川渝地区鱼腥草资源化学成分研究
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摘要
鱼腥草药蔬兼用,根据《中华人民共和国药典》2005版,鱼腥草新鲜地上和地下部分以及干燥花期地上植株均可入药,而鱼腥草幼嫩茎叶则是鱼腥草主要产区人们餐桌上的一道特色蔬菜。其原植物为三白草科蕺菜属植物蕺菜Houttuynia cordata Thunb.。据《中国植物志》和《四川植物志》,蕺菜属植物仅蕺菜Houttuynia cordata Thunb.1种;2001年,祝正银和张士良在峨眉山发现蕺菜属一新种峨眉蕺菜Houttuynia emeiensis Z.Y.Zhuet S.L.Zhang。该种在峨眉、乐山等地俗称白侧耳根,也作蔬菜和鱼腥草药用。
四川和重庆地区的鱼腥草资源分布很广,遗传多样性较大。鱼腥草作为药材或蔬菜主要是利用其中的化学成分,而鱼腥草种质的不同会对这些化学成分产生影响。正确有效地评估鱼腥草资源各种化学成分间的差异,有助于更合理地开发利用这些资源。本研究对四川和重庆地区的鱼腥草资源挥发油成分、黄酮类成分、维生素成分的组成和含量以及部分重金属和农药的残留量进行了检测与分析,获得到如主要结果:
1.对18份新鲜鱼腥草材料地上和地下部分挥发油化学成分进行气相色谱质谱联用(GC/MS)分析发现,鱼腥草鲜草地上部分的挥发油成分中共鉴定出21种成分,材料间挥发油成分具有很大的遗传差异。首次发现根据鱼腥草地上部分挥发油成分可分为两种化学型:即癸醛型(D型)和月桂烯型(M型)。聚类分析和主成分分析结果表明,鱼腥草挥发油化学型与染色体数目有关,染色体数目少于80的所有材料均为D型,染色体数目大于或等于80的材料均为M型。D型鱼腥草的单萜类和倍半萜成分含量很低,平均仅5.72%和0.16%,其它非萜类直链化合物占整个挥发油组成的76.94%至94.71%。M型鱼腥草的单萜类和倍半萜含量较高,平均分别为36.16%和1.38%,其它直链化合物的浓度范围为37.88%～64.89%。试验结果还表明,鱼腥草挥发油化学型与其地理来源无关。鱼腥草地下部分挥发油中共鉴定出16种化合物,其不同材料地下部分挥发油成分组成及含量间差异均较小。本试验还发现,蕺菜和峨眉蕺菜间挥发油成分差异不显著。
2.采用RAPD、RAMP、ISSR和PCR-RFLP分子标记技术分析鱼腥草资源遗传多样性与挥发油化学型间关系。结果发现,在评价鱼腥草挥发油化学型与遗传背景多样性间关系上,ISSR分子标记较RAPD、RAMP和PCR-RFLP标记更有效,其遗传距离聚类分析结果与挥发油化学型分类结果几乎一致。但Mental test检验结果表明,基于本试验所用各种分子标记的遗传距离与挥发油成分的欧几里得距离间相关均未达显著水平。
3.通过对鱼腥草挥发油的紫外(UV)吸收光谱分析发现,鱼腥草挥发油在乙酸乙酯溶剂中的紫外吸收曲线的最大吸收波长λmax主要在252 nm和267 nm;23份试样的紫外吸收曲线可分为5种类型,化学型与吸收曲线类型不尽一致。各供试样品在两波长下的吸光度差异显著,23份试样的吸光度可分为3类。相关分析结果表明,挥发油成分中各主要萜类化合物含量与两个吸收峰的吸收值均呈正相关,且大多数达到极显著水平,而各主要不饱和烷烃类化合物含量与两个吸收峰的吸收值均呈显著或极显著的负相关。
4.分别使用不同浓度的D型和M型挥发油对赤霉菌(Fusarium graminearum)、炭疽菌(Colletotrichum coccodes)、灰霉菌(Botrytis cinerea)、大肠杆菌(Escherichia coli)和金黄色葡萄球菌(Staphylococcus aureus)的抑菌能力进行比较。结果表明,两种化学型挥发油的抑菌能力差异显著。D型挥发油对供试的各菌种,特别是对真菌的抑制能力明显高于M型挥发油。此外,同一挥发油对不同菌种抑制能力不同。
5.采用GC/MS法对生产中鱼腥草主栽品系的鲜草地上部分挥发油化学成分变化进行检测。方差分析结果表明,w01-100和w01-94的挥发油主要化学成分含量在一天中日照时间内无显著变化,以w01-94挥发油主要化学成分含量更稳定,w01-100和w01-94在整个白天内均可采收。此外,鱼腥草地上部分鲜草挥发油的部分主要成分间含量变化相关显著,但不同材料间相同两种成分间的相关性可能不同。
6.采用紫外-可见(UV-VIS)吸收光谱法和薄层色谱(TLC)法对提取挥发油后的剩余水溶液中黄酮类成分分析。显色鉴定和紫外-可见光谱扫描结果发现,提取剩余液中的黄酮类成分的种类丰富,主要类群有黄酮类、黄酮醇类、异黄酮类、二氢黄酮类和二氢黄酮醇类。薄层色谱结果表明这些成分中以苷类为主,含有芦丁和金丝桃苷成分。14份鱼腥草材料总黄酮含量差异达到极显著水平,平均含量为1.93 mg/g,以w01-16的总黄酮含量最高,为3.09 mg/g。鱼腥草资源的黄酮类成分种类及总黄酮含量与其染色体数目间均无显著相关。提取鱼腥草挥发油后的水溶液回收再利用价值明显。
7.采用高效液相色谱(HPLC)法比较鱼腥草资源间槲皮素含量差异。采用HypersilODS C18(250mm×4.0mm 5μm)色谱柱,流动相乙腈:0.4%磷酸=45:55,调pH=3,流速为1.0ml/min,柱温30℃,紫外检测波长370nm。结果发现,槲皮素含量在22.43 ng～2.24 ug范围内具有良好的线性关系,回归方程为Y=1.261458X-16.1218(r=0.9997),鱼腥草材料间槲皮素含量差异显著,w01-120中槲皮素含量水平最高,达4.02 mg/g。
8.采用HPLC法对鱼腥草幼嫩植株的维生素种类及含量进行测定,脂溶性维生素VA、δ-VE、VD_2、VD_3、α-VE和VK_1采用Hyperclone BDS C_(18)(150 mm×4.6 mm,5μm)色谱柱,以甲醇:乙酸乙酯(95:5)为流动相,275 nm下测定;水溶性维生素VB_1、VB_2、VB_6、烟酸和VC采用Luna C_(18)(2)100(?)(200 mm×4.6 mm,5μm)色谱柱,柱温30℃,以0.005 mol·mL~(-1)己烷磺酸钠(pH=3.2):甲醇(7:3),254 nm下测定。结果发现,鱼腥草幼嫩植株主要含有较多的α-VE和VC、含少量的VD_3、VK_1以及烟酸,而其余6种维生素均未检测到。鱼腥草资源间维生素种类差异很小,但含量差异显著。其地上部分维生素种类和含量比地下部分丰富,即鱼腥草地上部分维生素的营养价值高于其地下部分。
9.采用原子荧光(AFS)法同时测定不同鱼腥草资源中砷(As)和汞(Hg)含量。结果发现,在土壤中As的含量水平为11.557mg/kg,Hg的含量为0.387mg/kg的栽培条件下,鱼腥草幼嫩地上部分中As平均含量为0.161 mg/kg,Hg平均含量为0.004 mg/kg,幼嫩地下部分中两者的平均含量分别为0.130 mg/kg和0.0025 mg/kg。鱼腥草资源间对As、Hg积累的差异不显著,其As、Hg的含量低于国标规定限量。
10.采用气相色谱(GC)法,按GB/T14551-2003测定666和DDT的含量。鱼腥草幼嫩地上部分中仅小部分材料中检出痕量的δ-666。鱼腥草资源间对666、DDT积累的差异不显著,其666、DDT的含量低于国标规定限量。
综上所述,鱼腥草资源间在挥发油、黄酮类、维生素等自身代谢化学成分上差异显著;在重金属含量和农药残留量上差异不显著。其中,鲜草挥发油成分化学型的产生与其染色体数目变异高度相关,其他化学成分间差异与其染色体数目变异关系不明显。蕺菜和峨眉蕺菜在化学成分上并无显著差异。挥发油化学型的不同会导致其抗菌效果的不同,这对以鱼腥草挥发油为原料的药品生产和使用有重要意义。
HERBA HOUTTYNIAE,is a kind of traditional Chinese medicine and also a kind of delicious vegetable.According to Chinese Pharmacopoeia(2005),the fresh aerial and underground parts and the dry aerial parts during flowering period could be used as Chinese medicine.In the meantime,the fresh young stems and leaves are also delicious vegetable in its main spreading regions.HERBA HOUTTYNIAE came from Houttuynia cordata Thunb., which belongs to genus Houttuynia of Saururaceae.H.cordata is the only species in Houttuynia Thunb.according to Florae Reipublicae Popularis Sinicae and Flora Sichuanica. Zhu et al.(2001)classified a new species H,emeiensis Z.Y.Zhu et S.L.Zhang in the genus Houttuynia collected at Emei mountain in Sichuan province.It was called commonly as white Yuxingcao,which was also used as vegetable and HERBA HOUTTYNIAE in Emei and Leshan regions.
Houttuynia is widely spread in Sichuan and Chongqing regions of China.There existed plentiful genetic variations among genus Houttuynia germplasm resources.The chemical components of genus Houttuynia is the main reason why people utilized it as a kind of medicinal plants or vegetables.The diversities among Houttuynia germplasm resources would influence on these chemical components.Evaluation correctly and effectively on the variations among the chemical components of Houttuynia resources will help to the exploitation and ulilization of Houttuynia resources.In this paper,the compositions and their contents of the essential oils, flavones,vitamins and the rudimental contents of some heavy metals and pesticides of Houttuynia accessions in Sichuan and Chongqing regions were analyzed.The main results were as follows:
1.The essential oil(EO)compositions of 18 fresh Houttuynia accessions from Sichuan and Chongqing in China were analyzed by using GC/MS method.In total,21 components could be identified from the essential oils(EO)of the aerial parts of Houttuynia.High level of chemical polymorphism in the EOs of the aerial parts of Houttuynia was observed.On the basis of GC/MS data of the EOs of the aerial parts,two chemotypes could be identified for the frist time within the investigated Houttuynia accessions as follows:chemotypes decanal(D) and myrcene(M).The results of cluster analysis(CA)and principal component analysis(PCA) showed that the chemotypes of Houttuynia were correlated with the chromosome numbers. All the accessions classified into chemotype D have the chromosome number less than 80, while chemotype M had the chromosome number equal to or more than 80.The chemotype D showed lower amounts of monoterpenes and sequiterpenes,on average of 5.72%and 0.16%, respectively.The other non-terpenic straight chain components made up 76.94%to 94.71%of the whole EO components.In general,the chemotype M showed higher amounts of monoterpenes(average 36.16%)and sequiterpenes(1.38%),while the concentration of other straight chain components rang was from 37.88%to 64.89%.There was no significant relationship between the chemotypes and their geographical distributions.Totally,16 compounds were characterized from the essential oils(EO)of the underground parts of Houttuynia.There was no significant chemical difference between the EO of the underground parts of the different Houttuynia accessions.In this study,no significant chemical component difference was detected between the H.cordata and H.emeiensis accessions.
2.The relationships between the genetic diversity among Houttuynia germplasm resources and the essential oil chemotypes were analyzed by using RAPD,RAMP,ISSR and PCR-RFLP DNA markers technology.The results showed that the ISSR marker was more effective to evaluate the relationships than RAPD,RAMP and PCR-RFLP markers on the evualation of the relationships between the genetic diversity and the essential oil chemotypes.The cluster results of genetic distances(GDs)based on ISSR marker were almost coincident with the cluster results of euclidean distances based on the essential oils chemical components.However,the results of matrix correlation showed,there were insignificant correlation between EDs based on the essential oils chemical components and GDs based on any kind of DNA markers.
3.Through analyzing the UV spectrum of the essential oil of Houttuynia,it was found that theλmax of the essential oil of Houttuynia were at 252 nm and 267 nm in acetic ether solvent. Twenty-three samples could be divided into 5 types,which could not be coincident with the essential oil chemotypes.The differences of the absorbency of these samples at 252 nm and 267 nm were remarkable.Twenty-three samples counld be divided into 3 types based on the results of absorbency.All of the correlations between the contents of terpenes of EOs and the absorbencies of EOs at the two wavelengths were positive,and most of them reached 1% remarkable levlel.But all the correlations between the contents of unsaturated alkane of EOs and the absorbencies were negative,and all of them reached 5%or 1%remarkable levlel.
4.The abilities to restrain the growths of Fusarium graminearum,Colletotrichum coccodes, Botrytis cinerea,Escherichia coli and Staphylococcus aureus were compared by using the different concentrations of D chemotype and M type essential oils.The results showed that the difference of the restraining abilities of the two chemotypes was obvious.The restraining abilities of D chemotype essential oil were stronger than that of M chemotype essential oil, especially to restrain the growths of the epiphytes.And the same chemotype EO had different abilities to restraine the different microbial species.
5.The essential oil constituents of the fresh aerial parts of two lines were analyzed by GC/MS.The results of variance analysis showed that the essential oil of the two new lines w01-100 and w01-94 did not change significantly during daytime.The contents of the main components of the essential oil of w01-94 were more stable.Lines w01-100 and w01-94 can be harvested during the whole daytime.There were significant correlations between the content changes of some main components of the essential oil of the fresh aerial parts of Houttuynia,but the correlations between the two same components might be different in different materials.
6.The flavone components in the residuary water solution after essential oil distillation were analyzed by using UV-VIS spectrum and TLC.The results of displaying colors identifications and UV-VIS spectrum showed that there were abundance flavone components in the residuary water solution,mainly included flavone,flavonol,isoflavone, dihydroflavone and dihydroflavonol.The results of TLC showed that most of these flavone components in the residuary water solution were glycoside components,containing rutin and hyperin.The average content of total flavone of 14 Houttuynia accessions was 1.93 mg/g, the content of w01-16 was the highest,reaching to 3.09 mg/g.The difference of the content of total flavone among the 14 Houttuynia accessions was distinct.There were insignificant correlationships between the types of total flavone components,contents of total flavone and the chromosome numbers of Houttuynia accessions.The recycled values of the residuary water solution were obvious.
7.The difference of quercetin content of different Houttuynia accessions was determined by using HPLC on Hypersil ODS C18(250mm×4.0mm 5μm)at 30℃with acetonitrile:0.4% phosphoric acid(45:55),pH=3 as the mobile phase.Detection wavelength was at 370nm. The flow rate was 1.0mL/min.The calibration curve of quercetin was 22.43ng～2.24ug(r= 0.999 7).The quercetin content of the accession w01-120 was the highest,arriving at 4.02mg/g.
8.The vitamin constituents and content of fresh young Houttuynia accessions were determined by using HPLC methods.The fat-soluble vitamins VA,δ-VE,VD_2,VD_3,α-VE and VK_1 were determined by Hyperclone BDS C_(18)column(150mm×4.6mm 5u)at 25℃with methanol:ethyl acetate(95:5)as the mobile phase at 275nm;the water-soluble vitamins VB_1, VB_2,VB_6,Nicotinic acid and V_C were determined by Luna C_(18)(2)100(?)column(200 mm×4.6mm 5u)at 30℃with 0.005mol/L hexane sulfonic sodium(pH 3.2):methanol(70:30) as the mobile phase.The flow rate was at 1.0 mL/min.Detection wavelength was set at 254nm to determine the content of VB_1,VB_2,VB_6,Nicotinic acid and V_C of Hauttuynia.The mainly vitamin constituents wereα-VE and VC,less of VD_3、VK_1 and Nicotinic acid in Houttuynia,others 6 vitamins not detected in this research.The vitamin constituents and contents of the aerial parts were more abundant than that of underground parts in Houttuynia. The difference of the vitamin constituents among Houttuynia resource were little,but were notable in the content of their vitamins.The nutrition value of the aerial parts was higher than that of underground parts.
9.The remains of As and Hg in the young aerial and underground parts of 10 Houttuynia accessions and the soil cultivated these Houttuynia accessions were determined simultaneously by using AFS.The results showed that the content of As was 11.557mg/kg, Hg was 0.387mg/kg in soil,the mean content of As was 0.161mg/kg and Hg was 0.004mg/kg in the young aerial parts.And in the underground parts,the mean content of As was 0.130mg/kg and Hg was 0.0025mg/kg.The diference of the remains As and Hg among the Houttuynia accessions was insignificant.According to GB4810-94 and GB2762-94,the remains of heavy metals As and Hg were not beyond the standard.
10.The remains of 666 and DDT in the young aerial and underground parts of 10 Houttuynia accessions and the soil were determined simultaneously by using GC.The results showed that the remains ofδ-666 in soil was 0.17ng/kg,but the trace remains of the young aerial and underground parts in several accessions were detected.According to GB/T14551-2003,the remains of pesticides 666 and DDT were not beyond the standard.
To sum up,the differences of the self-metablism chemical components,such as the essential oils,flavones and vitamins were significant among the Houttuynia germplasm resources.And the variations on the rudimental contents of some heavy metals and pesticides were insignificant.The chemotyoes of EOs of fresh aerial parts of Houttuynia were closely related to the diversity of the chromosome numbers,while other chemical components were not related to the diversity of the chromosome numbers,obviously.No significant chemical component difference was detected between the H.cordata and H.emeiensis accessions.The different ehemotypes of EOs could cause the different effects on restraining the microbial species;and it was important to the drugs production and utlization,which used EOs of Houttuynia as the raw materials.
四川和重庆地区的鱼腥草资源分布很广,遗传多样性较大。鱼腥草作为药材或蔬菜主要是利用其中的化学成分,而鱼腥草种质的不同会对这些化学成分产生影响。正确有效地评估鱼腥草资源各种化学成分间的差异,有助于更合理地开发利用这些资源。本研究对四川和重庆地区的鱼腥草资源挥发油成分、黄酮类成分、维生素成分的组成和含量以及部分重金属和农药的残留量进行了检测与分析,获得到如主要结果:
1.对18份新鲜鱼腥草材料地上和地下部分挥发油化学成分进行气相色谱质谱联用(GC/MS)分析发现,鱼腥草鲜草地上部分的挥发油成分中共鉴定出21种成分,材料间挥发油成分具有很大的遗传差异。首次发现根据鱼腥草地上部分挥发油成分可分为两种化学型:即癸醛型(D型)和月桂烯型(M型)。聚类分析和主成分分析结果表明,鱼腥草挥发油化学型与染色体数目有关,染色体数目少于80的所有材料均为D型,染色体数目大于或等于80的材料均为M型。D型鱼腥草的单萜类和倍半萜成分含量很低,平均仅5.72%和0.16%,其它非萜类直链化合物占整个挥发油组成的76.94%至94.71%。M型鱼腥草的单萜类和倍半萜含量较高,平均分别为36.16%和1.38%,其它直链化合物的浓度范围为37.88%～64.89%。试验结果还表明,鱼腥草挥发油化学型与其地理来源无关。鱼腥草地下部分挥发油中共鉴定出16种化合物,其不同材料地下部分挥发油成分组成及含量间差异均较小。本试验还发现,蕺菜和峨眉蕺菜间挥发油成分差异不显著。
2.采用RAPD、RAMP、ISSR和PCR-RFLP分子标记技术分析鱼腥草资源遗传多样性与挥发油化学型间关系。结果发现,在评价鱼腥草挥发油化学型与遗传背景多样性间关系上,ISSR分子标记较RAPD、RAMP和PCR-RFLP标记更有效,其遗传距离聚类分析结果与挥发油化学型分类结果几乎一致。但Mental test检验结果表明,基于本试验所用各种分子标记的遗传距离与挥发油成分的欧几里得距离间相关均未达显著水平。
3.通过对鱼腥草挥发油的紫外(UV)吸收光谱分析发现,鱼腥草挥发油在乙酸乙酯溶剂中的紫外吸收曲线的最大吸收波长λmax主要在252 nm和267 nm;23份试样的紫外吸收曲线可分为5种类型,化学型与吸收曲线类型不尽一致。各供试样品在两波长下的吸光度差异显著,23份试样的吸光度可分为3类。相关分析结果表明,挥发油成分中各主要萜类化合物含量与两个吸收峰的吸收值均呈正相关,且大多数达到极显著水平,而各主要不饱和烷烃类化合物含量与两个吸收峰的吸收值均呈显著或极显著的负相关。
4.分别使用不同浓度的D型和M型挥发油对赤霉菌(Fusarium graminearum)、炭疽菌(Colletotrichum coccodes)、灰霉菌(Botrytis cinerea)、大肠杆菌(Escherichia coli)和金黄色葡萄球菌(Staphylococcus aureus)的抑菌能力进行比较。结果表明,两种化学型挥发油的抑菌能力差异显著。D型挥发油对供试的各菌种,特别是对真菌的抑制能力明显高于M型挥发油。此外,同一挥发油对不同菌种抑制能力不同。
5.采用GC/MS法对生产中鱼腥草主栽品系的鲜草地上部分挥发油化学成分变化进行检测。方差分析结果表明,w01-100和w01-94的挥发油主要化学成分含量在一天中日照时间内无显著变化,以w01-94挥发油主要化学成分含量更稳定,w01-100和w01-94在整个白天内均可采收。此外,鱼腥草地上部分鲜草挥发油的部分主要成分间含量变化相关显著,但不同材料间相同两种成分间的相关性可能不同。
6.采用紫外-可见(UV-VIS)吸收光谱法和薄层色谱(TLC)法对提取挥发油后的剩余水溶液中黄酮类成分分析。显色鉴定和紫外-可见光谱扫描结果发现,提取剩余液中的黄酮类成分的种类丰富,主要类群有黄酮类、黄酮醇类、异黄酮类、二氢黄酮类和二氢黄酮醇类。薄层色谱结果表明这些成分中以苷类为主,含有芦丁和金丝桃苷成分。14份鱼腥草材料总黄酮含量差异达到极显著水平,平均含量为1.93 mg/g,以w01-16的总黄酮含量最高,为3.09 mg/g。鱼腥草资源的黄酮类成分种类及总黄酮含量与其染色体数目间均无显著相关。提取鱼腥草挥发油后的水溶液回收再利用价值明显。
7.采用高效液相色谱(HPLC)法比较鱼腥草资源间槲皮素含量差异。采用HypersilODS C18(250mm×4.0mm 5μm)色谱柱,流动相乙腈:0.4%磷酸=45:55,调pH=3,流速为1.0ml/min,柱温30℃,紫外检测波长370nm。结果发现,槲皮素含量在22.43 ng～2.24 ug范围内具有良好的线性关系,回归方程为Y=1.261458X-16.1218(r=0.9997),鱼腥草材料间槲皮素含量差异显著,w01-120中槲皮素含量水平最高,达4.02 mg/g。
8.采用HPLC法对鱼腥草幼嫩植株的维生素种类及含量进行测定,脂溶性维生素VA、δ-VE、VD_2、VD_3、α-VE和VK_1采用Hyperclone BDS C_(18)(150 mm×4.6 mm,5μm)色谱柱,以甲醇:乙酸乙酯(95:5)为流动相,275 nm下测定;水溶性维生素VB_1、VB_2、VB_6、烟酸和VC采用Luna C_(18)(2)100(?)(200 mm×4.6 mm,5μm)色谱柱,柱温30℃,以0.005 mol·mL~(-1)己烷磺酸钠(pH=3.2):甲醇(7:3),254 nm下测定。结果发现,鱼腥草幼嫩植株主要含有较多的α-VE和VC、含少量的VD_3、VK_1以及烟酸,而其余6种维生素均未检测到。鱼腥草资源间维生素种类差异很小,但含量差异显著。其地上部分维生素种类和含量比地下部分丰富,即鱼腥草地上部分维生素的营养价值高于其地下部分。
9.采用原子荧光(AFS)法同时测定不同鱼腥草资源中砷(As)和汞(Hg)含量。结果发现,在土壤中As的含量水平为11.557mg/kg,Hg的含量为0.387mg/kg的栽培条件下,鱼腥草幼嫩地上部分中As平均含量为0.161 mg/kg,Hg平均含量为0.004 mg/kg,幼嫩地下部分中两者的平均含量分别为0.130 mg/kg和0.0025 mg/kg。鱼腥草资源间对As、Hg积累的差异不显著,其As、Hg的含量低于国标规定限量。
10.采用气相色谱(GC)法,按GB/T14551-2003测定666和DDT的含量。鱼腥草幼嫩地上部分中仅小部分材料中检出痕量的δ-666。鱼腥草资源间对666、DDT积累的差异不显著,其666、DDT的含量低于国标规定限量。
综上所述,鱼腥草资源间在挥发油、黄酮类、维生素等自身代谢化学成分上差异显著;在重金属含量和农药残留量上差异不显著。其中,鲜草挥发油成分化学型的产生与其染色体数目变异高度相关,其他化学成分间差异与其染色体数目变异关系不明显。蕺菜和峨眉蕺菜在化学成分上并无显著差异。挥发油化学型的不同会导致其抗菌效果的不同,这对以鱼腥草挥发油为原料的药品生产和使用有重要意义。
HERBA HOUTTYNIAE,is a kind of traditional Chinese medicine and also a kind of delicious vegetable.According to Chinese Pharmacopoeia(2005),the fresh aerial and underground parts and the dry aerial parts during flowering period could be used as Chinese medicine.In the meantime,the fresh young stems and leaves are also delicious vegetable in its main spreading regions.HERBA HOUTTYNIAE came from Houttuynia cordata Thunb., which belongs to genus Houttuynia of Saururaceae.H.cordata is the only species in Houttuynia Thunb.according to Florae Reipublicae Popularis Sinicae and Flora Sichuanica. Zhu et al.(2001)classified a new species H,emeiensis Z.Y.Zhu et S.L.Zhang in the genus Houttuynia collected at Emei mountain in Sichuan province.It was called commonly as white Yuxingcao,which was also used as vegetable and HERBA HOUTTYNIAE in Emei and Leshan regions.
Houttuynia is widely spread in Sichuan and Chongqing regions of China.There existed plentiful genetic variations among genus Houttuynia germplasm resources.The chemical components of genus Houttuynia is the main reason why people utilized it as a kind of medicinal plants or vegetables.The diversities among Houttuynia germplasm resources would influence on these chemical components.Evaluation correctly and effectively on the variations among the chemical components of Houttuynia resources will help to the exploitation and ulilization of Houttuynia resources.In this paper,the compositions and their contents of the essential oils, flavones,vitamins and the rudimental contents of some heavy metals and pesticides of Houttuynia accessions in Sichuan and Chongqing regions were analyzed.The main results were as follows:
1.The essential oil(EO)compositions of 18 fresh Houttuynia accessions from Sichuan and Chongqing in China were analyzed by using GC/MS method.In total,21 components could be identified from the essential oils(EO)of the aerial parts of Houttuynia.High level of chemical polymorphism in the EOs of the aerial parts of Houttuynia was observed.On the basis of GC/MS data of the EOs of the aerial parts,two chemotypes could be identified for the frist time within the investigated Houttuynia accessions as follows:chemotypes decanal(D) and myrcene(M).The results of cluster analysis(CA)and principal component analysis(PCA) showed that the chemotypes of Houttuynia were correlated with the chromosome numbers. All the accessions classified into chemotype D have the chromosome number less than 80, while chemotype M had the chromosome number equal to or more than 80.The chemotype D showed lower amounts of monoterpenes and sequiterpenes,on average of 5.72%and 0.16%, respectively.The other non-terpenic straight chain components made up 76.94%to 94.71%of the whole EO components.In general,the chemotype M showed higher amounts of monoterpenes(average 36.16%)and sequiterpenes(1.38%),while the concentration of other straight chain components rang was from 37.88%to 64.89%.There was no significant relationship between the chemotypes and their geographical distributions.Totally,16 compounds were characterized from the essential oils(EO)of the underground parts of Houttuynia.There was no significant chemical difference between the EO of the underground parts of the different Houttuynia accessions.In this study,no significant chemical component difference was detected between the H.cordata and H.emeiensis accessions.
2.The relationships between the genetic diversity among Houttuynia germplasm resources and the essential oil chemotypes were analyzed by using RAPD,RAMP,ISSR and PCR-RFLP DNA markers technology.The results showed that the ISSR marker was more effective to evaluate the relationships than RAPD,RAMP and PCR-RFLP markers on the evualation of the relationships between the genetic diversity and the essential oil chemotypes.The cluster results of genetic distances(GDs)based on ISSR marker were almost coincident with the cluster results of euclidean distances based on the essential oils chemical components.However,the results of matrix correlation showed,there were insignificant correlation between EDs based on the essential oils chemical components and GDs based on any kind of DNA markers.
3.Through analyzing the UV spectrum of the essential oil of Houttuynia,it was found that theλmax of the essential oil of Houttuynia were at 252 nm and 267 nm in acetic ether solvent. Twenty-three samples could be divided into 5 types,which could not be coincident with the essential oil chemotypes.The differences of the absorbency of these samples at 252 nm and 267 nm were remarkable.Twenty-three samples counld be divided into 3 types based on the results of absorbency.All of the correlations between the contents of terpenes of EOs and the absorbencies of EOs at the two wavelengths were positive,and most of them reached 1% remarkable levlel.But all the correlations between the contents of unsaturated alkane of EOs and the absorbencies were negative,and all of them reached 5%or 1%remarkable levlel.
4.The abilities to restrain the growths of Fusarium graminearum,Colletotrichum coccodes, Botrytis cinerea,Escherichia coli and Staphylococcus aureus were compared by using the different concentrations of D chemotype and M type essential oils.The results showed that the difference of the restraining abilities of the two chemotypes was obvious.The restraining abilities of D chemotype essential oil were stronger than that of M chemotype essential oil, especially to restrain the growths of the epiphytes.And the same chemotype EO had different abilities to restraine the different microbial species.
5.The essential oil constituents of the fresh aerial parts of two lines were analyzed by GC/MS.The results of variance analysis showed that the essential oil of the two new lines w01-100 and w01-94 did not change significantly during daytime.The contents of the main components of the essential oil of w01-94 were more stable.Lines w01-100 and w01-94 can be harvested during the whole daytime.There were significant correlations between the content changes of some main components of the essential oil of the fresh aerial parts of Houttuynia,but the correlations between the two same components might be different in different materials.
6.The flavone components in the residuary water solution after essential oil distillation were analyzed by using UV-VIS spectrum and TLC.The results of displaying colors identifications and UV-VIS spectrum showed that there were abundance flavone components in the residuary water solution,mainly included flavone,flavonol,isoflavone, dihydroflavone and dihydroflavonol.The results of TLC showed that most of these flavone components in the residuary water solution were glycoside components,containing rutin and hyperin.The average content of total flavone of 14 Houttuynia accessions was 1.93 mg/g, the content of w01-16 was the highest,reaching to 3.09 mg/g.The difference of the content of total flavone among the 14 Houttuynia accessions was distinct.There were insignificant correlationships between the types of total flavone components,contents of total flavone and the chromosome numbers of Houttuynia accessions.The recycled values of the residuary water solution were obvious.
7.The difference of quercetin content of different Houttuynia accessions was determined by using HPLC on Hypersil ODS C18(250mm×4.0mm 5μm)at 30℃with acetonitrile:0.4% phosphoric acid(45:55),pH=3 as the mobile phase.Detection wavelength was at 370nm. The flow rate was 1.0mL/min.The calibration curve of quercetin was 22.43ng～2.24ug(r= 0.999 7).The quercetin content of the accession w01-120 was the highest,arriving at 4.02mg/g.
8.The vitamin constituents and content of fresh young Houttuynia accessions were determined by using HPLC methods.The fat-soluble vitamins VA,δ-VE,VD_2,VD_3,α-VE and VK_1 were determined by Hyperclone BDS C_(18)column(150mm×4.6mm 5u)at 25℃with methanol:ethyl acetate(95:5)as the mobile phase at 275nm;the water-soluble vitamins VB_1, VB_2,VB_6,Nicotinic acid and V_C were determined by Luna C_(18)(2)100(?)column(200 mm×4.6mm 5u)at 30℃with 0.005mol/L hexane sulfonic sodium(pH 3.2):methanol(70:30) as the mobile phase.The flow rate was at 1.0 mL/min.Detection wavelength was set at 254nm to determine the content of VB_1,VB_2,VB_6,Nicotinic acid and V_C of Hauttuynia.The mainly vitamin constituents wereα-VE and VC,less of VD_3、VK_1 and Nicotinic acid in Houttuynia,others 6 vitamins not detected in this research.The vitamin constituents and contents of the aerial parts were more abundant than that of underground parts in Houttuynia. The difference of the vitamin constituents among Houttuynia resource were little,but were notable in the content of their vitamins.The nutrition value of the aerial parts was higher than that of underground parts.
9.The remains of As and Hg in the young aerial and underground parts of 10 Houttuynia accessions and the soil cultivated these Houttuynia accessions were determined simultaneously by using AFS.The results showed that the content of As was 11.557mg/kg, Hg was 0.387mg/kg in soil,the mean content of As was 0.161mg/kg and Hg was 0.004mg/kg in the young aerial parts.And in the underground parts,the mean content of As was 0.130mg/kg and Hg was 0.0025mg/kg.The diference of the remains As and Hg among the Houttuynia accessions was insignificant.According to GB4810-94 and GB2762-94,the remains of heavy metals As and Hg were not beyond the standard.
10.The remains of 666 and DDT in the young aerial and underground parts of 10 Houttuynia accessions and the soil were determined simultaneously by using GC.The results showed that the remains ofδ-666 in soil was 0.17ng/kg,but the trace remains of the young aerial and underground parts in several accessions were detected.According to GB/T14551-2003,the remains of pesticides 666 and DDT were not beyond the standard.
To sum up,the differences of the self-metablism chemical components,such as the essential oils,flavones and vitamins were significant among the Houttuynia germplasm resources.And the variations on the rudimental contents of some heavy metals and pesticides were insignificant.The chemotyoes of EOs of fresh aerial parts of Houttuynia were closely related to the diversity of the chromosome numbers,while other chemical components were not related to the diversity of the chromosome numbers,obviously.No significant chemical component difference was detected between the H.cordata and H.emeiensis accessions.The different ehemotypes of EOs could cause the different effects on restraining the microbial species;and it was important to the drugs production and utlization,which used EOs of Houttuynia as the raw materials.
引文
[1]《中国药典》.北京:化学工业出版社,2005,1,附录XD,62.
[2]《中国植物志》.北京:科学出版社,1982,20(1),8.
[3]《四川植物志》.成都:四川人民出版社,1981,1,126-127.
[4]祝正银,张士良.峨眉山蕺菜属药用植物一新种.植物研究,2001,21(1):1-2
[5]吴卫,郑有良,杨瑞武,等.国产蕺菜种质资源的染色体数目变异及核穿壁现象.植物分类学报,2003,41(3):245-257
[6]吴卫,郑有良,陈黎,等.川产鱼腥草种质资源的同工酶分析.中药材,2002,25(10):695-698
[7]吴卫,郑有良,陈黎,等.鱼腥草种质资源的RAPD分析.药学学报,2002,37(12):986-992
[8]Wu Wei,Zheng Youliang,Chen Li,et al.Genetic diversity among the germplasm resources of Houttuynia Thunb.in China based on RAMP markers.Genetic Resources and Crop Evolution.2005,52,473-482
[9]吴卫,郑有良,陈黎,等.利用ISSR标记分析鱼腥草种质资源的遗传多样性.世界科学技术-中药现代化,2003,5(1):70-77
[10]杨玉霞,吴卫,郑有良.蕺菜属不同材料间比较解剖学研究.广西植物,2003,23(5):429-435
[11]陈黎,吴卫,郑有良.鱼腥草游离氨基酸的组成及HPLC分析.氨基酸和生物资源,2004,26(1):20-23
[12]陈黎,吴卫,郑有良.鱼腥草种质资源氨基酸组成及含量分析.广西植物,2006,26(6):697-701
[13]陈黎,吴卫,郑有良.不同染色体数目鱼腥草挥发油成分的薄层色谱分析.中草药,2004,35(12):1399-1402
[14]Wu Wei,Zheng Youliang,Chen Li,et al.PCR-RFLP analysis of cpDNA and mtDNA in the genus Houttuynia in some areas of China.Hereditas,2005,142,1-9
[15]Wu Wei,Zheng Youliang,Chen Li,et al.Evaluation of genetic relationships in the genus Houttuynia Thunb.in China based on RAPD and ISSR markers.Biochemical Systematics and Ecology,2005,33(11):1141-1157
[16]齐迎春,胡诚,侯集瑞.鱼腥草栽培技术.特产研究,1997,4:60-61
[17]陈曾林.鱼腥草的开发利用与栽培方法.农牧产品开发,1997,7:14
[18]印保林.鱼腥草的栽培与开发.中国土特产,1998,3:12-13
[19]谢荣贵.野菜鱼腥草的开发利用价值及其栽培技术.湖北农业科学,1995,1:48-50
[20]吴卫,郑有良,杨瑞武,等.鱼腥草氮、磷、钾营养吸收和累积特性初探.中国中药杂志,26(10):695-698
[21]吴卫,郑有良,杨瑞武,等.重壤土上鱼腥草干物质积累研究.中药材,2002,25(1):5-8
[22]吴卫,郑有良,杨瑞武,等.不同播期和用种量对鱼腥草新品系产量质量的影响.中草药,2003,34(9):859-861
[23]刘香,王莉.人工栽培和黔产野生鱼腥草挥发油成分的研究.贵阳医学院学报,1997,22(4):361-362
[24]Suaghara S.,Sugawara S.Studied on the shift in weed vegetation in the maturation process of farms.Ⅱ.A wedd shift by the alteration of the soil acidity.Weed Research,1981,26(3):233-238
[25]戴冰,肖子曾.鱼腥草注射液的研究近况.中成药,1994,16(6):45-46
[26]林必杰.鱼腥草注射液制备工艺改进.中成药研究,1984,7:7-8
[27]唐莉,徐攀辉,吴卫,等.鱼腥草生产标准操作规程(草案).现代中药研究与实践,2003,17(3):29-32
[28]伍贤进,张俭.药用鱼腥草生产标准操作规程(试行),中药材,2005:35-38
[29]刘永漋,邓志芳.鱼腥草挥发油化学成分研究 植物学报,1979,21(3):244-249
[30]刘启泰,秀绍宗,赵胜利,等.鱼腥草注射液质量的研究.中草药通讯,1979,11:23-28
[31]姜新民,严拯宇,严雪芹.紫外二阶导数光谱法测定中药复方制剂中合成鱼腥草素的含量.中草药,1994,25(6):296-298
[32]吴少尉,董延奎.光度法测定鱼腥草中有效成分的质量分数.湖北民族学院学报(自然科学版),2002,20(2):59-60
[33]张辉,田英.鱼腥草中癸酰乙醛含量的测定.光谱实验室,2003,20(4):583-585
[34]殷霞,严雪芹.气相色谱法测定复方中药制剂中的鱼腥草素.中草药,1993,24(2):73-74
[35]于健东,胡玉霞,田金改.鱼腥草中甲基正壬酮的含量测定.药物分析杂志,1999,19(1):50-51
[36]马振菁,张韬.毛细管气相色谱法测定鱼腥草注射液中甲基正壬酮含量[J].福建医科大学学报,2004,38(4):495-496
[37]周晓英,武新华,刘波.唇香草中挥发油的理化性质研究.新疆医科大学学报,2003,26(3):259-260
[38]兰瑞芳,郑曦,林少琴,等.滨海前胡与白花前胡的化学成分比较.中药与天然药物,2000,12(2):45-47
[39]肖培根.新编中药志(第三卷).北京:化学工业出版社,2002:200-206
[40]Choe K H,Kwon S J,Jung D S,et al.A study on chemical composition of Saururaceae growing in Korea.(Ⅰ).On volatile constituents of Houttuynia cordata by GC and GC-MS method.Punsok Kwahak,1988,1(1):57-61
[41]郝晓燕,李零,丁智慧,等.黔产野生鱼腥草挥发油成分分析.云南植物研究,1995,15(3):350-352
[42]曾虹燕,蒋丽娟,张超英.鱼腥草挥发油化学成分.植物资源与环境学报,2003,12(3):50-52
[43]曾志,石建功,曾和平,等.有机质谱学在中药鱼腥草研究中的应用.分析化学,2003,31(4):399-404
[44]李晓蒙,徐位良,何新荣.穗产人工栽培鱼腥草挥发油化学成分研究.广东药学院学报,2004,20(1):7-8,13
[45]Qi Meiling,Ge Xiaoxia,Liang Minmin,et al.Flash gas chromatography for analysis of the volatile comdounds from Houttuynia cordataThunb..Analytica Chimica Acta,2004,527(1):69-72
[46]Liang Minmin,Qi Meiling,Zhang Changbin,et al.Gas chromatography-mass spectrometry analysis of volatile compounds from Houttuynia cordata Thunb.after extraction by solid-phase microextraction,flash evaporation and steam distillation.Analytica Chimica Acta,2005,531(1):97-104
[47]Meng J,Leung KSY,Jiang ZH,et al.Establishment of GC-MS fingerprint of fresh Houttuynia cordata.Chemical and Pharmaceutical Bulletin,2005,53(11):1484-1489
[48]Chengjian Xu,Yizeng Liang,Foo Tim Chau.Identification of essential components of Houttuynia cordata by gas chromatography/mass spectrometry and the integrated chemometric approach.Talanta,2005,68:108-115
[49]李晓宁,胡芸,周枝凤.湖南产鱼腥草挥发油GC/MS数据的分析.计算机与应用化学,2002,19(3):234-236
[50]Pham,Xuan Sinh;Cao,Van Thu;Dinh,Thi Thanh Thuy.Study of chemical components of Houttuynia cordata Thunb.[J].Tap chi cong nghe sinh hoc,1997(7):7-9
[51]Tran,Huy Thai;Tran,Minh Hoi;Nguyen,Thi Hien.Chemical composition of the essential oil from the aerial part of Houttuynia cordata Thunb.in Vietnam[J].Tap chi cong nghe sinh hoc,2004,44(2A),26-28
[52]罗琥捷,李临生,王军.干品鱼腥草挥发油成分分析[J].陕西中医,2005,26(2):170-172
[53]陈胜璜,汤艳红,周日宝,等.不同采收期鱼腥草中挥发油的提取及成分测定[J].中成药,2005,27(11):1333-1335
[54]Kang Jung-Mi,Cha In-Ho,Lee Young-Kuen,et al.Identification of volatile essential oil,and flavor characterization and antibacterial effect of fractions from Houttuynia cordata Thunb.Ⅱ.Flavor characterization and antibacterial effect of fraction from Houttuynia cordata Thunb.obtained by preparative HPLC.Korean Society of Food Science and Nutrition.1997,26(2):214-221
[55]伍贤进,蒋向辉,张俭,等.鱼腥草不同居群营养成分及抑菌效果比较.时珍国医国药,2006,17(12):2383-2384
[56]《中药辞海》编审工作委员会.中药辞海{第二卷}.北京:中国医药科技出版社,1996,976-980
[57]齐迎春,胡诚,田园政,等.鱼腥草茎叶营养成分对比分析.特产研究,2001,(4):45-46
[58]廖德胜,王敬勉.鱼腥草黄酮的制备及其应用研究.中国食品添加剂,2002,2:81-83
[59]王艳芳,王新华,朱宇同.槲皮素药理作用研究进展.天然产物研究与开发,2003,15(2):171-173
[60]刘文尧,梁爱君,白皓.高效液相色谱法测定鱼腥草中槲皮素的含量.解放军药学学报,1999,15(6):40-41
[61]边清泉,刘家琴,李松.不同品种鱼腥草中懈皮素含量的检测与比较.光谱实验室,2005,22(5):1118-1120
[62]吴卫.鱼腥草的研究进展.中草药,2001,32(4):367-368
[63]任玉翠,周彦钢,凌文娟,等.鱼腥草营养液的研制.食品与机械,1998,1:13-14
[64]吴三桥,李新生,周建军.薇菜、蕺菜中氨基酸及其他营养素含量的测定.氨基酸和生物资源,2000,22(3):65-67
[65]李道林,何方,马成泽,等.砷对土壤生物学活性及蔬菜毒性的影响.农业环境保护,2000,19(3):148-151
[66]王宏,徐智.汞在环境中的污染和迁移转化.内蒙古环境保护,2000,12(1):46-47
[67]高静,周日宝,王朝晖.鱼腥草的现代研究进展.湖南中医学院药学院,2005,25(6):60-151
[68]史蕙,任利斌.黔产鱼腥草挥发油抑菌作用的初步研究.贵阳中医学学院院报刊,1998,20(3):61
[69]李国利,庄玉辉,那学明,等.3种中药对分枝杆菌抗菌作用的研究.微生物学通报,1989,16(3):153-156
[70]陈于正.鱼腥草注射液处方与工艺研究.中成药,1991,13(8):2-3
[71]伍贤进,蒋向辉,张俭,等.鱼腥草不同居群营养成分及抑菌效果比较.时珍国医国药,2006,17(12):2383-2384
[72]付明,伍贤进,刘丹,等.鱼腥草挥发性油含量及抑菌效果的比较.四川中医,2007,25(7):36-39
[73]国家中医药管理局《中华本草》编委会.中华本草(精选本).上海:上海科技出版社,1998,605-610
[74]朱宇同,杨汝才,苏章,等.鱼腥草非挥发油提取物抗病毒作用的初步研究.中草药,1983,14(7):25-26
[75]李文,王少军,严敏,等.鱼腥草提取液抗流感、腮腺炎病毒效果观察.预防医学文献信息,1999,5(4):347-348
[76]郑宣鹤.青蒿素等4种中草药抑制出血热病毒的实验研究.湖南医科大学学报,1993,18(2):165-167.
[77]王潮临.鱼腥草等中草药对外周血中性粒细胞和单核细胞化学发光影响的研究.广西医科大学学报,1997,14(2):20-22
[78]宁耀瑜,柯美珍,周晓玲,等.雾化吸入鱼腥草提取液对大鼠呼吸道及全身免疫功能的影响.广西医科大学学报,1997,14(4):70-72
[79]苏学素,陈宗道,焦必林,等.我国常见食物及其成分的抗过敏作用研究.西南农业大学学报,2000,22(1):77-80
[80]周大兴,张红霞,李昌煜,等.鱼腥草油抗慢反应物质及平喘作用的研究.中成药,1991,13(6):31-32
[81]胡麟,陆维艾,张显杰,等.复方鱼腥草口服液对辐射损伤小鼠免疫功能及细胞遗传学的影响[J].解放军药学学报,2001,17(3):160-161
[82]胡麟,陆维艾,魏荣,等.复方鱼腥草口服液对辐射损伤后小鼠外周血相的影响.现代医药卫生,2001,17(7):514-515
[83]胡麟,沈正生,魏荣,等.复方鱼腥草口服液对辐射损伤小鼠机体抗应激功能的影响.解放军药学学报,2002,18(1):44-45
[84]江学良,权启镇,王东,等.鱼腥草治疗溃殇性结肠炎大鼠对结肠压力的影响.世界华人消化杂志,1999,7(7):639
[85]江西省第二人民医院药物组.合成鱼腥草素在大鼠体内的吸收、分布和排泄.中华医学杂志,1976,56(7):454
[86]刘延福.鱼腥草素在家兔体内含量测定及动力学过程研究.河南中医,1987,7(增刊):68
[87]余栋华.鱼腥草注射液治疗小儿合胞病毒性肺炎41例.现代中西医结合杂志,1999,8(12):1957
[88]宋峥,徐辉甫.鱼腥草注射液雾化吸入治疗小儿支气管肺炎60例.湖北中医杂志,1999,21(4):176
[89]赵钧,毕伟蓬.鱼腥草注射液穴位注射治疗哮喘慢性支气管炎100例.中医药学刊,2002,20(2):238
[90]连明珍.鱼腥草注射液治疗小儿急性上呼吸道感染150例.天津中医,2001,18(1):47
[91]马燕兰,韩忠福,王建荣,等.超声雾化吸入鱼腥草对呼吸道消炎抗菌作用临床研究.中草药,2001,32(4):334-337
[92]孙志佳,王胜全,刘小虹.鱼腥草注射液肺泡灌洗治疗支气管扩张合并肺部感染20例疗效观察.中国中西医结合急救杂志,1999,6(11):514-515
[93]王全利.鱼腥草注射液治疗妊娠期急性肾盂肾炎22例.现代中西医结合杂志,1999,8(9):1488
[94]简祖林.鱼腥草煎水坐浴治疗痔疮出血.贵州医药,1988,12(1):60
[95]马冰松.鱼腥草注射液治疗单纯疱疹性角膜炎88例.辽宁中医杂志,1998,(12):583
[96]曾自明,喻京生,李波.鱼腥草注射液治疗急性流行性角结膜炎临床观察.中国中医急症,2000,9(3):111
[97]张燕.鱼腥草注射液鼻窦灌注疗法治疗慢性鼻窦炎300例.新中医,2002,34(11):53-54
[98]闫才刚,颜宏伟,李曦.鱼腥草注射液鼻窦灌注疗法治疗慢性鼻窦炎300例.中华实用中西医杂志,2002,2(15):539
[99]王芸.鱼腥草注射液治疗急性化脓性中耳炎的体会.中国中西医结合耳鼻咽喉科杂志,2001,9(3):137
[100]王银涛.鱼腥草注射液治疗流行性腮腺炎.浙江中西医结合杂志,2001,11(9):585-586
[101]张菊英.鱼腥草治疗流行性腮腺炎60例疗效观察.河南医药信息,2002,10(15):48
[102]赵芳.鱼腥草注射液穴注治疗慢性盆腔炎500例.中国民间疗法,2003,11(10):16
[103]吴也平.鱼腥草注射液腹腔灌注治疗慢性盆腔炎40例.四川中医,2003,21(7):63-64
[104]高洪霞,陈桂雪.鱼腥草穴位注射治疗附件炎74例疗效观察.泰山医学院学报,19(4):356
[105]许文红.鱼腥草注射液冷喷法治疗面部激素依赖性皮炎56例.中国中医药科技,1996,6(3):198
[106]常克,吕斌,钟柏松.999鱼腥草注射液消除肾病综合征蛋白尿36例.辽宁中医杂志,2000,27(5):206-207
[107]江学良,权启镇,董兴成.鱼腥草对溃殇性结肠炎患者直肠肛门动力学的影响.中医药学报,2000,28(4):43-44
[108]秦大军,刘兆霞.内眼手术中应用鱼腥草注射液预防感染.国际眼科杂志,2003,3(3):138
[109]李正清,李昭惠.鱼腥草注射液防治剖宫产感染127例临床效果分析.中国药业,1999,8(12):27-28
[110]来艳雪,苟新元,雷文新.鱼腥草注射液治疗急性盆腔炎临床观察.现代中医药,2004,(1):29
[111]毛晓春.鱼腥草注射液致过敏性紫癜.浙江中医杂志,2003,(6):267
[112]张丽萍,张艳红,杨慧.鱼腥草注射液过敏致喉头水肿20例.云南医药,2004,25(1):59-60
[113]韩朝宏,梁月冬.155例鱼腥草注射液不良反应分析.药物流行病学杂志,2004,13(5):243
[114]张俭.鱼腥草与人体健康.生物学教学,2005,30(8):60
[115]张俊青.鱼腥草的药用和食疗.药膳食疗研究,2000,3:7
[116]邹光友.鱼腥草的食用开发试验.绵阳农专学报,1989,5(3):39-42
[117]杨瑞,张文学,曾里等.鱼腥草饮料的研制开发.四川食品与发酵,2001,(1):42-44
[118]杨小生,康文艺,梁东妮.鱼腥草苦丁茶保健饮料.食品科技,2001,(6):44-45
[119]吴天样,汤庆莉.鱼腥草、南瓜、刺梨醋酸发酵饮料的研制.粮油与食品机械,2001,5:35-36
[120]龚美珍.鱼腥草保健茶饮料的研制.2003,5:74-76
[121]肖玫,刘彪,赵仁铮.鱼腥草天然饮料的工艺研究.西南农业大学学报,2003,25(2):153-156
[122]柯范生等.鱼腥草含片的研制.食品机械,2003,2:25
[123]丁筑红,陈锐,班玉校.鱼腥草袋装方便食品加工.食品机械,1998,5:25
[124]张康健,董娟娥。药用植物次生代谢.西安:西北大学出版社,2001:27-43
[125]Kristina Loziene,Jone Vaiciuniene,Petras R.Venskutonis.Chemical composition of the essential oil of different varieties of thyme(Thymus pulegioides)growing wild in Lithuania[J].Biochemical systematics and ecology,2003,31:249-259
[125]Marc Corticchiato,Felix Tomi,Antoine Franiois Bernardini,et al.Composition and infraspecibc variability of essential oil from Thymus herba barona Lois..Biochemical systematics and ecology,1998,26:915-932
[127]Mauro Marotti,Roberta Piccaglia,Enrico Giovanelli.Differences in Essential Oil Composition of Basil(Ocimum basilicum L.)Italian Cultivars Related to Morphological Characteristics.Journal of Agriculture and Food Chemistry,1996,44:3926-3929
[128]Renee J.Grayer,Geoffrey C.Kite,Fiona J.Goldstone,et al.Infraspecific Taxonomy and Essential Oil Chemotypes in Sweet Basil,Ocimum basilicum.Phytochemistry,1996,43(5):1033-1039
[129]Maria Gorettide Vasconcelos Silva,Francisco Josede Abreu Matos,Maria Iracema Lacerda Machado,et al.Essential oils of Ocimum basilicum L.,O.basilicum,var.minimum L.and O.basilicum,var.purpurascens Benth.grown in north-eastern Brazil.Flavour and Fragrance Journal,2003,18:13-14
[130]Mariateresa Russo,Guido C.Galletti,Paola Bocchini,et al.Essential Oil Chemical Composition of Wild Populations of Italian Oregano Spice(Origanum vulgare ssp.hirtum(Link)Ietswaart):A Preliminary Evaluation of Their Use in Chemotaxonomy by Cluster Analysis.1.Inflorescences.Journal of Agriculture and Food Chemistry,1998,46:3741-3746
[131]M.J.Perez-Alonso,A.Velasco-Negueruela,J.Pala-Paul,et al.Variations in the essential oil composition of Artemisia pedemontana gathered in Spain:chemotype camphor-1,8-cineole and chemotype davanone[J].Biochemical systematics and ecology,2003,31:77-84
[132]Helen D.Skaltsa,Anna Mavrommati,Theophanis Constantinidis.A chemotaxonomic investigation of volatile consitituents in Stachys subsect.Swainsonianeae(Labiatae).Phytochemistry,2001,57:235-244
[133]Marjo Keskitalo,Eija Pehu,James E.Simon.Variation in volatile compounds from tansy (Tanacetum vulgate L.)related to genetic and morphological differences of genotypes.Biochemical systematics and ecology,2001,29:267-285
[134]Yidong Lei,Peng Nan,Tashi Tsering,et al.Interpopulation variability of rhizome essential oils in Rhodiola crenulata from Tibet and Yunnan,China.Biochemical systematics and ecology,2004,32:611-614
[135]Chunjie Tian,Peng Nan,Jiakuan Chen,et al.Volatile composition of Chinese Hippophae rhamnoides and its chemotaxonomic implications.Biochemical systematics and ecology,2004,32:431-441
[136]蔡金娜,张亮,王峥诗,等.蛇床果实中香豆类成分的变异及其规律.药学学报,1999,34:767
[137]周荣汉.国产野生薄荷挥发油化学组分变异及其化学型.植物资源与环境,1998,7(3):13-18
[138]王臣,邢秀芳,马立祥,等.栽培条叶龙胆各形态类型龙胆苦苷含量的测定.中国中药杂志,2004,29(9):841-843
[139]张亮,马国祥,张正行,等.中药石斛质量的化学模式识别.药学学报,1994,29(4):290
[140]秦海林,赵天增.核磁共振氢谱鉴别植物中药的研究.药学学报,1999,34(1):58
[141]秦海林,尚玉俊,赵伟,等.核磁共振氢谱法鉴别黄连研究.中草药,2000,31(1):48
[142]徐朝辉,杨松松,康廷国.同种不同产地牛蒡子DNA指纹图谱特征研究.中草药,2001,32(6):541-542
[143]黄璐琦,王敏,杨滨.栝楼农家品种苗期的分子标记鉴别.中国中药杂志,1999,34(9):642
[144]张荣,张步振,叶浩.用RAPD技术鉴定木蓝属生药.中国中药杂志,1997,22(2):72-73
[145]曹晖,毕培曦,邵鹏柱.中药材苦地胆的DNA指纹鉴定.中药材,1996,19(12):608-611
[146]Shaw P C,But P H.Authentication of Panax species and their adulterants by random-primed polymerase chain reaction.Planta medica,1995,61:466-469
[147]黄璐琦.用随机扩增多态性DNA(RAPD)技术鉴别中药材天花粉及其类似品.药物分析杂志,1999,19(4):233-238
[148]张铭,黄华荣,廖苏梅,等.石斛属RAPD分析及鉴定铁皮石斛的特异性引物设计.中国中药杂志,2001,26(7):442-447
[149]郭兰萍,黄璐琦,王敏,等.南北苍术的RAPD分析机器划分的初步探讨.中国中药杂志,2001, 26(3):156-158
[150]陈毓亨,白守梅,程克棣,等.温郁金和川郁金的RAPD分析.中国中药杂志,1999,24(3):131-133
[151]马小军,汪小全,徐昭玺,等.人参不同栽培群体遗传关系的RAPD分析.植物学报,2000,42(6):587-590
[152]马小军,汪小全,孙三省,等.野生人参RAPD指纹研究.药学学报,1999,34(4):312-316
[153]黄璐琦,王敏,傅桂芳,等.中药白芷种质资源的RAPD分析.中国中药杂志,1999,24(8):457
[154]Yamazaki M,Sato A,Shimomura K,et al.Genetic relationships among Glycyrrhiza plants determined by RAPD and RFLP analysis.Biological and Pharmaceutical Bulletin,1994,17(11):1529-1531
[155]郭宝林,吴孟,斯金平,等.厚朴DNA分子标记的研究-正品的RAPD研究.药学学报,2001,36(5):386-389
[156]陈林姣,屈良鹄,施苏华,等.RAPD技术在溪黄草类原植物鉴别中的应用.中国中药杂志,1998,23:328
[157]陈永久,王文,杨跃雄,等.冬虫夏草(Cordyceps sinensis)的随机扩增多态性DNA及其遗传分化.遗传学报,1997,24:410
[158]Kitanaka S,Kunishi M,Taira T,et al.Ginsenoside production tissue culture and induction of regeneration of interspecific hybrid ginseng(Panax ginseng×P.quinquefolium).Natural Medicines,1997,51(1):1
[159]赵欢,吴卫,郑有良,等.应用RAMP分子标记研究红花资源遗传多样性.植物遗传资源学报,2007,8(1):64-71
[160]Zietkiwicz E,A Rafalski,D Labuda.Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification.Genomics,1994,20:176-183
[161]Gupta M,Chyi Y S,Romero-Severson J,et al.Amplification of DNA markers from evolutionarily diverse genomes using single primers of simple-sequence repeats.Theoretical and Applied Genetics,1994,89:998-1006
[162]Goodwin I D,Aitken E A B,Smith L W.Application of inter-simple sequence repeat(ISSR)markers to plant genetics.Electrophoresis,1997,18:1524-1528
[163]陈祖云,王晓丽,宁聚先.贵州天麻遗传多样性的ISSR初步分析.中华中医药杂志,2007,22(7):436-439
[164]张君毅,郭巧生,杭悦宇.半夏ISSR-PCR反应体系的建立及条件优化研究.江苏农业科学,2007,2:154-157
[165]彭帅,伍贤进,罗碉佶,等.17份鱼腥草种质亲缘关系的ISSR分析.安徽农业科学,2007,35(12):3484-3486
[166]陈志彤,郑伟文,肖华山.泽泻26SrDNA的扩增及酶切反应条件优化.福建农业学报,2004,19(2):117-120
[167]褚必海,丁小余,李雪霞,等.保健食品建泽泻及其混淆品的PCR-RFLP分子鉴别.食品科学, 2007,28(2):177-181
[168]张婷,徐珞珊,王峥涛,等.药用植物束花石斛、流苏石斛及其形态相似种的PCR-RFLP鉴别研究.药学学报,2005,40(8):728-733
[169]康卓.中国生物源农药产业化进展.农药,2001,40(3):4-8
[170]张兴.试论无公害农药.西北农业大学学报,1995,23(6):90-95
[171]何静,沈慧敏,张新虎.七种植物粗提物对常见植物病菌的抑菌作用研究.甘肃农业大学学报,2004.4:447-450
[172]王冬梅,朱玮,张存莉,等.卷叶黄精对植物病原菌的抗菌活性研究.西北植物学报,2006,26(7):1473-1477
[173]黄奔立,朱华,顾艳,等.中草药对5种蔬菜病菌的抑制作用.扬州大学学报(农业与生命科学版),2006,27(3):84-86
[174]熊大胜,席在星,邓应威.鱼腥草提取物抑菌作用研究.常德师范学院学报(自然科学版),2002,14(4):59-60
[175]孟昭礼,吴献忠,高庆霄,等.银杏提取液对4种植物病原菌的抑菌作用.植物病理学报,1995,25(4):357-360
[176]孟昭礼,罗兰,尚坚,等.人工模拟杀菌荆绿帝对8种植物病原菌的室内生测.莱阳农学院学报,1998,15(3):159-162
[177]孟昭礼,罗兰,尚坚,等.人工模拟杀菌剂银泰对10种植物病原菌的室内生测.莱阳农学院学报[J],1999,16(2):124-126
[178]孟昭礼,袁忠林,罗兰,等.人工模拟农用杀菌剂银泰对5种植物害的药效试验.莱阳农学院学报[J],2001,18(4):302-305
[179]孟昭礼,罗兰,袁忠林,等.人工模拟的植物源杀菌剂银泰防治番茄3种病害效果研究.中国农业科学[J],2002,35(7):863-866
[180]Nicolas Baldovini,Dominique Ristorcelli,Felix Tomi,et al.Infraspecific variability of the essential oil of Calamintha nepeta from Corsica(France).Flavour and Fragrance Journal,2000,15,50-54
[181]Giorgio,Pintore;Marianna,Usai;Pascale,Bradesi;et al.Chemical composition and antimicrobial activity of Rosmarinus officinalis L.oils from Sardinia and Corsica.Flavour and Fragrance Journal,2002,17,15-19
[182]Vincenzo,De Feo;Maurizio,Bruno;Bochra,tahiri;et al.Chemical Composition and Antibacterial Activity of EssentialOils from Thymus spinulosus Ten.(Lamiaceae).Journal of Agriculture and Food Chemistry,2003,51,3849-3853
[183]吕世民,粱可钧,葛传吉,等.怀牛膝多倍体育种的研究.中国中药杂志,1988,13(7):11
[184]乔传卓,崔熙.药用植物多倍体的应用.中药材,1981,4:40
[185]乔传卓,吴美枢,戴富宝,等.菘蓝多倍体育种的研究.植物学报,1989,31(9):678
[186]梁汉兴.论三白草科的系统演化和地理分布.云南植物研究,1995,17(3):255-267
[187]黄春燕,吴卫,郑有良,等.两种化学型鱼腥草不同生育期光合速率和挥发油化学成分的比较 研究.中国农业科学,2007,40(6):1150-1158
[188]Sαnchez de la Hoz,M.P.;Davila,J.A.;Loarce,Y.;et al.Simple sequence repeat primers used in polymerase chain reaction amplification to study genetic diversity in barley.Genome,1996,39,112-117
[189]Divaret,I.;Margale,E.;Thomas,G.;et al.RAPD markers on seed bulks efficiently assess the genetic diversity of a Brassica oleracea L.Theoretical and Applied Genetics,1999,98,1029-1035
[190]Nebauer,S.G.;Del CastilloAgudo,L.;Segura,J.;et al.RAPD variation within and among natural populations of outcrossing willow-leaved foxglove(Digitalis obscura L.).Theoretical and Applied Genetics,1999,98,985-994
[191]Parani,M.;Lakshmi,M.;Ziegenhagen,B.,et al.Molecular phylogeny of mangrove.Ⅵ.PCR-RFLP of trnS-psbC and rbcL gene regions in 24 mangrove and mangrove associate species.Theoretical and Applied Genetics,2000,100,456-460.
[192]Xu.D.H.,Abe.A.,Kanazawa.A.,et al.Identification of sequence variations by PCR-RFLP and its application to the evaluation of cpDNA diversity in wild and cultivated soybeans.Theoretical and Appliod Genetics,2001,102,683-688.
[193]Roberto,F.Vieira;Renee,J.Grayer;Alan,Paton;et al.Genetic diversity of Ocimum gratissimum L.based on volatile oil constituents,flavonoids and RAPD markers.Biochemical Systematics and Ecology,2001,29,287-304
[194]S.Echeverrigaray,G.Agostini,L.Atti-Serfini,et al.Correlation between the chemical and genetic relationships among commercial Thyme cultivars.Journal of Agriculture and Food Chemistry,2001,49,4220-4223
[195]Yuan Hu,Qiaoyan Zhang,Hailiang Xin,et al.Association between chemical and genetic variation of Vitex rotundifolia populations from different locations in China:its implication for quality control of medicinal plants.Biomedical Chromatography,2007,21(9):
[196]Madoka Hosoi,Michiho Ito,Toru Yagura,Robert Phillip Adams,et al.cDNA isolation and functional expression of myrcene synthase from Perilla frutescens.Biological and Pharmaceutical Bulletin,2004,27(12):1979-1985
[197]Natalia Dudareva,Diane Martin,Christine M.Kish,et al.(E)-ocimene and myrcene synthase genes of floral scent biosynthesis in Snapdragon:Function and expression of three terpene synthase genes of a new terpene synthase subfamily the plant cell.American Society of Plant Biologists,2003,15,1227-1241
[198]Shan Lu,Ran Xu,Jun-Wei Jia,et al.Cloning and functional characterization of a β-pinene synthase from Artemisia annua that shows a circadian pattern of expressionl plant physiology.American Society of Plant Biologists,2002,130,477-486
[199]Takuro Maruyama,Michiho Ito,Fumiyuki Kiuchi,et al.Molecular cloning,functional expression and characterization of d-Limonene synthase from Schizonepeta tenuifolia.Biological and Pharmaceutical Bulletin,2001,24(4),373-377
[200]王宁生,刘启德,冯美蓉.导数光谱法在益智仁挥发油定性鉴别中的应用.广州中医学院学报, 1990,7(4):225-227
[201]田雅琴,王新霞,高丽红.川芎挥发油稳定性考察.上海中医药杂志,2003,37(7):49-52
[202]黄春燕,吴卫,郑有良.鱼腥草不同部位挥发油化学成分的比较.药物分析杂志,2007,27(1):40-44
[203]朱明华.仪器分析第三版.北京:高等教育出版社,2000:280-282
[204]陈丽艳,崔志恒.植物精油抗菌活性的研究进展.黑龙江医药,2006,19(3):197-198
[205]Cox S D,Mamrn C M,Markham J L.Interactions between components of the essential oil of Melaleuca altermifolia.Journal of Applied Microbial,2001,91(3):492-497
[206]申利群,莫洪波,尹笃林,等.几种单环单萜化合物在农药中的应用.农药,2004,43(4):153-156
[207]秦燕.几种植物精油的抗菌效果研究.食品与机械,1997(2):21-22
[208]钟凤林,陈和荣,陈敏.青蒿最佳采收时期、采收部位和干燥方式的实验研究.中国中药杂志,1997,22(7):405
[209]岳凤先.中药的现代研究.北京:中医古籍出版社,1988:32
[210]杨继祥,李寿乔,齐桂元.药用植物栽培学.北京:农业出版社,1993:344
[211]吴卫,郑有良,马勇,等.鱼腥草不同居群产量和质量分析.中国中药杂志,2003,28(8):718-720
[212]马柏林,梁淑芳,董娟娥,等.杜仲黄酮的微乳薄层色谱鉴定研究.西北林学院学报,2001,16(2):72-74
[213]康纯,闻莉毓,丁仲伯.微乳薄层色谱用于黄酮类成分分离鉴定的研究.药物分析杂志,2000,20(2):121-124
[214]池静端,何秀峰,刘爱茹,等.HPLC法测定银杏叶中6种黄酮成分的含量.药学学报,1997,32(8):625-628
[215]鲁鑫焱,张超,赵怀清,等.不同产地胡芦巴中总黄酮和槲皮素的含量测定.沈阳药科大学学报,2004,21(6):430-433
[216]余红,徐礼.RP-HPLC法测定红花中黄酮醇的含量.药学学报,1997,32(2):120-122
[217]齐迎春,胡诚,等.药食兼用鱼腥草.中国林副特产,1997,12(14):43-45
[218]Owen.R.Fenneman.食品化学.北京:中国轻工业出版社,2003,443
[219]于连贵,李雪花,高山,等.平贝母等3种中药材铅、镉、砷、汞含量的研究.吉林农业大学学报,2001,23(2):53-56
[220]王金平,王晓滨.化妆品、食物等日用品中微、痕量铅、As、Hg的测定.日用化工工业,1997,(6):39-40
[221]聂燕,李缙扬,秦宏伟.应用AFS-2201型双道原子荧光光谱法联合测定饲料中As和Hg.中国饲料,1997(7):32-34
[222]索有瑞.氢化-AFS法测定青藏高原中草药中的微量As、锑、硒和Hg.光谱学与光谱分析,1997,17(5):103-107
[2]《中国植物志》.北京:科学出版社,1982,20(1),8.
[3]《四川植物志》.成都:四川人民出版社,1981,1,126-127.
[4]祝正银,张士良.峨眉山蕺菜属药用植物一新种.植物研究,2001,21(1):1-2
[5]吴卫,郑有良,杨瑞武,等.国产蕺菜种质资源的染色体数目变异及核穿壁现象.植物分类学报,2003,41(3):245-257
[6]吴卫,郑有良,陈黎,等.川产鱼腥草种质资源的同工酶分析.中药材,2002,25(10):695-698
[7]吴卫,郑有良,陈黎,等.鱼腥草种质资源的RAPD分析.药学学报,2002,37(12):986-992
[8]Wu Wei,Zheng Youliang,Chen Li,et al.Genetic diversity among the germplasm resources of Houttuynia Thunb.in China based on RAMP markers.Genetic Resources and Crop Evolution.2005,52,473-482
[9]吴卫,郑有良,陈黎,等.利用ISSR标记分析鱼腥草种质资源的遗传多样性.世界科学技术-中药现代化,2003,5(1):70-77
[10]杨玉霞,吴卫,郑有良.蕺菜属不同材料间比较解剖学研究.广西植物,2003,23(5):429-435
[11]陈黎,吴卫,郑有良.鱼腥草游离氨基酸的组成及HPLC分析.氨基酸和生物资源,2004,26(1):20-23
[12]陈黎,吴卫,郑有良.鱼腥草种质资源氨基酸组成及含量分析.广西植物,2006,26(6):697-701
[13]陈黎,吴卫,郑有良.不同染色体数目鱼腥草挥发油成分的薄层色谱分析.中草药,2004,35(12):1399-1402
[14]Wu Wei,Zheng Youliang,Chen Li,et al.PCR-RFLP analysis of cpDNA and mtDNA in the genus Houttuynia in some areas of China.Hereditas,2005,142,1-9
[15]Wu Wei,Zheng Youliang,Chen Li,et al.Evaluation of genetic relationships in the genus Houttuynia Thunb.in China based on RAPD and ISSR markers.Biochemical Systematics and Ecology,2005,33(11):1141-1157
[16]齐迎春,胡诚,侯集瑞.鱼腥草栽培技术.特产研究,1997,4:60-61
[17]陈曾林.鱼腥草的开发利用与栽培方法.农牧产品开发,1997,7:14
[18]印保林.鱼腥草的栽培与开发.中国土特产,1998,3:12-13
[19]谢荣贵.野菜鱼腥草的开发利用价值及其栽培技术.湖北农业科学,1995,1:48-50
[20]吴卫,郑有良,杨瑞武,等.鱼腥草氮、磷、钾营养吸收和累积特性初探.中国中药杂志,26(10):695-698
[21]吴卫,郑有良,杨瑞武,等.重壤土上鱼腥草干物质积累研究.中药材,2002,25(1):5-8
[22]吴卫,郑有良,杨瑞武,等.不同播期和用种量对鱼腥草新品系产量质量的影响.中草药,2003,34(9):859-861
[23]刘香,王莉.人工栽培和黔产野生鱼腥草挥发油成分的研究.贵阳医学院学报,1997,22(4):361-362
[24]Suaghara S.,Sugawara S.Studied on the shift in weed vegetation in the maturation process of farms.Ⅱ.A wedd shift by the alteration of the soil acidity.Weed Research,1981,26(3):233-238
[25]戴冰,肖子曾.鱼腥草注射液的研究近况.中成药,1994,16(6):45-46
[26]林必杰.鱼腥草注射液制备工艺改进.中成药研究,1984,7:7-8
[27]唐莉,徐攀辉,吴卫,等.鱼腥草生产标准操作规程(草案).现代中药研究与实践,2003,17(3):29-32
[28]伍贤进,张俭.药用鱼腥草生产标准操作规程(试行),中药材,2005:35-38
[29]刘永漋,邓志芳.鱼腥草挥发油化学成分研究 植物学报,1979,21(3):244-249
[30]刘启泰,秀绍宗,赵胜利,等.鱼腥草注射液质量的研究.中草药通讯,1979,11:23-28
[31]姜新民,严拯宇,严雪芹.紫外二阶导数光谱法测定中药复方制剂中合成鱼腥草素的含量.中草药,1994,25(6):296-298
[32]吴少尉,董延奎.光度法测定鱼腥草中有效成分的质量分数.湖北民族学院学报(自然科学版),2002,20(2):59-60
[33]张辉,田英.鱼腥草中癸酰乙醛含量的测定.光谱实验室,2003,20(4):583-585
[34]殷霞,严雪芹.气相色谱法测定复方中药制剂中的鱼腥草素.中草药,1993,24(2):73-74
[35]于健东,胡玉霞,田金改.鱼腥草中甲基正壬酮的含量测定.药物分析杂志,1999,19(1):50-51
[36]马振菁,张韬.毛细管气相色谱法测定鱼腥草注射液中甲基正壬酮含量[J].福建医科大学学报,2004,38(4):495-496
[37]周晓英,武新华,刘波.唇香草中挥发油的理化性质研究.新疆医科大学学报,2003,26(3):259-260
[38]兰瑞芳,郑曦,林少琴,等.滨海前胡与白花前胡的化学成分比较.中药与天然药物,2000,12(2):45-47
[39]肖培根.新编中药志(第三卷).北京:化学工业出版社,2002:200-206
[40]Choe K H,Kwon S J,Jung D S,et al.A study on chemical composition of Saururaceae growing in Korea.(Ⅰ).On volatile constituents of Houttuynia cordata by GC and GC-MS method.Punsok Kwahak,1988,1(1):57-61
[41]郝晓燕,李零,丁智慧,等.黔产野生鱼腥草挥发油成分分析.云南植物研究,1995,15(3):350-352
[42]曾虹燕,蒋丽娟,张超英.鱼腥草挥发油化学成分.植物资源与环境学报,2003,12(3):50-52
[43]曾志,石建功,曾和平,等.有机质谱学在中药鱼腥草研究中的应用.分析化学,2003,31(4):399-404
[44]李晓蒙,徐位良,何新荣.穗产人工栽培鱼腥草挥发油化学成分研究.广东药学院学报,2004,20(1):7-8,13
[45]Qi Meiling,Ge Xiaoxia,Liang Minmin,et al.Flash gas chromatography for analysis of the volatile comdounds from Houttuynia cordataThunb..Analytica Chimica Acta,2004,527(1):69-72
[46]Liang Minmin,Qi Meiling,Zhang Changbin,et al.Gas chromatography-mass spectrometry analysis of volatile compounds from Houttuynia cordata Thunb.after extraction by solid-phase microextraction,flash evaporation and steam distillation.Analytica Chimica Acta,2005,531(1):97-104
[47]Meng J,Leung KSY,Jiang ZH,et al.Establishment of GC-MS fingerprint of fresh Houttuynia cordata.Chemical and Pharmaceutical Bulletin,2005,53(11):1484-1489
[48]Chengjian Xu,Yizeng Liang,Foo Tim Chau.Identification of essential components of Houttuynia cordata by gas chromatography/mass spectrometry and the integrated chemometric approach.Talanta,2005,68:108-115
[49]李晓宁,胡芸,周枝凤.湖南产鱼腥草挥发油GC/MS数据的分析.计算机与应用化学,2002,19(3):234-236
[50]Pham,Xuan Sinh;Cao,Van Thu;Dinh,Thi Thanh Thuy.Study of chemical components of Houttuynia cordata Thunb.[J].Tap chi cong nghe sinh hoc,1997(7):7-9
[51]Tran,Huy Thai;Tran,Minh Hoi;Nguyen,Thi Hien.Chemical composition of the essential oil from the aerial part of Houttuynia cordata Thunb.in Vietnam[J].Tap chi cong nghe sinh hoc,2004,44(2A),26-28
[52]罗琥捷,李临生,王军.干品鱼腥草挥发油成分分析[J].陕西中医,2005,26(2):170-172
[53]陈胜璜,汤艳红,周日宝,等.不同采收期鱼腥草中挥发油的提取及成分测定[J].中成药,2005,27(11):1333-1335
[54]Kang Jung-Mi,Cha In-Ho,Lee Young-Kuen,et al.Identification of volatile essential oil,and flavor characterization and antibacterial effect of fractions from Houttuynia cordata Thunb.Ⅱ.Flavor characterization and antibacterial effect of fraction from Houttuynia cordata Thunb.obtained by preparative HPLC.Korean Society of Food Science and Nutrition.1997,26(2):214-221
[55]伍贤进,蒋向辉,张俭,等.鱼腥草不同居群营养成分及抑菌效果比较.时珍国医国药,2006,17(12):2383-2384
[56]《中药辞海》编审工作委员会.中药辞海{第二卷}.北京:中国医药科技出版社,1996,976-980
[57]齐迎春,胡诚,田园政,等.鱼腥草茎叶营养成分对比分析.特产研究,2001,(4):45-46
[58]廖德胜,王敬勉.鱼腥草黄酮的制备及其应用研究.中国食品添加剂,2002,2:81-83
[59]王艳芳,王新华,朱宇同.槲皮素药理作用研究进展.天然产物研究与开发,2003,15(2):171-173
[60]刘文尧,梁爱君,白皓.高效液相色谱法测定鱼腥草中槲皮素的含量.解放军药学学报,1999,15(6):40-41
[61]边清泉,刘家琴,李松.不同品种鱼腥草中懈皮素含量的检测与比较.光谱实验室,2005,22(5):1118-1120
[62]吴卫.鱼腥草的研究进展.中草药,2001,32(4):367-368
[63]任玉翠,周彦钢,凌文娟,等.鱼腥草营养液的研制.食品与机械,1998,1:13-14
[64]吴三桥,李新生,周建军.薇菜、蕺菜中氨基酸及其他营养素含量的测定.氨基酸和生物资源,2000,22(3):65-67
[65]李道林,何方,马成泽,等.砷对土壤生物学活性及蔬菜毒性的影响.农业环境保护,2000,19(3):148-151
[66]王宏,徐智.汞在环境中的污染和迁移转化.内蒙古环境保护,2000,12(1):46-47
[67]高静,周日宝,王朝晖.鱼腥草的现代研究进展.湖南中医学院药学院,2005,25(6):60-151
[68]史蕙,任利斌.黔产鱼腥草挥发油抑菌作用的初步研究.贵阳中医学学院院报刊,1998,20(3):61
[69]李国利,庄玉辉,那学明,等.3种中药对分枝杆菌抗菌作用的研究.微生物学通报,1989,16(3):153-156
[70]陈于正.鱼腥草注射液处方与工艺研究.中成药,1991,13(8):2-3
[71]伍贤进,蒋向辉,张俭,等.鱼腥草不同居群营养成分及抑菌效果比较.时珍国医国药,2006,17(12):2383-2384
[72]付明,伍贤进,刘丹,等.鱼腥草挥发性油含量及抑菌效果的比较.四川中医,2007,25(7):36-39
[73]国家中医药管理局《中华本草》编委会.中华本草(精选本).上海:上海科技出版社,1998,605-610
[74]朱宇同,杨汝才,苏章,等.鱼腥草非挥发油提取物抗病毒作用的初步研究.中草药,1983,14(7):25-26
[75]李文,王少军,严敏,等.鱼腥草提取液抗流感、腮腺炎病毒效果观察.预防医学文献信息,1999,5(4):347-348
[76]郑宣鹤.青蒿素等4种中草药抑制出血热病毒的实验研究.湖南医科大学学报,1993,18(2):165-167.
[77]王潮临.鱼腥草等中草药对外周血中性粒细胞和单核细胞化学发光影响的研究.广西医科大学学报,1997,14(2):20-22
[78]宁耀瑜,柯美珍,周晓玲,等.雾化吸入鱼腥草提取液对大鼠呼吸道及全身免疫功能的影响.广西医科大学学报,1997,14(4):70-72
[79]苏学素,陈宗道,焦必林,等.我国常见食物及其成分的抗过敏作用研究.西南农业大学学报,2000,22(1):77-80
[80]周大兴,张红霞,李昌煜,等.鱼腥草油抗慢反应物质及平喘作用的研究.中成药,1991,13(6):31-32
[81]胡麟,陆维艾,张显杰,等.复方鱼腥草口服液对辐射损伤小鼠免疫功能及细胞遗传学的影响[J].解放军药学学报,2001,17(3):160-161
[82]胡麟,陆维艾,魏荣,等.复方鱼腥草口服液对辐射损伤后小鼠外周血相的影响.现代医药卫生,2001,17(7):514-515
[83]胡麟,沈正生,魏荣,等.复方鱼腥草口服液对辐射损伤小鼠机体抗应激功能的影响.解放军药学学报,2002,18(1):44-45
[84]江学良,权启镇,王东,等.鱼腥草治疗溃殇性结肠炎大鼠对结肠压力的影响.世界华人消化杂志,1999,7(7):639
[85]江西省第二人民医院药物组.合成鱼腥草素在大鼠体内的吸收、分布和排泄.中华医学杂志,1976,56(7):454
[86]刘延福.鱼腥草素在家兔体内含量测定及动力学过程研究.河南中医,1987,7(增刊):68
[87]余栋华.鱼腥草注射液治疗小儿合胞病毒性肺炎41例.现代中西医结合杂志,1999,8(12):1957
[88]宋峥,徐辉甫.鱼腥草注射液雾化吸入治疗小儿支气管肺炎60例.湖北中医杂志,1999,21(4):176
[89]赵钧,毕伟蓬.鱼腥草注射液穴位注射治疗哮喘慢性支气管炎100例.中医药学刊,2002,20(2):238
[90]连明珍.鱼腥草注射液治疗小儿急性上呼吸道感染150例.天津中医,2001,18(1):47
[91]马燕兰,韩忠福,王建荣,等.超声雾化吸入鱼腥草对呼吸道消炎抗菌作用临床研究.中草药,2001,32(4):334-337
[92]孙志佳,王胜全,刘小虹.鱼腥草注射液肺泡灌洗治疗支气管扩张合并肺部感染20例疗效观察.中国中西医结合急救杂志,1999,6(11):514-515
[93]王全利.鱼腥草注射液治疗妊娠期急性肾盂肾炎22例.现代中西医结合杂志,1999,8(9):1488
[94]简祖林.鱼腥草煎水坐浴治疗痔疮出血.贵州医药,1988,12(1):60
[95]马冰松.鱼腥草注射液治疗单纯疱疹性角膜炎88例.辽宁中医杂志,1998,(12):583
[96]曾自明,喻京生,李波.鱼腥草注射液治疗急性流行性角结膜炎临床观察.中国中医急症,2000,9(3):111
[97]张燕.鱼腥草注射液鼻窦灌注疗法治疗慢性鼻窦炎300例.新中医,2002,34(11):53-54
[98]闫才刚,颜宏伟,李曦.鱼腥草注射液鼻窦灌注疗法治疗慢性鼻窦炎300例.中华实用中西医杂志,2002,2(15):539
[99]王芸.鱼腥草注射液治疗急性化脓性中耳炎的体会.中国中西医结合耳鼻咽喉科杂志,2001,9(3):137
[100]王银涛.鱼腥草注射液治疗流行性腮腺炎.浙江中西医结合杂志,2001,11(9):585-586
[101]张菊英.鱼腥草治疗流行性腮腺炎60例疗效观察.河南医药信息,2002,10(15):48
[102]赵芳.鱼腥草注射液穴注治疗慢性盆腔炎500例.中国民间疗法,2003,11(10):16
[103]吴也平.鱼腥草注射液腹腔灌注治疗慢性盆腔炎40例.四川中医,2003,21(7):63-64
[104]高洪霞,陈桂雪.鱼腥草穴位注射治疗附件炎74例疗效观察.泰山医学院学报,19(4):356
[105]许文红.鱼腥草注射液冷喷法治疗面部激素依赖性皮炎56例.中国中医药科技,1996,6(3):198
[106]常克,吕斌,钟柏松.999鱼腥草注射液消除肾病综合征蛋白尿36例.辽宁中医杂志,2000,27(5):206-207
[107]江学良,权启镇,董兴成.鱼腥草对溃殇性结肠炎患者直肠肛门动力学的影响.中医药学报,2000,28(4):43-44
[108]秦大军,刘兆霞.内眼手术中应用鱼腥草注射液预防感染.国际眼科杂志,2003,3(3):138
[109]李正清,李昭惠.鱼腥草注射液防治剖宫产感染127例临床效果分析.中国药业,1999,8(12):27-28
[110]来艳雪,苟新元,雷文新.鱼腥草注射液治疗急性盆腔炎临床观察.现代中医药,2004,(1):29
[111]毛晓春.鱼腥草注射液致过敏性紫癜.浙江中医杂志,2003,(6):267
[112]张丽萍,张艳红,杨慧.鱼腥草注射液过敏致喉头水肿20例.云南医药,2004,25(1):59-60
[113]韩朝宏,梁月冬.155例鱼腥草注射液不良反应分析.药物流行病学杂志,2004,13(5):243
[114]张俭.鱼腥草与人体健康.生物学教学,2005,30(8):60
[115]张俊青.鱼腥草的药用和食疗.药膳食疗研究,2000,3:7
[116]邹光友.鱼腥草的食用开发试验.绵阳农专学报,1989,5(3):39-42
[117]杨瑞,张文学,曾里等.鱼腥草饮料的研制开发.四川食品与发酵,2001,(1):42-44
[118]杨小生,康文艺,梁东妮.鱼腥草苦丁茶保健饮料.食品科技,2001,(6):44-45
[119]吴天样,汤庆莉.鱼腥草、南瓜、刺梨醋酸发酵饮料的研制.粮油与食品机械,2001,5:35-36
[120]龚美珍.鱼腥草保健茶饮料的研制.2003,5:74-76
[121]肖玫,刘彪,赵仁铮.鱼腥草天然饮料的工艺研究.西南农业大学学报,2003,25(2):153-156
[122]柯范生等.鱼腥草含片的研制.食品机械,2003,2:25
[123]丁筑红,陈锐,班玉校.鱼腥草袋装方便食品加工.食品机械,1998,5:25
[124]张康健,董娟娥。药用植物次生代谢.西安:西北大学出版社,2001:27-43
[125]Kristina Loziene,Jone Vaiciuniene,Petras R.Venskutonis.Chemical composition of the essential oil of different varieties of thyme(Thymus pulegioides)growing wild in Lithuania[J].Biochemical systematics and ecology,2003,31:249-259
[125]Marc Corticchiato,Felix Tomi,Antoine Franiois Bernardini,et al.Composition and infraspecibc variability of essential oil from Thymus herba barona Lois..Biochemical systematics and ecology,1998,26:915-932
[127]Mauro Marotti,Roberta Piccaglia,Enrico Giovanelli.Differences in Essential Oil Composition of Basil(Ocimum basilicum L.)Italian Cultivars Related to Morphological Characteristics.Journal of Agriculture and Food Chemistry,1996,44:3926-3929
[128]Renee J.Grayer,Geoffrey C.Kite,Fiona J.Goldstone,et al.Infraspecific Taxonomy and Essential Oil Chemotypes in Sweet Basil,Ocimum basilicum.Phytochemistry,1996,43(5):1033-1039
[129]Maria Gorettide Vasconcelos Silva,Francisco Josede Abreu Matos,Maria Iracema Lacerda Machado,et al.Essential oils of Ocimum basilicum L.,O.basilicum,var.minimum L.and O.basilicum,var.purpurascens Benth.grown in north-eastern Brazil.Flavour and Fragrance Journal,2003,18:13-14
[130]Mariateresa Russo,Guido C.Galletti,Paola Bocchini,et al.Essential Oil Chemical Composition of Wild Populations of Italian Oregano Spice(Origanum vulgare ssp.hirtum(Link)Ietswaart):A Preliminary Evaluation of Their Use in Chemotaxonomy by Cluster Analysis.1.Inflorescences.Journal of Agriculture and Food Chemistry,1998,46:3741-3746
[131]M.J.Perez-Alonso,A.Velasco-Negueruela,J.Pala-Paul,et al.Variations in the essential oil composition of Artemisia pedemontana gathered in Spain:chemotype camphor-1,8-cineole and chemotype davanone[J].Biochemical systematics and ecology,2003,31:77-84
[132]Helen D.Skaltsa,Anna Mavrommati,Theophanis Constantinidis.A chemotaxonomic investigation of volatile consitituents in Stachys subsect.Swainsonianeae(Labiatae).Phytochemistry,2001,57:235-244
[133]Marjo Keskitalo,Eija Pehu,James E.Simon.Variation in volatile compounds from tansy (Tanacetum vulgate L.)related to genetic and morphological differences of genotypes.Biochemical systematics and ecology,2001,29:267-285
[134]Yidong Lei,Peng Nan,Tashi Tsering,et al.Interpopulation variability of rhizome essential oils in Rhodiola crenulata from Tibet and Yunnan,China.Biochemical systematics and ecology,2004,32:611-614
[135]Chunjie Tian,Peng Nan,Jiakuan Chen,et al.Volatile composition of Chinese Hippophae rhamnoides and its chemotaxonomic implications.Biochemical systematics and ecology,2004,32:431-441
[136]蔡金娜,张亮,王峥诗,等.蛇床果实中香豆类成分的变异及其规律.药学学报,1999,34:767
[137]周荣汉.国产野生薄荷挥发油化学组分变异及其化学型.植物资源与环境,1998,7(3):13-18
[138]王臣,邢秀芳,马立祥,等.栽培条叶龙胆各形态类型龙胆苦苷含量的测定.中国中药杂志,2004,29(9):841-843
[139]张亮,马国祥,张正行,等.中药石斛质量的化学模式识别.药学学报,1994,29(4):290
[140]秦海林,赵天增.核磁共振氢谱鉴别植物中药的研究.药学学报,1999,34(1):58
[141]秦海林,尚玉俊,赵伟,等.核磁共振氢谱法鉴别黄连研究.中草药,2000,31(1):48
[142]徐朝辉,杨松松,康廷国.同种不同产地牛蒡子DNA指纹图谱特征研究.中草药,2001,32(6):541-542
[143]黄璐琦,王敏,杨滨.栝楼农家品种苗期的分子标记鉴别.中国中药杂志,1999,34(9):642
[144]张荣,张步振,叶浩.用RAPD技术鉴定木蓝属生药.中国中药杂志,1997,22(2):72-73
[145]曹晖,毕培曦,邵鹏柱.中药材苦地胆的DNA指纹鉴定.中药材,1996,19(12):608-611
[146]Shaw P C,But P H.Authentication of Panax species and their adulterants by random-primed polymerase chain reaction.Planta medica,1995,61:466-469
[147]黄璐琦.用随机扩增多态性DNA(RAPD)技术鉴别中药材天花粉及其类似品.药物分析杂志,1999,19(4):233-238
[148]张铭,黄华荣,廖苏梅,等.石斛属RAPD分析及鉴定铁皮石斛的特异性引物设计.中国中药杂志,2001,26(7):442-447
[149]郭兰萍,黄璐琦,王敏,等.南北苍术的RAPD分析机器划分的初步探讨.中国中药杂志,2001, 26(3):156-158
[150]陈毓亨,白守梅,程克棣,等.温郁金和川郁金的RAPD分析.中国中药杂志,1999,24(3):131-133
[151]马小军,汪小全,徐昭玺,等.人参不同栽培群体遗传关系的RAPD分析.植物学报,2000,42(6):587-590
[152]马小军,汪小全,孙三省,等.野生人参RAPD指纹研究.药学学报,1999,34(4):312-316
[153]黄璐琦,王敏,傅桂芳,等.中药白芷种质资源的RAPD分析.中国中药杂志,1999,24(8):457
[154]Yamazaki M,Sato A,Shimomura K,et al.Genetic relationships among Glycyrrhiza plants determined by RAPD and RFLP analysis.Biological and Pharmaceutical Bulletin,1994,17(11):1529-1531
[155]郭宝林,吴孟,斯金平,等.厚朴DNA分子标记的研究-正品的RAPD研究.药学学报,2001,36(5):386-389
[156]陈林姣,屈良鹄,施苏华,等.RAPD技术在溪黄草类原植物鉴别中的应用.中国中药杂志,1998,23:328
[157]陈永久,王文,杨跃雄,等.冬虫夏草(Cordyceps sinensis)的随机扩增多态性DNA及其遗传分化.遗传学报,1997,24:410
[158]Kitanaka S,Kunishi M,Taira T,et al.Ginsenoside production tissue culture and induction of regeneration of interspecific hybrid ginseng(Panax ginseng×P.quinquefolium).Natural Medicines,1997,51(1):1
[159]赵欢,吴卫,郑有良,等.应用RAMP分子标记研究红花资源遗传多样性.植物遗传资源学报,2007,8(1):64-71
[160]Zietkiwicz E,A Rafalski,D Labuda.Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification.Genomics,1994,20:176-183
[161]Gupta M,Chyi Y S,Romero-Severson J,et al.Amplification of DNA markers from evolutionarily diverse genomes using single primers of simple-sequence repeats.Theoretical and Applied Genetics,1994,89:998-1006
[162]Goodwin I D,Aitken E A B,Smith L W.Application of inter-simple sequence repeat(ISSR)markers to plant genetics.Electrophoresis,1997,18:1524-1528
[163]陈祖云,王晓丽,宁聚先.贵州天麻遗传多样性的ISSR初步分析.中华中医药杂志,2007,22(7):436-439
[164]张君毅,郭巧生,杭悦宇.半夏ISSR-PCR反应体系的建立及条件优化研究.江苏农业科学,2007,2:154-157
[165]彭帅,伍贤进,罗碉佶,等.17份鱼腥草种质亲缘关系的ISSR分析.安徽农业科学,2007,35(12):3484-3486
[166]陈志彤,郑伟文,肖华山.泽泻26SrDNA的扩增及酶切反应条件优化.福建农业学报,2004,19(2):117-120
[167]褚必海,丁小余,李雪霞,等.保健食品建泽泻及其混淆品的PCR-RFLP分子鉴别.食品科学, 2007,28(2):177-181
[168]张婷,徐珞珊,王峥涛,等.药用植物束花石斛、流苏石斛及其形态相似种的PCR-RFLP鉴别研究.药学学报,2005,40(8):728-733
[169]康卓.中国生物源农药产业化进展.农药,2001,40(3):4-8
[170]张兴.试论无公害农药.西北农业大学学报,1995,23(6):90-95
[171]何静,沈慧敏,张新虎.七种植物粗提物对常见植物病菌的抑菌作用研究.甘肃农业大学学报,2004.4:447-450
[172]王冬梅,朱玮,张存莉,等.卷叶黄精对植物病原菌的抗菌活性研究.西北植物学报,2006,26(7):1473-1477
[173]黄奔立,朱华,顾艳,等.中草药对5种蔬菜病菌的抑制作用.扬州大学学报(农业与生命科学版),2006,27(3):84-86
[174]熊大胜,席在星,邓应威.鱼腥草提取物抑菌作用研究.常德师范学院学报(自然科学版),2002,14(4):59-60
[175]孟昭礼,吴献忠,高庆霄,等.银杏提取液对4种植物病原菌的抑菌作用.植物病理学报,1995,25(4):357-360
[176]孟昭礼,罗兰,尚坚,等.人工模拟杀菌荆绿帝对8种植物病原菌的室内生测.莱阳农学院学报,1998,15(3):159-162
[177]孟昭礼,罗兰,尚坚,等.人工模拟杀菌剂银泰对10种植物病原菌的室内生测.莱阳农学院学报[J],1999,16(2):124-126
[178]孟昭礼,袁忠林,罗兰,等.人工模拟农用杀菌剂银泰对5种植物害的药效试验.莱阳农学院学报[J],2001,18(4):302-305
[179]孟昭礼,罗兰,袁忠林,等.人工模拟的植物源杀菌剂银泰防治番茄3种病害效果研究.中国农业科学[J],2002,35(7):863-866
[180]Nicolas Baldovini,Dominique Ristorcelli,Felix Tomi,et al.Infraspecific variability of the essential oil of Calamintha nepeta from Corsica(France).Flavour and Fragrance Journal,2000,15,50-54
[181]Giorgio,Pintore;Marianna,Usai;Pascale,Bradesi;et al.Chemical composition and antimicrobial activity of Rosmarinus officinalis L.oils from Sardinia and Corsica.Flavour and Fragrance Journal,2002,17,15-19
[182]Vincenzo,De Feo;Maurizio,Bruno;Bochra,tahiri;et al.Chemical Composition and Antibacterial Activity of EssentialOils from Thymus spinulosus Ten.(Lamiaceae).Journal of Agriculture and Food Chemistry,2003,51,3849-3853
[183]吕世民,粱可钧,葛传吉,等.怀牛膝多倍体育种的研究.中国中药杂志,1988,13(7):11
[184]乔传卓,崔熙.药用植物多倍体的应用.中药材,1981,4:40
[185]乔传卓,吴美枢,戴富宝,等.菘蓝多倍体育种的研究.植物学报,1989,31(9):678
[186]梁汉兴.论三白草科的系统演化和地理分布.云南植物研究,1995,17(3):255-267
[187]黄春燕,吴卫,郑有良,等.两种化学型鱼腥草不同生育期光合速率和挥发油化学成分的比较 研究.中国农业科学,2007,40(6):1150-1158
[188]Sαnchez de la Hoz,M.P.;Davila,J.A.;Loarce,Y.;et al.Simple sequence repeat primers used in polymerase chain reaction amplification to study genetic diversity in barley.Genome,1996,39,112-117
[189]Divaret,I.;Margale,E.;Thomas,G.;et al.RAPD markers on seed bulks efficiently assess the genetic diversity of a Brassica oleracea L.Theoretical and Applied Genetics,1999,98,1029-1035
[190]Nebauer,S.G.;Del CastilloAgudo,L.;Segura,J.;et al.RAPD variation within and among natural populations of outcrossing willow-leaved foxglove(Digitalis obscura L.).Theoretical and Applied Genetics,1999,98,985-994
[191]Parani,M.;Lakshmi,M.;Ziegenhagen,B.,et al.Molecular phylogeny of mangrove.Ⅵ.PCR-RFLP of trnS-psbC and rbcL gene regions in 24 mangrove and mangrove associate species.Theoretical and Applied Genetics,2000,100,456-460.
[192]Xu.D.H.,Abe.A.,Kanazawa.A.,et al.Identification of sequence variations by PCR-RFLP and its application to the evaluation of cpDNA diversity in wild and cultivated soybeans.Theoretical and Appliod Genetics,2001,102,683-688.
[193]Roberto,F.Vieira;Renee,J.Grayer;Alan,Paton;et al.Genetic diversity of Ocimum gratissimum L.based on volatile oil constituents,flavonoids and RAPD markers.Biochemical Systematics and Ecology,2001,29,287-304
[194]S.Echeverrigaray,G.Agostini,L.Atti-Serfini,et al.Correlation between the chemical and genetic relationships among commercial Thyme cultivars.Journal of Agriculture and Food Chemistry,2001,49,4220-4223
[195]Yuan Hu,Qiaoyan Zhang,Hailiang Xin,et al.Association between chemical and genetic variation of Vitex rotundifolia populations from different locations in China:its implication for quality control of medicinal plants.Biomedical Chromatography,2007,21(9):
[196]Madoka Hosoi,Michiho Ito,Toru Yagura,Robert Phillip Adams,et al.cDNA isolation and functional expression of myrcene synthase from Perilla frutescens.Biological and Pharmaceutical Bulletin,2004,27(12):1979-1985
[197]Natalia Dudareva,Diane Martin,Christine M.Kish,et al.(E)-ocimene and myrcene synthase genes of floral scent biosynthesis in Snapdragon:Function and expression of three terpene synthase genes of a new terpene synthase subfamily the plant cell.American Society of Plant Biologists,2003,15,1227-1241
[198]Shan Lu,Ran Xu,Jun-Wei Jia,et al.Cloning and functional characterization of a β-pinene synthase from Artemisia annua that shows a circadian pattern of expressionl plant physiology.American Society of Plant Biologists,2002,130,477-486
[199]Takuro Maruyama,Michiho Ito,Fumiyuki Kiuchi,et al.Molecular cloning,functional expression and characterization of d-Limonene synthase from Schizonepeta tenuifolia.Biological and Pharmaceutical Bulletin,2001,24(4),373-377
[200]王宁生,刘启德,冯美蓉.导数光谱法在益智仁挥发油定性鉴别中的应用.广州中医学院学报, 1990,7(4):225-227
[201]田雅琴,王新霞,高丽红.川芎挥发油稳定性考察.上海中医药杂志,2003,37(7):49-52
[202]黄春燕,吴卫,郑有良.鱼腥草不同部位挥发油化学成分的比较.药物分析杂志,2007,27(1):40-44
[203]朱明华.仪器分析第三版.北京:高等教育出版社,2000:280-282
[204]陈丽艳,崔志恒.植物精油抗菌活性的研究进展.黑龙江医药,2006,19(3):197-198
[205]Cox S D,Mamrn C M,Markham J L.Interactions between components of the essential oil of Melaleuca altermifolia.Journal of Applied Microbial,2001,91(3):492-497
[206]申利群,莫洪波,尹笃林,等.几种单环单萜化合物在农药中的应用.农药,2004,43(4):153-156
[207]秦燕.几种植物精油的抗菌效果研究.食品与机械,1997(2):21-22
[208]钟凤林,陈和荣,陈敏.青蒿最佳采收时期、采收部位和干燥方式的实验研究.中国中药杂志,1997,22(7):405
[209]岳凤先.中药的现代研究.北京:中医古籍出版社,1988:32
[210]杨继祥,李寿乔,齐桂元.药用植物栽培学.北京:农业出版社,1993:344
[211]吴卫,郑有良,马勇,等.鱼腥草不同居群产量和质量分析.中国中药杂志,2003,28(8):718-720
[212]马柏林,梁淑芳,董娟娥,等.杜仲黄酮的微乳薄层色谱鉴定研究.西北林学院学报,2001,16(2):72-74
[213]康纯,闻莉毓,丁仲伯.微乳薄层色谱用于黄酮类成分分离鉴定的研究.药物分析杂志,2000,20(2):121-124
[214]池静端,何秀峰,刘爱茹,等.HPLC法测定银杏叶中6种黄酮成分的含量.药学学报,1997,32(8):625-628
[215]鲁鑫焱,张超,赵怀清,等.不同产地胡芦巴中总黄酮和槲皮素的含量测定.沈阳药科大学学报,2004,21(6):430-433
[216]余红,徐礼.RP-HPLC法测定红花中黄酮醇的含量.药学学报,1997,32(2):120-122
[217]齐迎春,胡诚,等.药食兼用鱼腥草.中国林副特产,1997,12(14):43-45
[218]Owen.R.Fenneman.食品化学.北京:中国轻工业出版社,2003,443
[219]于连贵,李雪花,高山,等.平贝母等3种中药材铅、镉、砷、汞含量的研究.吉林农业大学学报,2001,23(2):53-56
[220]王金平,王晓滨.化妆品、食物等日用品中微、痕量铅、As、Hg的测定.日用化工工业,1997,(6):39-40
[221]聂燕,李缙扬,秦宏伟.应用AFS-2201型双道原子荧光光谱法联合测定饲料中As和Hg.中国饲料,1997(7):32-34
[222]索有瑞.氢化-AFS法测定青藏高原中草药中的微量As、锑、硒和Hg.光谱学与光谱分析,1997,17(5):103-107
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