β-榄香烯对膀胱癌T24细胞增殖及MTA-1基因表达的影响
摘要
目的:研究β-榄香烯对膀胱癌T24细胞增殖及其对MTA-1基因表达的影响,探讨β-榄香烯诱导膀胱癌细胞凋亡及其可能机制,为进一步研究β-榄香烯的抗癌作用和临床应用β-榄香烯提供实验依据。
方法:体外培养T24细胞,以浓度分别为0.004mg/ml、0.008mg/ml、0.01mg/ml、0.02mg/ml、0.04mg/ml、0.06mg/ml、0.08mg/ml的β-榄香烯分别作用T24细胞24h、48h和72h,应用MTT方法测定各组β-榄香烯对膀胱癌T24细胞增殖的抑制作用;运用IC50软件计算出高于IC50所用的最低浓度β-榄香烯,以此浓度的β-榄香烯作用T24细胞24h、48h、72h,分别运用Western-blot、RT-PCR法检测对MTA-1蛋白和MTA-1mRNA表达水平的影响;应用光镜观察各组膀胱癌细胞形态学的改变。并设置浓度为0mg/mlβ-榄香烯作为对照组。
结果: MTT比色法结果显示:对T24细胞的抑制率在50﹪以上的最低浓度β-榄香烯为0.02mg/ml;其它各处理组不同浓度的β-榄香烯均能抑制T24细胞生长,并且T24细胞的成活率随β-榄香烯的浓度增加和作用时间的延长而降低,存在剂量和作用时间依赖性。以0.02mg/ml浓度β-榄香烯作用T24细胞24h、48h和72h, Western-blot检测各组MTA-1蛋白表达相对值分别是(0.845±0.014),(0.667±0.016),(0.478±0.018),与对照组(0.975±0.016)比较差别有显著性(P<0.05),而各处理组组间比较差别有显著性(P<0.01);RT-PCR检测各组MTA-1mRNA表达相对值分别是(0.867±0.005),(0.797±0.004),(0.717±0.007),与对照组(0.992±0.009)比较差别有显著性(P<0.05),而各处理组组间比较差别有显著性(P<0.01),这些结果显示β-榄香烯能够降低T24细胞中MTA-1蛋白和MTA-1mRNA的表达,并呈时间依赖性。以0.02mg/ml浓度β-榄香烯作用T24细胞24h、48h和72h,光镜观察各处理组T24细胞形态结果显示:随着0.02mg/ml浓度β-榄香烯作用时间的延长,细胞形状逐渐变为圆形或不规则形,数量增多,贴壁能力下降,表面出现发芽现象,细胞体积明显缩小,胞浆浓缩,胞核比例明显变小,出现单层细胞漂浮、掀起,部分细胞崩解成碎片,或失去了细胞正常形态。
结论:β-榄香烯能明显抑制膀胱癌T24细胞的生长和增殖,可诱导膀胱癌T24细胞凋亡。其机制可能与下调膀胱癌T24细胞MTA-1蛋白和MTA-1mRNA的表达有关。
Objective: To study theβ-elemene on cell proliferation in T24 bladder tumor cell lines and its expression of MTA-1, deeply investigated how theβ-elemene induced apoptosis of bladder cancer and its possible mechanism .In order to provide experimental evidence for the further study ofβ-elemene anti-cancer action and clinical application.
Methods: T24 cell lines which were cultured in vitro, were affected by theβ-elemene0.004mg/ml,0.008mg/ml,0.01mg/ml,0.02mg/ml,0.04mg/ml,0.06mg/ml,0.08mg/ml for 24 hours, 48 hours and 72 hours later, The samples were detected by the MTT-method so as to show the effect ofβ-elemene on proliferation of bladder cancer T24 cell lines; and then used the software to calculate the consistence higher than the minimum IC50. Use the Western-blot , RT-PCR assay and cell morphology to reveal the influence on the expression of MTA-1 and the level of mRNA ,and also showd bladder cancer cells morphological changes. The comparative group(ie.0mg/ml) was set up for comparation.
Results: MTT assay results showed that the minimumβ-elemene concentration which could inhibit more than 50% T24 cell lines was 0.02mg/ml; while the other treatment groups of drugs can inhibit the growth of T24 cell lines and the survival rate of T24 cell lines were reduced with the concentrations increased or the time extended. It means that dependence of dose and time existed. The relative density of MTA-1 in T24 which were affected underβ-elemene (Control group, 0.02mg/ml 24 hours, 48 hours and 72 hours group) were (0.975±0.016), (0.845±0.014), (0.667±0.016), (0.478±0.018), with the difference being statistically significant (p<0.01); the relative density of MTA-1 mRNA in T24 which were affected underβ-elemene (Control group, 0.02mg/ml 24 hours, 48 hours and 72 hours group) were (0.992±0.009), (0.867±0.005),(0.797±0.004),(0.717±0.007), with the difference being statistically significant (p<0.01),these results revealed thatβ-elemene could decrease MTA-1 in T24 cell lines and The expression of mRNA and time-dependent manner.T24 cell lines morphology showed that 0.02mg / ml concentration ofβ-elemene(24 hours, 48 hours and 72 hours group), cell shape gradually become round or irregular,the number increased adherence capacity decreased, the surface of sprouting, cell volume was significantly reduced, plasma concentration, the nucleus was significantly smaller proportion appeared floating single cells and caused some cell disintegration into pieces, or loss of normal cells form.
Conclusion:β-elemene could inhibit the T24 bladder cancer cell lines growth and proliferation, which may decrease the T24 cell lines with MTA-1 and MTA-1 mRNA expression.
方法:体外培养T24细胞,以浓度分别为0.004mg/ml、0.008mg/ml、0.01mg/ml、0.02mg/ml、0.04mg/ml、0.06mg/ml、0.08mg/ml的β-榄香烯分别作用T24细胞24h、48h和72h,应用MTT方法测定各组β-榄香烯对膀胱癌T24细胞增殖的抑制作用;运用IC50软件计算出高于IC50所用的最低浓度β-榄香烯,以此浓度的β-榄香烯作用T24细胞24h、48h、72h,分别运用Western-blot、RT-PCR法检测对MTA-1蛋白和MTA-1mRNA表达水平的影响;应用光镜观察各组膀胱癌细胞形态学的改变。并设置浓度为0mg/mlβ-榄香烯作为对照组。
结果: MTT比色法结果显示:对T24细胞的抑制率在50﹪以上的最低浓度β-榄香烯为0.02mg/ml;其它各处理组不同浓度的β-榄香烯均能抑制T24细胞生长,并且T24细胞的成活率随β-榄香烯的浓度增加和作用时间的延长而降低,存在剂量和作用时间依赖性。以0.02mg/ml浓度β-榄香烯作用T24细胞24h、48h和72h, Western-blot检测各组MTA-1蛋白表达相对值分别是(0.845±0.014),(0.667±0.016),(0.478±0.018),与对照组(0.975±0.016)比较差别有显著性(P<0.05),而各处理组组间比较差别有显著性(P<0.01);RT-PCR检测各组MTA-1mRNA表达相对值分别是(0.867±0.005),(0.797±0.004),(0.717±0.007),与对照组(0.992±0.009)比较差别有显著性(P<0.05),而各处理组组间比较差别有显著性(P<0.01),这些结果显示β-榄香烯能够降低T24细胞中MTA-1蛋白和MTA-1mRNA的表达,并呈时间依赖性。以0.02mg/ml浓度β-榄香烯作用T24细胞24h、48h和72h,光镜观察各处理组T24细胞形态结果显示:随着0.02mg/ml浓度β-榄香烯作用时间的延长,细胞形状逐渐变为圆形或不规则形,数量增多,贴壁能力下降,表面出现发芽现象,细胞体积明显缩小,胞浆浓缩,胞核比例明显变小,出现单层细胞漂浮、掀起,部分细胞崩解成碎片,或失去了细胞正常形态。
结论:β-榄香烯能明显抑制膀胱癌T24细胞的生长和增殖,可诱导膀胱癌T24细胞凋亡。其机制可能与下调膀胱癌T24细胞MTA-1蛋白和MTA-1mRNA的表达有关。
Objective: To study theβ-elemene on cell proliferation in T24 bladder tumor cell lines and its expression of MTA-1, deeply investigated how theβ-elemene induced apoptosis of bladder cancer and its possible mechanism .In order to provide experimental evidence for the further study ofβ-elemene anti-cancer action and clinical application.
Methods: T24 cell lines which were cultured in vitro, were affected by theβ-elemene0.004mg/ml,0.008mg/ml,0.01mg/ml,0.02mg/ml,0.04mg/ml,0.06mg/ml,0.08mg/ml for 24 hours, 48 hours and 72 hours later, The samples were detected by the MTT-method so as to show the effect ofβ-elemene on proliferation of bladder cancer T24 cell lines; and then used the software to calculate the consistence higher than the minimum IC50. Use the Western-blot , RT-PCR assay and cell morphology to reveal the influence on the expression of MTA-1 and the level of mRNA ,and also showd bladder cancer cells morphological changes. The comparative group(ie.0mg/ml) was set up for comparation.
Results: MTT assay results showed that the minimumβ-elemene concentration which could inhibit more than 50% T24 cell lines was 0.02mg/ml; while the other treatment groups of drugs can inhibit the growth of T24 cell lines and the survival rate of T24 cell lines were reduced with the concentrations increased or the time extended. It means that dependence of dose and time existed. The relative density of MTA-1 in T24 which were affected underβ-elemene (Control group, 0.02mg/ml 24 hours, 48 hours and 72 hours group) were (0.975±0.016), (0.845±0.014), (0.667±0.016), (0.478±0.018), with the difference being statistically significant (p<0.01); the relative density of MTA-1 mRNA in T24 which were affected underβ-elemene (Control group, 0.02mg/ml 24 hours, 48 hours and 72 hours group) were (0.992±0.009), (0.867±0.005),(0.797±0.004),(0.717±0.007), with the difference being statistically significant (p<0.01),these results revealed thatβ-elemene could decrease MTA-1 in T24 cell lines and The expression of mRNA and time-dependent manner.T24 cell lines morphology showed that 0.02mg / ml concentration ofβ-elemene(24 hours, 48 hours and 72 hours group), cell shape gradually become round or irregular,the number increased adherence capacity decreased, the surface of sprouting, cell volume was significantly reduced, plasma concentration, the nucleus was significantly smaller proportion appeared floating single cells and caused some cell disintegration into pieces, or loss of normal cells form.
Conclusion:β-elemene could inhibit the T24 bladder cancer cell lines growth and proliferation, which may decrease the T24 cell lines with MTA-1 and MTA-1 mRNA expression.
引文
1汤秀红,秦叔逵,谢恬,等.榄香烯注射液抗肿瘤作用基础研究的现状和进展[J].临床肿瘤学杂志,2010,166(5):266-272
2曹丽萍,沈洪,刘丽,等.黄芪甲苷、β-榄香烯对SGC7901胃癌细胞COX-及PGE-2表达的影响[J].现代中西医结合杂志,2010,19(7):68-70
3 Kumar R,Wang RA, Bagheri YR. Emerging roles of MTA1 family members in human cancers [J]. Semin Oncol,2003,30(5):3037
4魏新设,陈奎生.宫颈鳞癌组织中MTA1蛋白的表达及其意义[J].临床医学,2007, 27(9):77-78
5 Jang KS,Paik SS,Chung H,et al.MTA1 overexpression correlates significantly with tumor grade and angiogenesis in human breast cancer[J].Cancer Sci, 2006, 96(5):374-379
6阮洋,王景涛,高艳华,等. Livin、Caspase-3、MTA1在结肠癌组织中的表达及相关性的研究[J].中国老年学杂志,2007,27:1263-1265
7高冬玲,陈奎生,等.食管鳞状细胞癌组织中MTA1和E-cadher in蛋白的表达[J].郑州大学学报(医学版),2007,42(6):1028-1030
8 Matthias D,Rainer K,Sooryanarayana V,etal.The role of metastasis-associated protein 1 in prostate cancer progression[J].Cancer Research,2004,64:825-829
9周忠民,王禾,武国军,李海波.膀胱移行细胞癌组织中Mta-1与VEGF的表达及其相互关系[J].山西医科大学学报, 2008, 64(7):3
10汪盛,彭程,蒋先镇,等.肿瘤转移基因MTA1在膀胱癌中的表达及其临床意义[J].中国医学工程, 2007,55(5):3
11 Nicolson GL,Nawa A, Toh Y,et al.Tumor metastasis associated humanMTA1 gene and its MTA1 protein product: role in epithelial cancer cell invasion, proliferation and nuclear regulatione [J].Clin Exp Metastasis,2003,20(1):1924
12 Hofer MD, Menke A, Genze F, et al. Expression of MTA1 promotes motility and invasiveness of PANC1 pancreatic carcinoma cells [J]. Br J Cancer,2004, 90(2): 455-462
13 Jemal A,Siegel R,Ward E, et al.Cancer statistics, 2009. CA Cancer J Clin, 2009,59(4):225-249
14 Jemal A,Siegel R,Ward E,et al. Cancer statistics,2007 [J].CA Cancer J Clin, 2007,57(1):43-66
15 Guo JZH ,Shen JK,Zhou HY.The study of the function of elemene foranticancer [J]. Chinese Journal of Clinical Oncology,2003,30(10):752-754.
16陆羡,向金峰,等.β-榄香烯对K562细胞周期与细胞凋亡的影响极其机制探讨[J].中国小儿血液与肿瘤杂志,2008,13(4):149-152
17 Li X, Wang G,Zhao J,et al. Antiproliferative effect of beta-elemene in chemoresistant ovarian carcimoma cells is mediated through arrest of the cell cycle at the G2-M phase[J].Cell Mol Life,2005,62(7-8):894-904
18秦叔逵,钱军,杨爱珍,等.榄香烯乳抗肺癌细胞的实验研究[J].肿瘤防治研究,1996,23(4):251-255
19 Hacker G.The morphology of apoptosis[J].Cell Tissue Res,2000,301(1):5-17
20 Chipuk JE, Fisher JC, et al.Mechanism of apoptosis induction by inhibition of the anti-apoptotic BCL-2 proteins. Proc Natl Acad Sci J]. 2008,105(51):20327-20332
21 Jeen-Soo Bae,Jin Wook Park ,et al. Apoptotic cell death of human leukaemia U937 cells by ubiquinone-9 purified from Pleurotus eryngii[J].Natural Product Research, 2009,10(01):243-246
22 J.J. Pestka. Mechanisms of deoxynivalenol-induced gene expression and apoptosis[J].Food Additives & Contaminants A,2008,33(9):146-148
23 Kenji Sngamura, Masato Makino, et al, Pacliaxel-induced Aplptosis in Human Gastric Carcinoma Cell Lines[J]. Cancer,1996,77(1):14
24曹丽萍沈洪刘丽,等.黄芪甲苷、β-榄香烯对SGC7901胃癌细胞COX-及PGE-2表达的影响[J].现代中西医结合杂志,2010,19(7): 68-70
25陈春美,杨卫忠,王春华,等.榄香烯诱导人脑胶质瘤U251细胞凋亡及对端粒酶活性和hTERT表达影响的研究[J].中国肿瘤临床,2006,33(5):280-283
26陈光,丁晓飞,肖晋芳,等.榄香烯衍生物诱导Hela细胞凋亡的实验研究[J].中国药理学通报,2007,23(2):246-248
27吴大鹏,姜睿斌,赵德强,等.β-榄香烯对体外培养Tca-8113细胞放射增敏、细胞周期、凋亡及Bcl-2、Bax蛋白表达的影响[J].郑州大学学报(医学版),2009,44(3):414-417
28王茜莎,杨威,李明,等.β-榄香烯体内外抗肿瘤作用研究[J].中国药房,2009,20(9):650-653
29李传刚,李墨林,周琴,等.β-榄香烯对人膀胱癌BIU-87细胞磷脂膜功能及Bcl-2表达的影响[J].中草药,2007,38(6):886-889
30李传刚,李墨林,舒晓宏,等.β-榄香烯对人膀胱癌BIU-87细胞磷脂代谢转换率及细胞脂膜流动性的影响[J].中华医学杂志,2004,84(5):416-417
31 Toh Y, Pencil SD,Nicolson GL.A novel candidate metastasis-associated gene, mtal, differentially expressed in highly metastatic mammary adenocarcinoma cell lines.cDNA cloning, expression, and protein analyses,J Biol Chem, 1994, 269: 22958-22963
32 Cui Q, Takiguchi S, Matsusue K, et al. Assignment of the human metastasis-associated gene 1(MTA1) to human chromosome band 14q 32.3 by fluorescence in situ hybridization[J]. Cytogenet Cell Genet, 2001,93:139-140
33 Toh Y,Oki E, Oda S,et al. Analysis of the comp lete sequence of the novel metastasis associated candidate gene, mta1,differentially expressed in mammary adenocarcinoma and breast cancer cell lines[J].Gene,1995,159(1): 97-104
34 Zhang H, StephensLC, Kumar R. Metastasis tumor antigen family p roteins during breast cancer p rogression and metastasis in a reliable mouse model for human breast cancer [J].Clin Cancer Res, 2006,12(5):1479-1486
35 Nicolson GL,Nawa A,Toh Y,etal. Tumor metastasis-associated human MTA1 gene and its MTA1 product: role in epithelial cancer cell invasion, proliferation and nuclear regulation. Clin Exp Metastasis,2003,20(1):19-24
36 Sasaki H, Moriyama S, Nakashima Y, et al. Expression of the MTA1 mRNA in advanced lung cancer[J].Lung Cancer, 2002,35(2):149-154
38 Hofer MD, Kuefer R, Varambally S, et al. The role of metastasis associated protein 1 in prostate cancer progression[J].Cancer Res,2004,64(3): 825-829
39 Mahoney MG,et al.Metastasis-associated protein (MTA)1 enhances migration, invasion, and anchorage-independent survival of immortalized human keratinocytes [J]. Oncogene,2002,21(14): 2161-2170
40 Dales JP, Garcia S, Meunier-Carpentier S, et al. Overexp ression of hypoxia - inducible factor H IF - 1αpredicts early relapse in breast cancer: retrospective study in a series of 745 patients [J].Int JCancer,2005,116(5):734-739
41 De Craene B et al. The transcription factor snail induces tumor cell invasion through modulation of the epithelial cell differentiation program[J]. Cancer Res, 2005, 65(14):6237-6244
42唐正严,祖雄兵,齐琳,叶章群,周四维.膀胱移行细胞癌Mta-1基因与蛋白表达及其临床意义[J].临床泌尿外科杂志,2006,8(21):595-597
43周忠民,王禾,武国军,李海波.膀胱移行细胞癌组织中Mta-1与VEGF的表达及其相互关系[J].山西医科大学学报,2008,7(16):647-651
44汪盛,蒋先镇.转移相关基因1在膀胱癌中的表达及对膀胱癌细胞生物学行为的影响[J],2008,9(16):647-651
45徐培玉,等.榄香烯乳对浅表性膀胱癌治疗和预防术后复发的观察[J].中国肿瘤临床,2001,28(1):50
46常绍志,等.榄香烯乳膀胱内灌注治疗膀胱癌[J].中国肿瘤临床,1999, 26(1): 70
47谢锦来,蔡庆发,李励献,罗立君,谢建兵.Survivin在膀胱移行细胞组织癌中的表达及临床意义[J].海南医学院学报,2007,13(6):521-526
48 Tamm I, Wang Y, Sausville E, et al.IAP2family protein survivin inhibits caspase activity and apoptosis induced by Fas (CD95,Bax,caspases) and anticancer drugs[J].Cancer Res,1998,58(23): 5315-5320
49向彬,张福胤,吴佩春.β-榄香烯及顺铂对人涎腺腺样囊性癌SACC-83细胞毒作用的研究[J].现代口腔医学杂志,2005,19 (4):433-434
52朱秀美,刘屏.国内中药防治化疗药物毒副作用的研究概况.解放军药学学报,2001,17(6):321-323
1 Zheng S, Yang H, Zhang S, et al. Initial study on naturally occurring products from traditional Chinese herbs and vegetables for chemoprevention[J]. J Cell Biochem Suppl, 1997,27(5):106-112
2李广朝,等.β-榄香烯的基础研究与临床应用[J].长春中医药大学学报.2009,4(2):19-21
3杨骅,王仙平,郁琳琳,等.榄香烯抗癌作用与诱发肿瘤细胞凋亡[J].中华肿瘤杂志, 1996, 18(3):169-172
4范钰,林庚金,钱立平,等.榄香烯乳对胃癌SGC-7091细胞株端粒酶活性及细胞凋亡的影响[J].复旦学报(医学科学版), 2009, 28(1):5-8
5傅乃武,全兰萍,郭永,等.β-榄香烯的抗肿瘤作用和药理学研究[J].中药通报, 1984; 9(2):35-39
6秦叔逵,钱军,杨爱珍,等.榄香烯乳抗肺癌细胞的实验研究[J].肿瘤防治研究, 1996; 23(4):251-255
7郝立宏,卢步峰,于丽敏,等.β-榄香烯与阿霉素联合应用对CEM/APM细胞生长的影响[J].大连医科大学学报, 2009, 22(3):12
8鞠建峰,于维萍,等.β-榄香烯的现代研究及临床应用概况[J].齐鲁药事, 2008,38(4):34-37
9徐学军,周子成,罗元辉,等.β-榄香烯诱导人肝癌细胞株SMMC-7721凋亡的研究[J].第三军医大学学报, 1999, 21(4):268-271
10马东礼,童善庆.榄香烯对HeLa细胞端粒酶催化亚单位基因表达的作用[J].中国癌症杂志, 2009, 11(1):9-13
11 Hiyama K, Hiyama E, lshioka S, et al. Telomerase activity in small-cell and non-small-cell lung cancer[J] .J Natl Cancer Inst. 1995, 87(12):895-902.
12 Kerr J FR, Wyllie AH, Currie AR. Apoptosis: a basic biological phenomenon with wide ranging implication in tissue kinetics[J]. Br J Cancer, 2008, 26(3):239
13 Thompson CB. Apoptosis in the pathogenesis and treatment of disease[J]. Science, 2009, 267(52):1456-1462
14 Kasagi N, Somyo Y, Shirai H, et al. Al and cancer[J]. J cancer Res. 1994:85(9):939-945
15 Fulvio B, Lisa F, Alfredo F, et al. Potentiation of the malignant phenotype of the differentiated ARO thyroid cell line by insertion of the bcl-2 gene[J]. Int J cancer 1999, 81:956-962
16 Green DR. Apoptotic pathways:paper wraps stone blunts scissors[J].Cell, 2009, 102(31):112-115
17 Green DR, Reed Jc. Mitochondrion and apoptosls[J]. Science, 1998, 281(28):1309
18 Martinous Jc, Desagher S, Antonsson B,et al. Cytochrome C release from mitochondria: all or nothing[J]. Nature cell biology, 2000, 2:41-43
19 Li K, Li Y, Shelton JM, et al. Cytochrome C, deficiency causes embryonic lethality and attenuates stress-induced apoptosis[J]. Cell, 2008, 101(4):398-400
20 Ashkenazi A. Dixit VM. Death receptors:signaling and modulation[J]. Science, 1998, 281(28):1305
21袁静,顾振伦.榄香烯诱导K562细胞凋亡的流式细胞术分析[J].实用肿瘤杂志,2008,(6):343-344
22范钰,林庚金,钱立平,等.β-榄香烯对胃癌SGC7901细胞bcl-2基因表达及端粒酶活性的影响[J].上海医学,2001,24(8):490-492
23范钰,林庚金,钱立平,等.β-榄香烯对胃癌SGC-7901细胞C-myc基因表达及端粒酶活性的影响[J].中国肿瘤临床, 2001, 28(11): 848-851
24朱清静,熊振芳,等.β-榄香烯对肝星状细胞凋亡相关蛋白Bcl-2家族的影响[J].中国中西医消化杂志,2007,39(4):34-36
25刘宇男,王国良,贾永峰,等. MTT法在榄香烯乳对人膀胱癌T24细胞体外实验中的应用[J].上海医科大学学报,1997,24(2): 127-130
26李传刚,刘用楫,冯秉安,等.β-榄香烯对人BIU-87细胞诱导凋亡的实验研究[J].中国中西医结合外科杂志,2009,5(6):387-390
27李传刚,李墨林,周琴,等.β-榄香烯对人膀胱癌BIU-87细胞磷脂膜功能及Bcl-2表达的影响[J].中草药,2007,38(6):886-889
28钱军,秦叔逵,杨爱珍,等.榄香烯乳逆转人肺癌细胞的实验研究[J].肿瘤防治研究, 1996; 23(5): 299-301
29吴伟忠,刘康达,汤晓雷,等.β-榄香烯诱导的抗肿瘤免疫保护作用机理初探[J].中华肿瘤杂志, 1999, 21(6):405~408
30钱振超,刘金友,赵肃荣,等.莪术瘤苗的主动免疫保护效应-莪术抗癌作用原理的探讨[J].药学学报, 2008;16(12):892-896
31金梅,施广霞,朴花,等.榄香烯复合瘤苗主动免疫的抗肿瘤效应[J].中国肿瘤防治杂志, 1999, 6(1):50-51
32官成浓,何国章,等.榄香烯乳配合化疗对进展期胃癌患者免疫功能的影响[J].中国肿瘤临床, 2001, 28(2):123-124
33陈龙邦,藏静,王靖华,等.β-榄香烯对荷瘤小鼠免疫功能的影响[J].肿瘤防治研究, 1998, 25(1):54-56
34张学军,杜权,丁秀萍,等.榄香烯乳对r-DNA转录活性的影响及意义[J].中国肿瘤临床,1999, 26(6):443-444
35郦志军,周君富,陈周苗,等.榄香烯对肺癌患者血液SOD活性和LPO浓度的影响研究[J].中国肿瘤临床, 1998, 25(11): 825-853
36陈龙邦,藏静,王靖华,等.β-榄香烯对小鼠B16黑色素瘤细胞粘附,运动和间隙连接通讯功能的影响[J].肿瘤防治研究, 1999, 26(3):195-197
37李悦,杨向红,等.β-榄香烯对血管内皮细胞增殖及成血管能力的影响[J].山西医药杂志(下半月刊).2009,39(5):124-126
38郭建忠,张世渊,等.β-榄香烯诱导ECV-304细胞早期凋亡的实验研究[J].内蒙古名族大学学报.2009,46(12):117-119
39王宝成,郭军,狄剑时,等.榄香烯乳剂与肿瘤多药耐药的基础研究[J].中国肿瘤临床, 1996; 23(2):143-146
40 Gordon KJ,Blobe GC,et al.Role of transforming growth factor-beta superfamily signaling pathways in human disease[J].Biochim Biophys Acta 2008,1782(12):197-228
41 Zhao S, Venkatasubbarao K, Lazor JW,et al.Inhibition of STAT3 Tyr705 phosphorylation by Smad4 suppresses transforming growth factor beta-mediated invasion and metastasis in pancreatic cancer cells[J]. Cancer Res2008,68(35):4221-4228
42 Mahoney MG, Simpson A,Jost M, et al. Metastasis-associated protein (MTA)1 enhances migration, invasion, and anchorage-independent survival of immortalized human keratinocytes[J]. Oncogene, 2002, 21(14): 2161-70
43 Shiou SR, Singh AB, Moorthy K,et al. Smad4 regulates claudin-1 expression in a transforming growth factor-beta-independent manner in colon cancer cells[J]. Cancer Res 2007,67(5):1571-1579
44 Huang S, Zhang F, Miao L, et al. Lentiviral-mediated Smad4 RNAi induced antiproliferation by p16 up-regulation and apoptosis by caspase 3 down-regulation in hepatoma SMMC-7721 cells[J]. Oncol Rep 2008,20(2):1053-1059
45 Li X,Meng Y,Wu P,et al.Angiotensin II and Aldosterone stimulating NF-kappaB and AP-1 activation in hepatic fibrosis of rat[J]. Regul Pept.2007,138(1):15.
46 Liu J,Gong H,Zhang Z T,et al. Effect of angiotensin II and angiotensin II type 1 receptor antagonist on the proliferation,contraction and collagen synthesis in rat hepatic stellate cells[J]. ChinMed J(Engl),2008,121(2):161
47 MassaguéJ.TGFbeta in Cancer[J]. Cell.2008,134(12):215-230
48 Barros R, Pereira B, Duluc I,et al. Key elements of the BMP/ Smadpathway co-localize with CDX2 in intestinal metaplasia and regulate CDX2 expression in human gastric cell lines[J].J Pathol 2008,215(21):411-420
49 Zhao S, Venkatasubbarao K, Lazor JW,et al. Inhibition of STAT3 Tyr705 phosphorylation by Smad4 suppresses transforming growth factor beta-mediatedinvasion and metastasis in pancreatic cancer cells[J]. Cancer Res 2008,68(12):4221-4228
50刘妙玲,邹丽娟,崔桂敏,榄香烯乳增敏治疗骨转移癌60例临床研究[J]中国肿瘤临床学,2001,28(12):889-891
51佘军军,王子明,车向明,等.β-榄香烯对兔VX2肾癌放疗增敏作用中Caspase-3及Bcl-2的表达[J],第四军医大学学报(J FourthMilMed Univ) 2006; 27 (24)17-21
52魏新设,陈奎生.宫颈鳞癌组织中MTA1蛋白的表达及其意义[J].临床医学,2007, 27(9):77-78
53 Jang KS,Paik SS,Chung H,et al.MTA1 overexpression correlates significantly with tumor grade and angiogenesis in human breast cancer[J].Cancer Sci, 2006, 96(5):374-379
54阮洋,王景涛,高艳华,等. Livin、Caspase-3、MTA1在结肠癌组织中的表达及相关性的研究[J].中国老年学杂志,2007,27:1263-1265
55高冬玲,陈奎生,等.食管鳞状细胞癌组织中MTA1和E-cadher in蛋白的表达[J].郑州大学学报(医学版),2007,42(6):1028-1030
56 Matthias D,Rainer K,Sooryanarayana V,etal.The role of metastasis-associated protein 1 in prostate cancer progression[J].Cancer Research,2004,64:825-829
57 Toh Y, Pencil SD,Nicolson GL.A novel candidate metastasis-associated gene, mtal, differentially expressed in highly metastatic mammary adenocarcinoma cell lines.cDNA cloning, expression, and protein analyses,J Biol Chem, 1994, 269: 22958-22963
58 Cui Q, Takiguchi S, Matsusue K, et al. Assignment of the human metastasis-associated gene 1(MTA1) to human chromosome band 14q 32.3 by fluorescence in situ hybridization[J]. Cytogenet Cell Genet, 2001,93:139-140
59 Toh Y,Oki E, Oda S,et al. Analysis of the comp lete sequence of the novel metastasis associated candidate gene, mta1,differentially expressed in mammary adenocarcinoma and breast cancer cell lines[J].Gene,1995,159(1): 97-104
60 Zhang H, StephensLC, Kumar R. Metastasis tumor antigen family p roteins during breast cancer p rogression and metastasis in a reliable mouse model for humanbreast cancer [J].Clin Cancer Res, 2006,12(5):1479-1486
61 Nicolson GL,Nawa A,Toh Y,etal. Tumor metastasis-associated human MTA1 gene and its MTA1 product: role in epithelial cancer cell invasion, proliferation and nuclear regulation. Clin Exp Metastasis,2003,20(1):19-24
62 Sasaki H, Moriyama S, Nakashima Y, et al. Expression of the MTA1 mRNA in advanced lung cancer[J].Lung Cancer, 2002,35(2):149-154
2曹丽萍,沈洪,刘丽,等.黄芪甲苷、β-榄香烯对SGC7901胃癌细胞COX-及PGE-2表达的影响[J].现代中西医结合杂志,2010,19(7):68-70
3 Kumar R,Wang RA, Bagheri YR. Emerging roles of MTA1 family members in human cancers [J]. Semin Oncol,2003,30(5):3037
4魏新设,陈奎生.宫颈鳞癌组织中MTA1蛋白的表达及其意义[J].临床医学,2007, 27(9):77-78
5 Jang KS,Paik SS,Chung H,et al.MTA1 overexpression correlates significantly with tumor grade and angiogenesis in human breast cancer[J].Cancer Sci, 2006, 96(5):374-379
6阮洋,王景涛,高艳华,等. Livin、Caspase-3、MTA1在结肠癌组织中的表达及相关性的研究[J].中国老年学杂志,2007,27:1263-1265
7高冬玲,陈奎生,等.食管鳞状细胞癌组织中MTA1和E-cadher in蛋白的表达[J].郑州大学学报(医学版),2007,42(6):1028-1030
8 Matthias D,Rainer K,Sooryanarayana V,etal.The role of metastasis-associated protein 1 in prostate cancer progression[J].Cancer Research,2004,64:825-829
9周忠民,王禾,武国军,李海波.膀胱移行细胞癌组织中Mta-1与VEGF的表达及其相互关系[J].山西医科大学学报, 2008, 64(7):3
10汪盛,彭程,蒋先镇,等.肿瘤转移基因MTA1在膀胱癌中的表达及其临床意义[J].中国医学工程, 2007,55(5):3
11 Nicolson GL,Nawa A, Toh Y,et al.Tumor metastasis associated humanMTA1 gene and its MTA1 protein product: role in epithelial cancer cell invasion, proliferation and nuclear regulatione [J].Clin Exp Metastasis,2003,20(1):1924
12 Hofer MD, Menke A, Genze F, et al. Expression of MTA1 promotes motility and invasiveness of PANC1 pancreatic carcinoma cells [J]. Br J Cancer,2004, 90(2): 455-462
13 Jemal A,Siegel R,Ward E, et al.Cancer statistics, 2009. CA Cancer J Clin, 2009,59(4):225-249
14 Jemal A,Siegel R,Ward E,et al. Cancer statistics,2007 [J].CA Cancer J Clin, 2007,57(1):43-66
15 Guo JZH ,Shen JK,Zhou HY.The study of the function of elemene foranticancer [J]. Chinese Journal of Clinical Oncology,2003,30(10):752-754.
16陆羡,向金峰,等.β-榄香烯对K562细胞周期与细胞凋亡的影响极其机制探讨[J].中国小儿血液与肿瘤杂志,2008,13(4):149-152
17 Li X, Wang G,Zhao J,et al. Antiproliferative effect of beta-elemene in chemoresistant ovarian carcimoma cells is mediated through arrest of the cell cycle at the G2-M phase[J].Cell Mol Life,2005,62(7-8):894-904
18秦叔逵,钱军,杨爱珍,等.榄香烯乳抗肺癌细胞的实验研究[J].肿瘤防治研究,1996,23(4):251-255
19 Hacker G.The morphology of apoptosis[J].Cell Tissue Res,2000,301(1):5-17
20 Chipuk JE, Fisher JC, et al.Mechanism of apoptosis induction by inhibition of the anti-apoptotic BCL-2 proteins. Proc Natl Acad Sci J]. 2008,105(51):20327-20332
21 Jeen-Soo Bae,Jin Wook Park ,et al. Apoptotic cell death of human leukaemia U937 cells by ubiquinone-9 purified from Pleurotus eryngii[J].Natural Product Research, 2009,10(01):243-246
22 J.J. Pestka. Mechanisms of deoxynivalenol-induced gene expression and apoptosis[J].Food Additives & Contaminants A,2008,33(9):146-148
23 Kenji Sngamura, Masato Makino, et al, Pacliaxel-induced Aplptosis in Human Gastric Carcinoma Cell Lines[J]. Cancer,1996,77(1):14
24曹丽萍沈洪刘丽,等.黄芪甲苷、β-榄香烯对SGC7901胃癌细胞COX-及PGE-2表达的影响[J].现代中西医结合杂志,2010,19(7): 68-70
25陈春美,杨卫忠,王春华,等.榄香烯诱导人脑胶质瘤U251细胞凋亡及对端粒酶活性和hTERT表达影响的研究[J].中国肿瘤临床,2006,33(5):280-283
26陈光,丁晓飞,肖晋芳,等.榄香烯衍生物诱导Hela细胞凋亡的实验研究[J].中国药理学通报,2007,23(2):246-248
27吴大鹏,姜睿斌,赵德强,等.β-榄香烯对体外培养Tca-8113细胞放射增敏、细胞周期、凋亡及Bcl-2、Bax蛋白表达的影响[J].郑州大学学报(医学版),2009,44(3):414-417
28王茜莎,杨威,李明,等.β-榄香烯体内外抗肿瘤作用研究[J].中国药房,2009,20(9):650-653
29李传刚,李墨林,周琴,等.β-榄香烯对人膀胱癌BIU-87细胞磷脂膜功能及Bcl-2表达的影响[J].中草药,2007,38(6):886-889
30李传刚,李墨林,舒晓宏,等.β-榄香烯对人膀胱癌BIU-87细胞磷脂代谢转换率及细胞脂膜流动性的影响[J].中华医学杂志,2004,84(5):416-417
31 Toh Y, Pencil SD,Nicolson GL.A novel candidate metastasis-associated gene, mtal, differentially expressed in highly metastatic mammary adenocarcinoma cell lines.cDNA cloning, expression, and protein analyses,J Biol Chem, 1994, 269: 22958-22963
32 Cui Q, Takiguchi S, Matsusue K, et al. Assignment of the human metastasis-associated gene 1(MTA1) to human chromosome band 14q 32.3 by fluorescence in situ hybridization[J]. Cytogenet Cell Genet, 2001,93:139-140
33 Toh Y,Oki E, Oda S,et al. Analysis of the comp lete sequence of the novel metastasis associated candidate gene, mta1,differentially expressed in mammary adenocarcinoma and breast cancer cell lines[J].Gene,1995,159(1): 97-104
34 Zhang H, StephensLC, Kumar R. Metastasis tumor antigen family p roteins during breast cancer p rogression and metastasis in a reliable mouse model for human breast cancer [J].Clin Cancer Res, 2006,12(5):1479-1486
35 Nicolson GL,Nawa A,Toh Y,etal. Tumor metastasis-associated human MTA1 gene and its MTA1 product: role in epithelial cancer cell invasion, proliferation and nuclear regulation. Clin Exp Metastasis,2003,20(1):19-24
36 Sasaki H, Moriyama S, Nakashima Y, et al. Expression of the MTA1 mRNA in advanced lung cancer[J].Lung Cancer, 2002,35(2):149-154
38 Hofer MD, Kuefer R, Varambally S, et al. The role of metastasis associated protein 1 in prostate cancer progression[J].Cancer Res,2004,64(3): 825-829
39 Mahoney MG,et al.Metastasis-associated protein (MTA)1 enhances migration, invasion, and anchorage-independent survival of immortalized human keratinocytes [J]. Oncogene,2002,21(14): 2161-2170
40 Dales JP, Garcia S, Meunier-Carpentier S, et al. Overexp ression of hypoxia - inducible factor H IF - 1αpredicts early relapse in breast cancer: retrospective study in a series of 745 patients [J].Int JCancer,2005,116(5):734-739
41 De Craene B et al. The transcription factor snail induces tumor cell invasion through modulation of the epithelial cell differentiation program[J]. Cancer Res, 2005, 65(14):6237-6244
42唐正严,祖雄兵,齐琳,叶章群,周四维.膀胱移行细胞癌Mta-1基因与蛋白表达及其临床意义[J].临床泌尿外科杂志,2006,8(21):595-597
43周忠民,王禾,武国军,李海波.膀胱移行细胞癌组织中Mta-1与VEGF的表达及其相互关系[J].山西医科大学学报,2008,7(16):647-651
44汪盛,蒋先镇.转移相关基因1在膀胱癌中的表达及对膀胱癌细胞生物学行为的影响[J],2008,9(16):647-651
45徐培玉,等.榄香烯乳对浅表性膀胱癌治疗和预防术后复发的观察[J].中国肿瘤临床,2001,28(1):50
46常绍志,等.榄香烯乳膀胱内灌注治疗膀胱癌[J].中国肿瘤临床,1999, 26(1): 70
47谢锦来,蔡庆发,李励献,罗立君,谢建兵.Survivin在膀胱移行细胞组织癌中的表达及临床意义[J].海南医学院学报,2007,13(6):521-526
48 Tamm I, Wang Y, Sausville E, et al.IAP2family protein survivin inhibits caspase activity and apoptosis induced by Fas (CD95,Bax,caspases) and anticancer drugs[J].Cancer Res,1998,58(23): 5315-5320
49向彬,张福胤,吴佩春.β-榄香烯及顺铂对人涎腺腺样囊性癌SACC-83细胞毒作用的研究[J].现代口腔医学杂志,2005,19 (4):433-434
52朱秀美,刘屏.国内中药防治化疗药物毒副作用的研究概况.解放军药学学报,2001,17(6):321-323
1 Zheng S, Yang H, Zhang S, et al. Initial study on naturally occurring products from traditional Chinese herbs and vegetables for chemoprevention[J]. J Cell Biochem Suppl, 1997,27(5):106-112
2李广朝,等.β-榄香烯的基础研究与临床应用[J].长春中医药大学学报.2009,4(2):19-21
3杨骅,王仙平,郁琳琳,等.榄香烯抗癌作用与诱发肿瘤细胞凋亡[J].中华肿瘤杂志, 1996, 18(3):169-172
4范钰,林庚金,钱立平,等.榄香烯乳对胃癌SGC-7091细胞株端粒酶活性及细胞凋亡的影响[J].复旦学报(医学科学版), 2009, 28(1):5-8
5傅乃武,全兰萍,郭永,等.β-榄香烯的抗肿瘤作用和药理学研究[J].中药通报, 1984; 9(2):35-39
6秦叔逵,钱军,杨爱珍,等.榄香烯乳抗肺癌细胞的实验研究[J].肿瘤防治研究, 1996; 23(4):251-255
7郝立宏,卢步峰,于丽敏,等.β-榄香烯与阿霉素联合应用对CEM/APM细胞生长的影响[J].大连医科大学学报, 2009, 22(3):12
8鞠建峰,于维萍,等.β-榄香烯的现代研究及临床应用概况[J].齐鲁药事, 2008,38(4):34-37
9徐学军,周子成,罗元辉,等.β-榄香烯诱导人肝癌细胞株SMMC-7721凋亡的研究[J].第三军医大学学报, 1999, 21(4):268-271
10马东礼,童善庆.榄香烯对HeLa细胞端粒酶催化亚单位基因表达的作用[J].中国癌症杂志, 2009, 11(1):9-13
11 Hiyama K, Hiyama E, lshioka S, et al. Telomerase activity in small-cell and non-small-cell lung cancer[J] .J Natl Cancer Inst. 1995, 87(12):895-902.
12 Kerr J FR, Wyllie AH, Currie AR. Apoptosis: a basic biological phenomenon with wide ranging implication in tissue kinetics[J]. Br J Cancer, 2008, 26(3):239
13 Thompson CB. Apoptosis in the pathogenesis and treatment of disease[J]. Science, 2009, 267(52):1456-1462
14 Kasagi N, Somyo Y, Shirai H, et al. Al and cancer[J]. J cancer Res. 1994:85(9):939-945
15 Fulvio B, Lisa F, Alfredo F, et al. Potentiation of the malignant phenotype of the differentiated ARO thyroid cell line by insertion of the bcl-2 gene[J]. Int J cancer 1999, 81:956-962
16 Green DR. Apoptotic pathways:paper wraps stone blunts scissors[J].Cell, 2009, 102(31):112-115
17 Green DR, Reed Jc. Mitochondrion and apoptosls[J]. Science, 1998, 281(28):1309
18 Martinous Jc, Desagher S, Antonsson B,et al. Cytochrome C release from mitochondria: all or nothing[J]. Nature cell biology, 2000, 2:41-43
19 Li K, Li Y, Shelton JM, et al. Cytochrome C, deficiency causes embryonic lethality and attenuates stress-induced apoptosis[J]. Cell, 2008, 101(4):398-400
20 Ashkenazi A. Dixit VM. Death receptors:signaling and modulation[J]. Science, 1998, 281(28):1305
21袁静,顾振伦.榄香烯诱导K562细胞凋亡的流式细胞术分析[J].实用肿瘤杂志,2008,(6):343-344
22范钰,林庚金,钱立平,等.β-榄香烯对胃癌SGC7901细胞bcl-2基因表达及端粒酶活性的影响[J].上海医学,2001,24(8):490-492
23范钰,林庚金,钱立平,等.β-榄香烯对胃癌SGC-7901细胞C-myc基因表达及端粒酶活性的影响[J].中国肿瘤临床, 2001, 28(11): 848-851
24朱清静,熊振芳,等.β-榄香烯对肝星状细胞凋亡相关蛋白Bcl-2家族的影响[J].中国中西医消化杂志,2007,39(4):34-36
25刘宇男,王国良,贾永峰,等. MTT法在榄香烯乳对人膀胱癌T24细胞体外实验中的应用[J].上海医科大学学报,1997,24(2): 127-130
26李传刚,刘用楫,冯秉安,等.β-榄香烯对人BIU-87细胞诱导凋亡的实验研究[J].中国中西医结合外科杂志,2009,5(6):387-390
27李传刚,李墨林,周琴,等.β-榄香烯对人膀胱癌BIU-87细胞磷脂膜功能及Bcl-2表达的影响[J].中草药,2007,38(6):886-889
28钱军,秦叔逵,杨爱珍,等.榄香烯乳逆转人肺癌细胞的实验研究[J].肿瘤防治研究, 1996; 23(5): 299-301
29吴伟忠,刘康达,汤晓雷,等.β-榄香烯诱导的抗肿瘤免疫保护作用机理初探[J].中华肿瘤杂志, 1999, 21(6):405~408
30钱振超,刘金友,赵肃荣,等.莪术瘤苗的主动免疫保护效应-莪术抗癌作用原理的探讨[J].药学学报, 2008;16(12):892-896
31金梅,施广霞,朴花,等.榄香烯复合瘤苗主动免疫的抗肿瘤效应[J].中国肿瘤防治杂志, 1999, 6(1):50-51
32官成浓,何国章,等.榄香烯乳配合化疗对进展期胃癌患者免疫功能的影响[J].中国肿瘤临床, 2001, 28(2):123-124
33陈龙邦,藏静,王靖华,等.β-榄香烯对荷瘤小鼠免疫功能的影响[J].肿瘤防治研究, 1998, 25(1):54-56
34张学军,杜权,丁秀萍,等.榄香烯乳对r-DNA转录活性的影响及意义[J].中国肿瘤临床,1999, 26(6):443-444
35郦志军,周君富,陈周苗,等.榄香烯对肺癌患者血液SOD活性和LPO浓度的影响研究[J].中国肿瘤临床, 1998, 25(11): 825-853
36陈龙邦,藏静,王靖华,等.β-榄香烯对小鼠B16黑色素瘤细胞粘附,运动和间隙连接通讯功能的影响[J].肿瘤防治研究, 1999, 26(3):195-197
37李悦,杨向红,等.β-榄香烯对血管内皮细胞增殖及成血管能力的影响[J].山西医药杂志(下半月刊).2009,39(5):124-126
38郭建忠,张世渊,等.β-榄香烯诱导ECV-304细胞早期凋亡的实验研究[J].内蒙古名族大学学报.2009,46(12):117-119
39王宝成,郭军,狄剑时,等.榄香烯乳剂与肿瘤多药耐药的基础研究[J].中国肿瘤临床, 1996; 23(2):143-146
40 Gordon KJ,Blobe GC,et al.Role of transforming growth factor-beta superfamily signaling pathways in human disease[J].Biochim Biophys Acta 2008,1782(12):197-228
41 Zhao S, Venkatasubbarao K, Lazor JW,et al.Inhibition of STAT3 Tyr705 phosphorylation by Smad4 suppresses transforming growth factor beta-mediated invasion and metastasis in pancreatic cancer cells[J]. Cancer Res2008,68(35):4221-4228
42 Mahoney MG, Simpson A,Jost M, et al. Metastasis-associated protein (MTA)1 enhances migration, invasion, and anchorage-independent survival of immortalized human keratinocytes[J]. Oncogene, 2002, 21(14): 2161-70
43 Shiou SR, Singh AB, Moorthy K,et al. Smad4 regulates claudin-1 expression in a transforming growth factor-beta-independent manner in colon cancer cells[J]. Cancer Res 2007,67(5):1571-1579
44 Huang S, Zhang F, Miao L, et al. Lentiviral-mediated Smad4 RNAi induced antiproliferation by p16 up-regulation and apoptosis by caspase 3 down-regulation in hepatoma SMMC-7721 cells[J]. Oncol Rep 2008,20(2):1053-1059
45 Li X,Meng Y,Wu P,et al.Angiotensin II and Aldosterone stimulating NF-kappaB and AP-1 activation in hepatic fibrosis of rat[J]. Regul Pept.2007,138(1):15.
46 Liu J,Gong H,Zhang Z T,et al. Effect of angiotensin II and angiotensin II type 1 receptor antagonist on the proliferation,contraction and collagen synthesis in rat hepatic stellate cells[J]. ChinMed J(Engl),2008,121(2):161
47 MassaguéJ.TGFbeta in Cancer[J]. Cell.2008,134(12):215-230
48 Barros R, Pereira B, Duluc I,et al. Key elements of the BMP/ Smadpathway co-localize with CDX2 in intestinal metaplasia and regulate CDX2 expression in human gastric cell lines[J].J Pathol 2008,215(21):411-420
49 Zhao S, Venkatasubbarao K, Lazor JW,et al. Inhibition of STAT3 Tyr705 phosphorylation by Smad4 suppresses transforming growth factor beta-mediatedinvasion and metastasis in pancreatic cancer cells[J]. Cancer Res 2008,68(12):4221-4228
50刘妙玲,邹丽娟,崔桂敏,榄香烯乳增敏治疗骨转移癌60例临床研究[J]中国肿瘤临床学,2001,28(12):889-891
51佘军军,王子明,车向明,等.β-榄香烯对兔VX2肾癌放疗增敏作用中Caspase-3及Bcl-2的表达[J],第四军医大学学报(J FourthMilMed Univ) 2006; 27 (24)17-21
52魏新设,陈奎生.宫颈鳞癌组织中MTA1蛋白的表达及其意义[J].临床医学,2007, 27(9):77-78
53 Jang KS,Paik SS,Chung H,et al.MTA1 overexpression correlates significantly with tumor grade and angiogenesis in human breast cancer[J].Cancer Sci, 2006, 96(5):374-379
54阮洋,王景涛,高艳华,等. Livin、Caspase-3、MTA1在结肠癌组织中的表达及相关性的研究[J].中国老年学杂志,2007,27:1263-1265
55高冬玲,陈奎生,等.食管鳞状细胞癌组织中MTA1和E-cadher in蛋白的表达[J].郑州大学学报(医学版),2007,42(6):1028-1030
56 Matthias D,Rainer K,Sooryanarayana V,etal.The role of metastasis-associated protein 1 in prostate cancer progression[J].Cancer Research,2004,64:825-829
57 Toh Y, Pencil SD,Nicolson GL.A novel candidate metastasis-associated gene, mtal, differentially expressed in highly metastatic mammary adenocarcinoma cell lines.cDNA cloning, expression, and protein analyses,J Biol Chem, 1994, 269: 22958-22963
58 Cui Q, Takiguchi S, Matsusue K, et al. Assignment of the human metastasis-associated gene 1(MTA1) to human chromosome band 14q 32.3 by fluorescence in situ hybridization[J]. Cytogenet Cell Genet, 2001,93:139-140
59 Toh Y,Oki E, Oda S,et al. Analysis of the comp lete sequence of the novel metastasis associated candidate gene, mta1,differentially expressed in mammary adenocarcinoma and breast cancer cell lines[J].Gene,1995,159(1): 97-104
60 Zhang H, StephensLC, Kumar R. Metastasis tumor antigen family p roteins during breast cancer p rogression and metastasis in a reliable mouse model for humanbreast cancer [J].Clin Cancer Res, 2006,12(5):1479-1486
61 Nicolson GL,Nawa A,Toh Y,etal. Tumor metastasis-associated human MTA1 gene and its MTA1 product: role in epithelial cancer cell invasion, proliferation and nuclear regulation. Clin Exp Metastasis,2003,20(1):19-24
62 Sasaki H, Moriyama S, Nakashima Y, et al. Expression of the MTA1 mRNA in advanced lung cancer[J].Lung Cancer, 2002,35(2):149-154