中药贝母多样性研究及其质量考察
|
摘要
本文参考了有关中药贝母分布、分类及药用资源的研究资料,运用可见-紫外分光光度法及HPLC法进行质量分析、评价及化学成分多样性比较,对贝母种的确定和种的多样性进行探索研究。
1.从品种考证、活性成分含量测定、功效及药理作用等方面对贝母进行综述,为进一步开发贝母药用资源提供了基础研究资料。
2.通过查阅大量文献资料,从基源和地理分布等方面对中药贝母多样性资料进行研究。国内中药贝母的植物来源比较复杂。除药典收载的贝母植物来源外,各地习用品的植物来源也多种多样。甘肃省作为贝母的一个产区,广泛分布着具有利用价值的种类。经过文献研究,确定甘肃贝母属药用植物有7种。
3.采用高效液相色谱法对平贝母、浙贝母中水溶性成分的指纹图谱进行了初步研究。结果表明,平贝母与浙贝母的共有模式具有明显的相似性。平贝母、浙贝母中所含的非共有峰,表明平贝母、浙贝母样品在化学成分组成上是存在多样性,这对于中药贝母鉴别、分类、质量评价有重要的意义。
4.采用HPLC-ELSD法同时测定市售平贝母、浙贝母中贝母素甲、贝母素乙的含量,结果表明,贝母素甲、贝母素乙在0.058～0.348μg和0.084～0.504μg范围内线性关系良好。平均回收率(n=6)分别为98.43%,98.87%,RSD分别为1.79%,1.94%。根据测定结果建议平贝母中贝母素甲和贝母素乙的含量总和应不少于0.007%。此法简便,快捷,结果可靠。
5.采用酸性染料比色法测定市售平贝母、浙贝母中总生物碱的含量。测定结果表明,浙贝母中总生物碱的含量平均值明显高于平贝母中总生物碱的含量。为进一步全面评价贝母的质量、品种鉴别提供依据。可作为贝母中总生物碱含量测定的实用技术。
6.采用高效液相色谱法测定平贝母、浙贝母中腺苷的含量,结果表明,腺苷在0.040～0.240μg与峰面积线性关系良好(r=0.9999),平均加样回收率为96.32%,RSD=1.15%(n=5)。贝母中腺苷的含量比较丰富,平贝母、浙贝母中腺苷的平均含量没有明显的差异。方法简便、准确、重复性好。
本论文主要结论为:中药贝母有多方面的多样性,存在较大的潜在价值,应加大对中药贝母资源的研究。
1.通过高效液相色谱法进行指纹图谱研究得到平贝母与浙贝母的水溶性成分共有模式具有明显的相似性,在此研究基础上,认为平贝母可以当浙贝母使用。
2.中药贝母有两大类:一是川贝母,主要功效是清热润肺、化痰止咳;二是浙贝母,主要功效是清热散结、化痰止咳。其它各类贝母,均分别作为这两种贝母的代用品。平贝母则通常作为川贝母使用。本实验结果则显示,平贝母与浙贝母相似性很好。
This article made reference to the research materials of Fritillaria,studied the distribution,classification and medicinal resources of Fritillaria,by using UV spectrophotometry and HPLC method to analyse and evaluate the quality and study of diversity of Bulbus Fritillaria.
1.In this paper,we summaried Fritillaria from their varieties of research,the active ingredients,determination,efficacy,Pharmacological effects and other aspects, and supplied a basis research data for further developing of medicinal plants of Fritillaria.
2.Through accessing to a large number of documents,we studied biodiversity information of Fritillaria from the original base and geographical distribution.It showed that Plant sources of Fritillaria were complex.As a region,Gansu had certain useful species,after studying the documents,we believed Gansu had 7 kinds of medicinal plants of Fritillaria.
3.With HPLC method,did some works on building up fingerprint of water-solubility components in Bulbus Fritillariae.The results showed we could evaluate other sample's quality with similarity on the basis of standard fingerprint established.According to the non-total peaks in Bulbus Fritillaria,we can see Bulbus Fritillaria exist diversity in the chemical composition,this has a great significance for identification,classification and quality evaluation of traditional Chinese medicine Bulbus Fritillaria.
4.Determination of verticine and verticinone in Bulbus Fritillaria by HPLC-ELSD,The calibration curves for verticine and verticinone were linear within the range of 0.058～0.348μg and 0.084～0.504μg respectively.The recoveries were 98.43%and 98.87%with RSD 1.79%and 1.94%(n=6) respectively.Based on the above results,we suggested the sum contents of verticine and verticinone in Bulbus Fritillaria ussuriensis were not less than0.007%,The developed method was simple, rapid and reliable.
5.Determination of total alkaloids in Bulbus Fritillaria by Acid dye colorimetry. The results showed that the total alkaloids in Bulbus Fritillaria thunbergii were significantly higher than that in Bulbus Fritillaria ussuriensis.This provided a basis for comprehensively evaluating the quality of Bulbus Fritillaria and for the species identification.This method can be used as a practical technology for the determination of total alkaloids in Bulbus Fritillaria.
6.Determination of adenosine in Bulbus Fritillaria by HPLC,The method was simple,accurate and had a good reproducibility.The linear was good in the range of 0.040～0.240μg(r=0.9999)with average recovery of 96.32%and RSD of 1.15%(n= 5).The content of adenosine in Bulbus Fritillaria was relatively rich.The average content of adenosine had not significant difference in the two Chinese herbal medicine.
Chief conclusion:Fritillaria is a traditional Chinese medicine,it has a wide range of diversity,the resources of it must be increasely studied.
1.With HPLC method,we found the fingerprints of water-solubility components of Fritillariae ussuriensis and Fritillaria thunbergii had obvious similarities.In this research,we think Fritillaria ussuriensis can be used as Fritillaria thunbergii.
2.Fritillaria has two categories:the first is Bulbus Fritillaria cirrhosa,the second is Bulbus Fritillaria thunbergii.The other Fritillarias,both as a substitute for the two Fritillarias.Fritillaria ussuriensis is often used as Fritillaria cirrhosa.The results showed Fritillaria ussuriensis and Fritillaria thunbergii had a good similarity.
1.从品种考证、活性成分含量测定、功效及药理作用等方面对贝母进行综述,为进一步开发贝母药用资源提供了基础研究资料。
2.通过查阅大量文献资料,从基源和地理分布等方面对中药贝母多样性资料进行研究。国内中药贝母的植物来源比较复杂。除药典收载的贝母植物来源外,各地习用品的植物来源也多种多样。甘肃省作为贝母的一个产区,广泛分布着具有利用价值的种类。经过文献研究,确定甘肃贝母属药用植物有7种。
3.采用高效液相色谱法对平贝母、浙贝母中水溶性成分的指纹图谱进行了初步研究。结果表明,平贝母与浙贝母的共有模式具有明显的相似性。平贝母、浙贝母中所含的非共有峰,表明平贝母、浙贝母样品在化学成分组成上是存在多样性,这对于中药贝母鉴别、分类、质量评价有重要的意义。
4.采用HPLC-ELSD法同时测定市售平贝母、浙贝母中贝母素甲、贝母素乙的含量,结果表明,贝母素甲、贝母素乙在0.058～0.348μg和0.084～0.504μg范围内线性关系良好。平均回收率(n=6)分别为98.43%,98.87%,RSD分别为1.79%,1.94%。根据测定结果建议平贝母中贝母素甲和贝母素乙的含量总和应不少于0.007%。此法简便,快捷,结果可靠。
5.采用酸性染料比色法测定市售平贝母、浙贝母中总生物碱的含量。测定结果表明,浙贝母中总生物碱的含量平均值明显高于平贝母中总生物碱的含量。为进一步全面评价贝母的质量、品种鉴别提供依据。可作为贝母中总生物碱含量测定的实用技术。
6.采用高效液相色谱法测定平贝母、浙贝母中腺苷的含量,结果表明,腺苷在0.040～0.240μg与峰面积线性关系良好(r=0.9999),平均加样回收率为96.32%,RSD=1.15%(n=5)。贝母中腺苷的含量比较丰富,平贝母、浙贝母中腺苷的平均含量没有明显的差异。方法简便、准确、重复性好。
本论文主要结论为:中药贝母有多方面的多样性,存在较大的潜在价值,应加大对中药贝母资源的研究。
1.通过高效液相色谱法进行指纹图谱研究得到平贝母与浙贝母的水溶性成分共有模式具有明显的相似性,在此研究基础上,认为平贝母可以当浙贝母使用。
2.中药贝母有两大类:一是川贝母,主要功效是清热润肺、化痰止咳;二是浙贝母,主要功效是清热散结、化痰止咳。其它各类贝母,均分别作为这两种贝母的代用品。平贝母则通常作为川贝母使用。本实验结果则显示,平贝母与浙贝母相似性很好。
This article made reference to the research materials of Fritillaria,studied the distribution,classification and medicinal resources of Fritillaria,by using UV spectrophotometry and HPLC method to analyse and evaluate the quality and study of diversity of Bulbus Fritillaria.
1.In this paper,we summaried Fritillaria from their varieties of research,the active ingredients,determination,efficacy,Pharmacological effects and other aspects, and supplied a basis research data for further developing of medicinal plants of Fritillaria.
2.Through accessing to a large number of documents,we studied biodiversity information of Fritillaria from the original base and geographical distribution.It showed that Plant sources of Fritillaria were complex.As a region,Gansu had certain useful species,after studying the documents,we believed Gansu had 7 kinds of medicinal plants of Fritillaria.
3.With HPLC method,did some works on building up fingerprint of water-solubility components in Bulbus Fritillariae.The results showed we could evaluate other sample's quality with similarity on the basis of standard fingerprint established.According to the non-total peaks in Bulbus Fritillaria,we can see Bulbus Fritillaria exist diversity in the chemical composition,this has a great significance for identification,classification and quality evaluation of traditional Chinese medicine Bulbus Fritillaria.
4.Determination of verticine and verticinone in Bulbus Fritillaria by HPLC-ELSD,The calibration curves for verticine and verticinone were linear within the range of 0.058～0.348μg and 0.084～0.504μg respectively.The recoveries were 98.43%and 98.87%with RSD 1.79%and 1.94%(n=6) respectively.Based on the above results,we suggested the sum contents of verticine and verticinone in Bulbus Fritillaria ussuriensis were not less than0.007%,The developed method was simple, rapid and reliable.
5.Determination of total alkaloids in Bulbus Fritillaria by Acid dye colorimetry. The results showed that the total alkaloids in Bulbus Fritillaria thunbergii were significantly higher than that in Bulbus Fritillaria ussuriensis.This provided a basis for comprehensively evaluating the quality of Bulbus Fritillaria and for the species identification.This method can be used as a practical technology for the determination of total alkaloids in Bulbus Fritillaria.
6.Determination of adenosine in Bulbus Fritillaria by HPLC,The method was simple,accurate and had a good reproducibility.The linear was good in the range of 0.040～0.240μg(r=0.9999)with average recovery of 96.32%and RSD of 1.15%(n= 5).The content of adenosine in Bulbus Fritillaria was relatively rich.The average content of adenosine had not significant difference in the two Chinese herbal medicine.
Chief conclusion:Fritillaria is a traditional Chinese medicine,it has a wide range of diversity,the resources of it must be increasely studied.
1.With HPLC method,we found the fingerprints of water-solubility components of Fritillariae ussuriensis and Fritillaria thunbergii had obvious similarities.In this research,we think Fritillaria ussuriensis can be used as Fritillaria thunbergii.
2.Fritillaria has two categories:the first is Bulbus Fritillaria cirrhosa,the second is Bulbus Fritillaria thunbergii.The other Fritillarias,both as a substitute for the two Fritillarias.Fritillaria ussuriensis is often used as Fritillaria cirrhosa.The results showed Fritillaria ussuriensis and Fritillaria thunbergii had a good similarity.
引文
[1]文化部古文献研究室等,阜阳汉简·万物,文物.1988,(4):36
[2]朱喜,注.诗经.上海:上海古籍出版社,1988.23
[3]郝懿行.尔雅义疏(下一).北京市中国书店铺:释草24
[4]赵学敏.本草纲目拾遗[M].第2版.人民卫生出版社,1983.123
[5]谢志民,王敏春,吕润霞.贝母类中药品种的本草考证[J].中药材,2000,23(7).
[6]谢宗万.中药材品种论述[M].第2版(上册).上海科学技术出版社,1990.388
[7]张建兴.浙贝母新鲜鳞茎化学成分的研究[J].中国中药杂志,1993,18(6):354-355
[8]马鸿年.川贝母商品松贝和青贝的生物碱含量[J].中国中药杂志,1995,20(3)
[9]刘庆华.新疆贝母化学成分研究[J].药学学报,1984,19(12):894
[10]徐东铭.伊贝母生物碱的研究[J].植物学报,1990,32
[11]徐东铭,张本,祁彦.平贝母生物碱研究Ⅱ[J].中草药,1983,14(2):7-8
[12]李兴斌,高燕飞,李吉良.平贝母化学成分及药理活性研究进展[J].中医药信息,2004.21(4)
[13]Kitamura:Y,et,al.Tetraberdron Lett.1988,45(18):5755-5758
[14]Kitamura:Y,et,al.Tennen Yukikagobustsu Toronkaikoen Yashishu.1989,31(52):6829-6832
[15]Kitamura:Y,et,al.Tetraberdron Lett.1989,30(3):4981-4984
[16]Kitamura:Y,et,al.Tetraberdron Lett.1989,45(23):7281-7283
[17]CA1990,112:14555LW
[18]肖培根.湖北贝母的研究进展[J].中国中药杂志,2002,27(10):726
[19]Kitajima J,Komori T,Kawasaki T,et al.Studies on the Constituents of Crude Drug "Fritillariae Bulbus"[J].Chem Pharm Bull,1982,30:3912-3921
[20]Kitajima J,Noda T N,Ida Y,et al.Studies on the Constituents of Crude Drug "Fritillariae Bulbus"Ⅳ[J].Chem Pharm Bull,1982,30:3922-3931
[21]北岛润一,古森微哉,川珐敏男.Studies on the Constituents of Crude Drug "Fritillariae
[22]北岛润一,伊田喜光,野田直希,等.Studies on the constituents of Crude Drug "Fritillariae Bulbus"Ⅵ[J].药学杂志,1982,102:1016-1022
[23]陈泽乃,陆阳,徐佩娟等.中药贝母中水溶性成分的研究[J].中国中药杂志,1996,21 (7):420-424
[24]沙世炎.中草药有效成分分析法[M](上册).北京:人民卫生出版社,1982.59
[25]李萍,曾令杰,李松林.无紫外吸收的贝母总生物碱定量分析方法研究[J].中国药学杂志,2002,37(8)
[26]王冲之,孙健,李萍.贝母类药材生物碱及生物碱苷含量测定方法学研究[J].中国药学杂志,2003,38(6)
[27]徐汝明,刘海卫,陆阳等.双波长紫外分光光度法测定贝母中腺苷和胸苷的含量[J].药学学报,1997,32(8):617-619
[28]薛燕,王峰.不同产地浙贝母药材中3种活性成分的分析研究[J].中国中药杂志,2007,32(16):1628-1630
[29]闫明,仲婕,刘庆华等.十种贝母裂解气相色谱分析鉴定[J].中国现代应用药学,1996,13(5):19-21
[30]张立群,熊俭,付秉昌..双波长薄层扫描法测定湖北贝母中湖贝甲素的含量[J].中国药师,1999,2(3):143-144
[31]张鹏,徐仿周.贝母及其复方制剂中生物碱含量测定方法[J].医药导报.2004,23(4):262
[32]姜艳,李萍.HPLC-ELSD测定浙贝母中贝母素甲、贝母素乙的含量[J].中国药学杂志,2005,40(16):1259
[33]谢志民,吕润霞.贝母类中药功效订正[J].中药材,2001,24(8):600-602
[34]于晓琳,季晖,王长礼等.贝母的药理作用研究概况[J].中草药,2000,31(4):313-314
[35]姚丽娜,孙汉清,江湛等.湖北贝母、鄂北贝母、紫花鄂北贝母总碱对呼吸系统的药理作用[J].同济医科大学学报,1993,22(1):47-49
[36]Chen K K,et al1.Chinese J Physiology.1935,9(1):21
[37]Siitsul K,et al1.JPn1 Kokai Tokkvo Koho JP 62,164,622[87,164,622]
[38]silsu K,et al1.生药学杂志(日).1987,41(3):174
[39]张勇慧,阮汉利,吴继洲.贝母的药理作用研究概况[J].医药导报,2003,22(11)
[40]张鹏,皮慧芳,张久亮等.湖北贝母茎叶中的生物碱类成分研究[J].中草药,2008,39(9):1294-1296
[41]张久亮,皮慧芳,阮汉利等.湖北贝母茎叶非生物碱成分的研究[J].医药导报,2007,26(1):23-25
[42]汪殿蓓,暨淑仪,陈飞鹏.植物群落物种多样性研究综述[J].生态学杂志,2001,20(4):55
[43]贺金生,马克平.物种多样性[A].见:蒋志刚.保护生物学[C].杭州:杭州科学技术出版社,1997.20-33
[44]林长松,左经会,廖雯.稀有植物十齿花群落物种多样性研究[J].植物研究,2008,28(3)
[45]余世春,肖培根.贝母属植物异甾体生物碱的存在及其分类学意义[J].植物分类学报,1992,30(5):450
[46]中国科学院中国植物志编辑委员会.《中国植物志》.北京:科学出版社,1988
[47]中国药典[S].2005年版.一部
[48]王天志,杜蕾蕾.川贝母的研究进展[J].华西药学杂志,2001,16(3):200
[49]李萍,徐国钧.中药贝母类的资源利用研究[J].植物资源与环境,1993,2(3)12-17
[50]何斜.中药贝母的品种与性状区别[J].海峡药学,2002,14(6):55
[51]唐进,汪发缵.中国植物志,十四卷.北京:科学出版社,1980.97-116
[52]陈心放,余国奠,周印锁.甘肃贝母属新植物[J].云南植物研究,1985,7(2):146-150
[53]杨永康,吴家坤.国产贝母属的新分类群[J].西北植物学报,1985,5(1):26-40
[54]张耀甲,程林.甘肃贝母属植物分类学研究[J].兰州大学学报(自然科学版),1998,34(2):84-85
[55]张恩和,黄高宝,景锐.西北药用植物资源多样性保护与持续利用[J].甘肃科学学报,1997,9(1):43-44
[56]庄起明,马骥,李俊祯.甘肃药用植物资源多样性及其保护利用[J].中国野生植物资源,2003,22(1):11-13
[57]刘丽莎.药用植物遗传多样性的保护和利用[J].甘肃中医学院学报,1999,(3):60-62
[58]马克平,钱迎倩.生物多样性保护及其研究进展[J].应用与环境生物学报,1998,4(1):95-99
[59]王永刚,吴忠,魏凤环等.中药指纹图谱研究的现状与未来[J].中药材,2003,26(11):823-825
[60]孙庆雷,林云良,祝贺等.HPLC指纹图谱相似度研究[J].化学分析计量,2006,15(6):54-55
[61]李玉锋,颜钫,唐琳等.化学计量学方法应用于贝母化学特征指纹图谱的研究[J].中药材,2006,29(3):216-218
[62]吴广,谢柏艳,张勇慧等.不同环境对湖北贝母总生物碱含量与指纹图谱的影响[J].医药导报,2008,27(6):707-710
[63]赵晶,高玮,徐帆等.几种含金丝桃素植物提取物的HPLC分析[J].中国药学杂志,2003.38(10):750-752
[64]甄汉深,张三平,高岚.薄层扫描法测定乌贝散中贝母素甲、贝母素乙的含量[J].中成药,1997,19(11):13-14
[65]陈信义,张庆林,唐仲雄等.HPLC法测定市售贝母中贝母乙素含量[J].中草药,2001,32(1):24-25
[66]李松林,李萍,林鸽等..药用贝母中几种活性异甾体生物碱的分布[J].药学学报,1999,34(11):842-847
[67]洪梅,马或,李秀芬等.HPLC-ELSD测定平贝母中贝母素甲的含量[J].中国中药杂志,2006,31(12):1032-1033
[68]李萍,徐国钩,徐路珊.贝母中的贝母碱、去氢贝母碱含量测定[J].中草药,1993,24(11):5791
[69]薛燕,刘文殷.RP-HPLC法测定浙贝炮制品中主要生物碱的含量[J].药物分析杂志,2006,26(1):60
[70]薛燕,顾好粮.HPLC-ELSD法测定浙贝母中主要生物碱的含量[J].药学学报,2005,40(6):550
[71]夏德豪,程显隆,肖新月等.平贝母中总生物碱含量测定方法学研究[J].中国药事,2007,21(9):756-758
[72]宋福坤,赵勤,邢卫等.不同产地贝母总生物碱含量的比较[J].华西药学杂志,1997,12(4):259-261
[73]刘军,谢锦桃,吴忠.贝母总生物碱及微量元素的研究[J].广东微量元素科学,1995,2(9):63-64
[74]王曙,徐小平,李涛.川贝母与其他贝母类药材总生物碱和总皂苷的含量测定与比较[J].中国中药杂志,2002,27(5):342-344
[75]董光平,马晓匡.云南省产贝母中总生物碱的含量测定[J].大理医学院学报,1998,7(3):11
[76]李娟,张鹏,张国欣等.息咳胶囊中湖北贝母总生物碱含量测定方法的研究[J].中国中药杂志,2005,30(12):947-948
[77]王艳红,吴晓民,郑友兰.不同产地和采收期的平贝母总生物碱含量[J].中药材,2006,29(1):8-9
[78]宋福坤,赵勤.湖北贝母与川贝母总生物碱含量的比较[J].药学进展,1998,22(1):49-50
[79]邹冈.基础神经药理学[M].北京:科学出版社,1988:310
[80]郭力,梁恒兴,赵斌等.板蓝根和大青叶类药材腺苷含量的测定[J].中成药,2006,28(7):1064-1066
[81]郑晓珂,冯卫生,毕跃峰等.RP-HPLC法测定卷柏中腺苷的含量[J].上海中医药大学学报,2003,17(2):42-44
[82]许润春,杨明,苏艳桃.HPLC测定板蓝根中腺苷含量[J].中成药,2005,27(6):742-743
[83]吴晓民,王艳红,郑友兰.HPLC测定不同产地平贝母中腺苷的含量[J].华西药学杂志,2006,21(1):074-076
[2]朱喜,注.诗经.上海:上海古籍出版社,1988.23
[3]郝懿行.尔雅义疏(下一).北京市中国书店铺:释草24
[4]赵学敏.本草纲目拾遗[M].第2版.人民卫生出版社,1983.123
[5]谢志民,王敏春,吕润霞.贝母类中药品种的本草考证[J].中药材,2000,23(7).
[6]谢宗万.中药材品种论述[M].第2版(上册).上海科学技术出版社,1990.388
[7]张建兴.浙贝母新鲜鳞茎化学成分的研究[J].中国中药杂志,1993,18(6):354-355
[8]马鸿年.川贝母商品松贝和青贝的生物碱含量[J].中国中药杂志,1995,20(3)
[9]刘庆华.新疆贝母化学成分研究[J].药学学报,1984,19(12):894
[10]徐东铭.伊贝母生物碱的研究[J].植物学报,1990,32
[11]徐东铭,张本,祁彦.平贝母生物碱研究Ⅱ[J].中草药,1983,14(2):7-8
[12]李兴斌,高燕飞,李吉良.平贝母化学成分及药理活性研究进展[J].中医药信息,2004.21(4)
[13]Kitamura:Y,et,al.Tetraberdron Lett.1988,45(18):5755-5758
[14]Kitamura:Y,et,al.Tennen Yukikagobustsu Toronkaikoen Yashishu.1989,31(52):6829-6832
[15]Kitamura:Y,et,al.Tetraberdron Lett.1989,30(3):4981-4984
[16]Kitamura:Y,et,al.Tetraberdron Lett.1989,45(23):7281-7283
[17]CA1990,112:14555LW
[18]肖培根.湖北贝母的研究进展[J].中国中药杂志,2002,27(10):726
[19]Kitajima J,Komori T,Kawasaki T,et al.Studies on the Constituents of Crude Drug "Fritillariae Bulbus"[J].Chem Pharm Bull,1982,30:3912-3921
[20]Kitajima J,Noda T N,Ida Y,et al.Studies on the Constituents of Crude Drug "Fritillariae Bulbus"Ⅳ[J].Chem Pharm Bull,1982,30:3922-3931
[21]北岛润一,古森微哉,川珐敏男.Studies on the Constituents of Crude Drug "Fritillariae
[22]北岛润一,伊田喜光,野田直希,等.Studies on the constituents of Crude Drug "Fritillariae Bulbus"Ⅵ[J].药学杂志,1982,102:1016-1022
[23]陈泽乃,陆阳,徐佩娟等.中药贝母中水溶性成分的研究[J].中国中药杂志,1996,21 (7):420-424
[24]沙世炎.中草药有效成分分析法[M](上册).北京:人民卫生出版社,1982.59
[25]李萍,曾令杰,李松林.无紫外吸收的贝母总生物碱定量分析方法研究[J].中国药学杂志,2002,37(8)
[26]王冲之,孙健,李萍.贝母类药材生物碱及生物碱苷含量测定方法学研究[J].中国药学杂志,2003,38(6)
[27]徐汝明,刘海卫,陆阳等.双波长紫外分光光度法测定贝母中腺苷和胸苷的含量[J].药学学报,1997,32(8):617-619
[28]薛燕,王峰.不同产地浙贝母药材中3种活性成分的分析研究[J].中国中药杂志,2007,32(16):1628-1630
[29]闫明,仲婕,刘庆华等.十种贝母裂解气相色谱分析鉴定[J].中国现代应用药学,1996,13(5):19-21
[30]张立群,熊俭,付秉昌..双波长薄层扫描法测定湖北贝母中湖贝甲素的含量[J].中国药师,1999,2(3):143-144
[31]张鹏,徐仿周.贝母及其复方制剂中生物碱含量测定方法[J].医药导报.2004,23(4):262
[32]姜艳,李萍.HPLC-ELSD测定浙贝母中贝母素甲、贝母素乙的含量[J].中国药学杂志,2005,40(16):1259
[33]谢志民,吕润霞.贝母类中药功效订正[J].中药材,2001,24(8):600-602
[34]于晓琳,季晖,王长礼等.贝母的药理作用研究概况[J].中草药,2000,31(4):313-314
[35]姚丽娜,孙汉清,江湛等.湖北贝母、鄂北贝母、紫花鄂北贝母总碱对呼吸系统的药理作用[J].同济医科大学学报,1993,22(1):47-49
[36]Chen K K,et al1.Chinese J Physiology.1935,9(1):21
[37]Siitsul K,et al1.JPn1 Kokai Tokkvo Koho JP 62,164,622[87,164,622]
[38]silsu K,et al1.生药学杂志(日).1987,41(3):174
[39]张勇慧,阮汉利,吴继洲.贝母的药理作用研究概况[J].医药导报,2003,22(11)
[40]张鹏,皮慧芳,张久亮等.湖北贝母茎叶中的生物碱类成分研究[J].中草药,2008,39(9):1294-1296
[41]张久亮,皮慧芳,阮汉利等.湖北贝母茎叶非生物碱成分的研究[J].医药导报,2007,26(1):23-25
[42]汪殿蓓,暨淑仪,陈飞鹏.植物群落物种多样性研究综述[J].生态学杂志,2001,20(4):55
[43]贺金生,马克平.物种多样性[A].见:蒋志刚.保护生物学[C].杭州:杭州科学技术出版社,1997.20-33
[44]林长松,左经会,廖雯.稀有植物十齿花群落物种多样性研究[J].植物研究,2008,28(3)
[45]余世春,肖培根.贝母属植物异甾体生物碱的存在及其分类学意义[J].植物分类学报,1992,30(5):450
[46]中国科学院中国植物志编辑委员会.《中国植物志》.北京:科学出版社,1988
[47]中国药典[S].2005年版.一部
[48]王天志,杜蕾蕾.川贝母的研究进展[J].华西药学杂志,2001,16(3):200
[49]李萍,徐国钧.中药贝母类的资源利用研究[J].植物资源与环境,1993,2(3)12-17
[50]何斜.中药贝母的品种与性状区别[J].海峡药学,2002,14(6):55
[51]唐进,汪发缵.中国植物志,十四卷.北京:科学出版社,1980.97-116
[52]陈心放,余国奠,周印锁.甘肃贝母属新植物[J].云南植物研究,1985,7(2):146-150
[53]杨永康,吴家坤.国产贝母属的新分类群[J].西北植物学报,1985,5(1):26-40
[54]张耀甲,程林.甘肃贝母属植物分类学研究[J].兰州大学学报(自然科学版),1998,34(2):84-85
[55]张恩和,黄高宝,景锐.西北药用植物资源多样性保护与持续利用[J].甘肃科学学报,1997,9(1):43-44
[56]庄起明,马骥,李俊祯.甘肃药用植物资源多样性及其保护利用[J].中国野生植物资源,2003,22(1):11-13
[57]刘丽莎.药用植物遗传多样性的保护和利用[J].甘肃中医学院学报,1999,(3):60-62
[58]马克平,钱迎倩.生物多样性保护及其研究进展[J].应用与环境生物学报,1998,4(1):95-99
[59]王永刚,吴忠,魏凤环等.中药指纹图谱研究的现状与未来[J].中药材,2003,26(11):823-825
[60]孙庆雷,林云良,祝贺等.HPLC指纹图谱相似度研究[J].化学分析计量,2006,15(6):54-55
[61]李玉锋,颜钫,唐琳等.化学计量学方法应用于贝母化学特征指纹图谱的研究[J].中药材,2006,29(3):216-218
[62]吴广,谢柏艳,张勇慧等.不同环境对湖北贝母总生物碱含量与指纹图谱的影响[J].医药导报,2008,27(6):707-710
[63]赵晶,高玮,徐帆等.几种含金丝桃素植物提取物的HPLC分析[J].中国药学杂志,2003.38(10):750-752
[64]甄汉深,张三平,高岚.薄层扫描法测定乌贝散中贝母素甲、贝母素乙的含量[J].中成药,1997,19(11):13-14
[65]陈信义,张庆林,唐仲雄等.HPLC法测定市售贝母中贝母乙素含量[J].中草药,2001,32(1):24-25
[66]李松林,李萍,林鸽等..药用贝母中几种活性异甾体生物碱的分布[J].药学学报,1999,34(11):842-847
[67]洪梅,马或,李秀芬等.HPLC-ELSD测定平贝母中贝母素甲的含量[J].中国中药杂志,2006,31(12):1032-1033
[68]李萍,徐国钩,徐路珊.贝母中的贝母碱、去氢贝母碱含量测定[J].中草药,1993,24(11):5791
[69]薛燕,刘文殷.RP-HPLC法测定浙贝炮制品中主要生物碱的含量[J].药物分析杂志,2006,26(1):60
[70]薛燕,顾好粮.HPLC-ELSD法测定浙贝母中主要生物碱的含量[J].药学学报,2005,40(6):550
[71]夏德豪,程显隆,肖新月等.平贝母中总生物碱含量测定方法学研究[J].中国药事,2007,21(9):756-758
[72]宋福坤,赵勤,邢卫等.不同产地贝母总生物碱含量的比较[J].华西药学杂志,1997,12(4):259-261
[73]刘军,谢锦桃,吴忠.贝母总生物碱及微量元素的研究[J].广东微量元素科学,1995,2(9):63-64
[74]王曙,徐小平,李涛.川贝母与其他贝母类药材总生物碱和总皂苷的含量测定与比较[J].中国中药杂志,2002,27(5):342-344
[75]董光平,马晓匡.云南省产贝母中总生物碱的含量测定[J].大理医学院学报,1998,7(3):11
[76]李娟,张鹏,张国欣等.息咳胶囊中湖北贝母总生物碱含量测定方法的研究[J].中国中药杂志,2005,30(12):947-948
[77]王艳红,吴晓民,郑友兰.不同产地和采收期的平贝母总生物碱含量[J].中药材,2006,29(1):8-9
[78]宋福坤,赵勤.湖北贝母与川贝母总生物碱含量的比较[J].药学进展,1998,22(1):49-50
[79]邹冈.基础神经药理学[M].北京:科学出版社,1988:310
[80]郭力,梁恒兴,赵斌等.板蓝根和大青叶类药材腺苷含量的测定[J].中成药,2006,28(7):1064-1066
[81]郑晓珂,冯卫生,毕跃峰等.RP-HPLC法测定卷柏中腺苷的含量[J].上海中医药大学学报,2003,17(2):42-44
[82]许润春,杨明,苏艳桃.HPLC测定板蓝根中腺苷含量[J].中成药,2005,27(6):742-743
[83]吴晓民,王艳红,郑友兰.HPLC测定不同产地平贝母中腺苷的含量[J].华西药学杂志,2006,21(1):074-076
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |