评估冬训期男子沙滩排球运动员免疫机能的指标研究
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摘要
研究目的:
通过建立一个由T淋巴细胞亚群、自然杀伤细胞(NK)、自然杀伤T细胞(NKT)、外周血皮质醇(C)、睾酮(T)等多个免疫指标构成的检测网络系统,采用流式细胞仪检测等方法,观察男子沙滩排球运动员冬训期训练过程中机体免疫机能的动态变化状况,旨在了解运动员机体免疫机能的变化特点和规律,寻找有意义的免疫指标及有应用价值的检测方法,为更好的掌握运动员对训练方式和运动负荷的适应状况、及时掌握运动员身体机能的变化情况、提高运动训练质量提供依据。
研究方法:
受试者为浙江一线男子沙滩排球运动员10名。冬训期训练周期由二周中等强度大运动量训练、二周大强度中运动量训练及一周中运动强度中运动量恢复训练所构成。以运动训练周期前的调整期作为基础值。于调整期、夏训期训练第2周、第3周、第4周、第5周末运动结束36h后清晨抽取受试者肘静脉血。应用流式细胞仪等检测技术,分别检测外周血TCD3~+、CD4~+、CD8~+、NK细胞、NKT细胞的数目;白细胞及其分类的数量和百分比;外周血C、T的浓度。
研究结果:
1、不同运动训练过程中各免疫指标的变化并不呈现完全相同的趋势。2、运动量与运动强度增加后NKT细胞表达呈现显著下降。3、冬训期运动训练的第4周T淋巴细胞亚群数量明显下降,恢复一周后仍处于较低水平。
结论:
1、NKT细胞在运动训练过程中变化早、变化幅度大,可考虑作为首选的免疫指标运用于运动免疫的检测中。2、外周血C、T的浓度及其比值的变化可有效地反映运动训练量和训练强度的变化。3、运动员进行不同负荷的运动所测得的免疫指标的变化趋势表明:持续时间较长的大负荷运动,有可能引起免疫机能抑制。
Purpose:
The study is intended to establish the immunofunction detecting net which is composed of T cell subsets, natural killer cells (NK), natural killer T(NKT), cortisol and testosterone by advanced measuring techniques——flow cytometry technique. The study aims to investigate the changes of immunofunction in athletes of beach volleyball during winter training session to find out the valuable sensitive immunofunction indexes, and detect the state of athletes during training to make the training better and better.
Methods:
Ten male athletes of beach volleyball participated in the winter training session with two-week training of middle workloads, two-week raining of high workloads, and one-week recovery training of middle workloads. Blood samples were collected in the adjusted period, in the 2nd, 3rd, 4th week during training and after recovery training. Blood samples were assessed by flow cytometry and other ways to determined concentrations of CD3~+, CD4~+, CD8~+, NK, NKT, testosterone and cortisol.
Results:
1. The variatons of immuonfunction indexes did not change simultaneously during training.
2. With the increment of training volume and workloads, the expression of NKT declined.
3. The T cell subsets in the 4th week during winter training session declined significantly, and it was still a low level after recovery.
Conclusion:
1. The NKT may be selected as the best index of evaluating immunofunction of athletes for it was very sensitive in response to training
2. The consistency of testosterone and cortisol can reflect the training value and workloads effectively.
3. It suggested that the immumofunction may be depressed by constant training of high workloads.
通过建立一个由T淋巴细胞亚群、自然杀伤细胞(NK)、自然杀伤T细胞(NKT)、外周血皮质醇(C)、睾酮(T)等多个免疫指标构成的检测网络系统,采用流式细胞仪检测等方法,观察男子沙滩排球运动员冬训期训练过程中机体免疫机能的动态变化状况,旨在了解运动员机体免疫机能的变化特点和规律,寻找有意义的免疫指标及有应用价值的检测方法,为更好的掌握运动员对训练方式和运动负荷的适应状况、及时掌握运动员身体机能的变化情况、提高运动训练质量提供依据。
研究方法:
受试者为浙江一线男子沙滩排球运动员10名。冬训期训练周期由二周中等强度大运动量训练、二周大强度中运动量训练及一周中运动强度中运动量恢复训练所构成。以运动训练周期前的调整期作为基础值。于调整期、夏训期训练第2周、第3周、第4周、第5周末运动结束36h后清晨抽取受试者肘静脉血。应用流式细胞仪等检测技术,分别检测外周血TCD3~+、CD4~+、CD8~+、NK细胞、NKT细胞的数目;白细胞及其分类的数量和百分比;外周血C、T的浓度。
研究结果:
1、不同运动训练过程中各免疫指标的变化并不呈现完全相同的趋势。2、运动量与运动强度增加后NKT细胞表达呈现显著下降。3、冬训期运动训练的第4周T淋巴细胞亚群数量明显下降,恢复一周后仍处于较低水平。
结论:
1、NKT细胞在运动训练过程中变化早、变化幅度大,可考虑作为首选的免疫指标运用于运动免疫的检测中。2、外周血C、T的浓度及其比值的变化可有效地反映运动训练量和训练强度的变化。3、运动员进行不同负荷的运动所测得的免疫指标的变化趋势表明:持续时间较长的大负荷运动,有可能引起免疫机能抑制。
Purpose:
The study is intended to establish the immunofunction detecting net which is composed of T cell subsets, natural killer cells (NK), natural killer T(NKT), cortisol and testosterone by advanced measuring techniques——flow cytometry technique. The study aims to investigate the changes of immunofunction in athletes of beach volleyball during winter training session to find out the valuable sensitive immunofunction indexes, and detect the state of athletes during training to make the training better and better.
Methods:
Ten male athletes of beach volleyball participated in the winter training session with two-week training of middle workloads, two-week raining of high workloads, and one-week recovery training of middle workloads. Blood samples were collected in the adjusted period, in the 2nd, 3rd, 4th week during training and after recovery training. Blood samples were assessed by flow cytometry and other ways to determined concentrations of CD3~+, CD4~+, CD8~+, NK, NKT, testosterone and cortisol.
Results:
1. The variatons of immuonfunction indexes did not change simultaneously during training.
2. With the increment of training volume and workloads, the expression of NKT declined.
3. The T cell subsets in the 4th week during winter training session declined significantly, and it was still a low level after recovery.
Conclusion:
1. The NKT may be selected as the best index of evaluating immunofunction of athletes for it was very sensitive in response to training
2. The consistency of testosterone and cortisol can reflect the training value and workloads effectively.
3. It suggested that the immumofunction may be depressed by constant training of high workloads.
引文
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