虚燥更平散对更年期高血压病虚燥证模型大鼠血管重塑影响的实验研究
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摘要
高血压病是新疆常见病、高发病之一。高血压病患者在更年期这一特殊生理阶段,其血压波动性较大,临床降压药物疗效较差。导师黄永生教授通过多年的临床实践,基于“因地制宜”的原则,在临床上提出“肝肾阴虚,毒燥伤络”是新疆更年期高血压病发病关键,以地方药、民族药为组方的虚燥更平散治疗更年期高血压病疗效确切。本研究在前期研究的基础上,从文献综述、理论研究及实验研究方面对其进行深入、系统地探究和讨论。
文献综述
对更年期高血压病的病因病机、辨证分型、辨证论治以及实验研究等从中医学角度进行了系统的总结；对更年期高血压病的病因、诊断、发病机理、治疗现状及研究动态等从西医学角度进行了综述。
从中、西医学角度对高血压病、更年期高血压病及病证结合动物模型建立的实验研究进行了综述；对高血压病及更年期高血压病与血管重塑的发病机理、治疗现状及近年来药物干预的实验研究成果及研究动态进行了综述。
理论研究
基于“因地制宜”原则,阐述西北燥证对新疆更年期高血压病的病因病机、辨证分型、理法方药的影响,提出“肝肾阴虚,毒燥伤络”是其发病关键,从“虚燥论治”是治疗新疆更年期高血压病的基本方法,虚燥更平散是治疗更年期高血压病的有效方药,从而拓宽了中医药防治更年期高血压病的研究思路。
实验研究
1动物模型实验研究
1.1更年期高血压病动物模型复制的实验研究
将11月龄SHR大鼠32只随机分为SHR组、假手术组、更年期高血压病造模组,通过手术摘除SHR大鼠卵巢去势后复制更年期高血压病复合动物模型。采用ELISA法检测大鼠血清性激素水平,结果显示,与SHR组及假手术组比较,更年期高血压病造模组大鼠血清E2、T、P降低(P<0.05),血清FSH、LH升高(P<0.05)；在光镜下观察更年期高血压病造模组大鼠阴道涂片细胞学改变,其结果符合大鼠更年期特征,由此评价更年期高血压病复合大鼠模型复制成功。
1.2更年期高血压病虚燥证动物模型建立的实验研究
将已成模的11月龄更年期高血压病大鼠12只随机分为更年期高血压病组、更年期高血压病虚燥证造模组,并将更年期高血压病虚燥证造模组大鼠置于人工干燥环境中,给予西北燥证食物喂养,施加倒吊及外界铃声刺激,进一步建立更年期高血压病虚燥证动物模型,结果显示,与更年期高血压病组比较,更年期高血压病虚燥证造模组大鼠体重降低,饮水量增加,尿量减少(P<0.05)；采用ELISA法检测大鼠血清神经递质及肝功水平,结果显示,与更年期高血压病组比较,更年期高血压病虚燥证造模组大鼠血清CRH、NE、DA、5-HT、ALT、 AST、E2/T均升高,E2、T降低(P<0.05)；在光镜下观察大鼠肝脏、脾脏、肾上腺、下丘脑组织结构,其结果符合更年期高血压病虚燥证动物模型变化特点,由此评价更年期高血压病虚燥证动物模型的成功建立。
1.3更年期高血压病虚燥证对大鼠血管重塑影响的实验研究
将13月龄大鼠18只随机分为SHR组、更年期高血压病组、更年期高血压病虚燥证组,另设同月龄WKY组。结果显示,与WKY组比较,SHR组、更年期高血压病组、更年期高血压病虚燥证组大鼠血压水平升高,LD减少,MT、MT/LD增加(P<0.05),且各组大鼠血压水平的升高及腹主动脉血管重构程度呈现WKY组 2虚燥更平散对更年期高血压病虚燥证大鼠血管重塑的影响
将13月龄更年期高血压病虚燥证大鼠18只随机分为更年期高血压病虚燥证空白组、虚燥更平散组、缬沙坦组,分别灌服等体积蒸馏水、虚燥更平散、缬沙坦胶囊,干预60天后观察下列指标。
2.1虚燥更平散对更年期高血压病虚燥证大鼠生物表征、血压及腹主动脉组织结构的影响
虚燥更平散能抑制更年期高血压病虚燥证大鼠血压随年龄增长而升高的趋势,与更年期高血压病虚燥证空白组比较,虚燥更平散组大鼠血压在给药10天、20天时逐渐下降,在给药30天后有显著差异,且降压效果平稳持久；与更年期高血压病虚燥证空白组比较,虚燥更平散组大鼠腹主动脉LD增加,MT、MT/LD减少(P<0.05),揭示虚燥更平散通过滋阴润燥、养血通络作用改善更年期高血压病虚燥证大鼠虚燥征象,从而使其降压效果平稳持久,同时能改善更年期高血压病虚燥证大鼠血管内皮脱落以及平滑肌异常增生等病理性损害,由此逆转更年期高血压病虚燥证大鼠腹主动脉血管重构。
2.2虚燥更平散对更年期高血压病虚燥证大鼠基质金属蛋白酶及其抑制物的影响
本实验采用ELISA法分别检测各组大鼠血清MMP-9、TIMP-1水平,结果显示,与更年期高血压病虚燥证空白组比较,虚燥更平散组大鼠MMP-9、TIMP-1均降低(P<0.05),说明虚燥更平散能降低大鼠外周循环MMP-9水平,并使代偿性升高的TIMP-1降低,恢复MMP-9与TIMP-1动态平衡,减少其对血管基质的降解,从而逆转更年期高血压病虚燥证大鼠的血管重塑。
2.3虚燥更平散对更年期高血压病虚燥证大鼠TGF-β1及TGF-β1mRNA在血管中表达的影响
通过免疫组化法及RT-PCR方法检测各组大鼠TGF-β1及TGF-β1mRNA在血管中的表达,研究结果表明,虚燥更平散可能通过降低TGF-β1及TGF-β1mRNA在大鼠血管中的表达,实现对更年期高血压病虚燥证大鼠血管重塑的逆转,此结果也为从“虚燥论治”更年期高血压病的有效性提供了实验依据。
In the present, Essential Hypertension is one of the most common and the highest incidence disease in xinjiang. Essential Hypertension in the particular history of menopause can performance the fluctuation of blood pressure was larger and the stability of hypertensive drugs was worseer, Professor Huang Yongsheng puted forward the main bases of menopausal hypertension is the maladjustment of liver and kidney and the crux is the damage of superficial venues and lymph vessels by poison, which based on tthe principle of adjust measures to local reference review through many years clinical practices. Professor Huang believed the virtual dry treatment is a valid path in treatting menopausal hypertension and thinked Xu-zao-geng-ping powder has definite effect. The results of this study in order to further develop traditional Chinese medicine research lay the foundation for the prevention and control of menopause climacteric hypertension based on the previous heoretical research and clinical observation experimental research.
Reference review
The essay systematically summarized the advances of menopausal hypertension with TCM and experimental study as follows, the name of disease, the etiology and pathogenesis, syndrome differentiation with differentiation of symptoms and signs, different treatment with differentiation of symptoms and signs.
This Essay reviewed the experimental study results on hypertension experimental animal model, menopausal hypertension experimental animal model, menopausal hypertension yin-deficiency inner dryness syndrome experimental animal model.This Essay also summarized the principle of preventing and treating in menopausal hypertension according to the combine traditional Chinese and western medicine medical point and reviewed the pathogenesis and treatment of menopausal hypertension with vascular remodeling.
Theory study
The disease state was different because the different regions and times by putting the environmental factors into the medical model.This paper dicussed on the influence of northwest drying climate from three aspects:etiology and pathogenesis, syndrome, differentiation with differentiation of symptoms and signs, principle-method-recipe-medicines. We puted forward the main bases of menopausal hypertension was the maladjustment of liver and kidney and the crux was the damage of superficial venues and lymph vessels by poison, then putted forward the valid path of treating menopausal hypertension was "The virtual dry treatment". The application of xu-zao-geng-ping powder was effective agent in the treatment of climacteric hypertension, thus widening the research ideas of TCM prevention.
Experimental study
1The experimental research of animal model
1.1The experimental research of copy the menopause hypertension animal model
Randomly divided11months of32SHR rats into SHR group, control group, menopause hypertension model group, through the removal of SHR rats ovaries copy menopause hypertension animal model, using enzyme-linked immune (ELISA) method to detect the serum sex hormone (E2, FSH, LH, T, P) level, the results show that compare with SHR group and control group, the menopause hypertension rats serum E2, T, P decreased (P＜0.05), serum FSH and LH increased (P＜0.05). Made module rats under light microscopy observation of menopause hypertension rat vaginal smear cytology changes, the result conforms to the characteristics of menopausal rats, proves the ovaries, castration thoroughly, which can be evaluation copy successful copy menopause hypertension rat model.
1.2The experimental research of establish menopause hypertension Yin-deficiency with inner dryness syndrome experimental animal model
Randomly divided11months of12menopause hypertension rats into menopause hypertension group, menopause hypertension Yin-deficiency with inner dryness syndrome experimental animal model group, applying artificial dry environment and using the upside-down stimulation, while giving the northwest dryness syndrome food feeding, further establish the menopause hypertension Yin-deficiency with inner dryness syndrome experimental animal model. Compare with menopause hypertension rats, the weight of menopause hypertension Yin-deficiency with inner dryness syndrome experimental animal model rats lose, water quantity increased, urine volume decreased. Using ELISA method to detect the neurotransmitters and hepatic function of rats, the results show that compare with menopause hypertension rats, the CRH, NE, DA,5-HT, ALT and AST of menopause hypertension Yin-deficiency with inner dryness syndrome experimental animal model group were higher, serum E2, T, E2/T score were lower, the result accord with dryness syndrome animal model evaluation related index changes, then to observe rat liver, spleen, adrenal gland, hypothalamus, histomorphology in line with the characteristic of virtual dryness syndrome animal model changes, which can evaluate menopause hypertension Yin-deficiency with inner dryness syndrome experimental animal model is successfully established.
1.3The experimental research of menopause hypertension Yin-deficiency with inner dryness syndrome experimental animal model with vascular remodeling rats
The only13months of age18rats were randomly divided into SHR and menopause hypertension group, menopause hypertension Yin-deficiency with inner dryness syndrome group, the WKY group. Results show that compared with WKY group, SHR group, menopause hypertension group, the menopause hypertension Yin-deficiency with inner dryness syndrome group rats blood pressure levels, LD, MT and MT/LD increased (P＜0.05); Using ELISA method to detect the rat serum levels of MMP-9and TIMP-1level, using immunohistochemical method and RT-PCR detection rat TGF-β1and TGF-β1 mRNA expression in blood vessels, the research results show that compared with WKY group、SHR group、menopause hypertension group,the menopause hypertension Yin-deficiency with inner dryness syndrome group imbalance is aggravating, TGF-β1and TGF-β1mRNA expression in blood vessels, thus speculated that the above two aspects may be menopause hypertension virtual dryness syndrome occurrence and development of vascular remodeling in rats is one of the pathological mechanism, TGF-β1and TGF-β1mRNA expression might be poisonous dry injury is caused by menopause hypertension virtual dryness syndrome is one of the characteristics of vascular remodeling in rats.
2The Effects of Xu-zao-geng-ping powder on vascular remodeling in menopause hypertension Yin-deficiency with inner dryness syndrome experimental animal model
The13months of menopause hypertension Yin-deficiency with inner dryness syndrome experimental animal model rats18were randomly divided into menopause hypertension Yin-deficiency with inner dryness syndrome black group, Xu-zao-geng-ping powder group and valsartan group. Respectively to the irrigation uniform such as distilled water volume more flat, Xu-zao-geng-ping powder and valsartan capsule after60days, observe the following indicators.2.1The effect of Xu-zao-geng-ping powder on biological characterization in rats, the impact of blood pressure and vascular structure in menopause hypertension Yin-deficiency with inner dryness syndrome experimental animal model
Xu-zao-geng-ping powder can inhibit menopause hypertension Yin-deficiency with inner dryness syndrome experimental animal blood pressure with age and the rising trend, and menopause hypertension after30days from the drug virtual dry the blank group comparison, there were significant differences, and antihypertensive effect smooth and durable compare with menopause hypertension Yin-deficiency with inner dryness syndrome blank group. The Xu-zao-geng-ping powder can raise LD value, low MT (P＜0.05), improve the dry signs through nourishing Yin for moistening dryness, nourishing blood for dredging collaterals, so as to make it smooth step-down lasting, at the same time can improve the vessels tissue injury in Yin-deficiency with inner dryness syndrome experimental animal model rats.
2.2The effect of Xu-zao-geng-ping powder on MMP-9, TIMP-1in menopause hypertension Yin-deficiency with inner dryness syndrome experimental animal model
This experiment adopts the enzyme-linked method between groups of rats was detected in serum level of MMP-9and TIMP-1. Results show that comparing with menopause hypertension Yin-deficiency with inner dryness syndrome blank group, the MMP-9and TIMP-1values of Xu-zao-geng-ping powder group are lower (P＜0.05), shows that Xu-zao-geng-ping powder can reduce rat peripheral circulation MMP-9level, lower the compensatory increase of TIMP-1, MMP-9and TIMP-1dynamic balance, reduce its degradation of vascular matrix, thereby reversing climacteric hypertension dryness syndrome of vascular remodeling in rats.
2.3The effect of xu-zao-geng-ping powder on TGF-β1in abdominalis aorta tissue and TGF-β1mRNA in thoracic aorta tissue of menopause hypertension Yin-deficiency with inner dryness syndrome experimental animal model
By immune-histochemical method and RT-PCR method for detection of each rat TGF-β1expression and TGF-β1mRNA expression in ratery tissue. Results show that Xu-zao-geng-ping powder may adjust the TGF-β1and TGF-β1mRNA expression in ratery tissue, relieve menopause hypertension virtual dry blood vessel tissue injury in rats, the implementation of menopause hypertension virtual dry reversal of vascular remodeling in rats, the results for "virtual dry treatment" menopause climacteric hypertension virtual effectiveness of dry syndrome in rats provides experiment basis.
文献综述
对更年期高血压病的病因病机、辨证分型、辨证论治以及实验研究等从中医学角度进行了系统的总结；对更年期高血压病的病因、诊断、发病机理、治疗现状及研究动态等从西医学角度进行了综述。
从中、西医学角度对高血压病、更年期高血压病及病证结合动物模型建立的实验研究进行了综述；对高血压病及更年期高血压病与血管重塑的发病机理、治疗现状及近年来药物干预的实验研究成果及研究动态进行了综述。
理论研究
基于“因地制宜”原则,阐述西北燥证对新疆更年期高血压病的病因病机、辨证分型、理法方药的影响,提出“肝肾阴虚,毒燥伤络”是其发病关键,从“虚燥论治”是治疗新疆更年期高血压病的基本方法,虚燥更平散是治疗更年期高血压病的有效方药,从而拓宽了中医药防治更年期高血压病的研究思路。
实验研究
1动物模型实验研究
1.1更年期高血压病动物模型复制的实验研究
将11月龄SHR大鼠32只随机分为SHR组、假手术组、更年期高血压病造模组,通过手术摘除SHR大鼠卵巢去势后复制更年期高血压病复合动物模型。采用ELISA法检测大鼠血清性激素水平,结果显示,与SHR组及假手术组比较,更年期高血压病造模组大鼠血清E2、T、P降低(P<0.05),血清FSH、LH升高(P<0.05)；在光镜下观察更年期高血压病造模组大鼠阴道涂片细胞学改变,其结果符合大鼠更年期特征,由此评价更年期高血压病复合大鼠模型复制成功。
1.2更年期高血压病虚燥证动物模型建立的实验研究
将已成模的11月龄更年期高血压病大鼠12只随机分为更年期高血压病组、更年期高血压病虚燥证造模组,并将更年期高血压病虚燥证造模组大鼠置于人工干燥环境中,给予西北燥证食物喂养,施加倒吊及外界铃声刺激,进一步建立更年期高血压病虚燥证动物模型,结果显示,与更年期高血压病组比较,更年期高血压病虚燥证造模组大鼠体重降低,饮水量增加,尿量减少(P<0.05)；采用ELISA法检测大鼠血清神经递质及肝功水平,结果显示,与更年期高血压病组比较,更年期高血压病虚燥证造模组大鼠血清CRH、NE、DA、5-HT、ALT、 AST、E2/T均升高,E2、T降低(P<0.05)；在光镜下观察大鼠肝脏、脾脏、肾上腺、下丘脑组织结构,其结果符合更年期高血压病虚燥证动物模型变化特点,由此评价更年期高血压病虚燥证动物模型的成功建立。
1.3更年期高血压病虚燥证对大鼠血管重塑影响的实验研究
将13月龄大鼠18只随机分为SHR组、更年期高血压病组、更年期高血压病虚燥证组,另设同月龄WKY组。结果显示,与WKY组比较,SHR组、更年期高血压病组、更年期高血压病虚燥证组大鼠血压水平升高,LD减少,MT、MT/LD增加(P<0.05),且各组大鼠血压水平的升高及腹主动脉血管重构程度呈现WKY组
将13月龄更年期高血压病虚燥证大鼠18只随机分为更年期高血压病虚燥证空白组、虚燥更平散组、缬沙坦组,分别灌服等体积蒸馏水、虚燥更平散、缬沙坦胶囊,干预60天后观察下列指标。
2.1虚燥更平散对更年期高血压病虚燥证大鼠生物表征、血压及腹主动脉组织结构的影响
虚燥更平散能抑制更年期高血压病虚燥证大鼠血压随年龄增长而升高的趋势,与更年期高血压病虚燥证空白组比较,虚燥更平散组大鼠血压在给药10天、20天时逐渐下降,在给药30天后有显著差异,且降压效果平稳持久；与更年期高血压病虚燥证空白组比较,虚燥更平散组大鼠腹主动脉LD增加,MT、MT/LD减少(P<0.05),揭示虚燥更平散通过滋阴润燥、养血通络作用改善更年期高血压病虚燥证大鼠虚燥征象,从而使其降压效果平稳持久,同时能改善更年期高血压病虚燥证大鼠血管内皮脱落以及平滑肌异常增生等病理性损害,由此逆转更年期高血压病虚燥证大鼠腹主动脉血管重构。
2.2虚燥更平散对更年期高血压病虚燥证大鼠基质金属蛋白酶及其抑制物的影响
本实验采用ELISA法分别检测各组大鼠血清MMP-9、TIMP-1水平,结果显示,与更年期高血压病虚燥证空白组比较,虚燥更平散组大鼠MMP-9、TIMP-1均降低(P<0.05),说明虚燥更平散能降低大鼠外周循环MMP-9水平,并使代偿性升高的TIMP-1降低,恢复MMP-9与TIMP-1动态平衡,减少其对血管基质的降解,从而逆转更年期高血压病虚燥证大鼠的血管重塑。
2.3虚燥更平散对更年期高血压病虚燥证大鼠TGF-β1及TGF-β1mRNA在血管中表达的影响
通过免疫组化法及RT-PCR方法检测各组大鼠TGF-β1及TGF-β1mRNA在血管中的表达,研究结果表明,虚燥更平散可能通过降低TGF-β1及TGF-β1mRNA在大鼠血管中的表达,实现对更年期高血压病虚燥证大鼠血管重塑的逆转,此结果也为从“虚燥论治”更年期高血压病的有效性提供了实验依据。
In the present, Essential Hypertension is one of the most common and the highest incidence disease in xinjiang. Essential Hypertension in the particular history of menopause can performance the fluctuation of blood pressure was larger and the stability of hypertensive drugs was worseer, Professor Huang Yongsheng puted forward the main bases of menopausal hypertension is the maladjustment of liver and kidney and the crux is the damage of superficial venues and lymph vessels by poison, which based on tthe principle of adjust measures to local reference review through many years clinical practices. Professor Huang believed the virtual dry treatment is a valid path in treatting menopausal hypertension and thinked Xu-zao-geng-ping powder has definite effect. The results of this study in order to further develop traditional Chinese medicine research lay the foundation for the prevention and control of menopause climacteric hypertension based on the previous heoretical research and clinical observation experimental research.
Reference review
The essay systematically summarized the advances of menopausal hypertension with TCM and experimental study as follows, the name of disease, the etiology and pathogenesis, syndrome differentiation with differentiation of symptoms and signs, different treatment with differentiation of symptoms and signs.
This Essay reviewed the experimental study results on hypertension experimental animal model, menopausal hypertension experimental animal model, menopausal hypertension yin-deficiency inner dryness syndrome experimental animal model.This Essay also summarized the principle of preventing and treating in menopausal hypertension according to the combine traditional Chinese and western medicine medical point and reviewed the pathogenesis and treatment of menopausal hypertension with vascular remodeling.
Theory study
The disease state was different because the different regions and times by putting the environmental factors into the medical model.This paper dicussed on the influence of northwest drying climate from three aspects:etiology and pathogenesis, syndrome, differentiation with differentiation of symptoms and signs, principle-method-recipe-medicines. We puted forward the main bases of menopausal hypertension was the maladjustment of liver and kidney and the crux was the damage of superficial venues and lymph vessels by poison, then putted forward the valid path of treating menopausal hypertension was "The virtual dry treatment". The application of xu-zao-geng-ping powder was effective agent in the treatment of climacteric hypertension, thus widening the research ideas of TCM prevention.
Experimental study
1The experimental research of animal model
1.1The experimental research of copy the menopause hypertension animal model
Randomly divided11months of32SHR rats into SHR group, control group, menopause hypertension model group, through the removal of SHR rats ovaries copy menopause hypertension animal model, using enzyme-linked immune (ELISA) method to detect the serum sex hormone (E2, FSH, LH, T, P) level, the results show that compare with SHR group and control group, the menopause hypertension rats serum E2, T, P decreased (P＜0.05), serum FSH and LH increased (P＜0.05). Made module rats under light microscopy observation of menopause hypertension rat vaginal smear cytology changes, the result conforms to the characteristics of menopausal rats, proves the ovaries, castration thoroughly, which can be evaluation copy successful copy menopause hypertension rat model.
1.2The experimental research of establish menopause hypertension Yin-deficiency with inner dryness syndrome experimental animal model
Randomly divided11months of12menopause hypertension rats into menopause hypertension group, menopause hypertension Yin-deficiency with inner dryness syndrome experimental animal model group, applying artificial dry environment and using the upside-down stimulation, while giving the northwest dryness syndrome food feeding, further establish the menopause hypertension Yin-deficiency with inner dryness syndrome experimental animal model. Compare with menopause hypertension rats, the weight of menopause hypertension Yin-deficiency with inner dryness syndrome experimental animal model rats lose, water quantity increased, urine volume decreased. Using ELISA method to detect the neurotransmitters and hepatic function of rats, the results show that compare with menopause hypertension rats, the CRH, NE, DA,5-HT, ALT and AST of menopause hypertension Yin-deficiency with inner dryness syndrome experimental animal model group were higher, serum E2, T, E2/T score were lower, the result accord with dryness syndrome animal model evaluation related index changes, then to observe rat liver, spleen, adrenal gland, hypothalamus, histomorphology in line with the characteristic of virtual dryness syndrome animal model changes, which can evaluate menopause hypertension Yin-deficiency with inner dryness syndrome experimental animal model is successfully established.
1.3The experimental research of menopause hypertension Yin-deficiency with inner dryness syndrome experimental animal model with vascular remodeling rats
The only13months of age18rats were randomly divided into SHR and menopause hypertension group, menopause hypertension Yin-deficiency with inner dryness syndrome group, the WKY group. Results show that compared with WKY group, SHR group, menopause hypertension group, the menopause hypertension Yin-deficiency with inner dryness syndrome group rats blood pressure levels, LD, MT and MT/LD increased (P＜0.05); Using ELISA method to detect the rat serum levels of MMP-9and TIMP-1level, using immunohistochemical method and RT-PCR detection rat TGF-β1and TGF-β1 mRNA expression in blood vessels, the research results show that compared with WKY group、SHR group、menopause hypertension group,the menopause hypertension Yin-deficiency with inner dryness syndrome group imbalance is aggravating, TGF-β1and TGF-β1mRNA expression in blood vessels, thus speculated that the above two aspects may be menopause hypertension virtual dryness syndrome occurrence and development of vascular remodeling in rats is one of the pathological mechanism, TGF-β1and TGF-β1mRNA expression might be poisonous dry injury is caused by menopause hypertension virtual dryness syndrome is one of the characteristics of vascular remodeling in rats.
2The Effects of Xu-zao-geng-ping powder on vascular remodeling in menopause hypertension Yin-deficiency with inner dryness syndrome experimental animal model
The13months of menopause hypertension Yin-deficiency with inner dryness syndrome experimental animal model rats18were randomly divided into menopause hypertension Yin-deficiency with inner dryness syndrome black group, Xu-zao-geng-ping powder group and valsartan group. Respectively to the irrigation uniform such as distilled water volume more flat, Xu-zao-geng-ping powder and valsartan capsule after60days, observe the following indicators.2.1The effect of Xu-zao-geng-ping powder on biological characterization in rats, the impact of blood pressure and vascular structure in menopause hypertension Yin-deficiency with inner dryness syndrome experimental animal model
Xu-zao-geng-ping powder can inhibit menopause hypertension Yin-deficiency with inner dryness syndrome experimental animal blood pressure with age and the rising trend, and menopause hypertension after30days from the drug virtual dry the blank group comparison, there were significant differences, and antihypertensive effect smooth and durable compare with menopause hypertension Yin-deficiency with inner dryness syndrome blank group. The Xu-zao-geng-ping powder can raise LD value, low MT (P＜0.05), improve the dry signs through nourishing Yin for moistening dryness, nourishing blood for dredging collaterals, so as to make it smooth step-down lasting, at the same time can improve the vessels tissue injury in Yin-deficiency with inner dryness syndrome experimental animal model rats.
2.2The effect of Xu-zao-geng-ping powder on MMP-9, TIMP-1in menopause hypertension Yin-deficiency with inner dryness syndrome experimental animal model
This experiment adopts the enzyme-linked method between groups of rats was detected in serum level of MMP-9and TIMP-1. Results show that comparing with menopause hypertension Yin-deficiency with inner dryness syndrome blank group, the MMP-9and TIMP-1values of Xu-zao-geng-ping powder group are lower (P＜0.05), shows that Xu-zao-geng-ping powder can reduce rat peripheral circulation MMP-9level, lower the compensatory increase of TIMP-1, MMP-9and TIMP-1dynamic balance, reduce its degradation of vascular matrix, thereby reversing climacteric hypertension dryness syndrome of vascular remodeling in rats.
2.3The effect of xu-zao-geng-ping powder on TGF-β1in abdominalis aorta tissue and TGF-β1mRNA in thoracic aorta tissue of menopause hypertension Yin-deficiency with inner dryness syndrome experimental animal model
By immune-histochemical method and RT-PCR method for detection of each rat TGF-β1expression and TGF-β1mRNA expression in ratery tissue. Results show that Xu-zao-geng-ping powder may adjust the TGF-β1and TGF-β1mRNA expression in ratery tissue, relieve menopause hypertension virtual dry blood vessel tissue injury in rats, the implementation of menopause hypertension virtual dry reversal of vascular remodeling in rats, the results for "virtual dry treatment" menopause climacteric hypertension virtual effectiveness of dry syndrome in rats provides experiment basis.
引文
[1]Lopez AD Assessing the burden of mortality from cardiovascular disease[J]. World Health Stat Q,1993,46:91-96.
[2]Ezzati M, Lopez AD, Rodgers A, et al. Selected major risk factors and global and negional burden of disease[J]. Lancet,2002,360(9343):1347-1360.
[3]朱宝珍,范世慧.二仙汤加减对更年期妇女高血压患者生活质量的影响[J].河北中医,2007,29(1)：80-81.
[4]尚玉红,林雪.更年期高血压中医研究进展[J].中国中医药杂志,2007,5(8)：42-44.
[5]姚憬.更年期高血压之辨证施治[J].浙江中医杂志,2007,42(2)：110.
[6]周慧成.围绝经期动态血压变异性探讨[J].中国社区医师,2004,6(14)：67-68.
[7]魏相玲.更年期综合征的中医辨证治疗[J].光明中医,2008(6)：847-848.
[8]常惠,陈启光,闵婕.更年期综合征中医辨证现代化研究现场调研总结[J].浙江中医杂志,2004,(3)：100-103.
[9]李炳文,胡雪桔.浅谈女性更年期高血压病的防治[J].中医药临床杂志,2007,19(4)：336-337.
[10]高文军,杨国强.二仙汤加减治疗女性更年期高血压51例[J].陕西中医,2008,29(7)：784-785.
[11]刘平,崔卓敏.逍遥散联合西药治疗女性围绝经期原发性高血压59例[J].陕西中医,2009,30(7)：789-790.
[12]卢跃棣,叶开升.替米沙坦合更年安片治疗更年期高血压[J].浙江中西医结 合杂志,2007,17(2)：110-111.
[13]陶颖.中西医结合治疗绝经期女性合并高血压33例[J].中医药信息,2007,24(3)：52-53.
[14]叶盈,黄飞翔,王永等.六味地黄丸合络活喜治疗女性更年期高血压疗效观察[J].中国中医急症,2006,15(5)：487-488.
[15]李志,解发良.中西医结合并心理治疗女性高血压合并更年期综合征58例临床观察[J].中国中医药科技,2006,27(12)：137-138.
[16]周志昆,曾红兵,高植明.中药及复方调节神经内分泌免疫网络研究进展[J].湖南中医杂志,2006,22(2)：89.
[17]曾燕静,潘竞霞,刘柯兵等.温胆片治疗痰浊型高血压患者颈动脉粥样硬化的血流动力改变[J].内蒙古中医药,2008(2)：10.
[18]许娜,成丽.围绝经期定义[J].中国实用乡村医生杂志,2008,15(8)：1-3.
[19]古萍,王庆.血浆儿茶酚胺对血液透析患者高血压的影响[J].实用医学杂志,2004,20(3)：263-264.
[20]邱一华,彭聿平,王建军.儿茶酚胺调节免疫功能的细胞和分子机制[J].生理科学进展,2003,22(4)：16-21.
[21]汤健,唐朝枢,循环系统的内分泌功能[M].北京：北京医科大学、中国协和医科大学联合出版社,1989：121-124.
[22]袁公贤,哈黛文.多巴胺受体D1基因-48A/G多态性与原发性高血压病的关联研究[J].高血压杂志,2002(10)3：249-251.
[23]刘养凤,张军平等.冰片对大鼠血浆儿茶酚胺类物质影响的实验研究[J].天津中医药,2004,21(2)：58-59.
[24]Posnik-Urbanska A, Kawecka-jaszcz K. HyPertension in PostmenoPausal women-selected Pathome chanisms[J]. Przegl Lek,2006,63(12):1313-1317.
[25]郭雪微,王雪里红,高志凌.血浆神经肽Y神经降压肽水平与高血压患者血压昼夜变化和靶器官损害的研究[J].中华心血管病杂志,2005,33(11)：1006-1009.
[26]向菲,毛华.性激素与胰岛素抵抗关系在女性更年期高血压发病中的作用[J].贵州医药,2000,24(4)：237.
[27]Mendelsohn ME.Protective effects of estrogen on the cardiovascular system[J].Am J Cardiol,2002,89(supply):12E-18E.
[28]Chen Z, Yuhanna IS, Calcheva-Cargowa Z, et al. Eestogen receptor alpha mediates the non-genomic activation of endothelial nitric oxide synthase by estrogen[J]. Clin Invest,1999,325:756-762.
[29]张苏明,江红,何池中,等.更年期与阿尔茨海默病[J].国外医学,2001,28(2)：118.
[30]朱海燕,赖爱鸾,张李松.激素替代治疗联合降压药物对围绝经期原发性高血压妇女血压及内皮功能的影响[J].中国全科医学,2008,11(8)：1448-1450.
[1]崔慧慧,杨建一,李莉.不同类型高血压模型的研究进展[J].热带医学杂志,2008,58(5)：509-511.
[2]Liu K L, Lo M, Jean S. Lyon genetically hypertensive rats:an animal model of "low renin hypertension"[J]. Acta Pharmacologica Sinica,2003,24(1):1-6.
[3]范平,司军强,刘政江,等.自发性高血压大鼠模型的应用研究进展[J].农垦医学,2004,26(6)：444-448.
[4]孙艳红,金若敏.女性更年期综合征动物模型制备的研究进展[J].时珍国医药,2005,6(7)：657-659.
[5]赖远征,冯冰虹,刘美珍,等.补肾活血方对更年期大鼠性激素的影响[J].辽宁中医杂志,2004,31(1)：78-79.
[6]鲁遂荣,方学楹,侯安继,等.更年平调液对更年期大鼠自由基影响的实验研究[J].中国中医基础医学杂志,1998,4(8)：35-38.
[7]李仪奎.中药药理实验方法学(第二版)[M].上海：上海科学技术出版社,2006：831-839.
[8]李鸿霞.虚燥更平散对更年期高血压大鼠血管组织形态学及MMP-9、TIMP-1影响的实验研究[D].新疆医科大学,2012年,26-30.
[9]甘庆雷.虚燥更平散对更年期高血压大鼠血压及血压变异性影响的实验研究[D].新疆医科大学,2012年,26-27.
[10]陈小野.证候动物模型诊断依据的设想与评价[J].中国医药学报,1987,2(1)：50.
[11]周铭心.西北燥证研究概述[J].上海中医药杂志,2005,39(11)：43-45.
[12]白泊,高锡瑞,徐根才,等.中国地图集[M].北京：中国地图出版社,2006年版.
[13]沙塔娜提,孙红友,周铭心.环境地理因素与亚健康状态的关系初探-西北燥证背景研究[J].新疆中医药,2006,24(4)：74-75.
[14]Kelsey RM, Alpert BS, Patterson SM, et al. Radial differences in hemody namic responses to environmental thermal stress among ado escents[J]. Circulation,2000,01:2284-2289.
[15]吕光耀,李杰,周铭心.新疆不同地区不同民族居民生存质量与西北燥证关 系的分析[J].新疆医科大学学报,2007,30(1)：6-10.
[16]牟全胜,周铭心.西北多燥说[J].新疆中医药,1991,9(4)：1.
[17]高华,沙塔娜提·穆罕默德,周铭心.西北燥证与心理素质关系探讨[J].新疆医科大学学报,2007,30(1)：17-19.
[18]周铭心,宋晓平,单丽娟,等.新疆各地不同民族居民西北燥证罹患情况流行病学调查分析[J].新疆医科大学学报,2006,29(11)：1034-1038.
[19]傅益群,吕健.动物模型与中医证候关系释义[J].中医药学刊,2004,22(9)：1666.
[20]何佳,李鹏,周铭心.西北燥证动物模型设想[J].时珍国医国药,2008,19(3)：529.
[21]丁建中,张六通,邱幸凡.外燥之理论研究探析[J].中国中医基础医学杂志,2005,11(10)：724.
[22]哈木拉提·吾甫尔,阿依努尔·买提斯迪克,努尔买买提·艾买提,等.异常黑胆质证载体动物模型的建立及其自然恢复反证[J].新疆医科大学学报,2006,29(10)：910-914.
[23]高振,阿地力江·阿布力米提,哈木拉提·吾甫尔.干燥寒冷环境对小鼠生物表征的影响[J].科学导报,2008,26(14)：84-87.
[24]高振,阿地力江·阿布力米提,胡汉华.西北寒燥证证候动物模型的生物学基础研究[J].科技导报,2009,27(2)：88.
[25]高振,阿地力,哈木拉提·吾甫尔.西北寒燥证证候模型免疫功能紊乱的研究[J].中华中医药杂志,2010,25(8)：1225-1228.
[26]高振,阿地力江,哈木拉提·吾甫尔.西北寒燥证模型与IL-2、IL-6、INF-y、TNF-α关系研究[J].时珍国医国药,2010,21(12)：3305-3307.
[27]杨翠平.温热药造小鼠阴虚模型的建立及其对小鼠抗氧化作用的影响[J].四川中医,2004,10(11)：15.
[28]王秋.家兔温病阴虚热盛证动物模型[J].辽宁中医杂志,1995,22(9)：427-429.
[29]汪泳涛,张伟荣,宋红普,等.长期超负荷游泳运动建立阴虚内热证动物模型的研究[J].中医药学刊,2003,21(7)：1115-1142.
[30]凌昌全,李敏,朱德增,等.阴阳虚证与糖皮质激素受体关系的临床与实验研究[J].浙江中西医结合杂志,2001,11(9)：532-533.
[31]夏洪生,张永锋,刘立昌,等.归蓉补血片对小鼠阴虚证模型作用的实验研究[J].湖北中医学院学报,2001,3(2)：23.
[32]张丽萍,杜彩霞.两地汤煎剂对拟更年期阴虚内热大鼠模型的影响[J].中医研究,2007,2(8)：25-26.
[33]樊蔚虹.长期激怒法造模[J].中国中医基础医学杂志,2001,7(9)：68-69.
[34]任小巧,卢跃卿,邓伟,等.加味一贯煎对实验性肝肾阴虚证大鼠下丘脑-垂体-甲状腺轴的影响[J].中国中药志,2000,25(3)：172-174.
[35]方肇勤,潘志强,卢文丽,等.小鼠常见证候的辨证标准[J].中国中医基础医学杂志,2006,12(7)：508-510.
[36]郑春叶,黄燕.中医证候动物模型复制管见[J].中华中医药学刊,2007,25(5)：914.
[37]龚婕宁,杨进.家兔病毒性肺热证模型的建立[J].中国中医基础医学杂志,1995,1(3)：46-48.
[38]彭成.脾气虚脾不统血证候动物模型的研究思路[J].中医杂志,1996,37(4)：241.
[1]朱宝珍,范世慧.二仙汤加减对更年期妇女高血压患者生活质量的影响[J].河北中医,2007,29(1)：80-81.
[2]翟丽华.高血压血管重塑及药物干预后的逆向重塑[J].高血压杂志,2001(2)：76-80.
[3]李鸿霞.虚燥更平散对更年期高血压大鼠血管组织形态学及MMP-9、 TIMP-1影响的实验研究[D].新疆医科大学,2012年,26-30.
[4]甘庆雷.虚燥更平散对更年期高血压大鼠血压及血压变异性影响的实验研究[D].新疆医科大学,2012年,26-27.
[5]Hope D, Intenganrnestol, Schiffrin. Vascular Remodeling in Hypertension: Role of Apoptosis, Inflammation and Fibrosis[J]. Hypertension,2001,38: 581-587.
[6]Bussy C, Bouyrie P, Lacolley P, et al. Intrinsic stiffs soft hearted arterial wall arterial inessential hypertensives[J]. Hypertension,2000,35(5):1049-1054.
[7]Dumonto, Loufrani L, Henrion D. Keyrole of the Npathway and matrix metal Protease-9 in high blood flow-induced remodeling of rat resorted near tries[J]. Arteriosler Thromb Vase Biol,2007,27(2):317-324.
[8]徐鹏宵,李红,孙昭英.自发性高血压大鼠心肌血管内皮生长因子表达水平的检测[J].武警医学,2005,6(7)：491-493.
[9]Bobik A. Transforming growth factor betas and vascular disorders[J]. Arterioscler Thromb Vasc Biol,2006,26:1712-1720.
[10]Park J, Ha H, Ahn HJ, et al. Sirolimus inhibits platelet-derived growth factor-induced collagen synthesis in rat vascular smooth muscle cells[J]. Transplant Proc,2005,37:3459-3462.
[11]胡锦生,乔木.内皮功能与心血管疾病[J].岭南心血管病杂 志,2000,6：327-331.
[12]Kenneth MY. Fibroin acting peptides in cell migration and wound repair[J]. Clini Invest,2000,105(11):1507-1509.
[13]李庆平,陆泽安,绕曼人.粉防己碱抑制VSMC胶原合成[J].药学学报,2001,36(7)：481-484.
[14]白江涛.基质金属蛋白酶与心血管疾病[J].心血管病学进展,2003,24(1)：43-47.
[15]Intengan HD, Schiffrin EL. Vaseular Remodeling in Hypertension. Roles of Apoptosis, Inflammation and Fibrosis[J]. Hypertension,2001,38:581-587.
[16]Ergul A, Portik-Dobos V, Hutchinson J, et al. Down regulation of vascular matrix metalloproteinase inducer and activator proteins in hypertensive patients[J]. Am J Hypertens,2004,17:775-782.
[17]张丽芳,柯元南.大鼠动脉内膜损伤后血管重塑相关因子的动态变化[J].中日友好医院学报,2007,21：91-93.
[18]Peterson JT, Li H, Dillon L, et al. Evolution of matrix metal proteins and tissue inhibitor expression during heart failure progression in the infracted rat[J]. Cardio vase Res,2000,46(2):307-315.
[19]Li H, Simon H, Bocan TM, et al. MMP/TIMP expression in spontaneously hypertensive heart failure rats:the effect of ACE-and MMP-inhibition[J]. Cardio vase Res,2000,46:298-306.
[20]Begona Lopez, Arantxa Gonzalez,Ram6n Querejeta, et al. Alterations in Pattern of Collagen Deposition May Contribute to the Deterioration of Systolic Function in Hypertensive Patienis with Heart Failure[J]. Am Coll Cardiol,2006,89-96.
[21]徐贤雄,欧雅莉,杨天伦.氨氯地平与厄贝沙坦对原发性高血压患者血清基质金属蛋白酶的影响[J].中国现代医学杂志,2010,20(14)：2201-2203.
[22]周虹,邢之华,刘卫平,等.动态血压分析天麻钩藤饮的降压效果[J].湖南中医学院学报,2005,25(4)：40-41.
[23]曾燕静,潘竞霞,刘柯兵,等.温胆片治疗痰浊型高血压患者颈动脉粥样硬化的血流动力改变[J].内蒙古中医药,2008(2)：10.
[24]王华军,谢良地,姚恩辉,等.通心络对自发性高血压大鼠阻力血管结构的影响[J].高血压杂志,2006,14(1)：61-63.
[25]史载祥.后再灌注时代难题的中西医结合治疗思考[J].中西医结合心脑血管病杂志,2005,3(1)：2-5.
[26]徐凤芹,徐浩,刘剑刚,等.芎芍胶囊对动脉粥样硬化兔血管平滑肌细胞增殖的影响[J].中国中西医结合杂志,2008,28(10)：912.
[27]徐凤芹,徐浩,马鲁波,等.芎芍胶囊对动脉粥样硬化兔血管平滑肌细胞凋亡的影响[J].中西医结合心脑血管病杂志,2008,6(7)：794.
[28]余胜,董建勋,郝钰,等.舒脉胶囊对Ang Ⅱ诱导的血管平滑肌细胞增殖及细胞周期的影响[J].北京中医药大学学报,2009,32(3)：178.
[29]翟丽华.高血压血管重塑及药物干预后的逆向重塑[J].高血压杂志,2001(2)：76-80.
[30]车在前,顾天华,吴永杰,等.苯丙氨酸缓解高血压大鼠血管重塑[J].中国病理生理杂志,2005,21(2)：327-330.
[1]周春绵,王陆建.血压变异性的机制和临床意义[J].临床荟萃,2003,18(11)：657-658.
[2]沙塔娜提,孙红友,周铭心.环境地理因素与亚健康状态的关系初探-西北燥证背景研究[J].新疆中医药,2006,24(4)：74-75.
[3]周铭心.西北燥证研究概述[J].上海中医药杂志,2005,39(11)：43-45.
[4]吕光耀,李杰,周铭心.新疆不同地区不同民族居民生存质量与西北燥证关系的分析[J].新疆医科大学学报,2007,30(1)：6-10.
[5]林雪,尚玉红,马丽,等.环境地理因素与更年期高血压的关系探微[J].中华中医药,2008,23(2)：125-127.
[6]林雪,余承云,雷震云,等.女性更年期高血压阴虚内燥证和非阴虚内燥证与性激素及血脂血糖水平的相关性研究[J].中国中西医结合杂志,2009,29(7)：603-606.
[7]吕光耀,李杰,周铭心.燥证及西北燥证病因病机研究概述[J].新疆医科大学学报,2007,30(1)：26-28.
[8]李南方,周玲,曹梅,等.新疆和丰县牧民高血压病流行病学调查报告[J].现代预防医学,2003,30(2)：141-143.
[9]林雪,雷震云,余承云,等.虚燥更平颗粒对女性更年期高血压血压变异性的影响[J].新疆医科大学学报,2008,31(12)：1753-1756.
[10]林雪,尚玉红,杨娜.刘继祖治疗更年期女性高血压病经验[J].新疆中医药,2006,24(2)：37-38.
[11]杨晓钊.中西医结合治疗妇女更年期高血压60例疗效观察[J].广西医科大学报,2001,18(4)：561-562.
[12]罗元凯.实用中医妇科学[M].上海：上海科学技术出版社,1994,113.
[13]雷燕.络病理论探微[J].北京中医药大学学报,1998,21(2)：18-23.
[14]杨传华,陆峰,张翠英.高血压血管重塑与络病的相关性[J].山东中医杂志,2005,11(24)：643-646.
[15]吴以岭.络病与血管病变的相关性研究及治疗[J].中医杂志,2006,47(3)：163-165.
[16]安乐君,葛丽,林雪.黄永生治疗更年期高血压病经验探微[J].中国实验方剂学杂志,2012,24(18)：360-362.
[17]秦长林.燥证琐谈[J].湖北中医杂志,2001,23(8)：6-7.
[18]王芳,张亚宏,左代英,等.红人归胶囊治疗更年期综合征作用机制初探[J].沈阳药科大学学报,2008,25(9)：739-742.
[19]马丽,林雪.虚燥更平颗粒对阴虚内燥证女性高血压病(围绝经期)患者生活质量的影响[J].中华中医药杂志,2012,27(3)：547-551.
[20]林雪,马丽,李红霞.虚燥更平散治疗围绝经期高血压病的临床观察[J].中华中医药杂志,2012,27(11)：2986-2988.
[21]何佳,迟新栋,林雪.虚燥更平散治疗围绝经期高血压病的临床观察[J].北京中医药大学学报,2012,35(9)：634-637.
[22]何佳,林雪,许红峰.虚燥更平散治疗更年期高血压阴虚内燥证神经内分泌免疫相关研究[J].时珍国医国药,2012,23(1)：0209-0211.
[23]马丽,周斌,杨娜,等.虚燥论治女性更年期高血压89例临床研究[J].新中医,2011,43(12)：20-22.
[24]贺玉琢.罗布麻叶提取物的降压作用[J].国外医学.中医中药分册.2005(03)：50-51.
[25]Hokis, Kimurs T, Nagasawa M, et al. Antihypommsive effects of the Principal tlavonoids of YAN LONG tea in spontaneously hypertensive rats[J]. Natural Medicines,2004,58(3):113-116.
[26]Tagawa c, Kagawa T, Nakazawa Y, et al. Studies on antihypertensive effect of Luobunm (Apoeynumvenetum L) leaf extract[J]. Yakugaku Zasshi,2004,124 (11):851-856.
[27]Kim DW, Yokcgaw T, Hattor M, et al. Effects of aqueous extracts of Apoeynum venetum leaves on Spotmmmusly hypermusive, renal hypertensive and NaCl-fed-hypertensive rats[J]. Ethnopharmacol,2000,72(1-2):53-59.
[28]Kagawa T, Nakazawa Y, Tngashirs E, et al. Studies on antihypertensive effect of luobuma (Apocynum venetum L) leaf extract[J]. Natural Medicines, 2004,58(3):109-112.
[29]洪秀芳,徐新娟,柏坤河,等.新疆大花罗布麻叶治疗高血压病临床观察实用 [J].中西医结合杂志,1991,4(1)：33.
[30]国家药典委员会.中国药典[M].北京：中国科技出版社,2010年版.
[31]梁明芝.桑椹的化学成分及药理作用[J].广西蚕业,2004,41(4)：39-41.
[32]黄勇,张林,赵卫国,等.桑椹的化学成分及药理作用研究进展[J].广西蚕业,2006,43(3)：15-19.
[33]高学敏.中药学[M].北京：中国中医药出版社,2002,16(6)：168-170.
[34]李兰青.地黄药理研究进展[J].中成药,1994,6(9)：47.
[35]冯建明,赵仁.三种地黄炮制品现代研究进展[J].云南中医学院学报,2000,23(4)：40-42.
[36]陈少刚,李长潮,庄学煊,等.当归注射液对家兔心肌缺血再灌注损伤的保护作用[J].中国中西医结合杂志,1995,15(8)：486-488.
[37]卢彦珍,赵艳峰,李宝红.当归对大鼠心肌梗死后早期左室重构的实验研究[J].长治医学院学报,2005,19(3)：164-167.
[38]王金宝,李恒芬,刘纪猛,等.酸枣仁汤对焦虑大鼠血浆神经肽Y的影响[J].四川中医,2009,27(6)：21.
[1]骆仲达,唐纯志,任容.针刺结合腹诊推拿对更年期综合征大鼠模型生殖-内分泌影响的研究[J].中国中医基础医学杂志,2010,16(5)：423-428.
[2]杨海燕,陈亚琼,辛晓燕.中药佳蓉片对去卵巢大鼠阴道细胞涂片的影响[J].第四军医大学学报,2001,22(6)：20.
[3]叶玉妹,杨慰,韩文均,等.宁神颗粒对实验性更年期雌性大鼠性激素和免疫功能的影响[J].时珍国医国药,2012,23(2)：344-345.
[4]崔慧慧,杨建一,李莉.不同类型高血压模型的研究进展[J].热带医学杂志,2008,8(5)：509-511.
[5]孙艳红,金若敏.女性更年期综合征动物模型制备的研究进展[J].时珍国医药,2005,6(7)：657-659.
[6]赖远征,冯冰虹,刘美珍等.补肾活血方对更年期大鼠性激素的影响[J].辽宁中医杂志,2004,31(1)：78-79.
[7]洪润肖.补糖干预对动情周期抑制大鼠HPT轴及HPA轴结构和功能的影响[D].上海体育学院,2011年,23-24.
[1]高振,阿地力江,哈木拉提·吾甫尔.西北寒燥证模型与IL-2、IL-6、INF-γ、TGF-α关系研究[J].时珍国医国药,2010,21(12)：3305-3307.
[2]樊蔚虹,岳广欣,李素香,等.长期激怒致肝肾阴虚证动物模型研制[J].中国中医基础医学杂志,2001(9)：67-69.
[3]高振,阿地力江·阿布力米提,胡汉华,等.西北寒燥证证候动物模型的生物学基础研究[J].科技导报,2009,27(2)：88-93.
[4]傅益群,吕健,动物模型与中医证候关系释义[J].中医药学刊,2004,22(9)：1666.
[5]何佳,李鹏,周铭心.西北燥证动物模型设想[J].时珍国医国药,2008,19(3)：529.
[6]哈木拉提·吾甫尔,阿依努尔·买提斯迪克,努尔买买提·艾买提,等.异常黑胆质证载体动物模型的建立及其自然恢复反证[J].新疆医科大学学报,2006,29(10)：910-914.
[7]高振,阿地力江·阿布力米提,哈木拉提·吾甫尔.干燥寒冷环境对小鼠生物表征的影响[J].科学导报,2008,26(14)：84-87.
[8]白平,傅晓晴,陈琪,等.男性暴力行为人群血中去甲肾上腺素等指标水平与中医辨证分型相关性探讨[J].中医杂志,2002,13(8)：620.
[9]沈自尹.我的科研历程和思考[J].中医杂志,2000,41(11)：690-692.
[10]杨翠平.温热药造小鼠阴虚模型的建立及其对小鼠抗氧化作用的影响[J].四川中医,2004,10(11)：15.
[11]王秋.家兔温病阴虚热盛证动物模型[J].辽宁中医杂志,1995,22(9)：427-429.
[12]汪泳涛,张伟荣,宋红普,等.长期超负荷游泳运动建立阴虚内热证动物模型的研究[J].中医药学刊,2003,21(7)：1115-1142.
[13]凌昌全,李敏,朱德增,等.阴阳虚证与糖皮质激素受体关系的临床与实验研究[J].浙江中西医结合杂志,2001,11(9)：532-533.
[14]夏洪生,张永锋,刘立昌,等.归蓉补血片对小鼠阴虚证模型作用的实验研究[J].湖北中医学院学报,2001,3(2)：23.
[15]张丽萍,杜彩霞.两地汤煎剂对拟更年期阴虚内热大鼠模型的影响[J].中医 研究,2007,2(8)：25-26.
[16]樊蔚虹.长期激怒法造模[J].中国中医基础医学杂志,2001,7(9)：68-69.
[17]任小巧,卢跃卿,邓伟,等.加味一贯煎对实验性肝肾阴虚证大鼠下丘脑-垂体-甲状腺轴的影响[J].中国中药志,2000,25(3)：172-174.
[18]方肇勤,潘志强,卢文丽,等.小鼠常见证候的辨证标准[J].中国中医基础医学杂志,2006,12(7)：508-510.
[19]刘文兰,张红月,穆阳,等.急性肝损伤肝肾阴虚证模型的建造和评价[J].辽宁中医药大学学报,2010,12(5)：41-43
[20]樊蔚虹,岳广欣,李素香,等.肝肾阴虚证大鼠下丘脑-垂体-甲状腺轴的变化及中药对其调节作用[J].中国中医药信息杂志,2001(10)：21-22.
[1]翟丽华.高血压血管重塑及药物干预后的逆向重塑[J].高血压杂志,2001(2)：76-80.
[2]李运伦,颜廷兰.清热降压方抗高血压血管重塑的实验研究[J].山东中医药大学学报,2005,29(2)：162-164.
[3]马雪玲,李玉波,陈建新.自发性高血压大鼠中医证侯及其理化指标相关性研究[J].世界中医药,2013,8(2)：134-137.
[4]刘涛,王伟.证候动物模型研究的思索[J].中华中医药杂志,2006,21(9)：550-551.
[5]尚玉红,林雪.更年期高血压中医研究进展[J].中国中医药杂志,2007,5(8)：42-44.
[6]姚一康.SHR大鼠病理特性测定研究[J].上海实验动物学,2003,23(2)：75-78.
[7]甘庆雷.虚燥更平散对更年期高血压大鼠血压及血压变异性影响的实验研究[D].新疆医科大学,2012年,26-27.
[8]Heagerty AM, Aalkjaer C, Bund SJ, et al. Small artery structure in hypertension. Dual proeesses of remodeling and growth[J]. Hypertension, 1993,21(4):391-397.
[9]Intengan HD, Schiffrin EL. Vaseular Remodeling in Hypertension roles of apoptosis[J]. Inflammation and Fibrosis Hypertension,2001,38:581-587.
[10]吴以岭.络病与血管病变的相关性研究及治疗[J].中医杂志,2006,47(3)：163-165.
[11]冀凯宏.自发性高血压大鼠主动脉结构重建[J].基础医学与临床,2000,20(4)：82-86.
[12]杨颖林.应用络病学论治高血压病浅识[J].实用中医内科杂志,2007,21(6)：61.
[13]张丽芳,柯元南.大鼠动脉内膜损伤后血管重塑相关因子的动态变化[J].中日友好医院学报,2007,21(2)：91-93.
[14]Li H, Simon H, Boean TM, et al. MMP/TIMP expression in spontaneously hypertensive heart failure rats:the effect of ACE and MMP-inhibition[J]. Cardiovase Res,2000,46(2):298-306.
[15]Yu Q, Stamenkovic I. Cell surface-localized matrix metalloproteinase-9 proteolytically activates TGF-β and promotes tumor invasion and angiogenesis[J]. Genes Dev,2000,14(2):163-176.
[16]袁丁.夏膝颗粒降压作用及对SHR心肌细胞外机制影响的实验研究[D].长春中医药大学,2009年,85-86.
[1]扈新刚,张允岭,柳洪胜.气虚血瘀证大鼠表征观察与分析[J].北京中医药大学学报,2006,29(12)：807-810.
[2]谭元生,王宇红.复方钩藤片对自发性高血压大鼠的降压作用及其机制[J].中国中医急症,2012,21(1)：52-54.
[3]杨新才,周瑞.三七花治疗高血压病的实验研究[J].天津中医药,2011,28(2)：145-147.
[4]甘庆雷.虚燥更平散对更年期高血压大鼠血压及血压变异性影响的实验研究[D].新疆医科大学,2012年,26-27.
[5]黄汉超,黄琳,吴永刚.六君丹参颗粒对自发性高血压大鼠心血管重塑干预作用的实验研究[J].中国中医急症,2012,21(1)：49-54.
[6]钟广伟,李炜,陈民敬.天麻、钩藤、石决明、牡蛎、牛膝混合剂对高血压大鼠血管重构的影响[J].中华高血压杂志,2008,16(9)：812-816.
[7]孟云辉,于慧卿,刘真.镇肝熄风汤对自发性高血压大鼠血管重塑的影响[J].河北中医,2010,32(7)：1065-1067.
[8]王博,张继东,冯进波,等.芩丹胶囊对自发性高血压大鼠血管重构以及骨桥蛋白基因表达的影响[J].南京中医药大学学报,2007,23(3)：157.
[9]李鸿霞.虚燥更平散对更年期高血压大鼠血管组织形态学及MMP-9、 TIMP-1影响的实验研究[D].新疆医科大学,2012年,26-30.
[1]张丽芳,柯元南.大鼠动脉内膜损伤后血管重塑相关因子的动态变化[J].中日友好医院学报,2007,21(2)：91-93.
[2]袁向珍.卡维地洛对大鼠颈动脉球囊损伤后再狭窄的影响和机制[D].南方医科大学,2007年,72-77.
[3]高璀乡,郭乃州,崔玉宝.冠心病患者基质金属蛋白酶-9和基质金属蛋白酶组织抑制物-1表达的临床意义[J].第四军医大学学报,2009,30(4)：335.
[4]徐贤雄,欧雅莉,杨天伦.氨氯地平与厄贝沙坦对原发性高血压病患者血清基质金属蛋白酶的影响[J].中国现代医学杂志,2010,20(14)：2201-2205.
[5]雷燕,陶丽丽,王国利.人参三七川芎提取物对增龄和高血压大鼠血管平滑肌细胞的影响[J].中国中西医结合杂志,2012,32(10)：1374-1379.
[6]孙敬和,冼绍祥,杨忠奇.天麻钩藤饮对SHR大鼠心肌胶原降解的影响[J].中药新药与临床药理,2010,21(6)：595-598.
[1]Bobik A. Transforming growth factor-betas and vascular disor-ders[J]. Arterioscler Thromb Vasc Biol,2006,26:1712-1720.
[2]Agrotis A, Saltis J, Bobik A, et al. Transforming growth factor-β1 gene activation and growth of smooth muscle from hypertensive rats[J]. Hypertension,1994,23(5):593-599.
[3]Alessandra C, Valeria B, Bruto R, et al. Vaseularendo the lial growth faetorinerease the migration and Proliferation of smooth musele cell sthrough the mediation of growth faetorsreleased by endothelialeells[J].Surg Res,2003,109:16.
[4]Suthanthiran M Li B,Song JO.etal. Ttransforming growth factor-β1 hyperer-expression in Africar-American hypertendsiver:a novel mediator if hypertension and/or target organ damage[J].Proc Natl Acad Sci,2000, 97(7):3479-3484.
[5]Piek E.Heldin CH.Ten Dijke P.et al.Specificity, diversity, and regulation in TGF-β, superfamily signaling [J]. FASEBJ,1999,13(15):2105-2124.
[6]王永霞,王世君,钱义明.三种治法对SHR血管AT1和TGF-β1表达影响的比较研究[J].辽宁中医杂志,2006,33(3)：376-378.
[7]单海燕,徐丹,胡秋艳.依那普利和非洛地平对原发性高血压患者TGF-β1、β2浓度的影响及临床意义的探讨[J].中国心血管杂志,2005,10(3)：204-206.
[2]Ezzati M, Lopez AD, Rodgers A, et al. Selected major risk factors and global and negional burden of disease[J]. Lancet,2002,360(9343):1347-1360.
[3]朱宝珍,范世慧.二仙汤加减对更年期妇女高血压患者生活质量的影响[J].河北中医,2007,29(1)：80-81.
[4]尚玉红,林雪.更年期高血压中医研究进展[J].中国中医药杂志,2007,5(8)：42-44.
[5]姚憬.更年期高血压之辨证施治[J].浙江中医杂志,2007,42(2)：110.
[6]周慧成.围绝经期动态血压变异性探讨[J].中国社区医师,2004,6(14)：67-68.
[7]魏相玲.更年期综合征的中医辨证治疗[J].光明中医,2008(6)：847-848.
[8]常惠,陈启光,闵婕.更年期综合征中医辨证现代化研究现场调研总结[J].浙江中医杂志,2004,(3)：100-103.
[9]李炳文,胡雪桔.浅谈女性更年期高血压病的防治[J].中医药临床杂志,2007,19(4)：336-337.
[10]高文军,杨国强.二仙汤加减治疗女性更年期高血压51例[J].陕西中医,2008,29(7)：784-785.
[11]刘平,崔卓敏.逍遥散联合西药治疗女性围绝经期原发性高血压59例[J].陕西中医,2009,30(7)：789-790.
[12]卢跃棣,叶开升.替米沙坦合更年安片治疗更年期高血压[J].浙江中西医结 合杂志,2007,17(2)：110-111.
[13]陶颖.中西医结合治疗绝经期女性合并高血压33例[J].中医药信息,2007,24(3)：52-53.
[14]叶盈,黄飞翔,王永等.六味地黄丸合络活喜治疗女性更年期高血压疗效观察[J].中国中医急症,2006,15(5)：487-488.
[15]李志,解发良.中西医结合并心理治疗女性高血压合并更年期综合征58例临床观察[J].中国中医药科技,2006,27(12)：137-138.
[16]周志昆,曾红兵,高植明.中药及复方调节神经内分泌免疫网络研究进展[J].湖南中医杂志,2006,22(2)：89.
[17]曾燕静,潘竞霞,刘柯兵等.温胆片治疗痰浊型高血压患者颈动脉粥样硬化的血流动力改变[J].内蒙古中医药,2008(2)：10.
[18]许娜,成丽.围绝经期定义[J].中国实用乡村医生杂志,2008,15(8)：1-3.
[19]古萍,王庆.血浆儿茶酚胺对血液透析患者高血压的影响[J].实用医学杂志,2004,20(3)：263-264.
[20]邱一华,彭聿平,王建军.儿茶酚胺调节免疫功能的细胞和分子机制[J].生理科学进展,2003,22(4)：16-21.
[21]汤健,唐朝枢,循环系统的内分泌功能[M].北京：北京医科大学、中国协和医科大学联合出版社,1989：121-124.
[22]袁公贤,哈黛文.多巴胺受体D1基因-48A/G多态性与原发性高血压病的关联研究[J].高血压杂志,2002(10)3：249-251.
[23]刘养凤,张军平等.冰片对大鼠血浆儿茶酚胺类物质影响的实验研究[J].天津中医药,2004,21(2)：58-59.
[24]Posnik-Urbanska A, Kawecka-jaszcz K. HyPertension in PostmenoPausal women-selected Pathome chanisms[J]. Przegl Lek,2006,63(12):1313-1317.
[25]郭雪微,王雪里红,高志凌.血浆神经肽Y神经降压肽水平与高血压患者血压昼夜变化和靶器官损害的研究[J].中华心血管病杂志,2005,33(11)：1006-1009.
[26]向菲,毛华.性激素与胰岛素抵抗关系在女性更年期高血压发病中的作用[J].贵州医药,2000,24(4)：237.
[27]Mendelsohn ME.Protective effects of estrogen on the cardiovascular system[J].Am J Cardiol,2002,89(supply):12E-18E.
[28]Chen Z, Yuhanna IS, Calcheva-Cargowa Z, et al. Eestogen receptor alpha mediates the non-genomic activation of endothelial nitric oxide synthase by estrogen[J]. Clin Invest,1999,325:756-762.
[29]张苏明,江红,何池中,等.更年期与阿尔茨海默病[J].国外医学,2001,28(2)：118.
[30]朱海燕,赖爱鸾,张李松.激素替代治疗联合降压药物对围绝经期原发性高血压妇女血压及内皮功能的影响[J].中国全科医学,2008,11(8)：1448-1450.
[1]崔慧慧,杨建一,李莉.不同类型高血压模型的研究进展[J].热带医学杂志,2008,58(5)：509-511.
[2]Liu K L, Lo M, Jean S. Lyon genetically hypertensive rats:an animal model of "low renin hypertension"[J]. Acta Pharmacologica Sinica,2003,24(1):1-6.
[3]范平,司军强,刘政江,等.自发性高血压大鼠模型的应用研究进展[J].农垦医学,2004,26(6)：444-448.
[4]孙艳红,金若敏.女性更年期综合征动物模型制备的研究进展[J].时珍国医药,2005,6(7)：657-659.
[5]赖远征,冯冰虹,刘美珍,等.补肾活血方对更年期大鼠性激素的影响[J].辽宁中医杂志,2004,31(1)：78-79.
[6]鲁遂荣,方学楹,侯安继,等.更年平调液对更年期大鼠自由基影响的实验研究[J].中国中医基础医学杂志,1998,4(8)：35-38.
[7]李仪奎.中药药理实验方法学(第二版)[M].上海：上海科学技术出版社,2006：831-839.
[8]李鸿霞.虚燥更平散对更年期高血压大鼠血管组织形态学及MMP-9、TIMP-1影响的实验研究[D].新疆医科大学,2012年,26-30.
[9]甘庆雷.虚燥更平散对更年期高血压大鼠血压及血压变异性影响的实验研究[D].新疆医科大学,2012年,26-27.
[10]陈小野.证候动物模型诊断依据的设想与评价[J].中国医药学报,1987,2(1)：50.
[11]周铭心.西北燥证研究概述[J].上海中医药杂志,2005,39(11)：43-45.
[12]白泊,高锡瑞,徐根才,等.中国地图集[M].北京：中国地图出版社,2006年版.
[13]沙塔娜提,孙红友,周铭心.环境地理因素与亚健康状态的关系初探-西北燥证背景研究[J].新疆中医药,2006,24(4)：74-75.
[14]Kelsey RM, Alpert BS, Patterson SM, et al. Radial differences in hemody namic responses to environmental thermal stress among ado escents[J]. Circulation,2000,01:2284-2289.
[15]吕光耀,李杰,周铭心.新疆不同地区不同民族居民生存质量与西北燥证关 系的分析[J].新疆医科大学学报,2007,30(1)：6-10.
[16]牟全胜,周铭心.西北多燥说[J].新疆中医药,1991,9(4)：1.
[17]高华,沙塔娜提·穆罕默德,周铭心.西北燥证与心理素质关系探讨[J].新疆医科大学学报,2007,30(1)：17-19.
[18]周铭心,宋晓平,单丽娟,等.新疆各地不同民族居民西北燥证罹患情况流行病学调查分析[J].新疆医科大学学报,2006,29(11)：1034-1038.
[19]傅益群,吕健.动物模型与中医证候关系释义[J].中医药学刊,2004,22(9)：1666.
[20]何佳,李鹏,周铭心.西北燥证动物模型设想[J].时珍国医国药,2008,19(3)：529.
[21]丁建中,张六通,邱幸凡.外燥之理论研究探析[J].中国中医基础医学杂志,2005,11(10)：724.
[22]哈木拉提·吾甫尔,阿依努尔·买提斯迪克,努尔买买提·艾买提,等.异常黑胆质证载体动物模型的建立及其自然恢复反证[J].新疆医科大学学报,2006,29(10)：910-914.
[23]高振,阿地力江·阿布力米提,哈木拉提·吾甫尔.干燥寒冷环境对小鼠生物表征的影响[J].科学导报,2008,26(14)：84-87.
[24]高振,阿地力江·阿布力米提,胡汉华.西北寒燥证证候动物模型的生物学基础研究[J].科技导报,2009,27(2)：88.
[25]高振,阿地力,哈木拉提·吾甫尔.西北寒燥证证候模型免疫功能紊乱的研究[J].中华中医药杂志,2010,25(8)：1225-1228.
[26]高振,阿地力江,哈木拉提·吾甫尔.西北寒燥证模型与IL-2、IL-6、INF-y、TNF-α关系研究[J].时珍国医国药,2010,21(12)：3305-3307.
[27]杨翠平.温热药造小鼠阴虚模型的建立及其对小鼠抗氧化作用的影响[J].四川中医,2004,10(11)：15.
[28]王秋.家兔温病阴虚热盛证动物模型[J].辽宁中医杂志,1995,22(9)：427-429.
[29]汪泳涛,张伟荣,宋红普,等.长期超负荷游泳运动建立阴虚内热证动物模型的研究[J].中医药学刊,2003,21(7)：1115-1142.
[30]凌昌全,李敏,朱德增,等.阴阳虚证与糖皮质激素受体关系的临床与实验研究[J].浙江中西医结合杂志,2001,11(9)：532-533.
[31]夏洪生,张永锋,刘立昌,等.归蓉补血片对小鼠阴虚证模型作用的实验研究[J].湖北中医学院学报,2001,3(2)：23.
[32]张丽萍,杜彩霞.两地汤煎剂对拟更年期阴虚内热大鼠模型的影响[J].中医研究,2007,2(8)：25-26.
[33]樊蔚虹.长期激怒法造模[J].中国中医基础医学杂志,2001,7(9)：68-69.
[34]任小巧,卢跃卿,邓伟,等.加味一贯煎对实验性肝肾阴虚证大鼠下丘脑-垂体-甲状腺轴的影响[J].中国中药志,2000,25(3)：172-174.
[35]方肇勤,潘志强,卢文丽,等.小鼠常见证候的辨证标准[J].中国中医基础医学杂志,2006,12(7)：508-510.
[36]郑春叶,黄燕.中医证候动物模型复制管见[J].中华中医药学刊,2007,25(5)：914.
[37]龚婕宁,杨进.家兔病毒性肺热证模型的建立[J].中国中医基础医学杂志,1995,1(3)：46-48.
[38]彭成.脾气虚脾不统血证候动物模型的研究思路[J].中医杂志,1996,37(4)：241.
[1]朱宝珍,范世慧.二仙汤加减对更年期妇女高血压患者生活质量的影响[J].河北中医,2007,29(1)：80-81.
[2]翟丽华.高血压血管重塑及药物干预后的逆向重塑[J].高血压杂志,2001(2)：76-80.
[3]李鸿霞.虚燥更平散对更年期高血压大鼠血管组织形态学及MMP-9、 TIMP-1影响的实验研究[D].新疆医科大学,2012年,26-30.
[4]甘庆雷.虚燥更平散对更年期高血压大鼠血压及血压变异性影响的实验研究[D].新疆医科大学,2012年,26-27.
[5]Hope D, Intenganrnestol, Schiffrin. Vascular Remodeling in Hypertension: Role of Apoptosis, Inflammation and Fibrosis[J]. Hypertension,2001,38: 581-587.
[6]Bussy C, Bouyrie P, Lacolley P, et al. Intrinsic stiffs soft hearted arterial wall arterial inessential hypertensives[J]. Hypertension,2000,35(5):1049-1054.
[7]Dumonto, Loufrani L, Henrion D. Keyrole of the Npathway and matrix metal Protease-9 in high blood flow-induced remodeling of rat resorted near tries[J]. Arteriosler Thromb Vase Biol,2007,27(2):317-324.
[8]徐鹏宵,李红,孙昭英.自发性高血压大鼠心肌血管内皮生长因子表达水平的检测[J].武警医学,2005,6(7)：491-493.
[9]Bobik A. Transforming growth factor betas and vascular disorders[J]. Arterioscler Thromb Vasc Biol,2006,26:1712-1720.
[10]Park J, Ha H, Ahn HJ, et al. Sirolimus inhibits platelet-derived growth factor-induced collagen synthesis in rat vascular smooth muscle cells[J]. Transplant Proc,2005,37:3459-3462.
[11]胡锦生,乔木.内皮功能与心血管疾病[J].岭南心血管病杂 志,2000,6：327-331.
[12]Kenneth MY. Fibroin acting peptides in cell migration and wound repair[J]. Clini Invest,2000,105(11):1507-1509.
[13]李庆平,陆泽安,绕曼人.粉防己碱抑制VSMC胶原合成[J].药学学报,2001,36(7)：481-484.
[14]白江涛.基质金属蛋白酶与心血管疾病[J].心血管病学进展,2003,24(1)：43-47.
[15]Intengan HD, Schiffrin EL. Vaseular Remodeling in Hypertension. Roles of Apoptosis, Inflammation and Fibrosis[J]. Hypertension,2001,38:581-587.
[16]Ergul A, Portik-Dobos V, Hutchinson J, et al. Down regulation of vascular matrix metalloproteinase inducer and activator proteins in hypertensive patients[J]. Am J Hypertens,2004,17:775-782.
[17]张丽芳,柯元南.大鼠动脉内膜损伤后血管重塑相关因子的动态变化[J].中日友好医院学报,2007,21：91-93.
[18]Peterson JT, Li H, Dillon L, et al. Evolution of matrix metal proteins and tissue inhibitor expression during heart failure progression in the infracted rat[J]. Cardio vase Res,2000,46(2):307-315.
[19]Li H, Simon H, Bocan TM, et al. MMP/TIMP expression in spontaneously hypertensive heart failure rats:the effect of ACE-and MMP-inhibition[J]. Cardio vase Res,2000,46:298-306.
[20]Begona Lopez, Arantxa Gonzalez,Ram6n Querejeta, et al. Alterations in Pattern of Collagen Deposition May Contribute to the Deterioration of Systolic Function in Hypertensive Patienis with Heart Failure[J]. Am Coll Cardiol,2006,89-96.
[21]徐贤雄,欧雅莉,杨天伦.氨氯地平与厄贝沙坦对原发性高血压患者血清基质金属蛋白酶的影响[J].中国现代医学杂志,2010,20(14)：2201-2203.
[22]周虹,邢之华,刘卫平,等.动态血压分析天麻钩藤饮的降压效果[J].湖南中医学院学报,2005,25(4)：40-41.
[23]曾燕静,潘竞霞,刘柯兵,等.温胆片治疗痰浊型高血压患者颈动脉粥样硬化的血流动力改变[J].内蒙古中医药,2008(2)：10.
[24]王华军,谢良地,姚恩辉,等.通心络对自发性高血压大鼠阻力血管结构的影响[J].高血压杂志,2006,14(1)：61-63.
[25]史载祥.后再灌注时代难题的中西医结合治疗思考[J].中西医结合心脑血管病杂志,2005,3(1)：2-5.
[26]徐凤芹,徐浩,刘剑刚,等.芎芍胶囊对动脉粥样硬化兔血管平滑肌细胞增殖的影响[J].中国中西医结合杂志,2008,28(10)：912.
[27]徐凤芹,徐浩,马鲁波,等.芎芍胶囊对动脉粥样硬化兔血管平滑肌细胞凋亡的影响[J].中西医结合心脑血管病杂志,2008,6(7)：794.
[28]余胜,董建勋,郝钰,等.舒脉胶囊对Ang Ⅱ诱导的血管平滑肌细胞增殖及细胞周期的影响[J].北京中医药大学学报,2009,32(3)：178.
[29]翟丽华.高血压血管重塑及药物干预后的逆向重塑[J].高血压杂志,2001(2)：76-80.
[30]车在前,顾天华,吴永杰,等.苯丙氨酸缓解高血压大鼠血管重塑[J].中国病理生理杂志,2005,21(2)：327-330.
[1]周春绵,王陆建.血压变异性的机制和临床意义[J].临床荟萃,2003,18(11)：657-658.
[2]沙塔娜提,孙红友,周铭心.环境地理因素与亚健康状态的关系初探-西北燥证背景研究[J].新疆中医药,2006,24(4)：74-75.
[3]周铭心.西北燥证研究概述[J].上海中医药杂志,2005,39(11)：43-45.
[4]吕光耀,李杰,周铭心.新疆不同地区不同民族居民生存质量与西北燥证关系的分析[J].新疆医科大学学报,2007,30(1)：6-10.
[5]林雪,尚玉红,马丽,等.环境地理因素与更年期高血压的关系探微[J].中华中医药,2008,23(2)：125-127.
[6]林雪,余承云,雷震云,等.女性更年期高血压阴虚内燥证和非阴虚内燥证与性激素及血脂血糖水平的相关性研究[J].中国中西医结合杂志,2009,29(7)：603-606.
[7]吕光耀,李杰,周铭心.燥证及西北燥证病因病机研究概述[J].新疆医科大学学报,2007,30(1)：26-28.
[8]李南方,周玲,曹梅,等.新疆和丰县牧民高血压病流行病学调查报告[J].现代预防医学,2003,30(2)：141-143.
[9]林雪,雷震云,余承云,等.虚燥更平颗粒对女性更年期高血压血压变异性的影响[J].新疆医科大学学报,2008,31(12)：1753-1756.
[10]林雪,尚玉红,杨娜.刘继祖治疗更年期女性高血压病经验[J].新疆中医药,2006,24(2)：37-38.
[11]杨晓钊.中西医结合治疗妇女更年期高血压60例疗效观察[J].广西医科大学报,2001,18(4)：561-562.
[12]罗元凯.实用中医妇科学[M].上海：上海科学技术出版社,1994,113.
[13]雷燕.络病理论探微[J].北京中医药大学学报,1998,21(2)：18-23.
[14]杨传华,陆峰,张翠英.高血压血管重塑与络病的相关性[J].山东中医杂志,2005,11(24)：643-646.
[15]吴以岭.络病与血管病变的相关性研究及治疗[J].中医杂志,2006,47(3)：163-165.
[16]安乐君,葛丽,林雪.黄永生治疗更年期高血压病经验探微[J].中国实验方剂学杂志,2012,24(18)：360-362.
[17]秦长林.燥证琐谈[J].湖北中医杂志,2001,23(8)：6-7.
[18]王芳,张亚宏,左代英,等.红人归胶囊治疗更年期综合征作用机制初探[J].沈阳药科大学学报,2008,25(9)：739-742.
[19]马丽,林雪.虚燥更平颗粒对阴虚内燥证女性高血压病(围绝经期)患者生活质量的影响[J].中华中医药杂志,2012,27(3)：547-551.
[20]林雪,马丽,李红霞.虚燥更平散治疗围绝经期高血压病的临床观察[J].中华中医药杂志,2012,27(11)：2986-2988.
[21]何佳,迟新栋,林雪.虚燥更平散治疗围绝经期高血压病的临床观察[J].北京中医药大学学报,2012,35(9)：634-637.
[22]何佳,林雪,许红峰.虚燥更平散治疗更年期高血压阴虚内燥证神经内分泌免疫相关研究[J].时珍国医国药,2012,23(1)：0209-0211.
[23]马丽,周斌,杨娜,等.虚燥论治女性更年期高血压89例临床研究[J].新中医,2011,43(12)：20-22.
[24]贺玉琢.罗布麻叶提取物的降压作用[J].国外医学.中医中药分册.2005(03)：50-51.
[25]Hokis, Kimurs T, Nagasawa M, et al. Antihypommsive effects of the Principal tlavonoids of YAN LONG tea in spontaneously hypertensive rats[J]. Natural Medicines,2004,58(3):113-116.
[26]Tagawa c, Kagawa T, Nakazawa Y, et al. Studies on antihypertensive effect of Luobunm (Apoeynumvenetum L) leaf extract[J]. Yakugaku Zasshi,2004,124 (11):851-856.
[27]Kim DW, Yokcgaw T, Hattor M, et al. Effects of aqueous extracts of Apoeynum venetum leaves on Spotmmmusly hypermusive, renal hypertensive and NaCl-fed-hypertensive rats[J]. Ethnopharmacol,2000,72(1-2):53-59.
[28]Kagawa T, Nakazawa Y, Tngashirs E, et al. Studies on antihypertensive effect of luobuma (Apocynum venetum L) leaf extract[J]. Natural Medicines, 2004,58(3):109-112.
[29]洪秀芳,徐新娟,柏坤河,等.新疆大花罗布麻叶治疗高血压病临床观察实用 [J].中西医结合杂志,1991,4(1)：33.
[30]国家药典委员会.中国药典[M].北京：中国科技出版社,2010年版.
[31]梁明芝.桑椹的化学成分及药理作用[J].广西蚕业,2004,41(4)：39-41.
[32]黄勇,张林,赵卫国,等.桑椹的化学成分及药理作用研究进展[J].广西蚕业,2006,43(3)：15-19.
[33]高学敏.中药学[M].北京：中国中医药出版社,2002,16(6)：168-170.
[34]李兰青.地黄药理研究进展[J].中成药,1994,6(9)：47.
[35]冯建明,赵仁.三种地黄炮制品现代研究进展[J].云南中医学院学报,2000,23(4)：40-42.
[36]陈少刚,李长潮,庄学煊,等.当归注射液对家兔心肌缺血再灌注损伤的保护作用[J].中国中西医结合杂志,1995,15(8)：486-488.
[37]卢彦珍,赵艳峰,李宝红.当归对大鼠心肌梗死后早期左室重构的实验研究[J].长治医学院学报,2005,19(3)：164-167.
[38]王金宝,李恒芬,刘纪猛,等.酸枣仁汤对焦虑大鼠血浆神经肽Y的影响[J].四川中医,2009,27(6)：21.
[1]骆仲达,唐纯志,任容.针刺结合腹诊推拿对更年期综合征大鼠模型生殖-内分泌影响的研究[J].中国中医基础医学杂志,2010,16(5)：423-428.
[2]杨海燕,陈亚琼,辛晓燕.中药佳蓉片对去卵巢大鼠阴道细胞涂片的影响[J].第四军医大学学报,2001,22(6)：20.
[3]叶玉妹,杨慰,韩文均,等.宁神颗粒对实验性更年期雌性大鼠性激素和免疫功能的影响[J].时珍国医国药,2012,23(2)：344-345.
[4]崔慧慧,杨建一,李莉.不同类型高血压模型的研究进展[J].热带医学杂志,2008,8(5)：509-511.
[5]孙艳红,金若敏.女性更年期综合征动物模型制备的研究进展[J].时珍国医药,2005,6(7)：657-659.
[6]赖远征,冯冰虹,刘美珍等.补肾活血方对更年期大鼠性激素的影响[J].辽宁中医杂志,2004,31(1)：78-79.
[7]洪润肖.补糖干预对动情周期抑制大鼠HPT轴及HPA轴结构和功能的影响[D].上海体育学院,2011年,23-24.
[1]高振,阿地力江,哈木拉提·吾甫尔.西北寒燥证模型与IL-2、IL-6、INF-γ、TGF-α关系研究[J].时珍国医国药,2010,21(12)：3305-3307.
[2]樊蔚虹,岳广欣,李素香,等.长期激怒致肝肾阴虚证动物模型研制[J].中国中医基础医学杂志,2001(9)：67-69.
[3]高振,阿地力江·阿布力米提,胡汉华,等.西北寒燥证证候动物模型的生物学基础研究[J].科技导报,2009,27(2)：88-93.
[4]傅益群,吕健,动物模型与中医证候关系释义[J].中医药学刊,2004,22(9)：1666.
[5]何佳,李鹏,周铭心.西北燥证动物模型设想[J].时珍国医国药,2008,19(3)：529.
[6]哈木拉提·吾甫尔,阿依努尔·买提斯迪克,努尔买买提·艾买提,等.异常黑胆质证载体动物模型的建立及其自然恢复反证[J].新疆医科大学学报,2006,29(10)：910-914.
[7]高振,阿地力江·阿布力米提,哈木拉提·吾甫尔.干燥寒冷环境对小鼠生物表征的影响[J].科学导报,2008,26(14)：84-87.
[8]白平,傅晓晴,陈琪,等.男性暴力行为人群血中去甲肾上腺素等指标水平与中医辨证分型相关性探讨[J].中医杂志,2002,13(8)：620.
[9]沈自尹.我的科研历程和思考[J].中医杂志,2000,41(11)：690-692.
[10]杨翠平.温热药造小鼠阴虚模型的建立及其对小鼠抗氧化作用的影响[J].四川中医,2004,10(11)：15.
[11]王秋.家兔温病阴虚热盛证动物模型[J].辽宁中医杂志,1995,22(9)：427-429.
[12]汪泳涛,张伟荣,宋红普,等.长期超负荷游泳运动建立阴虚内热证动物模型的研究[J].中医药学刊,2003,21(7)：1115-1142.
[13]凌昌全,李敏,朱德增,等.阴阳虚证与糖皮质激素受体关系的临床与实验研究[J].浙江中西医结合杂志,2001,11(9)：532-533.
[14]夏洪生,张永锋,刘立昌,等.归蓉补血片对小鼠阴虚证模型作用的实验研究[J].湖北中医学院学报,2001,3(2)：23.
[15]张丽萍,杜彩霞.两地汤煎剂对拟更年期阴虚内热大鼠模型的影响[J].中医 研究,2007,2(8)：25-26.
[16]樊蔚虹.长期激怒法造模[J].中国中医基础医学杂志,2001,7(9)：68-69.
[17]任小巧,卢跃卿,邓伟,等.加味一贯煎对实验性肝肾阴虚证大鼠下丘脑-垂体-甲状腺轴的影响[J].中国中药志,2000,25(3)：172-174.
[18]方肇勤,潘志强,卢文丽,等.小鼠常见证候的辨证标准[J].中国中医基础医学杂志,2006,12(7)：508-510.
[19]刘文兰,张红月,穆阳,等.急性肝损伤肝肾阴虚证模型的建造和评价[J].辽宁中医药大学学报,2010,12(5)：41-43
[20]樊蔚虹,岳广欣,李素香,等.肝肾阴虚证大鼠下丘脑-垂体-甲状腺轴的变化及中药对其调节作用[J].中国中医药信息杂志,2001(10)：21-22.
[1]翟丽华.高血压血管重塑及药物干预后的逆向重塑[J].高血压杂志,2001(2)：76-80.
[2]李运伦,颜廷兰.清热降压方抗高血压血管重塑的实验研究[J].山东中医药大学学报,2005,29(2)：162-164.
[3]马雪玲,李玉波,陈建新.自发性高血压大鼠中医证侯及其理化指标相关性研究[J].世界中医药,2013,8(2)：134-137.
[4]刘涛,王伟.证候动物模型研究的思索[J].中华中医药杂志,2006,21(9)：550-551.
[5]尚玉红,林雪.更年期高血压中医研究进展[J].中国中医药杂志,2007,5(8)：42-44.
[6]姚一康.SHR大鼠病理特性测定研究[J].上海实验动物学,2003,23(2)：75-78.
[7]甘庆雷.虚燥更平散对更年期高血压大鼠血压及血压变异性影响的实验研究[D].新疆医科大学,2012年,26-27.
[8]Heagerty AM, Aalkjaer C, Bund SJ, et al. Small artery structure in hypertension. Dual proeesses of remodeling and growth[J]. Hypertension, 1993,21(4):391-397.
[9]Intengan HD, Schiffrin EL. Vaseular Remodeling in Hypertension roles of apoptosis[J]. Inflammation and Fibrosis Hypertension,2001,38:581-587.
[10]吴以岭.络病与血管病变的相关性研究及治疗[J].中医杂志,2006,47(3)：163-165.
[11]冀凯宏.自发性高血压大鼠主动脉结构重建[J].基础医学与临床,2000,20(4)：82-86.
[12]杨颖林.应用络病学论治高血压病浅识[J].实用中医内科杂志,2007,21(6)：61.
[13]张丽芳,柯元南.大鼠动脉内膜损伤后血管重塑相关因子的动态变化[J].中日友好医院学报,2007,21(2)：91-93.
[14]Li H, Simon H, Boean TM, et al. MMP/TIMP expression in spontaneously hypertensive heart failure rats:the effect of ACE and MMP-inhibition[J]. Cardiovase Res,2000,46(2):298-306.
[15]Yu Q, Stamenkovic I. Cell surface-localized matrix metalloproteinase-9 proteolytically activates TGF-β and promotes tumor invasion and angiogenesis[J]. Genes Dev,2000,14(2):163-176.
[16]袁丁.夏膝颗粒降压作用及对SHR心肌细胞外机制影响的实验研究[D].长春中医药大学,2009年,85-86.
[1]扈新刚,张允岭,柳洪胜.气虚血瘀证大鼠表征观察与分析[J].北京中医药大学学报,2006,29(12)：807-810.
[2]谭元生,王宇红.复方钩藤片对自发性高血压大鼠的降压作用及其机制[J].中国中医急症,2012,21(1)：52-54.
[3]杨新才,周瑞.三七花治疗高血压病的实验研究[J].天津中医药,2011,28(2)：145-147.
[4]甘庆雷.虚燥更平散对更年期高血压大鼠血压及血压变异性影响的实验研究[D].新疆医科大学,2012年,26-27.
[5]黄汉超,黄琳,吴永刚.六君丹参颗粒对自发性高血压大鼠心血管重塑干预作用的实验研究[J].中国中医急症,2012,21(1)：49-54.
[6]钟广伟,李炜,陈民敬.天麻、钩藤、石决明、牡蛎、牛膝混合剂对高血压大鼠血管重构的影响[J].中华高血压杂志,2008,16(9)：812-816.
[7]孟云辉,于慧卿,刘真.镇肝熄风汤对自发性高血压大鼠血管重塑的影响[J].河北中医,2010,32(7)：1065-1067.
[8]王博,张继东,冯进波,等.芩丹胶囊对自发性高血压大鼠血管重构以及骨桥蛋白基因表达的影响[J].南京中医药大学学报,2007,23(3)：157.
[9]李鸿霞.虚燥更平散对更年期高血压大鼠血管组织形态学及MMP-9、 TIMP-1影响的实验研究[D].新疆医科大学,2012年,26-30.
[1]张丽芳,柯元南.大鼠动脉内膜损伤后血管重塑相关因子的动态变化[J].中日友好医院学报,2007,21(2)：91-93.
[2]袁向珍.卡维地洛对大鼠颈动脉球囊损伤后再狭窄的影响和机制[D].南方医科大学,2007年,72-77.
[3]高璀乡,郭乃州,崔玉宝.冠心病患者基质金属蛋白酶-9和基质金属蛋白酶组织抑制物-1表达的临床意义[J].第四军医大学学报,2009,30(4)：335.
[4]徐贤雄,欧雅莉,杨天伦.氨氯地平与厄贝沙坦对原发性高血压病患者血清基质金属蛋白酶的影响[J].中国现代医学杂志,2010,20(14)：2201-2205.
[5]雷燕,陶丽丽,王国利.人参三七川芎提取物对增龄和高血压大鼠血管平滑肌细胞的影响[J].中国中西医结合杂志,2012,32(10)：1374-1379.
[6]孙敬和,冼绍祥,杨忠奇.天麻钩藤饮对SHR大鼠心肌胶原降解的影响[J].中药新药与临床药理,2010,21(6)：595-598.
[1]Bobik A. Transforming growth factor-betas and vascular disor-ders[J]. Arterioscler Thromb Vasc Biol,2006,26:1712-1720.
[2]Agrotis A, Saltis J, Bobik A, et al. Transforming growth factor-β1 gene activation and growth of smooth muscle from hypertensive rats[J]. Hypertension,1994,23(5):593-599.
[3]Alessandra C, Valeria B, Bruto R, et al. Vaseularendo the lial growth faetorinerease the migration and Proliferation of smooth musele cell sthrough the mediation of growth faetorsreleased by endothelialeells[J].Surg Res,2003,109:16.
[4]Suthanthiran M Li B,Song JO.etal. Ttransforming growth factor-β1 hyperer-expression in Africar-American hypertendsiver:a novel mediator if hypertension and/or target organ damage[J].Proc Natl Acad Sci,2000, 97(7):3479-3484.
[5]Piek E.Heldin CH.Ten Dijke P.et al.Specificity, diversity, and regulation in TGF-β, superfamily signaling [J]. FASEBJ,1999,13(15):2105-2124.
[6]王永霞,王世君,钱义明.三种治法对SHR血管AT1和TGF-β1表达影响的比较研究[J].辽宁中医杂志,2006,33(3)：376-378.
[7]单海燕,徐丹,胡秋艳.依那普利和非洛地平对原发性高血压患者TGF-β1、β2浓度的影响及临床意义的探讨[J].中国心血管杂志,2005,10(3)：204-206.
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