低剂量辐射对IL-6和SOD的影响及其适应性反应的研究
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摘要
目的 探讨低剂量辐射对IL—6、SOD、白蛋白、球蛋白的刺激作用、适应性反应。
方法 采用ICR小鼠，分10组，每组12只。用~(60)Co γ射线对其中1至8组分别照射7.5、10、12.5、20、30、100、200、300cGy，用以观察刺激作用；第9组小鼠接受300cGy照射前6小时，给予7.5 cGy预照射，观察适应性反应；另设1个对照组。照射后24小时采血，用放免法测定IL—6、SOD，用分光光度法测定蛋白。
结果 实验设定条件下，对白蛋白、球蛋白尚未看到低剂量辐射的刺激作用。低剂量辐射对SOD的刺激作用发生在12.5cGy。发现在7.5到30cGy间，低剂量辐射对IL—6的刺激作用，存在多个剂量点；7.5 cGy能诱导IL-6的适应性反应，受照剂量300 cGy时，IL—6明显升高，且幅度很大。
结论 低剂量辐射对SOD、IL—6有刺激作用，且7.5 cGy的低剂量照射能诱导IL-6的适应性反应。白蛋白、球蛋白则无类似响应。7.5到30 cGy间存在多个诱导刺激作用的剂量点，提示导致IL-6刺激作用的剂量范围可能较宽。较大剂量照射时，IL—6可能过度表达，机理有待进一步探讨。
Objective To research whether IL-6. SOD, immunoglobulin and albumin have the adaptive response and stimulation effect to low dose radiation or not.
Method ICR mouse is choiced as experimental animal and each group has 12 mice.8 groups of mouse was given different dose 60Co Y radiation to survey the hormesis and the radiation dose is 7.5, 10, 12.5, 20 , 30, 100, 200, 300 cGy respectively; the ninth group of mouse was given 7.5cGy beforehand radiation before 6 hours of 300 cGy radiation to survey the adaptive response. One control group was established. Blood samples were collected after 24 hours of radiation.IL-6 and SOD was measured by radioimmunoassay, immunoglobulin and albumin was test by spectrophotometer.
Results In the situation of the experiment, stimulation effect haven't been found in immunoglobulin and albumin; stimulation effect of low dose radiation to SOD was survey in the dose of 12.5 cGy; we also found that there is several dose point from 7.5 to 30 cGy have stimulation effect to IL-6,meantime and radiation of 7.5 cGy have adaptive response to IL-6; IL-6 increased drastically in the group of 300 cGy of radiation .
Conclusion Low dose radiation have stimulation effect to IL-6 and SOD and radiation of 7.5 cGy can result in the adaptive response of IL-6,whereas this phenomenon has not been survey in immunoglobulin and albumin.The fact that different dose have stimulation effect to IL-6
indicated that the range of stimulation dose to IL-6 is wide. Increase of IL-6 after high dose radiation maybe is result of excessive synthesis and the reason is worthy of more research.
方法 采用ICR小鼠，分10组，每组12只。用~(60)Co γ射线对其中1至8组分别照射7.5、10、12.5、20、30、100、200、300cGy，用以观察刺激作用；第9组小鼠接受300cGy照射前6小时，给予7.5 cGy预照射，观察适应性反应；另设1个对照组。照射后24小时采血，用放免法测定IL—6、SOD，用分光光度法测定蛋白。
结果 实验设定条件下，对白蛋白、球蛋白尚未看到低剂量辐射的刺激作用。低剂量辐射对SOD的刺激作用发生在12.5cGy。发现在7.5到30cGy间，低剂量辐射对IL—6的刺激作用，存在多个剂量点；7.5 cGy能诱导IL-6的适应性反应，受照剂量300 cGy时，IL—6明显升高，且幅度很大。
结论 低剂量辐射对SOD、IL—6有刺激作用，且7.5 cGy的低剂量照射能诱导IL-6的适应性反应。白蛋白、球蛋白则无类似响应。7.5到30 cGy间存在多个诱导刺激作用的剂量点，提示导致IL-6刺激作用的剂量范围可能较宽。较大剂量照射时，IL—6可能过度表达，机理有待进一步探讨。
Objective To research whether IL-6. SOD, immunoglobulin and albumin have the adaptive response and stimulation effect to low dose radiation or not.
Method ICR mouse is choiced as experimental animal and each group has 12 mice.8 groups of mouse was given different dose 60Co Y radiation to survey the hormesis and the radiation dose is 7.5, 10, 12.5, 20 , 30, 100, 200, 300 cGy respectively; the ninth group of mouse was given 7.5cGy beforehand radiation before 6 hours of 300 cGy radiation to survey the adaptive response. One control group was established. Blood samples were collected after 24 hours of radiation.IL-6 and SOD was measured by radioimmunoassay, immunoglobulin and albumin was test by spectrophotometer.
Results In the situation of the experiment, stimulation effect haven't been found in immunoglobulin and albumin; stimulation effect of low dose radiation to SOD was survey in the dose of 12.5 cGy; we also found that there is several dose point from 7.5 to 30 cGy have stimulation effect to IL-6,meantime and radiation of 7.5 cGy have adaptive response to IL-6; IL-6 increased drastically in the group of 300 cGy of radiation .
Conclusion Low dose radiation have stimulation effect to IL-6 and SOD and radiation of 7.5 cGy can result in the adaptive response of IL-6,whereas this phenomenon has not been survey in immunoglobulin and albumin.The fact that different dose have stimulation effect to IL-6
indicated that the range of stimulation dose to IL-6 is wide. Increase of IL-6 after high dose radiation maybe is result of excessive synthesis and the reason is worthy of more research.
引文
1. Olivieri G,Bodyncote J.Wolf S.Adaptive response of human lymphocytes to low concentration of radioactive thymidine. Science, 1984, 23:594-595.
2. Liu SZ,Cai L,Sun SQ.Induction of a cytogenietic adaptive response by exposure of rabbits to very low dose-rate γ-radiation. Int J Radiat Boi, 1992,62:187-190.
3．刘树铮．低剂量辐射诱导适应性反应的普遍性．中华放射医学与防护杂志，1995，15：293．
4．陈沙力，孟庆勇，刘树峥．低剂量辐射诱导小鼠胸腺细胞蛋白的早期表达．中华放射医学与防护杂志，1996，16(3)：161～163．
5．栗原涛之．极低量放射的生物效应[J]．放射科学，1991，34(6)：171．
6 蔡露．低剂量辐射的兴奋效应及其诱导的适应性反应[J]．国外医学放射医学核医学分册，1991，15(6)：241．
7 王献理，苏士杰，尹洪淑，等．低剂量辐射对小鼠种植肿瘤及其放疗的影响[J]．中华放射医学与防护杂志，1996，16(3)：157-160．
8 范雪云，李君，白玉萍，等．放射人员染色体损伤与血清SOD活性和细胞免疫功能的关系[J]．中华放射医学与防护杂志，1997，17(5)：318．
9 宋墨琴，卢林，陈晖，等．SOD及双歧杆菌抗辐射的研究[J]．福建医药杂志，1999，21(4)：87．
10 孙世荃．人类辐射危害评价[M]．北京：原子能出版社，1996．58-62．
11 于洪升，卢彦达．低剂量电离辐射对小鼠种植瘤的生长及红细胞免疫SOD活性的影响[J]．中华放射医学与防护杂志，2001，21(5)：354-357．
2. Liu SZ,Cai L,Sun SQ.Induction of a cytogenietic adaptive response by exposure of rabbits to very low dose-rate γ-radiation. Int J Radiat Boi, 1992,62:187-190.
3．刘树铮．低剂量辐射诱导适应性反应的普遍性．中华放射医学与防护杂志，1995，15：293．
4．陈沙力，孟庆勇，刘树峥．低剂量辐射诱导小鼠胸腺细胞蛋白的早期表达．中华放射医学与防护杂志，1996，16(3)：161～163．
5．栗原涛之．极低量放射的生物效应[J]．放射科学，1991，34(6)：171．
6 蔡露．低剂量辐射的兴奋效应及其诱导的适应性反应[J]．国外医学放射医学核医学分册，1991，15(6)：241．
7 王献理，苏士杰，尹洪淑，等．低剂量辐射对小鼠种植肿瘤及其放疗的影响[J]．中华放射医学与防护杂志，1996，16(3)：157-160．
8 范雪云，李君，白玉萍，等．放射人员染色体损伤与血清SOD活性和细胞免疫功能的关系[J]．中华放射医学与防护杂志，1997，17(5)：318．
9 宋墨琴，卢林，陈晖，等．SOD及双歧杆菌抗辐射的研究[J]．福建医药杂志，1999，21(4)：87．
10 孙世荃．人类辐射危害评价[M]．北京：原子能出版社，1996．58-62．
11 于洪升，卢彦达．低剂量电离辐射对小鼠种植瘤的生长及红细胞免疫SOD活性的影响[J]．中华放射医学与防护杂志，2001，21(5)：354-357．