葡聚糖微结构的聚乙烯醇凝胶敷料的制备
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摘要
本文设计在聚乙烯醇(PVA)宏观凝胶的网络中嵌入葡聚糖(Dex)微凝胶,把微凝胶的快速响应性与宏观凝胶的高机械强度有效结合起来,形成含Dex微结构的PVA水凝胶,实现了微凝胶和大凝胶的优势互补,预期可以形成一种具有优良溶胀性能和机械性能的凝胶材料。其预期的高机械性能可保持敷料形状的完整性,不会碎裂,对外界起到屏障作用,抑制病菌入侵感染；同时其优良的溶胀性能可快速充分地吸收渗出液,保持创面的清爽,吸收渗液后不会粘贴伤口,形成湿润闭密环境,可加速上皮细胞生长,促进创面的愈合。同时拟建在凝胶中包埋伤口消炎药苯妥英钠,小分子药物缓慢释放持续长久地发挥药效,期望可以更快速优效地促进伤口的愈合,形成一个全面功效的优良的新型高分子敷料
首先,采用微波协助冻融法是制备PVA宏观凝胶的关键,通过对循环次数、反应液浓度、冷冻时间和微波时间的考察,进行溶胀性能和力学性能检测,实现一个凝胶的综合性能最优化的制备方案
其次,设计了几种不同结构的PVA凝胶,对其进行溶胀性能和机械强度测试,选取综合性能最优的凝胶结构模型,为后期包埋药物奠定基础。
最后,对选定的凝胶材料包埋消炎药苯妥英钠,考察了包埋和吸附的方式对药物释放的影响,并优化了工艺条件,模拟伤口渗出液的条件进行药物缓释测试,同时进行了药理实验以评价凝胶敷料的实际药效。
PVA/Dex BHMs Hydrogel is designed for wound dressing,whose upstanding cs-aa-hema and mechanical property is expected.The mechanical property is used for making the wound dressing complete to restrain the wound infected.The cs-aa-hema is used for making the dressing absorb blood quickly to keep the wound clear.Furthermore,the dressing can provide a moist environment to accelerate epithelium grow up.In addition,dilantin sodium is embedded in the hydrogel to strengthen its glyph of swiftmend.
The wavelet and freezing method for making the hydrogel is very important,so the process conditions can be investigated,such as the temperature,the RHF concentration,the time and so on.According to checking its cs-aa-hema and mechanical property,the best preparation method is set up.
Some different kinds of hydrogels are designed to prepared,whose property is compared to get a best kind of hydrogel.It is based for the preparation of the wound dressing.
At last,dilantin sodium is embedded in the hydrogel,according to check its releasing function,whose glyph of swiftmend can be tested first.Also,pharmacological test is used to evaluate its function further.
首先,采用微波协助冻融法是制备PVA宏观凝胶的关键,通过对循环次数、反应液浓度、冷冻时间和微波时间的考察,进行溶胀性能和力学性能检测,实现一个凝胶的综合性能最优化的制备方案
其次,设计了几种不同结构的PVA凝胶,对其进行溶胀性能和机械强度测试,选取综合性能最优的凝胶结构模型,为后期包埋药物奠定基础。
最后,对选定的凝胶材料包埋消炎药苯妥英钠,考察了包埋和吸附的方式对药物释放的影响,并优化了工艺条件,模拟伤口渗出液的条件进行药物缓释测试,同时进行了药理实验以评价凝胶敷料的实际药效。
PVA/Dex BHMs Hydrogel is designed for wound dressing,whose upstanding cs-aa-hema and mechanical property is expected.The mechanical property is used for making the wound dressing complete to restrain the wound infected.The cs-aa-hema is used for making the dressing absorb blood quickly to keep the wound clear.Furthermore,the dressing can provide a moist environment to accelerate epithelium grow up.In addition,dilantin sodium is embedded in the hydrogel to strengthen its glyph of swiftmend.
The wavelet and freezing method for making the hydrogel is very important,so the process conditions can be investigated,such as the temperature,the RHF concentration,the time and so on.According to checking its cs-aa-hema and mechanical property,the best preparation method is set up.
Some different kinds of hydrogels are designed to prepared,whose property is compared to get a best kind of hydrogel.It is based for the preparation of the wound dressing.
At last,dilantin sodium is embedded in the hydrogel,according to check its releasing function,whose glyph of swiftmend can be tested first.Also,pharmacological test is used to evaluate its function further.
引文
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[3]Queen D,Gaylor JDS. Biomaterials,1987,8:367
[4]Purna Sai K,Mary Babu. Burns,2000,26:54
[5]Janusz M,Rosiak. Nuclear Instruments and Methods in Physics Research B,1999, 151:56
[6]潘华先,程定超.国外医学生物医学工程分册,1991,17：207-208
[7]Shah SS, Kulkarni MG, Mashelkar RA, et al.J Controlled Release,1991,15(2): 121 - 132.
[8]Jennifer S.L., Babur Z.C., et al. Polymer,1998,39:1207-1210.
[9]廖叶华,董汝秀,范正.高分子学报,1993,6：672—677.
[10]王昌华,卢英先,曹维孝.高分子学报,1998,2：236—239.
[11]刘晓华,王晓工,刘德山.高分子学报[J],2000,10：126.
[12]Wu X S, Hoffman A S, Yagger P. J Polym Sci, Part A:Polym Chem[J],1992, 30:212122129.
[13]Zhang X Z, Zhuo R X. Eur Polym J[J],2000,36:230122303.
[14]Chen J, Park H, Park K. J Biomed Mater Res[J],1999,44(1):53262.
[15]Chen J, Park K. J Control Release[J],2000,65:73282.
[16]Chen J, Park K. Carbohydrate Polymer[J],2000,41:2592268.
[17]Yoshida R, Uchida K, et al. Nature[J],1995,374:2402242.
[18]Kaneko Y, Nakamura S, Kiyotaka Sakai, et al.Macromolecules[J],1998,31: 609926105.
[19]Ju H K, Kim S Y, Lee YM. Polymer[J],2001,42:685126857.
[20]Jeong B, Bae Y H, Kim S W. Macromolecules[J],1999,32:706427069.
[21]Bezemer JM, Radersma R, Grijipma DW, et al. J ControlRelease[J],2000,64: 1792192.
[22]Lin H H, Cheng Y L. Macromolecules[J],2001,34:371023715.
[23]Stenekes R J H, Talsma H, Hennink W E. Biomater[J],2001,22:189121898.
[24]Mathur, Arvind M, Hammonds, et al. J Control Release[J],1998,54: 1772184.
[25]Tang A, Wang C, Stewart R J, et al. J Control Release[J],2001,72:57270.
[26]Kwon I C, BaeY H, Kim S W. Nature[J],1991,354:2912293.
[27]Osada Y, Okuzaki H, Hori H. Nature[J],1992,355:2422244.
[28]Tanaka T. Scientific American[J],1981,244(1):1032111.
[29]Pelton R H, Chibante P. Colloid Surface[J],1986,20:2472256.
[30]Hoare T, Pelton R. Macromolecules [J],2004,37:254422550.
[31]Jones C D, Lyon L A. Macromolecules[J],2003,36:198821993.
[32]CaiW S, Gupta R B. J Appl Polym Sci[J],2002,83:1692178.
[33]潘育松,熊党生.高分子材料科学与工程,2007,23：229-231
[34]庄饭风,朱仲棋.高分子材料科学与工程.1999,15：145—47
[35]Cavalieri F, Chiessi E. Biomacromolecules,2008,9:1967-1973.
[36]Bodugoz-Senturk H, Macias CE. Biomaterials,2009,30:589-596.
[37]Yao Ri-Sheng, You Qi-Dong. Journal of Applied Polymer Science,2009, 111:358-362.
[38]Yang Xiaomin, Liu Qi. Carbohydrate Polymers,2008,73:401-408.
[39]Suong-Hyn Hyon,W-I Cha.Pollymer Bulletin,1989,22:119-122
[40]《肠膜状明串珠菌》[M]北京：中国医学科学院血液研究所,(1985),2-10.
[41]赵乃才,张克义主编,《临床药物不良反应大典》,辽宁科学技术出版社(1998) 91-97.
[42]《中华人民共和国药典》二部[S]2000版,111-115.
[43]S. Mitra,U.Gaur,P.C.Ghosh, et al. Journal of Controlled Release,74 (2001) 317-323.
[44]严忠勤,钟裕国,温玉明.中国药物化学杂志,1999,9：240-244
[45]F. Levi-Schaffer, A. Bernstein, A. Meshorer, R. Arnon.Cancer Treat. Rep,66 (1982) 107-114.
[46]姚日生,高文霞,邓胜松.精细化工,2004,32：27
[47]Lene Simosen, Lars Hovgaard, Per Brobech Mortensen.European Journal of Pharmaccutical Sciences,1995,3:329-337.
[48]Lars Hovgaard, Helle Brondsted. Journal of controlled released release,1995,36:156-166.
[49]R.J.H. Stenekes, A.E.Loebis, C.M. Fernandes. International Journal of Pharmaceutics,2001,214:17-20
[50]王锡华.实用医学杂志,2000,16：881.
[51]向代琼,杨蓉.中华护理杂志,1997,31：652-2653.
[52]刘碧文,宋翔.中华皮肤科杂志,1 997,30：57-58.
[53]张玉平,温守东,刘云鹤.化工进展,2007,26：834-840
[54]易昌凤,邓字巍,徐祖顺.高分子通报,2004,2：30-35
[55]Yokoyama F, Masada I. Colloid & Polymer Sci,1986,264:595-601.
[56]Lai Zini,Cui Yingde,Wu Xingxiang.Journal of Chemical Industry and Engineering,2008,59:3145.
[57]徐玉福,姚日生.化工学报,2007,58：3098.
[58]秦爱香,吕满庚.高分子材料科学与工程,2006,5：55.
[59]蔡勇,杨荣杰.高分子材料科学与工程,2007,4：102.
[60]Battersby J., Clark R., Hancock W.. Journal of controlled release,1996,1:143-156.
[61]徐风华,蒋雪涛,杨全胜,主皓.中国药学杂志,2000,35：455-457.
[62]Ahma S.,Tester R.F.,Corbett A.,Karkalas J.Carbohydrate Research,2006;34:2694-2701.
[63]徐玉福,姚日生.高分子材料科学与工程,2008,1：6.
[64]廖工铁.靶向给药制剂,第一版.成都：四川科学技术出版社,1997：113-220.
[65]Alexander D,Gammage D.Analysis of strike-though contamination in saturated sterile dressings.Clin Nurs Res,1992,1:28-34.
[66]潘育松,熊党生,陈晓林,聚乙烯醇水凝胶的制备及性能,高分子材料科学与工程,2007,23(6)：228-231.
[67]黄晓兵,张熙,代 华,杨万庆,PVA/P(AA-AM)复合水凝胶的制备及性能,高分子材料科学与工程,2008，24(6)：30-33.
[68]张先正 卓仁禧,温度敏感聚(N-异丙基丙烯酰胺)/聚(乙烯醇)水凝胶的制备及性能研究,高等学校化学学报,2000,21(11)：1776～1778.
[69]关静,高萍,黄姝杰,李志宏,武继民,张西正,聚乙烯醇水凝胶的生物相容性评价,中国组织工程研究与临床康复,2009,13(12)：2227-2231.
[70]叶春婷,陈鸿辉,邹海燕,潘政军,彭燕豪,聚乙烯醇-胶原创伤敷料的研制,生物医学工程学杂志,2008,25(3)：604-606.
[71]Schmedlen R. H., Masters K. S., West J. L, Photocrosslinkable polyvinyl alcohol hydrogels that can be modified with cell adhesion peptides for use in tissue engineering, Biomaterials,2002,23:4325-4332.
[72]Qiang Xu, Fengmin Lu and Jun Nie, Electrospun Water-Soluble Carboxyethyl Chitosan/Poly(vinyl alcohol) Nanofibrous Membrane as Potential Wound Dressing for Skin Regeneration, Yingshan Zhou, Dongzhi Yang, Xiangmei Chen, Biomacromolecules,2008,9 (1):349-354.
[73]Mei-Hua Huang, Ming-Chien Yang, Evaluation of glucan/poly(vinyl alcohol) blend wound dressing using rat models, International Journal of Pharmaceutics, Volume 346, Issues 1-2,4 January 2008, Pages 38-46.
[74]Jong Oh Kim, Jung Kil Park, Jeong Hoon Kim, et al.,Development of polyvinyl alcohol-sodium alginate gel-matrix-based wound dressing system containing nitrofurazone, International Journal of Pharmaceutics, Volume 359, Issues 1-2, 9 July 2008, Pages 79-86.
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