clin invest,2000,105:93-100
[91]Song Y,Fukuda N,Bai C,et al.Role of aquaporins in alveolar fluid clearance in neonatal and adult lung,and in oedema formation following acute lung injury:studies in transgenic aquaporin null mice.J Physiol,2000,525:771-779
[92]Krane CM,Fortner CN,Hand AR,et al.aquaporin-5 deficient mouse lungs are hyperresponsive to cholinergic stimulation.Proc Natl Acad Sci USA,2001,98:14114-14119
[93]Rabinowitz YS,Dong L,Wistow G..Gene expression profile studies of human keratoconus cornea for neibank:a novel cornea-ex-pressed gene and the absence of transcripts for aquaporin-5.invest Ophthalmol Vis Sci,2005,46:1239-1246
[94】杨军,肖林,等.AQP-5在干燥综合征小鼠颌下腺中的表达[J].第三军医大学学报 2004,26(2):122-124
[95]李菁,赵岩,唐福林,杜德顺.水分子通道蛋白—5在干燥综合征唇腺中的异常表达[J].中华风湿病学杂志,2005.,9(8):453-456
[96]Yang F,Kawedia J,Menon AG.Cyclic AMP regulates aquaporen-5 expression both transeriqtional and post-transcriptional leves through a protein kinase A pathway.J Biol Chem,2003,278:32173-32180
[97]Towne JE,Krane CM,Bachurski CJ,et al.Tumor necrosis factor-α inhibits aquaporin-5expression in mouse lung epithelial cells.J Biol Chem,2001,276:18657-18664
[98]Sankar V,Brennan MT,Kok MR,et al.Etanercept in Sj(O|¨)gren's syndrome:atwelve-week randomixed,double-blind,placebocontrolled pilot clinical trial.Arthritis Rheum,2004,50:2240-2245
[99]Smith KJ,Sliddiqui AA,Midica LA,et al.interferon-alpha upregulates gene expression of aquaporen-5 in human parotid glands.J Interferon Cytokine Res,1999,19:929-935
[100]Cummins MJ,Papas A,Kammer GM,et al.Treatment of primary Sj(O|¨)gren's syndrome with low-dose human interferon alfa adminis tered by the oromucosal route:combined phase Ⅲresult.Arthritis Rheum 2003,49:585-593
[101]Yamamoto H,Sims NE,Macauley SP,et al.Alterations in the secretory response of NOD mice to muscarinic receptor stimulation.Clin Immunol Immunopathol.1996,78:245-255
[102]赖蓓,陈巧林,张翠华,王振刚,栗占国.免疫印迹法检测抗胆碱能毒蕈碱受体3抗体及其在干燥综合征诊断中的意义[J].中华风湿病学杂志,2005,9(7):409-413
[103]Waterman SA,Gordon TP,Rischmueller M.Inhibitory effects of muscarinic recepor autoantibodies on parasympathetic neurotrans-mission in Sj(O|¨)gren's syndrome.Arthritis Rheum,2000,43:1647-1654
[104]Cuello C,Palladinetti P,Tedla N,et al.Chemokine expression and leu-cocyte infilteation Sj(O|¨)gren's syndrome[J].Br J Rheumatol,1998,37:779
[105]Rose NR,Mackay IR.The autoimmune diseases[M].3rd ed.San Diego:Academic Press,1998:381
[106]Fox RI,Dixon R,Guarrasi V,et al.Treatment of primary Sj(O|¨)gren's syndrome with hydrochloroquine:a retrospective,open-label sutdy.Lupus,1996,51:531-536
[107]Mauoussakis MN,Moutsopoulos HM.Antimalarials in Sj(O|¨)gren's syndrome the Greek experience.Lupus,1996,5:528-530.
[108]FoxRI,Stem M,ichelson P.Update Sj(O|¨)gren's syndrome[J].eurr Rheumatol,2000,12(5):391
[109]Yamano S,Atkinson JC,Baum BJ,et al.Salivary gland cytokine gene expression in NOD aud BALB/c mice.Autoimmunity,1999,92:265-275.
[110]Yanagi K,Ishimaru N,Haneji N,et al.Anti-120 000 alpha-fodrin immune response with Th1cytokine profile in the NOD mouse model of Sj(O|¨)gren's syndrome.Eur J Immounol,1998, 28:3336-3345
[111]Dean DH, Hiramoto RN J Oral Pathol, 1984, 13(1):63-68
[112]Yoshiyo DH, Mitsunobu S, Katsuiku H. Induction of experimental allergic sialodenitis in mice. Am J Pathol, 1985, 118:476-483
[113]Mya Mu, Dipshikha C, Kazuko T, et al.Production of experimental autoimmune sialodenitis in mice immunized with homolmgous Salivary gland extract and klebsiella 03 lipopolysaccharide. J Autoimmunity2001,16:29-36.
[114]Suzuki K, Makino M, Okada Y,et al. Exocrinopathy resembling SjOgren's syndrome induced by a murine retrovirus.Lab Invest, 1993,69:430-435
[115]Yoshiyo H, Yoshiaki Y, Hideo Y, et al.Development of allergic sialodenitis in mice immunized with mumps virus-infected submandibular Salivary gland. Am J Pathol, 1986, 123:271-279
[1]Lowry OH,Rosebrough NJ,Farr AL,Randall RJ.Protein measurement with the Folin phenol reagent.J Biol Chem 1951;193:265-275
[2]Groom J,Kalled SL,Cutler AH,Olson C,Woodcock SA,Schneider P et al.Association of BAFF/BLyS overexpression and altered B cell differentiation with Sjogren's syndrome.J Clin Invest 2002;109:59-68
[3]Chisholm DM,Mason DK.Salivary gland function in Sj(O|¨)gren's syndrome:a review[J].Br dent Dent J,1973,135(9):393
[4]方福德,周吕,丁濂.现代医学实验技巧全书(下册)[M].北京医科大学、中国协和医科大学联合出版社,1995,316
[5]肖林,胡静,等.水通道蛋白在干燥综合征患者唇腺中表达的实验研究.临床口腔医学杂志2004,20:457
[1】姜迎萍,李靖.干燥综合征的中医证治规律探讨[J].四川中医,2003;4(21):7
[2】钟起诚.干燥综合征之中医病因病机探讨[J].中国中医药信息杂志,2003,10:73
[3】潘文奎.口眼干燥综合征的辨证论治[J】.辽宁中医杂志,1988,12:21
[4]马永桢.运用滋阴法治疗干燥综合征[J】.甘肃中医,1995,8:13
[5】董振华.活血化瘀法在干燥综合征中的应用[J】.北京中医,2001,3:9
[6】洪庆祥.12例干燥综合征临床观察[J].上海中医药杂志,1995,9:16
[7】马永桢.运用滋阴法治疗干燥综合征[J].甘肃中医,1995,8:13-4
[8]王鹏宇,王静.辨证治疗干燥综合征73例[J].浙江中医杂志,197,32:164-165
[9】张立亭.张鸣鹤辨治干燥综合征经验[J].山东中医药大学学报,2000,4:20-21
[10]孙素平,潘文萍,周翠英.试论干燥综合征以燥毒为本[J].山东中医杂志 2001,20:581-582
[11】董振华.干燥综合征的中医治疗[J】.中国医刊,1999,35:47
[12】徐治鸿,孙小平,赵芳.活血生津药治疗SS对红细胞变形性的影响[J].中华口腔医学杂志,1992;27:162
[13】王燕青,刘学法.从瘀论治干燥综合征探微[J].中医函授通讯,1998,17:16
[14】方朝辉,孔梅.干燥综合征治验[J].安徽中医临床杂志 1998,10:228
[15]徐治鸿,孙小平,赵芳等.活血生津药治疗干燥综合征对红细胞变形性的影响[J】.中华口腔医学杂志,1992,27(3):162
[16]周世文,徐传福.多糖的免疫药理作用[J].中国生化药物杂志,1994,15:143
[17】曹广文,杜平.黄芪多糖、刺五加多糖和枸杞多糖在体内对LAK细胞抗肿瘤活性的调节作用[J].第二军医大学学报,1993,14:10
[18]Tvede N,Heilmam C,Christensen LD.Interleukin 2 receptor expression by human blood lymphocytes after vaccination with pneumococcal polysaccharides.Clin Exp Immunol,1989,76:404
[19]余伯阳,殷霞,张春红等.麦冬多糖的免疫活性研究[J】.中国药科大学学报,1991,22:286-288
[20]Liu J,Chen T,Yu B et al.Ruscogenin glycoside(Lm-3) isolated from Liriope muscari inhibits lymphocyte adhesion to extracellular matrix.J Pharm Pharmacol 2002,54:959-965
[21]Wu F,Cao J,Jiang J et al.Ruscogenin glycoside(Lm-3) isolated from Liriope muscari improves liver injury by- dysfunctioning liver-infiltrating lymphocytes.J Pharm Pharmacol 2001;53:681-688
[22]余伯阳,殷霞等.短草山麦冬皂甙C的药理活性研究[J].中国药科大学学报,1994,25:286-288
[23】黄厚才.麦冬对大鼠血小板聚集率的影响[J】.上海实验动物科学,2001,3:167-168
[24】沈玉先,魏伟,徐叔云等.白芍总苷的药理作用及机制[M].第二军医大学出版社,1997,162-167
[25】王兴旺,陈敏珠,徐叔云等.白芍总苷对T淋巴细胞亚群的作用[J】.中国药理学通报,1992,8:340-344
[26]王斌,陈敏珠,徐叔云.白芍总苷对大鼠腹腔巨噬细胞产生肿瘤坏死因子的调节机制[J】.中国药理学通报,1997,3:255-257
[27]郭浩,魏伟,陈敏珠等.白芍总苷对T细胞调节功能的影响[J】.中国药理学与毒理学杂志,1993,3:193-196
[28】厉小梅,李向培.白芍总苷治疗干燥综合征的有效性和安全性评价[J】.安徽医学,2006,27:370-371
[29】张秋海,欧兴长.桃仁的研究进展[J】.实用中西医结合杂志,1993,6(3):163
[30】朱萱萱,朱芳,施荣山等.桃仁、防已提取物对大鼠血小板聚集作用的研究[J].中医药研究,2000,16(3):44-45
[31】刘英,张伟刚,王雅贤等.炒桃仁总蛋白对小鼠B细胞功能影响的实验研究[J】.中医药学报,2001,29(2):55-56
[32】沈丕安.现代中医免疫病学[M].北京:人民卫生出版社,2003:85-148
[33】周敏,李秉琦,黄雨梅等.干燥综合征的血液流变学变化[J】.华西口腔医学杂志,1994,12:272-273
[34】董振华,郝炜新,刘晋河等.60例干燥综合征患者血液流变学检测及养阴生津中药治疗效果观察[J】.中国中西医结合杂志 1998;18:155
[35】杜秀兰,宋绍亮,尹玉茹.益气养阴润燥汤治疗干燥征10例[J】.山东中医杂志,1994;13(12):535
[36]钟琴,严鲁萍,曹永芬等.六味地黄丸加味治疗干燥综合征48例临床观察[J].贵阳中医学院学报,2002,4(3):34
[37】胡北平,丁永敏,张琴韵.舍占林氏综合征中西医结合诊断本征及治疗的探讨[J].口腔医学,1989,9(1):1-3
[38】黄剑英,周文豪,楼雁飞.治疗更年期口咽干燥综合征30例体会[J】.浙江中医学院学报,2000,24(3):34
[39】申康.六味地黄丸汤合增液汤治疗原发性干燥综合征30例[J】.山东中医杂志,2002,21(8):467-469
[40]施荣山,朱萱萱,邱召娟.津血源胶囊治疗干燥综合征的实验研究[J].中药药理与临床,2002,14(5):26-27
[41]Fox RI,Kang HI,Ando D et al.Cytokine mRNA expression in salivary gland biopsies of Sjogren's syndrome.J Immunol 1994,152:5532-5539
[42]Ohyama Y,Nakamura S,Matsuzaki G et al.Cytokine messenger RNA expression in the labial salivary glands of patients with Sjogren's syndrome.Arthritis Rheum 1996,39:1376-84
[43]Takahashi M,Mimura Y,Hayashi Y.Role of the ICAM-1/LFA-1 pathway during the development of autoimmune dacryoadenitis in an animal model for Sjogren's syndrome.Pathobiology 1996,64:269-274
[44]Fox RI,Kang Ho-Il,Ando D et al.Cytokine mRNA expression in salivary gland biopsies of Sjogren's syndrome.J Immunol 1994,152:5532-5540
[45]Baudouin C,De Saint Jean M,Brignole F et al.Expression of apoptotic and inflammatory markers in the human conjunctival epithelium.Invest Ophthalmol Vis Sci 1998,39:S551
[46]Baudouin C,Brignole F,Becquet F et al.Flow cytometry in impression cytology specimens:a new method for evaluation of conjunctival inflammation.Invest Ophthalmol Vis Sci 1997,38:1458-1464
[47]Brignole F,De Saint Jean M,Becquet F et al.Expression of Fas-Fas ligand antigens and apoptotic marker APO2.7 by the human conjunctival epithelium:positive correlation with class Ⅱ HLA-DR expression in inflammatory conditions.Exp Eye Res 1998;67:687-697
[48]肖林,胡静,等.水通道蛋白在干燥综合征患者唇腺中表达的实验研究[J】.临床口腔医学杂志 2004,20:457
[49]Price EJ,Venables PJ.The etiopathogenesis of Sjogren's syndrome.Semin Arthritis Rheum 1995;25:117-133
[50]Fox RI,Saito I.Sjogren's syndrome:immunologic and neuroendocrine mechanisms.Adv Exp Med Biol 1994,350:609-621
[51]Boehm U,Klamp T,Groot Met al.Cellular responses to interferon-gamma.Annu Rev Immunol 1997,15:749-795
[52]Mossman TR,Cherwinski HM,Bond MW et al.Two types of murine helper T cell clone,Ⅰ:definition according to profiles of lymphokine activities and secreted proteins.J Immunol 1986,136:2348-2357
[53]Romagnani S.Lymphokine production by human T cells in disease states.Annu Rev Immunol 1994,12:227-257
[54】吴翰桂,骆大法.IL-6负调控TH1细胞分化的分子机制.台州学院学报,2002,24(6):68
[55]Smith J K,Siddiqui AA,Modica L A,et al.Interferon-alpha upregulates gene expression of aquaporin-5 in human parotid glands[J].J Interferon Cytokine Res,1999,19(8):929-935
[1]Chisholm DM,Mason DK.Labial Salivary gland biopsy Sj(O|¨)gren's syndrome.J Clin Pathol,1996,76:427-430
[2]Greenspan JS,Daniels TE,Talal N.The histopatology of Sj(O|¨)gren's syndrome in labial Salivary gland biopsies.Oral Surg,1974,37:217
[3]Daniels TE,Whitcher JP.Association of patterns of labial Salivary gland in flammation with keratconjunctivitis sicca.Arthritis Rheum,1994,37:869
[4】陈慧颖,文竹咸,马正中.唇腺的年龄性改变及其在Sjogren综合征诊断中的意义[J].中国医学科学院学报,1993,15(5):343-348
[5】董怡.2002年修订的干燥综合征国际分类(诊断)标准[J].中华风湿病学杂志 2002;6:257
[6】李瑞琳,张凤山.第四届全国中西医结合风湿病学术会议纪要[J].中国中西结合杂志2001;21:157-159
[2]Bowman SJ,Ibrahim GH,Holmes G et al.Estimating the prevalence among Caucasian women of primary Sj(O|¨)gren's syndrome in two general practices in Birmingham,UK.Scand J Rheumatol 2004,33:39-43
[3]Pillemer SR,Matteson EL,Jacobsson LT et al.Incidence of physician-diagnosed primary Sj(O|¨)gren's syndrome in residents of Olmsted County,Minnesota.Mayo Clin Proc 2001,76:593-599
[4】郭强,顾越英.干燥综合征发病机制研究进展.《上海免疫学杂志》2002年第22卷第3期:215-217
[5]Jamadi M,Balla S,Dalaan A,et al.Cytokine profile in systemic lupus erythematosus,rheumatoid arthritis,and other rheumatic diseases.Jclin Immunol,1993,13:58
[6]Price EJ,Venables PJ.The etiopathogenesis of Sj(O|¨)gren's syndrome.Semin Arthritis Rheum1995,25:117-133
[7]Fox RI,Saito I.Sj(O|¨)gren's syndrome:immunologic and neuroendocrine mechanisms.Adv Exp Med Biol 1994,350:609-621
[8]Mossman TR,Cherwinski HM,Bond MW et al.Two types of murine helper T cell clone,I:definition according to profiles of lymphokine activities and secreted proteins.J Immunol 1986,136:2348-2357
[9]Romagnani S.Lymphokine production by human T cells in disease states.Annu Rev Immunol 1994,12:227-257
[10]Fox RI,Kang HI,Ando D et al.Cytokine mRNA expression in salivary gland biopsies of Sj(O|¨)gren's syndrome.J Immunol 1994,152:5532-5539
[11]Ohyama Y,Nakamura S,Matsuzaki G et al.Cytokine messenger RNA expression in the labial salivary glands of patients with Sj(O|¨)gren's syndrome.Arthritis Rheum 1996,39:1376-1384
[12]Rowe D,Griffiths M,Stewart J et al.HLA class Ⅰ and Ⅱ,interferon,interleukin 2,and the interleukin 2 receptor expression on labial biopsy specimens from patients with Sj(O|¨)gren's syndrome.Ann Rheum Dis 1987,46:580-586
[13]MITSIASDI,TZIOUFASAG,VEIOPOULOUC,et al.The Th1、Th2 cytokine balance changes with the progress of the immunopathological lesion of Sj(O|¨)gren's syndrome.Clin Explmmunol,2002,128:562-568
[14]EdgardoC,Kolkowski,PeterReth,et al.Th1 predominance and Perforin Expression in minor Salivary Glands from Patients with Primary Sj(O|¨)gren's syndrome.Journal of Autoimmunity,1999,13:155-162
[15]Moore M,Ma T,Yang B,et al.Tear secretion by lacrimal glands in transgenic mice lacking water channels AQP1,AQP3,AQP4 and AQP5[J].Eye Res,2002,70(5):557-562
[16]Ma T,Song Y,Gillespie A,et al.Defective secretion of saliva in transgenic mice lacking aquaporin-5 water channels[J].J Biol Chem,1999,274(29):20071-20074
[17]Gresz V,Kwon T H,Hurley P T,et al.Identification and localization of aquaporin water channels in human salivary glands[J].Am J Physiol Gastrointest Liver Physiol,2001,281(1):G247-254
[18]Steinfeld SD,Appelboom T,Delporte C.Treatment with infliximab restores normal aquaporins distribution in minor salivary glands of patients with Sj(O|¨)gren's syndrome[J].Arthritis Rheum,2002,46:2249-2251
[19]Steinfeld SD,Demols P,Salmon I,et al.Infliximab in patients with primary Sj(O|¨)gren's syndrome:a pilot study[J].Arthritis Rheum,2001,44:2371-2375
[20]FoxR I.Sj(O|¨)gren's syndrome Controversies and progress[J].Clin Lab Med,1977,17(3):431-444
[21]Baudouin C,Pisella P J,Brignole F.Current treatments of xerophthalmia in Sj(O|¨)gren's syndrome.Rev Med Interne 2004,25:376-382
[22]Fox RI.Sj(O|¨)gren's syndrome evolving therapies.Expert Opin Investig Drugs.2003,12:247-254.
[23]Cassolato SF,Turnbull RS.Xerostomia:clinical aspects and treatment.Gerodontology 2003,20:64-77
[24]Porter SR,Scully C,Hegarty AM.An update of the etiology and management of xerostomia.Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2004,97:28-46
[25】金桂兰,汪悦,等.增液润燥汤治疗干燥综合征47例临床研究.南京中医药大学学报[J].2008,4:268-269
[1]傅志宜.临床皮肤病鉴别诊断学[M].北京:中国医学院科学出版社,1990.642
[2]路志正,焦树德,等.痹病论治学[M].北京:人民卫生出版社,1989:281
[3]张立亭,傅新利,等.张鸣鹤辨冶干燥综合征经验[J].山东中医药大学学报,2000,24(2):120-121
[4】路志正.路志正医林集腋[M].北京:人民卫生出版社,1990:154-155
[5]秦长林.从干燥综合征看“燥必入血”的病变特点[J].山东中医杂志,2000,19(2):710
[6]方朝晖,孔梅.干燥综合片治验[J].安徽中医临床杂志,1998.10(4):228
[7]吴坚,朱良春.朱良春治疗干燥综合征经验[J].实用中医杂志,2006,22(8):501
[8]母小真.陈湘君治疗干燥综合征的经验[J].辽宁中医杂志.2002.29(1):11
[9]董振华.活血化瘀法在干燥综合征中的运用[J].北京中医,2001.20(3):9-11
[10]顾勤,刘菊妍.周仲英教授治疗干燥综合征经验介绍[J].新中医,2002,34(9):7-8
[11]黄萌高.干燥综合征的证治体会[J].上海中医药杂志,2006.40(9)
[12】胡建东,薛鸾,施晓芬等.解毒清络生津方治疗原发性干燥综合征33例[J].湖南中医杂志,2005,2(14):51-52
[13]曾庆祥.路志正治疗干燥综合征经验[J].中医杂志,2004,45(6):413-415
[14]王燕青,刘学法,李达祥.从瘀论治干燥综合征探微[J].中医函授通讯,1998;17(4):16-17
[15]马武开.干燥综合征的中医病因病机讨论[J].中医药研究,2000;8(4):2
[16]张淑英.清热除湿法治疗干燥综合征18例[J】.中医杂志,2001;42(8):506
[17】钱垠,金实.从肺论治干燥综合征[J].南京中医药大学学报(自然科学版),2002;18(5):268-269
[18]戴恩来,王庆胜.从肺论治干燥综合征体会[J].甘肃中医学院学报,2005,22(2):9-10
[19]李晓梅,欧阳兵.干燥综合征从脾阴虚论治刍议[J].山东中医药大学学报,2003,27(6)
[20】李荣峰.东垣清燥汤为主治疗干燥综合征[J】.中医药学刊,2006,24(1):162-163
[21】潘利,杨德才.从气论治干燥综合征的思路探析[J].湖北中医杂志,2005,27(10):15-16
[22】姚楚芳,蒋树龙.中医异病同治法临床应用体会[J】.上海中医药大学学报,2004,18(3):26-27
[23】吕文增.三仁汤治疗原发性干燥综合征80例[J].辽宁中医杂志,2004,3(110):862
[24】姜迎萍,李靖.干燥综合征的中医证治规律探讨[J].四川中医,2003;4(21):7
[25]马武开.干燥综合征辨证分型探讨[J】.光明中医,2001,16(92):30-32
[26】傅宗翰,刘永年.干燥综合征的辨证施治规律[J】.南京中医学院学报,1987;11(3):1122
[27】郝伟欣,董振华.干燥综合征106例中医证候分类回顾性研究[J】.中医杂志,2006,47(7):528-530
[28]吴玲燕.宋欣伟教授辨治干燥综合征新思路[J].中华中医药学刊,2007;25(8):1564-1565
[29】叶海军.从三焦论治干燥综合征[J].辽宁中医杂志,2004,31(6):477
[30]陈湘君,陈浩.酸甘化阴法治疗干燥综合征[J].上海中医药大学学报,2005,19(3):22-23
[31】周翠英,潘文萍,王燕.燥毒清饮治疗干燥综合征临床研究[J].山东中医杂志,2005,24(12):715-716
[32】蒋明.风湿病学[M】.北京:北京科技出版社,1995,2048
[33]姜迎萍,刘浩.干燥综合征组方用药规律浅识[J].Chinese Archives of Traditional Chinese Medicine,2002,20(5):654
[34]潘新.干燥综合征的中医诊治体会[J].湖北中医学院学报,2007.9(1):61-62
[35】苑丽娟.三段三方治疗干燥综合征[J].辽宁中医杂志,1996,23(8):353-353
[36]谷家立.干燥综合征的五脏证刍议[J].中医杂志.2000,41(10):635-638
[37]姜黎平.玉女甘露汤治疗干燥综合征[J].浙江中医学院学报,1990,(3):21
[38]刘永年.中国当代名医名方录[M】.北京,中国大百科全书出版社,2000:402
[39】杜秀兰,宋绍亮,等.益气养阴润燥汤治疗干燥综合征10例[J].山东中医杂志,1994,13(12):535
[40】王慕虹,张新,等.益气生津汤治疗干燥综合征的体会[J].实用中西医结合杂志,1997,10(5):471
[41]顾廷全.玉液汤加味治疗干燥综合征26例[J].实用中医药杂志,1994,10(4):15
[42]王心光.养阴清肺汤合清开灵注射液治疗干燥综合征20例[J].吉林中医药,2000,20(1):34
[43】廖承建.门冬清肺饮加减治疗干燥综合征32例[J].新中医,1999,31(4):44
[44]徐宜厚.针药并治干燥综合征11例[J].中医杂志,1990,31(8):42
[45]赵振兴.加味引火汤治疗干燥综合征15例临床观察[J].河北中医,1998,20(4):226
[46]刘薛乡.润燥六黄汤合雷公藤制剂治疗干燥综合征20例[J].山东中医杂志,1993,(2):13
[47】周小平.桂附地黄丸加减治疗干燥综合征15例[J].湖南中医杂志,1998,14(4):39
[48]陈一峰,任军生,等.清燥救肺汤合大黄蟅虫丸治疗干燥综合征26例-附西药治疗12例对照观察[J].浙江中医杂志,2000,35(2):57
[49】李新一.大黄蠊虫丸治疗干燥综合征35例观察[J].黑龙江中医药,2001,(6):13
[50】王慧,刘维,等.润燥合剂治疗干燥综合征40例疗效观察[J].天津中医学院学报,2000,19(2):26
[51】杨少锋.继发性口眼干燥综合征34例疗效分析[J].中国中西医结合杂志 1993,(4):230
[52】蒋熙.四妙勇安汤加味治疗风湿类疾病举隅[J].江苏中医,1999,20(2):19
[53】赵祖友.复元活血汤临床应用[J].陕西中医,1995,16(9):417
[54】黄德友,马荣华.真武汤新用2则[J].国医论坛,1995,10(2):14
[55】邓裔超,邓淑云.邓启源老中医运用拯阴理劳汤撷菁[J].福建中医药,2000,31(6):18
[56]马伟明,杜玉琳.补阳还武汤的临床运用[J].浙江中医学院学报,1997,21(6):32
[57]王耕.小柴胡汤加减治疗干燥综合征[J].浙江中医杂志,1987,(8):364
[58】肖宝兰,黄志英,等.生脉散加味治疗原发性干燥综合征1例[J].广东医学,2000,21(8):638
[59]邱建荣.浅谈干燥综合征的治疗[J].浙江中医学院学报,1996,20(6):6
[60]周尔文,韩露霞,等.猪苓汤治疗继发性口眼干燥综合征的体会与理论探讨[J].中国医药学报,1994,9(6):30
[61]Yue Wang,Tingting Yan,Jian Shen,etc.Preventive effect of Ophiopogon japonicus polysaccharides on an autoallergic mouse model for Sj(O|¨)gren's syndrome by regulating the Th1/Th2 cytokine imbalance.Journal of Ethnopharmacology 114(2007)246-253.
[62】周定华,张前德,等.丹地琼玉颗粒对干燥综合征小鼠唾液腺的影响[J].南京医科大学学报(自然科学版),2005,25(4):266-272
[63】俞创奇,邱蔚六等.干燥综合征动物模型的建立及中药疗效的观察[J].上海口腔医学,1999,8(1)24-26
[64】杨敬宁,周彬.沙参麦冬汤对阴虚大鼠免疫功能的影响[J].实用中医药志,2005,12,12(21):715-716
[65]刘英,周翠英.清燥布津法对干燥综合征小鼠下颌腺AQP5表达的影晌[J].山东中医药大学2005届博士学位论文摘要
[66]王睿.生津口服液对SS模型鼠治疗机理的初步研究.[D].北京:北京中医药大学,2004.
[67】孙宇扬,张丽.生津口服液对干燥综合征动物模型治疗作用的初步研究.中药药理与临床,2006:2(1):51-53.
[68】吴晓丹,孙丽英,等.增液汤对干燥综合征小颌下腺Th1样细胞因子IL-2、IFN-R及Th2样细胞因子IL-4、IL-6影响[J].中医药信息2008;25(3):34-36
[69]张乃峥.原发性干燥综合征的诊断[J].中华风湿病学杂志,2004,8(4):193-195
[70]张乃峥,施胜全,要庆平.原发性干燥综合征的流行病学调查[J].中华内科杂志,1993.32(8)522-524
[71]周建耀,刘佳等.干燥综合征血清睾酮测定的临床意义[J].实用医技杂志,2007,14(8)3931-3932.
[72]Sullivan DA,Sato EH.potential therapeutic approach for the hormonal treatment of lacrinal glandl dysfunction in Sj(O|¨)gren's spndrome[J].clin Immunol Immunopathoi,1992,64:916.
[73]Shumin Wen.Association ofEpstein-Barr Virus(EBV) with Sj(O|¨)gren's syndrome.Differential EBV Expression between Epitheial Cells and Lymphocytes in Salivaty Glands.J Pathol,1996,149:1511
[74]Fox RI,Theofilopoulos AN.Sj(O|¨)gren's syndrome:pathogensis and prospects for therapy.Exp Opin Invest Drugs,1996,5:1
[75】孙小平.舍古林综合征中医药治疗前后T细胞群的变化[J].现代口腔医学杂志.1994,(1):15-17
[76】汪迅等.干燥综合征患者外周血中T淋巴细胞亚群的分析与临床意义[J].中国药物与临 床.2007,7(4):268-270
[77]Ruddy S,Harris ED Jr,Sledye CB.Kelley's textbook of rheummatology[M].6th ed.Sj(O|¨)gren's syndrome[J].Br J Rheumatol,1998,37:779
[78]Cuello C,Palladinetti P,Tedla N,et al.Chemokine expression and leu-cocyte infilteation 1993.32(8)522-524
[79]Anaya JM,Talal N.Sj(O|¨)gren's syndrome comes of age[J]Semin Arthritis Rheum,1999,28:355
[80]Sargusa K,Ishimaru N,Yanagi K,et al.Treatment with anti-CD86costimulatory molecule prevents the autoimmune lesions in murine Sj(O|¨)gren's syndrome through up-regulated Th2response[J].Gin ExpI mmunol,2000,119:354
[81]Robinson CP,Brayer J,Ysmachika S,et al.Transfer of human serum IgG to nonobese diabetic Igμnull mice reveals a role for autoantibodies in the loss of secretory function of exocrine tissues in Sj(O|¨)gren's syndrome[J],proc Natl Acad Sci USA,1998,95:7538
[82]Stott DI,Hiepe F,Hummel Metal.Antigeirdriven clonal proliferation of B cells within the target tissue of an autoimmune disease ahe salivary glands of patients with Sj(O|¨)gren's syndrome[J].J Gin Invest,1998,102:938
[83]吴晓梅,黄雨梅,王兰兰,刘瑾.干燥综合征患者的泪腺及血浆中的白介素2、白介素6及其受体的研究[J].华西医大学报,JWCUNMS 2000;31(4):543-544
[84]Fox RI,Bumol T,Fantozzi R,et al.Expression of histocompatibility antigen HLA-DR by Salivary gland epithelial cells in Sj(O|¨)gren's syndrome.Arhritis Rheum,1986,29(9):1105
[85]Rose NR,Mackay IR.The autoimmune diseases[M].3rd ed.San Diego:Academic Press,1998:381
[86]杨军,肖林,等.AQP-5在干燥综合征小鼠颌下腺中的表达.2004.26(2):121-124
[87]Fox RI.Sj(O|¨)gren's syndrome.Lancet,2005,366:321-331
[88]Ma T,Song Y,Gillespie A,et al.Defective secretion of Saliva in transgenic mice lacking aquaporin-5 water channels.J Biol Chem,1999,274:20071-20074
[89]Konttinen YT,Tensing EK,Laine M,et al.Abnormal distribution of aquaporin-5 in Salivary glands in the NOD mouse model for Sj(O|¨)gren's syndrome.J Rheumatol,2005,32:1071-1075
[90]MA T,Fukuda N,Song Y,et al.Lung fluid transport in aquaporin-5 knockout mice.J Clin invest,2000,105:93-100
[91]Song Y,Fukuda N,Bai C,et al.Role of aquaporins in alveolar fluid clearance in neonatal and adult lung,and in oedema formation following acute lung injury:studies in transgenic aquaporin null mice.J Physiol,2000,525:771-779
[92]Krane CM,Fortner CN,Hand AR,et al.aquaporin-5 deficient mouse lungs are hyperresponsive to cholinergic stimulation.Proc Natl Acad Sci USA,2001,98:14114-14119
[93]Rabinowitz YS,Dong L,Wistow G..Gene expression profile studies of human keratoconus cornea for neibank:a novel cornea-ex-pressed gene and the absence of transcripts for aquaporin-5.invest Ophthalmol Vis Sci,2005,46:1239-1246
[94】杨军,肖林,等.AQP-5在干燥综合征小鼠颌下腺中的表达[J].第三军医大学学报 2004,26(2):122-124
[95]李菁,赵岩,唐福林,杜德顺.水分子通道蛋白—5在干燥综合征唇腺中的异常表达[J].中华风湿病学杂志,2005.,9(8):453-456
[96]Yang F,Kawedia J,Menon AG.Cyclic AMP regulates aquaporen-5 expression both transeriqtional and post-transcriptional leves through a protein kinase A pathway.J Biol Chem,2003,278:32173-32180
[97]Towne JE,Krane CM,Bachurski CJ,et al.Tumor necrosis factor-α inhibits aquaporin-5expression in mouse lung epithelial cells.J Biol Chem,2001,276:18657-18664
[98]Sankar V,Brennan MT,Kok MR,et al.Etanercept in Sj(O|¨)gren's syndrome:atwelve-week randomixed,double-blind,placebocontrolled pilot clinical trial.Arthritis Rheum,2004,50:2240-2245
[99]Smith KJ,Sliddiqui AA,Midica LA,et al.interferon-alpha upregulates gene expression of aquaporen-5 in human parotid glands.J Interferon Cytokine Res,1999,19:929-935
[100]Cummins MJ,Papas A,Kammer GM,et al.Treatment of primary Sj(O|¨)gren's syndrome with low-dose human interferon alfa adminis tered by the oromucosal route:combined phase Ⅲresult.Arthritis Rheum 2003,49:585-593
[101]Yamamoto H,Sims NE,Macauley SP,et al.Alterations in the secretory response of NOD mice to muscarinic receptor stimulation.Clin Immunol Immunopathol.1996,78:245-255
[102]赖蓓,陈巧林,张翠华,王振刚,栗占国.免疫印迹法检测抗胆碱能毒蕈碱受体3抗体及其在干燥综合征诊断中的意义[J].中华风湿病学杂志,2005,9(7):409-413
[103]Waterman SA,Gordon TP,Rischmueller M.Inhibitory effects of muscarinic recepor autoantibodies on parasympathetic neurotrans-mission in Sj(O|¨)gren's syndrome.Arthritis Rheum,2000,43:1647-1654
[104]Cuello C,Palladinetti P,Tedla N,et al.Chemokine expression and leu-cocyte infilteation Sj(O|¨)gren's syndrome[J].Br J Rheumatol,1998,37:779
[105]Rose NR,Mackay IR.The autoimmune diseases[M].3rd ed.San Diego:Academic Press,1998:381
[106]Fox RI,Dixon R,Guarrasi V,et al.Treatment of primary Sj(O|¨)gren's syndrome with hydrochloroquine:a retrospective,open-label sutdy.Lupus,1996,51:531-536
[107]Mauoussakis MN,Moutsopoulos HM.Antimalarials in Sj(O|¨)gren's syndrome the Greek experience.Lupus,1996,5:528-530.
[108]FoxRI,Stem M,ichelson P.Update Sj(O|¨)gren's syndrome[J].eurr Rheumatol,2000,12(5):391
[109]Yamano S,Atkinson JC,Baum BJ,et al.Salivary gland cytokine gene expression in NOD aud BALB/c mice.Autoimmunity,1999,92:265-275.
[110]Yanagi K,Ishimaru N,Haneji N,et al.Anti-120 000 alpha-fodrin immune response with Th1cytokine profile in the NOD mouse model of Sj(O|¨)gren's syndrome.Eur J Immounol,1998, 28:3336-3345
[111]Dean DH, Hiramoto RN J Oral Pathol, 1984, 13(1):63-68
[112]Yoshiyo DH, Mitsunobu S, Katsuiku H. Induction of experimental allergic sialodenitis in mice. Am J Pathol, 1985, 118:476-483
[113]Mya Mu, Dipshikha C, Kazuko T, et al.Production of experimental autoimmune sialodenitis in mice immunized with homolmgous Salivary gland extract and klebsiella 03 lipopolysaccharide. J Autoimmunity2001,16:29-36.
[114]Suzuki K, Makino M, Okada Y,et al. Exocrinopathy resembling SjOgren's syndrome induced by a murine retrovirus.Lab Invest, 1993,69:430-435
[115]Yoshiyo H, Yoshiaki Y, Hideo Y, et al.Development of allergic sialodenitis in mice immunized with mumps virus-infected submandibular Salivary gland. Am J Pathol, 1986, 123:271-279
[1]Lowry OH,Rosebrough NJ,Farr AL,Randall RJ.Protein measurement with the Folin phenol reagent.J Biol Chem 1951;193:265-275
[2]Groom J,Kalled SL,Cutler AH,Olson C,Woodcock SA,Schneider P et al.Association of BAFF/BLyS overexpression and altered B cell differentiation with Sjogren's syndrome.J Clin Invest 2002;109:59-68
[3]Chisholm DM,Mason DK.Salivary gland function in Sj(O|¨)gren's syndrome:a review[J].Br dent Dent J,1973,135(9):393
[4]方福德,周吕,丁濂.现代医学实验技巧全书(下册)[M].北京医科大学、中国协和医科大学联合出版社,1995,316
[5]肖林,胡静,等.水通道蛋白在干燥综合征患者唇腺中表达的实验研究.临床口腔医学杂志2004,20:457
[1】姜迎萍,李靖.干燥综合征的中医证治规律探讨[J].四川中医,2003;4(21):7
[2】钟起诚.干燥综合征之中医病因病机探讨[J].中国中医药信息杂志,2003,10:73
[3】潘文奎.口眼干燥综合征的辨证论治[J】.辽宁中医杂志,1988,12:21
[4]马永桢.运用滋阴法治疗干燥综合征[J】.甘肃中医,1995,8:13
[5】董振华.活血化瘀法在干燥综合征中的应用[J】.北京中医,2001,3:9
[6】洪庆祥.12例干燥综合征临床观察[J].上海中医药杂志,1995,9:16
[7】马永桢.运用滋阴法治疗干燥综合征[J].甘肃中医,1995,8:13-4
[8]王鹏宇,王静.辨证治疗干燥综合征73例[J].浙江中医杂志,197,32:164-165
[9】张立亭.张鸣鹤辨治干燥综合征经验[J].山东中医药大学学报,2000,4:20-21
[10]孙素平,潘文萍,周翠英.试论干燥综合征以燥毒为本[J].山东中医杂志 2001,20:581-582
[11】董振华.干燥综合征的中医治疗[J】.中国医刊,1999,35:47
[12】徐治鸿,孙小平,赵芳.活血生津药治疗SS对红细胞变形性的影响[J].中华口腔医学杂志,1992;27:162
[13】王燕青,刘学法.从瘀论治干燥综合征探微[J].中医函授通讯,1998,17:16
[14】方朝辉,孔梅.干燥综合征治验[J].安徽中医临床杂志 1998,10:228
[15]徐治鸿,孙小平,赵芳等.活血生津药治疗干燥综合征对红细胞变形性的影响[J】.中华口腔医学杂志,1992,27(3):162
[16]周世文,徐传福.多糖的免疫药理作用[J].中国生化药物杂志,1994,15:143
[17】曹广文,杜平.黄芪多糖、刺五加多糖和枸杞多糖在体内对LAK细胞抗肿瘤活性的调节作用[J].第二军医大学学报,1993,14:10
[18]Tvede N,Heilmam C,Christensen LD.Interleukin 2 receptor expression by human blood lymphocytes after vaccination with pneumococcal polysaccharides.Clin Exp Immunol,1989,76:404
[19]余伯阳,殷霞,张春红等.麦冬多糖的免疫活性研究[J】.中国药科大学学报,1991,22:286-288
[20]Liu J,Chen T,Yu B et al.Ruscogenin glycoside(Lm-3) isolated from Liriope muscari inhibits lymphocyte adhesion to extracellular matrix.J Pharm Pharmacol 2002,54:959-965
[21]Wu F,Cao J,Jiang J et al.Ruscogenin glycoside(Lm-3) isolated from Liriope muscari improves liver injury by- dysfunctioning liver-infiltrating lymphocytes.J Pharm Pharmacol 2001;53:681-688
[22]余伯阳,殷霞等.短草山麦冬皂甙C的药理活性研究[J].中国药科大学学报,1994,25:286-288
[23】黄厚才.麦冬对大鼠血小板聚集率的影响[J】.上海实验动物科学,2001,3:167-168
[24】沈玉先,魏伟,徐叔云等.白芍总苷的药理作用及机制[M].第二军医大学出版社,1997,162-167
[25】王兴旺,陈敏珠,徐叔云等.白芍总苷对T淋巴细胞亚群的作用[J】.中国药理学通报,1992,8:340-344
[26]王斌,陈敏珠,徐叔云.白芍总苷对大鼠腹腔巨噬细胞产生肿瘤坏死因子的调节机制[J】.中国药理学通报,1997,3:255-257
[27]郭浩,魏伟,陈敏珠等.白芍总苷对T细胞调节功能的影响[J】.中国药理学与毒理学杂志,1993,3:193-196
[28】厉小梅,李向培.白芍总苷治疗干燥综合征的有效性和安全性评价[J】.安徽医学,2006,27:370-371
[29】张秋海,欧兴长.桃仁的研究进展[J】.实用中西医结合杂志,1993,6(3):163
[30】朱萱萱,朱芳,施荣山等.桃仁、防已提取物对大鼠血小板聚集作用的研究[J].中医药研究,2000,16(3):44-45
[31】刘英,张伟刚,王雅贤等.炒桃仁总蛋白对小鼠B细胞功能影响的实验研究[J】.中医药学报,2001,29(2):55-56
[32】沈丕安.现代中医免疫病学[M].北京:人民卫生出版社,2003:85-148
[33】周敏,李秉琦,黄雨梅等.干燥综合征的血液流变学变化[J】.华西口腔医学杂志,1994,12:272-273
[34】董振华,郝炜新,刘晋河等.60例干燥综合征患者血液流变学检测及养阴生津中药治疗效果观察[J】.中国中西医结合杂志 1998;18:155
[35】杜秀兰,宋绍亮,尹玉茹.益气养阴润燥汤治疗干燥征10例[J】.山东中医杂志,1994;13(12):535
[36]钟琴,严鲁萍,曹永芬等.六味地黄丸加味治疗干燥综合征48例临床观察[J].贵阳中医学院学报,2002,4(3):34
[37】胡北平,丁永敏,张琴韵.舍占林氏综合征中西医结合诊断本征及治疗的探讨[J].口腔医学,1989,9(1):1-3
[38】黄剑英,周文豪,楼雁飞.治疗更年期口咽干燥综合征30例体会[J】.浙江中医学院学报,2000,24(3):34
[39】申康.六味地黄丸汤合增液汤治疗原发性干燥综合征30例[J】.山东中医杂志,2002,21(8):467-469
[40]施荣山,朱萱萱,邱召娟.津血源胶囊治疗干燥综合征的实验研究[J].中药药理与临床,2002,14(5):26-27
[41]Fox RI,Kang HI,Ando D et al.Cytokine mRNA expression in salivary gland biopsies of Sjogren's syndrome.J Immunol 1994,152:5532-5539
[42]Ohyama Y,Nakamura S,Matsuzaki G et al.Cytokine messenger RNA expression in the labial salivary glands of patients with Sjogren's syndrome.Arthritis Rheum 1996,39:1376-84
[43]Takahashi M,Mimura Y,Hayashi Y.Role of the ICAM-1/LFA-1 pathway during the development of autoimmune dacryoadenitis in an animal model for Sjogren's syndrome.Pathobiology 1996,64:269-274
[44]Fox RI,Kang Ho-Il,Ando D et al.Cytokine mRNA expression in salivary gland biopsies of Sjogren's syndrome.J Immunol 1994,152:5532-5540
[45]Baudouin C,De Saint Jean M,Brignole F et al.Expression of apoptotic and inflammatory markers in the human conjunctival epithelium.Invest Ophthalmol Vis Sci 1998,39:S551
[46]Baudouin C,Brignole F,Becquet F et al.Flow cytometry in impression cytology specimens:a new method for evaluation of conjunctival inflammation.Invest Ophthalmol Vis Sci 1997,38:1458-1464
[47]Brignole F,De Saint Jean M,Becquet F et al.Expression of Fas-Fas ligand antigens and apoptotic marker APO2.7 by the human conjunctival epithelium:positive correlation with class Ⅱ HLA-DR expression in inflammatory conditions.Exp Eye Res 1998;67:687-697
[48]肖林,胡静,等.水通道蛋白在干燥综合征患者唇腺中表达的实验研究[J】.临床口腔医学杂志 2004,20:457
[49]Price EJ,Venables PJ.The etiopathogenesis of Sjogren's syndrome.Semin Arthritis Rheum 1995;25:117-133
[50]Fox RI,Saito I.Sjogren's syndrome:immunologic and neuroendocrine mechanisms.Adv Exp Med Biol 1994,350:609-621
[51]Boehm U,Klamp T,Groot Met al.Cellular responses to interferon-gamma.Annu Rev Immunol 1997,15:749-795
[52]Mossman TR,Cherwinski HM,Bond MW et al.Two types of murine helper T cell clone,Ⅰ:definition according to profiles of lymphokine activities and secreted proteins.J Immunol 1986,136:2348-2357
[53]Romagnani S.Lymphokine production by human T cells in disease states.Annu Rev Immunol 1994,12:227-257
[54】吴翰桂,骆大法.IL-6负调控TH1细胞分化的分子机制.台州学院学报,2002,24(6):68
[55]Smith J K,Siddiqui AA,Modica L A,et al.Interferon-alpha upregulates gene expression of aquaporin-5 in human parotid glands[J].J Interferon Cytokine Res,1999,19(8):929-935
[1]Chisholm DM,Mason DK.Labial Salivary gland biopsy Sj(O|¨)gren's syndrome.J Clin Pathol,1996,76:427-430
[2]Greenspan JS,Daniels TE,Talal N.The histopatology of Sj(O|¨)gren's syndrome in labial Salivary gland biopsies.Oral Surg,1974,37:217
[3]Daniels TE,Whitcher JP.Association of patterns of labial Salivary gland in flammation with keratconjunctivitis sicca.Arthritis Rheum,1994,37:869
[4】陈慧颖,文竹咸,马正中.唇腺的年龄性改变及其在Sjogren综合征诊断中的意义[J].中国医学科学院学报,1993,15(5):343-348
[5】董怡.2002年修订的干燥综合征国际分类(诊断)标准[J].中华风湿病学杂志 2002;6:257
[6】李瑞琳,张凤山.第四届全国中西医结合风湿病学术会议纪要[J].中国中西结合杂志2001;21:157-159
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