抗郁散对CUMS大鼠血清T_3、T_4、TSH的影响
|
摘要
目的:通过观察抗郁散对慢性轻度不可预见应激(CUMS)抑郁症模型大鼠行为学及血清T3、T4、TSH的影响,研究抗郁散对抑郁症动物模型HPT轴的影响,从而探讨抗郁散治疗抑郁症的药效学机制。
方法:采用多种长期慢性不可预见的轻度应激因子复制大鼠抑郁模型,设正常组、模型组、氟西汀组、抗郁散大、中、小剂量组,连续治疗21天,定期测定各组大鼠体重、糖水消耗量及行为学改变,并用放免法测定大鼠血清T3、T4、TSH含量的变化。
结果:与正常组比较,模型组大鼠体重下降、垂直和水平运动次数减少、糖水消耗量减少,有显著性差异(P<0.05),模型组大鼠血清T4的含量显著升高、TSH的含量升高,有显著性差异(P<0.05);T3的含量降低,无显著性差异(P>0.05)。与模型组相比,抗郁散大、小剂量组和氟西汀组,能使大鼠的行为学明显改善,恢复接近正常组水平,有显著性差异(P<0.05),血清T4值显著降低,TSH值降低,有显著性差异(P<0.05),而T3的值降低,无显著性差异(P>0.05);与模型组相比,抗郁散中剂量组能明显改善大鼠的行为特征,使大鼠血中清T4、TSH的含量显著降低、T3的含量明显升高,差异有显著性(P<0.05)。
结论:抗郁散可以改善大鼠行为学特征,使血清T3值含量升高、T4、TSH值含量降低,恢复接近正常组水平,其机制可能与抗郁散调节HPT轴功能紊乱有关,为抗郁散在临床上用于抑郁症的治疗提供理论基础。
Objective: Antidepressants scattered through the observation of unpredictable chronic mild stress rat model of depression behavior and serum T3, T4, TSH effects of antidepressants casual study of animal model of depression, the effects of HPTA in order to investigate the bulk Pharmacodynamics mechanism of antidepressants on depression.
Methods: Using a variety of chronic unpredictable mild stress model of depression in rats, normal group, model group, Fluoxetine group, antidepressants, scattered large, medium and small dose group, 21 days for treatment, periodic determination of each group rat body weight, sugar consumption and behavior change, and RIA determination of serum T3, T4, TSH concentration change.
Results: Compared with the normal group, model group rats lost weight, vertical and horizontal reduction in the number of sports, to reduce consumption of dessert, there is significant difference (P<0.05), model group, serum T4 levels were significantly increased, TSH levels rise, there is significant difference (P<0.05); T3 levels decreased, no significant difference(P>0.05).Compared with model group, antidepressants scattered large ,medium and small dose group and the Fluoxetine group, can study the behavior of rats markedly improved, the restoration of nearly normal levels, there is significant difference (P<0.05), serum T4 was significantly lowering, TSH values decrease, there is significant difference (P<0.05), and T3 of the values decrease, no significant difference (P>0.05); Compared with model group, antidepressants Powder dosage group can obviously improve the rat's behavioral characteristics, so that blood clearance of T4, TSH levels significantly decreased, T3 levels increased significantly, there was a significant difference (P <0.05 ).
Conclusions: Antidepressants can improve the dispersion characteristics of rat behavior, so that the value of serum T3 was increased, T4, TSH values decreased to restore near normal levels, the mechanism of antidepressants may be associated with regulation of HPTA scattered dysfunction related to antidepressants scattered on clinical treatment for depression and provide a theoretical foundation.
方法:采用多种长期慢性不可预见的轻度应激因子复制大鼠抑郁模型,设正常组、模型组、氟西汀组、抗郁散大、中、小剂量组,连续治疗21天,定期测定各组大鼠体重、糖水消耗量及行为学改变,并用放免法测定大鼠血清T3、T4、TSH含量的变化。
结果:与正常组比较,模型组大鼠体重下降、垂直和水平运动次数减少、糖水消耗量减少,有显著性差异(P<0.05),模型组大鼠血清T4的含量显著升高、TSH的含量升高,有显著性差异(P<0.05);T3的含量降低,无显著性差异(P>0.05)。与模型组相比,抗郁散大、小剂量组和氟西汀组,能使大鼠的行为学明显改善,恢复接近正常组水平,有显著性差异(P<0.05),血清T4值显著降低,TSH值降低,有显著性差异(P<0.05),而T3的值降低,无显著性差异(P>0.05);与模型组相比,抗郁散中剂量组能明显改善大鼠的行为特征,使大鼠血中清T4、TSH的含量显著降低、T3的含量明显升高,差异有显著性(P<0.05)。
结论:抗郁散可以改善大鼠行为学特征,使血清T3值含量升高、T4、TSH值含量降低,恢复接近正常组水平,其机制可能与抗郁散调节HPT轴功能紊乱有关,为抗郁散在临床上用于抑郁症的治疗提供理论基础。
Objective: Antidepressants scattered through the observation of unpredictable chronic mild stress rat model of depression behavior and serum T3, T4, TSH effects of antidepressants casual study of animal model of depression, the effects of HPTA in order to investigate the bulk Pharmacodynamics mechanism of antidepressants on depression.
Methods: Using a variety of chronic unpredictable mild stress model of depression in rats, normal group, model group, Fluoxetine group, antidepressants, scattered large, medium and small dose group, 21 days for treatment, periodic determination of each group rat body weight, sugar consumption and behavior change, and RIA determination of serum T3, T4, TSH concentration change.
Results: Compared with the normal group, model group rats lost weight, vertical and horizontal reduction in the number of sports, to reduce consumption of dessert, there is significant difference (P<0.05), model group, serum T4 levels were significantly increased, TSH levels rise, there is significant difference (P<0.05); T3 levels decreased, no significant difference(P>0.05).Compared with model group, antidepressants scattered large ,medium and small dose group and the Fluoxetine group, can study the behavior of rats markedly improved, the restoration of nearly normal levels, there is significant difference (P<0.05), serum T4 was significantly lowering, TSH values decrease, there is significant difference (P<0.05), and T3 of the values decrease, no significant difference (P>0.05); Compared with model group, antidepressants Powder dosage group can obviously improve the rat's behavioral characteristics, so that blood clearance of T4, TSH levels significantly decreased, T3 levels increased significantly, there was a significant difference (P <0.05 ).
Conclusions: Antidepressants can improve the dispersion characteristics of rat behavior, so that the value of serum T3 was increased, T4, TSH values decreased to restore near normal levels, the mechanism of antidepressants may be associated with regulation of HPTA scattered dysfunction related to antidepressants scattered on clinical treatment for depression and provide a theoretical foundation.
引文
[1]裴瑾,万德光.中医药治疗抑郁症的现状与展望[J].中国中医药信息杂志,2002,9(7):75
[2]邱艳明,时宇静.电针印堂、百会对获得性无助大鼠不同脑区内单胺类神经递质的影响.北京中医药大学学报,2002,25(6):54-56
[3]杨进,谢忠礼.菖欢Ⅰ号对大鼠抑郁症模型脑内单胺类神经递质的影响.南京中医药大学学报(自然科学版),2001,17(5):294-298
[4]张中启,黄世杰.巴戟天寡糖对鼠强迫游泳和获得性无助抑郁模型的影响.中国药理学与毒理学杂志,2001,15(4):262-265
[5]殷大奎,消除偏见,勇于关爱,积极做好精神卫生工作.精神康复报,2001,4(2)
[6]willner P.validity,reliability and utility of the chronic mild stress model of depression:a 10-yeal review and evaluation[J],Psychophamacology(Berl), 1997,134(4):319-329
[7]徐舒云,卞如濂药理实验方法学[M]第三版人民卫生出版社2006
[8]张孝娟,黄小玲中医临床心理学[M]中国医药科技出版社2006
[9]顾牛范,李华芳,舒良,等.圣约翰草提取物治疗轻中度抑郁症的多中心临床研究[J].中国临床药学杂志,2001,10(5):271.
[10]瞿发林,张理义,李宁,等.贯叶连翘抗抑郁作用机制的演技也进展[J].中国神经精神疾病杂志,2003,12(1):80
[11]粱建辉,舒良,罗质璞巴戟天水提物治疗抑郁症临床疗效初探[J].中国中药杂志,2002,27(1):75-78
[12]蒋建章,车丰远,刘翠菊,等.银杏叶片治疗血管性抑郁症的临床观察[J].中国中西医结合杂志,2003,23(5):388-399
[13]李宗友.阔叶缬草的抗抑郁成分[J]国外医学·中医中药分册,1996,18(2):47—48
[14]景显芳.金丝桃浸膏治疗抑郁症的资料在增多[J].国外药讯,1997,3:35～37
[15]秦路平.积雪草挥发油成分分析及抗抑郁作用研究[J].第二军医大学学报,2002,23(11)1224-1226
[16]王斌,刘天培.柴胡皂苷加强氟西汀在强迫游泳模型上的抗抑郁作用[J].中草药,1997,28(12):729-731
[17]李亚明,陈红梅.石菖蒲对行为绝望的动物抑郁模型的抗抑郁作用[J].中药材,2001,24(1):40-41
[18]翁深红,程自立,王高华.刺五加胶囊治疗抑郁症的临床对照研究[J].湖北中医杂志, 2001,23(6):8-9
[19]丁文娟,徐国祥.中医治疗抑郁症40例临床分析[J].江苏中医,1994,15(4):18
[20]吴鉴明.加味甘麦大枣汤抗抑郁疗效的对照研究[J].中国临床医生, 2002, 30 (1) : 18-19
[21]张有志,聂惠民,张德昌,等.柴胡加龙骨牡蛎汤等经方治疗抑郁症的动物行为学研究[J].中国中医基础医学杂志,2001,7(7):30-32
[22]傅强,马世平,瞿融.半夏厚朴汤抗抑郁作用的研究[J].中国药科大学学报, 2002,33(6):514-517
[23]李建梅,杨澄,张伟云,等.半夏厚朴汤醇提物对大鼠慢性抑郁模型的影响[J].中国中药杂志, 2003,28(1): 55-58
[24]曹忠义,高颂.逍遥散加味治疗脑卒中后抑郁症69例[J].中医研究,2000,13(3):45
[25]张美曾,张秋英,崔广志.逍遥散治疗抑郁性神经症临床研究[J].山东中医药大学学报,1998 ,22(1):34—37
[26]吴丽丽,徐志伟,严灿,等.逍遥散和丹栀逍遥散抗抑郁作用的实验研究[J].中医研究, 2003,16(3): 14-15
[27]董莉,吕嵘,洪岩,等.补肾解郁清心方对更年期抑郁症模型大鼠HPO轴及单胺类神经递质的影响[J].上海中医药大学学报, 2003,17(2): 38-41
[28]卢峻,时宇静,图娅.解郁汤对小鼠强迫游泳的作用机理研究[J].中国实验方剂学杂志, 2003,9(4): 49-50
[29]薛征,关建红.脾肾二助丸抗抑郁的实验研究[J].中西医结合心脑血管杂志, 2003,1(12):714-715
[30]马尔蒂尼(Marini,L)主编,甲状腺轴[M]科学出版社.2008,4
[31]沈渔邨主编,精神病学[M]第4版人民卫生出版社.1980,10
[32]王学义,李文通,权长庚.情感性精神障碍治疗前后血清甲状腺激素水平对照观察[J].中华神经精神疾病杂志, 1998,31(1):19-22
[33]李慧璟疏肝解郁饮对抑郁大鼠行为及血浆ACTH、血清T3、T4、TSH、rT3的影响[D]吉林大学硕士学位论文
[34]Francis S, Greemspan MD, Gordon J, et al.基础与临床内分泌学[M].世界图书出版西安公司, 2001,5第一版: 205-223
[35]陈文泽,王宏地,高祖华.反复发作抑郁症甲状腺激素水平对照观察[J].中华神经精神疾病杂志, 1999,25(4):242-243
[36]吕俊华,库宝善.电刺激小鼠角膜诱发的不动状态:一种新的抗抑郁药物研究动物模型[J].北京医科大学学报, 1990,22(5):354 -356
[37]官志忠. TSH的调节及病理生理[J].国外医学内分泌学分册,1987,7:4-6
[38]裘国花.应激状态时血甲状腺激素和皮质醇的变化[J].临床内科杂志,2003,20(5): 233
[39]甲状腺激素增强舍曲林治疗严重抑郁症刘晓获孙瑛译编
[1]Murray CJ,Lopez AD.The global burden of disease:a comprehensive.assessment of mortality and disability from diseases,injuries,and risk factors in 1990 and projected to 2020 Cambridge(USA):Harrard University press. 1996:417-435
[2]殷大奎,消除偏见,勇于关爱,积极做好精神卫生工作.精神康复报,2001—04—07(2)
[3]HowlandRH ,GewirtzGR ,GuscottR.符少剑编译.甲状腺功能障碍与难治性抑郁症[J] .国外医学精神病学分册,1994 ,21 (1) :40～43
[4]Gunnarsson T, et al:Neuropsychobiology,2001;43(2):70-74.
[5]Brownlie BE, et al:Endocrinol.2000;142(5):438-444.
[6]黄泽智陈欢抑郁症患者甲状腺激素水平变化的临床探讨[J]中国现代医学杂志2007,17(18):2262-2264
[7]莫煊党海红抑郁症患者细胞因子与甲状腺激素的研究[J]中国健康心理学杂2008,16(4):431-432
[8]Usselman DL,et al:Br J Psychiatry,1996;30:l23—128.
[9]Sullivan PF, et al: Acta Psyciatr Scand. 1997; 95: 370—378.
[10]Kraus RP,et al:J Clin Psychiatry,1997;58 (6):266—270.
[11]陈瑶秦路平等,积雪草总苷对抑郁症大鼠神经内分泌功能的影响,第二军医大学学报, 2002,23(11)1224-1226
[12]李慧璟疏肝解郁饮对抑郁大鼠行为及血浆ACTH、血清T3、T4、TSH、rT3的影响[D]吉林大学硕士学位论文
[13]Fava M, et al:Acta Psyehiatr Scand. 1992;86:42—45.
[14]Moreau X, et al: Prog Neuropsyehopharmacol Biol Psychiatry, 2000;24(1);23—25.
[2]邱艳明,时宇静.电针印堂、百会对获得性无助大鼠不同脑区内单胺类神经递质的影响.北京中医药大学学报,2002,25(6):54-56
[3]杨进,谢忠礼.菖欢Ⅰ号对大鼠抑郁症模型脑内单胺类神经递质的影响.南京中医药大学学报(自然科学版),2001,17(5):294-298
[4]张中启,黄世杰.巴戟天寡糖对鼠强迫游泳和获得性无助抑郁模型的影响.中国药理学与毒理学杂志,2001,15(4):262-265
[5]殷大奎,消除偏见,勇于关爱,积极做好精神卫生工作.精神康复报,2001,4(2)
[6]willner P.validity,reliability and utility of the chronic mild stress model of depression:a 10-yeal review and evaluation[J],Psychophamacology(Berl), 1997,134(4):319-329
[7]徐舒云,卞如濂药理实验方法学[M]第三版人民卫生出版社2006
[8]张孝娟,黄小玲中医临床心理学[M]中国医药科技出版社2006
[9]顾牛范,李华芳,舒良,等.圣约翰草提取物治疗轻中度抑郁症的多中心临床研究[J].中国临床药学杂志,2001,10(5):271.
[10]瞿发林,张理义,李宁,等.贯叶连翘抗抑郁作用机制的演技也进展[J].中国神经精神疾病杂志,2003,12(1):80
[11]粱建辉,舒良,罗质璞巴戟天水提物治疗抑郁症临床疗效初探[J].中国中药杂志,2002,27(1):75-78
[12]蒋建章,车丰远,刘翠菊,等.银杏叶片治疗血管性抑郁症的临床观察[J].中国中西医结合杂志,2003,23(5):388-399
[13]李宗友.阔叶缬草的抗抑郁成分[J]国外医学·中医中药分册,1996,18(2):47—48
[14]景显芳.金丝桃浸膏治疗抑郁症的资料在增多[J].国外药讯,1997,3:35～37
[15]秦路平.积雪草挥发油成分分析及抗抑郁作用研究[J].第二军医大学学报,2002,23(11)1224-1226
[16]王斌,刘天培.柴胡皂苷加强氟西汀在强迫游泳模型上的抗抑郁作用[J].中草药,1997,28(12):729-731
[17]李亚明,陈红梅.石菖蒲对行为绝望的动物抑郁模型的抗抑郁作用[J].中药材,2001,24(1):40-41
[18]翁深红,程自立,王高华.刺五加胶囊治疗抑郁症的临床对照研究[J].湖北中医杂志, 2001,23(6):8-9
[19]丁文娟,徐国祥.中医治疗抑郁症40例临床分析[J].江苏中医,1994,15(4):18
[20]吴鉴明.加味甘麦大枣汤抗抑郁疗效的对照研究[J].中国临床医生, 2002, 30 (1) : 18-19
[21]张有志,聂惠民,张德昌,等.柴胡加龙骨牡蛎汤等经方治疗抑郁症的动物行为学研究[J].中国中医基础医学杂志,2001,7(7):30-32
[22]傅强,马世平,瞿融.半夏厚朴汤抗抑郁作用的研究[J].中国药科大学学报, 2002,33(6):514-517
[23]李建梅,杨澄,张伟云,等.半夏厚朴汤醇提物对大鼠慢性抑郁模型的影响[J].中国中药杂志, 2003,28(1): 55-58
[24]曹忠义,高颂.逍遥散加味治疗脑卒中后抑郁症69例[J].中医研究,2000,13(3):45
[25]张美曾,张秋英,崔广志.逍遥散治疗抑郁性神经症临床研究[J].山东中医药大学学报,1998 ,22(1):34—37
[26]吴丽丽,徐志伟,严灿,等.逍遥散和丹栀逍遥散抗抑郁作用的实验研究[J].中医研究, 2003,16(3): 14-15
[27]董莉,吕嵘,洪岩,等.补肾解郁清心方对更年期抑郁症模型大鼠HPO轴及单胺类神经递质的影响[J].上海中医药大学学报, 2003,17(2): 38-41
[28]卢峻,时宇静,图娅.解郁汤对小鼠强迫游泳的作用机理研究[J].中国实验方剂学杂志, 2003,9(4): 49-50
[29]薛征,关建红.脾肾二助丸抗抑郁的实验研究[J].中西医结合心脑血管杂志, 2003,1(12):714-715
[30]马尔蒂尼(Marini,L)主编,甲状腺轴[M]科学出版社.2008,4
[31]沈渔邨主编,精神病学[M]第4版人民卫生出版社.1980,10
[32]王学义,李文通,权长庚.情感性精神障碍治疗前后血清甲状腺激素水平对照观察[J].中华神经精神疾病杂志, 1998,31(1):19-22
[33]李慧璟疏肝解郁饮对抑郁大鼠行为及血浆ACTH、血清T3、T4、TSH、rT3的影响[D]吉林大学硕士学位论文
[34]Francis S, Greemspan MD, Gordon J, et al.基础与临床内分泌学[M].世界图书出版西安公司, 2001,5第一版: 205-223
[35]陈文泽,王宏地,高祖华.反复发作抑郁症甲状腺激素水平对照观察[J].中华神经精神疾病杂志, 1999,25(4):242-243
[36]吕俊华,库宝善.电刺激小鼠角膜诱发的不动状态:一种新的抗抑郁药物研究动物模型[J].北京医科大学学报, 1990,22(5):354 -356
[37]官志忠. TSH的调节及病理生理[J].国外医学内分泌学分册,1987,7:4-6
[38]裘国花.应激状态时血甲状腺激素和皮质醇的变化[J].临床内科杂志,2003,20(5): 233
[39]甲状腺激素增强舍曲林治疗严重抑郁症刘晓获孙瑛译编
[1]Murray CJ,Lopez AD.The global burden of disease:a comprehensive.assessment of mortality and disability from diseases,injuries,and risk factors in 1990 and projected to 2020 Cambridge(USA):Harrard University press. 1996:417-435
[2]殷大奎,消除偏见,勇于关爱,积极做好精神卫生工作.精神康复报,2001—04—07(2)
[3]HowlandRH ,GewirtzGR ,GuscottR.符少剑编译.甲状腺功能障碍与难治性抑郁症[J] .国外医学精神病学分册,1994 ,21 (1) :40～43
[4]Gunnarsson T, et al:Neuropsychobiology,2001;43(2):70-74.
[5]Brownlie BE, et al:Endocrinol.2000;142(5):438-444.
[6]黄泽智陈欢抑郁症患者甲状腺激素水平变化的临床探讨[J]中国现代医学杂志2007,17(18):2262-2264
[7]莫煊党海红抑郁症患者细胞因子与甲状腺激素的研究[J]中国健康心理学杂2008,16(4):431-432
[8]Usselman DL,et al:Br J Psychiatry,1996;30:l23—128.
[9]Sullivan PF, et al: Acta Psyciatr Scand. 1997; 95: 370—378.
[10]Kraus RP,et al:J Clin Psychiatry,1997;58 (6):266—270.
[11]陈瑶秦路平等,积雪草总苷对抑郁症大鼠神经内分泌功能的影响,第二军医大学学报, 2002,23(11)1224-1226
[12]李慧璟疏肝解郁饮对抑郁大鼠行为及血浆ACTH、血清T3、T4、TSH、rT3的影响[D]吉林大学硕士学位论文
[13]Fava M, et al:Acta Psyehiatr Scand. 1992;86:42—45.
[14]Moreau X, et al: Prog Neuropsyehopharmacol Biol Psychiatry, 2000;24(1);23—25.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |