电项针对脑缺血再灌注大鼠脑保护作用的实验研究
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摘要
中风是常见病、多发病,病死率、致残率极高,而缺血性中风约占75%,且有逐年增加的趋势。随着溶栓疗法的应用,再灌注损伤的问题越来越受到重视。研究表明,脑缺血再灌注损伤(CIR)与神经细胞凋亡、炎症反应、钙超载、氧自由基的产生、兴奋性氨基酸的毒性作用等密切相关。因此预防和治疗缺血性中风,降低它的发病率和致残率,已成为当前迫切需要解决的问题。本课题通过研究电项针对脑缺血再灌注损伤的脑保护作用及其作用机理,为缺血性脑血管病的病理生理机制的研究和治疗方法的选择提供实验依据。
目的:观察大鼠CIR后缺血半暗区ICAM-1和凋亡相关基因表达的变化规律及电项针对其的影响,探讨电项针治疗脑梗死可能的作用机制。
方法:采用栓线法制备局灶性脑缺血动物模型,将Wistar大鼠随机分为假手术组(A)、模型组(B)、电体针组(C)及电项针组(D),各组随机分成12h、1d、3d、5d四个时间段,对大鼠进行实验观察。(1)参照Zea-Longa的5级评分标准对其神经功能障碍进行评分;(2)通过HE染色观察病理形态学变化;(3)电镜观察脑组织超微结构的变化:(4)通过TUNEL法检测细胞凋亡;(5)应用免疫组织化学方法检测缺血半暗区Bcl-2、Fas-1、ICAM-1的蛋白表达;(6)应用原位杂交技术检测缺血半暗区Fas-1 mRNA和ICAM-1 mRNA的表达。
结果:
(1)模型组脑缺血再灌注后出现明显神经功能缺损,与假手术组比较差异显著(P＜0.01),证明脑缺血再灌注损伤实验模型成功;电体针组、电项针组与模型组比较差异显著(p＜0.01,p＜0.05);电项针组与电体针组比较亦有显著性差异(p＜0.05)。说明电项针对神经功能缺损的恢复有明显的促进作用。
(2)HE染色显示:与模型组比较电体针组和电项针组缺血半暗区神经细胞肿胀减轻,胶质细胞增生,神经元数量和新生毛细血管增多;电项针组较电体针组明显。
(3)电镜观察显示:与模型组比较电体针组和电项针组神经细胞超微结构的变化明显改善,电项针组优于电体针组。
(4)电体针组和电项针组均有很好地抑制细胞凋亡的作用,与模型组比较均有显著性差异(p＜0.05,p＜0.01);电体针组与电项针组3d和5d时比较有显著性差异(p＜0.05)。可见,电项针组抑制细胞凋亡作用最优。
(5)电体针组和电项针组与模型组比较,可明显提高Bcl-2蛋白表达水平,并在早期抑制Fas-1蛋白及其mRNA的表达、ICAM-1蛋白及其mRNA的表达(p＜0.05,p＜0.01)。同时间点电项针组与电体针组比较有显著性差异(p＜0.05)。
结论:
(1)电项针可有效促进脑缺血再灌注大鼠神经功能的恢复。
(2)电项针可促进缺血半暗带区胶质细胞和毛细血管增生,减轻神经元损伤,改善神经细胞的超微结构。
(3)电项针有较好地抑制细胞凋亡的作用,可促进Bcl-2蛋白表达,抑制Fas-1蛋白及其mRNA的表达,表明其抑制神经细胞凋亡的作用与调控Bcl-2蛋白和Fas-1蛋白及其mRNA的表达有关。
(4)电项针可能通过下调脑缺血区粘附分子ICAM-1蛋白及其mRNA的表达而防治脑缺血再灌注炎性损伤,发挥神经元保护作用。
(5)通过对照研究发现电项针治疗在以上几方面均优于电体针组,说明电项针疗法是治疗脑缺血的有效方法。
As a familiar disease, Apoplexy has taken place frequently and the ratio of death and lameness are both very high. Moreover, the cerebral ischemia accounts for 75 percent of Apoplexy, it also has a tendency for increasing year after year . With the application of thrombolytic therapy , the reperfusion injury is paid to more and more attention . Studies showed that the cerebral ischemiareperfusion injury was closely associated with neuronal cell apoptosis, inflammatory reaction, overload of calcium , production of oxygen free radicals and toxicity of excitatory amino acid . So it is an urgent problem for us to prevent and cure the cerebral ischemia or to decrease the ratio of incident and lameness. This topic studies the cerebral protection of electro-nape acupuncture to ischemical reperfusion injury and Potion as well as its mechanism action.
Objective : To observe the expression of 1CAM-1 and that of the correlative gene of apoptosis in ischemic penumbra in rats after acute cerebral infarction and to explore the effect and the possible mechanism of electro-nape acupuncture therapy.
Methods : The model of focal cerebral ischemia was made by blocking the middle cerebral artery with monofilament in rats. The middle cerebral artery ocelussion rats were randomly divided into 4 groups: sham group (group A), model group (group B), electro-acupuncture group(group C) and electro-nape acupuncture group (group D). Male Wistar rats were divided randomly into four groups:sham group, model group, electro-nape acupuncture group and electro-acupuncture group group;each group was separated into 12h, 1d, 3d, 5d after rerperfusion as subgroup for investigation. HE staining and TUNEL were used to observe the changes of histomorphology. Took Immunohisto chemistry and In situ hybridization to detect the expression of Bcl-2、Fas-1( mRNA)、ICAM-1( mRNA), and also observe ultrastructure in brain tissue、nerve function Score.
Results: Electro-nape acupuncture has the obvious auxoaction on recuperation of neurologic impairment. The results showed electro-nape acupuncture possessed protective effect against cerebral ischemia -reperfusion injury. compared with the model group. Electron microscope results indicated that compared with group B, the neuron ultrastructure was mended in group C and in group D.It was more dominant in group D than that in group C. Electro-nape acupuncture can lessen neurologic impairment. Results pointed out, that compared with the model group, electro-acupuncture group and electro-nape acupuncture can increase the expression of Bcl-2 and restrain the expression of Fas-1(mRNA), ICAM-1(mRNA).
Conclusion: Electro-acupuncture group and electro-nape acupuncture can save the neurocytes in peri-infarct region and reinforce the blood supply so as to amend neural function. Electro-nape acupuncture can lessen neurologic impairment, repress apoptosis, promote Bcl-2 expression,heighten Fas-l(mRNA)expression and inhibit ICAM-1(mRNA) expression, which indicates that the action on repressing apoptosis is related to the regulation on Bcl-2, Fas-1(mRNA), ICAM-1(mRNA) expression.
目的:观察大鼠CIR后缺血半暗区ICAM-1和凋亡相关基因表达的变化规律及电项针对其的影响,探讨电项针治疗脑梗死可能的作用机制。
方法:采用栓线法制备局灶性脑缺血动物模型,将Wistar大鼠随机分为假手术组(A)、模型组(B)、电体针组(C)及电项针组(D),各组随机分成12h、1d、3d、5d四个时间段,对大鼠进行实验观察。(1)参照Zea-Longa的5级评分标准对其神经功能障碍进行评分;(2)通过HE染色观察病理形态学变化;(3)电镜观察脑组织超微结构的变化:(4)通过TUNEL法检测细胞凋亡;(5)应用免疫组织化学方法检测缺血半暗区Bcl-2、Fas-1、ICAM-1的蛋白表达;(6)应用原位杂交技术检测缺血半暗区Fas-1 mRNA和ICAM-1 mRNA的表达。
结果:
(1)模型组脑缺血再灌注后出现明显神经功能缺损,与假手术组比较差异显著(P＜0.01),证明脑缺血再灌注损伤实验模型成功;电体针组、电项针组与模型组比较差异显著(p＜0.01,p＜0.05);电项针组与电体针组比较亦有显著性差异(p＜0.05)。说明电项针对神经功能缺损的恢复有明显的促进作用。
(2)HE染色显示:与模型组比较电体针组和电项针组缺血半暗区神经细胞肿胀减轻,胶质细胞增生,神经元数量和新生毛细血管增多;电项针组较电体针组明显。
(3)电镜观察显示:与模型组比较电体针组和电项针组神经细胞超微结构的变化明显改善,电项针组优于电体针组。
(4)电体针组和电项针组均有很好地抑制细胞凋亡的作用,与模型组比较均有显著性差异(p＜0.05,p＜0.01);电体针组与电项针组3d和5d时比较有显著性差异(p＜0.05)。可见,电项针组抑制细胞凋亡作用最优。
(5)电体针组和电项针组与模型组比较,可明显提高Bcl-2蛋白表达水平,并在早期抑制Fas-1蛋白及其mRNA的表达、ICAM-1蛋白及其mRNA的表达(p＜0.05,p＜0.01)。同时间点电项针组与电体针组比较有显著性差异(p＜0.05)。
结论:
(1)电项针可有效促进脑缺血再灌注大鼠神经功能的恢复。
(2)电项针可促进缺血半暗带区胶质细胞和毛细血管增生,减轻神经元损伤,改善神经细胞的超微结构。
(3)电项针有较好地抑制细胞凋亡的作用,可促进Bcl-2蛋白表达,抑制Fas-1蛋白及其mRNA的表达,表明其抑制神经细胞凋亡的作用与调控Bcl-2蛋白和Fas-1蛋白及其mRNA的表达有关。
(4)电项针可能通过下调脑缺血区粘附分子ICAM-1蛋白及其mRNA的表达而防治脑缺血再灌注炎性损伤,发挥神经元保护作用。
(5)通过对照研究发现电项针治疗在以上几方面均优于电体针组,说明电项针疗法是治疗脑缺血的有效方法。
As a familiar disease, Apoplexy has taken place frequently and the ratio of death and lameness are both very high. Moreover, the cerebral ischemia accounts for 75 percent of Apoplexy, it also has a tendency for increasing year after year . With the application of thrombolytic therapy , the reperfusion injury is paid to more and more attention . Studies showed that the cerebral ischemiareperfusion injury was closely associated with neuronal cell apoptosis, inflammatory reaction, overload of calcium , production of oxygen free radicals and toxicity of excitatory amino acid . So it is an urgent problem for us to prevent and cure the cerebral ischemia or to decrease the ratio of incident and lameness. This topic studies the cerebral protection of electro-nape acupuncture to ischemical reperfusion injury and Potion as well as its mechanism action.
Objective : To observe the expression of 1CAM-1 and that of the correlative gene of apoptosis in ischemic penumbra in rats after acute cerebral infarction and to explore the effect and the possible mechanism of electro-nape acupuncture therapy.
Methods : The model of focal cerebral ischemia was made by blocking the middle cerebral artery with monofilament in rats. The middle cerebral artery ocelussion rats were randomly divided into 4 groups: sham group (group A), model group (group B), electro-acupuncture group(group C) and electro-nape acupuncture group (group D). Male Wistar rats were divided randomly into four groups:sham group, model group, electro-nape acupuncture group and electro-acupuncture group group;each group was separated into 12h, 1d, 3d, 5d after rerperfusion as subgroup for investigation. HE staining and TUNEL were used to observe the changes of histomorphology. Took Immunohisto chemistry and In situ hybridization to detect the expression of Bcl-2、Fas-1( mRNA)、ICAM-1( mRNA), and also observe ultrastructure in brain tissue、nerve function Score.
Results: Electro-nape acupuncture has the obvious auxoaction on recuperation of neurologic impairment. The results showed electro-nape acupuncture possessed protective effect against cerebral ischemia -reperfusion injury. compared with the model group. Electron microscope results indicated that compared with group B, the neuron ultrastructure was mended in group C and in group D.It was more dominant in group D than that in group C. Electro-nape acupuncture can lessen neurologic impairment. Results pointed out, that compared with the model group, electro-acupuncture group and electro-nape acupuncture can increase the expression of Bcl-2 and restrain the expression of Fas-1(mRNA), ICAM-1(mRNA).
Conclusion: Electro-acupuncture group and electro-nape acupuncture can save the neurocytes in peri-infarct region and reinforce the blood supply so as to amend neural function. Electro-nape acupuncture can lessen neurologic impairment, repress apoptosis, promote Bcl-2 expression,heighten Fas-l(mRNA)expression and inhibit ICAM-1(mRNA) expression, which indicates that the action on repressing apoptosis is related to the regulation on Bcl-2, Fas-1(mRNA), ICAM-1(mRNA) expression.
引文
1.张国瑾,赵增荣.国外脑血管疾病研究进展[M].北京:中国医药科技出版社.2000,177-197.
2.王永炎.中医内科学.上海科学技术出版社.1997,124
3.徐木林.中风之吉与今.辽宁中医杂志.1996,23(6):253
4.张树泉,王白玲.苗香辅治疗脑梗塞的经验.中医杂志.1998,38(1):24
5.段丽梅“治风先治血,血行风自灭”在中风病中的运用.河南中医.1998,18(4):229
6.雷燕.论瘀毒阻络石络病形成的病理基础.北京中医药大学学报.1999,(2):8-10
7.王永炎.中风病研究进展述评.中国中医急症.1995,4(2):51-54
8.车艳艳,向军,等.中药抗脑缺血再灌注损伤的实验研究概况.中国康复.2007,22(6):433
9.林春,黄扬,徐隽,等.蕲蛇酶对大鼠脑缺盘再灌注损伤的保护作用[J].中国药理学通报,2005,21(9):1100-1103.
10.张岫美,曹晓勃,魏欣冰,等.绞股蓝总皂苷对大鼠局灶性脑缺血再灌注损伤的抗氧化作用[J].山东医科大学学报.2002,40(1):7-9.
11.董少龙,闰珊珊,窦维华.水蛭注射液对大鼠脑缺血再灌注损伤神经细胞凋亡的影响[J].广西中医药.2006,29(1):48-50.
12.陈东风,赖真,等.针刺对脑缺血再灌注损伤神经细胞信号传导影响的研究进展.中国现代医生.2007,45(15):147
13.陈松清,王一萍,李怡新.颈部针刺治疗急性缺血性脑卒中95例[J].中国康复理论与实践.1997,3(4):161.
14.Mark Silver.Acupuncture wins BMA approval[J].BMJ.2000,321:11.
15.刘伍立,欧阳建军,黄博辉.中医文献对中风病的阐述与述评.针灸临床杂志2006,22(10):5-8
16.徐佳,等.远近部位穴位对脑缺血大鼠脑组织Ca~(2+)、Na~+、K~+含量影响[J].上海针灸杂志.2001,(5):36
17.于致顺等.《黑龙江针灸论文汇编》1980,1:8
18.贺静松等,汤颂延头针疗法《论文汇编》
19.石宪,等.头部“菱形区”治疗中风偏瘫的临床研究.针灸学报.1991,7(3):35
20.东贵荣,等头穴透刺治疗偏瘫的研究近况.针灸学报.1992,8(1):54
21.孙尧其.头针导引治疗偏瘫660例.上海针灸杂志.2001,20(4):34
22.马松涛.头针治疗中风偏瘫的临床观察.河北中医.1993,15:28
23.靳瑞,赖新生,李艳慧,等.颞三针治疗中风后遗症的临床观察.中国针灸.1993(1):11
24.聂卉.头穴针刺捻转速度治疗中风偏瘫的研究.针灸学报.1992,8(1):29
25.李艾君.针刺时机对中风康复的影响.四川医学.2001,22(4):390
26.石学敏.针刺治疗中风病的临床研究上海针灸杂志 1992,4:4
27.章小平,胡小新.CT定位头部围针治疗中风偏瘫的疗效观察.中医药学刊.2004,22(10):1961-1962
28.王克健,等.独取阳明法治疗中风恢复期的临床研究.中国针灸.1996,16(15):15-17
29.黄晓洁,等.芒针透刺法治疗偏瘫140例疗效观察.针灸临床杂志.2000,(3)23-25
30.庞勇,李保良.不同穴位治疗缺血性中风的临床研究.中国针灸.2000,20(2):69-72
31.朱红影,醒脑开窍法与头针治疗中风偏瘫的临床观察.上海针灸杂志.2000,19(1):16
32.马岩番.手十二井穴刺络放血对实验性脑缺血大鼠缺血区脑组织氧分压影响的动态观察.上海针灸杂志.2000(1):40
33.马岩番.手十二井穴刺络放血对实验性脑缺血大鼠缺血区脑组织钙调素活性影响的实验观察.针刺研究.1999,24(2):105-107
34.王秋云.按期分经电针法治疗中风后偏瘫疗效观察.中国针灸.2006,26(1):33-35
35.张汉粱.不同取穴方法治疗缺血性中风后偏瘫的临床研究.中国针灸.2002,22(11):735-738
36.雷龙鸣,庞军,陈家兴等.不同针刺方案对偏瘫早期康复作用的临床观察.中国针灸.2005,25(4):233-236
37.张鹤宾,林映欣.电针对脑卒中后上肢痉挛模式的影响[J].中国针灸.2003,23(5):310
38.喻澜,黄晓琳,王伟.电针对急性脑梗死患者运动功能和ADL的影响[J].中国康复.2005,20(6):337-338
39.黄凤兰.电针治疗脑血管意外后遗症262例.针灸临床杂志.2000(2):35
40.司全明.电针治疗急性脑梗塞患者临床疗效观察.中国针灸.1999,19(3):137
41.胡智慧,顾晓园 电针缓解中风偏瘫痉挛的疗效观察.中国针灸.1999,19(4):205
42.刘克英,泰培森,刘秀梅,等.电针阳明经穴治疗缺血性脑卒中及经颅彩色多普勒超声观察.中国针灸.2000,20(12):735-737.
43.吴勇.电针刺激神经干治疗中风偏瘫痉挛期疗效观察.湖北中医杂志.2005,27(12):38-39
44.毓英.眼针治疗腔隙性脑梗塞75例临床观察.山西中医.1995,(6):35
45.黄晓洁.眼针治疗中风后遗痘90例疗效观察.中国针灸.1996(5):7
46.赵欲晓.舌针为主治疗中风后遗症58例疗效观察.针灸临床杂志.2000,16(4):36
47.陈艳环.舌三针治疗中风后遗症语障碍12例临床体会.针灸临床杂志.1996,12(10):12
48.王海东,赵俊喜,李永升.督脉穴位注射疗法治疗中风187例.中医研究.2006,19(6):56-57
49.王玉华,王保才,宫丽丽,多穴齐灸与针刺治疗脑卒中后遗症的疗效比较.中国临床康复.2006,10(31):155-156
50.杨颖.腹针治疗中风后遗症的临床观察.辽宁中医学院学报.2006,8(3):101-102
51.宋京英,翟素萍,申玮红.早期应用通腑针刺法治疗脑卒中疗效观察.中国针灸.2002,22(6):369-370
52.Meyer R,Weissert R,Diem R,Storch MK,de-Graaf KL,Kramer B,Bahr M,Acute neuronal apoptosis in a rat model of multiple sclerosis.J Neurosci.2001,21(16):6214-6220
53.叶诸榕.脑缺血的细胞死亡:坏死或凋亡[J].中华神经科杂志.1998,31(5):301-304.
54.Arends M.J,Wyllie A.H.Apoptosis:mechanisms and roles in pathology[J].Int Rev Exp Pathol.1991,32:223-254.
55.Kruman I,Guo Q,Mattson MP.Calcium and reactive oxygen species mediate staturo sporrine-induced mitochondrial function and apoptosis inPC12 cells[J].Journal of Neuro science.2000,51(3):293-308.
56.刘煜敏,徐仁,黄怀钧,等.兴奋性氨基酸、氧自由基与缺血再灌注损伤关系的实验观察[J].卒中与神经疾病.2001,7(2):98-100.
57.Memezawa H,Smith ML,Siesjo BK.Penumbral tissue salvaged by reperfusion following middle cerebral artery occlusion in rats[J].Stroke.1992,23:552-559
58.Kristal BS,Dubinsky JM Mitochondrial permeability tromsition in the central nervous ystem:inductionby calicium cycling-dependent and inplendentpathways[J].JNeurochem.1997,69(2):524-538
59.Lewen A Matz P,Chan PH.Free radical pathways in CNS injury[J].J Neurotrauma.2000,17(10):871-890.
60.李曼霞,董志.脑缺血再灌注时血脑屏障损伤的炎性机制研究进展.解放军药学学报,2007,23(1):60
61.姜招峰,周翔,杨翰仪.氧自由基对人鼠海马及皮层的损伤作用[J].中国老年学杂志.2002,22(3):158
62.张田,魏子峰,等.中医药对脑缺血再灌注脑损伤保护作用研究的新进展.华北煤炭医学院学报.2007,9(2):190
63.Hignchi Y.increase in NO in the hypoxic-ischemic neonatalrat brain and supperession by 7-N1 and aminoguanidine[J].Eue J Phartnacol.1998,342(1):47-49.
64.Kumura M,Inao S,Takayasu M,et al.Time course of expression of the three nitric oxide synthase isoforms after transient middle cerebral artery occlusion in rats[J].Neurol Med Chir(Tokyo)2001,41(2):63-72.
65.张会欣,张建新一氧化氮在脑缺血脑损伤中作用的实验研究进展.中国药理学通报.2004,20(10):1094-1097
66.Peeters Scholte C,Koster J,Veldhuis W,et al.Neuroprotection by selective nitric oxide synthase inhibition at 24 hours after perinatal hypoxia ischemia.Stroke.2002,33:2304-2310.
67.李琳,张志强.脑缺血再灌注损伤中细胞凋亡的研究进展.中华物理学与康复杂志.2005,27(1):60-62
68.陈敏,李长青.钙与脑缺血再灌注后神经细胞凋亡.脑与神经疾病杂志.2003,11(4):253-255.
69.Dcnton RM,Mccormack JG.Ca2+transport by mammalian mitochondria and its role in hormone action[J].Am J Physiol.1997,249(6):543-554.
70.Lewen A,Matz P,Chan PH.Free radical pathways in CNS injury.Neurotrauma. 2000,17(10):87-90
71.Kristian T,Siesjo BK.Calcium-related damage in ischemia[J].Life Sci.1998,59(6):357-367.
72.Lopachin RM,Gaughan CL,Lehning EJ,Weber ML,Taylor CP.Effects ofion channel blockade on the distribution of Na,K,Ca and other elements inoxygen-glucose deprived CA 1 hippocampal neurons.Neurosci.2001,103(4):971-983
73.Fjimura M,Morita2Fujimura Y,Noshita N,et al.The cytosolic antioxidant copper zinc superoxide dismutase prevents the early release of mitochondrialcytochrome C in ischemic brain after transient focal cerebral ischcmia in micc.JNcurosci.2000,20:2817-2824.
74.Nishizawa Y Glutamate release and neuronal damage in ischemia[J].Life Sci.2001,69;369-381
75.Kiyoshi Katooka,Hisao Yanase.Mild hypothermia-a revived countermeasure against ischemic neuronal damages[J].Neuroscience research.1998,32:103-117
76.Nakayama R,Yano T,Ushijima K,et al.Effects of dantrolene on extracellular glutamate concentration and neuronal death in the rat hippocampal CA1 region subjected to transient ischemia[J].Anesthesiclogy,2002,96(3):705-710
77.Nishizawa Y.Glutamate release and neuronal damage in ischemia[J].Life Sci.2001,69(3):369-381.
78.Dohmen C,Kumura E,Rosner G,et al.Extracellular correlates of glutamatetoxicity in short-term ischemia and repe rfusion:a direct in vivo comparison between white and matter.Brain Res.2005,1037(1-2):43.
79.Borner C,Monney L.Apoptosis without caspases:an inefficient molecular guillotine? Cell Death Differ,1999,6(6):497-507
80.Nicholson DW,Tbornberry NA.Caspases:killer proteases.Trends Biochem Sci.1997;22(8):299-306
81.Li Y,Chopp M,Powers C,ct al.Apoptosis and protein expression after focal cerebral ischemia reperfussion in rats[J].Brain Res.1997,765(2):361.
82.Ferrer I,Friguls B,Dalfo E,Justicia C,Planas AM.Caspase-dependent and caspase-independent signalling of apoptosis in the penumbra following middlecerebral artery occlusion in the adult rat.Neuropathol Appl-Neurobiol.2003,29(5):472-481
83.Lee SH,Kim M,Kim YJ,Kim YA,Chi JC}Roh JK,Yoon BW.Ischemic intensity influences the distribution of delayed infarction and apoptotic cell deathfollowing transient focal cerebral ischemia in rats.Brain Res.2002,956(1):14-23
84.陈红兵,王鲁民,郭云良.Caspase与脑缺血研究进展.中国全科医学.2003,6(10):874-875
85.Fjimura M,morita-Fujimura Y,Noshita N,et al.The cytosolic antioxdant copper/zinc- superoxcidc dismutasc prevents the carly release of mitochondrial cytochrome C in ischemic brain after transi ent focal cerebral ischemia in mice [J].J Neurosci.2000,20(8):2817-2824
85.Muzio M,Chinnaiyan AM,Kischkei FC,et al.FLICE,a novel FADD-homologous ICE/CED-3 like protease,is recruited to the CD 95(Fas/Apo-l)death-inducing signaling complex[J].Cell.1996,85(6):817-827
87.ayashi Y,Tikhara I,Yagi T,et al.Immunohistochemical investing ation of caspase-1 and ffectof caspase-1 inhibitor indelayed neuronat death after transient cerebral ischemia[J].Brain Res.2001,893(1-2):113-120
88.Endres M,Namura S,Shimizu-Sasamata M.Attenuation of delayed neuronal death after mild focal ischemia in mice by inhibition of the caspase family.Cereb Blood Flow Metab.1998,18(3):238-247
89.Adams JM,Cory S.life or death decision by the Bcl-2protein family[J].Trends Biochem Sci.2001,26:61-66.
90.李祥,杨华.Bcl-2与缺血性脑血管疾病关系的研究进展[J].国外医学老年医学分册.2001,22(2):62-65.
91.朱铁梁,郝素云,段姚尧.脑缺血后Bcl-2家族、即早基因的表达与细胞凋亡.武警医学院学报.2006,15(2):166
92.Zhong L,Sarafian T,Kane D,et al.Bcl-2 inhibits death of central neural cells induced by multiple agents[J].Prol Natl Acad Sci.1993,90:4533-4537
93.Zhu Y,Prehn J,Culmsee C,et al.The β2-adrenoceptor agonist denbuterol modulates Bcl-2,Bcl-xl and Bax protein expression following transient forebrain ischemia[J].Neurosci.1999,90(4):1255-1263
94.Cai J,Jones DP.Superoxide in apoptosis.Mitochondrial generation by cytochrome closs[J].J Biol Chem 1998,273(19):11401-11404.
95.Kruman I,Bruce-Kelle AJ,Bredesen D,et al Evidence that 4 hydroxynonenal mediates oxidativestress-induced neuronal apoptosis[J].Neurosci.1997,17(13):5089-5100.
96.Shimazaki K,Ishida A,Kawai N.Increase in bcl-2 oncoprotein and the tolerance to ischemia-induced neuronal death in the gerbil hipoocampus.Neurosci Res.1994;20(1):95-99
97.KirinoT.Ischem is tolerance[J].J Ceteb Blood Flow Metab.2002(22):1283
98.Ferrer I,Lpez R,Blanco R,et al.Bcl-2,bax,and bcl-x exprssion in the CA1 area of the hippocampus following transient forebrain ischemia in the adult gerbil [J].Exp Brain Res.1998,121(2):167-173.
99.Martinon JC,Dubois-Dauphin M,Staple JK,et al.Overexpression of Bcl-2intransgene mice protects neurons from naturally occurring cell death and experimental ischemia[J].Neuron,1994,13:1017-1030.
100.刘丹,韩威,张迪.不同时间针刺对局灶性脑缺血再灌注大鼠BCL-2、Bax蛋白表达的影响.中华中医药学刊.2007,25(3):622
101.Choi JM,Shin Hk,Kim KY,Lee JH,Hong KW.Neuroprotective effect of cilostazol against focal cerebral ischemia via antiapoptotic action in rats.J pharmacol Exp Ther.2002,300(3):787-793
102.曲友直,赵燕玲,秦怀洲,等.川芎嗪对脑缺血再灌注后神经细胞凋亡及其相关蛋白Fas-L表达的影响.中国中医急症.2007,16(5):581
103.卫立辛,钱其军.Fas介导凋亡的免疫学研究进展[J]。国外医学·生理病学与临床分册.1997,17(4):281
104.narl M,Hug H,Nagata S.Involvement of an ICE-like protcasc in Fas-,nediated apoptosis[J].Nature.1995,375(6526):78-81
105.Itoh N,Nagata s.A novel protein domain required for apoptosis[J].J Biol chem.1993,268(15):10932-10937
106.Ahbas AK.Eliominating dangerous lymplmoytes[J].Cell.1996,84:655-657
107.Matsuyama T,Hata R,Yamamoto Y,et al.Localization of Fas antigen mRNA induced in postischemic murine forebrain by in situ hybridization.Brain Res Mol Brain Res,1995,34(1):166-172
108.Frigerio S,Silei V,Ciusani E,et al.Madulation of Fas-ligand(Fas-L)on human mincrolial cellsia in vitro study[J].J Neurommunol Suppl.2000,105(2):109-114
109.Martin-Villalba A,Hahne M,Kleber S,et al.Therapeutic neutralization of CD95-ligand and TNF attenuates brain damage in stroke.Cell Death Differ.2001,8(7):679-686
110.郝久宽,于维东,白抚生,等.大鼠局灶性脑缺血再灌注后Fas表达与细胞凋亡关系的研究[J].脑与神经疾病杂志.2001,9(5):272-273
111.兰希发,姚文秀,郭阳,脑缺血再灌注后神经细胞凋亡的机制[J].中国临床康复.2003,7(19):2726-2727
112.孔立红,任康,周华,等.电针对脑缺血再灌注大鼠海马Fas蛋白表达的影响.中国中医急症.2006,15(11):1252
113.林海,刘煜,李柱一,等.局灶性脑缺血再灌注后白细胞介素-6、肿瘤坏死因子-α表达及川芎嗪对其表达的影响[J].中国临床康复.2002,6(7):968-969
114.黄建梅,唐一鹏,洪庆涛.抗呆1号对体外模拟脑缺血再灌注损伤海马神经元凋亡调控基因表达的影响[J].北京中医药大学学报.2002,25(1):38
115.Neumann-Haefelin T,Wiessner C,Vogel P,et al.Differential expression of the immediate early genes c-Fos,c-jun,junB,and NGFI-B in the rat brain following transient forebrain ischemia[J].Cereb Blood Flow Metab.1994,14(2):206-216 33.
116.Lee MC,Rho JL,Kim MK,et al.c-JUN expression and apoptotic cell death in kainate-induced temporallobe epilepsy[J].J Korean Med Sci.2001,16(5):649-656
117.Kokubo Y,Matson GB,Liu J et al.Correlation between changes in apparent diffusion coefficient and induction of heat shock protein,cell-specific injury marker expression,and protein synthesis on diffusion-weighted magnetic resonance images after temporary focal cerebral ischemia in rats[J].J Neurosurg.2002,96(6):1084-1093
118.Hata R,Maeda K,Hermann D,etal.Dynamics of regional brain metabolism and gene expression after middle cerebral artery occlusioninmice[J].J Cereb Blood Flow Metab.2000.20(2):306-315.
119.诸榕.脑缺血的细胞死亡:坏死或凋亡.中华神经科杂志[J].1998.31(5):301-304.
120.Garcia JH,Ye ZR,kiu KE.Delayed neuronal death in experimental ischemicstroke[M].In:Touetal.eds:Maturation phenomenon in cerebral ischemia.Berlin:Springer-Verlag.2002,25(6):267-273.
121.HarrisonW,Beyaert R,Declercq W,Vandenabeele P More than one way to ie:apoptosis and necrosis and reactive oxygen damage[J].Onco gene.1999,18(54):7719-7730
122.Morganti-Kossmann MC,Ranoan M,Stahcl PE,et al.Inflammatory response in axute rtaumatic brain injury,a double-edged sword[J].Currp Opin Crit Car.2002,8(2):101-105
123.Rothwell N.Interleukin-landneuronal injury:mechanis-ms,modification,and the rapeutic potential[J].Brain Behavlmmun.2003,17(3)147-152
124.Emerich DF,Dean RL3rd Bartus RT,etal The role of leukocytes following cerebral ischem is pathogenic variable or bystander reaction to emerging infarcts[J].Exp Neurol 2002,168-173
125.Liao SL,Chen W Y,Raung SL,Kuo JS,Chen CJ.Association of immune res-ponse and ischemic brain infarction in rat.Neuroreport.2001,12(9):1943-1947
126.张田,魏子峰.中医药对脑缺血再灌注脑损伤保护作用研究的新进展.华北煤炭医学院学报.2007,9(2):190
127.徐皓亮,冯亦璞.黏附分子与脑缺血后炎症[J].生理科学进展.2000,31(3):257
128.田金洲.血管性痴呆.北京:人民卫生出版社.2003:253
129.张莉莉,王景周,周华东,等.肿瘤坏死因子和细胞黏附分子在脑缺血再灌注损伤中的表达规律[J].中国临床康复.2002,6(19):2968-2969
130.陈邦国,毛庆菊,孔立红.电针对局灶性脑缺血再灌注大鼠缺血脑区细胞黏附分子表达的影响.湖北中医学院学报.2006,8(4):3
131.Frijus CJ,appelle LJ.Inflammatory cell adhesion molecules ischemic cerebrovascular disease[J].Stroke 2002,33(8):2115-2122
132.高伟,梁日欣,肖水庆,等.川芎内酯A预处理对大鼠离体心脏缺血再灌注所致血管内皮细胞损伤的保护作用[J].中国中药.2005,30(18):1448-1552.
133.Preckl B,ScJilack W,Comfcrc T.Effect of dantrolene in an in vivo and in vitro,model of myocardial reperfusion injury[J].Actar- Anaesthesiol-Scand.2000,44(2):194-198.
134.何志文,欧阳嶷,张强,等.老年急性血管病患细胞因子及其受体的研究.中华老年心脑血管病杂志.2001,3(5):322
135.向红兵,晋光荣,吴颢听,等.小鼠多发性脑梗死后脑组织内皮细胞ICAM-1表达的变化.东南大学学报(医学版).2004,23(5):295
136.Del Zoppo GJ.Microvascular responses to cerebral ischem is/in flamma tion Ann NYA Cad Sci.1997,823:132-147
137.Stanim irovic D,Shapiro A,Wang J.et al.The induction of ICAM-I in human cerebrom icrovascular endothelial cells(HCEC)by ischem ialike conditions promotes enhanced neutrophil/HCEC adhesion J Neuro immuno.1997,76(12):193-205
138.杨倩,吴家幂,陈绍兰.细胞间黏附分与脑缺血再灌注脑损伤.皖南医学院学报.2002,21(1):71-73
139.王伟,王雪松,阮旭中.海风藤新术脂素成分对缺血鼠脑细胞间黏附分了-1及其mRNA表达的影响.中华物理医学与康复杂志.2002,24(3):133-136
140.Brain SD,Williams TJ,Tippins JR,et al.Calcitonin gene-related peptide is apotent vasodiator.Nature.1985,313:54
141.Zhang KA.Oxidant stress regulation of ICAM-1 gene expression:differential activation and binding of the transcription factors AP-1.Int J Mol Med.1999,4(3):223-230.
142.SorianoA,Morgan BP,Tikkanen I,et al.Time course of complement activation and inhibitor expression after ischemic injury of rat myocardium[J].Am J Pathol.1999,144(2):1357-1358.
143.Vcmuganti R,Denlpscy R.IBcnvcn KK.Inhibition of intercellular aclhcsion molecule-1 Protein expression by antisense oligonuclectides is neurop rotective after transient middle cerebral artery occlusion in rat[J].Stroke 2004, 35:179-184.
144.BertiR,W illiams A.IMoffett JR,etal QQuantitative real-time RT-PCR analysis of inflammatory gone expression assocl-ated with ischem io-reperfusion brain injury[J],Cereb Blood Flwv Metab.2002,22:1068-1079.
145.Wang ZQ,ChenXC,YangGY,etal Inflammatory reaction after focal cerebral ischemia in mouse[J],Chin Med J Eng.2004,1(17):586-591.
146.Vemuganti R,Dempsey RJ,Bowen KK,et al.Inhibition of intercellular adhesion molecule-lprote in expression by antisense oligonucleotides is neuroprotective after transientmiddle cerebralartery occlusion in rat.Stroke.2004,35:179-184.
147.Berti R,Williams AJ,Moffett JR,et al.Quantitativereal-time RTPCR analysis of inflammatory gone expression associated with ischemia-reperfusion brain injury.)Cereb Blood Flow Metab.2002,22:1068-1079.
148.Love S,Barber R.Expression of P-selectin and intercellular adhesion molecule-1in humanbrain after focal infarction or cardiac arrest.Neuropathol Appl Neurobiol.2001,27:465-473.
149.孙丽莎,杨露春,程佩兰.达纳康对鼠脑缺血-再灌注后ICAM-1的影响.天津医药.2002,30(3):161-163
150.夏斌,邵福源,赵忠新,等.急性缺血性脑血管病患者血清可溶性黏附分子的变化.临床研究.2002,25(5):308-309
151.宋水江,郑晓红,闻树群,等.细胞间黏附分子1和碱性成纤维细胞生长因子在脑梗死中的作用.中华医学杂志.2002,82(21):1447-1449.
152.Konemoto.Y,Nakase H,Akita N,Sakaki J.Efects of anti- intercellular adhesion molecule-1 antibody on repeifusion injury ineluded by late repeifusion in the rat middle cerebral artery ocelusion models[J].Neurosygery.2002,51:1034-42
153.Bruce A,Boling W,Kindy MS,etal.Altered neuronal and microglial responses to excitotoxic and ischemic brain injury in mice lacking TNF receptors[J].Nat Med.1996,2(7):788-794.
154.吴玉泉,霍正禄.细胞粘附分子与脑梗死关系的研究进展.医学综述.2002,8(10):570-572
155.孙丽莎,杨露春,程佩兰.达纳康对鼠脑缺血-再灌注后ICAM-1的影响,天津医 药.2002,30(3):161-163
156.徐丽荣,马世彬,李澎涛,等.清开灵有效组份对局灶性脑缺血大鼠脑组织细胞间黏附分子表达的影响.中草药.2004,35(6):666-669
157.孙国兵,赵薛旭,李作汉,等.脑缺血再灌注大鼠细胞间黏附分子-1、血管细胞间黏附分子-1的表达及β-七叶皂甙钠的干预作用.临床神经病学杂志.2005,18(2):120-123
158.扈友庄,杨彬,刘东彪.葛根素对急性脑梗死患者血清sICAM-1,TNF-α水平的影响.中国医师杂志.2005,7(8):1135-1136
159.工亚贤,沈小苹,李明琦,等.刺五加注射液对大鼠局灶性脑缺血再灌注模型TNF-α和ICAM-1表达的影响.哈尔摈医科大学学报.2007,41(6):561
160.孔立,尤可,江涛,等.调气息风法对脑缺血大鼠ICAM-11L-1β及TNF-α表达的影响中国中医急症.2007,16(9):1098.
161.柳洪胜,张允岭,刘雪梅,等.清脑宣窍滴丸对大鼠局灶脑缺血再灌注炎症因子含量的影响,中华中医药杂志.2007,22(5):328
152.王玉升.电针对急性局灶性脑缺血模型大鼠的影响.中国针灸.1996,(9):34
153.司全明.电针对脑缺血大鼠体感诱发电位及脑便塞体积的影响.针刺研究.1998,(1):36
164.张秀花,孙世晓,徐博佳.井穴点刺出血对局灶性脑缺血大鼠脑梗塞体积影响的基础研究.针灸临床杂志.2004,20(12):47-48
165.周飞,李福风,程介士,等.电针对急性脑缺血大鼠局部脑血流和脑梗死体积的影响.上海针灸杂志.2003,22(5):3-6
166.裴海涛,郭壮丽.头穴针刺局灶性再灌注大鼠脑内TGF-β mRNA表达的调节.中国针灸.2003,23(12):739-741
167.张振强,刘丽娟,李建会.电针干预脑缺血再灌注大鼠梗死体积及脑内白细胞介素1β蛋白表达的变化,中国临床康复.2006,10(43):153-155
168.闫庆军.针刺人中对急性脑缺血大鼠海马rCBF神经元超微结构的影响.湖北中医学院学报.1999,1(1):28-30
169.许能贵,沈德凯,周逸平,等.电针对局灶性脑缺血大鼠发层体感诱发电位和细胞超微结构的影响.中医杂志.2001,42(6):342-346 30.
170.刘波,唐强,李静.针刺治疗缺血性脑损伤的实验研究.中国康复理论与实 践.2005,11(7):514-515
171.易玮,许能贵,汪帼斌,等.电针对局灶性脑缺血大鼠突触可塑性促进作用的实验研究.中国中西医结合杂志.2006,26(8):710-714
172.Kontos HA.Oxygen radicals in celebral ischemia:the 2001 Willis lecture[J].Stroke.2001,32:2712-2716
173.周国平,邓常青.手十二井穴放血抗兔实验性脑缺血机理的研究.针刺研究.1999,24(2):101
174.郑谅,周莹,李艳慧,等.针刺对中风过氧化脂质和抗氧化酶的影响.新中医.2001,33(6):42
175.许能贵,易玮,马勤耘.电针对大鼠局灶性脑缺血后神经元损伤保护作用的研究中国针灸.2000,20(4):237-240
176.陆任云,徐斌等.针刺对缺血再灌注脑组织形态结构和酶活性影响的实验研究.针刺研究.2002,27(1):5-9.
1177.辛随成,郭书文,王国华,针药结合对实验性脑缺血脑细胞凋亡及相关因素的影响.中国中医药信息杂志.2004,11(3):218-220
178.徐坚,陶陶,何燕,等.天麻及电针对大鼠脑缺血再灌注损伤的保护作用.中国脑血管病杂志.2004,1(5):221-224
179.许燕,陆任云,徐斌,等.针刺对脑缺血再灌注大鼠脑组织NOS及NO的影响[J].镇江医学院学报.1999,9(4):513-514
180.施静,张静,刘晓春,等.电针对局灶性脑缺血再灌注大鼠大脑皮层一氧化氮的影响[J].针刺研究.1999,24(4):268-273
181.张京钟,施静,刘晓春,等.电针对大鼠局灶性脑缺血后BAX,BCl-2表达的影响.中国组织化学和细胞化学杂志.2001,10(1):27
182.Jin GL,Su J.Ding SQ,Zhou DF.Theeffect of electroacupuncture on behavior and brain'smonoamine neurotransmitters of rat[J].Zhongguo Xingwei Yixue(Chin Behav Med Sci.2000,9(3):164-166
183.田菲.电针对缺血再灌注大鼠脑组织NO及自由基的影响[J].中国中医急症.1999,8(1):33
184.董裕.针刺对暂时性脑缺血脑内一氧化氮台成酶及神经细胞死亡的影响[J].钳刺研究.2000(1):8-13
185.许燕,等.针刺对脑缺血再灌注大鼠脑组织NOS及NO的影响.[J]镇江医学院学报.1993,9(4):513-514
186.郭义,胡利民,张严峻,等.手十二井穴刺络放血对实验性脑缺血大鼠缺血区细胞外液钙浓度影响的动态观察.针灸临床杂志.1999,15(6):48
187.马惠芳,孙华,任秀君,等.电针水沟与井穴对全脑缺血大鼠脑组织钙调素活性影响的对比研究[J].针灸研究.2002,27(2):102-104
188.许能贵,易玮,赖新生,等.电针对局造脑缺血大鼠脑细胞内Ca~(2+)含量的影响.中国中西医结合杂志,2002,22(4):295
189.金智秀,郝晋东,等.不同时间窗电针对急性局灶性脑缺血模型大鼠脑组织活性钙调素含量影响的研究.针刺研究.2003,28(3):178-181
190.金智秀,图娅,洪银珠,等.头针、体针和针药结合治疗对全脑缺血大鼠钙调素活性即刻影响的比较研究.针刺研究.2004,29(1):18-20 48.
191.霍则军,任秀君,刘青云.针刺不同穴组对脑缺血再灌注大鼠保护作用研究,山西中医.2004,20(1):47-48
192.姚凯,郭义,胡利民,等.针刺对大鼠脑缺血超早期脑细胞内外游离钙离子浓度的影响.中国中西医结合急救杂志.2005,12(5):303-305
193.施静,等.电针对脑缺血性神经元凋亡的影响[J].中国组织化学与细胞化学杂.1998,7:391
194.许能贵,马勤耘,侯思伟.电针对局灶性脑缺血大鼠兴奋氨基酸含量的影响.中国针灸.1997,17(7):431
195.郭景春,程介士.电针对脑缺血大鼠纹状体细胞外兴奋性氨基酸水平的影响.上海针灸杂志.2000,19(1):37-39.
196.邹军,张家维,赖新生等.电针对缺血性中风兴奋性氨基酸、c-fos及细胞凋亡影响.陕西中医.2002,23(8):760-761
197.角建瓴,赖新生.电针对局灶性脑缺血大鼠再灌流后四种氨基酸含量的影响.针刺研究.2003,28(3):170-173
198.甘平,郭景春,杨茹,等.针刺抗脑缺血损伤时脑内γ-氨基丁酸的作用.上海针灸杂志.2003,22(9):3-6
199.陈虎,蔡定芳.电针对局灶性脑缺血大鼠海马兴奋性氨基酸受体鲍影响.上海针灸杂志.2003,22(5):13-15
200.施静.针刺抗脑缺血性神经元凋亡作用与机制的研究.生理科学进展.1999,30(4):326-329
201.李成永,吴嘉容,沈国权,等推拿、针刺对急性脑缺血大鼠的脑保护作用.中医药信息.2003,20(6):44-45
202.李成永,李文金,苏维广,等.眼针对急性脑缺血大鼠的脑保护作用.辽宁中医杂志,2004,31(2):104-105
203.王红伟,徐华明.针刺对局灶性脑缺血再灌注大鼠脑细胞凋亡的影响.河南中医学院学报.2007,22(6):21
204.甘云波,黄光英,张明敏.针刺对大鼠局灶性脑缺血再灌注损伤后神经元凋亡的影响.山东医药.2007,47(15):24
205.唐伟,孙忠人,张力,等.钟刺预处理全脑缺血大鼠海马CA1区细胞凋亡和热休克蛋70mRNA的表达,中国临床康复.2005,9(37):90-92
206.唐伟,孙忠人,王卓尔,等.针刺预处理对大鼠脑缺血再灌注损伤的保护作用.中国中医药科技.2006,13(4):209-211
207.唐伟,孙忠人,王卓尔,等.针剌预处理对脑缺血再灌注损伤的保护作用.辽宁中医杂志.2006,33(5):627-628
208.孙忠人,唐伟,单丽莉,等.针刺诱导脑缺血耐受的实验研究.中国临床康复.2005,9(1):122-123
209.Jang MH,Shin MC,Lee TH,et al,Acupuncture sup-presses ischemia-induced I ncrease in c-Fos expression and apoptosis in the hippocampal CAI region in gerbils[J].Neu-rosci Lett.2003,347(1):5-8.
210.余晓慧.电针对局灶性脑缺血大鼠Caspase-3蛋白表达的影响.辽宁中医学院学报.2005,7(2):167
211.罗勇,董为伟.电针上调局灶性脑缺血再灌注时脑组织抗凋亡基因bel-2蛋白表达.中华物理医学与康复医学杂志.2000,22(1):53
212.邹军,赖新生,张家维,等.电针对缺血性中风细胞凋亡及Bel-2,P53的影响.四川中医.2002,(8):9
213.余晓慧.针刺对局灶性脑缺血大鼠脑细胞凋亡及Bcl-2蛋白表达的影响.针刺研究.2004,29(1):15-17
214.田代实,邓医宇,王光海,等.针刺预处理对大鼠局灶性脑缺血的抗细胞凋亡作用 第四军医大学学报.2004,25(1):27-29
215.孙忠人,张力,郑美华,等.针刺预处理对全脑缺血大鼠脑组织Bcl-2蛋白表达影响的实验研究.中医药学刊.2006,24(11):1976-1977
216.唐伟,王威,孙忠人,等.针刺预处理全脑缺血大鼠海马CA1区Bcl-2mRNA的表达.中国中医基础医学杂志.2007,13(2):156
217.孔立红,任婕,周华,等.电针对脑缺血再灌注大鼠海马Fas蛋白表达的影响.中国中医急症.2006,15(11):1252.
218.王利,张学勤,张勇,等.电针对大鼠脑局部缺血再灌注后c-fosmRNA表达的影响[J].中国针灸.2000 20(4):241-243
219.邹军,张家维,赖新生,等.电针对缺血性中风兴奋性氨基酸、c-fos及细胞凋亡影响.陕西中医.2002,23(8):760
226.王秀云,李积胜,郭义,等.手十二井穴刺络放血法对MCAO模型大鼠大脑皮层c-fos蛋白表达的影响.上海针灸杂志.2004,23(12):39-41152.
221.张春红,王舒,石学敏,等.醒脑开窍针法对脑梗死模型大鼠脑组织c-fos基因表达的影响.天津中医药,2004,21(3):210-213
222.孙忠人,唐伟,王威.针刺预处理诱导全脑缺血大鼠海马CA1区即刻早期基因c-fos mRNA及其蛋白的表达.中国临床康复,2006,10(19):125-127
223.王文远,赵建明,齐迎春.平衡针法对局灶性脑缺血大鼠血清TNF-α,stCAM-1的影响.临床军医杂志,2004,32(1):5-7
224.章薇,刘智,娄必丹,等.平衡针刺法对脑梗死患者IL-1和sICAM-1、sVCAM-1的影响[J].中国针灸.2005,25(3):214-216
225.刘玉珍,韩景献,姜文,等.醒脑开窍针法对脑缺血再灌注后细胞间黏附分子-1和P-选择素表达调节的实验研究.天津中医药.2005,22(6):462
226.苏红梅,朱广旗,欧阳冷星,等.针刺对大鼠脑缺血再灌注损伤后ICAM-1表达的影响.上海针灸杂志.2006,25(12):30
227.陈邦国,毛庆菊,孔立红.电针对局灶性脑缺血再灌注大鼠缺血脑区细胞黏附分子表达的影响.湖北中医学院学报.2006,8(4):3
228.孔立红,毛庆菊,陈邦国.电针对局灶性脑缺血再灌注大鼠细胞间黏附分子-1表达的影响.世界针灸杂志.2007,17(1):31
229.Zea-longa E,Weinstein PR,Carlson,et al.Reversible middle cerebral arteryocclusion without craniectomy in rat[J].Stroke 1989,20(1):84-91
230.Bederson JB.Et al.Rat middle cerebral artery occlusion using an intraluminal thread technique.Acta Neurchir.1991,109:126
231.高维滨.神经病针灸新疗法.人民卫生出版社.2002年12月第1版第1次印刷.
232.高碧霄,张跃全,等.十余年来百会研究概况.中医文献杂志.1997,(2):41-43
233.苗华,崔可密,卢岩,等.大鼠脑缺血模型的复制方法及评价.山东医药.2001,20(3):23-26.
234.杨元德.针灸治疗中风及后遗症近10年的文献概述.中国针灸,1992,(4):48-52.
235.关云谦,陈彪,周茗邹,等.尼龙线直径和硬度对大鼠线栓法脑缺血模型成功率的影响.中国医学科学院学报.2004,26(5):600.
236.Meyer R.Wcisscrt R.et al.Acute neuronal apoptosis in a rat modclof multiple selerosis J Neurosci,2001,21(16):6214-6220
237.Martin ZJ,Al-Abdulla NA,Bramrink.AM et al.Neurodegeneration in excitotoxicity,global cerebral ischemia,and target deprivation:aperspective on the contribution of apoptosis and necrosis.Brain Res Bul.1998,46:281-309.
238.霍则军,任秀君,刘青云.针剌不同穴组对脑缺血再灌注大鼠保护作用研究[J].西中医.2004,20(1):47
239.Martinou J-C Dubois IDauphin M,Staple JK,et al.Overexpression of bcl-2 in transgenic mice protects neurons from naturally occurring cell death and experimental ischemia Neuron.1994,13(4):1017-1013
240.Linnik MD Zahos P,Geschwind MD,et al.Expression of bcl-2 frema defect ive herpes sirr}lex virus-1 vector limits neuronal death in focalcerebral ischemia Stroke.1995,26(9):1670-1674
241.NagataS,GolsteinP.TheFas death factor.Science.1995,267(5203):1449-1456
242.刘勇,胡明均,何国厚.Fas/FasL的高表达在缺血性脑损伤中的意义.中国临床康复.2003,7(31):4184-4185
243.Martin-Villalba A,Herr I,Jeremias I,Hahne M,Brandt R,Vogel J,Schenkel J,Herdegen T,Debatin KM.CD95 ligand(Fast/Apo-1L)and tumor necrosisfactor-related apoptosis-inducing ligand mediate ischemia-induced apoptosis in neurons.J Neurosci.1999,19(10):3809-3817
244.Rosenbaum DM,Gupta G,D Amore J.Sihgh M,Weidenheim K,Zhang H,Kessler JA.Fas(CD95/Apo-1)plays a role in the pathophysiology of focal Cerebral ischemia.J Neurosci Res.2000,61(6):686-692
245.Zhang RL,et al.Brain Research.1995,682(1):182
246.Sobel RA,Mitebell ME,Fondren G.Intercellular adhesion molecule-1(ICAM-1)in cellular immune reasons in the human central nervous system.Am J Pathol.1991,136:1309-1316
247.Wang X,Feuerstein GZ.Induced expression of adhesion molecules following focalbrain ischemia.J Neurotrauma.1995,12(5):825-832
248.Clark WM,Lauten JD,Lessov N,et al.Time course of ICAM-1 expression andlcukocytc subsct infiltration in rat forcbrain ischcmia.Mol Chcm Neuropathol 1995,26(3):213-230
249.Zhang RL,Chopp M,Zaloga C,et al.The temporal profiles of ICAM-1 protein andmRNA expression after transient MCA occlusion in the rat.Brain Res.1995,682(1-2):182-188
250.Kitagawa K,et al.Deficiency of intercellular adhesion molecular-1attenuates microcirculatory disturbance and infarction size in focal ischemia.J Cereb Blood Flow Metab.1998.18(12):1336
251.张莉莉,等.大鼠脑缺血再灌注区细胞间粘附分子-1表达与白细胞浸润的实验研究.中国危重病急救医学.2000,12(5):264
252.沈德凯,等.电针对局灶性脑缺血大鼠神经元损伤保护作用的病理组织学研究。中国中医药科技.1998,5(5):269
2.王永炎.中医内科学.上海科学技术出版社.1997,124
3.徐木林.中风之吉与今.辽宁中医杂志.1996,23(6):253
4.张树泉,王白玲.苗香辅治疗脑梗塞的经验.中医杂志.1998,38(1):24
5.段丽梅“治风先治血,血行风自灭”在中风病中的运用.河南中医.1998,18(4):229
6.雷燕.论瘀毒阻络石络病形成的病理基础.北京中医药大学学报.1999,(2):8-10
7.王永炎.中风病研究进展述评.中国中医急症.1995,4(2):51-54
8.车艳艳,向军,等.中药抗脑缺血再灌注损伤的实验研究概况.中国康复.2007,22(6):433
9.林春,黄扬,徐隽,等.蕲蛇酶对大鼠脑缺盘再灌注损伤的保护作用[J].中国药理学通报,2005,21(9):1100-1103.
10.张岫美,曹晓勃,魏欣冰,等.绞股蓝总皂苷对大鼠局灶性脑缺血再灌注损伤的抗氧化作用[J].山东医科大学学报.2002,40(1):7-9.
11.董少龙,闰珊珊,窦维华.水蛭注射液对大鼠脑缺血再灌注损伤神经细胞凋亡的影响[J].广西中医药.2006,29(1):48-50.
12.陈东风,赖真,等.针刺对脑缺血再灌注损伤神经细胞信号传导影响的研究进展.中国现代医生.2007,45(15):147
13.陈松清,王一萍,李怡新.颈部针刺治疗急性缺血性脑卒中95例[J].中国康复理论与实践.1997,3(4):161.
14.Mark Silver.Acupuncture wins BMA approval[J].BMJ.2000,321:11.
15.刘伍立,欧阳建军,黄博辉.中医文献对中风病的阐述与述评.针灸临床杂志2006,22(10):5-8
16.徐佳,等.远近部位穴位对脑缺血大鼠脑组织Ca~(2+)、Na~+、K~+含量影响[J].上海针灸杂志.2001,(5):36
17.于致顺等.《黑龙江针灸论文汇编》1980,1:8
18.贺静松等,汤颂延头针疗法《论文汇编》
19.石宪,等.头部“菱形区”治疗中风偏瘫的临床研究.针灸学报.1991,7(3):35
20.东贵荣,等头穴透刺治疗偏瘫的研究近况.针灸学报.1992,8(1):54
21.孙尧其.头针导引治疗偏瘫660例.上海针灸杂志.2001,20(4):34
22.马松涛.头针治疗中风偏瘫的临床观察.河北中医.1993,15:28
23.靳瑞,赖新生,李艳慧,等.颞三针治疗中风后遗症的临床观察.中国针灸.1993(1):11
24.聂卉.头穴针刺捻转速度治疗中风偏瘫的研究.针灸学报.1992,8(1):29
25.李艾君.针刺时机对中风康复的影响.四川医学.2001,22(4):390
26.石学敏.针刺治疗中风病的临床研究上海针灸杂志 1992,4:4
27.章小平,胡小新.CT定位头部围针治疗中风偏瘫的疗效观察.中医药学刊.2004,22(10):1961-1962
28.王克健,等.独取阳明法治疗中风恢复期的临床研究.中国针灸.1996,16(15):15-17
29.黄晓洁,等.芒针透刺法治疗偏瘫140例疗效观察.针灸临床杂志.2000,(3)23-25
30.庞勇,李保良.不同穴位治疗缺血性中风的临床研究.中国针灸.2000,20(2):69-72
31.朱红影,醒脑开窍法与头针治疗中风偏瘫的临床观察.上海针灸杂志.2000,19(1):16
32.马岩番.手十二井穴刺络放血对实验性脑缺血大鼠缺血区脑组织氧分压影响的动态观察.上海针灸杂志.2000(1):40
33.马岩番.手十二井穴刺络放血对实验性脑缺血大鼠缺血区脑组织钙调素活性影响的实验观察.针刺研究.1999,24(2):105-107
34.王秋云.按期分经电针法治疗中风后偏瘫疗效观察.中国针灸.2006,26(1):33-35
35.张汉粱.不同取穴方法治疗缺血性中风后偏瘫的临床研究.中国针灸.2002,22(11):735-738
36.雷龙鸣,庞军,陈家兴等.不同针刺方案对偏瘫早期康复作用的临床观察.中国针灸.2005,25(4):233-236
37.张鹤宾,林映欣.电针对脑卒中后上肢痉挛模式的影响[J].中国针灸.2003,23(5):310
38.喻澜,黄晓琳,王伟.电针对急性脑梗死患者运动功能和ADL的影响[J].中国康复.2005,20(6):337-338
39.黄凤兰.电针治疗脑血管意外后遗症262例.针灸临床杂志.2000(2):35
40.司全明.电针治疗急性脑梗塞患者临床疗效观察.中国针灸.1999,19(3):137
41.胡智慧,顾晓园 电针缓解中风偏瘫痉挛的疗效观察.中国针灸.1999,19(4):205
42.刘克英,泰培森,刘秀梅,等.电针阳明经穴治疗缺血性脑卒中及经颅彩色多普勒超声观察.中国针灸.2000,20(12):735-737.
43.吴勇.电针刺激神经干治疗中风偏瘫痉挛期疗效观察.湖北中医杂志.2005,27(12):38-39
44.毓英.眼针治疗腔隙性脑梗塞75例临床观察.山西中医.1995,(6):35
45.黄晓洁.眼针治疗中风后遗痘90例疗效观察.中国针灸.1996(5):7
46.赵欲晓.舌针为主治疗中风后遗症58例疗效观察.针灸临床杂志.2000,16(4):36
47.陈艳环.舌三针治疗中风后遗症语障碍12例临床体会.针灸临床杂志.1996,12(10):12
48.王海东,赵俊喜,李永升.督脉穴位注射疗法治疗中风187例.中医研究.2006,19(6):56-57
49.王玉华,王保才,宫丽丽,多穴齐灸与针刺治疗脑卒中后遗症的疗效比较.中国临床康复.2006,10(31):155-156
50.杨颖.腹针治疗中风后遗症的临床观察.辽宁中医学院学报.2006,8(3):101-102
51.宋京英,翟素萍,申玮红.早期应用通腑针刺法治疗脑卒中疗效观察.中国针灸.2002,22(6):369-370
52.Meyer R,Weissert R,Diem R,Storch MK,de-Graaf KL,Kramer B,Bahr M,Acute neuronal apoptosis in a rat model of multiple sclerosis.J Neurosci.2001,21(16):6214-6220
53.叶诸榕.脑缺血的细胞死亡:坏死或凋亡[J].中华神经科杂志.1998,31(5):301-304.
54.Arends M.J,Wyllie A.H.Apoptosis:mechanisms and roles in pathology[J].Int Rev Exp Pathol.1991,32:223-254.
55.Kruman I,Guo Q,Mattson MP.Calcium and reactive oxygen species mediate staturo sporrine-induced mitochondrial function and apoptosis inPC12 cells[J].Journal of Neuro science.2000,51(3):293-308.
56.刘煜敏,徐仁,黄怀钧,等.兴奋性氨基酸、氧自由基与缺血再灌注损伤关系的实验观察[J].卒中与神经疾病.2001,7(2):98-100.
57.Memezawa H,Smith ML,Siesjo BK.Penumbral tissue salvaged by reperfusion following middle cerebral artery occlusion in rats[J].Stroke.1992,23:552-559
58.Kristal BS,Dubinsky JM Mitochondrial permeability tromsition in the central nervous ystem:inductionby calicium cycling-dependent and inplendentpathways[J].JNeurochem.1997,69(2):524-538
59.Lewen A Matz P,Chan PH.Free radical pathways in CNS injury[J].J Neurotrauma.2000,17(10):871-890.
60.李曼霞,董志.脑缺血再灌注时血脑屏障损伤的炎性机制研究进展.解放军药学学报,2007,23(1):60
61.姜招峰,周翔,杨翰仪.氧自由基对人鼠海马及皮层的损伤作用[J].中国老年学杂志.2002,22(3):158
62.张田,魏子峰,等.中医药对脑缺血再灌注脑损伤保护作用研究的新进展.华北煤炭医学院学报.2007,9(2):190
63.Hignchi Y.increase in NO in the hypoxic-ischemic neonatalrat brain and supperession by 7-N1 and aminoguanidine[J].Eue J Phartnacol.1998,342(1):47-49.
64.Kumura M,Inao S,Takayasu M,et al.Time course of expression of the three nitric oxide synthase isoforms after transient middle cerebral artery occlusion in rats[J].Neurol Med Chir(Tokyo)2001,41(2):63-72.
65.张会欣,张建新一氧化氮在脑缺血脑损伤中作用的实验研究进展.中国药理学通报.2004,20(10):1094-1097
66.Peeters Scholte C,Koster J,Veldhuis W,et al.Neuroprotection by selective nitric oxide synthase inhibition at 24 hours after perinatal hypoxia ischemia.Stroke.2002,33:2304-2310.
67.李琳,张志强.脑缺血再灌注损伤中细胞凋亡的研究进展.中华物理学与康复杂志.2005,27(1):60-62
68.陈敏,李长青.钙与脑缺血再灌注后神经细胞凋亡.脑与神经疾病杂志.2003,11(4):253-255.
69.Dcnton RM,Mccormack JG.Ca2+transport by mammalian mitochondria and its role in hormone action[J].Am J Physiol.1997,249(6):543-554.
70.Lewen A,Matz P,Chan PH.Free radical pathways in CNS injury.Neurotrauma. 2000,17(10):87-90
71.Kristian T,Siesjo BK.Calcium-related damage in ischemia[J].Life Sci.1998,59(6):357-367.
72.Lopachin RM,Gaughan CL,Lehning EJ,Weber ML,Taylor CP.Effects ofion channel blockade on the distribution of Na,K,Ca and other elements inoxygen-glucose deprived CA 1 hippocampal neurons.Neurosci.2001,103(4):971-983
73.Fjimura M,Morita2Fujimura Y,Noshita N,et al.The cytosolic antioxidant copper zinc superoxide dismutase prevents the early release of mitochondrialcytochrome C in ischemic brain after transient focal cerebral ischcmia in micc.JNcurosci.2000,20:2817-2824.
74.Nishizawa Y Glutamate release and neuronal damage in ischemia[J].Life Sci.2001,69;369-381
75.Kiyoshi Katooka,Hisao Yanase.Mild hypothermia-a revived countermeasure against ischemic neuronal damages[J].Neuroscience research.1998,32:103-117
76.Nakayama R,Yano T,Ushijima K,et al.Effects of dantrolene on extracellular glutamate concentration and neuronal death in the rat hippocampal CA1 region subjected to transient ischemia[J].Anesthesiclogy,2002,96(3):705-710
77.Nishizawa Y.Glutamate release and neuronal damage in ischemia[J].Life Sci.2001,69(3):369-381.
78.Dohmen C,Kumura E,Rosner G,et al.Extracellular correlates of glutamatetoxicity in short-term ischemia and repe rfusion:a direct in vivo comparison between white and matter.Brain Res.2005,1037(1-2):43.
79.Borner C,Monney L.Apoptosis without caspases:an inefficient molecular guillotine? Cell Death Differ,1999,6(6):497-507
80.Nicholson DW,Tbornberry NA.Caspases:killer proteases.Trends Biochem Sci.1997;22(8):299-306
81.Li Y,Chopp M,Powers C,ct al.Apoptosis and protein expression after focal cerebral ischemia reperfussion in rats[J].Brain Res.1997,765(2):361.
82.Ferrer I,Friguls B,Dalfo E,Justicia C,Planas AM.Caspase-dependent and caspase-independent signalling of apoptosis in the penumbra following middlecerebral artery occlusion in the adult rat.Neuropathol Appl-Neurobiol.2003,29(5):472-481
83.Lee SH,Kim M,Kim YJ,Kim YA,Chi JC}Roh JK,Yoon BW.Ischemic intensity influences the distribution of delayed infarction and apoptotic cell deathfollowing transient focal cerebral ischemia in rats.Brain Res.2002,956(1):14-23
84.陈红兵,王鲁民,郭云良.Caspase与脑缺血研究进展.中国全科医学.2003,6(10):874-875
85.Fjimura M,morita-Fujimura Y,Noshita N,et al.The cytosolic antioxdant copper/zinc- superoxcidc dismutasc prevents the carly release of mitochondrial cytochrome C in ischemic brain after transi ent focal cerebral ischemia in mice [J].J Neurosci.2000,20(8):2817-2824
85.Muzio M,Chinnaiyan AM,Kischkei FC,et al.FLICE,a novel FADD-homologous ICE/CED-3 like protease,is recruited to the CD 95(Fas/Apo-l)death-inducing signaling complex[J].Cell.1996,85(6):817-827
87.ayashi Y,Tikhara I,Yagi T,et al.Immunohistochemical investing ation of caspase-1 and ffectof caspase-1 inhibitor indelayed neuronat death after transient cerebral ischemia[J].Brain Res.2001,893(1-2):113-120
88.Endres M,Namura S,Shimizu-Sasamata M.Attenuation of delayed neuronal death after mild focal ischemia in mice by inhibition of the caspase family.Cereb Blood Flow Metab.1998,18(3):238-247
89.Adams JM,Cory S.life or death decision by the Bcl-2protein family[J].Trends Biochem Sci.2001,26:61-66.
90.李祥,杨华.Bcl-2与缺血性脑血管疾病关系的研究进展[J].国外医学老年医学分册.2001,22(2):62-65.
91.朱铁梁,郝素云,段姚尧.脑缺血后Bcl-2家族、即早基因的表达与细胞凋亡.武警医学院学报.2006,15(2):166
92.Zhong L,Sarafian T,Kane D,et al.Bcl-2 inhibits death of central neural cells induced by multiple agents[J].Prol Natl Acad Sci.1993,90:4533-4537
93.Zhu Y,Prehn J,Culmsee C,et al.The β2-adrenoceptor agonist denbuterol modulates Bcl-2,Bcl-xl and Bax protein expression following transient forebrain ischemia[J].Neurosci.1999,90(4):1255-1263
94.Cai J,Jones DP.Superoxide in apoptosis.Mitochondrial generation by cytochrome closs[J].J Biol Chem 1998,273(19):11401-11404.
95.Kruman I,Bruce-Kelle AJ,Bredesen D,et al Evidence that 4 hydroxynonenal mediates oxidativestress-induced neuronal apoptosis[J].Neurosci.1997,17(13):5089-5100.
96.Shimazaki K,Ishida A,Kawai N.Increase in bcl-2 oncoprotein and the tolerance to ischemia-induced neuronal death in the gerbil hipoocampus.Neurosci Res.1994;20(1):95-99
97.KirinoT.Ischem is tolerance[J].J Ceteb Blood Flow Metab.2002(22):1283
98.Ferrer I,Lpez R,Blanco R,et al.Bcl-2,bax,and bcl-x exprssion in the CA1 area of the hippocampus following transient forebrain ischemia in the adult gerbil [J].Exp Brain Res.1998,121(2):167-173.
99.Martinon JC,Dubois-Dauphin M,Staple JK,et al.Overexpression of Bcl-2intransgene mice protects neurons from naturally occurring cell death and experimental ischemia[J].Neuron,1994,13:1017-1030.
100.刘丹,韩威,张迪.不同时间针刺对局灶性脑缺血再灌注大鼠BCL-2、Bax蛋白表达的影响.中华中医药学刊.2007,25(3):622
101.Choi JM,Shin Hk,Kim KY,Lee JH,Hong KW.Neuroprotective effect of cilostazol against focal cerebral ischemia via antiapoptotic action in rats.J pharmacol Exp Ther.2002,300(3):787-793
102.曲友直,赵燕玲,秦怀洲,等.川芎嗪对脑缺血再灌注后神经细胞凋亡及其相关蛋白Fas-L表达的影响.中国中医急症.2007,16(5):581
103.卫立辛,钱其军.Fas介导凋亡的免疫学研究进展[J]。国外医学·生理病学与临床分册.1997,17(4):281
104.narl M,Hug H,Nagata S.Involvement of an ICE-like protcasc in Fas-,nediated apoptosis[J].Nature.1995,375(6526):78-81
105.Itoh N,Nagata s.A novel protein domain required for apoptosis[J].J Biol chem.1993,268(15):10932-10937
106.Ahbas AK.Eliominating dangerous lymplmoytes[J].Cell.1996,84:655-657
107.Matsuyama T,Hata R,Yamamoto Y,et al.Localization of Fas antigen mRNA induced in postischemic murine forebrain by in situ hybridization.Brain Res Mol Brain Res,1995,34(1):166-172
108.Frigerio S,Silei V,Ciusani E,et al.Madulation of Fas-ligand(Fas-L)on human mincrolial cellsia in vitro study[J].J Neurommunol Suppl.2000,105(2):109-114
109.Martin-Villalba A,Hahne M,Kleber S,et al.Therapeutic neutralization of CD95-ligand and TNF attenuates brain damage in stroke.Cell Death Differ.2001,8(7):679-686
110.郝久宽,于维东,白抚生,等.大鼠局灶性脑缺血再灌注后Fas表达与细胞凋亡关系的研究[J].脑与神经疾病杂志.2001,9(5):272-273
111.兰希发,姚文秀,郭阳,脑缺血再灌注后神经细胞凋亡的机制[J].中国临床康复.2003,7(19):2726-2727
112.孔立红,任康,周华,等.电针对脑缺血再灌注大鼠海马Fas蛋白表达的影响.中国中医急症.2006,15(11):1252
113.林海,刘煜,李柱一,等.局灶性脑缺血再灌注后白细胞介素-6、肿瘤坏死因子-α表达及川芎嗪对其表达的影响[J].中国临床康复.2002,6(7):968-969
114.黄建梅,唐一鹏,洪庆涛.抗呆1号对体外模拟脑缺血再灌注损伤海马神经元凋亡调控基因表达的影响[J].北京中医药大学学报.2002,25(1):38
115.Neumann-Haefelin T,Wiessner C,Vogel P,et al.Differential expression of the immediate early genes c-Fos,c-jun,junB,and NGFI-B in the rat brain following transient forebrain ischemia[J].Cereb Blood Flow Metab.1994,14(2):206-216 33.
116.Lee MC,Rho JL,Kim MK,et al.c-JUN expression and apoptotic cell death in kainate-induced temporallobe epilepsy[J].J Korean Med Sci.2001,16(5):649-656
117.Kokubo Y,Matson GB,Liu J et al.Correlation between changes in apparent diffusion coefficient and induction of heat shock protein,cell-specific injury marker expression,and protein synthesis on diffusion-weighted magnetic resonance images after temporary focal cerebral ischemia in rats[J].J Neurosurg.2002,96(6):1084-1093
118.Hata R,Maeda K,Hermann D,etal.Dynamics of regional brain metabolism and gene expression after middle cerebral artery occlusioninmice[J].J Cereb Blood Flow Metab.2000.20(2):306-315.
119.诸榕.脑缺血的细胞死亡:坏死或凋亡.中华神经科杂志[J].1998.31(5):301-304.
120.Garcia JH,Ye ZR,kiu KE.Delayed neuronal death in experimental ischemicstroke[M].In:Touetal.eds:Maturation phenomenon in cerebral ischemia.Berlin:Springer-Verlag.2002,25(6):267-273.
121.HarrisonW,Beyaert R,Declercq W,Vandenabeele P More than one way to ie:apoptosis and necrosis and reactive oxygen damage[J].Onco gene.1999,18(54):7719-7730
122.Morganti-Kossmann MC,Ranoan M,Stahcl PE,et al.Inflammatory response in axute rtaumatic brain injury,a double-edged sword[J].Currp Opin Crit Car.2002,8(2):101-105
123.Rothwell N.Interleukin-landneuronal injury:mechanis-ms,modification,and the rapeutic potential[J].Brain Behavlmmun.2003,17(3)147-152
124.Emerich DF,Dean RL3rd Bartus RT,etal The role of leukocytes following cerebral ischem is pathogenic variable or bystander reaction to emerging infarcts[J].Exp Neurol 2002,168-173
125.Liao SL,Chen W Y,Raung SL,Kuo JS,Chen CJ.Association of immune res-ponse and ischemic brain infarction in rat.Neuroreport.2001,12(9):1943-1947
126.张田,魏子峰.中医药对脑缺血再灌注脑损伤保护作用研究的新进展.华北煤炭医学院学报.2007,9(2):190
127.徐皓亮,冯亦璞.黏附分子与脑缺血后炎症[J].生理科学进展.2000,31(3):257
128.田金洲.血管性痴呆.北京:人民卫生出版社.2003:253
129.张莉莉,王景周,周华东,等.肿瘤坏死因子和细胞黏附分子在脑缺血再灌注损伤中的表达规律[J].中国临床康复.2002,6(19):2968-2969
130.陈邦国,毛庆菊,孔立红.电针对局灶性脑缺血再灌注大鼠缺血脑区细胞黏附分子表达的影响.湖北中医学院学报.2006,8(4):3
131.Frijus CJ,appelle LJ.Inflammatory cell adhesion molecules ischemic cerebrovascular disease[J].Stroke 2002,33(8):2115-2122
132.高伟,梁日欣,肖水庆,等.川芎内酯A预处理对大鼠离体心脏缺血再灌注所致血管内皮细胞损伤的保护作用[J].中国中药.2005,30(18):1448-1552.
133.Preckl B,ScJilack W,Comfcrc T.Effect of dantrolene in an in vivo and in vitro,model of myocardial reperfusion injury[J].Actar- Anaesthesiol-Scand.2000,44(2):194-198.
134.何志文,欧阳嶷,张强,等.老年急性血管病患细胞因子及其受体的研究.中华老年心脑血管病杂志.2001,3(5):322
135.向红兵,晋光荣,吴颢听,等.小鼠多发性脑梗死后脑组织内皮细胞ICAM-1表达的变化.东南大学学报(医学版).2004,23(5):295
136.Del Zoppo GJ.Microvascular responses to cerebral ischem is/in flamma tion Ann NYA Cad Sci.1997,823:132-147
137.Stanim irovic D,Shapiro A,Wang J.et al.The induction of ICAM-I in human cerebrom icrovascular endothelial cells(HCEC)by ischem ialike conditions promotes enhanced neutrophil/HCEC adhesion J Neuro immuno.1997,76(12):193-205
138.杨倩,吴家幂,陈绍兰.细胞间黏附分与脑缺血再灌注脑损伤.皖南医学院学报.2002,21(1):71-73
139.王伟,王雪松,阮旭中.海风藤新术脂素成分对缺血鼠脑细胞间黏附分了-1及其mRNA表达的影响.中华物理医学与康复杂志.2002,24(3):133-136
140.Brain SD,Williams TJ,Tippins JR,et al.Calcitonin gene-related peptide is apotent vasodiator.Nature.1985,313:54
141.Zhang KA.Oxidant stress regulation of ICAM-1 gene expression:differential activation and binding of the transcription factors AP-1.Int J Mol Med.1999,4(3):223-230.
142.SorianoA,Morgan BP,Tikkanen I,et al.Time course of complement activation and inhibitor expression after ischemic injury of rat myocardium[J].Am J Pathol.1999,144(2):1357-1358.
143.Vcmuganti R,Denlpscy R.IBcnvcn KK.Inhibition of intercellular aclhcsion molecule-1 Protein expression by antisense oligonuclectides is neurop rotective after transient middle cerebral artery occlusion in rat[J].Stroke 2004, 35:179-184.
144.BertiR,W illiams A.IMoffett JR,etal QQuantitative real-time RT-PCR analysis of inflammatory gone expression assocl-ated with ischem io-reperfusion brain injury[J],Cereb Blood Flwv Metab.2002,22:1068-1079.
145.Wang ZQ,ChenXC,YangGY,etal Inflammatory reaction after focal cerebral ischemia in mouse[J],Chin Med J Eng.2004,1(17):586-591.
146.Vemuganti R,Dempsey RJ,Bowen KK,et al.Inhibition of intercellular adhesion molecule-lprote in expression by antisense oligonucleotides is neuroprotective after transientmiddle cerebralartery occlusion in rat.Stroke.2004,35:179-184.
147.Berti R,Williams AJ,Moffett JR,et al.Quantitativereal-time RTPCR analysis of inflammatory gone expression associated with ischemia-reperfusion brain injury.)Cereb Blood Flow Metab.2002,22:1068-1079.
148.Love S,Barber R.Expression of P-selectin and intercellular adhesion molecule-1in humanbrain after focal infarction or cardiac arrest.Neuropathol Appl Neurobiol.2001,27:465-473.
149.孙丽莎,杨露春,程佩兰.达纳康对鼠脑缺血-再灌注后ICAM-1的影响.天津医药.2002,30(3):161-163
150.夏斌,邵福源,赵忠新,等.急性缺血性脑血管病患者血清可溶性黏附分子的变化.临床研究.2002,25(5):308-309
151.宋水江,郑晓红,闻树群,等.细胞间黏附分子1和碱性成纤维细胞生长因子在脑梗死中的作用.中华医学杂志.2002,82(21):1447-1449.
152.Konemoto.Y,Nakase H,Akita N,Sakaki J.Efects of anti- intercellular adhesion molecule-1 antibody on repeifusion injury ineluded by late repeifusion in the rat middle cerebral artery ocelusion models[J].Neurosygery.2002,51:1034-42
153.Bruce A,Boling W,Kindy MS,etal.Altered neuronal and microglial responses to excitotoxic and ischemic brain injury in mice lacking TNF receptors[J].Nat Med.1996,2(7):788-794.
154.吴玉泉,霍正禄.细胞粘附分子与脑梗死关系的研究进展.医学综述.2002,8(10):570-572
155.孙丽莎,杨露春,程佩兰.达纳康对鼠脑缺血-再灌注后ICAM-1的影响,天津医 药.2002,30(3):161-163
156.徐丽荣,马世彬,李澎涛,等.清开灵有效组份对局灶性脑缺血大鼠脑组织细胞间黏附分子表达的影响.中草药.2004,35(6):666-669
157.孙国兵,赵薛旭,李作汉,等.脑缺血再灌注大鼠细胞间黏附分子-1、血管细胞间黏附分子-1的表达及β-七叶皂甙钠的干预作用.临床神经病学杂志.2005,18(2):120-123
158.扈友庄,杨彬,刘东彪.葛根素对急性脑梗死患者血清sICAM-1,TNF-α水平的影响.中国医师杂志.2005,7(8):1135-1136
159.工亚贤,沈小苹,李明琦,等.刺五加注射液对大鼠局灶性脑缺血再灌注模型TNF-α和ICAM-1表达的影响.哈尔摈医科大学学报.2007,41(6):561
160.孔立,尤可,江涛,等.调气息风法对脑缺血大鼠ICAM-11L-1β及TNF-α表达的影响中国中医急症.2007,16(9):1098.
161.柳洪胜,张允岭,刘雪梅,等.清脑宣窍滴丸对大鼠局灶脑缺血再灌注炎症因子含量的影响,中华中医药杂志.2007,22(5):328
152.王玉升.电针对急性局灶性脑缺血模型大鼠的影响.中国针灸.1996,(9):34
153.司全明.电针对脑缺血大鼠体感诱发电位及脑便塞体积的影响.针刺研究.1998,(1):36
164.张秀花,孙世晓,徐博佳.井穴点刺出血对局灶性脑缺血大鼠脑梗塞体积影响的基础研究.针灸临床杂志.2004,20(12):47-48
165.周飞,李福风,程介士,等.电针对急性脑缺血大鼠局部脑血流和脑梗死体积的影响.上海针灸杂志.2003,22(5):3-6
166.裴海涛,郭壮丽.头穴针刺局灶性再灌注大鼠脑内TGF-β mRNA表达的调节.中国针灸.2003,23(12):739-741
167.张振强,刘丽娟,李建会.电针干预脑缺血再灌注大鼠梗死体积及脑内白细胞介素1β蛋白表达的变化,中国临床康复.2006,10(43):153-155
168.闫庆军.针刺人中对急性脑缺血大鼠海马rCBF神经元超微结构的影响.湖北中医学院学报.1999,1(1):28-30
169.许能贵,沈德凯,周逸平,等.电针对局灶性脑缺血大鼠发层体感诱发电位和细胞超微结构的影响.中医杂志.2001,42(6):342-346 30.
170.刘波,唐强,李静.针刺治疗缺血性脑损伤的实验研究.中国康复理论与实 践.2005,11(7):514-515
171.易玮,许能贵,汪帼斌,等.电针对局灶性脑缺血大鼠突触可塑性促进作用的实验研究.中国中西医结合杂志.2006,26(8):710-714
172.Kontos HA.Oxygen radicals in celebral ischemia:the 2001 Willis lecture[J].Stroke.2001,32:2712-2716
173.周国平,邓常青.手十二井穴放血抗兔实验性脑缺血机理的研究.针刺研究.1999,24(2):101
174.郑谅,周莹,李艳慧,等.针刺对中风过氧化脂质和抗氧化酶的影响.新中医.2001,33(6):42
175.许能贵,易玮,马勤耘.电针对大鼠局灶性脑缺血后神经元损伤保护作用的研究中国针灸.2000,20(4):237-240
176.陆任云,徐斌等.针刺对缺血再灌注脑组织形态结构和酶活性影响的实验研究.针刺研究.2002,27(1):5-9.
1177.辛随成,郭书文,王国华,针药结合对实验性脑缺血脑细胞凋亡及相关因素的影响.中国中医药信息杂志.2004,11(3):218-220
178.徐坚,陶陶,何燕,等.天麻及电针对大鼠脑缺血再灌注损伤的保护作用.中国脑血管病杂志.2004,1(5):221-224
179.许燕,陆任云,徐斌,等.针刺对脑缺血再灌注大鼠脑组织NOS及NO的影响[J].镇江医学院学报.1999,9(4):513-514
180.施静,张静,刘晓春,等.电针对局灶性脑缺血再灌注大鼠大脑皮层一氧化氮的影响[J].针刺研究.1999,24(4):268-273
181.张京钟,施静,刘晓春,等.电针对大鼠局灶性脑缺血后BAX,BCl-2表达的影响.中国组织化学和细胞化学杂志.2001,10(1):27
182.Jin GL,Su J.Ding SQ,Zhou DF.Theeffect of electroacupuncture on behavior and brain'smonoamine neurotransmitters of rat[J].Zhongguo Xingwei Yixue(Chin Behav Med Sci.2000,9(3):164-166
183.田菲.电针对缺血再灌注大鼠脑组织NO及自由基的影响[J].中国中医急症.1999,8(1):33
184.董裕.针刺对暂时性脑缺血脑内一氧化氮台成酶及神经细胞死亡的影响[J].钳刺研究.2000(1):8-13
185.许燕,等.针刺对脑缺血再灌注大鼠脑组织NOS及NO的影响.[J]镇江医学院学报.1993,9(4):513-514
186.郭义,胡利民,张严峻,等.手十二井穴刺络放血对实验性脑缺血大鼠缺血区细胞外液钙浓度影响的动态观察.针灸临床杂志.1999,15(6):48
187.马惠芳,孙华,任秀君,等.电针水沟与井穴对全脑缺血大鼠脑组织钙调素活性影响的对比研究[J].针灸研究.2002,27(2):102-104
188.许能贵,易玮,赖新生,等.电针对局造脑缺血大鼠脑细胞内Ca~(2+)含量的影响.中国中西医结合杂志,2002,22(4):295
189.金智秀,郝晋东,等.不同时间窗电针对急性局灶性脑缺血模型大鼠脑组织活性钙调素含量影响的研究.针刺研究.2003,28(3):178-181
190.金智秀,图娅,洪银珠,等.头针、体针和针药结合治疗对全脑缺血大鼠钙调素活性即刻影响的比较研究.针刺研究.2004,29(1):18-20 48.
191.霍则军,任秀君,刘青云.针刺不同穴组对脑缺血再灌注大鼠保护作用研究,山西中医.2004,20(1):47-48
192.姚凯,郭义,胡利民,等.针刺对大鼠脑缺血超早期脑细胞内外游离钙离子浓度的影响.中国中西医结合急救杂志.2005,12(5):303-305
193.施静,等.电针对脑缺血性神经元凋亡的影响[J].中国组织化学与细胞化学杂.1998,7:391
194.许能贵,马勤耘,侯思伟.电针对局灶性脑缺血大鼠兴奋氨基酸含量的影响.中国针灸.1997,17(7):431
195.郭景春,程介士.电针对脑缺血大鼠纹状体细胞外兴奋性氨基酸水平的影响.上海针灸杂志.2000,19(1):37-39.
196.邹军,张家维,赖新生等.电针对缺血性中风兴奋性氨基酸、c-fos及细胞凋亡影响.陕西中医.2002,23(8):760-761
197.角建瓴,赖新生.电针对局灶性脑缺血大鼠再灌流后四种氨基酸含量的影响.针刺研究.2003,28(3):170-173
198.甘平,郭景春,杨茹,等.针刺抗脑缺血损伤时脑内γ-氨基丁酸的作用.上海针灸杂志.2003,22(9):3-6
199.陈虎,蔡定芳.电针对局灶性脑缺血大鼠海马兴奋性氨基酸受体鲍影响.上海针灸杂志.2003,22(5):13-15
200.施静.针刺抗脑缺血性神经元凋亡作用与机制的研究.生理科学进展.1999,30(4):326-329
201.李成永,吴嘉容,沈国权,等推拿、针刺对急性脑缺血大鼠的脑保护作用.中医药信息.2003,20(6):44-45
202.李成永,李文金,苏维广,等.眼针对急性脑缺血大鼠的脑保护作用.辽宁中医杂志,2004,31(2):104-105
203.王红伟,徐华明.针刺对局灶性脑缺血再灌注大鼠脑细胞凋亡的影响.河南中医学院学报.2007,22(6):21
204.甘云波,黄光英,张明敏.针刺对大鼠局灶性脑缺血再灌注损伤后神经元凋亡的影响.山东医药.2007,47(15):24
205.唐伟,孙忠人,张力,等.钟刺预处理全脑缺血大鼠海马CA1区细胞凋亡和热休克蛋70mRNA的表达,中国临床康复.2005,9(37):90-92
206.唐伟,孙忠人,王卓尔,等.针刺预处理对大鼠脑缺血再灌注损伤的保护作用.中国中医药科技.2006,13(4):209-211
207.唐伟,孙忠人,王卓尔,等.针剌预处理对脑缺血再灌注损伤的保护作用.辽宁中医杂志.2006,33(5):627-628
208.孙忠人,唐伟,单丽莉,等.针刺诱导脑缺血耐受的实验研究.中国临床康复.2005,9(1):122-123
209.Jang MH,Shin MC,Lee TH,et al,Acupuncture sup-presses ischemia-induced I ncrease in c-Fos expression and apoptosis in the hippocampal CAI region in gerbils[J].Neu-rosci Lett.2003,347(1):5-8.
210.余晓慧.电针对局灶性脑缺血大鼠Caspase-3蛋白表达的影响.辽宁中医学院学报.2005,7(2):167
211.罗勇,董为伟.电针上调局灶性脑缺血再灌注时脑组织抗凋亡基因bel-2蛋白表达.中华物理医学与康复医学杂志.2000,22(1):53
212.邹军,赖新生,张家维,等.电针对缺血性中风细胞凋亡及Bel-2,P53的影响.四川中医.2002,(8):9
213.余晓慧.针刺对局灶性脑缺血大鼠脑细胞凋亡及Bcl-2蛋白表达的影响.针刺研究.2004,29(1):15-17
214.田代实,邓医宇,王光海,等.针刺预处理对大鼠局灶性脑缺血的抗细胞凋亡作用 第四军医大学学报.2004,25(1):27-29
215.孙忠人,张力,郑美华,等.针刺预处理对全脑缺血大鼠脑组织Bcl-2蛋白表达影响的实验研究.中医药学刊.2006,24(11):1976-1977
216.唐伟,王威,孙忠人,等.针刺预处理全脑缺血大鼠海马CA1区Bcl-2mRNA的表达.中国中医基础医学杂志.2007,13(2):156
217.孔立红,任婕,周华,等.电针对脑缺血再灌注大鼠海马Fas蛋白表达的影响.中国中医急症.2006,15(11):1252.
218.王利,张学勤,张勇,等.电针对大鼠脑局部缺血再灌注后c-fosmRNA表达的影响[J].中国针灸.2000 20(4):241-243
219.邹军,张家维,赖新生,等.电针对缺血性中风兴奋性氨基酸、c-fos及细胞凋亡影响.陕西中医.2002,23(8):760
226.王秀云,李积胜,郭义,等.手十二井穴刺络放血法对MCAO模型大鼠大脑皮层c-fos蛋白表达的影响.上海针灸杂志.2004,23(12):39-41152.
221.张春红,王舒,石学敏,等.醒脑开窍针法对脑梗死模型大鼠脑组织c-fos基因表达的影响.天津中医药,2004,21(3):210-213
222.孙忠人,唐伟,王威.针刺预处理诱导全脑缺血大鼠海马CA1区即刻早期基因c-fos mRNA及其蛋白的表达.中国临床康复,2006,10(19):125-127
223.王文远,赵建明,齐迎春.平衡针法对局灶性脑缺血大鼠血清TNF-α,stCAM-1的影响.临床军医杂志,2004,32(1):5-7
224.章薇,刘智,娄必丹,等.平衡针刺法对脑梗死患者IL-1和sICAM-1、sVCAM-1的影响[J].中国针灸.2005,25(3):214-216
225.刘玉珍,韩景献,姜文,等.醒脑开窍针法对脑缺血再灌注后细胞间黏附分子-1和P-选择素表达调节的实验研究.天津中医药.2005,22(6):462
226.苏红梅,朱广旗,欧阳冷星,等.针刺对大鼠脑缺血再灌注损伤后ICAM-1表达的影响.上海针灸杂志.2006,25(12):30
227.陈邦国,毛庆菊,孔立红.电针对局灶性脑缺血再灌注大鼠缺血脑区细胞黏附分子表达的影响.湖北中医学院学报.2006,8(4):3
228.孔立红,毛庆菊,陈邦国.电针对局灶性脑缺血再灌注大鼠细胞间黏附分子-1表达的影响.世界针灸杂志.2007,17(1):31
229.Zea-longa E,Weinstein PR,Carlson,et al.Reversible middle cerebral arteryocclusion without craniectomy in rat[J].Stroke 1989,20(1):84-91
230.Bederson JB.Et al.Rat middle cerebral artery occlusion using an intraluminal thread technique.Acta Neurchir.1991,109:126
231.高维滨.神经病针灸新疗法.人民卫生出版社.2002年12月第1版第1次印刷.
232.高碧霄,张跃全,等.十余年来百会研究概况.中医文献杂志.1997,(2):41-43
233.苗华,崔可密,卢岩,等.大鼠脑缺血模型的复制方法及评价.山东医药.2001,20(3):23-26.
234.杨元德.针灸治疗中风及后遗症近10年的文献概述.中国针灸,1992,(4):48-52.
235.关云谦,陈彪,周茗邹,等.尼龙线直径和硬度对大鼠线栓法脑缺血模型成功率的影响.中国医学科学院学报.2004,26(5):600.
236.Meyer R.Wcisscrt R.et al.Acute neuronal apoptosis in a rat modclof multiple selerosis J Neurosci,2001,21(16):6214-6220
237.Martin ZJ,Al-Abdulla NA,Bramrink.AM et al.Neurodegeneration in excitotoxicity,global cerebral ischemia,and target deprivation:aperspective on the contribution of apoptosis and necrosis.Brain Res Bul.1998,46:281-309.
238.霍则军,任秀君,刘青云.针剌不同穴组对脑缺血再灌注大鼠保护作用研究[J].西中医.2004,20(1):47
239.Martinou J-C Dubois IDauphin M,Staple JK,et al.Overexpression of bcl-2 in transgenic mice protects neurons from naturally occurring cell death and experimental ischemia Neuron.1994,13(4):1017-1013
240.Linnik MD Zahos P,Geschwind MD,et al.Expression of bcl-2 frema defect ive herpes sirr}lex virus-1 vector limits neuronal death in focalcerebral ischemia Stroke.1995,26(9):1670-1674
241.NagataS,GolsteinP.TheFas death factor.Science.1995,267(5203):1449-1456
242.刘勇,胡明均,何国厚.Fas/FasL的高表达在缺血性脑损伤中的意义.中国临床康复.2003,7(31):4184-4185
243.Martin-Villalba A,Herr I,Jeremias I,Hahne M,Brandt R,Vogel J,Schenkel J,Herdegen T,Debatin KM.CD95 ligand(Fast/Apo-1L)and tumor necrosisfactor-related apoptosis-inducing ligand mediate ischemia-induced apoptosis in neurons.J Neurosci.1999,19(10):3809-3817
244.Rosenbaum DM,Gupta G,D Amore J.Sihgh M,Weidenheim K,Zhang H,Kessler JA.Fas(CD95/Apo-1)plays a role in the pathophysiology of focal Cerebral ischemia.J Neurosci Res.2000,61(6):686-692
245.Zhang RL,et al.Brain Research.1995,682(1):182
246.Sobel RA,Mitebell ME,Fondren G.Intercellular adhesion molecule-1(ICAM-1)in cellular immune reasons in the human central nervous system.Am J Pathol.1991,136:1309-1316
247.Wang X,Feuerstein GZ.Induced expression of adhesion molecules following focalbrain ischemia.J Neurotrauma.1995,12(5):825-832
248.Clark WM,Lauten JD,Lessov N,et al.Time course of ICAM-1 expression andlcukocytc subsct infiltration in rat forcbrain ischcmia.Mol Chcm Neuropathol 1995,26(3):213-230
249.Zhang RL,Chopp M,Zaloga C,et al.The temporal profiles of ICAM-1 protein andmRNA expression after transient MCA occlusion in the rat.Brain Res.1995,682(1-2):182-188
250.Kitagawa K,et al.Deficiency of intercellular adhesion molecular-1attenuates microcirculatory disturbance and infarction size in focal ischemia.J Cereb Blood Flow Metab.1998.18(12):1336
251.张莉莉,等.大鼠脑缺血再灌注区细胞间粘附分子-1表达与白细胞浸润的实验研究.中国危重病急救医学.2000,12(5):264
252.沈德凯,等.电针对局灶性脑缺血大鼠神经元损伤保护作用的病理组织学研究。中国中医药科技.1998,5(5):269