广藿香油抗病毒的物质基础研究
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摘要
目的：通过广藿香油体外抗流感病毒、呼吸道合胞病毒、腺病毒、柯萨奇病毒及单纯疱疹病毒的效果,筛选出敏感病毒株,研究广藿香油体外抗病毒的作用方式,并通过体内验证广藿香油抗病毒效果。从广藿香油主要物质成分广藿香醇和广藿香酮对以上5种病毒的效果,初步探讨广藿香油抗病毒的药效物质基础及作用原理。
方法：建立流感病毒(HINI)感染MDCK细胞、呼吸道合胞病毒(RSV)和腺病毒(ADV)感染Hep-2细胞、柯萨奇病毒(CVB3)感染Hela细胞模型和单纯疱疹病毒(HSV-I)感染Vero细胞模型,以细胞病变效应(CPE)、噻唑蓝(MTT)法作为观察和检测指标,观察不同浓度广藿香油对细胞的毒性及体外抗病毒效果,筛选敏感病毒株,根据病毒复制周期特点研究药物作用方式,并用最敏感病毒株腹腔注射小鼠建立体内致病模型,观察广藿香油经腹腔注射给药对模型小鼠的一般情况、生存率、心肝肺肾脏器指数,病理切片和血清生化指标的影响,验证广藿香油体内抗病毒效果。另外从广藿香油主要物质成分广藿香醇和广藿香酮对细胞的毒性及体外抗病毒效果,筛选出敏感病毒株,研究药物与病毒的作用方式,采用常规PCR和Real-time PCR的方法定位药物对病毒的作用靶点,初步探讨广藿香油抗病毒的药效物质基础及作用原理。
结果：广藿香油及其主要单体成分广藿香醇和广藿香酮对MDCK、Hep-2、Vero和Hela细胞的毒性结果都基本一致,广藿香油、广藿香醇和广藿香酮对4种细胞的TC50浓度分别为0.101mg/ml、0.125mg/ml和0.071mg/ml,TCo浓度分别为0.092mg/ml、0.031mg/ml和0.063mg/ml;广藿香油、广藿香醇和广藿香酮能不同程度的抑制H1N1、CVB3和ADV所致的细胞病变,对RSV的抑制作用低或无作用,对HSV-1几乎无抑制作用。广藿香油、广藿香醇和广藿香酮对H1N1的IC50分别为0.088mg/ml、0.031mg/ml和0.063mg/mI,治疗指数(TI)为1.13、4.0和1.13；对的IC50分别为0.08mg/ml、0.063mg/ml和0.067mg/ml,TI为1.25、2.0和1.06；对ADV的IC50分别为0.084mg/ml、0.063mg/ml和0.063mg/ml,TI为1.20、4.0和1.13。广藿香油和广藿香醇对病毒的作用优于广藿香酮。广藿香油和广藿香醇对病毒的直接作用效果都优于抗病毒吸附和抗病毒生物合成,而且在直接作用方面,同一浓度下广藿香油对CVB3的抑制率高于H1N1和ADV,广藿香醇对ADV的抑制率高于H1N1和CVB3。PCR和Real-time PCR结果显示广藿香醇对ADV病毒的Hexon基因有一定的破坏作用。广藿香油腹腔注射给药LD50为4.441mg/g,95%预测区间为(3.768,5.317mg/g)。广藿香油对CVB3腹腔注射Balb/c小鼠建立的病毒性心肌炎动物模型有一定治疗作用,其中0.096g/kg浓度组的小鼠存活率、心脏病变缓解程度都高于阳性药物组,而且对肝肾等脏器有一定保护作用,能增加SOD活性,明显降低MDA和TNF-α水平,会略微降低LDH和CK-MB水平。
结论：在体外抗病毒实验中,广藿香油显示出抗柯萨奇病毒、腺病毒和甲型流感病毒的作用,对呼吸道合胞病毒和单纯疱疹病毒几乎无作用,其中对柯萨奇病毒的抑制作用最明显,同时对柯萨奇病毒引起的体内致病模型也有治疗效果,说明广藿香油体内外具有抗病毒效果。广藿香油的主要单体成分广藿香醇和广藿香酮也有抗病毒作用,其中广藿香醇的抗病毒效果明显优于广藿香酮,在抗腺病毒方面作用最明显,作用靶点以腺病毒负责翻译衣壳蛋白的Hexon基因为主。
Obejctive:Observing the effect of patchouli oil against influenza virus, respiratory syncytial virus, adenovirus, Coxsackie virus and herpes simplex virus in vitro. Screening the sensitive viruses to research the antivirus action of the drug. Verify the antiviral effect of Patchouli oil on viral animal model in vivo. Investigate the antivirus effect of the tow main components of patchouli oil which are patchouli alcohol and patchoulenone to discussing the antiviral pharmacodynamic substance foundation and action principle of patchouli oi.
Methods:Establish models of influenza virus (H1N1) infecting MDCK cell, respiratory syncytial virus (RSV) and adenovirus (ADV) infecting Hep-2cell, Coxsackie virus (CVB3) infecting Hela cell and herpes simplex virus (HSV-1) infecting Vero cell respectively. Use CPE and MTT as the observation and detection methods, to investigate the drug toxicity to the cells and the antiviral effect of different concentrations of patchouli oil, screeing the sensitive viruses to the drug. Researching the drug action based on the virus replication characteristic. Establish the viral infected mice model with the most sensitive virus according to intraperitoneal injection, observing the influences of the model general condition, survival rate, heart,liver,lung and kidney index, pathological sections and serum biochemical index by patchouli oil with intraperitoneal injection. Verifying the antiviral effect of patchouli oil in vivo. On the other hand, investigate the drug toxicity to the cells and the antiviral effect of different concentrations of patchouli alcohol and patchoulenone which are the main mate composition of patchouli oil, screeing the sensitive viruses, researching the drug action to virus. Using normal PCR and Real-time PCR to locate the target of drug action to virus. Explore the antiviral pharmacodynamic substance foundation of anti-virus and principle of patchouli oil.
Results:The cytotoxicity of Patchouli oil, patchouli alcohol and patchoulenone to MDCK, Hep-2, Vero and Hela cells are basically identical. The TC50of Patchouli oil, patchouli alcohol and patchoulenone to the cells are0.101mg/ml,0.125mg/ml and0.071mg/ml, the TC0are0.092mg/ml,0.031mg/ml and0.063mg/ml. Patchouli oil, patchouli alcohol and patchoulenone could inhibit the H1N1, CVB3and ADV virus induced cell lesion in different degrees, there's no inhibition or very low on RSV and no inhibition on HSV-1. The IC50of Patchouli oil, patchouli alcohol and patchoulenone to H1N1are0.088mg/ml,0.031mg/ml and0.063mg/ml,the therapeutic index (TI) is1.13,4.0and1.13; to CVB3are0.08mg/ml,0.063mg/ml and0.067mg/ml, TI are1.25,2.0and1.06; to ADV are0.084mg/ml,0.063mg/ml and0.063mg/ml,TI are1.20,4.0and1.13. Patchouli oil and patchouli alcohol are better than patchoulenone on the role of antivirus.Patchouli oil and patchouli alcohol on the direct action of the virus is superior to the anti-virus adsorption and antiviral biosynthesis. And in direct action, Patchouli oil on CVB3inhibition rate is higher than H1N1and ADV inhibition and patchouli alcohol on ADV inhibition rate is significantly higher than H1N1and CVB3inhibition under the same concentration. The result of PCR and Real-time PCR show that patchouli alcohol could damage the Hexon gene of ADV. The LD50of patchouli oil on mouse by intraperitoneal injection is4.441mg/g, the95%prediction interval is3.768,5.317mg/g. Patchouli oil has a certain therapeutic effect on viral myocarditis mice, the concentration group of0.096g/kg is higher than the positive drup group in the field of survival rate and cardiac remission, and has a certain protective effect on liver and kidney, can increase the SOD activity, decrease the MDA and TNF-α levels,slightly lower LDH and CK-MB level.
Conclusion:In vitro antiviral experiment, patchouli oil show the ability of anti-Coxsackie virus, anti-adenovirus, anti-influenza A virus except anti-respiratory syncytial virus, the anti-Coxsackie virus effect is superior than others, meanwhile patchouli oil has a good antagonistic effect on Viral myocarditis mice model injected by CVB3in vivo, this means patchouli oil has antiviral effect in vivo and in vitro. Patchouli alcohol and patchoulenone which are the main monomer components of patchouli oil show antiviral effect, patchouli alcohol is better than patchoulenone. Patchouli alcohol is obviously in anti-adenovirus on the target of Hexon gene which is responsible for translation capsid protein of adenovirus.
方法：建立流感病毒(HINI)感染MDCK细胞、呼吸道合胞病毒(RSV)和腺病毒(ADV)感染Hep-2细胞、柯萨奇病毒(CVB3)感染Hela细胞模型和单纯疱疹病毒(HSV-I)感染Vero细胞模型,以细胞病变效应(CPE)、噻唑蓝(MTT)法作为观察和检测指标,观察不同浓度广藿香油对细胞的毒性及体外抗病毒效果,筛选敏感病毒株,根据病毒复制周期特点研究药物作用方式,并用最敏感病毒株腹腔注射小鼠建立体内致病模型,观察广藿香油经腹腔注射给药对模型小鼠的一般情况、生存率、心肝肺肾脏器指数,病理切片和血清生化指标的影响,验证广藿香油体内抗病毒效果。另外从广藿香油主要物质成分广藿香醇和广藿香酮对细胞的毒性及体外抗病毒效果,筛选出敏感病毒株,研究药物与病毒的作用方式,采用常规PCR和Real-time PCR的方法定位药物对病毒的作用靶点,初步探讨广藿香油抗病毒的药效物质基础及作用原理。
结果：广藿香油及其主要单体成分广藿香醇和广藿香酮对MDCK、Hep-2、Vero和Hela细胞的毒性结果都基本一致,广藿香油、广藿香醇和广藿香酮对4种细胞的TC50浓度分别为0.101mg/ml、0.125mg/ml和0.071mg/ml,TCo浓度分别为0.092mg/ml、0.031mg/ml和0.063mg/ml;广藿香油、广藿香醇和广藿香酮能不同程度的抑制H1N1、CVB3和ADV所致的细胞病变,对RSV的抑制作用低或无作用,对HSV-1几乎无抑制作用。广藿香油、广藿香醇和广藿香酮对H1N1的IC50分别为0.088mg/ml、0.031mg/ml和0.063mg/mI,治疗指数(TI)为1.13、4.0和1.13；对的IC50分别为0.08mg/ml、0.063mg/ml和0.067mg/ml,TI为1.25、2.0和1.06；对ADV的IC50分别为0.084mg/ml、0.063mg/ml和0.063mg/ml,TI为1.20、4.0和1.13。广藿香油和广藿香醇对病毒的作用优于广藿香酮。广藿香油和广藿香醇对病毒的直接作用效果都优于抗病毒吸附和抗病毒生物合成,而且在直接作用方面,同一浓度下广藿香油对CVB3的抑制率高于H1N1和ADV,广藿香醇对ADV的抑制率高于H1N1和CVB3。PCR和Real-time PCR结果显示广藿香醇对ADV病毒的Hexon基因有一定的破坏作用。广藿香油腹腔注射给药LD50为4.441mg/g,95%预测区间为(3.768,5.317mg/g)。广藿香油对CVB3腹腔注射Balb/c小鼠建立的病毒性心肌炎动物模型有一定治疗作用,其中0.096g/kg浓度组的小鼠存活率、心脏病变缓解程度都高于阳性药物组,而且对肝肾等脏器有一定保护作用,能增加SOD活性,明显降低MDA和TNF-α水平,会略微降低LDH和CK-MB水平。
结论：在体外抗病毒实验中,广藿香油显示出抗柯萨奇病毒、腺病毒和甲型流感病毒的作用,对呼吸道合胞病毒和单纯疱疹病毒几乎无作用,其中对柯萨奇病毒的抑制作用最明显,同时对柯萨奇病毒引起的体内致病模型也有治疗效果,说明广藿香油体内外具有抗病毒效果。广藿香油的主要单体成分广藿香醇和广藿香酮也有抗病毒作用,其中广藿香醇的抗病毒效果明显优于广藿香酮,在抗腺病毒方面作用最明显,作用靶点以腺病毒负责翻译衣壳蛋白的Hexon基因为主。
Obejctive:Observing the effect of patchouli oil against influenza virus, respiratory syncytial virus, adenovirus, Coxsackie virus and herpes simplex virus in vitro. Screening the sensitive viruses to research the antivirus action of the drug. Verify the antiviral effect of Patchouli oil on viral animal model in vivo. Investigate the antivirus effect of the tow main components of patchouli oil which are patchouli alcohol and patchoulenone to discussing the antiviral pharmacodynamic substance foundation and action principle of patchouli oi.
Methods:Establish models of influenza virus (H1N1) infecting MDCK cell, respiratory syncytial virus (RSV) and adenovirus (ADV) infecting Hep-2cell, Coxsackie virus (CVB3) infecting Hela cell and herpes simplex virus (HSV-1) infecting Vero cell respectively. Use CPE and MTT as the observation and detection methods, to investigate the drug toxicity to the cells and the antiviral effect of different concentrations of patchouli oil, screeing the sensitive viruses to the drug. Researching the drug action based on the virus replication characteristic. Establish the viral infected mice model with the most sensitive virus according to intraperitoneal injection, observing the influences of the model general condition, survival rate, heart,liver,lung and kidney index, pathological sections and serum biochemical index by patchouli oil with intraperitoneal injection. Verifying the antiviral effect of patchouli oil in vivo. On the other hand, investigate the drug toxicity to the cells and the antiviral effect of different concentrations of patchouli alcohol and patchoulenone which are the main mate composition of patchouli oil, screeing the sensitive viruses, researching the drug action to virus. Using normal PCR and Real-time PCR to locate the target of drug action to virus. Explore the antiviral pharmacodynamic substance foundation of anti-virus and principle of patchouli oil.
Results:The cytotoxicity of Patchouli oil, patchouli alcohol and patchoulenone to MDCK, Hep-2, Vero and Hela cells are basically identical. The TC50of Patchouli oil, patchouli alcohol and patchoulenone to the cells are0.101mg/ml,0.125mg/ml and0.071mg/ml, the TC0are0.092mg/ml,0.031mg/ml and0.063mg/ml. Patchouli oil, patchouli alcohol and patchoulenone could inhibit the H1N1, CVB3and ADV virus induced cell lesion in different degrees, there's no inhibition or very low on RSV and no inhibition on HSV-1. The IC50of Patchouli oil, patchouli alcohol and patchoulenone to H1N1are0.088mg/ml,0.031mg/ml and0.063mg/ml,the therapeutic index (TI) is1.13,4.0and1.13; to CVB3are0.08mg/ml,0.063mg/ml and0.067mg/ml, TI are1.25,2.0and1.06; to ADV are0.084mg/ml,0.063mg/ml and0.063mg/ml,TI are1.20,4.0and1.13. Patchouli oil and patchouli alcohol are better than patchoulenone on the role of antivirus.Patchouli oil and patchouli alcohol on the direct action of the virus is superior to the anti-virus adsorption and antiviral biosynthesis. And in direct action, Patchouli oil on CVB3inhibition rate is higher than H1N1and ADV inhibition and patchouli alcohol on ADV inhibition rate is significantly higher than H1N1and CVB3inhibition under the same concentration. The result of PCR and Real-time PCR show that patchouli alcohol could damage the Hexon gene of ADV. The LD50of patchouli oil on mouse by intraperitoneal injection is4.441mg/g, the95%prediction interval is3.768,5.317mg/g. Patchouli oil has a certain therapeutic effect on viral myocarditis mice, the concentration group of0.096g/kg is higher than the positive drup group in the field of survival rate and cardiac remission, and has a certain protective effect on liver and kidney, can increase the SOD activity, decrease the MDA and TNF-α levels,slightly lower LDH and CK-MB level.
Conclusion:In vitro antiviral experiment, patchouli oil show the ability of anti-Coxsackie virus, anti-adenovirus, anti-influenza A virus except anti-respiratory syncytial virus, the anti-Coxsackie virus effect is superior than others, meanwhile patchouli oil has a good antagonistic effect on Viral myocarditis mice model injected by CVB3in vivo, this means patchouli oil has antiviral effect in vivo and in vitro. Patchouli alcohol and patchoulenone which are the main monomer components of patchouli oil show antiviral effect, patchouli alcohol is better than patchoulenone. Patchouli alcohol is obviously in anti-adenovirus on the target of Hexon gene which is responsible for translation capsid protein of adenovirus.
引文
[1]董永绥,方峰.病毒性疾病的诊断和治疗[J].临床儿科杂志,2002,20(5)：261-263.
[2]李莹.中药抗呼吸道病毒感染药理作用研究进展[J].山西中医,2007,23(4)：71-72.
[3]李燕宁,吴金勇,周明,等.中成药抗呼吸道病毒感染的实验研究进展[J].山东医药,2011,15(51)：111-112.
[4]金奇.医学分子病毒学[M].北京：科学出版社,2011.
[5]Dawood FS, Jain S, Finelli L,et al. Emergence of a novel swin-origin influenza A (H IN 1) virus in humans[J]. New Engl J Med,2009,360(25):2605-2615.
[6]雷达,艾立新,邓红樱,等.流感大流行特征启示甲型H1N1流感防控策略[J].中国国境卫生检疫杂志,2009,32(3)：145-150.
[7]Salom D, Hill BR, Lear JD,et al. pH-dependent tetramerization and amantadine binding of the transmembrane helix of M2 from the influenza A Virus [J]. Biochemistry,2000,39(46):14160-14170.
[8]Jefferson T, Deeks JJ, Demicheli V,et al. Amantadine and rimantadine for preventing and treating influenza A in adults[J]. Cochrane Database Syst Rev,2004,(3):1169.
[9]Saito R, Sakai T, Sato I,et al. Frequency of amantadine-resistant influenza A viruses during two seasons featuring co-circulation of H1N1 and H3N2[J]. Journal of Clinical Microbiology,2003,41(5):2164-2165.
[10]Astrahan P, Kass I, Cooper MA,et al. A novel method of resistance for influenza against a channel blocking antiviral drug[J]. Proteins,2004,55(2):251-257.
[11]Gubareva LV. Molecular mechanisms of influenza virus resistance to neuraminidase Inhibitors[J]. Virus Research,2004,103(2):199-203.
[12]Matrosovich M, Klenk HD. Natural and synthetic sialic acid-containing inhibitors of influenza virus receptor binding[J]. Rev Med Virol,2003,13(2):85-97.
[13]Haynes LM, Jones LP, Barskey A,et al. Enhanced disease and pulmonary eosinophilia associated with formalin-inactivated respiratory syncytial virus vaccination are linked to G glycoprotein CX3C-CX3CR1 interaction and expression of substance P[J]. J Virol,2003,77(18):9831-9844.
[14]耿学辉,朱汝男,孙晓鸥,等.我国部分地区呼吸道台胞病毒分离株的亚型鉴定[J].中华儿科杂志,1997,35(7)：405-406.
[15]杨占秋,余宏.临床病毒学[M].北京：中国医药科技出版社,2000.103-105.
[16]Matsushima Y, Shimizu H, Kano A,et al. Novel human adenovirus strain, Bangladesh [J]. Emerg Infetc Dis,2012,18(5):846-848.
[17]谭耀驹,谢菲.腺病毒分型诊断研究进展[J].广东医学,2008,29(1)：156-157.
[18]曾军.流感样疾病的诊断和鉴别诊断[J].中华医学信息导报,2003,18(22)：15.
[19]van Tol MJ, Claas EC, Heemskerk B,et al. Adenovirus infection in children after allogeneic stem cell transplantation:diagnosis, treatment and immunity[J]. Bone Marrow Transplant,2005,35(1):573-576.
[20]陆权.儿科常见病毒性呼吸道感染的病因治疗[J].世界临床药物,2003,24(7)：398-403.
[21]Lei Shang, Zhangyi Qu, Lihua Sun,et al. Astragaloside IV inhibits adenovirus replication and apoptosis in A549 cells in vitro.J Pharm Pharmacol,2011,63:688-694.
[22]Nevels M, Tauber B, Spruss T, et al. "Hit-and-Run" transformation by adenovirus oncogenes [J]. J Virol, 2001,75(7):3089-3094.
[23]Lenaerts L, Naesens L. Antiviral therapy for adenovirus infections[J].Antiviral Res, 2006,71(2-3):172-180.
[24]杨英珍,金佩英,郭棋,等.实验性小鼠急性Coxsackie B病毒性心肌炎的研究[J].病毒学杂志,1989,3：233-238.
[25]Andreoletti L, Hober D, Becquart P,et al. Experimental CVB3-induced chronic myocarditis in two murine strains:evidence of interrelationships between virus replication and myocardial damage in persistent cardiac infection[J]. J Med Virol,1997,52(2):206-214.
[26]陈鸿珊.抗病毒药物及其研究方法[M].北京：化学工业出版社,2006：35.
[27]Matsumori A. Lessons from animal experiments in myocarditis. Herz,1992,17(2):107-111.
[28]郝静,王一飞,张美英,等.地不容粗提物体外抗柯萨奇B-3病毒活性作用的实验研究[J].汀苏中医药,2008,(9)：83-84.
[29]Fohlman J, Paukson K, Morein B,et al. High yield production of an inactivated Coxsackie B3 adjuvant vaccine with protective effect against experimental myocarditis[J]. Scand J Infec Dis Suppl,1993,88:103-108.
[30]Frazer JK, Jackson DG, Gaillard JP,et al Identification of centerin:a novel human germinal center B cell restricted serpin[J]. Eur J Immunol,2000,30(10):3039-3048.
[31]王志玉,王桂亭,许洪芝,等.大黄醇提液的抗疱疹病毒的作用[J].中国中药杂志,1996,21(6)：364.
[32]解光艳,程惠娟.中药抗单纯疱疹病毒实验研究进展[J].安徽中医学院学报,2011,30(3)：78-80.
[33]汪群水,周雪群.五种中药制剂体外抗呼吸道病毒效果及细胞毒性的研究[J].全科医学临床与教育,2009,7(3)：226-228.
[34]Wyde PR, Moore-Poveda DK, O'Hara B,et al. CL387626 exhibits marked and unusual antiviral activity against respiratory syncytial virus in tissue culture and in cotton rats. Antiviral Res,1998,38:31-42.
[35]Lewinsohn DM, Bowden RA, Mattson D,et al. Phase I study of intravenous ribavirin treatment of respiratory syncytial virus pneumonia after marrow transplantation[J]. Antimicrob Agents Chemother,1996,40(11):2555-2557.
[36]汪永江,俞建,时梳民等.中药退热微型灌剂抗病毒作用研究[J].上海中医药杂志,2001,7(3)：41-42.
[37]Moler FW, Steihnart CM, Ohmit SE,et al. Effectiveness of ribavirin in otherwise well infants with respiratory syncytial virus-associated respiratory failure[J]. J Pediatr,1996,128(3):422-428.
[38]汉·张仲景.仲景全书·伤寒论(第4版)[M].北京：中医古籍出版社.1997.
[39]宋·杨士赢.仁斋直指附遗方论[M].台北：台北新文丰出版公司.1982.
[40]宋·陈师文,等.太平惠民和剂局方[M].北京：人民卫生出版社.1959.
[41]元·朱丹溪.丹溪心法[M].上海：上海科学技术出版社.1959.
[42]干振华,李金恒.中药抗病毒药理作用的研究进展[J].医学研究生学报,2007,20(12)：1290-1293.
[43]王玮,高永翔.中药抗病毒研究进展[J].现代中西医结合杂志,2008,7(17)：2753-2754.
[44]李英杰.中西医治疗流感高热疗效分析[J].现代中西医结合杂志,2001,10(3)：214.
[45]王英燕,王海.抗病毒中药的研究进展[J].中医药学报,2006,34(1)：25.
[46]喻凯,户上泰彦.392种植物提取成分的抗病毒活性研究[J].华西药学杂志,2004,19(3)：186-1 88.
[47]Cowan MM. Plant products as antimicrobial agents[J]. Clin Microbiol Rev, 1999(10):564-582.
[48]郭洁文,杨振淮,潘竞锵,等.蛇葡萄属植物抗病毒、抑菌和抗炎镇痛作用的研究概况[J].中医研究,2004,17(5)：57-59.
[49]胡嵘,张辉,徐风英,等.抗病毒动物药研究进展[J].吉林中医药,2003,12,23(12)：56-58.
[50]Bonn D. Green coffee beans may yield new class of anti-HIV-1 agents Lancet,1998,352(9133):1039-1042.
[51]Tagaya E, Tamaoki J, Kawatani K,et al. Inhibition of airway smooth muscle tone by Chinese herbal medicines[J]. Euro Respir J,2000,16(6):1123-1128.
[52]王海颖.中药与免疫的研究概况[J].中医研究,2000,13(5)：41-43.
[53]梁丽,徐磁海.中草药抗炎抗菌抗病毒成分的研究[J].长春中医学院学报,1996,15(2)：47-48.
[54]徐国钧,王强.中草药彩色图谱[M].福州：福建科学技术出版社,2006.
[55]中国医学科学院药物研究所.中药志(第4册)[M].北京：人民卫生出版社,1988.128.
[56]吴友根,郭巧生,郑焕强.广藿香本草及引种历史考证的研究[J].中国中药杂志,2007,32(20)：2114-2117,2181.
[57]国家药典委员会.中华人民共和国药典(一部)[S].北京：化学工业出版社,2003.3.
[58]年忠梅,丛浦珠.藿香的研究进展[J].湖北中医杂志,1990,1：44-47.
[59]唐慎微.重修政和经史证类备用本草[M].北京：人民卫生出版社,1957.309.
[60]陶弘景.名医别录(辑校本)[M].尚志钧辑校.北京：人民卫生出版社,1986.64.
[61]任守忠,靳德军,张俊清,等.广藿香药理研究进展[J].中国现代中药,2006,8(8)：27.
[62]鲁建江,王莉,陈宏伟,等.微波法提取藿香中的挥发油[J].ITCM,2001,18(5)：40.
[63]李建春,傅莺,孙玉明.广藿香挥发油β-环糊精包合工艺的研究[J].湖北中医杂志,2001,23(4)：52.
[64]薛漓.广藿香及其混淆品广防风的鉴别[J].中草药,2001,32(5)：460,461.
[65]肖省娥,贺红,徐鸿华,等.广藿香组织培养与植株再生研究[J].中药材,2001,24(6)：391,392.
[66]张广文,马祥全,苏镜娱,等.广藿香中的黄酮类化合物[J].中草药,2001,32(10),：871-874
[67]罗集鹏,冯毅凡,郭晓玲,等.不同采收期对广藿香产量及挥发油成分的影响[J].中药材,2001,24(5)：316,317.
[68]何冰,陈小夏,罗集鹏,等.广藿香去油部分的5钟不同极性提取物对胃肠道的影响[J].中药材,2001,24(6)：422,423.
[69]刘卓妍,徐法,张进,等.气相色谱法测定小儿广朴止泻口服液中广藿香醇含量[J].中国医院药学杂志,2001：21(11)：672,673.
[70]李立忠,胡朝凤,全载哲.藿香中广藿香酮的分离及鉴定[J].中南民族大学学报,2005,24(1)：14-16.
[71]吴周和,刘建成,吴小刚.藿香中天然防腐剂的提取方法及其抑菌作用研究[J].中国调味品,2004(8)：18-20.
[72]莫小路,严振,王玉生,等.广藿香精油对植物病原真菌的抑菌活性研究[J].中药材,2004,27(11)：805-807.
[73]刘亮锋,黄晓丹,蔡大可,等.广藿香油及藿香油的研究概况[J].中国中医药信息杂志,2009,16(2)：100-102.
[74]刘世增,杨吉成,顾振纶.香菊感冒冲剂的药理作用研究[J].中成药,1997,19(3)：25.
[75]高相雷,熊盛,王一飞,等.广藿香三种有效部位体外抗柯萨奇病毒B3作用的初步研究[J].中成药,2009,32(5)：261-264.
[76]贾旭玲,徐三,施芬.广藿香的研究进展[J].中华中医药学刊,2010,11(28)：2442-2444.
[77]肖培根.新编中药志(第三卷)[M].北京：化学工业出版社,2001.16.
[78]国家药典委员会.中国药典(Ⅰ部)[S].北京：中国医药科技出版社,2010：42.
[79]Xian YF, Li YC, Ip SP,et al. Anti-inflammatory effect of patchouli alcohol isolated from Pogostemonis Herba in LPS-stimulated RAW264.7 macrophages[J]. Exp Ther Med,2011,2:545-555.
[80]Kongkathip N, Samang P, Kongkathip B,et al. Development of patchouli extraction with quality control and isolation of active compounds with antibacterial activity[J]. Kasetsart J.(Nat.Sci.) 2009,43:519.
[81]Betty CR, Gregg Henderson, Ying Yu,et al. Toxicity and repellency of patchouli oil and patchouli alcohol against formosan subterranean termites coptotermes formosanus shiraki[J]. J.Agric.Food Chem,2003,51:4585.
[82]黄晓舞,白林,徐风华,等.广藿香醇对β淀粉样蛋白神经毒性的抑制作用[J].解放军药学学报,2008,24(4)：338.
[83]杜一民.广藿香的化学成分及其药理研究进展[J].中国新药与临床药理,1998,9(4)：23-25.
[84]彭成,代敏,万峰.藿香油在制备治疗细菌感染性疾病的药物中的用途[专利]2011.5.18.
[85]Mosmann T. Rapid colorimetric assay for cellular growth and survival:application to proliferation and cytotoxicity assays[J]. Immunol Methods,1983,65:55-63.
[86]中国医学科学院流行病防治研究所编.常见病毒实验技术[M].北京：科学出版社,1978.34.
[87]中华人民共和国卫生部药政局.抗病毒药物药效指导原则[S].新药(西药)临床前研究指导原则汇编,1993.
[88]Madisch I, Harste G, Pommer H,et al. Phylogenetic analysis of the main neutralization and hemagglutination determinants of all human adenovirus prototypes as a basis for molecular classification and taxonomy[J]. J. Viro,2005,79(24):15265-15276.
[89]沈丕安.中医抗病毒感染的治法和体会[J].世界临床药物,2007,28(11)：646-650.
[90]黄家诏.试析外感病辨治中的动态思维方法[J].江苏中医药,2006,27(11)：11.
[91]张静,杨维中,郭元吉,等.中国2001-2003年流行性感冒流行特征分析[J].中华流行病学杂志,2004,25(6)：461.
[92]杨京华,许尤佳,阳荣秀,等.120例小儿病毒性肺炎常见病原体与中医证候的关系研究[J].四川中医,2006,24(3)：78
[93]欧爱华,罗翌,严夏,等.SARS与急性上呼吸道感染中医证候分型及指标数量化方法的探讨[J].中国卫生统计,2006,23(4)：309.
[94]张于华.从“因地制宜”看区域地理环境和“中医外感病”的关系[J].中华现代中西医杂志,2005,3(12)：1105.
[95]李梦东.实用传染病学第2版[M].北京：人民出版社1998.18.
[96]Yaolan Li,Linda S,M.Ooil,et al. Antiviral Activities of Medicinal Herbs Traditionally Used in Southern Mainland China[J].Phytother Res,2004,18:718-722.
[97]胡天佑.具有抗病毒作用的中草药应用研究[J].中国处方药,2004,8(29)：69,70.
[98]史利卿.抗呼吸道病毒中药的实验研究述评[J].中国中医基础医学杂志,1998,4(12)：54,55.
[99]曲敬来,高雪,周岁锋,等.痰热清注射液治疗流感病毒A型上呼吸道感染临床观察[J].中国中医急症,2005,14(1)：26.
[100]杨钦河,唐雪春,陈孝银,等.咳宁方对流感病毒性肺炎小鼠肺组织HSP70表达的影响[J].中国病理生理杂志,2004，20(6)：1062.
[101]张晓梅.柴胡桂枝汤加减方对上呼吸道病毒感染者IL-6、TNF-β的调节作用[J].北京中医药大学学报,2002,25(1)：60.
[102]李文,王少军,严敏,等.鱼腥草提取液抗流感、腮腺炎病毒效果观察[J].预防医学文献信息,1999,5(4)：347,348.
[103]郭惠,姚桃,何士勤.鱼腥草抗流感病毒诱导细胞凋亡的研究[J].赣南医学院学报,2003,23(6)：615,616.
[104]石钺,石任兵.我国药用金银花资源,化学成分及药理研究进展[J].中国药学杂志,1999,34(11)：724-727.
[105]王志杰.鱼腥草抗病毒实验研究[J].预防医学文献,1999,5(4)：43-47.
[106]李颖,刘宏,叶北.板蓝根提取物抗流感病毒FM1作用[J].中国医疗前沿,2008,4,3(8)：32-34.
[107]张振亚,方学平,刁志花,等.野菊花提取物抑制呼吸道合胞病毒作用的体外实验研究[J].解放军药学学报,2002,22(4)：273,274.
[108]王意忠,崔晓兰,高英杰,等.栀子提取物抗病毒试验[J].中国中药杂志,2006,31(14)：1176-1178.
[109]张凤梅,李洪源,李霞,等.败酱草多糖体外抗呼吸道合胞病毒作用的研究[J].黑龙江医药科学,2006，29(1)：48-50.
[110]左丽,陈丽,唐小敏,等.黄芪A6组分对流感病毒感染小鼠的防治作用[J].中国病毒学,1997,12(4)：342-345.
[111]赵文,任永风,樊建航,等.新疆黄芪抗病毒作用研究[J].中国药学杂志,2001,36(1)：23-25.
[112]蒋成全.中药抗病毒作用及其应用[J].中国医药导报,2007,4(15)：8,9.
[113]毛淑才,刘展眉,郑康成.贯叶连翘提取物的研究新进展[J].时珍国医国药,2006,17(7)：1303-1305.
[114]张海燕.连翘化学成分及药理活性的研究进展[J].中药材,2000,23(10)：657,658.
[115]王雪峰,周丽.柴胡黄芩提取物对CVB3m感染小鼠心肌损伤影响的实验研究[J].辽宁中医杂志,2004,31(11)：890,891.
[116]卫文峰,张国成,许东亮,等.柴胡黄芩炙甘草对小鼠CVB3心肌炎治疗作用的研究[J].中国当代儿科杂志,2003,5(3)：223-225.
[117]刘文辉,石军飞,吴晓忠.黄答提取物体外抗流感病毒的比较研究[J].内蒙古医学杂志,2010,42(1)：7-9.
[118]刘钊,杨占秋,肖红.中药大青叶有效单体抗流感病毒作用[J].中南民族大学学报(自然科学版),2009,9,28(3)：42-46.
[119]温云海,林岳生,黄海,等.金莲花水浸提取液抗病毒的实验研究[J].中华微生物学和免疫学杂志,1999,19(1)：21.
[120]苏连杰,田鹤,马英丽.金莲花醇提物体内抗病毒作用的实验研究[J].中草约,2007,38(7)：1062-1064.
[121]严银芳,陈晓,杨小青,等.石香薷挥发油抗流感病毒活性成分的初步研究[J].青岛大学医学院学报,2002,32(2)：155-157.
[122]Kobayashi M, Davis SM, Utsunomiya T,et al. Antiviral effect of gingyosan, a traditional Chinese herbal medicine, on influenza A2 virus infection in mice[J]. Am J Ch in Med,1999,27 (1):53-55.
[123]王道坤,常见中草药的有效成分及应用[J],兽药市场指南,2005,(6)：22,23.
[124]江勤,董六一,方明,等.黄蜀葵花总黄酮体外抗单纯疱疹病毒作用[J].安徽医药,2006,10(2)：93,94.
[125]梁英,韩鲁佳.黄芩中黄酮类化合物药理学作用研究进展[J].中国农业大学学报,2003,8(6)：9-14.
[126]林凤秋,冯顺卿,李药兰,等.金莲花抑菌抗病毒活性成分的初步研究[J].浙江大学学报(理化版),2004,31(4)：412-415.
[127]冯煦,王鸣,赵友谊,等.北柴胡茎叶总黄酮抗流感病毒的作用[J].植物资源与环境学报,2002,11(4)：15-18.
[128]张建军,李洪源,王吉锡,等.甘草活性成分抗呼吸道合胞病毒的作用[J].中国公共卫生,2007,23(6)：723,724.
[129]王彦芳,秦林,王焱,等.中药抗病毒作用研究进展[J].医学综述,2008,14(22)：3488-3490.
[130]吴莹,金叶智,于雪飞,等.黄芩主要成分体外抗甲型流感病毒作用的研究[J].北京中医药大学学报,2010,33(8)：541-544.
[131]高阳,董雪,康廷国,等.牛蒡苷元体外抗流感病毒活性[J].中草药,2002,33(8)：724,725.
[132]周艳萌,吴中明,向晓波.土贝母皂苷体外抗乙型肝炎病毒的药效研究[J].时珍国医国药,2006,17(11)：2134-2136.
[133]邹宇宏,杨雁,吴强,等.黄芪提取物的体外抗乙肝病毒作用[J].安徽医科大学学报,2003,38(4)：267-269.
[134]Oh KW, Lee CK, Kim YS,et al.Anti-herpetic activities of acidic protein bound polysaccharied isolated from Ganoderma lucidum alone and in combinations with acyclovir and vidarabine[J]. Jorunal of Ethnopharmacolgy,2000,72:221-227.
[135]Xu HX, Lee SHS, Lee SF,et al. Isolation and characterization of and anti HSV polysaccharide form Prunel Lavulgaris[J]. Antiviral Research,1999,44(1):43-54.
[136]刘晓玲,张勇,刘小雷.苦参系列生物碱体外抗CVB3病毒活性[J].沈阳药科大学学报,2006,23(11)：724-730.
[137]辜红梅.黄精多糖的抗单纯泡疹病毒作用[J].应用与环境生物学,2003,9(1)：21.
[138]黎敬波,刘叶.3种解表方法抗流感病毒甲1型作用的实验研究[J].新中医，2004,36(1)：78.
[139]Farnet CM, Wang B, HansenM,et al. Human immunodeficiency virus type-I DNA integration now aromatic hydroxylated inhibitors and studies of the inhibition mechanism[J]. Antimirtobial Agents homotherm, 1998,42(9):2245-2248.
[140]徐以珍,蒋建祥,刘学甄,等.“调免病毒”中药提取液体外抗病毒的实验研究[J].生物免疫学进展,2001,29(4)：31-33.
[141]Ling Yi, Zhengquan Li. Kehu Yuan,et al. Small molecules blocking the entry of severer acute respiratory syndrome coronavirus into host cells[J]. Journal of viorology,2004,(10):11334-11339.
[142]何立巍,李祥,陈健伟,等.板蓝根抗病毒有效成分研究进展[J].中医药信息,2005,22(5)：37-40.
[143]廖伟胜,余道文,董继华.柴胡注射液抑制呼吸道合胞病毒的研究[J].深圳中西医结合杂志,1999,9(2)：20,21.
[144]陈灏珠主编.实用内科学[M].北京：人民卫生出版社,2005.317.
[145]Dawood FS, Jain S, Finelli L,et al. Emergence of a novel swine-origin influenza A(H1N1)virus in humans[J]. New Engl J Med,2009,360(25):2605-2615.
[146]干梦久等主编.病毒感染发病和治疗机制[M].吉林：长春出版社,2001.77.
[147]Lei Shang, Zhangyi Qu, Lihua Sun,et al. Astragaloside IV inhibits adenovirus replication and apoptosis in A549 cells in vitro[J]. JPP,2011,63:688-694.
[148]T Feuchtinger, P Lang, R Handgretinger. Adenovirus infection after allogeneic stem cell transplantation [J]. Leukemia lymphoma,2007,48(2):244-255.
[149]Kevin L R, Anththony W, Hawksworth, et al. Vaccine-preventable adenoviral respiratory illness in US military recruits, 1999-2004[J]. Vac,2006,24(15):2835-2842.
[150]Nasreen B, Marye, Wroblewsk I. Adenovirus [J]. Pediatr Rev,2010,31(4):173,174.
[151]Baboonian C, Mekenna W. Eradication of viral myocarditis:is there hope?[J]. J AmColl Cardiol, 2003,3:473-476.
[152]Whitton JL. Immunopathology during coxsackievirus infection.[J]. Springer Semin Immunopathol, 2002,24:201-213.
[153]陈小夏,何冰,李显奇,等.广藿香三种提取物对肠道功能作用的比较[J].中药药理与临床,1998,14(2)：31-33.
[154]陈小夏,何冰,李显奇,等.广藿香胃肠道药理作用[J].中药材,1998,21(9)：465-467.
[155]杜一民,陈汝筑,胡本荣.广藿香的化学成分及其药理作用研究进展[J].中药新药与临床药理,1998,9(4)：238-241.
[156]刘尧,毛羽.广藿香挥发油止咳化痰药理实验的研究[J].时珍国医国药,2007,18(8)：1920,1921.
[157]罗超坤.广藿香水提物的抗菌实验研究[J].中药材,2005,28(8)：700,701.
[158]刘晓蓉,邓毛程,张媛媛.广藿香挥发油的提取及抑菌活性成分稳定性的研究[J].中国酿造,2009,8：87-90.
[159]刘晓蓉,范瑞,张媛媛,等.广藿香提取物的抑菌活性研究[J].食品科技,2009,34(5)：220-224,227.
[160]代敏,彭成,万峰,等.5味化湿药对奶牛乳腺炎病原菌体外抗菌活性的比较[J].江苏农业,2011,39(4)：240-244.
[161]苏镜娱,张广文,李核,等.广藿香精油化学成分分析与抗菌活性研究[J].中草药,2001,32(3)：204，205.
[162]杨得坡.藿香和广藿香挥发油对皮肤癣菌和条件致病真菌的抑制作用[J].中国药学杂志,2000,35(1)：10,11.
[163]罗集鹏,刘玉萍,冯毅凡,等.广藿香的两个化学别及产地与采收期对其挥发油成分的影响[J].药学学报,2003,(4)：307-310.
[164]魏刚,李颧,徐鸿华,等GC-MS建立在牌广守住香挥发油指纹图谱方法学研究[J1.中成药,2003,(2)：91-95.
[165]国家药典委员会.中国药典(I部)[s].北京：中国医药科技出版社,2010.42.
[166]张仲敏,黎玉翠,彭绍忠,等.广藿香醇研究概述[J].中国中医药信息杂志,2012,19(1)：110-112.
[167]Wu HX, Li BL, Wang X,et al.Inhibitory Effect and Possible Mechanism of Action of Patchouli Alcohol against Influenza A (H2N2) Virus [J]. Molecules,2011,16:6489-6501.
[168]Kiyohara H, Ichino C, Kawamura Y,et al. Patchouli alcohol:In vitro direct anti-influenza virus sesquiterpene in Pogostemon cablin Benth[J]. J Nat Med,2011,15:11480-11550.
[169]Li YC, Peng SZ, Chen HM,et al.Oral administration of patchouli alcohol isolated from Pogostemonis Herba augments protection against influenza viral infection in mice[J]. International Immunopharmacology, 2012,12:294-301.
[170]杨钤熹,谢培山.中药广藿香抗真菌成分-广藿香酮的分离及结构测定[J].科学通报,1977,(7)：318.
[171]曾庆钱,严振,蔡岳文,等.广藿香精油对檀香粉蝶和荔枝卷叶蛾生物活性研究[J].天然产物研究与开发,2006,18：541-544.
[172]曾庆钱,蔡岳文,严振,等.广藿香精油的杀虫作用及其活性成分分析[J].植物资源与环境学报,2006,15(3)：21-25.
[173]孔庚星.广藿香酮的分离及其在药剂中的应用[J].中国医院药学杂志,1986,(1)：32,33.
[174]Rezkalla S, Kloner RA, Khatib G,et al. Beneficial effects of captopril in acute coxsackievirus B3 murine myocarditis[J]. Circulation,1990,81(3):1039-1046.
[175]Su M, Li Y, Leung KT,et al. Antiviral activity and constituent of Ardisia chinensis benth against Coxsackie B3 virus[J]. Phytother Res,2006,20(8):634-639.
[176]Baboonian C, Mckenna W. Eradication of viral myocarditis:is there hope?[J]. J AmColl Cardiol, 2003,3:473-476.
[177]Mason JW. Myocarditis and dilated cardiomyopathy:an inflammatory link[J]. Cardiovas Res, 2003,60:5-10.
[178]Daubeney PE, Nugent AW, Chondros P,et al. Clinical features and outcomes of childhood dilated cardiomyopathy:results from a national population-based study[J]. Circulation,2006:114(24):2671-2678.
[179]杨英珍.病毒性心脏病[M].上海：上海科学技术出版社,2001.
[180]张凤英,段一娜,米树斌.病毒性心肌炎细胞凋亡及机制研究[J].承德医学院学报,2006,23(2)：179,180.
[181]王永祥,陆春雨,金世香等.柯萨奇病毒B3感染致小鼠心肌细胞凋亡的研究[J].河北医科大学学报,2003,24(5)：257-260.
[182]Joo CH, Hong HN, Kim EO. Cardiomyocyte apoptosis experimental coxsackie virus B3 myocarditis[J]. Intervirology,2003,46(3):135-140.
[183]张松等TNF-α mRNA在小鼠VMC中表达及意义[J].中华病理学杂志,2002,6(31)：250-252.
[184]Vada H, Saitok, Kanda T,et al. Tumor necrosis factor-alpha(TNF-α) plays a protective role in acute viral myocarditis in mice. A study using mice Lacking TNF-α[J]. Circulation,2001,130:743-749.
[185]林国珍,朱霖,施咏梅.病毒性心肌炎患儿抗氧化能力与营养素含量测定[J].微量元素与健康研究,2001,18(2)：29,30.
[1]徐国钧,王强.中草药彩色图谱[M].福州：福建科学技术出版社,2006.
[2]中国医学科学院药物研究所.中药志[M].北京：人民卫生出版社,1988.128.
[3]吴友根,郭巧生,郑焕强.广藿香本草及引种历史考证的研究[J].中国中药杂志,2007,32(20)：2114-2117,2181.
[4]国家药典委员会.中华人民共和国药典2010年版：一部[M].北京：中国医药科技出版社,2010.42,43.
[5]吴贻谷.中国医学百科全书(中药学)[M].上海：上海科学技术出版社,1991.166,167.
[6]叶卫玲.广藿香与藿香的鉴别[J].海峡药学,2011,23(11)：43-45.
[7]年忠梅,丛浦珠.藿香的研究进展[J].湖北中医杂志,1990,1：44-47.
[8]唐慎微.重修政和经史证类备用本草[M].北京：人民卫生出版社,1957.309.
[9]陶弘景.名医别录(辑校本)[M].尚志钧辑校.北京：人民卫生出版社,1986.64.
[10]任守忠,靳德军,张俊清,等.广藿香药理研究进展[J].中国现代中药,2006,8(8)：27.
[11]贾旭玲,徐三,施芬.广藿香的研究进展[J].中华中医药学刊,2010,11(28)：2442-2444.
[12]梅全喜,张永流.藿香正气丸的临床新用途[J].中药材,2002，25(11)：848.
[131吴周和,刘建成,吴小刚.藿香中天然防腐剂的提取方法及其抑菌作用研究[J].中国调味品,2004(8)：18-20.
[14]莫小路,严振,王玉生,等.广藿香精油对植物病原真菌的抑菌活性研究[J].中药材,2004,27(11)：805-807.
[15]刘亮锋,黄晓丹,蔡大可,等.广藿香油及藿香油的研究概况[J].中国中医药信息杂志,2009,16(2)：100-102.
[16]陈小夏,何冰,李显奇,等.广藿香三种提取物对肠道功能作用的比较[J].中药药理与临床,1998,14(2)：31-33.
[17]何冰,陈小夏,罗集鹏,等.广藿香去油5部分不同极性提取物对胃肠道的影响[J].中药材,2001,24(6)：422.
[18]朱金照,冷恩仁,桂先勇,等.白术、藿香等中药对胃排空、肠推进影响的实验研究[J].中国中医基础医学杂志,2000,6(1)：21-23.
[19]朱金照,张捷,张志坚.藿香提取液对大鼠小肠一氧化氮合酶分布的影响[J].福建医药杂志,2002,24(3)：99-100.
[20]陆茵,陈文星,孟政杰,等.加味藿香正气软胶囊调节胃肠运动功能[J].中药新药与临床药理,2003,14(6)：381-383.
[21]杨国汉,胡德耀,戴裕光,等.藿香正气液及其组方药物对大鼠胃排空和肠推进作用的影响[J].实用中医药杂志,2005,21(9)：521-523.
[22]谢肆聪,唐方.藿香对肢体缺血-再灌注模型大鼠肠屏障功能保护作用的机理研究[J].中国中药杂志,2004,29(5)：61-63.
[23]刘琥琥,罗集鹏,赖沛炼.广东高要与吴川产广藿香提取物对肠道致病抗菌作用的比较研究[J].中药材,1999,22(8)：408-410.
[24]罗超坤.广藿香水提物的抗菌实验研究[J].中药材,2005,28(8)：700-701.
[25]苏镜娱,张广文,李核,等.广藿香精油化学成分分析与抗菌活性研究[J].中草药,2001,32(3)：204-205.
[26]杨得坡.藿香和广藿香挥发油对皮肤癣菌和条件致病真菌的抑制作用[J].中国药学杂志,2000,35(1)：10-11.
[27]孔祥麟译.从藿香中提取抗病毒成份[J].日本医学介绍,1983.4(5).
[28]刘世增,杨吉成,顾振纶.香菊感冒冲剂的药理作用研究[J],中成药,1997，19(3)：25.
[29]彭绍忠,李耿,秦臻,等.广藿香不同提取部位体内抗流感病毒作用研究[J1.时珍国医国药,2011,22(11)：2578-2579.
[30]高相雷,熊盛,王一飞,等.广藿香三种有效部位体外抗柯萨奇病毒B3作用的初步研究[J].中成药,2009,32(5)：261-264.
[31]贺玉琢.藿香镇吐活性成分的研究[J].国外医学·中医中药分册,1998,20(5)：61.
[32]刘中煜,袁美娟,聂正慧,等.藿香正气水解痉、镇痛和抗菌作用实验观察[J].中草药,1984,15(12)：543.
[33]冯承浩,隋春花,吴鸿.广藿香挥发油的合成场所及储存部位研究[J].中草药,2007,38(1)：116-119.
[34]刘尧,毛羽.广藿香挥发油止咳化痰药理实验的研究[J].时针国医国药,2007,18(8)：1920,1921.
[35]刘晓蓉,邓毛程,张媛媛,广藿香挥发油的提取及抑菌活性成分稳定性的研究[J].中国酿造,2009,8：87-90.
[36]刘晓蓉,范瑞,张媛媛,等.广藿香提取物的抑茵活性研究[J].食品科技,2009,34(5)：220-224,227.
[37]代敏,彭成,万峰,等.5味化湿药对奶牛乳腺炎病原菌体外抗菌活性的比较[J].江苏农业,2011,39(4)：240-244.
[38]Xu HX, Lee SHS, Lee SF, et al. Isolation and characterization of and anti HSV polysaccharide form Brunel La vulgaris[J]. Antiviral Research,1999,44(1):43-54.
[39]张强,李章万,朱江粤.广藿香挥发油成分的分析[J].华西药学染志,1996,(4)：249，50.
[40]王俊华,符红.广藿香挥发油化分气质联用技术分析[J].时珍国医国药,2000,(7)：579,580.
[41]罗集鹏,刘玉萍,冯毅凡,等.广藿香的两个化学别及产地与采收期对其挥发油成分的影响[J].药学学报,2003,(4)：307-310.
[42]魏刚,李颧,徐鸿华,等.GC-MS建立在石牌广藿香挥发油指纹图谱方法学研究[J].中成药,2003,(2)：91-95.
[43]肖培根.新编中药志(第三卷)[M].北京：化学工业出版社,2001.16.
[44]张仲敏,黎玉翠,彭绍忠,等.广藿香醇研究概述[J].中国中医药信息杂志,2012,19(1)：110-112.
[45]罗集鹏,冯毅凡,郭晓玲.不同采收期对广藿香产量及挥发油成分的影响[J].中药材, 2001,24(5)：316,317.
[46]Bhatia SP, Letizia CS, Api AM. Fragrance material review on patchouli alcohol[J]. Food and Chemical Toxicology,2008,46:255,256.
[47]张仲敏,黎玉翠,彭绍忠,等.广藿香醇研究概述[J].中国中医药信息杂志,2012,19(1)：110-112.
[48]Yanfang Xian, Yucui Li, Siu popl. Anti-inflammatory effect of patchouli alcohol isolated from Pogostemonis Herba in LPS-stimulated RAW264.7 macrophages[J]. Experimental and Therapeutic Medicine, 2011,2:545.
[49]Ngampong Kongkathip, Pornpat Samang, Boonsong Kongkathip, et al. Development of Patchouli Extraction with Quality Control and Isolation of Active Compounds with Antibacterial Activity[J]. Kasetsart J, 2009,43:519.
[50]Betty C, R Zhu, Gregg Henderson, et al. Toxicity and Repellency of Patchouli Oil and Patchouli Alcohol against Formosan Subterranean Termites Coptotermes formosanus Shiraki[J]. J. Agric. Food Chem, 2003,51:458-465.
[51]黄晓舞,白林,徐风华,等.广藿香醇对β-淀粉样蛋白神经毒性的抑制作用[J].解放军药学学报,2008,24(4)：338.
[52]黄晓舞,栾复新,徐风华.百秋李醇在制备防治老年性痴呆症的药物中的用途：中国专利.200710176344.8[P].2008-06-11.
[53]Mirko W, Anja S, Bockmuehl AB, et al. Agents agains microorganisms containing patchouli oil, patchouli alcohol and/or the derivatives[P]. United States:0134239 A1,2006-6-22.
[54]王洋.广藿香中新的具抗锥虫活性的倍半萜氢过氧化物[J].国外医学：中医中药分册,2005,27(6)：358-358.
[55]Huaxing Wu, Beili Li, Xue Wang, et al. Inhibitory Effect and Possible Mechanism of Action of Patchouli Alcohol against Influenza A (H2N2) Virus [J]. Molecules,2011,16,6489-6501.
[56]Kiyohara H, Ichino C, Kawamura Y, et al. Patchouli alcohol:In vitro direct anti-influenza virus sesquiterpene in Pogostemon cablin Benth[J]. J.Nat.Med,2011,15:652-657.
[57]Yucui Li, Shaozhong Peng, Haiming Chen, et al. Oral administration of patchouli alcohol isolated from Pogostemonis Herba augments protection against influenza viral infection in mice[J]. International Immunopharmacology,2012(12):294-301.
[58]任守忠,张俊清,刘明生.广藿香酵的急性毒性实验[J].海南医学院学报,2009,15(12)：1503-1504.
[59]杨甫传,徐丽珍,邹忠梅,等.广藿香醇及广藿香油中广藿香醇在大鼠体内药代动力学比较[J].药学学报,2004,39(9)：726-729.
[60]Luu B, Guy Ourisson. Hydroxylation of patchoulenol by rabbits, hemisynthesis of nor-patchoulenol. The odour carrier of patchouli oil[J]. Tetrahedron letter,1975,26:2211-2214.
[61]孔庚星.广藿香酮的分离及其在药剂中的应用[J].中国医院药学杂志,1986,6(1)：32,33.
[62]E. Klein, W. Rojahn. Isolating structure and synthesis VON DHELWANGIN[J]. Tetrahedron Letters, 1969,(27):2279-2280.
[63]杨钤熹,谢培山.中药广藿香抗真菌成分广藿香酮的分离及结构测定[J].科学通报,1977,(7)：318.
[64]杜一民,陈汝筑,胡本荣.广藿香的化学成分及其药理研究进展[J].中国新药与临床药理,1998,9(4)：23-25.
[65]曾庆钱,严振,蔡岳文,等.广藿香精油对檀香粉蝶和荔枝卷叶蛾生物活性研究[J].天然产物研究与开发,2006,18：541-544.
[66]曾庆钱,蔡岳文,严振,等.广藿香精油的杀虫作用及其活性成分分析[J].植物资源与环境学报,2006,15(3)：21-25.
[2]李莹.中药抗呼吸道病毒感染药理作用研究进展[J].山西中医,2007,23(4)：71-72.
[3]李燕宁,吴金勇,周明,等.中成药抗呼吸道病毒感染的实验研究进展[J].山东医药,2011,15(51)：111-112.
[4]金奇.医学分子病毒学[M].北京：科学出版社,2011.
[5]Dawood FS, Jain S, Finelli L,et al. Emergence of a novel swin-origin influenza A (H IN 1) virus in humans[J]. New Engl J Med,2009,360(25):2605-2615.
[6]雷达,艾立新,邓红樱,等.流感大流行特征启示甲型H1N1流感防控策略[J].中国国境卫生检疫杂志,2009,32(3)：145-150.
[7]Salom D, Hill BR, Lear JD,et al. pH-dependent tetramerization and amantadine binding of the transmembrane helix of M2 from the influenza A Virus [J]. Biochemistry,2000,39(46):14160-14170.
[8]Jefferson T, Deeks JJ, Demicheli V,et al. Amantadine and rimantadine for preventing and treating influenza A in adults[J]. Cochrane Database Syst Rev,2004,(3):1169.
[9]Saito R, Sakai T, Sato I,et al. Frequency of amantadine-resistant influenza A viruses during two seasons featuring co-circulation of H1N1 and H3N2[J]. Journal of Clinical Microbiology,2003,41(5):2164-2165.
[10]Astrahan P, Kass I, Cooper MA,et al. A novel method of resistance for influenza against a channel blocking antiviral drug[J]. Proteins,2004,55(2):251-257.
[11]Gubareva LV. Molecular mechanisms of influenza virus resistance to neuraminidase Inhibitors[J]. Virus Research,2004,103(2):199-203.
[12]Matrosovich M, Klenk HD. Natural and synthetic sialic acid-containing inhibitors of influenza virus receptor binding[J]. Rev Med Virol,2003,13(2):85-97.
[13]Haynes LM, Jones LP, Barskey A,et al. Enhanced disease and pulmonary eosinophilia associated with formalin-inactivated respiratory syncytial virus vaccination are linked to G glycoprotein CX3C-CX3CR1 interaction and expression of substance P[J]. J Virol,2003,77(18):9831-9844.
[14]耿学辉,朱汝男,孙晓鸥,等.我国部分地区呼吸道台胞病毒分离株的亚型鉴定[J].中华儿科杂志,1997,35(7)：405-406.
[15]杨占秋,余宏.临床病毒学[M].北京：中国医药科技出版社,2000.103-105.
[16]Matsushima Y, Shimizu H, Kano A,et al. Novel human adenovirus strain, Bangladesh [J]. Emerg Infetc Dis,2012,18(5):846-848.
[17]谭耀驹,谢菲.腺病毒分型诊断研究进展[J].广东医学,2008,29(1)：156-157.
[18]曾军.流感样疾病的诊断和鉴别诊断[J].中华医学信息导报,2003,18(22)：15.
[19]van Tol MJ, Claas EC, Heemskerk B,et al. Adenovirus infection in children after allogeneic stem cell transplantation:diagnosis, treatment and immunity[J]. Bone Marrow Transplant,2005,35(1):573-576.
[20]陆权.儿科常见病毒性呼吸道感染的病因治疗[J].世界临床药物,2003,24(7)：398-403.
[21]Lei Shang, Zhangyi Qu, Lihua Sun,et al. Astragaloside IV inhibits adenovirus replication and apoptosis in A549 cells in vitro.J Pharm Pharmacol,2011,63:688-694.
[22]Nevels M, Tauber B, Spruss T, et al. "Hit-and-Run" transformation by adenovirus oncogenes [J]. J Virol, 2001,75(7):3089-3094.
[23]Lenaerts L, Naesens L. Antiviral therapy for adenovirus infections[J].Antiviral Res, 2006,71(2-3):172-180.
[24]杨英珍,金佩英,郭棋,等.实验性小鼠急性Coxsackie B病毒性心肌炎的研究[J].病毒学杂志,1989,3：233-238.
[25]Andreoletti L, Hober D, Becquart P,et al. Experimental CVB3-induced chronic myocarditis in two murine strains:evidence of interrelationships between virus replication and myocardial damage in persistent cardiac infection[J]. J Med Virol,1997,52(2):206-214.
[26]陈鸿珊.抗病毒药物及其研究方法[M].北京：化学工业出版社,2006：35.
[27]Matsumori A. Lessons from animal experiments in myocarditis. Herz,1992,17(2):107-111.
[28]郝静,王一飞,张美英,等.地不容粗提物体外抗柯萨奇B-3病毒活性作用的实验研究[J].汀苏中医药,2008,(9)：83-84.
[29]Fohlman J, Paukson K, Morein B,et al. High yield production of an inactivated Coxsackie B3 adjuvant vaccine with protective effect against experimental myocarditis[J]. Scand J Infec Dis Suppl,1993,88:103-108.
[30]Frazer JK, Jackson DG, Gaillard JP,et al Identification of centerin:a novel human germinal center B cell restricted serpin[J]. Eur J Immunol,2000,30(10):3039-3048.
[31]王志玉,王桂亭,许洪芝,等.大黄醇提液的抗疱疹病毒的作用[J].中国中药杂志,1996,21(6)：364.
[32]解光艳,程惠娟.中药抗单纯疱疹病毒实验研究进展[J].安徽中医学院学报,2011,30(3)：78-80.
[33]汪群水,周雪群.五种中药制剂体外抗呼吸道病毒效果及细胞毒性的研究[J].全科医学临床与教育,2009,7(3)：226-228.
[34]Wyde PR, Moore-Poveda DK, O'Hara B,et al. CL387626 exhibits marked and unusual antiviral activity against respiratory syncytial virus in tissue culture and in cotton rats. Antiviral Res,1998,38:31-42.
[35]Lewinsohn DM, Bowden RA, Mattson D,et al. Phase I study of intravenous ribavirin treatment of respiratory syncytial virus pneumonia after marrow transplantation[J]. Antimicrob Agents Chemother,1996,40(11):2555-2557.
[36]汪永江,俞建,时梳民等.中药退热微型灌剂抗病毒作用研究[J].上海中医药杂志,2001,7(3)：41-42.
[37]Moler FW, Steihnart CM, Ohmit SE,et al. Effectiveness of ribavirin in otherwise well infants with respiratory syncytial virus-associated respiratory failure[J]. J Pediatr,1996,128(3):422-428.
[38]汉·张仲景.仲景全书·伤寒论(第4版)[M].北京：中医古籍出版社.1997.
[39]宋·杨士赢.仁斋直指附遗方论[M].台北：台北新文丰出版公司.1982.
[40]宋·陈师文,等.太平惠民和剂局方[M].北京：人民卫生出版社.1959.
[41]元·朱丹溪.丹溪心法[M].上海：上海科学技术出版社.1959.
[42]干振华,李金恒.中药抗病毒药理作用的研究进展[J].医学研究生学报,2007,20(12)：1290-1293.
[43]王玮,高永翔.中药抗病毒研究进展[J].现代中西医结合杂志,2008,7(17)：2753-2754.
[44]李英杰.中西医治疗流感高热疗效分析[J].现代中西医结合杂志,2001,10(3)：214.
[45]王英燕,王海.抗病毒中药的研究进展[J].中医药学报,2006,34(1)：25.
[46]喻凯,户上泰彦.392种植物提取成分的抗病毒活性研究[J].华西药学杂志,2004,19(3)：186-1 88.
[47]Cowan MM. Plant products as antimicrobial agents[J]. Clin Microbiol Rev, 1999(10):564-582.
[48]郭洁文,杨振淮,潘竞锵,等.蛇葡萄属植物抗病毒、抑菌和抗炎镇痛作用的研究概况[J].中医研究,2004,17(5)：57-59.
[49]胡嵘,张辉,徐风英,等.抗病毒动物药研究进展[J].吉林中医药,2003,12,23(12)：56-58.
[50]Bonn D. Green coffee beans may yield new class of anti-HIV-1 agents Lancet,1998,352(9133):1039-1042.
[51]Tagaya E, Tamaoki J, Kawatani K,et al. Inhibition of airway smooth muscle tone by Chinese herbal medicines[J]. Euro Respir J,2000,16(6):1123-1128.
[52]王海颖.中药与免疫的研究概况[J].中医研究,2000,13(5)：41-43.
[53]梁丽,徐磁海.中草药抗炎抗菌抗病毒成分的研究[J].长春中医学院学报,1996,15(2)：47-48.
[54]徐国钧,王强.中草药彩色图谱[M].福州：福建科学技术出版社,2006.
[55]中国医学科学院药物研究所.中药志(第4册)[M].北京：人民卫生出版社,1988.128.
[56]吴友根,郭巧生,郑焕强.广藿香本草及引种历史考证的研究[J].中国中药杂志,2007,32(20)：2114-2117,2181.
[57]国家药典委员会.中华人民共和国药典(一部)[S].北京：化学工业出版社,2003.3.
[58]年忠梅,丛浦珠.藿香的研究进展[J].湖北中医杂志,1990,1：44-47.
[59]唐慎微.重修政和经史证类备用本草[M].北京：人民卫生出版社,1957.309.
[60]陶弘景.名医别录(辑校本)[M].尚志钧辑校.北京：人民卫生出版社,1986.64.
[61]任守忠,靳德军,张俊清,等.广藿香药理研究进展[J].中国现代中药,2006,8(8)：27.
[62]鲁建江,王莉,陈宏伟,等.微波法提取藿香中的挥发油[J].ITCM,2001,18(5)：40.
[63]李建春,傅莺,孙玉明.广藿香挥发油β-环糊精包合工艺的研究[J].湖北中医杂志,2001,23(4)：52.
[64]薛漓.广藿香及其混淆品广防风的鉴别[J].中草药,2001,32(5)：460,461.
[65]肖省娥,贺红,徐鸿华,等.广藿香组织培养与植株再生研究[J].中药材,2001,24(6)：391,392.
[66]张广文,马祥全,苏镜娱,等.广藿香中的黄酮类化合物[J].中草药,2001,32(10),：871-874
[67]罗集鹏,冯毅凡,郭晓玲,等.不同采收期对广藿香产量及挥发油成分的影响[J].中药材,2001,24(5)：316,317.
[68]何冰,陈小夏,罗集鹏,等.广藿香去油部分的5钟不同极性提取物对胃肠道的影响[J].中药材,2001,24(6)：422,423.
[69]刘卓妍,徐法,张进,等.气相色谱法测定小儿广朴止泻口服液中广藿香醇含量[J].中国医院药学杂志,2001：21(11)：672,673.
[70]李立忠,胡朝凤,全载哲.藿香中广藿香酮的分离及鉴定[J].中南民族大学学报,2005,24(1)：14-16.
[71]吴周和,刘建成,吴小刚.藿香中天然防腐剂的提取方法及其抑菌作用研究[J].中国调味品,2004(8)：18-20.
[72]莫小路,严振,王玉生,等.广藿香精油对植物病原真菌的抑菌活性研究[J].中药材,2004,27(11)：805-807.
[73]刘亮锋,黄晓丹,蔡大可,等.广藿香油及藿香油的研究概况[J].中国中医药信息杂志,2009,16(2)：100-102.
[74]刘世增,杨吉成,顾振纶.香菊感冒冲剂的药理作用研究[J].中成药,1997,19(3)：25.
[75]高相雷,熊盛,王一飞,等.广藿香三种有效部位体外抗柯萨奇病毒B3作用的初步研究[J].中成药,2009,32(5)：261-264.
[76]贾旭玲,徐三,施芬.广藿香的研究进展[J].中华中医药学刊,2010,11(28)：2442-2444.
[77]肖培根.新编中药志(第三卷)[M].北京：化学工业出版社,2001.16.
[78]国家药典委员会.中国药典(Ⅰ部)[S].北京：中国医药科技出版社,2010：42.
[79]Xian YF, Li YC, Ip SP,et al. Anti-inflammatory effect of patchouli alcohol isolated from Pogostemonis Herba in LPS-stimulated RAW264.7 macrophages[J]. Exp Ther Med,2011,2:545-555.
[80]Kongkathip N, Samang P, Kongkathip B,et al. Development of patchouli extraction with quality control and isolation of active compounds with antibacterial activity[J]. Kasetsart J.(Nat.Sci.) 2009,43:519.
[81]Betty CR, Gregg Henderson, Ying Yu,et al. Toxicity and repellency of patchouli oil and patchouli alcohol against formosan subterranean termites coptotermes formosanus shiraki[J]. J.Agric.Food Chem,2003,51:4585.
[82]黄晓舞,白林,徐风华,等.广藿香醇对β淀粉样蛋白神经毒性的抑制作用[J].解放军药学学报,2008,24(4)：338.
[83]杜一民.广藿香的化学成分及其药理研究进展[J].中国新药与临床药理,1998,9(4)：23-25.
[84]彭成,代敏,万峰.藿香油在制备治疗细菌感染性疾病的药物中的用途[专利]2011.5.18.
[85]Mosmann T. Rapid colorimetric assay for cellular growth and survival:application to proliferation and cytotoxicity assays[J]. Immunol Methods,1983,65:55-63.
[86]中国医学科学院流行病防治研究所编.常见病毒实验技术[M].北京：科学出版社,1978.34.
[87]中华人民共和国卫生部药政局.抗病毒药物药效指导原则[S].新药(西药)临床前研究指导原则汇编,1993.
[88]Madisch I, Harste G, Pommer H,et al. Phylogenetic analysis of the main neutralization and hemagglutination determinants of all human adenovirus prototypes as a basis for molecular classification and taxonomy[J]. J. Viro,2005,79(24):15265-15276.
[89]沈丕安.中医抗病毒感染的治法和体会[J].世界临床药物,2007,28(11)：646-650.
[90]黄家诏.试析外感病辨治中的动态思维方法[J].江苏中医药,2006,27(11)：11.
[91]张静,杨维中,郭元吉,等.中国2001-2003年流行性感冒流行特征分析[J].中华流行病学杂志,2004,25(6)：461.
[92]杨京华,许尤佳,阳荣秀,等.120例小儿病毒性肺炎常见病原体与中医证候的关系研究[J].四川中医,2006,24(3)：78
[93]欧爱华,罗翌,严夏,等.SARS与急性上呼吸道感染中医证候分型及指标数量化方法的探讨[J].中国卫生统计,2006,23(4)：309.
[94]张于华.从“因地制宜”看区域地理环境和“中医外感病”的关系[J].中华现代中西医杂志,2005,3(12)：1105.
[95]李梦东.实用传染病学第2版[M].北京：人民出版社1998.18.
[96]Yaolan Li,Linda S,M.Ooil,et al. Antiviral Activities of Medicinal Herbs Traditionally Used in Southern Mainland China[J].Phytother Res,2004,18:718-722.
[97]胡天佑.具有抗病毒作用的中草药应用研究[J].中国处方药,2004,8(29)：69,70.
[98]史利卿.抗呼吸道病毒中药的实验研究述评[J].中国中医基础医学杂志,1998,4(12)：54,55.
[99]曲敬来,高雪,周岁锋,等.痰热清注射液治疗流感病毒A型上呼吸道感染临床观察[J].中国中医急症,2005,14(1)：26.
[100]杨钦河,唐雪春,陈孝银,等.咳宁方对流感病毒性肺炎小鼠肺组织HSP70表达的影响[J].中国病理生理杂志,2004，20(6)：1062.
[101]张晓梅.柴胡桂枝汤加减方对上呼吸道病毒感染者IL-6、TNF-β的调节作用[J].北京中医药大学学报,2002,25(1)：60.
[102]李文,王少军,严敏,等.鱼腥草提取液抗流感、腮腺炎病毒效果观察[J].预防医学文献信息,1999,5(4)：347,348.
[103]郭惠,姚桃,何士勤.鱼腥草抗流感病毒诱导细胞凋亡的研究[J].赣南医学院学报,2003,23(6)：615,616.
[104]石钺,石任兵.我国药用金银花资源,化学成分及药理研究进展[J].中国药学杂志,1999,34(11)：724-727.
[105]王志杰.鱼腥草抗病毒实验研究[J].预防医学文献,1999,5(4)：43-47.
[106]李颖,刘宏,叶北.板蓝根提取物抗流感病毒FM1作用[J].中国医疗前沿,2008,4,3(8)：32-34.
[107]张振亚,方学平,刁志花,等.野菊花提取物抑制呼吸道合胞病毒作用的体外实验研究[J].解放军药学学报,2002,22(4)：273,274.
[108]王意忠,崔晓兰,高英杰,等.栀子提取物抗病毒试验[J].中国中药杂志,2006,31(14)：1176-1178.
[109]张凤梅,李洪源,李霞,等.败酱草多糖体外抗呼吸道合胞病毒作用的研究[J].黑龙江医药科学,2006，29(1)：48-50.
[110]左丽,陈丽,唐小敏,等.黄芪A6组分对流感病毒感染小鼠的防治作用[J].中国病毒学,1997,12(4)：342-345.
[111]赵文,任永风,樊建航,等.新疆黄芪抗病毒作用研究[J].中国药学杂志,2001,36(1)：23-25.
[112]蒋成全.中药抗病毒作用及其应用[J].中国医药导报,2007,4(15)：8,9.
[113]毛淑才,刘展眉,郑康成.贯叶连翘提取物的研究新进展[J].时珍国医国药,2006,17(7)：1303-1305.
[114]张海燕.连翘化学成分及药理活性的研究进展[J].中药材,2000,23(10)：657,658.
[115]王雪峰,周丽.柴胡黄芩提取物对CVB3m感染小鼠心肌损伤影响的实验研究[J].辽宁中医杂志,2004,31(11)：890,891.
[116]卫文峰,张国成,许东亮,等.柴胡黄芩炙甘草对小鼠CVB3心肌炎治疗作用的研究[J].中国当代儿科杂志,2003,5(3)：223-225.
[117]刘文辉,石军飞,吴晓忠.黄答提取物体外抗流感病毒的比较研究[J].内蒙古医学杂志,2010,42(1)：7-9.
[118]刘钊,杨占秋,肖红.中药大青叶有效单体抗流感病毒作用[J].中南民族大学学报(自然科学版),2009,9,28(3)：42-46.
[119]温云海,林岳生,黄海,等.金莲花水浸提取液抗病毒的实验研究[J].中华微生物学和免疫学杂志,1999,19(1)：21.
[120]苏连杰,田鹤,马英丽.金莲花醇提物体内抗病毒作用的实验研究[J].中草约,2007,38(7)：1062-1064.
[121]严银芳,陈晓,杨小青,等.石香薷挥发油抗流感病毒活性成分的初步研究[J].青岛大学医学院学报,2002,32(2)：155-157.
[122]Kobayashi M, Davis SM, Utsunomiya T,et al. Antiviral effect of gingyosan, a traditional Chinese herbal medicine, on influenza A2 virus infection in mice[J]. Am J Ch in Med,1999,27 (1):53-55.
[123]王道坤,常见中草药的有效成分及应用[J],兽药市场指南,2005,(6)：22,23.
[124]江勤,董六一,方明,等.黄蜀葵花总黄酮体外抗单纯疱疹病毒作用[J].安徽医药,2006,10(2)：93,94.
[125]梁英,韩鲁佳.黄芩中黄酮类化合物药理学作用研究进展[J].中国农业大学学报,2003,8(6)：9-14.
[126]林凤秋,冯顺卿,李药兰,等.金莲花抑菌抗病毒活性成分的初步研究[J].浙江大学学报(理化版),2004,31(4)：412-415.
[127]冯煦,王鸣,赵友谊,等.北柴胡茎叶总黄酮抗流感病毒的作用[J].植物资源与环境学报,2002,11(4)：15-18.
[128]张建军,李洪源,王吉锡,等.甘草活性成分抗呼吸道合胞病毒的作用[J].中国公共卫生,2007,23(6)：723,724.
[129]王彦芳,秦林,王焱,等.中药抗病毒作用研究进展[J].医学综述,2008,14(22)：3488-3490.
[130]吴莹,金叶智,于雪飞,等.黄芩主要成分体外抗甲型流感病毒作用的研究[J].北京中医药大学学报,2010,33(8)：541-544.
[131]高阳,董雪,康廷国,等.牛蒡苷元体外抗流感病毒活性[J].中草药,2002,33(8)：724,725.
[132]周艳萌,吴中明,向晓波.土贝母皂苷体外抗乙型肝炎病毒的药效研究[J].时珍国医国药,2006,17(11)：2134-2136.
[133]邹宇宏,杨雁,吴强,等.黄芪提取物的体外抗乙肝病毒作用[J].安徽医科大学学报,2003,38(4)：267-269.
[134]Oh KW, Lee CK, Kim YS,et al.Anti-herpetic activities of acidic protein bound polysaccharied isolated from Ganoderma lucidum alone and in combinations with acyclovir and vidarabine[J]. Jorunal of Ethnopharmacolgy,2000,72:221-227.
[135]Xu HX, Lee SHS, Lee SF,et al. Isolation and characterization of and anti HSV polysaccharide form Prunel Lavulgaris[J]. Antiviral Research,1999,44(1):43-54.
[136]刘晓玲,张勇,刘小雷.苦参系列生物碱体外抗CVB3病毒活性[J].沈阳药科大学学报,2006,23(11)：724-730.
[137]辜红梅.黄精多糖的抗单纯泡疹病毒作用[J].应用与环境生物学,2003,9(1)：21.
[138]黎敬波,刘叶.3种解表方法抗流感病毒甲1型作用的实验研究[J].新中医，2004,36(1)：78.
[139]Farnet CM, Wang B, HansenM,et al. Human immunodeficiency virus type-I DNA integration now aromatic hydroxylated inhibitors and studies of the inhibition mechanism[J]. Antimirtobial Agents homotherm, 1998,42(9):2245-2248.
[140]徐以珍,蒋建祥,刘学甄,等.“调免病毒”中药提取液体外抗病毒的实验研究[J].生物免疫学进展,2001,29(4)：31-33.
[141]Ling Yi, Zhengquan Li. Kehu Yuan,et al. Small molecules blocking the entry of severer acute respiratory syndrome coronavirus into host cells[J]. Journal of viorology,2004,(10):11334-11339.
[142]何立巍,李祥,陈健伟,等.板蓝根抗病毒有效成分研究进展[J].中医药信息,2005,22(5)：37-40.
[143]廖伟胜,余道文,董继华.柴胡注射液抑制呼吸道合胞病毒的研究[J].深圳中西医结合杂志,1999,9(2)：20,21.
[144]陈灏珠主编.实用内科学[M].北京：人民卫生出版社,2005.317.
[145]Dawood FS, Jain S, Finelli L,et al. Emergence of a novel swine-origin influenza A(H1N1)virus in humans[J]. New Engl J Med,2009,360(25):2605-2615.
[146]干梦久等主编.病毒感染发病和治疗机制[M].吉林：长春出版社,2001.77.
[147]Lei Shang, Zhangyi Qu, Lihua Sun,et al. Astragaloside IV inhibits adenovirus replication and apoptosis in A549 cells in vitro[J]. JPP,2011,63:688-694.
[148]T Feuchtinger, P Lang, R Handgretinger. Adenovirus infection after allogeneic stem cell transplantation [J]. Leukemia lymphoma,2007,48(2):244-255.
[149]Kevin L R, Anththony W, Hawksworth, et al. Vaccine-preventable adenoviral respiratory illness in US military recruits, 1999-2004[J]. Vac,2006,24(15):2835-2842.
[150]Nasreen B, Marye, Wroblewsk I. Adenovirus [J]. Pediatr Rev,2010,31(4):173,174.
[151]Baboonian C, Mekenna W. Eradication of viral myocarditis:is there hope?[J]. J AmColl Cardiol, 2003,3:473-476.
[152]Whitton JL. Immunopathology during coxsackievirus infection.[J]. Springer Semin Immunopathol, 2002,24:201-213.
[153]陈小夏,何冰,李显奇,等.广藿香三种提取物对肠道功能作用的比较[J].中药药理与临床,1998,14(2)：31-33.
[154]陈小夏,何冰,李显奇,等.广藿香胃肠道药理作用[J].中药材,1998,21(9)：465-467.
[155]杜一民,陈汝筑,胡本荣.广藿香的化学成分及其药理作用研究进展[J].中药新药与临床药理,1998,9(4)：238-241.
[156]刘尧,毛羽.广藿香挥发油止咳化痰药理实验的研究[J].时珍国医国药,2007,18(8)：1920,1921.
[157]罗超坤.广藿香水提物的抗菌实验研究[J].中药材,2005,28(8)：700,701.
[158]刘晓蓉,邓毛程,张媛媛.广藿香挥发油的提取及抑菌活性成分稳定性的研究[J].中国酿造,2009,8：87-90.
[159]刘晓蓉,范瑞,张媛媛,等.广藿香提取物的抑菌活性研究[J].食品科技,2009,34(5)：220-224,227.
[160]代敏,彭成,万峰,等.5味化湿药对奶牛乳腺炎病原菌体外抗菌活性的比较[J].江苏农业,2011,39(4)：240-244.
[161]苏镜娱,张广文,李核,等.广藿香精油化学成分分析与抗菌活性研究[J].中草药,2001,32(3)：204，205.
[162]杨得坡.藿香和广藿香挥发油对皮肤癣菌和条件致病真菌的抑制作用[J].中国药学杂志,2000,35(1)：10,11.
[163]罗集鹏,刘玉萍,冯毅凡,等.广藿香的两个化学别及产地与采收期对其挥发油成分的影响[J].药学学报,2003,(4)：307-310.
[164]魏刚,李颧,徐鸿华,等GC-MS建立在牌广守住香挥发油指纹图谱方法学研究[J1.中成药,2003,(2)：91-95.
[165]国家药典委员会.中国药典(I部)[s].北京：中国医药科技出版社,2010.42.
[166]张仲敏,黎玉翠,彭绍忠,等.广藿香醇研究概述[J].中国中医药信息杂志,2012,19(1)：110-112.
[167]Wu HX, Li BL, Wang X,et al.Inhibitory Effect and Possible Mechanism of Action of Patchouli Alcohol against Influenza A (H2N2) Virus [J]. Molecules,2011,16:6489-6501.
[168]Kiyohara H, Ichino C, Kawamura Y,et al. Patchouli alcohol:In vitro direct anti-influenza virus sesquiterpene in Pogostemon cablin Benth[J]. J Nat Med,2011,15:11480-11550.
[169]Li YC, Peng SZ, Chen HM,et al.Oral administration of patchouli alcohol isolated from Pogostemonis Herba augments protection against influenza viral infection in mice[J]. International Immunopharmacology, 2012,12:294-301.
[170]杨钤熹,谢培山.中药广藿香抗真菌成分-广藿香酮的分离及结构测定[J].科学通报,1977,(7)：318.
[171]曾庆钱,严振,蔡岳文,等.广藿香精油对檀香粉蝶和荔枝卷叶蛾生物活性研究[J].天然产物研究与开发,2006,18：541-544.
[172]曾庆钱,蔡岳文,严振,等.广藿香精油的杀虫作用及其活性成分分析[J].植物资源与环境学报,2006,15(3)：21-25.
[173]孔庚星.广藿香酮的分离及其在药剂中的应用[J].中国医院药学杂志,1986,(1)：32,33.
[174]Rezkalla S, Kloner RA, Khatib G,et al. Beneficial effects of captopril in acute coxsackievirus B3 murine myocarditis[J]. Circulation,1990,81(3):1039-1046.
[175]Su M, Li Y, Leung KT,et al. Antiviral activity and constituent of Ardisia chinensis benth against Coxsackie B3 virus[J]. Phytother Res,2006,20(8):634-639.
[176]Baboonian C, Mckenna W. Eradication of viral myocarditis:is there hope?[J]. J AmColl Cardiol, 2003,3:473-476.
[177]Mason JW. Myocarditis and dilated cardiomyopathy:an inflammatory link[J]. Cardiovas Res, 2003,60:5-10.
[178]Daubeney PE, Nugent AW, Chondros P,et al. Clinical features and outcomes of childhood dilated cardiomyopathy:results from a national population-based study[J]. Circulation,2006:114(24):2671-2678.
[179]杨英珍.病毒性心脏病[M].上海：上海科学技术出版社,2001.
[180]张凤英,段一娜,米树斌.病毒性心肌炎细胞凋亡及机制研究[J].承德医学院学报,2006,23(2)：179,180.
[181]王永祥,陆春雨,金世香等.柯萨奇病毒B3感染致小鼠心肌细胞凋亡的研究[J].河北医科大学学报,2003,24(5)：257-260.
[182]Joo CH, Hong HN, Kim EO. Cardiomyocyte apoptosis experimental coxsackie virus B3 myocarditis[J]. Intervirology,2003,46(3):135-140.
[183]张松等TNF-α mRNA在小鼠VMC中表达及意义[J].中华病理学杂志,2002,6(31)：250-252.
[184]Vada H, Saitok, Kanda T,et al. Tumor necrosis factor-alpha(TNF-α) plays a protective role in acute viral myocarditis in mice. A study using mice Lacking TNF-α[J]. Circulation,2001,130:743-749.
[185]林国珍,朱霖,施咏梅.病毒性心肌炎患儿抗氧化能力与营养素含量测定[J].微量元素与健康研究,2001,18(2)：29,30.
[1]徐国钧,王强.中草药彩色图谱[M].福州：福建科学技术出版社,2006.
[2]中国医学科学院药物研究所.中药志[M].北京：人民卫生出版社,1988.128.
[3]吴友根,郭巧生,郑焕强.广藿香本草及引种历史考证的研究[J].中国中药杂志,2007,32(20)：2114-2117,2181.
[4]国家药典委员会.中华人民共和国药典2010年版：一部[M].北京：中国医药科技出版社,2010.42,43.
[5]吴贻谷.中国医学百科全书(中药学)[M].上海：上海科学技术出版社,1991.166,167.
[6]叶卫玲.广藿香与藿香的鉴别[J].海峡药学,2011,23(11)：43-45.
[7]年忠梅,丛浦珠.藿香的研究进展[J].湖北中医杂志,1990,1：44-47.
[8]唐慎微.重修政和经史证类备用本草[M].北京：人民卫生出版社,1957.309.
[9]陶弘景.名医别录(辑校本)[M].尚志钧辑校.北京：人民卫生出版社,1986.64.
[10]任守忠,靳德军,张俊清,等.广藿香药理研究进展[J].中国现代中药,2006,8(8)：27.
[11]贾旭玲,徐三,施芬.广藿香的研究进展[J].中华中医药学刊,2010,11(28)：2442-2444.
[12]梅全喜,张永流.藿香正气丸的临床新用途[J].中药材,2002，25(11)：848.
[131吴周和,刘建成,吴小刚.藿香中天然防腐剂的提取方法及其抑菌作用研究[J].中国调味品,2004(8)：18-20.
[14]莫小路,严振,王玉生,等.广藿香精油对植物病原真菌的抑菌活性研究[J].中药材,2004,27(11)：805-807.
[15]刘亮锋,黄晓丹,蔡大可,等.广藿香油及藿香油的研究概况[J].中国中医药信息杂志,2009,16(2)：100-102.
[16]陈小夏,何冰,李显奇,等.广藿香三种提取物对肠道功能作用的比较[J].中药药理与临床,1998,14(2)：31-33.
[17]何冰,陈小夏,罗集鹏,等.广藿香去油5部分不同极性提取物对胃肠道的影响[J].中药材,2001,24(6)：422.
[18]朱金照,冷恩仁,桂先勇,等.白术、藿香等中药对胃排空、肠推进影响的实验研究[J].中国中医基础医学杂志,2000,6(1)：21-23.
[19]朱金照,张捷,张志坚.藿香提取液对大鼠小肠一氧化氮合酶分布的影响[J].福建医药杂志,2002,24(3)：99-100.
[20]陆茵,陈文星,孟政杰,等.加味藿香正气软胶囊调节胃肠运动功能[J].中药新药与临床药理,2003,14(6)：381-383.
[21]杨国汉,胡德耀,戴裕光,等.藿香正气液及其组方药物对大鼠胃排空和肠推进作用的影响[J].实用中医药杂志,2005,21(9)：521-523.
[22]谢肆聪,唐方.藿香对肢体缺血-再灌注模型大鼠肠屏障功能保护作用的机理研究[J].中国中药杂志,2004,29(5)：61-63.
[23]刘琥琥,罗集鹏,赖沛炼.广东高要与吴川产广藿香提取物对肠道致病抗菌作用的比较研究[J].中药材,1999,22(8)：408-410.
[24]罗超坤.广藿香水提物的抗菌实验研究[J].中药材,2005,28(8)：700-701.
[25]苏镜娱,张广文,李核,等.广藿香精油化学成分分析与抗菌活性研究[J].中草药,2001,32(3)：204-205.
[26]杨得坡.藿香和广藿香挥发油对皮肤癣菌和条件致病真菌的抑制作用[J].中国药学杂志,2000,35(1)：10-11.
[27]孔祥麟译.从藿香中提取抗病毒成份[J].日本医学介绍,1983.4(5).
[28]刘世增,杨吉成,顾振纶.香菊感冒冲剂的药理作用研究[J],中成药,1997，19(3)：25.
[29]彭绍忠,李耿,秦臻,等.广藿香不同提取部位体内抗流感病毒作用研究[J1.时珍国医国药,2011,22(11)：2578-2579.
[30]高相雷,熊盛,王一飞,等.广藿香三种有效部位体外抗柯萨奇病毒B3作用的初步研究[J].中成药,2009,32(5)：261-264.
[31]贺玉琢.藿香镇吐活性成分的研究[J].国外医学·中医中药分册,1998,20(5)：61.
[32]刘中煜,袁美娟,聂正慧,等.藿香正气水解痉、镇痛和抗菌作用实验观察[J].中草药,1984,15(12)：543.
[33]冯承浩,隋春花,吴鸿.广藿香挥发油的合成场所及储存部位研究[J].中草药,2007,38(1)：116-119.
[34]刘尧,毛羽.广藿香挥发油止咳化痰药理实验的研究[J].时针国医国药,2007,18(8)：1920,1921.
[35]刘晓蓉,邓毛程,张媛媛,广藿香挥发油的提取及抑菌活性成分稳定性的研究[J].中国酿造,2009,8：87-90.
[36]刘晓蓉,范瑞,张媛媛,等.广藿香提取物的抑茵活性研究[J].食品科技,2009,34(5)：220-224,227.
[37]代敏,彭成,万峰,等.5味化湿药对奶牛乳腺炎病原菌体外抗菌活性的比较[J].江苏农业,2011,39(4)：240-244.
[38]Xu HX, Lee SHS, Lee SF, et al. Isolation and characterization of and anti HSV polysaccharide form Brunel La vulgaris[J]. Antiviral Research,1999,44(1):43-54.
[39]张强,李章万,朱江粤.广藿香挥发油成分的分析[J].华西药学染志,1996,(4)：249，50.
[40]王俊华,符红.广藿香挥发油化分气质联用技术分析[J].时珍国医国药,2000,(7)：579,580.
[41]罗集鹏,刘玉萍,冯毅凡,等.广藿香的两个化学别及产地与采收期对其挥发油成分的影响[J].药学学报,2003,(4)：307-310.
[42]魏刚,李颧,徐鸿华,等.GC-MS建立在石牌广藿香挥发油指纹图谱方法学研究[J].中成药,2003,(2)：91-95.
[43]肖培根.新编中药志(第三卷)[M].北京：化学工业出版社,2001.16.
[44]张仲敏,黎玉翠,彭绍忠,等.广藿香醇研究概述[J].中国中医药信息杂志,2012,19(1)：110-112.
[45]罗集鹏,冯毅凡,郭晓玲.不同采收期对广藿香产量及挥发油成分的影响[J].中药材, 2001,24(5)：316,317.
[46]Bhatia SP, Letizia CS, Api AM. Fragrance material review on patchouli alcohol[J]. Food and Chemical Toxicology,2008,46:255,256.
[47]张仲敏,黎玉翠,彭绍忠,等.广藿香醇研究概述[J].中国中医药信息杂志,2012,19(1)：110-112.
[48]Yanfang Xian, Yucui Li, Siu popl. Anti-inflammatory effect of patchouli alcohol isolated from Pogostemonis Herba in LPS-stimulated RAW264.7 macrophages[J]. Experimental and Therapeutic Medicine, 2011,2:545.
[49]Ngampong Kongkathip, Pornpat Samang, Boonsong Kongkathip, et al. Development of Patchouli Extraction with Quality Control and Isolation of Active Compounds with Antibacterial Activity[J]. Kasetsart J, 2009,43:519.
[50]Betty C, R Zhu, Gregg Henderson, et al. Toxicity and Repellency of Patchouli Oil and Patchouli Alcohol against Formosan Subterranean Termites Coptotermes formosanus Shiraki[J]. J. Agric. Food Chem, 2003,51:458-465.
[51]黄晓舞,白林,徐风华,等.广藿香醇对β-淀粉样蛋白神经毒性的抑制作用[J].解放军药学学报,2008,24(4)：338.
[52]黄晓舞,栾复新,徐风华.百秋李醇在制备防治老年性痴呆症的药物中的用途：中国专利.200710176344.8[P].2008-06-11.
[53]Mirko W, Anja S, Bockmuehl AB, et al. Agents agains microorganisms containing patchouli oil, patchouli alcohol and/or the derivatives[P]. United States:0134239 A1,2006-6-22.
[54]王洋.广藿香中新的具抗锥虫活性的倍半萜氢过氧化物[J].国外医学：中医中药分册,2005,27(6)：358-358.
[55]Huaxing Wu, Beili Li, Xue Wang, et al. Inhibitory Effect and Possible Mechanism of Action of Patchouli Alcohol against Influenza A (H2N2) Virus [J]. Molecules,2011,16,6489-6501.
[56]Kiyohara H, Ichino C, Kawamura Y, et al. Patchouli alcohol:In vitro direct anti-influenza virus sesquiterpene in Pogostemon cablin Benth[J]. J.Nat.Med,2011,15:652-657.
[57]Yucui Li, Shaozhong Peng, Haiming Chen, et al. Oral administration of patchouli alcohol isolated from Pogostemonis Herba augments protection against influenza viral infection in mice[J]. International Immunopharmacology,2012(12):294-301.
[58]任守忠,张俊清,刘明生.广藿香酵的急性毒性实验[J].海南医学院学报,2009,15(12)：1503-1504.
[59]杨甫传,徐丽珍,邹忠梅,等.广藿香醇及广藿香油中广藿香醇在大鼠体内药代动力学比较[J].药学学报,2004,39(9)：726-729.
[60]Luu B, Guy Ourisson. Hydroxylation of patchoulenol by rabbits, hemisynthesis of nor-patchoulenol. The odour carrier of patchouli oil[J]. Tetrahedron letter,1975,26:2211-2214.
[61]孔庚星.广藿香酮的分离及其在药剂中的应用[J].中国医院药学杂志,1986,6(1)：32,33.
[62]E. Klein, W. Rojahn. Isolating structure and synthesis VON DHELWANGIN[J]. Tetrahedron Letters, 1969,(27):2279-2280.
[63]杨钤熹,谢培山.中药广藿香抗真菌成分广藿香酮的分离及结构测定[J].科学通报,1977,(7)：318.
[64]杜一民,陈汝筑,胡本荣.广藿香的化学成分及其药理研究进展[J].中国新药与临床药理,1998,9(4)：23-25.
[65]曾庆钱,严振,蔡岳文,等.广藿香精油对檀香粉蝶和荔枝卷叶蛾生物活性研究[J].天然产物研究与开发,2006,18：541-544.
[66]曾庆钱,蔡岳文,严振,等.广藿香精油的杀虫作用及其活性成分分析[J].植物资源与环境学报,2006,15(3)：21-25.
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