对虾MyD88和STAT在WSSV感染过程中的功能研究
摘要
对虾是重要的水产养殖种类之一，由于各类病害的爆发，特别是白斑综合症的威胁，对虾养殖产业经受了巨大的损失。对虾抵御病原侵袭的方式是启动先天免疫应答系统，特别是通过信号通路的启动完成对病原的识别和清除。已有研究表明，对虾先天免疫系统中两条重要的信号通路：Toll通路和JAK/STAT通路可能参与了对虾应答白斑综合症病毒的过程。然而，有关对虾Toll通路的关键接头分子MyD88的功能尚未报道，JAK/STAT通路的转录因子STAT在对虾感染WSSV过程中的功能尚不清楚。基于上述问题，本文对对虾的MyD88和STAT在WSSV侵染过程中的作用进行了研究，研究进展如下：
1.从中国明对虾中克隆获得了MyD88的同源基因---FcMyD88，其cDNA全长1434bp，编码477个氨基酸。推导的氨基酸序列中包含死亡结构域（deathdomain，DD）和TIR结构域（typical TLR and IL-1R-related，TIR），在其C端有一段约100个氨基酸组成的C端延伸区域（C-terminal extension，CTE）。定量PCR和免疫印迹分析发现，FcMyD88mRNA在藤黄微球菌（Micrococcus luteus）和鳗弧菌（Vibrio anguillarum）刺激后的24小时内均上调表达；FcMyD88蛋白在V. anguillarum刺激的12小时上调表达，而在M. luteus刺激后无明显变化；FcMyD88mRNA虽然在WSSV刺激后表达量相对稳定，但是FcMyD88蛋白在WSSV刺激后明显上调表达；用WSSV感染MyD88沉默后的对虾，由WSSV感染引起的对虾死亡率显著降低。以上结果表明MyD88依赖的信号通路参与了对虾抵御细菌和WSSV感染过程，MyD88可能有利于WSSV的感染。
2.利用dsRNA干扰技术沉默对虾STAT基因后再感染WSSV，观察WSSV在对虾中的拷贝数和由WSSV感染引起的对虾死亡率，结果表明，在STAT基因沉默后的对虾中，WSSV拷贝数维持在102-103copies/ng DNA，显著少于对照组，并且其死亡率也显著低于对照组。为进一步了解STAT基因的功能，又利用培养的螯虾造血细胞进行了研究，利用STAT3特异抑制剂（S3I-201）抑制鳌虾造血细胞的STAT活性后再用WSSV感染细胞，其细胞存活率显著高于未经抑制剂处理的对照组，说明STAT在WSSV感染对虾的过程中起着重要作用。为了解在STAT干扰后的对虾中STAT通路可能调控的目的基因以及RNAi调控通路的相关基因的表达，利用实时定量PCR技术对STAT干扰后的对虾中相关基因的表达进行了分析，结果表明，与注射EGFP双链RNA的对照组相比，在STAT被干扰的对虾中再感染WSSV后，GILT, Dicer2和AGO2基因的表达明显上调，而AGO1和Dicer1基因的表达没有发生明显变化，说明STAT被干扰后的对虾可能通过增强siRNA通路达到抗WSSV感染的目的。
3.利用高通量的RNA-seq技术筛选STAT被干扰的对虾中差异表达的基因，结果表明，STAT干扰引起了参与能量代谢、糖代谢、外骨骼组成、转录及免疫等生命过程的基因表达的变化。其中，与能量代谢相关酶类，如腺苷酸环化酶、细胞色素c氧化酶I亚基；外骨骼组成相关蛋白，如Culticle、DD5、DD9A；转录相关分子，如转录起始因子TFIID和RNA解旋酶等基因的表达均下调。而与糖代谢相关分子、免疫相关分子等的表达既有上调也有下调，说明STAT干扰改变了对虾体内许多基因的转录表达，这些基因的表达变化可能不利于WSSV在对虾体内的复制。本研究筛选的对虾STAT潜在调控的基因信息，为进一步分析JAK/STAT对虾免疫中的功能奠定了重要基础。
Shrimp is the one of the major species in aquaculture. Disease is always aproblem which hinders the healthy development of shrimp aquaculture. Both virusand bacteria are dangerous pathogens to shrimp aquaculture. Shrimp defense againstpathogens mainly by initiating the innate immunue signal pathways. Toll andJAK/STAT signaling pathways play key roles in the antiviral immunity of mammals,fish and insect. However, limited knowledge is known about the function of MyD88and STAT in shrimp, which are regarded as an key component of Toll or JAK/STATsignaling pathway respectively, during WSSV infection. The present thesis mainlyfocused on the function of MyD88and STAT in Penaeid shrimp. The main progressesare as follows:
1. The full length cDNA of a MyD88homologue (FcMyD88) was cloned frompenaeid shrimp Fenneropenaeus chinensis. The ORF of FcMyD88consisted of1434bp encoding477amino acids, which contains a death domain (DD), a typical TLRand IL-1R-related (TIR) domain and a C-terminal extension (CTE). Homologyanalysis revealed that the deduced amino acid sequence of FcMyD88shared morethan47%similarities with a variety of previously reported MyD88s. Thetime-dependent expression patterns of FcMyD88in cephalothoraxes of shrimpsinjected by heat killed Vibrio anguillarum (G-), Micrococcus luteus (G+) and livewhite syndrome spot virus (WSSV) were analyzed at transcription and protein levelby qPCR and Western Blot, respectively. The expression of FcMyD88mRNA wassignificantly up-regulated during24h after injection with both V. anguillarum and M.luteus. The expression level of FcMyD88protein was up-regulated at12hour postinjection (hpi) of V. anguillarum, while it didn’t change after M. lysodeikticu injectionfor12h. After WSSV injection, the expression level of FcMyD88mRNA wasrelatively constant, while the FcMyD88protein level was significantly up-regulated at12and24hpi. Further, dsRNA interfering technique was used to silence the expression of MyD88in Litopenaeus vannamei, and the mortality of shrimp wasreduced significantly compared to their controls during WSSV infection. These resultssuggested that the MyD88-dependent signaling pathway could be involved in thedefense of shrimp both to bacteria and WSSV infection, and shrimp MyD88mightbenefit to WSSV infection.
2. In order to understand the function of JAK/STAT signaling pathway in theantiviral immunity of shrimp, dsRNA interfering technique was used to silence theexpression of STAT gene in Litopenaeus vannamei, and the mortality of shrimp wasdetected after WSSV infection. The WSSV copy number in STAT silenced shrimp was102-103copies/ng DNA which was much lower than those in the control and themortality in STAT silenced shrimp caused by WSSV infection reduced verysignificantly compared to their controls. The function of STAT was verified in vitrocultured cells of hematopoietic tissue of crayfish Cherax quadricarinatus by addingspecific inhibitor of STAT3(S3I-201), and the cultured cells treated with S3I-201showed much less WSSV copy number than their controls, which further suggestedthat STAT might be helpful for the replication of WSSV. Furthermore, the expressionprofile of some potential target genes regulated by STAT or genes related to RNAinterfering pathway were detected in STAT silenced shrimp during WSSV infection.Dicer2and AGO2were up-regulated in STAT silenced shrimp, which implied thatsilencing of STAT might enhance the expression of siRNA pathway related genes toresist the WSSV infection.
3. The differentially expressed genes (DEGs) in STAT silenced shrimp wasscreened by high throughput RNA-Seq. The data showed that silencing of STATcaused transcription variations of genes related to energy metabolism, glucosemetabolism, composition of the exoskeleton, immune response and transcription.Among the DEGs, genes encoding enzymes related to energy metabolism such asadenylate cyclase and cytochrome c oxidase subunit I, genes encoding proteins relatedto exoskeleton compositions, such as cuticle protein, DD5and DD9A, and genesencoding transcription related factors such as ATP-dependent RNA helicase A,,transcription initiation factor TFIID etc. showed down-regulation. Genes related to glucose metabolism and immunity showed up or down regulations, which suggestedthat interference of STAT changed the expression of a lot of genes. The variations ofthese genes’ expression might not be benefical to the replication of WSSV in shrimp.,These information about the potential genes regualted by STAT will help us tounderstand the function of JAK/STAT pathway in the immunity of shrimp.
1.从中国明对虾中克隆获得了MyD88的同源基因---FcMyD88，其cDNA全长1434bp，编码477个氨基酸。推导的氨基酸序列中包含死亡结构域（deathdomain，DD）和TIR结构域（typical TLR and IL-1R-related，TIR），在其C端有一段约100个氨基酸组成的C端延伸区域（C-terminal extension，CTE）。定量PCR和免疫印迹分析发现，FcMyD88mRNA在藤黄微球菌（Micrococcus luteus）和鳗弧菌（Vibrio anguillarum）刺激后的24小时内均上调表达；FcMyD88蛋白在V. anguillarum刺激的12小时上调表达，而在M. luteus刺激后无明显变化；FcMyD88mRNA虽然在WSSV刺激后表达量相对稳定，但是FcMyD88蛋白在WSSV刺激后明显上调表达；用WSSV感染MyD88沉默后的对虾，由WSSV感染引起的对虾死亡率显著降低。以上结果表明MyD88依赖的信号通路参与了对虾抵御细菌和WSSV感染过程，MyD88可能有利于WSSV的感染。
2.利用dsRNA干扰技术沉默对虾STAT基因后再感染WSSV，观察WSSV在对虾中的拷贝数和由WSSV感染引起的对虾死亡率，结果表明，在STAT基因沉默后的对虾中，WSSV拷贝数维持在102-103copies/ng DNA，显著少于对照组，并且其死亡率也显著低于对照组。为进一步了解STAT基因的功能，又利用培养的螯虾造血细胞进行了研究，利用STAT3特异抑制剂（S3I-201）抑制鳌虾造血细胞的STAT活性后再用WSSV感染细胞，其细胞存活率显著高于未经抑制剂处理的对照组，说明STAT在WSSV感染对虾的过程中起着重要作用。为了解在STAT干扰后的对虾中STAT通路可能调控的目的基因以及RNAi调控通路的相关基因的表达，利用实时定量PCR技术对STAT干扰后的对虾中相关基因的表达进行了分析，结果表明，与注射EGFP双链RNA的对照组相比，在STAT被干扰的对虾中再感染WSSV后，GILT, Dicer2和AGO2基因的表达明显上调，而AGO1和Dicer1基因的表达没有发生明显变化，说明STAT被干扰后的对虾可能通过增强siRNA通路达到抗WSSV感染的目的。
3.利用高通量的RNA-seq技术筛选STAT被干扰的对虾中差异表达的基因，结果表明，STAT干扰引起了参与能量代谢、糖代谢、外骨骼组成、转录及免疫等生命过程的基因表达的变化。其中，与能量代谢相关酶类，如腺苷酸环化酶、细胞色素c氧化酶I亚基；外骨骼组成相关蛋白，如Culticle、DD5、DD9A；转录相关分子，如转录起始因子TFIID和RNA解旋酶等基因的表达均下调。而与糖代谢相关分子、免疫相关分子等的表达既有上调也有下调，说明STAT干扰改变了对虾体内许多基因的转录表达，这些基因的表达变化可能不利于WSSV在对虾体内的复制。本研究筛选的对虾STAT潜在调控的基因信息，为进一步分析JAK/STAT对虾免疫中的功能奠定了重要基础。
Shrimp is the one of the major species in aquaculture. Disease is always aproblem which hinders the healthy development of shrimp aquaculture. Both virusand bacteria are dangerous pathogens to shrimp aquaculture. Shrimp defense againstpathogens mainly by initiating the innate immunue signal pathways. Toll andJAK/STAT signaling pathways play key roles in the antiviral immunity of mammals,fish and insect. However, limited knowledge is known about the function of MyD88and STAT in shrimp, which are regarded as an key component of Toll or JAK/STATsignaling pathway respectively, during WSSV infection. The present thesis mainlyfocused on the function of MyD88and STAT in Penaeid shrimp. The main progressesare as follows:
1. The full length cDNA of a MyD88homologue (FcMyD88) was cloned frompenaeid shrimp Fenneropenaeus chinensis. The ORF of FcMyD88consisted of1434bp encoding477amino acids, which contains a death domain (DD), a typical TLRand IL-1R-related (TIR) domain and a C-terminal extension (CTE). Homologyanalysis revealed that the deduced amino acid sequence of FcMyD88shared morethan47%similarities with a variety of previously reported MyD88s. Thetime-dependent expression patterns of FcMyD88in cephalothoraxes of shrimpsinjected by heat killed Vibrio anguillarum (G-), Micrococcus luteus (G+) and livewhite syndrome spot virus (WSSV) were analyzed at transcription and protein levelby qPCR and Western Blot, respectively. The expression of FcMyD88mRNA wassignificantly up-regulated during24h after injection with both V. anguillarum and M.luteus. The expression level of FcMyD88protein was up-regulated at12hour postinjection (hpi) of V. anguillarum, while it didn’t change after M. lysodeikticu injectionfor12h. After WSSV injection, the expression level of FcMyD88mRNA wasrelatively constant, while the FcMyD88protein level was significantly up-regulated at12and24hpi. Further, dsRNA interfering technique was used to silence the expression of MyD88in Litopenaeus vannamei, and the mortality of shrimp wasreduced significantly compared to their controls during WSSV infection. These resultssuggested that the MyD88-dependent signaling pathway could be involved in thedefense of shrimp both to bacteria and WSSV infection, and shrimp MyD88mightbenefit to WSSV infection.
2. In order to understand the function of JAK/STAT signaling pathway in theantiviral immunity of shrimp, dsRNA interfering technique was used to silence theexpression of STAT gene in Litopenaeus vannamei, and the mortality of shrimp wasdetected after WSSV infection. The WSSV copy number in STAT silenced shrimp was102-103copies/ng DNA which was much lower than those in the control and themortality in STAT silenced shrimp caused by WSSV infection reduced verysignificantly compared to their controls. The function of STAT was verified in vitrocultured cells of hematopoietic tissue of crayfish Cherax quadricarinatus by addingspecific inhibitor of STAT3(S3I-201), and the cultured cells treated with S3I-201showed much less WSSV copy number than their controls, which further suggestedthat STAT might be helpful for the replication of WSSV. Furthermore, the expressionprofile of some potential target genes regulated by STAT or genes related to RNAinterfering pathway were detected in STAT silenced shrimp during WSSV infection.Dicer2and AGO2were up-regulated in STAT silenced shrimp, which implied thatsilencing of STAT might enhance the expression of siRNA pathway related genes toresist the WSSV infection.
3. The differentially expressed genes (DEGs) in STAT silenced shrimp wasscreened by high throughput RNA-Seq. The data showed that silencing of STATcaused transcription variations of genes related to energy metabolism, glucosemetabolism, composition of the exoskeleton, immune response and transcription.Among the DEGs, genes encoding enzymes related to energy metabolism such asadenylate cyclase and cytochrome c oxidase subunit I, genes encoding proteins relatedto exoskeleton compositions, such as cuticle protein, DD5and DD9A, and genesencoding transcription related factors such as ATP-dependent RNA helicase A,,transcription initiation factor TFIID etc. showed down-regulation. Genes related to glucose metabolism and immunity showed up or down regulations, which suggestedthat interference of STAT changed the expression of a lot of genes. The variations ofthese genes’ expression might not be benefical to the replication of WSSV in shrimp.,These information about the potential genes regualted by STAT will help us tounderstand the function of JAK/STAT pathway in the immunity of shrimp.
引文
AGAISSE, H., and N. PERRIMON,2004The roles of JAK/STAT signaling in Drosophila immuneresponses. Immunological reviews198:72-82.
AKIRA, S., K. TAKEDA and T. KAISHO,2001Toll-like receptors: critical proteins linking innate andacquired immunity. Nature immunology2:675-680.
AYYAZ, A., P. GIAMMARINARO, S. LIEGEOIS, M. LESTRADET and D. FERRANDON,2013A negative rolefor MyD88in the resistance to starvation as revealed in an intestinal infection of Drosophilamelanogaster with the Gram-positive bacterium Staphylococcus xylosus. Immunobiology218:635-644.
BARTON, G. M., and J. C. KAGAN,2009A cell biological view of Toll-like receptor function: regulationthrough compartmentalization. Nature reviews immunology9:535-542.
BASU, M., B. SWAIN, B. R. SAHOO, N. K. MAITI and M. SAMANTA,2012Induction of toll-like receptor(TLR)2, and MyD88-dependent TLR-signaling in response to ligand stimulation and bacterialinfections in the Indian major carp, mrigal (Cirrhinus mrigala). Molecular biology reports39:6015-6028.
BENEDICT, C. A., P. S. NORRIS and C. F. WARE,2002To kill or be killed: viral evasion of apoptosis.Nature immunology3:1013-1018.
BONILLA, C. M. E.,2011Application of RNA interference (RNAi) against viral infections in shrimp: areview. Journal of antivirals and antiretrovirals05.
BONNERT, T. P., K. E. GARKA, P. PARNET, G. SONODA, J. R. TESTA et al.,1997The cloning andcharacterization of human MyD88: A member of an IL-1receptor related family. FEBS letters402:81-84.
BURGE, E. J., D. J. MADIGAN, L. E. BURNETT and K. G. BURNETT,2007Lysozyme gene expression byhemocytes of Pacific white shrimp, Litopenaeus vannamei, after injection with Vibrio. Fish andshellfish immunology22:327-339.
CAIPANG, C. M. A., I. HIRONO and T. AOKI,2005Induction of antiviral state in fish cells by Japaneseflounder, Paralichthys olivaceus, interferon regulatory factor-1. Fish and shellfish immunology19:79-91.
CASANOVA, J. L., L. ABEL and L. QUINTANA-MURCI,2011Human TLRs and IL-1Rs in host defense:natural insights from evolutionary, epidemiological, and clinical genetics. Annual review ofimmunology29:447-491.
CHANG, P. S., C. F. LO, Y. C. WANG and G. H. KOU,1996Identification of white spot syndromeassociated baculovirus (WSBV) target organs in the shrimp Penaeus monodon by in situhybridization. Diseases of aquatic organisms27:131-139.
CHEN, I. T., T. AOKI, Y. T. HUANG, I. HIRONO, T. C. CHEN et al.,2011White Spot Syndrome Virusinduces metabolic changes resembling the Warburg effect in shrimp hemocytes in the early stageof infection. Journal of virology85:12919-12928.
CHEN, W. Y., K. C. HO, J. H. LEU, K. F. LIU, H. C. WANG et al.,2008WSSV infection activates STATin shrimp. Developmental and comparative immunology32:1142-1150.
COPELAND, N. G., D. J. GILBERT, C. SCHINDLER, Z. ZHONG, Z. WEN et al.,1995Distribution of themammalian stat gene family in mouse chromosomes. Genomics29:225-228.
CRONIN, J. G., M. L. TURNER, L. GOETZE, C. E. BRYANT and I. M. SHELDON,2011Toll-like receptor4and MyD88-dependent signaling mechanisms of the innate immune system are essential for theresponse to Lipopolysaccharide by epithelial and stromal cells of the bovine endometrium.Biology of reproduction86(2):1-9.
DARNELL, J. E.,1997STATs and gene regulation. Science277:1630-1635.
DAVID, S. A., and V. I. MATHAN,1993Enzyme-linked immunosorbent assay for quantification ofanti-lipid a antibody: enhancement of sensitivity by antigen immobilization at low pH. Journal ofimmunological methods163:85-89.
DE LA PE A, L. D., T. TAMAKI, K. MOMOYAMA, T. NAKAI and K. MUROGA,1993Characteristics of thecausative bacterium of vibriosis in the kuruma prawn, Penaeus japonicus. Aquaculture115:1-12.
DE LA VEGA, E., N. A. O'LEARY, J. E. SHOCKEY, J. ROBALINO, C. PAYNE et al.,2008Anti-lipopolysaccharide factor in Litopenaeus vannamei (LvALF): a broad spectrum antimicrobialpeptide essential for shrimp immunity against bacterial and fungal infection. Molecularimmunology45:1916-1925.
DE ZOYSA, M., S. JUNG and J. LEE,2009First molluscan TNF-alpha homologue of the TNFsuperfamily in disk abalone: molecular characterization and expression analysis. Fish&shellfishimmunology26:625-631.
DING, S. W., and O. VOINNET,2007Antiviral immunity directed by small RNAs. Cell130:413-426.
DOSTERT, C., E. JOUANGUY, P. IRVING, L. TROXLER, D. GALIANA-ARNOUX et al.,2005The JAK-STATsignaling pathway is required but not sufficient for the antiviral response of Drosophila. Natureimmunology6:946-953.
EDITORS, T. D. M. M.,2012Jak-Stat Signaling: from basics to disease. Springer.
EGIDIUS, E.,1987Vibriosis: pathogenicity and pathology-a review. Aquaculture67:15-28.
GALIANA-ARNOUX, D., C. DOSTERT, A. SCHNEEMANN, J. A. HOFFMANN and J. L. IMLER,2006Essential function in vivo for Dicer2in host defense against RNA viruses in Drosophila. Natureimmunology7:590-597.
GANGLOFF, M., A. MURALI, J. XIONG, C. J. ARNOT, A. N. WEBER et al.,2008Structural insight into themechanism of activation of the Toll receptor by the dimeric ligand Sp tzle. Journal of biologicalchemistry283:14629-14635.
GOTO, A., and J. L. IMLER,2012Toll Signaling in klies and mammals: two Sorts of MyD88. Immunity36:555-557.
GOTOH, B., T. KOMATSU, K. TAKEUCHI and J. YOKOO,2002Paramyxovirus the interferon strategies forevading response. Reviews in Medical Virology12:337-357.
HAN-CHING WANG, K., C. W. TSENG, H. Y. LIN, I. T. CHEN, Y. H. CHEN et al.,2010RNAi knock-downof the Litopenaeus vannamei Toll gene (LvToll) significantly increases mortality and reducesbacterial clearance after challenge with Vibrio harveyi. Developmental and comparativeimmunology34:49-58.
HORNG, T., and R. MEDZHITOV,2001Drosophila MyD88is an adapter in the Toll signaling pathway.Proceedings of the National Academy of Sciences of the United States of America98:12654-12658.
HORVATH, C. M.,2004Silencing STATs: lessons from paramyxovirus interferon evasion. Cytokine andgrowth factor reviews15:117-127.
HORVATH, C. M., and J. E. DARNELL,1997The state of the STATs: recent developments in the study ofsignal transduction to the nucleus. Current opinion in cell biology9:233-239.
HOSHINO, K., O. TAKEUCHI, T. KAWAI, H. SANJO, T. OGAWA et al.,1999Cutting edge: Toll-likereceptor4(TLR4)-deficient mice are hyporesponsive to lipopolysaccharide: evidence for TLR4asthe Lps gene product. Journal of immunology162:3749-3752.
HOU, X. S., M. B. MELNICK and N. PERRIMON,1996marelle acts downstream of the DrosophilaHOP/JAK kinase and encodes a protein similar to the mammalian STATs. Cell84:411-419.
HUANG, X. D., Z. X. YIN, X. T. JIA, J. P. LIANG, H. S. AI et al.,2010Identification and functional studyof a shrimp Dorsal homologue. Developmental and comparative immunology34:107-113.
ILIEV, D. B., M. SOBHKHEZ, K. FREMMERLID and J. B. JORGENSEN,2011MyD88Interacts withinterferon regulatory factor (IRF)3and IRF7in Atlantic Salmon (Salmo salar) transgenicSsMyD88modulates the IRF-induced type I interferon response and accumulates in aggresomes.Journal of biological chemistry286:42715-42724.
IMADA, K., and W. J. LEONARD,2000The JAK-STAT pathway. Molecular immunology37:1-11.
IP, Y. T.,2005Drosophila innate immunity goes viral. Nature immunology6:863-863.
JANSSENS, S., and R. BEYAERT,2002A universal role for MyD88in TLR/IL-1R-mediated signaling.Trends in biochemical sciences27:474-482.
JANSSENS, S., K. BURNS, J. TSCHOPP and R. BEYAERT,2002Regulation of interleukin-1-andlipopolysaccharide-induced NF-kappa B activation by alternative splicing of MyD88. Currentbiology12:467-471.
JIRAVANICHPAISAL, P., K. SODERHALL and I. SODERHALL,2006Characterization of white spotsyndrome virus replication in in vitro-cultured haematopoietic stem cells of freshwater crayfish,Pacifastacus leniusculus. Journal of general virology87:847-854.
KAMBRIS, Z., H. BILAK, R. D'ALESSANDRO, M. BELVIN, J. L. IMLER et al.,2003DmMyD88controlsdorsoventral patterning of the Drosophila embryo. EMBO reports4:64-69.
KANEKO, T., and N. SILVERMAN,2005Bacterial recognition and signalling by the Drosophila IMDpathway. Cellular Microbiology7:461-469.
KAWAI, T., O. ADACHI, T. OGAWA, K. TAKEDA and S. AKIRA,1999Unresponsiveness ofMyD88-deficient mice to endotoxin. Immunity11:115-122.
KAWAI, T., and S. AKIRA,2006TLR signaling. Cell death and differentiation13:816-825.
KAWAI, T., and S. AKIRA,2010The role of pattern-recognition receptors in innate immunity: update onToll-like receptors. Nature immunology11:373-384.
KEMP, C., and J. L. IMLER,2009Antiviral immunity in drosophila. Current opinion in immunology21:3-9.
KYBURZ, D., J. RETHAGE, R. SEIBL, R. LAUENER, R. E. GAY et al.,2003Bacterial peptidoglycans butnot CpG oligodeoxynucleotides activate synovial fibroblasts by toll-like receptor signaling.Arthritis and rheumitism48:642-650.
LANDGREN, O., and N. TAGEJA,2014MyD88and beyond: novel opportunities for diagnosis, prognosisand treatment in waldenstr m’s macroglobulinemia. Leukemia27.
LEE, Y. H., and Y. D. YUN,1998HBx protein of hepatitis B virus activates JAK1-STAT signaling.Journal of biological chemistry273:25510-25515.
LEMAITRE, B., and J. HOFFMANN,2007The host defense of Drosophila melanogaster. Annual reviewof immunology25:697-743.
LEMAITRE, B., E. NICOLAS, L. MICHAUT, J. M. REICHHART and J. A. HOFFMANN,1996Thedorsoventral regulatory gene cassette sp tzle/Toll/cactus controls the potent antifungal response inDrosophila adults. Cell86:973-983.
LEONARD, W. J., and J. J. O'SHEA,1998JAKs and STATs: Biological implications. Annual review ofimmunology16:293-322.
LEU, J. H., C. C. CHANG, J. L. WU, C. W. HSU, I. HIRONO et al.,2007Comparative analysis ofdifferentially expressed genes in normal and white spot syndrome virus infected Penaeusmonodon. BMC genomics8.
LEULIER, F., and B. LEMAITRE,2008Toll-like receptors: taking an evolutionary approach. Naturereviews genetics9:165-178.
LEW, D. J., T. DECKER, I. STREHLOW and J. E. DARNELL,1991Overlapping elements in theguanylate-binding protein gene promoter mediate transcriptional induction by alpha and gammainterferons. Molecular and cellular biology11:182-191.
LI, F. H., and J.H. XIANG,2013Signaling pathways regulating innate immune responses in shrimp. Fish&Shellfish Immunology34:973-980.
LI, F. H., D. D. WANG, S. H. LI, H. YAN, J. K. ZHANG et al.,2010A Dorsal homolog (FcDorsal) in theChinese shrimp Fenneropenaeus chinensis is responsive to both bacteria and WSSV challenge.Developmental and comparative immunology34:874-883.
LIN, H. Y., C. H. TANG, Y. H. CHEN, I. H. WEI, J. H. CHEN et al.,2010Peptidoglycan enhancesproinflammatory cytokine expression through the TLR2receptor, MyD88, phosphatidylinositol3-kinase/AKT and NF-kappaB pathways in BV-2microglia. International immunopharmacology10:883-891.
LIN, S. J., H. L. HSIA, W. J. LIU, J. Y. HUANG, K. F. LIU et al.,2012Spawning stress triggers WSSVreplication in brooders via the activation of shrimp STAT. Developmental and comparativeimmunology38:128-135.
LIU, F. S., Y. C. LIU, F. H. LI, B. DONG and J. H. XIANG,2005Molecular cloning and expression profileof putative antilipopolysaccharide factor in Chinese shrimp (Fenneropenaeus chinensis). Marinebiotechnology7:600-608.
LIU, W. J., Y. S. CHANG, A. H. J. WANG, G. H. KOU and C. F. LO,2007White spot syndrome virusannexes a shrimp STAT to enhance expression of the immediate-early gene ie1. Journal ofvirology81:1461-1471.
LIVAK, K. J., and T. D. SCHMITTGEN,2001Analysis of relative gene expression data using real-timequantitative PCR and the2-ΔΔCtmethod. Methods25:402-408.
LORD, K. A., B. HOFFMANLIEBERMANN and D. A. LIEBERMANN,1990Nucleotide sequence andexpression of a cDNA encoding Myd88, a novel myeloid differentiation primary response geneinduced by IL6. Oncogene5:1095-1097.
LUO, H., and C. R. DEAROLF,2001The JAK/STAT pathway and Drosophila development. Bioessays23:1138-1147.
LUO, T., H. J. YANG, F. LI, X. B. ZHANG and X. XU,2006Purification, characterization and cDNAcloning of a novel lipopolysaccharide-binding lectin from the shrimp Penaeus monodon.Developmental and comparative immunology30:607-617.
LUO, Z., J. Q. ZHANG, F. H. LI, X. J. ZHANG, C. Z. LIU et al.,2011Identification of a novel C-typelectin from the shrimp Litopenaeus vannamei and its role in defense against pathogens infection.Chinese journal of oceanology and limnology29:942-951.
MAI, W. J., and W. N. WANG,2010Protection of blue shrimp (Litopenaeus stylirostris) against theWhite Spot Syndrome Virus (WSSV) when injected with shrimp lysozyme. Fish and shellfishimmunology28:727-733.
MAREK, L. R., and J. C. KAGAN,2012Phosphoinositide binding by the Toll adaptor dMyD88controlsantibacterial responses in Drosophila. Immunity36:612-622.
MATSUI, K., M. WIROTESANGTHONG and A. NISHIKAWA,2009Analysis of signaling pathways involvedin peptidoglycan-induced RANTES production from murine langerhans cells. Internationalarchives of allergy and immunology149:167-172.
MCGETTRICK, A. F., and L. A. J. O'NEILL,2004The expanding family of MyD88-like adaptors inToll-like receptor signal transduction. Molecular immunology41:577-582.
MEDZHITOV, R., P. PRESTONHURLBURT and C. A. JANEWAY,1997A human homologue of theDrosophila Toll protein signals activation of adaptive immunity. Nature388:394-397.
MEIJER, A. H., S. F. G. KRENS, I. A. M. RODRIGUEZ, S. N. HE, W. BITTER et al.,2004Expressionanalysis of the Toll-like receptor and TIR domain adaptor families of zebrafish. Molecularimmunology40:773-783.
MEKATA, T., T. KONO, T. YOSHIDA, M. SAKAI and T. ITAMI,2008Identification of cDNA encoding Tollreceptor, MjToll gene from kuruma shrimp, Marsupenaeus japonicus. Fish and shellfishimmunology24:122-133.
MEKATA, T., R. SUDHAKARAN, S. OKUGAWA, M. INADA, T. KONO et al.,2010A novel gene of tumornecrosis factor ligand superfamily from kuruma shrimp, Marsupenaeus japonicus. Fish&Shellfish Immunology28:571-578.
MORTAZAVI, A., B. A. WILLIAMS, K. MCCUE, L. SCHAEFFER and B. WOLD,2008Mapping andquantifying mammalian transcriptomes by RNA-seq. Nature methods5:621-628.
MURRAY, P. J.,2007The JAK/STAT signaling pathway: input and output intergration. Journal ofimmunology178:2623-2629.
O'BRIEN, V.,1998Viruses and apoptosis. Journal of general virology79:1833-1845.
OIE,2009World organisation for animal health. Aquatic Animal Health Code.
PALTI, Y.,2011Toll-like receptors in bony fish: from genomics to function. Developmental andcomparative immunology35:1263-1272.
PARADKAR, P. N., L. TRINIDAD, R. VOYSEY, J. B. DUCHEMIN and P. J. WALKER,2012Secreted vagorestricts West Nile virus infection in Culex mosquito cells by activating the JAK-STAT pathway.Proceedings of the National Academy of Sciences of the United States of America109:18915-18920.
PARK, E. H., S. S. KOH, R. SRISUTTEE, I. R. CHO, H. J. MIN et al.,2009Expression of HBX, anoncoprotein of Hepatitis B virus, blocks reoviral oncolysis of hepatocellular carcinoma cells.Cancer gene therapy16:453-461.
PONGSOMBOON, S., S. TANG, S. BOONDA, T. AOKI, I. HIRONO et al.,2011A cDNA microarray approachfor analyzing transcriptional changes in Penaeus monodon after infection by pathogens. Fish andshellfish immunology30:439-446.
QIAO, J., Z. H. DU, Y. L. ZHANG, H. DU, L. L. GUO et al.,2011Proteomic identification of the relatedimmune enhancing proteins in shrimp Litopenaeus vannamei stimulated with vitamin C andChinese herbs. Fish and shellfish immunology31:736-745.
QIU, L. M., L. S. SONG, Y. D. YU, W. XU, D. J. NI et al.,2007Identification and characterization of amyeloid differentiation factor88(MyD88) cDNA from Zhikong scallop Chlamys farreri. Fish andshellfish immunology23:614-623.
RAN, X. Z., X. F. BIAN, Y. C. JI, X. M. YAN, F. YANG et al.,2013White Spot Syndrome Virus IE1andWSV056modulate the G(1)/S Transition by binding to the host retinoblastoma protein. Journal ofvirology87:12576-12582.
RATTANACHAI, A., I. HIRONO, T. OHIRA, Y. TAKAHASHI and T. AOKI,2004Cloning of kuruma prawnMarsupenaeus japonicus crustin-like peptide cDNA and analysis of its expressiony. Fisheriesscience70:765-771.
REBL, A., T. GOLDAMMER, U. FISCHER, B. KOLLNER and H. M. SEYFERT,2009Characterization of twokey molecules of teleost innate immunity from rainbow trout (Oncorhynchus mykiss): MyD88andSAA. Veterinary immunology and immunopathology131:122-126.
ROBALINO, J., C. L. BROWDY, S. PRIOR, A. METZ, P. PARNELL et al.,2004Induction of antiviralimmunity by double stranded RNA in a marine invertebrate. Journal of virology78:10442-10448.
ROWLEY, A. F., and A. POWELL,2007Invertebrate immune systems specific, quasi specific, ornonspecific? Journal of immunology179:7209-7214.
RUSCANU, S., F. PASCALE, M. BOURGE, B. HEMATI, J. ELHMOUZI-YOUNES et al.,2012The Doublestranded RNA bluetongue virus induces type I interferon in plasmacytoid dendritic cells via aMyD88-dependent TLR7/8-independent signaling pathway. Journal of Vvirology86:5817-5828.
RUTSCHMANN, S., A. KILINC and D. FERRANDON,2002Cutting edge: The Toll pathway is required forresistance to Gram-positive bacterial infections in Drosophila. Journal of immunology168:1542-1546.
S DERH LL, I., E. BANGYEEKHUN, S. MAYO and K. S DERH LL,2003Hemocyte production andmaturation in an invertebrate animal; proliferation and gene expression in hematopoietic stemcells of Pacifastacus leniusculus. Developmental and comparative immunology27:661-672.
SABIN, L. R., S. L. HANNA and S. CHERRY,2010Innate antiviral immunity in Drosophila. Currentopinion in immunology22:4-9.
SALCEDO, R., C. CATAISSON, U. HASAN, S. H. YUSPA and G. TRINCHIERI,2013MyD88and itsdivergent toll in carcinogenesis. Trends in immunology34:379-389.
SAMUEL, C. E.,2001Antiviral actions of interferons. Clinical microbiology reviews14:778-809.
SEGOVIA, J., A. SABBAH, V. MGBEMENA, S.-Y. TSAI, T.-H. CHANG et al.,2012TLR2/MyD88/NF-kappa B pathway, reactive oxygen species, potassium efflux activatesNLRP3/ASC inflammasome during respiratory syncytial virus infection. Plos one7.
SHI, X. Z., R. R. ZHANG, Y. P. JIA, X. F. ZHAO, X. Q. YU et al.,2009Identification and molecularcharacterization of a Sp tzle-like protein from Chinese shrimp (Fenneropenaeus chinensis). Fishand shellfish immunology27:610-617.
SHI, Z., C. HUANG, J. ZHANG, D. CHEN and J. BONAMI,2000White spot syndrome virus (WSSV)experimental infection of the freshwater crayfish, Cherax quadricarinatus. Journal of fish diseases23:285-288.
SKJAEVELAND, I., D. B. ILIEV, G. STRANDSKOG and J. B. JORGENSEN,2009Identification andcharacterization of TLR8and MyD88homologs in Atlantic salmon (Salmo salar). Developmentaland comparative immunology33:1011-1017.
SOLDAINI, E., S. JOHN, S. MORO, J. BOLLENBACHER, U. SCHINDLER et al.,2000DNA binding siteselection of dimeric and tetrameric Stat5proteins reveals a large repertoire of divergent tetramericStat5a binding sites. Molecular and cellular biology20:389-401.
STARK, G. R., and J. E. DARNELL,2012The JAK/STAT pathway at twenty. Immunity36:503-514.
STENTIFORD, G. D., J. R. BONAMI and V. ALDAY-SANZ,2009A critical review of susceptibility ofcrustaceans to taura syndrome, yellow head disease and white spot disease and implications ofinclusion of these diseases in European legislation. Aquaculture291:1-17.
SUN, C., H. L. SHAO, X. W. ZHANG, X. F. ZHAO and J. X. WANG,2011Molecular cloning andexpression analysis of signal transducer and activator of transcription (STAT) from the Chinesewhite shrimp Fenneropenaeus chinensis. Molecular biology reports38:5313-5319.
SUN, Y. M., F. H. LI and J. H. XIANG,2013a Analysis on the dynamic changes of the amount of WSSVin Chinese shrimp Fenneropenaeus chinensis during infection. Aquaculture376–379:124-132.
SUN, Y. M., F. H. LI, Z. SUN, X. J. ZHANG, S. H. LI et al.,2014Transcriptome analysis of the initialstage of acute WSSV infection caused by temperature change. Plos one9.
SUN, Y. M., F. H. LI and J. H. XIANG,2013b Analysis on the dynamic changes of the amount of WSSVin Chinese shrimp Fenneropenaeus chinensis during infection. Aquaculture376:124-132.
TAHIRI-ALAOUI, A., L. P. SMITH, S. BAIGENT, L. KGOSANA, L. J. PETHERBRIDGE et al.,2009Identification of an intercistronic internal ribosome entry site in a Marek's disease virus immediateearly gene. Journal of virology83:5846-5853.
TAKANO, T., H. KONDO, I. HIRONO, T. SAITO-TAKI, M. ENDO et al.,2006Identification andcharacterization of a myeloid differentiation factor88(MyD88) cDNA and gene in Japaneseflounder, Paralichthys olivaceus. Developmental and comparative immunology30:807-816.
TAKAOKA, A., and H. YANAI,2006Interferon signalling network in innate defence. Cellularmicrobiology8:907-922.
TALREJA, J., M. H. KABIR, M. B. FILLA, D. J. STECHSCHULTE and K. N. DILEEPAN,2004Histamineinduces Toll-like receptor2and4expression in endothelial cells and enhances sensitivity toGram-positive and Gram-negative bacterial cell wall components. Immunology113:224-233.
TASSANAKAJON, A., K. SOMBOONWIWAT, P. SUPUNGUL and S. TANG,2013Discovery of immunemolecules and their crucial functions in shrimp immunity. Fish and shellfish immunology34:954-967.
TAUSZIG-DELAMASURE, S., H. BILAK, M. CAPOVILLA, J. A. HOFFMANN and J. L. IMLER,2002Drosophila MyD88is required for the response to fungal and Gram-positive bacterial infections.Nature immunology3:91-97.
THARNTADA, S., S. PONPRATEEP, K. SOMBOONWIWAT, H. P. LIU, I. SODERHALL et al.,2009Role ofanti-lipopolysaccharide factor from the black tiger shrimp, Penaeus monodon, in protection fromwhite spot syndrome virus infection. Journal of general virology90:1491-1498.
TYAGI, A., R. KHUSHIRAMANI, I. KARUNASAGAR and I. KARUNASAGAR,2007Antivibrio activity ofrecombinant lysozyme expressed from black tiger shrimp, Penaeus monodon. Aquaculture272:246-253.
VALANNE, S., J. H. WANG and M. RAMET,2011The Drosophila Toll signaling pathway. Journal ofimmunology186:649-656.
VAN DER SAR, A. M., O. W. STOCKHAMMER, C. VAN DER LAAN, H. P. SPAINK, W. BITTER et al.,2006aMyD88innate immune function in a zebrafish embryo infection model. Infection and immunity74:2436-2441.
VAN DER SAR, A. M., O. W. STOCKHAMMER, C. VAN DER LAAN, H. P. SPAINK, W. BITTER et al.,2006bMyD88innate immune function in a zebrafish embryo infection model. Infection and immunity74:2436.
VAN RIJ, R. P., M. C. SALEH, B. BERRY, C. FOO, A. HOUK et al.,2006The RNA silencing endonucleaseArgonaute2mediates specific antiviral immunity in Drosophila melanogaster. Genes anddevelopment20:2985-2995.
WANG, D., F. LI, S. LI, Y. CHI, R. WEN et al.,2012a An IkappaB homologue (FcCactus) in Chineseshrimp Fenneropenaeus chinensis. Developmental and comparative immunology39:352-362.
WANG, L. Y., F. H. LI, B. WANG and J. H. XIANG,2013A new shrimp peritrophin-like gene fromExopalaemon carinicauda involved in white spot syndrome virus (WSSV) infection. Fish andshellfish immunology35:840-846.
WANG, P., J. LIANG, Z. GU, D. WAN, S. WENG et al.,2012b Molecular cloning, characterization andexpression analysis of two novel Tolls (LvToll2and LvToll3) and three putative Sp tzle-like Tollligands (LvSpz1–3) from Litopenaeus vannamei. Developmental and comparative immunology36:359-371.
WANG, P., D. WAN, Z. GU, X. DENG, S. WENG et al.,2011A Litopenaeus vannamei tumor necrosisfactor receptor-associated factor6(TRAF6) responds to Vibrio alginolyticus and white spotsyndrome virus (WSSV) infection and activates antimicrobial peptide genes. Developmental andcomparative immunology35:105-114.
WANG, P. H., Z. H. GU, D. H. WAN, M. Y. ZHANG, S. P. WENG et al.,2011b The shrimp NF-kappa Bpathway is activated by White Spot Syndrome Virus (WSSV)449to facilitate the expression ofwsv069(ie1), wsv303and wsv371. Plos one6.
WANG, P. H., D. H. WAN, L. R. PANG, Z. H. GU, W. QIU et al.,2012c Molecular cloning,characterization and expression analysis of the tumor necrosis factor (TNF) superfamily gene,TNF receptor superfamily gene and lipopolysaccharide-induced TNF-alpha factor (LITAF) genefrom Litopenaeus vannamei. Developmental and comparative immunology36:39-50.
WARIS, G., K. W. HUH and A. SIDDIQUI,2001Mitochondrially associated hepatitis B virus X proteinconstitutively activates transcription factors STAT3and NF-kappa B via oxidative stress.Molecular and cellular biology21:7721-7730.
WARIS, G., J. TURKSON, T. HASSANEIN and A. SIDDIQUI,2005Hepatitis C virus (HCV) constitutivelyactivates STAT3via oxidative stress: role of STAT3in HCV replication. Journal of virology79:1569-1580.
WESCHE, H., W. J. HENZEL, W. SHILLINGLAW, S. LI and Z. D. CAO,1997MyD88: an adapter thatrecruits IRAK to the IL-1receptor complex. Immunity7:837-847.
WONGPRASERT, K., K. KHANOBDEE, S. S. GLUNUKARN, P. MEERATANA and B. WITHYACHUMNARNKUL,2003Time course and levels of apoptosis in various tissues of black tiger shrimp Penaeusmonodon infected with White Spot Syndrome Virus. Diseases of aquatic organisms55:3-10.
XI, Z. Y., J. L. RAMIREZ and G. DIMOPOULOS,2008The Aedes aegypti Toll pathway controls denguevirus infection. Plos pathogens4.
XU, X. A., Y. L. SUN and T. HOEY,1996Cooperative DNA binding and sequence selective recognitionconferred by the STAT amino terminal domain. Science273:794-797.
YAFEI DUAN, P. L., JITAO LI, JIAN LI1, YUN WANG AND PING CHEN,2014The responsive expression ofa chitinase gene in the ridgetail white prawn Exopalaemon carinicauda against Vibrioanguillarum and WSSV challenge. Cell Stress and Chaperones.
YAN, Y., H. C. CUI, J. G. WEI, Y. H. HUANG, X. H. HUANG et al.,2012Functional genomic studies onan immune-and antiviral-related gene of MyD88in orange-spotted grouper, Epinephelus coioides.Chinese science bulletin57:3277-3287.
YANG, C. J., J. Q. ZHANG, F. H. LI, H. M. MA, Q. L. ZHANG et al.,2008A toll receptor from Chineseshrimp Fenneropenaeus chinensis is responsive to Vibrio anguillarum infection. Fish and shellfishimmunology24:564-574.
YANG, L. S., Z. X. YIN, J. X. LIAO, X. D. HUANG, C. J. GUO et al.,2007A Toll receptor in shrimp.Molecular immunology44:1999-2008.
YAO, C. L., P. KONG, Z. Y. WANG, P. F. JI, X. D. LIU et al.,2009Molecular cloning and expression ofMyD88in large yellow croaker, Pseudosciaena crocea. Fish and shellfish immunology26:249-255.
YU, F. F., Y. B. ZHANG, T. K. LIU, Y. LIU, F. SUN et al.,2010Fish virus-induced interferon exertsantiviral function through Stat1pathway. Molecular immunology47:2330-2341.
YU, X. Q., H. GAN and M. R. KANOST,1999Immulectin, an inducible C-type lectin from an insect,Manduca sexta, stimulates activation of plasma prophenol oxidase. Insect biochemistry andmolecular biology29:585-597.
ZAMBON, R. A., M. NANDAKUMAR, V. N. VAKHARIA and L. P. WU,2005The Toll pathway is importantfor an antiviral response in Drosophila. Proceedings of the National Academy of Sciences of theUnited States of America102:7257-7262.
ZAMBON, R. A., V. N. VAKHARIA and L. P. WU,2006RNAi is an antiviral immune response against adsRNA virus in Drosophila melanogaster. Cellular microbiology8:880-889.
ZEIDLER, M. P., E. A. BACH and N. PERRIMON,2000The roles of the Drosophila JAK/STAT pathway.Oncogene19:2598-2606.
ZHAN, W. B., Y. H. WANG, J. L. FRYER, K. OKUBO, H. FUKUDA et al.,1999Production of monoclonalantibodies (MAbs) against White Spot Syndrome Virus (WSSV). Journal of aquatic animal health11:17-22.
ZHANG, S., C. Z. LI, H. YAN, W. QIU, Y. G. CHEN et al.,2012Identification and function of myeloiddifferentiation factor88(MyD88) in Litopenaeus vannamei. Plos one7.
ZHANG, Y., and J. GUI,2004Molecular characterization and IFN signal pathway analysis of Carassiusauratus CaSTAT1identified from the cultured cells in response to virus infection. Developmentaland comparative immunology28:211-227.
ZHAO, Z. Y., Z. X. YIN, S. P. WENG, H. J. GUAN, S. D. LI et al.,2007Profiling of differentiallyexpressed genes in hepatopancreas of White Spot Syndrome Virus-resistant shrimp (Litopenaeusvannamei) by suppression subtractive hybridisation. Fish and shellfish immunology22:520-534.
朱艳冰和杨丰,2005对虾白斑综合症病毒类锌指蛋白基因(wsv063、wsv069和wsv477)的表达和纯化.厦门大学学报(自然科学版):697-700.
李钫,2009对虾白斑综合症病毒极早期基因的研究,厦门大学.
易志刚,黄倢和李筠,2004WSSV结构、功能及基因组学研究概况.海洋水产研究:79-84.
相建海,2001海水养殖生物病害发生与控制.海洋出版社.
高溳,金春英和王蔚,2011对虾白斑综合症病毒IE1表达与鉴定.中国公共卫生:535-536.
吴月喜,2011对虾弧菌病诱因与防控措施.海洋与渔业10:54.
张志栋,战文斌,薛艳红和邢婧,2005凡纳滨对虾(Litopenaeus vannamei)感染白斑症病毒(WSSV)后大颗粒血细胞的变化.海洋与湖沼:67-71.
AKIRA, S., K. TAKEDA and T. KAISHO,2001Toll-like receptors: critical proteins linking innate andacquired immunity. Nature immunology2:675-680.
AYYAZ, A., P. GIAMMARINARO, S. LIEGEOIS, M. LESTRADET and D. FERRANDON,2013A negative rolefor MyD88in the resistance to starvation as revealed in an intestinal infection of Drosophilamelanogaster with the Gram-positive bacterium Staphylococcus xylosus. Immunobiology218:635-644.
BARTON, G. M., and J. C. KAGAN,2009A cell biological view of Toll-like receptor function: regulationthrough compartmentalization. Nature reviews immunology9:535-542.
BASU, M., B. SWAIN, B. R. SAHOO, N. K. MAITI and M. SAMANTA,2012Induction of toll-like receptor(TLR)2, and MyD88-dependent TLR-signaling in response to ligand stimulation and bacterialinfections in the Indian major carp, mrigal (Cirrhinus mrigala). Molecular biology reports39:6015-6028.
BENEDICT, C. A., P. S. NORRIS and C. F. WARE,2002To kill or be killed: viral evasion of apoptosis.Nature immunology3:1013-1018.
BONILLA, C. M. E.,2011Application of RNA interference (RNAi) against viral infections in shrimp: areview. Journal of antivirals and antiretrovirals05.
BONNERT, T. P., K. E. GARKA, P. PARNET, G. SONODA, J. R. TESTA et al.,1997The cloning andcharacterization of human MyD88: A member of an IL-1receptor related family. FEBS letters402:81-84.
BURGE, E. J., D. J. MADIGAN, L. E. BURNETT and K. G. BURNETT,2007Lysozyme gene expression byhemocytes of Pacific white shrimp, Litopenaeus vannamei, after injection with Vibrio. Fish andshellfish immunology22:327-339.
CAIPANG, C. M. A., I. HIRONO and T. AOKI,2005Induction of antiviral state in fish cells by Japaneseflounder, Paralichthys olivaceus, interferon regulatory factor-1. Fish and shellfish immunology19:79-91.
CASANOVA, J. L., L. ABEL and L. QUINTANA-MURCI,2011Human TLRs and IL-1Rs in host defense:natural insights from evolutionary, epidemiological, and clinical genetics. Annual review ofimmunology29:447-491.
CHANG, P. S., C. F. LO, Y. C. WANG and G. H. KOU,1996Identification of white spot syndromeassociated baculovirus (WSBV) target organs in the shrimp Penaeus monodon by in situhybridization. Diseases of aquatic organisms27:131-139.
CHEN, I. T., T. AOKI, Y. T. HUANG, I. HIRONO, T. C. CHEN et al.,2011White Spot Syndrome Virusinduces metabolic changes resembling the Warburg effect in shrimp hemocytes in the early stageof infection. Journal of virology85:12919-12928.
CHEN, W. Y., K. C. HO, J. H. LEU, K. F. LIU, H. C. WANG et al.,2008WSSV infection activates STATin shrimp. Developmental and comparative immunology32:1142-1150.
COPELAND, N. G., D. J. GILBERT, C. SCHINDLER, Z. ZHONG, Z. WEN et al.,1995Distribution of themammalian stat gene family in mouse chromosomes. Genomics29:225-228.
CRONIN, J. G., M. L. TURNER, L. GOETZE, C. E. BRYANT and I. M. SHELDON,2011Toll-like receptor4and MyD88-dependent signaling mechanisms of the innate immune system are essential for theresponse to Lipopolysaccharide by epithelial and stromal cells of the bovine endometrium.Biology of reproduction86(2):1-9.
DARNELL, J. E.,1997STATs and gene regulation. Science277:1630-1635.
DAVID, S. A., and V. I. MATHAN,1993Enzyme-linked immunosorbent assay for quantification ofanti-lipid a antibody: enhancement of sensitivity by antigen immobilization at low pH. Journal ofimmunological methods163:85-89.
DE LA PE A, L. D., T. TAMAKI, K. MOMOYAMA, T. NAKAI and K. MUROGA,1993Characteristics of thecausative bacterium of vibriosis in the kuruma prawn, Penaeus japonicus. Aquaculture115:1-12.
DE LA VEGA, E., N. A. O'LEARY, J. E. SHOCKEY, J. ROBALINO, C. PAYNE et al.,2008Anti-lipopolysaccharide factor in Litopenaeus vannamei (LvALF): a broad spectrum antimicrobialpeptide essential for shrimp immunity against bacterial and fungal infection. Molecularimmunology45:1916-1925.
DE ZOYSA, M., S. JUNG and J. LEE,2009First molluscan TNF-alpha homologue of the TNFsuperfamily in disk abalone: molecular characterization and expression analysis. Fish&shellfishimmunology26:625-631.
DING, S. W., and O. VOINNET,2007Antiviral immunity directed by small RNAs. Cell130:413-426.
DOSTERT, C., E. JOUANGUY, P. IRVING, L. TROXLER, D. GALIANA-ARNOUX et al.,2005The JAK-STATsignaling pathway is required but not sufficient for the antiviral response of Drosophila. Natureimmunology6:946-953.
EDITORS, T. D. M. M.,2012Jak-Stat Signaling: from basics to disease. Springer.
EGIDIUS, E.,1987Vibriosis: pathogenicity and pathology-a review. Aquaculture67:15-28.
GALIANA-ARNOUX, D., C. DOSTERT, A. SCHNEEMANN, J. A. HOFFMANN and J. L. IMLER,2006Essential function in vivo for Dicer2in host defense against RNA viruses in Drosophila. Natureimmunology7:590-597.
GANGLOFF, M., A. MURALI, J. XIONG, C. J. ARNOT, A. N. WEBER et al.,2008Structural insight into themechanism of activation of the Toll receptor by the dimeric ligand Sp tzle. Journal of biologicalchemistry283:14629-14635.
GOTO, A., and J. L. IMLER,2012Toll Signaling in klies and mammals: two Sorts of MyD88. Immunity36:555-557.
GOTOH, B., T. KOMATSU, K. TAKEUCHI and J. YOKOO,2002Paramyxovirus the interferon strategies forevading response. Reviews in Medical Virology12:337-357.
HAN-CHING WANG, K., C. W. TSENG, H. Y. LIN, I. T. CHEN, Y. H. CHEN et al.,2010RNAi knock-downof the Litopenaeus vannamei Toll gene (LvToll) significantly increases mortality and reducesbacterial clearance after challenge with Vibrio harveyi. Developmental and comparativeimmunology34:49-58.
HORNG, T., and R. MEDZHITOV,2001Drosophila MyD88is an adapter in the Toll signaling pathway.Proceedings of the National Academy of Sciences of the United States of America98:12654-12658.
HORVATH, C. M.,2004Silencing STATs: lessons from paramyxovirus interferon evasion. Cytokine andgrowth factor reviews15:117-127.
HORVATH, C. M., and J. E. DARNELL,1997The state of the STATs: recent developments in the study ofsignal transduction to the nucleus. Current opinion in cell biology9:233-239.
HOSHINO, K., O. TAKEUCHI, T. KAWAI, H. SANJO, T. OGAWA et al.,1999Cutting edge: Toll-likereceptor4(TLR4)-deficient mice are hyporesponsive to lipopolysaccharide: evidence for TLR4asthe Lps gene product. Journal of immunology162:3749-3752.
HOU, X. S., M. B. MELNICK and N. PERRIMON,1996marelle acts downstream of the DrosophilaHOP/JAK kinase and encodes a protein similar to the mammalian STATs. Cell84:411-419.
HUANG, X. D., Z. X. YIN, X. T. JIA, J. P. LIANG, H. S. AI et al.,2010Identification and functional studyof a shrimp Dorsal homologue. Developmental and comparative immunology34:107-113.
ILIEV, D. B., M. SOBHKHEZ, K. FREMMERLID and J. B. JORGENSEN,2011MyD88Interacts withinterferon regulatory factor (IRF)3and IRF7in Atlantic Salmon (Salmo salar) transgenicSsMyD88modulates the IRF-induced type I interferon response and accumulates in aggresomes.Journal of biological chemistry286:42715-42724.
IMADA, K., and W. J. LEONARD,2000The JAK-STAT pathway. Molecular immunology37:1-11.
IP, Y. T.,2005Drosophila innate immunity goes viral. Nature immunology6:863-863.
JANSSENS, S., and R. BEYAERT,2002A universal role for MyD88in TLR/IL-1R-mediated signaling.Trends in biochemical sciences27:474-482.
JANSSENS, S., K. BURNS, J. TSCHOPP and R. BEYAERT,2002Regulation of interleukin-1-andlipopolysaccharide-induced NF-kappa B activation by alternative splicing of MyD88. Currentbiology12:467-471.
JIRAVANICHPAISAL, P., K. SODERHALL and I. SODERHALL,2006Characterization of white spotsyndrome virus replication in in vitro-cultured haematopoietic stem cells of freshwater crayfish,Pacifastacus leniusculus. Journal of general virology87:847-854.
KAMBRIS, Z., H. BILAK, R. D'ALESSANDRO, M. BELVIN, J. L. IMLER et al.,2003DmMyD88controlsdorsoventral patterning of the Drosophila embryo. EMBO reports4:64-69.
KANEKO, T., and N. SILVERMAN,2005Bacterial recognition and signalling by the Drosophila IMDpathway. Cellular Microbiology7:461-469.
KAWAI, T., O. ADACHI, T. OGAWA, K. TAKEDA and S. AKIRA,1999Unresponsiveness ofMyD88-deficient mice to endotoxin. Immunity11:115-122.
KAWAI, T., and S. AKIRA,2006TLR signaling. Cell death and differentiation13:816-825.
KAWAI, T., and S. AKIRA,2010The role of pattern-recognition receptors in innate immunity: update onToll-like receptors. Nature immunology11:373-384.
KEMP, C., and J. L. IMLER,2009Antiviral immunity in drosophila. Current opinion in immunology21:3-9.
KYBURZ, D., J. RETHAGE, R. SEIBL, R. LAUENER, R. E. GAY et al.,2003Bacterial peptidoglycans butnot CpG oligodeoxynucleotides activate synovial fibroblasts by toll-like receptor signaling.Arthritis and rheumitism48:642-650.
LANDGREN, O., and N. TAGEJA,2014MyD88and beyond: novel opportunities for diagnosis, prognosisand treatment in waldenstr m’s macroglobulinemia. Leukemia27.
LEE, Y. H., and Y. D. YUN,1998HBx protein of hepatitis B virus activates JAK1-STAT signaling.Journal of biological chemistry273:25510-25515.
LEMAITRE, B., and J. HOFFMANN,2007The host defense of Drosophila melanogaster. Annual reviewof immunology25:697-743.
LEMAITRE, B., E. NICOLAS, L. MICHAUT, J. M. REICHHART and J. A. HOFFMANN,1996Thedorsoventral regulatory gene cassette sp tzle/Toll/cactus controls the potent antifungal response inDrosophila adults. Cell86:973-983.
LEONARD, W. J., and J. J. O'SHEA,1998JAKs and STATs: Biological implications. Annual review ofimmunology16:293-322.
LEU, J. H., C. C. CHANG, J. L. WU, C. W. HSU, I. HIRONO et al.,2007Comparative analysis ofdifferentially expressed genes in normal and white spot syndrome virus infected Penaeusmonodon. BMC genomics8.
LEULIER, F., and B. LEMAITRE,2008Toll-like receptors: taking an evolutionary approach. Naturereviews genetics9:165-178.
LEW, D. J., T. DECKER, I. STREHLOW and J. E. DARNELL,1991Overlapping elements in theguanylate-binding protein gene promoter mediate transcriptional induction by alpha and gammainterferons. Molecular and cellular biology11:182-191.
LI, F. H., and J.H. XIANG,2013Signaling pathways regulating innate immune responses in shrimp. Fish&Shellfish Immunology34:973-980.
LI, F. H., D. D. WANG, S. H. LI, H. YAN, J. K. ZHANG et al.,2010A Dorsal homolog (FcDorsal) in theChinese shrimp Fenneropenaeus chinensis is responsive to both bacteria and WSSV challenge.Developmental and comparative immunology34:874-883.
LIN, H. Y., C. H. TANG, Y. H. CHEN, I. H. WEI, J. H. CHEN et al.,2010Peptidoglycan enhancesproinflammatory cytokine expression through the TLR2receptor, MyD88, phosphatidylinositol3-kinase/AKT and NF-kappaB pathways in BV-2microglia. International immunopharmacology10:883-891.
LIN, S. J., H. L. HSIA, W. J. LIU, J. Y. HUANG, K. F. LIU et al.,2012Spawning stress triggers WSSVreplication in brooders via the activation of shrimp STAT. Developmental and comparativeimmunology38:128-135.
LIU, F. S., Y. C. LIU, F. H. LI, B. DONG and J. H. XIANG,2005Molecular cloning and expression profileof putative antilipopolysaccharide factor in Chinese shrimp (Fenneropenaeus chinensis). Marinebiotechnology7:600-608.
LIU, W. J., Y. S. CHANG, A. H. J. WANG, G. H. KOU and C. F. LO,2007White spot syndrome virusannexes a shrimp STAT to enhance expression of the immediate-early gene ie1. Journal ofvirology81:1461-1471.
LIVAK, K. J., and T. D. SCHMITTGEN,2001Analysis of relative gene expression data using real-timequantitative PCR and the2-ΔΔCtmethod. Methods25:402-408.
LORD, K. A., B. HOFFMANLIEBERMANN and D. A. LIEBERMANN,1990Nucleotide sequence andexpression of a cDNA encoding Myd88, a novel myeloid differentiation primary response geneinduced by IL6. Oncogene5:1095-1097.
LUO, H., and C. R. DEAROLF,2001The JAK/STAT pathway and Drosophila development. Bioessays23:1138-1147.
LUO, T., H. J. YANG, F. LI, X. B. ZHANG and X. XU,2006Purification, characterization and cDNAcloning of a novel lipopolysaccharide-binding lectin from the shrimp Penaeus monodon.Developmental and comparative immunology30:607-617.
LUO, Z., J. Q. ZHANG, F. H. LI, X. J. ZHANG, C. Z. LIU et al.,2011Identification of a novel C-typelectin from the shrimp Litopenaeus vannamei and its role in defense against pathogens infection.Chinese journal of oceanology and limnology29:942-951.
MAI, W. J., and W. N. WANG,2010Protection of blue shrimp (Litopenaeus stylirostris) against theWhite Spot Syndrome Virus (WSSV) when injected with shrimp lysozyme. Fish and shellfishimmunology28:727-733.
MAREK, L. R., and J. C. KAGAN,2012Phosphoinositide binding by the Toll adaptor dMyD88controlsantibacterial responses in Drosophila. Immunity36:612-622.
MATSUI, K., M. WIROTESANGTHONG and A. NISHIKAWA,2009Analysis of signaling pathways involvedin peptidoglycan-induced RANTES production from murine langerhans cells. Internationalarchives of allergy and immunology149:167-172.
MCGETTRICK, A. F., and L. A. J. O'NEILL,2004The expanding family of MyD88-like adaptors inToll-like receptor signal transduction. Molecular immunology41:577-582.
MEDZHITOV, R., P. PRESTONHURLBURT and C. A. JANEWAY,1997A human homologue of theDrosophila Toll protein signals activation of adaptive immunity. Nature388:394-397.
MEIJER, A. H., S. F. G. KRENS, I. A. M. RODRIGUEZ, S. N. HE, W. BITTER et al.,2004Expressionanalysis of the Toll-like receptor and TIR domain adaptor families of zebrafish. Molecularimmunology40:773-783.
MEKATA, T., T. KONO, T. YOSHIDA, M. SAKAI and T. ITAMI,2008Identification of cDNA encoding Tollreceptor, MjToll gene from kuruma shrimp, Marsupenaeus japonicus. Fish and shellfishimmunology24:122-133.
MEKATA, T., R. SUDHAKARAN, S. OKUGAWA, M. INADA, T. KONO et al.,2010A novel gene of tumornecrosis factor ligand superfamily from kuruma shrimp, Marsupenaeus japonicus. Fish&Shellfish Immunology28:571-578.
MORTAZAVI, A., B. A. WILLIAMS, K. MCCUE, L. SCHAEFFER and B. WOLD,2008Mapping andquantifying mammalian transcriptomes by RNA-seq. Nature methods5:621-628.
MURRAY, P. J.,2007The JAK/STAT signaling pathway: input and output intergration. Journal ofimmunology178:2623-2629.
O'BRIEN, V.,1998Viruses and apoptosis. Journal of general virology79:1833-1845.
OIE,2009World organisation for animal health. Aquatic Animal Health Code.
PALTI, Y.,2011Toll-like receptors in bony fish: from genomics to function. Developmental andcomparative immunology35:1263-1272.
PARADKAR, P. N., L. TRINIDAD, R. VOYSEY, J. B. DUCHEMIN and P. J. WALKER,2012Secreted vagorestricts West Nile virus infection in Culex mosquito cells by activating the JAK-STAT pathway.Proceedings of the National Academy of Sciences of the United States of America109:18915-18920.
PARK, E. H., S. S. KOH, R. SRISUTTEE, I. R. CHO, H. J. MIN et al.,2009Expression of HBX, anoncoprotein of Hepatitis B virus, blocks reoviral oncolysis of hepatocellular carcinoma cells.Cancer gene therapy16:453-461.
PONGSOMBOON, S., S. TANG, S. BOONDA, T. AOKI, I. HIRONO et al.,2011A cDNA microarray approachfor analyzing transcriptional changes in Penaeus monodon after infection by pathogens. Fish andshellfish immunology30:439-446.
QIAO, J., Z. H. DU, Y. L. ZHANG, H. DU, L. L. GUO et al.,2011Proteomic identification of the relatedimmune enhancing proteins in shrimp Litopenaeus vannamei stimulated with vitamin C andChinese herbs. Fish and shellfish immunology31:736-745.
QIU, L. M., L. S. SONG, Y. D. YU, W. XU, D. J. NI et al.,2007Identification and characterization of amyeloid differentiation factor88(MyD88) cDNA from Zhikong scallop Chlamys farreri. Fish andshellfish immunology23:614-623.
RAN, X. Z., X. F. BIAN, Y. C. JI, X. M. YAN, F. YANG et al.,2013White Spot Syndrome Virus IE1andWSV056modulate the G(1)/S Transition by binding to the host retinoblastoma protein. Journal ofvirology87:12576-12582.
RATTANACHAI, A., I. HIRONO, T. OHIRA, Y. TAKAHASHI and T. AOKI,2004Cloning of kuruma prawnMarsupenaeus japonicus crustin-like peptide cDNA and analysis of its expressiony. Fisheriesscience70:765-771.
REBL, A., T. GOLDAMMER, U. FISCHER, B. KOLLNER and H. M. SEYFERT,2009Characterization of twokey molecules of teleost innate immunity from rainbow trout (Oncorhynchus mykiss): MyD88andSAA. Veterinary immunology and immunopathology131:122-126.
ROBALINO, J., C. L. BROWDY, S. PRIOR, A. METZ, P. PARNELL et al.,2004Induction of antiviralimmunity by double stranded RNA in a marine invertebrate. Journal of virology78:10442-10448.
ROWLEY, A. F., and A. POWELL,2007Invertebrate immune systems specific, quasi specific, ornonspecific? Journal of immunology179:7209-7214.
RUSCANU, S., F. PASCALE, M. BOURGE, B. HEMATI, J. ELHMOUZI-YOUNES et al.,2012The Doublestranded RNA bluetongue virus induces type I interferon in plasmacytoid dendritic cells via aMyD88-dependent TLR7/8-independent signaling pathway. Journal of Vvirology86:5817-5828.
RUTSCHMANN, S., A. KILINC and D. FERRANDON,2002Cutting edge: The Toll pathway is required forresistance to Gram-positive bacterial infections in Drosophila. Journal of immunology168:1542-1546.
S DERH LL, I., E. BANGYEEKHUN, S. MAYO and K. S DERH LL,2003Hemocyte production andmaturation in an invertebrate animal; proliferation and gene expression in hematopoietic stemcells of Pacifastacus leniusculus. Developmental and comparative immunology27:661-672.
SABIN, L. R., S. L. HANNA and S. CHERRY,2010Innate antiviral immunity in Drosophila. Currentopinion in immunology22:4-9.
SALCEDO, R., C. CATAISSON, U. HASAN, S. H. YUSPA and G. TRINCHIERI,2013MyD88and itsdivergent toll in carcinogenesis. Trends in immunology34:379-389.
SAMUEL, C. E.,2001Antiviral actions of interferons. Clinical microbiology reviews14:778-809.
SEGOVIA, J., A. SABBAH, V. MGBEMENA, S.-Y. TSAI, T.-H. CHANG et al.,2012TLR2/MyD88/NF-kappa B pathway, reactive oxygen species, potassium efflux activatesNLRP3/ASC inflammasome during respiratory syncytial virus infection. Plos one7.
SHI, X. Z., R. R. ZHANG, Y. P. JIA, X. F. ZHAO, X. Q. YU et al.,2009Identification and molecularcharacterization of a Sp tzle-like protein from Chinese shrimp (Fenneropenaeus chinensis). Fishand shellfish immunology27:610-617.
SHI, Z., C. HUANG, J. ZHANG, D. CHEN and J. BONAMI,2000White spot syndrome virus (WSSV)experimental infection of the freshwater crayfish, Cherax quadricarinatus. Journal of fish diseases23:285-288.
SKJAEVELAND, I., D. B. ILIEV, G. STRANDSKOG and J. B. JORGENSEN,2009Identification andcharacterization of TLR8and MyD88homologs in Atlantic salmon (Salmo salar). Developmentaland comparative immunology33:1011-1017.
SOLDAINI, E., S. JOHN, S. MORO, J. BOLLENBACHER, U. SCHINDLER et al.,2000DNA binding siteselection of dimeric and tetrameric Stat5proteins reveals a large repertoire of divergent tetramericStat5a binding sites. Molecular and cellular biology20:389-401.
STARK, G. R., and J. E. DARNELL,2012The JAK/STAT pathway at twenty. Immunity36:503-514.
STENTIFORD, G. D., J. R. BONAMI and V. ALDAY-SANZ,2009A critical review of susceptibility ofcrustaceans to taura syndrome, yellow head disease and white spot disease and implications ofinclusion of these diseases in European legislation. Aquaculture291:1-17.
SUN, C., H. L. SHAO, X. W. ZHANG, X. F. ZHAO and J. X. WANG,2011Molecular cloning andexpression analysis of signal transducer and activator of transcription (STAT) from the Chinesewhite shrimp Fenneropenaeus chinensis. Molecular biology reports38:5313-5319.
SUN, Y. M., F. H. LI and J. H. XIANG,2013a Analysis on the dynamic changes of the amount of WSSVin Chinese shrimp Fenneropenaeus chinensis during infection. Aquaculture376–379:124-132.
SUN, Y. M., F. H. LI, Z. SUN, X. J. ZHANG, S. H. LI et al.,2014Transcriptome analysis of the initialstage of acute WSSV infection caused by temperature change. Plos one9.
SUN, Y. M., F. H. LI and J. H. XIANG,2013b Analysis on the dynamic changes of the amount of WSSVin Chinese shrimp Fenneropenaeus chinensis during infection. Aquaculture376:124-132.
TAHIRI-ALAOUI, A., L. P. SMITH, S. BAIGENT, L. KGOSANA, L. J. PETHERBRIDGE et al.,2009Identification of an intercistronic internal ribosome entry site in a Marek's disease virus immediateearly gene. Journal of virology83:5846-5853.
TAKANO, T., H. KONDO, I. HIRONO, T. SAITO-TAKI, M. ENDO et al.,2006Identification andcharacterization of a myeloid differentiation factor88(MyD88) cDNA and gene in Japaneseflounder, Paralichthys olivaceus. Developmental and comparative immunology30:807-816.
TAKAOKA, A., and H. YANAI,2006Interferon signalling network in innate defence. Cellularmicrobiology8:907-922.
TALREJA, J., M. H. KABIR, M. B. FILLA, D. J. STECHSCHULTE and K. N. DILEEPAN,2004Histamineinduces Toll-like receptor2and4expression in endothelial cells and enhances sensitivity toGram-positive and Gram-negative bacterial cell wall components. Immunology113:224-233.
TASSANAKAJON, A., K. SOMBOONWIWAT, P. SUPUNGUL and S. TANG,2013Discovery of immunemolecules and their crucial functions in shrimp immunity. Fish and shellfish immunology34:954-967.
TAUSZIG-DELAMASURE, S., H. BILAK, M. CAPOVILLA, J. A. HOFFMANN and J. L. IMLER,2002Drosophila MyD88is required for the response to fungal and Gram-positive bacterial infections.Nature immunology3:91-97.
THARNTADA, S., S. PONPRATEEP, K. SOMBOONWIWAT, H. P. LIU, I. SODERHALL et al.,2009Role ofanti-lipopolysaccharide factor from the black tiger shrimp, Penaeus monodon, in protection fromwhite spot syndrome virus infection. Journal of general virology90:1491-1498.
TYAGI, A., R. KHUSHIRAMANI, I. KARUNASAGAR and I. KARUNASAGAR,2007Antivibrio activity ofrecombinant lysozyme expressed from black tiger shrimp, Penaeus monodon. Aquaculture272:246-253.
VALANNE, S., J. H. WANG and M. RAMET,2011The Drosophila Toll signaling pathway. Journal ofimmunology186:649-656.
VAN DER SAR, A. M., O. W. STOCKHAMMER, C. VAN DER LAAN, H. P. SPAINK, W. BITTER et al.,2006aMyD88innate immune function in a zebrafish embryo infection model. Infection and immunity74:2436-2441.
VAN DER SAR, A. M., O. W. STOCKHAMMER, C. VAN DER LAAN, H. P. SPAINK, W. BITTER et al.,2006bMyD88innate immune function in a zebrafish embryo infection model. Infection and immunity74:2436.
VAN RIJ, R. P., M. C. SALEH, B. BERRY, C. FOO, A. HOUK et al.,2006The RNA silencing endonucleaseArgonaute2mediates specific antiviral immunity in Drosophila melanogaster. Genes anddevelopment20:2985-2995.
WANG, D., F. LI, S. LI, Y. CHI, R. WEN et al.,2012a An IkappaB homologue (FcCactus) in Chineseshrimp Fenneropenaeus chinensis. Developmental and comparative immunology39:352-362.
WANG, L. Y., F. H. LI, B. WANG and J. H. XIANG,2013A new shrimp peritrophin-like gene fromExopalaemon carinicauda involved in white spot syndrome virus (WSSV) infection. Fish andshellfish immunology35:840-846.
WANG, P., J. LIANG, Z. GU, D. WAN, S. WENG et al.,2012b Molecular cloning, characterization andexpression analysis of two novel Tolls (LvToll2and LvToll3) and three putative Sp tzle-like Tollligands (LvSpz1–3) from Litopenaeus vannamei. Developmental and comparative immunology36:359-371.
WANG, P., D. WAN, Z. GU, X. DENG, S. WENG et al.,2011A Litopenaeus vannamei tumor necrosisfactor receptor-associated factor6(TRAF6) responds to Vibrio alginolyticus and white spotsyndrome virus (WSSV) infection and activates antimicrobial peptide genes. Developmental andcomparative immunology35:105-114.
WANG, P. H., Z. H. GU, D. H. WAN, M. Y. ZHANG, S. P. WENG et al.,2011b The shrimp NF-kappa Bpathway is activated by White Spot Syndrome Virus (WSSV)449to facilitate the expression ofwsv069(ie1), wsv303and wsv371. Plos one6.
WANG, P. H., D. H. WAN, L. R. PANG, Z. H. GU, W. QIU et al.,2012c Molecular cloning,characterization and expression analysis of the tumor necrosis factor (TNF) superfamily gene,TNF receptor superfamily gene and lipopolysaccharide-induced TNF-alpha factor (LITAF) genefrom Litopenaeus vannamei. Developmental and comparative immunology36:39-50.
WARIS, G., K. W. HUH and A. SIDDIQUI,2001Mitochondrially associated hepatitis B virus X proteinconstitutively activates transcription factors STAT3and NF-kappa B via oxidative stress.Molecular and cellular biology21:7721-7730.
WARIS, G., J. TURKSON, T. HASSANEIN and A. SIDDIQUI,2005Hepatitis C virus (HCV) constitutivelyactivates STAT3via oxidative stress: role of STAT3in HCV replication. Journal of virology79:1569-1580.
WESCHE, H., W. J. HENZEL, W. SHILLINGLAW, S. LI and Z. D. CAO,1997MyD88: an adapter thatrecruits IRAK to the IL-1receptor complex. Immunity7:837-847.
WONGPRASERT, K., K. KHANOBDEE, S. S. GLUNUKARN, P. MEERATANA and B. WITHYACHUMNARNKUL,2003Time course and levels of apoptosis in various tissues of black tiger shrimp Penaeusmonodon infected with White Spot Syndrome Virus. Diseases of aquatic organisms55:3-10.
XI, Z. Y., J. L. RAMIREZ and G. DIMOPOULOS,2008The Aedes aegypti Toll pathway controls denguevirus infection. Plos pathogens4.
XU, X. A., Y. L. SUN and T. HOEY,1996Cooperative DNA binding and sequence selective recognitionconferred by the STAT amino terminal domain. Science273:794-797.
YAFEI DUAN, P. L., JITAO LI, JIAN LI1, YUN WANG AND PING CHEN,2014The responsive expression ofa chitinase gene in the ridgetail white prawn Exopalaemon carinicauda against Vibrioanguillarum and WSSV challenge. Cell Stress and Chaperones.
YAN, Y., H. C. CUI, J. G. WEI, Y. H. HUANG, X. H. HUANG et al.,2012Functional genomic studies onan immune-and antiviral-related gene of MyD88in orange-spotted grouper, Epinephelus coioides.Chinese science bulletin57:3277-3287.
YANG, C. J., J. Q. ZHANG, F. H. LI, H. M. MA, Q. L. ZHANG et al.,2008A toll receptor from Chineseshrimp Fenneropenaeus chinensis is responsive to Vibrio anguillarum infection. Fish and shellfishimmunology24:564-574.
YANG, L. S., Z. X. YIN, J. X. LIAO, X. D. HUANG, C. J. GUO et al.,2007A Toll receptor in shrimp.Molecular immunology44:1999-2008.
YAO, C. L., P. KONG, Z. Y. WANG, P. F. JI, X. D. LIU et al.,2009Molecular cloning and expression ofMyD88in large yellow croaker, Pseudosciaena crocea. Fish and shellfish immunology26:249-255.
YU, F. F., Y. B. ZHANG, T. K. LIU, Y. LIU, F. SUN et al.,2010Fish virus-induced interferon exertsantiviral function through Stat1pathway. Molecular immunology47:2330-2341.
YU, X. Q., H. GAN and M. R. KANOST,1999Immulectin, an inducible C-type lectin from an insect,Manduca sexta, stimulates activation of plasma prophenol oxidase. Insect biochemistry andmolecular biology29:585-597.
ZAMBON, R. A., M. NANDAKUMAR, V. N. VAKHARIA and L. P. WU,2005The Toll pathway is importantfor an antiviral response in Drosophila. Proceedings of the National Academy of Sciences of theUnited States of America102:7257-7262.
ZAMBON, R. A., V. N. VAKHARIA and L. P. WU,2006RNAi is an antiviral immune response against adsRNA virus in Drosophila melanogaster. Cellular microbiology8:880-889.
ZEIDLER, M. P., E. A. BACH and N. PERRIMON,2000The roles of the Drosophila JAK/STAT pathway.Oncogene19:2598-2606.
ZHAN, W. B., Y. H. WANG, J. L. FRYER, K. OKUBO, H. FUKUDA et al.,1999Production of monoclonalantibodies (MAbs) against White Spot Syndrome Virus (WSSV). Journal of aquatic animal health11:17-22.
ZHANG, S., C. Z. LI, H. YAN, W. QIU, Y. G. CHEN et al.,2012Identification and function of myeloiddifferentiation factor88(MyD88) in Litopenaeus vannamei. Plos one7.
ZHANG, Y., and J. GUI,2004Molecular characterization and IFN signal pathway analysis of Carassiusauratus CaSTAT1identified from the cultured cells in response to virus infection. Developmentaland comparative immunology28:211-227.
ZHAO, Z. Y., Z. X. YIN, S. P. WENG, H. J. GUAN, S. D. LI et al.,2007Profiling of differentiallyexpressed genes in hepatopancreas of White Spot Syndrome Virus-resistant shrimp (Litopenaeusvannamei) by suppression subtractive hybridisation. Fish and shellfish immunology22:520-534.
朱艳冰和杨丰,2005对虾白斑综合症病毒类锌指蛋白基因(wsv063、wsv069和wsv477)的表达和纯化.厦门大学学报(自然科学版):697-700.
李钫,2009对虾白斑综合症病毒极早期基因的研究,厦门大学.
易志刚,黄倢和李筠,2004WSSV结构、功能及基因组学研究概况.海洋水产研究:79-84.
相建海,2001海水养殖生物病害发生与控制.海洋出版社.
高溳,金春英和王蔚,2011对虾白斑综合症病毒IE1表达与鉴定.中国公共卫生:535-536.
吴月喜,2011对虾弧菌病诱因与防控措施.海洋与渔业10:54.
张志栋,战文斌,薛艳红和邢婧,2005凡纳滨对虾(Litopenaeus vannamei)感染白斑症病毒(WSSV)后大颗粒血细胞的变化.海洋与湖沼:67-71.
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